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Agaricus bisporus (Mushroom)

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Scientist developed genetically modified mushroom using CRISPR/CAS 9 which helps to reduce browning, which extends the shelf life.

The gene that causes the browning is called polyphenol oxidase (PPO).

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Transmitter: RuvC

PAM: HNH

4) sgRNA was used to guide Cas9 to specific PPO gene sequence in the mushroom.

5) deletion AbPPO4 was knocked out.

Unknown base pair

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Soybean

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2) CRISPR/Cas9

3)The study targeted a transgene (bar) and two endogenous soybean genes (GmFEI2 and GmSHR).

The NGG PAM sequence was used, as required by Cas9.

4)A sgRNA was designed to target specific DNA sequences in soybean hairy roots.

The study designed 1 sgRNA for the transgene (bar) and six sgRNA for endogenous soybean genes

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The study genetically modified Soybean rootsusing CRISPR/Cas9 to enhance disease resistance and increased yield.

The gene modifications targeted specific growth-regulating and stress-responsive genes.

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5) -Target Sequence: (NGG)

-The Cas9 enzyme was directed to a specific target sequence in the soybean genome using a single guide RNA (sgRNA).

-Editing Process: The Cas9-sgRNA complex induced double-strand breaks (DSBs) at the target site.

-Extent of Deletion or Insertion: The mutations introduced varied in size, with most being small indels

6) -Mutation Impact: Gene-edited soybeans showed increased resistance to pathogens by knocking out susceptibility genes and improved tolerance to environmental stress by modifying stress-responsive pathways.

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"Peppa Pig" follows the adventures of the titular, anthropomorphic animal along with her family and friends. Each of her friends is a different kind of animal -- with a last name matching the type of animal each is. Although they are humanlike in many ways, the characters still display characteristics of their species -- such as the Rabbit family being fixated with carrots.

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Peppa is a loveable, cheeky little piggy who lives with her little brother George, Mummy Pig and Daddy Pig. Peppa's favourite things include playing games, dressing up, days out and jumping in muddy puddles. Her adventures always end happily with loud snorts of laughter. Comments are turned off. Learn more Peppa Pig's Secret Box!