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Shmoop Editorial Team. "Biology Animal Respiration - Shmoop Biology." Shmoop. Shmoop University, 11 Nov. 2008. Web. 09 Apr. 2017.

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In this experiment, we places one mouse alone inside an enclosed bio chamber for ten minutes and measured the amount of carbon dioxide inside of the environment using a carbon dioxide probe every minute. We then repeated the experiment using two mice instead of one. As time increased, the amount of carbon dioxide inside of the bio chamber increased. When another mouse was added to the environment, the rate of respiration was higher as compared to the respiration rate of one mouse alone. This indicates that the presence of another mouse increases the respiration rate of each mouse because the oxygen is being shared. Therefore, more breaths are required in order to intake a sufficient amount of oxygen.

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- The purpose of the experiment is to propose the question of whether whether or not animals respire more in groups or alone, an experiment that involutes measuring the rate of cellular respiration.
- The data collected indicates that the amount of carbon dioxide in the air, measured using a carbon dioxide probe, increases every minute when there is one mouse alone as well as when there are two mice together. Though, the overall rate of respiration rate is higher whenever there are two mice together in the same environment. The average respiration rate of mouse 1 alone was  577.1 ppm/minute, whereas the average respiration rate of mouse 1 and 2 together was 821.6 ppm/minute.
- The longer the mouse/mice were inside of the bio chamber, the higher the amount of carbon dioxide inside of the bio chamber (measured in PPM) became. This is an effect of the mouse/mice inhaling the oxygen inside of the enclosed environment, which causes the mouse/mice to exhale carbon dioxide. As time increases, the amount of carbon dioxide inside of the bio chamber rose in that it the environment is enclosed. Therefore, as the mouse continues to respire,  the amount of carbon dioxide present will continue to rise.
- It was hypothesized that if there is another mouse added to the environment of a mouse alone, then the respiration rate of each mouse will decrease slightly. This hypothesis is NOT supported by the data collected in the experiment. The average respiration rate of mouse 1 alone was 577.1 ppm/minute. The average respiration rate of mouse 1 and 2 together was 821.6 ppm/minute. There is a significant increase in the respiration rate when another mouse is added to the environment.
- The data collected indicates that if there are two mice together in an environment as compared to one mouse alone, the respiration rate of each mouse increases. When two mice are sharing oxygen in an enclosed environment, the respiration rate of each moue increases as compared to a single mouse tested in the same environment. This is because in order to intake a sufficient amount of oxygen, the mice must take more breaths because each breath taken is less dense in oxygen than it would be if the mouse were alone.
- In an experiment there are always possible sources of error. In this experiment, one possible source of error is that two different carbon dioxide probes were used to measure the amount of carbon dioxide in the environment every minute. A difference in sensitivity of the probes could have caused an error in accuracy of the data comparisons. Another possible source of error includes the possibility of one of the mice being stressed or agitated, which would increase the mouses respiration rate. If one of the mice was agitated when testing the respiration rate of the two mice together, it could have caused the respiration rate per mouse to increase. The overall amount of carbon dioxide was measured every minute and the results were simply divided by two in order to interpret the collected data and compare respiration rates per mouse. There is also a possibility in the validity of the data in that there could have not been a sufficient amount of trials in order to accurately complete the experiment. The more trials performed in an experiment, the more accurate the data.
- Another experiment or study that would be an extension of this one would be adding plants to the environment of the mice.

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Data Table: Mouse #1 & #2 in Bio Chamber Together

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Data Table: Mouse #1 & #2 in Bio Chamber Together

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Data Table: Mouse #1 Alone in Bio Chamber

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Data Table: Mouse #1 Alone in Bio Chamber

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Bar Graph: The Average Respiration Rate of Mouse #1 & #2 Together

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Bar Graph: The Average Respiration Rate of Mouse #1 & #2 Together

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Line Graph: Average Amount of Carbon Dioxide in Bio Chamber Per Mouse (together)

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Line Graph: Average Amount of Carbon Dioxide in Bio Chamber Per Mouse (together)

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Line Graph: Average Amount of Carbon Dioxide in Bio Chamber Per Minute (alone)

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Line Graph: Average Amount of Carbon Dioxide in Bio Chamber Per Minute (alone)

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1. Put a mouse in the environment alone, making sure it is well cleaned.

2. Wait 2 minutes until beginning the next step.

3. Measure the amount of carbon dioxide in the environment every minute for ten minutes using the carbon dioxide probe then record data.

4. Place the mouse into a beaker.

5. Clean out the environment/container well before placing the mouse back into the environment. Fan probe for thirty seconds.

6. Place the mouse back in the environment.

7. Repeat steps two through five.

8. Place the original mouse as well as the second mouse into the same environment.

9. Repeat steps two through five two times through, making sure to collect your data after each measurement.

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bio chamber container

two mice

carbon dioxide probe

food

water

interface

computer

250 ml beakers

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- In this experiment, the number of mice in a bio chamber is tested with a single mouse alone and two mice together. The expected results are that the respiration rate will slightly decrease with the presence of another mouse.

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- The purpose of cellular respiration, which occurs in the mitochondria, is to harvest energy in glucose (or other organic molecules) and store that energy in ATP. The energy in ATP can then be used to do cellular work as needed. Mice use their lungs to breathe just as humans do, but their lungs are obviously much smaller
- The purpose of this experiment is the test the effect of the presence of a second mouse on carbon dioxide production. The rate of respiration is being tested in order to prove whether or not the respiration rate of mice is affected by the presence of another mouse in the same environment.
- This experiment is important because if there is a large concentration of mice in one ecosystem, then their respiration rates will increase largely. This indicates that there could be potential changes in their internal bodily functions that could cause harm to their health. Furthermore, if population of a species increases as the amount of oxygen producing plants in the environment decreases or stays consistent, there is potential for a possible change in respiration rate.
- The experiment results teach that an increase in the number of mice by one increases the respiration rate per mouse. This indicates that increasing the number of mice in an environment furthermore will cause a more drastic change in respiration rate.