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		About Mr. Mahendra Kumar Trivedi
	

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		About Mr. Mahendra Kumar Trivedi
	

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| Mr. Mahendra Kumar Trivedi has the unique ability to harness the energy from the universe and transmit it to anywhere on earth, infusing it into living and non-living materials to optimize their potential. Read here for the more details.

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	 Mahendra Kumar Trivedi | Biography
	

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	 Mahendra Kumar Trivedi | Biography
	

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| Read here the biography of Mahendra Kumar Trivedi. He is known for his unique biofield energy called "The Trivedi Effect".

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Mahendra Kumar Trivedi | Madhya Pradesh University

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Mahendra Kumar Trivedi,Madhya Pradesh University,Advances in Automobile Engineering .

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		Mahendra Kumar Trivedi's Biofield Energy Treatment
	

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		Mahendra Kumar Trivedi's Biofield Energy Treatment
	

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| Mahendra Kumar Trivedi is well known for his unique approach in the field of a new science called Biofield Energy Treatment. Read here the research papers.

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		 Dr. Rustum Roy Talking About Mahendra Kumar Trivedi
	

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		 Dr. Rustum Roy Talking About Mahendra Kumar Trivedi
	

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| Dr. Rustum Roy talks about the abilities and personality of Mr. Mahendra Kumar Trivedi. Visit the provided link to watch the video.

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Mahendra Trivedi Science Dr. Rustum Roy who is known as the Founder of Material science was a materials scientist who held visiting professorships in materials science at Arizona State University and in medicine at the University of Arizona. He was the Head of the Department of Material Science Department at the Pennsylvania State University, USA.

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		 Hydroxypropyl Cellulose & Hydroxyethyl Cellulose - Biofield Study
	

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		 Hydroxypropyl Cellulose & Hydroxyethyl Cellulose - Biofield Study
	

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| Visit this link to know about how unique human Energy treatment has altered various physicochemical properties of Hydroxypropyl & Hydroxyethyl Cellulose.

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"Influence of Biofield Treatment on Physicochemical Properties of Hydroxyethyl Cellulose and Hydroxypropyl Cellulose" by Mahendra Kumar Trivedi

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Cellulose based polymers have shown tremendous potential as drug delivery carrier for oral drug delivery system (DDS). Hydroxyethyl cellulose (HEC) and hydroxypropyl cellulose (HPC) are widely explored as excipients to improve the solubility of poorly water soluble drugs and to improve self-life of dosage form.

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		 Hydroxypropyl Cellulose XRD Analysis
	

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		 Hydroxypropyl Cellulose XRD Analysis
	

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| The XRD analysis of Hydroxypropyl Cellulose was conducted to observe the changes in its physiochemical properties, induced by Mahendra Trivedi’s unique energy treatment.

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	 Alterations in Hydroxypropyl Cellulose’ Properties
	

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	 Alterations in Hydroxypropyl Cellulose’ Properties
	

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| The present study was conducted to modulate the Hydroxypropyl Cellulose’ properties using the unique human energy treatment. The results showed that the energy treatment has induced positive changes in various properties of HEC and HPC.

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Influence of Biofield Treatment on Physicochemical Properties of Hydroxyethyl Cellulose and Hydroxypropyl Cellulose

# data[].attributes.attachment_link.description
Cellulose based polymers have shown tremendous potential as drug delivery carrier for oral drug delivery system (DDS). Hydroxyethyl cellulose (HEC) and hydroxypropyl cellulose (HPC) are widely explored as excipients to improve the solubility of poorly water soluble drugs and to improve self-life of dosage form.

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		Human Energy Impact on Hydroxyethyl Cellulose’ Properties
	

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		Human Energy Impact on Hydroxyethyl Cellulose’ Properties
	

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| Visit the link to get information about the effects of human energy treatment on Hydroxyethyl Cellulose' properties. Energy treatment had substantially improved the physicochemical properties of HEC and HPC polymers.

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Influence of Biofield Treatment on Physicochemical Properties of Hydroxyethyl Cellulose and Hydroxypropyl Cellulose

# data[].attributes.attachment_link.description
Cellulose based polymers have shown tremendous potential as drug delivery carrier for oral drug delivery system (DDS). Hydroxyethyl cellulose (HEC) and hydroxypropyl cellulose (HPC) are widely explored as excipients to improve the solubility of

# data[].attributes.headline
	
	
	
	
	
		 Investigation of Biofield Effects on Hydroxyethyl Cellulose
	

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		 Investigation of Biofield Effects on Hydroxyethyl Cellulose
	

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| The recent study of Hydroxyethyl Cellulose was carried out to evaluate the impact of biofield treatment on the physicochemical properties of Hydroxyethyl Cellulose.

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		 Hydroxypropyl Cellulose: Study of Biofield Impact
	

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		 Hydroxypropyl Cellulose: Study of Biofield Impact
	

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| The present study was conducted to modulate the physicochemical properties of Hydroxypropyl Cellulose and Hydroxyethyl Cellulose using the biofield treatment.

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		Trivedi Effect | Energy Transformation
	

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		Trivedi Effect | Energy Transformation
	

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| Mahendra Trivedi is a founder of the Trivedi Effect & Trivedi Foundation. He has the unique abilities to harness the energy from the universe and transmit it to anywhere on earth.

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Trivedi Effect - Free downloads and reviews - CNET Download.com

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trivedi effect free download - Advanced Effect Maker Freeware Edition, Photoshop Manga Effect Plugin (32-bit), Paint.NET, and many more programs

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	The Trivedi Effect - A New Scientific Approach
	

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	The Trivedi Effect - A New Scientific Approach
	

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| The Trivedi Effect has proved impact on not only on the human wellness and health disorders but also in scientific researches and experiments, conducted in different fields of science.

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The Trivedi Effect has proved its impact in not only human wellness and health disorders but also in scientific researches and experiments.

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	 The Trivedi Effect | Characterization of Chromium (Vi) Oxide
	

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	 The Trivedi Effect | Characterization of Chromium (Vi) Oxide
	

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| The presentation posted here shows the importance of The Trivedi Effect for altering the characteristics of Chromium (Vi) Oxide.

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Mahendra Kumar Trivedi earned his 5-Year Bachelor's degree in Mechanical Engineering in 1985. Mr. Mahendra Kumar Trivedi worked as an Engineer for 10 Years. In 1995, Mr. Trivedi discovered that he had the unique ability to harness the energy from the universe and transmit it to anywhere on the globe, infusing it into living organisms and nonliving materials, thus optimizing their potential.

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	Research Papers about The Trivedi Effect
	

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	Research Papers about The Trivedi Effect
	

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| Read here all about The Trivedi Effect. Also, find the research papers in which the concept of The Trivedi Effect is used and reported as a new science that could be used to transform the life of the human.

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	 Unique Energy Treatment | The Trivedi Effect
	

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	 Unique Energy Treatment | The Trivedi Effect
	

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| The Trivedi Effect is a natural phenomenon that transforms living organism and non-living materials through the energy transmissions by Mahendra Trivedi.

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The Trivedi Effect®is a unique phenomenon that changes life forms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters™. This helpful...

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		 The Trivedi Effect by Mr. Mahendra Kumar Trivedi
	

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		 The Trivedi Effect by Mr. Mahendra Kumar Trivedi
	

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| Many people have reported that the phenomenon of The Trivedi Effect has transformed their relationships, dramatically increased their happiness and helped them discover their life’s true purpose. This phenomenon is also used positively in hundreds of research papers.

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The Trivedi Effect® is a natural phenomenon that transforms living organisms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters ™. This beneficial transformation is accomplished by strengthening their connection to the source of all creation - Universal Intelligence, enabling them to function at a higher level and serve a greater purpose.

The phenomenon of the 21st Century

The world today is witnessing the emergence of an extraordinary phenomenon that offers a radical way to transform life on this planet for the benefit of all. This phenomenon - brought to the world by Mahendra Trivedi - is known as The Trivedi Effect®.

Mahendra Trivedi - and The Trivedi Masters ™, Dahryn Trivedi, Gopal Nayak and Alice Branton - possess the ability to create The Trivedi Effect® through the Energy Transmissions generated by their thoughts.

Benefits of The Trivedi Effect

The Trivedi Effect ® has the ability to transform all living organisms, including plants, seeds, crops, bacteria, viruses, fungi, animals, cancer cells, and human cells by strengthening their connection to the Universal Energy which is the source of all creation. It is the ONE thing that CAN transform ANY thing.

Tens of thousands of people throughout the world have experienced this phenomenon and are now enjoying the journey of their life.

Astounding the scientific community

The Trivedi Effect® has surprised and astounded the worldwide scientific community. Results from scientific experiments and the vastly growing body of human testimonials have challenged current scientific understanding, pushing scientific thought into new lines of enquiry. Scientific research has shown that The Trivedi Effect® is a natural phenomenon not yet understood by science and is unparalleled in the world as we know it. It is non-invasive in nature and beneficial to all life.

Extensive scientific research

The Trivedi Effect ® has been explored in a number of different subject areas, including agriculture, genetics, cancer, microbiology, material science and animal husbandry through the collaborative efforts of leading scientists from all over the world and the Trivedi Foundation™.

The impact of this phenomenon has been measured, validated and documented in over 4,000 studies at major research institutes throughout the world. Further, these empirical studies have resulted in the publication of numerous substantiating articles in leading international peer-reviewed journals.

Scientific studies performed on non-human and non-living materials subjected to Energy Transmissions have shown dramatic results, demonstrating the fact that the effects of this energy are real and not occurring as a result of a placebo effect. There is no doubt that The Trivedi Effect® has substantial power to change the capabilities of almost everything on this planet.

The Trivedi Effect has:

Increased the yield of crops by up to 500 percent and their immunity by up to 600 percent without the use of any chemicals, fertilizers or pesticides

Identified and killed, in a rigorous, U.S.-based laboratory setting, cancer cells while simultaneously sustaining and promoting greater viability in healthy cells

Reduced the viral load of HIV, Hepatitis B and Hepatitis C, in vitro by up to 99.8 percent

Strengthened the composition of metals and other non-living materials at the atomic level

The Trivedi Effect ® has also brought about health improvements as reported by tens of thousands of individuals - including those with chronic pain, debilitating arthritis, food sensitivities, heart disease, cancer, depression, low self-esteem and more.

Many people have even reported that The Trivedi Effect® has transformed their relationships, dramatically increased their happiness and helped them discover their life's true purpose.

Some benefits are experienced immediately, while others unfold over time. No matter how big or small the benefits seem to be, people everywhere have experienced remarkable and even life-changing results.

If The Trivedi Effect® can do all this, imagine what it could do for you!

Brain MRI

Thermal Imaging

Spine MRI

" The Augmentation and Aortic reflection show the amount of stiffness seen in a person 20-25 years younger"
-Dr. John Levin, Anti-Aging Specialist, Australia

" The pituitary fossa is the largest ever found in a healthy subject with fully functioning body having excellent health"
-Dr Shailendra Patil, India

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The Trivedi Effect® | Mahendra Trivedi | Human Anatomy and Physiology

# data[].attributes.attachment_link.description
The Trivedi Effect ® is a natural phenomenon that transforms living organisms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters ™. This beneficial transformation is accomplished by strengthening their connection to the source of all creation - Universal Intelligence, enabling them to function at a higher level and serve a greater purpose.

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		Impact of Unique Energy Treatment on Viral Load of Hepatitis B and C Viruses
	

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		Impact of Unique Energy Treatment on Viral Load of Hepatitis B and C Viruses
	

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| Visit the link to read the impact of unique human energy treatment on Viral Load of Hepatitis B and C Viruses. The results suggest that his energy medicine could be a suitable alternative treatment strategy for hepatitis patients in near future.

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		 Alternative Treatment to Alter HCV RNA Viral Load Count
	

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		 Alternative Treatment to Alter HCV RNA Viral Load Count
	

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| The findings of the present study suggest that the unique energy treatment has significantly reduced the HCV RNA viral load count. Read here for more details.

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Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses

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Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of hepatitis.

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		Alternative Treatment to Alter HCV RNA Viral Load Count
	

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		Alternative Treatment to Alter HCV RNA Viral Load Count
	

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	Biofield Treatment Study using COBAS Amplicor HCV Monitor Test
	

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	Biofield Treatment Study using COBAS Amplicor HCV Monitor Test
	

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| COBAS Amplicor HCV Monitor Test was conducted to understand the influence of unique energy treatment on Hepatitis B and C Viruses. Visit to know about the positive results.

# data[].attributes.attachment_link.title
Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses

# data[].attributes.attachment_link.description
Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular

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		Hepatitis B Viral Load Test of Human Energy Treated HBV
	

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		Hepatitis B Viral Load Test of Human Energy Treated HBV
	

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| In this study, Hepatitis B viral load test was performed on the samples before and after the application of unique healing energy and evaluated the results. The same tests were performed on the Hepatitis C viruses as well.

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	 Energy Medicine to Reduce the HCV RNA Viral Load Count
	

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	 Energy Medicine to Reduce the HCV RNA Viral Load Count
	

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| Read here the impact of unique energy medicine on HCV RNA Viral Load Count. The human energy treatment has significantly reduced the viral load count of HCV.

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		 HEP C Viral Load – An Impact of Human Energy Treatment
	

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		 HEP C Viral Load – An Impact of Human Energy Treatment
	

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| The aim of this study was to evaluate the impact of biofield modality on Hepatitis B virus (HBV) and Hepatitis C virus (HCV) in terms of viral load as a surrogate marker. Follow the links for full information.

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	 Nocardia Otitidis Antimicrobial Susceptibility Analysis
	

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	 Nocardia Otitidis Antimicrobial Susceptibility Analysis
	

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| Antimicrobial susceptibility analysis of Nocardia otitidis was conducted to evaluate the impact of spiritual healing energy treatment on it. Visit the link to know more about the study and positive results.

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phylogenetic analysis of nocardia otitidis

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"Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis" by Mahendra Kumar Trivedi

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Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

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	 Human Energy Treatment & Phylogenetic Analysis of Nocardia Otitidis
	

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	 Human Energy Treatment & Phylogenetic Analysis of Nocardia Otitidis
	

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| The present study shows the evaluation of antibiogram, genotype and phylogenetic analysis of Nocardia otitidis in the presence of unique human energy treatment. Various methods were used to analyze the impact of human energy treatment on Nocardia otitidis samples.

# data[].attributes.attachment_link.title
Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate

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		 Nocardia Otitidis DNA Polymorphism Analysis after Biofield Treatment
	

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		 Nocardia Otitidis DNA Polymorphism Analysis after Biofield Treatment
	

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| There is an impact of biofield treatment on MIC, 16S rDNA analysis, and DNA polymorphism of Nocardia otitidis, suggested the results of this study which was conducted to evaluate the impact of biofield energy on Nocardia otitidis.

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		 Antimicrobial Susceptibility of Nocardia Otitidis
	

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		 Antimicrobial Susceptibility of Nocardia Otitidis
	

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| This study was conducted to investigate the effect of unique human energy on N. otitidis samples and analyzed for antimicrobial susceptibility pattern, MIC values, DNA polymorphism by RAPD analysis and 16S rDNA sequencing. Visit to know more.

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	Biofield Impact on Klebsiella Pneumoniae Characteristics
	

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	Biofield Impact on Klebsiella Pneumoniae Characteristics
	

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| This study evaluates the impact of biofield treatment on biochemical characteristics of Klebsiella Pneumoniae characteristics such as biochemical reactions, biotype numbers and etc.

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Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

Research ArticleOpen Access

Trivedi et al.,J Trop Dis 2015, 3:4

http://dx.doi.org/10.4173/2329-891X.1000173

Research Article Open Access

Journal of Tropical Diseases

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Harish Shettigar1, Mayank Gangwar2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Citation: Trivedi MK, Branton A, Trivedi D, Shettigar H, Gangwar M, et al. (2015)

Antibiogram Typing and Biochemical Characterization of Klebsiella Pneumonia

after Bioeld Treatment. J Trop Dis 3: 173. doi:10.4173/2329891X.1000173

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Keywords: Klebsiella pneumoniae; Bioeld Treatment; Multidrug-

Resistant; Antimicrobial susceptibility; Biochemical Reaction;

shaped, facultative anaerobic, and nonmotile bacterium, belongs to

family Enterobacteriaceae . It is a common human pathogen associated

with nosocomial and community infections [1]. K. pneumoniae isolates

causes several infections such as pneumonia, septicemia, wound

infections, and urinary tract infections, which ultimately lead to

morbidity and mortality especially in immunocompromised patients,

and patients of intensive care units, pediatrics and surgical wards [2].

K. pneumoniae acquire resistance against existing antimicrobials by

multiple mechanism results in increased multidrug-resistant (MDR)

of K. pneumoniae thatleads to serious problem in hospital settings

and health concern. Emergence of resistance occurs not only in

MDR isolates but also exist in pan-drug resistant (PDR) isolates of K.

pneumoniae. PDR refers to the resistant strains those are specically

resistant to 7 antimicrobial agents such as cefepime, imipenem,

meropenem, ceazidime, ciprooxacin, piperacillin-tazobactam,

and levooxacin [3]. Apart from this, the extended-spectrum

β-lactamase (ESBL) producing Klebsiella from a patient has been

identied which causes serious threat worldwide [4,5]. Continuous

use of antibiotics leads to resistance in microorganisms via. dierent

pathways mediated by plasmids, transposons, and gene cassettes in

integrons [6,7]. Carbapenem is usually preferred for the infection

caused by MDRisolates of K. pneumoniae but recently carbapenem-

resistant K. pneumoniae was also reported [8]. Due to dramatically

increase in drug resistant in K. pneumoniae, very few treatment

options are available. Alternative approaches are available but altering

the sensitivity pattern of antimicrobials using bioeld is not available

against MDR microorganism, apart from existing allopathic system of

medicine. Bioeld treatment may be an alternative approach to alter

the susceptibility pattern of K. pneumoniae. Complementary and

alternative medicine (CAM) therapies are commonly practiced in

healthcare sector and about 36% of Americans regularly uses some form

of CAM [9]. CAM include numerous energy therapies, bioeld therapy,

is one of the energy medicine widely used worldwide to improve the

human health. e energy exists in various forms that can be produced

from dierent sources such as potential, electrical, kinetic, magnetic,

and nuclear energy. However, electromagnetic eld denes as when

electrical signals uctuate will generate magnetic eld with respect

to time. e cumulative eect of bio-magnetic and electric eld that

surrounds the human body is dened as bioeld. e bioeld energy can

be monitored by using electromyography (EMG), electrocardiography

(ECG) and electroencephalogram (EEG) [10]. According to Lucchetti

et al. bioeld energy has shown signicant eect on growth of bacterial

cultures [11]. Mr. Trivedi has the ability to harness the energy from

environment or universe and can transmit into any living or nonliving

object(s) around the Universe. e objects always receive the energy

and responding into useful way via bioeld energy and the process is

known as bioeld treatment. Mr. Trivedi's unique bioeld treatment is

extensively studied in dierent elds such as in material science [12,13],

agricultural science [14-16], and in biotechnology [17]. Further,

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae)

patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's bioeld treatment

on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into

two groups i.e. control and bioeld treated. Control and treated groups were analyzed for antimicrobial susceptibility

pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away®

system. The analysis was done on day 10 after bioeld treatment as compared with control group. Antimicrobial

sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of

treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was

reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in

biochemical reactions as compared to control. A signicant change in biotype numbers were reported in all four clinical

number after bioeld treatment, new organism was identied as Enterobacter aerogenes in LS 2 and LS 14. These

results suggest that bioeld treatment has a signicant effect on altering the antimicrobial sensitivity, MIC values,

biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

the bioeld treatment has considerably altered the susceptibility of

antimicrobials and biotype of microbes [18-20]. By considering the

above mentioned facts and literature reports on bioeld treatment,

the present work was undertaken to evaluate the impact of bioeld

treatment on antimicrobials susceptibility, biochemical reactions

pattern, and biotype of MDR isolates of K. pneumoniae.

Experimental design and bacterial isolates

MDR clinical lab isolates (i.e. LS 2, LS 6, LS 7 and LS 14) of K.

pneumoniae were obtained from stored stock cultures in Microbiology

Lab, Hinduja Hospital, Mumbai. Each MDR strains was divided

into two groups i.e. control and treatment. e acceptability of the

identication media and antimicrobial agents were checked prior to the

study on microorganisms. e antimicrobial susceptibility, biochemical

reactions, and biotype number were evaluated on MicroScan Walk-

Away® (Dade Behring Inc., West Sacramento, CA) using Negative

Breakpoint Combo 30 (NBPC 30) panel. e NBPC 30 panel was stored

at 2 to -25ºC. All antimicrobials and biochemicals were procured from

Bioeld treatment strategy

Treatment groups of each strain, in sealed pack were handed over

to Mr. Trivedi for bioeld treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated groups without touching the samples. e bioeld treated

samples were returned in the similar sealed condition and analyzed

on day 10 using the standard protocols. e study was conducted on

automated MicroScan Walk-Away® system (Dade Behring Inc., USA).

Evaluation of antimicrobial susceptibility assay

Antimicrobial susceptibility patterns of MDR lab isolates of K.

pneumoniae were studied using MicroScan Walk-Away® using Negative

Break Point Combo (NBPC 30) panel as per manufacturer's instructions.

e antimicrobial susceptibility pattern (S: Susceptible, I: Intermediate,

IB: Inducible β-lactamase; EBL: Suspected extended-spectrum β

-lactamases, and R: Resistant) and MIC values were determined by

observing the lowest antimicrobial concentration showing growth

inhibition [21]. e antimicrobials used in the susceptibility assay viz.

amikacin, amoxicillin/k-clavulanate, ampicillin/sulbactam, ampicillin,

aztreonam, cefazolin, cefepime, cefotaxime, cefotetan, cefoxitin,

ceazidime, ceriaxone, cefuroxime, cephalothin, chloramphenicol,

ciprooxacin, gatioxacin, gentamicin, imipenem, levooxacin,

meropenem, moxioxacin, noroxacin, nitrofurantoin piperacillin,

piperacillin/tazobactam, tetracycline, ticarcillin/K-clavulanate,

tobramycin, and trimethoprim/sulfamethoxazole

Biochemical studies of each MDR isolates of K. pneumoniae were

determined by MicroScan Walk-Away® using NBPC 30 panel system

in both control and treated groups. Biochemicals used in the study

are acetamide, adonitol, arabinose, arginine, cetrimide, cephalothin,

citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose, hydrogen

sulde, indole, inositol, kanamycin, lysine, malonate, melibiose, nitrate,

oxidation-fermentation, galactosidase, ornithine, oxidase, penicillin,

ranose, rhamnose, sorbitol, sucrose, tartarate, tryptophan deaminase,

tobramycin, urea, and Voges-Proskauer [21].

Identication by biotype number

e biotype number of each MDR isolates of K. pneumoniae in

control and treated sample were determined followed by identication

of microorganism by MicroScan Walk-Away® processed panel data

report with the help of biochemical reaction data [21].

Antimicrobial susceptibility study

Results of antimicrobial sensitivity pattern and MIC values of

control and treated MDR isolates of K. pneumoniae are summarized

in Tables 1 and 2, respectively. All these changes were observed on 10

days aer bioeld treatment as compared to control group. Overall,

46.42% of tested antimicrobials out of twenty eight, showed alteration

in antimicrobial sensitivity pattern against bioeld treated MDR

isolates of K. pneumoniae. All four MDR isolates, showed variations

in antimicrobial sensitivity assay viz. 32.14% in LS 2, 25% in LS 6,

17.85% in LS 7, and 28.57% in LS 14 against the tested antimicrobials

(Figure 1). Extended spectrum beta-lactamases (ESBLs) are rapidly

evolved group of beta-lactamases enzyme, which confer resistance to

most beta-lactam antibiotics, including penicillins, cephalosporins,

monobactam and aztreonam. Apart from beta-lactam antibiotics,

ESBLs are also resistant to other classes of non-penicillin antibiotics

[22]. Beta-lactamases are enzymes that inactivates the antibiotic and are

present in almost all Gram-negative bacilli. However, some pathogenic

production of β-lactamase which varies from low to high level. In

some species, exposure to β-lactams will induced the production level

of β-lactamase, commonly results in resistance to these agents. ese

inducible β-lactamases are frequently found in Enterobacter spp. [23].

Experimental results of antimicrobial sensitivity assay showed altered

sensitivity pattern in bioeld treated clinical isolates of K. pneumonia .

Aztreonam, cefotaxime, ceazidime, and ceriaxone sensitivity

changed from EBL → R in LS 2 and LS 14. Sensitivity of amoxicillin/k-

clavulanate changed from S → R in LS 7 and LS 14, while S → IB and

I → R in LS 2 and LS 6 respectively. Cefotetan and cefoxitin found

changed sensitivity pattern from S → R in LS 7, and while S → IB in

LS 2. Sensitivity of cefotetan changed from I → R in LS 6 while S →

IB in LS 14. Chloramphenicol and imipenem sensitivity changed from

S → R in LS 6. Although, in imipenem sensitivity changed from S →

I in LS 6. Meropenem sensitivity changed from I → R and S → R in

LS 6 and LS 7 respectively. An increase in sensitivity was reported in

piperacillin/tazobactam and piperacillin i.e. from R → I in bioeld

treated LS 6 as compared to control. Although, piperacillin/tazobactam

sensitivity changed from S → IB and S → I in LS 2 and LS 14 respectively.

Ticarcillin/K-clavulanate sensitivity altered from S → I and S → R in LS

2 and LS 14 respectively (Table 1). Rest of antimicrobials did not show

any change in sensitivity pattern aer bioeld treatment.

Determination of Minimum Inhibitory Concentration (MIC)

MIC values of all the clinical MDR isolates of control and bioeld

treated K. pneumoniae are summarized in Table 2. MIC values were

decreased in case of piperacillin/tazobactam and piperacillin in LS

6 isolate only, while in rest of the antimicrobials, MIC values were

increased as compared to control. Overall, MIC results showed an

alteration in 30% tested antimicrobials (i.e. nine out of thirty) aer

bioeld treatment in clinical isolates of K. pneumoniae (Table 2 and

Figure 1). A decreased in MIC values in piperacillin/tazobactam and

piperacillin were reported along with increases antimicrobial sensitivity

aer bioeld treatment (64 µg/ml) in LS 6. Current treatment strategy

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

S. No.AntimicrobialLS 2LS 6LS 7LS 14

8 CefotaximeEBLREBLEBL EBLEBLEBLR

C: Control; T: Treatment; R: Resistant; I: Intermediate; S: Susceptible; LS: Lab Isolate; EBL: Suspected extended-spectrum beta-lactamases

against K. pneumoniae infections preferably uses cefoperazone/

sulbactam, piperacillin/tazobactam, and imipenem antimicrobials

[24]. Although, piperacillin/tazobactam antimicrobial agent is useful

and preferred in neonatal infections caused due to K. pneumoniae [25].

Bioeld treatment in clinical isolate (LS 6) signicantly increased the

sensitivity and decreased the MIC values of piperacillin/tazobactam

and piperacillin. In Enterobacteriaceae family, the most prevalent

mechanism of acquired resistance in β-lactam antibiotics (piperacillin/

tazobactam and piperacillin) are the production of β-lactamases [26].

Bioeld treatment might act on enzymatic or genetic level which might

aects the β-lactamases production that may lead to alter the sensitivity

pattern of tested antimicrobials.

Biochemical and biotype number study

Biochemical study results of control and bioeld treated groups

are summarized in Table 3 and Figure 1. Results showed that overall

15.15% change in tested biochemical reactions among 4 treated MDR

clinical isolates of K. pneumoniae as compared to control. Cetrimide

changed from (-) negative to (+) positive reaction in LS 6, LS 7, and LS

14 as compared to control. Voges-Proskauer changed from (+) positive

to (-) negative reaction in LS 2, LS 6, and LS 7. Indole changed from

(+) positive to (-) negative reaction in LS 7. Ornithine changed from

negative (-) to positive (+) reaction in LS 14. Rest of biochemicals did

not show any alteration in their reaction aer bioeld treatment. Voges-

Proskauer, citrate, arabinose, lysine, glucose, sucrose, malonate are the

standard positive reaction biochemical tests of K. pneumoniae while

hydrogen sulde, indole, ornithine and cetrimide are the standard

negative reaction test. Biochemical reactions of control MDR isolates

of K. pneumoniae were well supported with literature data [27]. Based

on the biochemical results, signicant alteration in biotype numbers

were observed in all the four bioeld treated lab isolates i.e. LS 2, LS 6,

LS 7, and LS 14 as compared to control. New organism was identied

as Enterobacter aerogenes in LS 2 and LS 14 aer bioeld treatment

on day 10 with respect to control (Table 4). Bioeld treatment as

an alternate and complementary medicine, increasingly used in

biomedical health care system such as reduction in pain and anxiety

[28]. However, National Center for Complementary and Alternative

Medicine/National Institute of Health (NCCAM/NIH), now dened

bioeld therapies in subcategory of energy therapies as one of the

ve complementary medicine domain [29]. Mr. Trivedi's bioeld

treatment in pathogenic microbes were extensively studied and had

shown signicant alteration in the antimicrobial sensitivity pattern,

biochemical reactions, and biotype number [18,19]. Bioeld treatment

might be responsible to do alteration in microorganism at genetic level

and/or enzymatic level, which may act on receptor protein. While

altering receptor protein, ligand-receptor/protein interactions may

also alter that could lead to show dierent phenotypic characteristics.

Hence a cascade of intra-cellular signals may be initiated, accelerated or

inhibited [30]. e overall observations showed that, bioeld treatment

on MDR isolates of K. pneumoniae induced signicant alteration in

antimicrobial susceptibility pattern, MIC values, biochemical reactions,

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

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		 Healing Energy and Changes in Thermal Properties of Cadmium
	

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		 Healing Energy and Changes in Thermal Properties of Cadmium
	

# data[].attributes.body
| The DSC data suggest that the healing energy treatment has significantly altered the thermal properties of Cadmium powder. The results show that the latent heat of fusion was reduced significantly as compared to the untreated samples.

# data[].attributes.attachment_link.title
"An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

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		 Biofield Impact on the Physical Properties of Cadmium
	

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		 Biofield Impact on the Physical Properties of Cadmium
	

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| Follow the link to know about the impact of biofield treatment on the structural, thermal and physical properties of Cadmium. This study has outlined some excellent results.

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		Cadmium Powder Particle Size Analysis
	

# data[].attributes.subject
	
	
	
	
	
		Cadmium Powder Particle Size Analysis
	

# data[].attributes.body
| It has been observed that the unique energy healing treatment of Cadmium powder has resulted in substantial reduction in average particle size and increased surface area. Thus, the treated Cadmium could show an improved kinetics of electrochemical reactions.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

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		 Alterations in Structural Properties of Cadmium Powder
	

# data[].attributes.subject
	
	
	
	
	
		 Alterations in Structural Properties of Cadmium Powder
	

# data[].attributes.body
| The results of this study revealed that the biofield treatment has significantly altered the atomic and structural properties of cadmium powder. The biofield treatment has also resulted in alterations in the physical & thermal properties of Cadmium.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical

# data[].attributes.headline
	
	
	
	
	 Healing Energy Impact of on the Thermal Properties of Cadmium
	

# data[].attributes.subject
	
	
	
	
	 Healing Energy Impact of on the Thermal Properties of Cadmium
	

# data[].attributes.body
| The differential scanning calorimetry (DSC) analysis method was used to analyze the impact of unique energy healing treatment on the various thermal properties of Cadmium.

# data[].attributes.headline
	
	
	
	
	 Structural, Thermal and Physical Properties of Cadmium
	

# data[].attributes.subject
	
	
	
	
	 Structural, Thermal and Physical Properties of Cadmium
	

# data[].attributes.body
| The study was conducted to evaluate the impact of human energy treatment on atomic, thermal, and physical properties of Cadmium Powder. Read here for more details and to know about the results of various activities.

# data[].attributes.headline
	
	
	
	
	
		 Energy Healing Therapy & Physical Properties of Cadmium Powder
	

# data[].attributes.subject
	
	
	
	
	
		 Energy Healing Therapy & Physical Properties of Cadmium Powder
	

# data[].attributes.body
| Read here the impact of unique human energy treatment on the thermal, physical and structural properties of Cadmium. The data shows significant alterations in various characteristics of Cadmium Powder.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder - PdfSR.com

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

# data[].attributes.headline
	
	
	
	
	 Serratia Marcescens Biochemical Tests
	

# data[].attributes.subject
	
	
	
	
	 Serratia Marcescens Biochemical Tests
	

# data[].attributes.body
| The study and different biochemical tests of Serratia marcescens resulted that the biofield treatment can prevent the emergence of absolute resistance to the useful antimicrobials against S. marcescens.

# data[].attributes.headline
	
	
	
	
	 Study of Stenotrophomonas Maltophilia Antibiotic Susceptibility
	

# data[].attributes.subject
	
	
	
	
	 Study of Stenotrophomonas Maltophilia Antibiotic Susceptibility
	

# data[].attributes.body
| Stenotrophomonas maltophilia was analyzed for changes in Antibiotic Susceptibility and other biochemical characteristics. Visit to get more information.

# data[].attributes.attachment_link.title
Peer Evaluation : An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen

# data[].attributes.attachment_link.description
Abstract : Stenotrophomonas maltophilia (S. maltophilia) is a Gram-negative bacillus, an opportunistic pathogen, particularly among nosocomial infections. Multi-drug resistant strains are associated with very high rate of morbidity and mortality in severely immunocompromised patients. Present study was designed to evaluate the effect of biofield treatment against multidrug resistant S. maltophilia.

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	Sensitivity Pattern of Stenotrophomonas Maltophilia Antimicrobials
	





	

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	Sensitivity Pattern of Stenotrophomonas Maltophilia Antimicrobials
	





	

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| Unique healing therapy has significantly altered the sensitivity pattern of Stenotrophomonas Maltophilia Antimicrobials and other characteristics. Read more here.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen | Mahendra Trivedi | Academic Room

# data[].attributes.headline
	
	
	
	
	 Effects of Unique Energy Treatment on S. Maltophilia Genotype
	

# data[].attributes.subject
	
	
	
	
	 Effects of Unique Energy Treatment on S. Maltophilia Genotype
	

# data[].attributes.body
| The study present here shows the significant impact of unique energy healing treatment on the Genotypes of S. Maltophilia. Visit to get more information.

# data[].attributes.attachment_link.title
"An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Stenotrophomonas maltophilia (S. maltophilia) is a Gram-negative bacillus, an opportunistic pathogen, particularly among nosocomial infections. Multi-drug resistant strains are associated with very high rate of morbidity and mortality in severely immunocompromised patients. Present study was designed to evaluate the effect of biofield treatment against multidrug resistant S. maltophilia. Clinical sample of S.

# data[].attributes.headline
	
	
	
	
	
		Multidrug Resistant of S. Maltophilia & Unique Healing Therapy
	

# data[].attributes.subject
	
	
	
	
	
		Multidrug Resistant of S. Maltophilia & Unique Healing Therapy
	

# data[].attributes.body
| The study was conducted to evaluate the impact of unique energy treatment on multidrug resistant of S. Maltophilia and significant changes were noticed in various characteristics of S. Maltophilia.

# data[].attributes.headline
	
	
	
	
	
		 S. Maltophilia Susceptibility Analysis with Unique Energy Treatment
	

# data[].attributes.subject
	
	
	
	
	
		 S. Maltophilia Susceptibility Analysis with Unique Energy Treatment
	

# data[].attributes.body
| S. Maltophilia susceptibility analysis using unique energy treatment has induced significant changes in antimicrobial susceptibility of MDR of S. Maltophilia. Read complete research paper for more details.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Susceptibility Pattern of Multidrug Resistant Stenotrophomonas maltophilia: An Emerging Global Opportunistic Pathogen

# data[].attributes.attachment_link.description
Stenotrophomonas maltophilia (S. maltophilia) is a Gram-negative bacillus, an opportunistic pathogen, particularly among nosocomial infections. Multi-drug resistant strains are associated with very high rate of morbidity and mortality in severely

# data[].attributes.headline
	
	
	
	
	
		Effects of Biofield Energy on Stenotrophomonas Maltophilia Antibiotic Susceptibility
	

# data[].attributes.subject
	
	
	
	
	
		Effects of Biofield Energy on Stenotrophomonas Maltophilia Antibiotic Susceptibility
	

# data[].attributes.body
| The present study was undertaken to evaluate the impact of biofield treatment on MDR strain of S. maltophilia. The change in antimicrobial susceptibility pattern, biochemical reactions, and others were studied.

# data[].attributes.headline
	
	
	
	
	
		 Study on Stenotrophomonas Maltophilia Susceptibility Pattern Changes   
	

# data[].attributes.subject
	
	
	
	
	
		 Study on Stenotrophomonas Maltophilia Susceptibility Pattern Changes   
	

# data[].attributes.body
| Unique human energy treatment has the ability to alter the susceptibility pattern of S. Maltophilia. In a recent study, a change of 37.5% in susceptibility pattern and 33.3% in MIC values of S. Maltophilia was measured.

# data[].attributes.headline
	
	
	
	
	 Biofield Treated Bile Salt and Proteose Peptone
	

# data[].attributes.subject
	
	
	
	
	 Biofield Treated Bile Salt and Proteose Peptone
	

# data[].attributes.body
| Take a look on the research about Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone. For more publication about Biofield Energy visit on www.trivediscience.com.

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¹ Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

² Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

*Corresponding Author:

Snehasis Jana
Trivedi Science Research Laboratory Pvt. Ltd
Hall-A, Chinar Mega Mall, Chinar Fortune City
Hoshangabad Rd., Bhopal-462026
Madhya Pradesh, India
Tel: +91-755-6660006
E-mail: [email protected]rl.com

Received date: July 06, 2015; Accepted date: July 16, 2015; Published date: July 23, 2015

Citation: Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone. J Anal Bioanal Tech 6:256. doi: 10.4172/2155-9872.1000256

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Analytical & Bioanalytical Techniques

Abstract

Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated BS and PP samples were characterized by particle size analyzer (PSA), Brunauer-Emmett-Teller (BET) analysis, differential scanning calorimetry (DSC), x-ray diffraction (XRD), and thermogravimetric analysis (TGA). PSA results showed increase in particle size (d50 and d99) of both treated BS and PP as compared to control. Surface area analysis showed minimal decrease by 1.59%, in surface area of treated BS as compared to control. However, the treated PP showed increase (8%) in surface area as compared to control. DSC characterization showed increase in melting temperature of treated BS as compared to control. Whereas, DSC thermogram of treated PP showed decrease in melting temperature with respect to control. Moreover, the DSC of control and treated PP showed presence of exothermic peaks which were possibly due to protein aggregation. The treated PP showed higher exothermic transition temperature as compared to control. XRD analysis revealed slight reduction in crystalline nature of BS as compared to control. On the other hand, XRD data of control and treated PP showed an amorphous nature. TGA analysis of treated BS showed maximum thermal decomposition temperature at 22°C which was higher as compared to control sample (106°C). This could be due to biofield treatment which may enhance the thermal stability of treated BS with respect to control. However, the TGA thermogram of treated PP showed decrease in maximum thermal stability as compared to control. The overall results showed that biofield treatment has significantly altered the physical and thermal properties of BS and PP.

Keywords

Bile salt; Proteose peptone; Particle size; Brunauer- Emmett-Teller analysis; X-ray diffraction; Differential scanning calorimetry; Thermogravimetric analysis

Abbreviations

PSA: Particle Size Analyzer; BET: Brunauer- Emmett-Teller analysis; DSC: Differential Scanning Calorimetry; XRD: X-ray Diffraction; TGA: Thermogravimetric Analysis; DTA: Differential Thermal Analyzer; DTG: Derivative Thermogravimetry; BS: Bile Salt; PP: Proteose Peptone

Introduction

Bile salts (BS) are commonly known as bio-surfactants that plays crucial physiological role in human gastro intestinal tract such as fat digestion and absorption of nutrients and also serve as a mean for removal of waste products from blood [ 1, 2]. Briefly, BS acts as a carrier for fat soluble products due to its ability of forming micelles with phospholipids. Moreover, the BS plays an important role in nutrition by improving solubility and transport of fat soluble nutrients to the mucosa of small intestine. Based on chemical nature of BS are flat molecules with both hydrophilic and hydrophobic faces [ 3]. Many literature reports provided interesting information about the selfassembly nature of BS in solution suggesting the fascinating properties of BS aggregates as compared to conventional surfactants [ 4- 6]. Due to this micellar nature of BS, which enables solubilization and transport of lipid soluble compounds thus it helps in fat digestion. Therefore, this same biological function can be exploited for pharmaceutical application since most drugs currently in development have low water solubility [ 1]. Thus BS based carrier systems are promising for specific targeting and absorption of non-soluble compounds. However, it was shown that BS is a poor surface active-agent compared to other commonly used surfactants such as dodecyl sulfate and sodium dodecanoate. Hence, in order to improve these properties BS should be modified in order to confer better physicochemical properties.

On the other hand proteose peptone (PP) is obtained from bovine milk which is partially consist of a number of heat stable minor proteins, glycoproteins, and largely of casein derived peptides [ 7, 8]. These are generated in human body by the action of proteinases (mainly plasmin) of all the four main casein proteins [ 9- 11]. These protein compounds require proper modification in order to alleviate its properties which can be utilized for further applications.

Scientists have demonstrated that short lived electrical events or action potential occurs in several types of mammalian cells such as neurons, muscle cells, and endocrine cells [ 12]. For example, the cells in the nervous system communicate with each another by means of electrical signals that travel along the nerve processes. Therefore, it is hypothesized that biofield exists around the human body and the evidence can be found using medical technologies such as Electromyography, Electrocardiography and electroencephalogram [ 13].

Thus, human has the ability to harness the energy from environment or universe and can transmit into any living or nonliving objects around the Globe. The objects always receive the energy and responding into useful way that is called biofield energy and the process is known as biofield treatment. Recently, biofield energy has shown significant effect on structural, crystalline and thermal properties of various metals, ceramics and carbon allotropes [ 14- 17].

Mr. Mahendra K. Trivedi is known to transform the properties of various living and non-living things under controlled experiments using his unique biofield energy. Biofield treatment had substantially changed the atomic, crystalline, surface properties of various materials. The biofield had significantly changed the overall productivity and quality in the field of agriculture and biotechnology [ 18- 21]. Additionally, biofield has shown excellent results in improving antimicrobial susceptibility, and alteration of biochemical reactions, as well as induced alterations in characteristics of pathogenic microbes [22- 24]. The biofield had also caused an increase in growth and anatomical characteristics of an herb Pogostemon cablin that is commonly used in perfumes, in incense/insect repellents, and alternative medicine [ 25].

In the present study, the influence of biofield treatment on physicochemical properties of BS and PP were studied with the aid of different methods like particle size analyzer (PSA), Brunauer-Emmett- Teller (BET) analysis, differential scanning calorimetry (DSC), X-ray diffraction (XRD) studies, and thermogravimetric analysis (TGA).

Experimental
Materials and methods
The Bile salt and Proteose peptone were procured from Hi Media Laboratories Pvt. Ltd., Mumbai, India. Each material was divided into two parts; one was kept as a control sample, while the other was subjected to Mr. Trivedi's biofield treatment and coded as treated sample (T). The treatment group (T) was in sealed pack and handed over to Mr. Trivedi for biofield treatment under laboratory condition. Mr. Trivedi provided the treatment through his energy transmission process to the treated group without touching the sample.
Characterization
Particle size analysis: The average particle size and particle size distribution were analyzed by using Sympetac Helos-BF Laser Particle Size Analyzer with a detection range of 0.1 micrometer to 875 micrometer. Average particle size d50 and d99 (size exhibited by 99% of powder particles) were computed from laser diffraction data table. The d50 and d99 values were calculated by the following formula:

Percentage change in d50 size=100 × (d50 treated-d50 control)/d50 control

Percentage change in d99 size=100 × (d99 treated-d99 control)/d99 control

Surface area analysis: The surface area of BS and PP were characterized using surface area analyzer, SMART SORB 90 BET, which had a detection range of 0.1-100 m2/g.

Differential scanning calorimetry (DSC) study: The control and treated samples (BS and PP) were analyzed using a Pyris-6 Perkin Elmer DSC on a heating rate of 10°C/min under air atmosphere.

X-ray diffraction (XRD) study: XRD of BS and PP (control and treated) powders were analyzed by using Phillips Holland PW 1710 X-ray diffractometer system. The wavelength of the radiation was 1.54056 angstrom. The data was obtained in the form of 2θ versus intensity (a.u) chart. The obtained data was used for calculation of crystallite size using the following formula.

Crystallite size=kλ/b Cos θ

Where λ is the wavelength and k is the equipment constant (0.94).

Thermogravimetric analysis-Differential thermal analysis (TGA-DTA): Thermal stability of control and treated samples (BS and PP) were analyzed using Mettler Toledo simultaneous TGA and Differential thermal analyzer (DTA). The samples were heated from room temperature to 400°C with a heating rate of 5°C/min under air atmosphere.

Results and Discussion
Particle size and surface area analysis
The average particle size (d50) and particle size (d99) of the organic products were computed from particle size distribution graph and the data are presented in Figures 1 and 2. The average particle size (d50) of treated BS (13.24 μm) was enhanced as compared to control sample (12.13 μm) ( Figure 1). Similarly, the d99 value of the treated BS (121.65 μm) showed increase as compared to control sample (88.77 μm). The calculated percentage change in average particle size (d50) was 9.2% and d99 value was 37%. This substantial increase in particle size of the treated BS may be due to biofield treatment which may be caused fracture in the particles hence the powder may not have specific boundaries that can be led to particles agglomeration and increased particle size. It is assumed that bigger treated microparticles may be useful in designing drug delivery systems. Many reports suggested that higher water uptake of organic products such as rice bran, mainly depends on its particle size [ 26- 28]. It was envisaged that larger size particles take up more water as compared to smaller particles [ 29]. Albers et al., in a research study showed that water absorption decreased with decreasing particle size [ 26]. Hence, bigger microparticles are likely to show more water uptake and this property can be used for controlled release of drugs. The drugs can be released from swellable microparticle through diffusion, degradation or both depending on the level of swelling and solubility of the drug [ 30]. Therefore, the treated BS could be interesting choice for drug delivery systems.

The PP also showed increase in d50 (14.4 μm) and d99 (130.09 μm) as compared to control PP sample (d50; 13.42, d99; 107.58) ( Figure 1). The percentage change in d50 and d99 values was 7.3% and 20.9 % respectively ( Figure 2). It is assumed that aggregation due to biofield treatment might cause the particles to come together and form bigger microparticles. It was previously described that proteins have stronger tendency of forming aggregates. They can form self-aggregates in a number of ways such as formation of structural complexes, multimeric native states with metal complexation [ 31- 33]. These proteins have sufficiently strong inter-protein interactions [ 34] which induce formation of bigger aggregates. It is postulated that biofield may be interacted with protein assembly of PP and caused bigger microparticle formation.

Surface area of BS and PP was measured and results are presented in Table 1. The surface area of control BS was 0.63 m2/g. However, after treatment it was decreased slightly i.e. up to 0.62 m2/g. The percentage decrease in surface area was by 1.59% in treated BS sample as compared to control. The minimal decrease in surface area was due to increase in particle size of treated BS [ 35, 36]. Contrarily, the treated PP (1.08 m2/g) showed increase in surface area as compared to control (1.00 m2/g). The percentage increase in surface area was 8% in the treated PP with respect to control. It is assumed that biofield energy might cause formation of sharp edges or pore formation over particle surface which increased the resultant surface area.

Table 1: Surface area analysis of bile salt and proteose peptone.
DSC studies
DSC thermogram of control and treated BS are presented in Figure 3. The DSC thermogram of control BS showed broad endothermic peak at 128°C which was due to melting temperature of the sample. DSC of treated BS showed endothermic peak at 232°C which was probably associated with melting and hydration of the hydrophilic head in BS structure. The increase in melting temperature may be correlated with high thermal stability of treated BS. In BS the increase in temperature raises the critical micelle concentration; however further increase in temperature decreases the critical micelle concentration. At a fixed temperature the critical micelle concentration value of BS is controlled by balanced interaction of two forces namely van der Waals forces between the hydrophobic alkyl groups that stabilizes the micelles and opposing hydration of hydrophilic group that deny the formation of micelles. These two opposite factors are to be considered in order to understand this behavior, viz increase in temperature elevates the dehydration of head group (resulting in increased hydrophobic nature of the molecules) and thermal stability of the BS molecules [ 37]. Hence, it is postulated that thermal along with biofield energy might be acted at BS molecules which enhanced the hydrophobic nature and thermal stability.

DSC thermogram of control and treated PP are shown in Figure 4. The control PP showed an endothermic peak due to absorbed water at 122°C and another endothermic inflexion was observed at around 300°C which was due to melting temperature and thermal denaturation of the control sample. DSC thermogram of treated sample exhibited a broad endothermic peak at 175°C. Researchers have found that the major endothermic peak observed (from 0 to 180°C) in case of soy protein, gelatin, sodium casein and corn gluten meal has been attributed to loss of residual water or hydrogen bond disruption between protein molecules [ 38- 41]. Another endothermic peak was observed at 290°C in the treated PP which was probably due to thermal denaturation and melting temperature of the protein. The control PP showed an exothermic temperature peak at around 267°C. However, the DSC thermogram of treated PP showed ( Figure 4) an exothermic transition at 278°C. Tang et al. during their studies on heat induced aggregation and denaturation of soy proteins observed much lower exothermic peaks (150°C) [ 38]. It is presumed that exothermic peak might be increased due to biofield treatment. The native confirmation of proteins is mainly maintained by its inherent hydrogen bonding and electrostatic interactions, whereas thermal stability is closely related to hydrophobic interactions. If the hydrophilic interactions retaining the tertiary structure of protein are ruptured by heating, hydrophobic regions initially hidden inside the proteins will be exposed to the protein surface and subordinate with other hydrophobic protein molecules to form aggregates. Hence, in this study the exothermic peak could be attributed to the protein aggregation [ 42, 43]. Additionally, the increase in exothermic peak may be due biofield energy which raised the treated PP aggregation temperature.
XRD studies
XRD diffractogram of control and treated BS are illustrated in Figure 5. The XRD diffractogram of control BS showed presence of broad as well as intense peak. The control sample showed crystalline peaks at 2θ equals to 31.61°, 45.40°, 56.42°, 66.12° and 75.26°. The control BS showed a broad peak at 2θ equals to 11.1° which was due to amorphous nature. These XRD peaks showed presence of both crystalline as well as amorphous regions in the control BS ( Figure 5). The treated BS also displayed similar XRD peaks at 2θ equals to 27.2°, 31.63°, 45.39°, 56.45°, 66.14° and 75.10°. The result showed a minimal reduction in the intensity of the XRD peaks in the treated BS which may be due to decrease in crystallinity of the sample.

XRD diffractogram of control and treated PP are shown in Figure 6. The XRD of control sample showed an amorphous nature which was confirmed by a broad peak at around 2θ equals to 14.48° and 23°. However, the treated PP showed ( Figure 6) XRD peaks at around 2θ equals to 28.39° and 32.71° which showed no significant change in amorphous nature of the treated PP after biofield treatment.
TGA studies
TGA was used to get further insights about the thermal stability of the control and treated samples (BS and PP). The TGA thermogram of BS (control and treated) are presented in Figures 7 and 8. The TGA thermogram of control BS exhibited one step thermal degradation. The thermal degradation commenced at 80°C and continued up to 130°C. The sample had lost 3.28% of its total original weight during this process. This thermal event was probably associated with elimination of water or dehydration of the control sample ( Figure 7). The derivative thermogravimetry (DTG) thermogram of control sample exhibited maximum thermal degradation at 106°C. Whereas the treated BS sample showed two step thermal degradation process. The first step commenced at around 190°C and terminated at around 240°C. The second thermal degradation event was commenced at 320°C and terminated at around 390°C. Major sample weight loss was observed during this process (15.87%). DTG thermogram of treated BS showed ( Figure 8) maximum thermal decomposition step at 221°C which was higher as compared to control sample (106°C). The DTA thermogram of control and treated BS did not show any changes in the respective thermograms. However, the comparison of the DTG peaks confirmed that thermal stability of treated BS was enhanced after biofield treatment as compared to control sample [ 37].

TGA thermogram of control and treated PP are depicted in Figures 9 and 10. TGA thermogram of control PP showed two step thermal degradation. The first step thermal degradation started at 190°C and terminated at 230°C. However the second thermal degradation commenced at 260°C and terminated at 310°C ( Figure 9). The control sample lost -9.15% and -9.84% weight respectively from the sample. DTG thermogram showed two peaks which were mainly due to initial decomposition temperature (205°C) and maximum thermal decomposition temperature (283°C) of the control sample. The TGA thermogram of treated PP also showed two step thermal degradation pattern. The first step thermal degradation started at 172°C and terminated at 259°C. Thereafter the second step was observed at 273°C and thermal degradation stopped at 371°C. During this thermal process the treated sample lost -18.28% and -21.70% of its weight. The DTG of treated PP showed ( Figure 10) a decrease in maximum thermal decomposition temperature and it was observed at 217°C. This decrease in DTG peak could be due to decrease in thermal stability of treated PP as compared to control. DTA showed no changes in the thermal pattern of control and treated PP.

Conclusion

This research study has evaluated the influence of biofield treatment on thermal and physical properties of BS and PP. The treated BS showed increase in particle size (d50 and d99) as compared to control which might be due to fracturing of internal boundaries in the particles caused by biofield treatment. The treated PP also showed increase in particle size with respect to control; possibly due to biofield treatment and protein aggregation. Additionally, biofield treatment showed significant alteration in thermal nature of the treated samples. Based on the results the biofield treated BS and PP could be used in drug delivery systems. Further, Circular dichroism spectroscopic and Scanning electron microscopy/Transmission electron microscopy studies could be carried out to get further depth insights about thermal stability and aggregation nature of these treated organic products.

Acknowledgements

The authors would like to thank all the laboratory staff of MGV Pharmacy College, Nashik for their assistance during the various instrument characterizations. We thank Dr. Cheng Dong of NLSC, institute of physics, and Chinese academy of sciences for permitting us to use PowderX software for analyzing XRD results.

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# data[].attributes.attachment_link.keywords[]
bile salt; proteose peptone; particle size; brunauer-
emmett-teller analysis; x-ray diffraction; differential scanning
calorimetry

# data[].attributes.attachment_link.title
Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone

# data[].attributes.attachment_link.description
1 Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA 2 Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India *Corresponding Author: Snehasis Jana Trivedi Science Research Laboratory Pvt.

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		Improve the Quality of Beef Extract
	

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		Improve the Quality of Beef Extract
	

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| The present research work investigated the influence of biofield treatment on two common flavoring agents used
in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). For more info visit on www.trivediscience.com.

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		 Characteristics of Klebsiella Pneumoniae: Impact of Biofield
	

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		 Characteristics of Klebsiella Pneumoniae: Impact of Biofield
	

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| The study shows that due to the influence of energy treatment there was a significant impact on antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug-resistant isolates of K. Pneumoniae.

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Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Mayank Gangwar | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

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	 Klebsiella Pneumoniae Biochemical Analysis
	

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	 Klebsiella Pneumoniae Biochemical Analysis
	

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| Klebsiella Pneumoniae Biochemical test results of both control and healing energy treated groups are summarized in the present research paper. Visit to know more about the research and results.

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Peer Evaluation : Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

# data[].attributes.attachment_link.description
Abstract : Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae)and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat inpatients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatmenton four MDR clinical lab isolates (LS) of K.

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	 Klebsiella Pneumoniae Characteristics | Effects of External Energy
	

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	 Klebsiella Pneumoniae Characteristics | Effects of External Energy
	

# data[].attributes.body
| The result of the present work showed a significant impact of external energy treatment on antimicrobial susceptibility pattern, MIC values, biochemical reactions, and biotype number in all the four clinical MDR lab isolates of K. pneumoniae.

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"Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

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		Klebsiella Pneumoniae Biochemical Characteristics Assessment
	

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		Klebsiella Pneumoniae Biochemical Characteristics Assessment
	

# data[].attributes.body
| The Klebsiella Pneumoniae biochemical characteristics were observed to assess the human energy effects on MDR of Klebsiella Pneumoniae and significant positive changes were noticed.

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	 Klebsiella Pneumoniae Antibiogram Typing
	

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	 Klebsiella Pneumoniae Antibiogram Typing
	

# data[].attributes.body
| A significant change in antibiogram typing of Klebsiella Pneumoniae was reported in all four clinical isolates of MDR K. pneumoniae after biofield treatment as compared to control group.

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care

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		 A Research on Characteristics of Klebsiella Pneumoniae
	

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		 A Research on Characteristics of Klebsiella Pneumoniae
	

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| The aim of the present study was to evaluate the impact of unique human energy transmission on various biochemical characteristics of K. Pneumoniae. Read here for more details.

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		Klebsiella Pneumoniae Biochemical Tests
	

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		Klebsiella Pneumoniae Biochemical Tests
	

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| Based on the biochemical test results of Klebsiella Pneumoniae, significant alteration in biotype numbers were observed in all the human energy treated lab isolates as compared to control.

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		 Biofield Impact on Klebsiella Pneumoniae Characteristics
	

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		 Biofield Impact on Klebsiella Pneumoniae Characteristics
	

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| This study evaluates the impact of biofield treatment on biochemical characteristics of Klebsiella Pneumoniae characteristics such as biochemical reactions, biotype numbers and etc.

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Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

Research ArticleOpen Access

Trivedi et al.,J Trop Dis 2015, 3:4

http://dx.doi.org/10.4173/2329-891X.1000173

Research Article Open Access

Journal of Tropical Diseases

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Harish Shettigar1, Mayank Gangwar2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Citation: Trivedi MK, Branton A, Trivedi D, Shettigar H, Gangwar M, et al. (2015)

Antibiogram Typing and Biochemical Characterization of Klebsiella Pneumonia

after Bioeld Treatment. J Trop Dis 3: 173. doi:10.4173/2329891X.1000173

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Keywords: Klebsiella pneumoniae; Bioeld Treatment; Multidrug-

Resistant; Antimicrobial susceptibility; Biochemical Reaction;

shaped, facultative anaerobic, and nonmotile bacterium, belongs to

family Enterobacteriaceae . It is a common human pathogen associated

with nosocomial and community infections [1]. K. pneumoniae isolates

causes several infections such as pneumonia, septicemia, wound

infections, and urinary tract infections, which ultimately lead to

morbidity and mortality especially in immunocompromised patients,

and patients of intensive care units, pediatrics and surgical wards [2].

K. pneumoniae acquire resistance against existing antimicrobials by

multiple mechanism results in increased multidrug-resistant (MDR)

of K. pneumoniae thatleads to serious problem in hospital settings

and health concern. Emergence of resistance occurs not only in

MDR isolates but also exist in pan-drug resistant (PDR) isolates of K.

pneumoniae. PDR refers to the resistant strains those are specically

resistant to 7 antimicrobial agents such as cefepime, imipenem,

meropenem, ceazidime, ciprooxacin, piperacillin-tazobactam,

and levooxacin [3]. Apart from this, the extended-spectrum

β-lactamase (ESBL) producing Klebsiella from a patient has been

identied which causes serious threat worldwide [4,5]. Continuous

use of antibiotics leads to resistance in microorganisms via. dierent

pathways mediated by plasmids, transposons, and gene cassettes in

integrons [6,7]. Carbapenem is usually preferred for the infection

caused by MDRisolates of K. pneumoniae but recently carbapenem-

resistant K. pneumoniae was also reported [8]. Due to dramatically

increase in drug resistant in K. pneumoniae, very few treatment

options are available. Alternative approaches are available but altering

the sensitivity pattern of antimicrobials using bioeld is not available

against MDR microorganism, apart from existing allopathic system of

medicine. Bioeld treatment may be an alternative approach to alter

the susceptibility pattern of K. pneumoniae. Complementary and

alternative medicine (CAM) therapies are commonly practiced in

healthcare sector and about 36% of Americans regularly uses some form

of CAM [9]. CAM include numerous energy therapies, bioeld therapy,

is one of the energy medicine widely used worldwide to improve the

human health. e energy exists in various forms that can be produced

from dierent sources such as potential, electrical, kinetic, magnetic,

and nuclear energy. However, electromagnetic eld denes as when

electrical signals uctuate will generate magnetic eld with respect

to time. e cumulative eect of bio-magnetic and electric eld that

surrounds the human body is dened as bioeld. e bioeld energy can

be monitored by using electromyography (EMG), electrocardiography

(ECG) and electroencephalogram (EEG) [10]. According to Lucchetti

et al. bioeld energy has shown signicant eect on growth of bacterial

cultures [11]. Mr. Trivedi has the ability to harness the energy from

environment or universe and can transmit into any living or nonliving

object(s) around the Universe. e objects always receive the energy

and responding into useful way via bioeld energy and the process is

known as bioeld treatment. Mr. Trivedi's unique bioeld treatment is

extensively studied in dierent elds such as in material science [12,13],

agricultural science [14-16], and in biotechnology [17]. Further,

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae)

patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's bioeld treatment

on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into

two groups i.e. control and bioeld treated. Control and treated groups were analyzed for antimicrobial susceptibility

pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away®

system. The analysis was done on day 10 after bioeld treatment as compared with control group. Antimicrobial

sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of

treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was

reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in

biochemical reactions as compared to control. A signicant change in biotype numbers were reported in all four clinical

number after bioeld treatment, new organism was identied as Enterobacter aerogenes in LS 2 and LS 14. These

results suggest that bioeld treatment has a signicant effect on altering the antimicrobial sensitivity, MIC values,

biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

the bioeld treatment has considerably altered the susceptibility of

antimicrobials and biotype of microbes [18-20]. By considering the

above mentioned facts and literature reports on bioeld treatment,

the present work was undertaken to evaluate the impact of bioeld

treatment on antimicrobials susceptibility, biochemical reactions

pattern, and biotype of MDR isolates of K. pneumoniae.

Experimental design and bacterial isolates

MDR clinical lab isolates (i.e. LS 2, LS 6, LS 7 and LS 14) of K.

pneumoniae were obtained from stored stock cultures in Microbiology

Lab, Hinduja Hospital, Mumbai. Each MDR strains was divided

into two groups i.e. control and treatment. e acceptability of the

identication media and antimicrobial agents were checked prior to the

study on microorganisms. e antimicrobial susceptibility, biochemical

reactions, and biotype number were evaluated on MicroScan Walk-

Away® (Dade Behring Inc., West Sacramento, CA) using Negative

Breakpoint Combo 30 (NBPC 30) panel. e NBPC 30 panel was stored

at 2 to -25ºC. All antimicrobials and biochemicals were procured from

Bioeld treatment strategy

Treatment groups of each strain, in sealed pack were handed over

to Mr. Trivedi for bioeld treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated groups without touching the samples. e bioeld treated

samples were returned in the similar sealed condition and analyzed

on day 10 using the standard protocols. e study was conducted on

automated MicroScan Walk-Away® system (Dade Behring Inc., USA).

Evaluation of antimicrobial susceptibility assay

Antimicrobial susceptibility patterns of MDR lab isolates of K.

pneumoniae were studied using MicroScan Walk-Away® using Negative

Break Point Combo (NBPC 30) panel as per manufacturer's instructions.

e antimicrobial susceptibility pattern (S: Susceptible, I: Intermediate,

IB: Inducible β-lactamase; EBL: Suspected extended-spectrum β

-lactamases, and R: Resistant) and MIC values were determined by

observing the lowest antimicrobial concentration showing growth

inhibition [21]. e antimicrobials used in the susceptibility assay viz.

amikacin, amoxicillin/k-clavulanate, ampicillin/sulbactam, ampicillin,

aztreonam, cefazolin, cefepime, cefotaxime, cefotetan, cefoxitin,

ceazidime, ceriaxone, cefuroxime, cephalothin, chloramphenicol,

ciprooxacin, gatioxacin, gentamicin, imipenem, levooxacin,

meropenem, moxioxacin, noroxacin, nitrofurantoin piperacillin,

piperacillin/tazobactam, tetracycline, ticarcillin/K-clavulanate,

tobramycin, and trimethoprim/sulfamethoxazole

Biochemical studies of each MDR isolates of K. pneumoniae were

determined by MicroScan Walk-Away® using NBPC 30 panel system

in both control and treated groups. Biochemicals used in the study

are acetamide, adonitol, arabinose, arginine, cetrimide, cephalothin,

citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose, hydrogen

sulde, indole, inositol, kanamycin, lysine, malonate, melibiose, nitrate,

oxidation-fermentation, galactosidase, ornithine, oxidase, penicillin,

ranose, rhamnose, sorbitol, sucrose, tartarate, tryptophan deaminase,

tobramycin, urea, and Voges-Proskauer [21].

Identication by biotype number

e biotype number of each MDR isolates of K. pneumoniae in

control and treated sample were determined followed by identication

of microorganism by MicroScan Walk-Away® processed panel data

report with the help of biochemical reaction data [21].

Antimicrobial susceptibility study

Results of antimicrobial sensitivity pattern and MIC values of

control and treated MDR isolates of K. pneumoniae are summarized

in Tables 1 and 2, respectively. All these changes were observed on 10

days aer bioeld treatment as compared to control group. Overall,

46.42% of tested antimicrobials out of twenty eight, showed alteration

in antimicrobial sensitivity pattern against bioeld treated MDR

isolates of K. pneumoniae. All four MDR isolates, showed variations

in antimicrobial sensitivity assay viz. 32.14% in LS 2, 25% in LS 6,

17.85% in LS 7, and 28.57% in LS 14 against the tested antimicrobials

(Figure 1). Extended spectrum beta-lactamases (ESBLs) are rapidly

evolved group of beta-lactamases enzyme, which confer resistance to

most beta-lactam antibiotics, including penicillins, cephalosporins,

monobactam and aztreonam. Apart from beta-lactam antibiotics,

ESBLs are also resistant to other classes of non-penicillin antibiotics

[22]. Beta-lactamases are enzymes that inactivates the antibiotic and are

present in almost all Gram-negative bacilli. However, some pathogenic

production of β-lactamase which varies from low to high level. In

some species, exposure to β-lactams will induced the production level

of β-lactamase, commonly results in resistance to these agents. ese

inducible β-lactamases are frequently found in Enterobacter spp. [23].

Experimental results of antimicrobial sensitivity assay showed altered

sensitivity pattern in bioeld treated clinical isolates of K. pneumonia .

Aztreonam, cefotaxime, ceazidime, and ceriaxone sensitivity

changed from EBL → R in LS 2 and LS 14. Sensitivity of amoxicillin/k-

clavulanate changed from S → R in LS 7 and LS 14, while S → IB and

I → R in LS 2 and LS 6 respectively. Cefotetan and cefoxitin found

changed sensitivity pattern from S → R in LS 7, and while S → IB in

LS 2. Sensitivity of cefotetan changed from I → R in LS 6 while S →

IB in LS 14. Chloramphenicol and imipenem sensitivity changed from

S → R in LS 6. Although, in imipenem sensitivity changed from S →

I in LS 6. Meropenem sensitivity changed from I → R and S → R in

LS 6 and LS 7 respectively. An increase in sensitivity was reported in

piperacillin/tazobactam and piperacillin i.e. from R → I in bioeld

treated LS 6 as compared to control. Although, piperacillin/tazobactam

sensitivity changed from S → IB and S → I in LS 2 and LS 14 respectively.

Ticarcillin/K-clavulanate sensitivity altered from S → I and S → R in LS

2 and LS 14 respectively (Table 1). Rest of antimicrobials did not show

any change in sensitivity pattern aer bioeld treatment.

Determination of Minimum Inhibitory Concentration (MIC)

MIC values of all the clinical MDR isolates of control and bioeld

treated K. pneumoniae are summarized in Table 2. MIC values were

decreased in case of piperacillin/tazobactam and piperacillin in LS

6 isolate only, while in rest of the antimicrobials, MIC values were

increased as compared to control. Overall, MIC results showed an

alteration in 30% tested antimicrobials (i.e. nine out of thirty) aer

bioeld treatment in clinical isolates of K. pneumoniae (Table 2 and

Figure 1). A decreased in MIC values in piperacillin/tazobactam and

piperacillin were reported along with increases antimicrobial sensitivity

aer bioeld treatment (64 µg/ml) in LS 6. Current treatment strategy

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

S. No.AntimicrobialLS 2LS 6LS 7LS 14

8 CefotaximeEBLREBLEBL EBLEBLEBLR

C: Control; T: Treatment; R: Resistant; I: Intermediate; S: Susceptible; LS: Lab Isolate; EBL: Suspected extended-spectrum beta-lactamases

against K. pneumoniae infections preferably uses cefoperazone/

sulbactam, piperacillin/tazobactam, and imipenem antimicrobials

[24]. Although, piperacillin/tazobactam antimicrobial agent is useful

and preferred in neonatal infections caused due to K. pneumoniae [25].

Bioeld treatment in clinical isolate (LS 6) signicantly increased the

sensitivity and decreased the MIC values of piperacillin/tazobactam

and piperacillin. In Enterobacteriaceae family, the most prevalent

mechanism of acquired resistance in β-lactam antibiotics (piperacillin/

tazobactam and piperacillin) are the production of β-lactamases [26].

Bioeld treatment might act on enzymatic or genetic level which might

aects the β-lactamases production that may lead to alter the sensitivity

pattern of tested antimicrobials.

Biochemical and biotype number study

Biochemical study results of control and bioeld treated groups

are summarized in Table 3 and Figure 1. Results showed that overall

15.15% change in tested biochemical reactions among 4 treated MDR

clinical isolates of K. pneumoniae as compared to control. Cetrimide

changed from (-) negative to (+) positive reaction in LS 6, LS 7, and LS

14 as compared to control. Voges-Proskauer changed from (+) positive

to (-) negative reaction in LS 2, LS 6, and LS 7. Indole changed from

(+) positive to (-) negative reaction in LS 7. Ornithine changed from

negative (-) to positive (+) reaction in LS 14. Rest of biochemicals did

not show any alteration in their reaction aer bioeld treatment. Voges-

Proskauer, citrate, arabinose, lysine, glucose, sucrose, malonate are the

standard positive reaction biochemical tests of K. pneumoniae while

hydrogen sulde, indole, ornithine and cetrimide are the standard

negative reaction test. Biochemical reactions of control MDR isolates

of K. pneumoniae were well supported with literature data [27]. Based

on the biochemical results, signicant alteration in biotype numbers

were observed in all the four bioeld treated lab isolates i.e. LS 2, LS 6,

LS 7, and LS 14 as compared to control. New organism was identied

as Enterobacter aerogenes in LS 2 and LS 14 aer bioeld treatment

on day 10 with respect to control (Table 4). Bioeld treatment as

an alternate and complementary medicine, increasingly used in

biomedical health care system such as reduction in pain and anxiety

[28]. However, National Center for Complementary and Alternative

Medicine/National Institute of Health (NCCAM/NIH), now dened

bioeld therapies in subcategory of energy therapies as one of the

ve complementary medicine domain [29]. Mr. Trivedi's bioeld

treatment in pathogenic microbes were extensively studied and had

shown signicant alteration in the antimicrobial sensitivity pattern,

biochemical reactions, and biotype number [18,19]. Bioeld treatment

might be responsible to do alteration in microorganism at genetic level

and/or enzymatic level, which may act on receptor protein. While

altering receptor protein, ligand-receptor/protein interactions may

also alter that could lead to show dierent phenotypic characteristics.

Hence a cascade of intra-cellular signals may be initiated, accelerated or

inhibited [30]. e overall observations showed that, bioeld treatment

on MDR isolates of K. pneumoniae induced signicant alteration in

antimicrobial susceptibility pattern, MIC values, biochemical reactions,

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

# data[].attributes.headline
	
	
	
	
	
		Physical, Thermal & Structural Properties of Chromium Vi Oxide
	

# data[].attributes.subject
	
	
	
	
	
		Physical, Thermal & Structural Properties of Chromium Vi Oxide
	

# data[].attributes.body
| The main objective of this study was to evaluate the impact of unique energy treatment on physical, thermal and structural properties of Chromium Vi Oxide Powder.

# data[].attributes.attachment_link.title
Physical, Thermal and Structural Properties of Chromium (vi) Oxide Powder:Impact of Biofield Treatment

# data[].attributes.attachment_link.description
The objective of the present study was to analyse the alterations in physical, thermal and structural properties of Chromium VI Oxide powder under the influen...

# data[].attributes.headline
	
	
	
	
	
		Variation in Spectral Properties of Pharmaceuticals Compound
	

# data[].attributes.subject
	
	
	
	
	
		Variation in Spectral Properties of Pharmaceuticals Compound
	

# data[].attributes.body
| According to the study, there is an impact of biofield on physical & spectral properties of pharmaceuticals compound like Urea, Thiourea, Sodium carbonate, Magnesium sulphate. Visit the link for more details.

# data[].attributes.attachment_link.title
An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds | Mahendra Trivedi | Shrikant Patil | Harish Shettigar | Ragini Singh | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness. The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis.

# data[].attributes.headline
	
	
	
	
	 Magnesium Sulphate FT-IR Analysis- Energy Treatment Assessment
	

# data[].attributes.subject
	
	
	
	
	 Magnesium Sulphate FT-IR Analysis- Energy Treatment Assessment
	

# data[].attributes.body
| Magnesium sulphate or Epsom salt used as both externally & internally pharmaceutical preparation. In the present study, the FT-IR analysis of Magnesium sulphate after the biofield treatment shows changes in various spectral properties.

# data[].attributes.attachment_link.title
Peer Evaluation : An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds

# data[].attributes.attachment_link.description
Abstract : The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness.The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis.

# data[].attributes.headline
	
	
	
	
	
		 Spectroscopic Characterization of Pharmaceuticals Compound
	

# data[].attributes.subject
	
	
	
	
	
		 Spectroscopic Characterization of Pharmaceuticals Compound
	

# data[].attributes.body
| The spectroscopic characterization of pharmaceuticals compounds (Urea, Thiourea, Sodium Carbonate & Magnesium Sulphate) shows significant changes in spectral properties due to the impact of human energy treatment.

# data[].attributes.attachment_link.title
"An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness. The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis.

# data[].attributes.headline
	
	
	
	
	
		 Alteration in IR Spectra of Thiourea
	

# data[].attributes.subject
	
	
	
	
	
		 Alteration in IR Spectra of Thiourea
	

# data[].attributes.body
| Human energy treatment influences the spectroscopy properties of pharmaceutical compounds i.e. IR spectra of thiourea shows the changes in frequency as compared to control, after using the FTIR and UV-Vis analysis techniques.

# data[].attributes.headline
	
	
	
	
	
		Sodium Carbonate FT-IR Spectrum
	

# data[].attributes.subject
	
	
	
	
	
		Sodium Carbonate FT-IR Spectrum
	

# data[].attributes.body
| The study was undertaken to determine the changes in sodium carbonate’ spectral and physical properties by studying the IR spectrum found in FT-IR analysis of biofield treated samples.

# data[].attributes.attachment_link.title
An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds

# data[].attributes.attachment_link.description
The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness. The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis.

# data[].attributes.headline
	
	
	
	
	
		 FT-IR Spectrum Analysis of Pharmaceuticals Compounds
	

# data[].attributes.subject
	
	
	
	
	
		 FT-IR Spectrum Analysis of Pharmaceuticals Compounds
	

# data[].attributes.body
| Spectrum Analysis of Pharmaceuticals compound was used to determine the human energy changes in physical and structural properties of tested samples using the analysis techniques like FT-IR & UV-Vis. Read here for more details.

# data[].attributes.attachment_link.title
An Impact of Biofield Treatment on Spectroscopic Characterization of Pharmaceutical Compounds

# data[].attributes.attachment_link.description
The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness. The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be

# data[].attributes.headline
	
	
	
	
	 Analysis of Thiourea IR Spectrum
	

# data[].attributes.subject
	
	
	
	
	 Analysis of Thiourea IR Spectrum
	

# data[].attributes.body
| Thiourea IR spectrum was analyzed using the FT-IR and UV-Vis spectroscopic analysis technique and noticed the changes in different spectral properties of tasted pharmaceutical compounds.

# data[].attributes.headline
	
	
	
	
	
		Evaluation of Spectral Properties of Pharmaceuticals Compound
	

# data[].attributes.subject
	
	
	
	
	
		Evaluation of Spectral Properties of Pharmaceuticals Compound
	

# data[].attributes.body
| The present study was aimed to evaluate the impact of biofield energy treatment on spectral properties of pharmaceutical compounds such as urea, thiourea, sodium carbonate, and magnesium sulphate, using spectroscopic analysis.

# data[].attributes.headline
	
	
	
	
	 Changes in Characteristics of Meat Infusion Powder
	

# data[].attributes.subject
	
	
	
	
	 Changes in Characteristics of Meat Infusion Powder
	

# data[].attributes.body
| The Energy medicine could be used to bring changes in various physiochemical characteristics of Meat Infusion Powder and Beef Extract Powder. The samples were thoroughly characterized by FT-IR, XRD, DSC, TGA, CHNSO, and particle size analysis in this study.

# data[].attributes.attachment_link.title
Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).

# data[].attributes.headline
	
	
	
	
	Changes in Beef Powder Characteristics
	

# data[].attributes.subject
	
	
	
	
	Changes in Beef Powder Characteristics
	

# data[].attributes.body
| The results of this study show significant alterations in Beef Powder Characteristics after unique energy treatment, that could help in improving the quality of beef powder. Read here for more details.

# data[].attributes.attachment_link.title
"Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).

# data[].attributes.headline
	
	
	
	
	



	
	
	
	
	 Improving the Quality of Beef Extract Powder
	





	

# data[].attributes.subject
	
	
	
	
	



	
	
	
	
	 Improving the Quality of Beef Extract Powder
	





	

# data[].attributes.body
| In this study, an attempt was made to investigate the human energy impact on how it could help improving the quality of beef extract and meat infusion powder. Read here to know about the results.

# data[].attributes.attachment_link.title
Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).

# data[].attributes.headline
	
	
	
	
	
		 Improving the Quality of Beef Extract Powder
	

# data[].attributes.subject
	
	
	
	
	
		 Improving the Quality of Beef Extract Powder
	

# data[].attributes.body
| In this study, an attempt was made to investigate the human energy impact on how it could help improving the quality of beef extract and meat infusion powder. Read here to know about the results.

# data[].attributes.attachment_link.title
Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).

# data[].attributes.headline
	
	
	
	
	 Beef Extract XRD Analysis- Evaluation of Energy Medicine
	

# data[].attributes.subject
	
	
	
	
	 Beef Extract XRD Analysis- Evaluation of Energy Medicine
	

# data[].attributes.body
| To analyze the atomic arrangement & crystallite size of BEP under the influence of energy medicine, the XRD analysis of beef extract power was conducted. Read here for the detailed results.

# data[].attributes.attachment_link.title
Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier

# data[].attributes.headline
	
	
	
	
	
		 Standard Quality of Beef Extract- Biofield Impact
	

# data[].attributes.subject
	
	
	
	
	
		 Standard Quality of Beef Extract- Biofield Impact
	

# data[].attributes.body
| As Beef is known for its high food values and a good flavoring agent, the present study was undertaken to improve the quality of Beef Extract Powder under the influence of Biofield energy treatment.

# data[].attributes.headline
	
	
	
	
	 Meat Infusion Powder: Improving the Quality
	

# data[].attributes.subject
	
	
	
	
	 Meat Infusion Powder: Improving the Quality
	

# data[].attributes.body
| The present study illustrates the significance of human energy treatment by evaluating its impact on physiochemical properties of Meat Infusion Powder. Read here for more details.

# data[].attributes.headline
	
	
	
	
	
		 Physicochemical Properties of Beef Extract Powder
	

# data[].attributes.subject
	
	
	
	
	
		 Physicochemical Properties of Beef Extract Powder
	

# data[].attributes.body
| The study shows that the biofield energy treatment has induced some positive changes in various physicochemical properties of beef extract powder. Read here for the more details.

# data[].attributes.attachment_link.readable_text
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Volume 5 * Issue 4 * 1000389

ISSN: 2155-9600 JNFS, an open access journal

Mahendra et al., J Nutr Food Sci 2015, 5:4

http://dx.doi.org/10.4172/2155-9600.1000389

*Corresponding author: Shrikant P, Trivedi Global Inc., 10624 S Eastern

Avenue Suite A-969, Henderson, NV 89052, USA, Tel: 1602-531-5400; E-mail:

Citation:Mahendra KT, Gopal N, Shrikant P, Rama MT, Snehasis J, et al. (2015)

Bio-eld Treatment: An Effective Strategy to Improve the Quality of Beef Extract and

Meat Infusion Powder. J Nutr Food Sci 5: 389. doi:10.4172/2155-9600.1000389

under the terms of the Creative Commons Attribution License, which permits

original author and source are credited.

Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef

Extract and Meat Infusion Powder

Mahendra KT, Gopal N, Shrikant P*, Rama MT, Snehasis J and Rakesh M

Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

The present research work investigated the inuence of bio-eld treatment on two common avoring agents used

in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were

characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis,

surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR

results showed disappearance of triglycerides peaks in both the treated powders as compared to control. XRD

results corroborated the amorphous nature of both control and treated samples. The BEP showed enhanced average

It was assumed that enormous energy was stored in MIP after bio-eld treatment that led to fracture into smaller

particles. The surface area was increased in both the treated powders. DSC result showed signicant increase in

melting temperature, in BEP and MIP, which indicated the higher thermal stability of the samples. However, the

specic heat capacity (∆H) was decreased in both samples, which was probably due to high energy state of the

powders.

Keywords: Beef extract powder; Meat infusion powder; Bio-eld

treatment; Fourier transform infrared spectroscopy; X-ray diraction;

Particle size analysis; Surface area analysis; Dierential scanning

calorimetry; ermo gravimetric analysis

Abbreviations: BEP: Beef Extract Powder; MIP: Meat Infusion

Powder; FT-IR: Fourier Transform Infrared Spectroscopy; XRD: X-Ray

Diraction; DSC: Dierential scanning Calorimetry; TGA: ermo

Gravimetric Analysis; CHD: Coronory Heart Disease; BET: Brunauer-

Emmett-Teller; DTG: Derivative ermo Gravimetry

Beef is known to have excellent nutritional value and it has been

widely consumed in many countries. e prominent reason for this

high food value is its strongest peroxide forming potential due to its

excellent myoglobin and haem levels [1,2]. Beef extract powder (BEP)

is highly concentrated meat stock and has been used in food industry

as a avouring agent in cooking and to prepare broth for drinks [3]. It

has been used since many years as a food additive and taste enhancer

in food technological applications. On the other hand, meat infusion

powder (MIP) has been used as a microbial growth medium and

Coronory heart disease (CHD) is the main cause of death in western

countries. e life style and genetic backgrounds are two important

factors, which aects the mortality in CHD. e elevated level of

triglycerides is one of the main reasons for CHD. e factors such as

obesity, insulin resistance, excessive alcohol consumption, diabetes, and

kidney disease also causes risk of high triglycerides [5]. Moreover, the

red meat such as beef and less dark meat chicken also have the higher

triglycerides. Previously, it was suggested that removing triglycerides

from cooked meat aects the aroma and thus it can aect the quality of

the beef meat [6]. Hence, reducing the triglyceride content will directly

improve the health and it will improve the quality of the meat products.

Currently, no alternative and cost eective approaches are available to

alter the content of triglycerides, but bio-eld treatment may be a new

approach to change the physiochemical properties of powders made

from these meat products.

In physics, energy is a property of objects that can be transmitted

to other objects and changed into dierent forms but neither can be

created or destroyed [7]. According to Einstein's equation (E=mc2 )

the energy and matter are fundamentally related to each other [8].

Nonetheless, the energy is a eld of force which can signicantly

interact with any object at a distance and cause action. Furthermore, the

energy can exists in several forms such as kinetic, potential, electrical,

magnetic, and nuclear. Researchers have shown that short lived

electrical events or action potential exists in several types of animal

cells such as neurons, muscle cells, endocrine cells as well as plant cells.

e human nervous system consist the energy/information in the form

of electrical signals [9,10]. Whenever, these electrical signals uctuate

with time, the magnetic eld generates as per the Ampere-Maxwell

law, and cumulatively known as electromagnetic eld. Hence, the

electromagnetic eld being generated from the human body is known

Mr. Trivedi is known to exert prominent eects on external

surrounding using his unique bio-eld, herein referred as Bio-eld

treatment. Recently, it was investigated that bio-eld treatment

can signicantly change the characteristics of living and non-living

organisms. Subjecting bio-eld treatment on metals and ceramics

caused signicant changes in crystalline, thermal, and atomic

properties [12-19]. It has been recently published that the eect of

bio-eld treatment resulted in signicant improvement of the yield

and quality of various agriculture products [20-23]. e said bio-eld

Citation: Mahendra KT, Gopal N, Shrikant P, Rama MT, Snehasis J, et al. (2015) Bio-eld Treatment: An Effective Strategy to Improve the Quality of

Beef Extract and Meat Infusion Powder. J Nutr Food Sci 5: 389. doi:10.4172/2155-9600.1000389

Volume 5 * Issue 4 * 1000389

ISSN: 2155-9600 JNFS, an open access journal

exposure caused an increase in growth and anatomical characteristics

of an herb Pogostemon cablin that is commonly used in perfumes, in

incense/insect repellents, and alternative medicine [24]. Moreover,

in microbiology, bio-eld treatment has also changed the antibiotic

susceptibility patterns as well as produced biochemical reactions that

induced changes in the characteristics of pathogenic microbes [25-27].

Having inspired by these excellent results, in present study, an

attempt was made to investigate the physicochemical properties of BEP

and MIP that were exposed to the said Bio-eld. e bio-eld treated

powders were thoroughly characterized by FT-IR, XRD, DSC, TGA,

CHNSO, and particle size analysis.

e BEP and MIP were procured from HiMedia Laboratories Pvt

Ltd, India. e samples were grouped into two parts; one was kept

as a control sample, while the remaining part was subjected to Mr.

Trivedi's bio-eld treatment and coded as treated sample. Aer that,

all the samples (control and treated) were characterized with respect

to FT-IR, CHNSO, XRD, particle size analysis, surface area analysis,

Fourier transforms infrared (FT-IR) spectroscopy: e infrared

spectra of BEP and MIP were recorded with FT-IR spectrometer

(Perkin Elmer, USA). IR spectrum was recorded in the range of 4000

CHNSO analysis: e BEP and MIP were analyzed for their

elemental composition (C,H,N,S, and O). e powdered samples were

subjected to CHNSO Analyzer using Model Flash EA 1112 Series,

X-ray diraction (XRD) study: XRD of BEP and MIP were

analyzed using Phillips Holland PW 1710 X-ray diractometer system.

e wavelength of the radiation was 1.54056 angstrom. e data was

obtained in the form of 2θ versus intensity (a.u) chart. e obtained

data was used for calculation of crystallite size using the following

formula.

Crystallite size= kλ/b Cos θ

Where, λ is the wavelength and k is the equipment constant (0.94).

Particle size analysis: e average particle size and particle size

distribution were analyzed using Sympetac Helos-BF Laser Particle

Size Analyzer with a detection range of 0.1 µm to 875µm. Average

calculated using following formula.

Percentage change in d50 size=100 × (d50 treated- d50 control)/ d50

control.

Percentage change in d99 size=100 × (d99 treated- d99 control)/ d99

Surface area analysis: e surface area of BEP and MIP were

characterized using Surface Area Analyzer, SMART SORB 90 BET

(Brunauer-Emmett-Teller), which had a detection range of 0.1-100

Dierential scanning calorimetry (DSC) study: e BEP and

MIP were used for DSC study. e samples were analyzed using a

Pyris-6 Perkin Elmer DSC on a heating rate of 10ºC/min under oxygen

ermo gravimetric analysis (TGA): ermal stability of the BEP

and MIP were analyzed using Metller Toledo simultaneous TGA. e

samples were heated from room temperature to 400ºC with a heating

rate of 5ºC/min under oxygen atmosphere.

e FT-IR spectrum of control and bio-eld treated samples are

illustrated in Figure 1. e IR spectrum of control and BEP showed

(Figure 1) prominent vibration bands at 1760 cm-1 (-C=O) and

1151 cm-1 (-C-O) due to presence of triglycerides peak stretching in

the sample. Other important peaks were observed at 2895 and 2817

cm-1 which can be attributed to C-H stretching vibration peaks. e

spectrum showed peaks at 1635 and 1587 cm-1 attributed to presence of

characteristic protein bands such as amide-I and amide -II stretching

vibration peaks [28-31]. Another peak was observed at 3078 to

3780.2 cm-1 attributed to -OH stretching vibration peak. e treated

sample showed considerable change in FT-IR spectrum (Figure 1).

We observed that the presence of triglycerides peak of -C=O (1760

result showed that the bio-eld treatment, probably removed the fatty

triglycerides components from the treated BEP. Additionally, it was

also observed that the characteristic -OH/-NH stretching vibration

peaks were reduced to lower wavenumbers 3064 cm-1 ,which indicated

the formation of strong intermolecular hydrogen bonding in the

treated sample [32,33]. ese results suggest that bio-eld treatment

has induced structural changes in the treated sample.

e FT-IR spectrum of control and treated MIP are presented in

Figure 2. e FT-IR of control powder showed (Figure 2) important

peaks at 1689 and 1589 cm-1 due to amide-I and amide-II stretching

vibration peaks, respectively. Other important peaks were observed

at 1760 and 1157cm-1 for C=O and C-O group, respectively due to

triglycerides. However, these two peaks were completely disappeared

in treated MIP (Figure 2) which indicated that bio-eld treatment

aected chemical changes in the treated sample.

Figure 1: FTIR spectrum of control and treated beef extract powder.

Citation: Mahendra KT, Gopal N, Shrikant P, Rama MT, Snehasis J, et al. (2015) Bio-eld Treatment: An Effective Strategy to Improve the Quality of

Beef Extract and Meat Infusion Powder. J Nutr Food Sci 5: 389. doi:10.4172/2155-9600.1000389

Volume 5 * Issue 4 * 1000389

ISSN: 2155-9600 JNFS, an open access journal

Table 1 shows the results of CHNSO analysis of BEP and MIP.

e treated BEP showed substantial changes in terms of elemental

composition of the treated sample. e treated BEP showed 5.05%

increase in nitrogen as compared to control. e oxygen percentage

was increased by 3.82% in the treated BEP as compared to control. e

carbon percentage was also improved by 2.09% as compared to control

sample. Moreover, the treated BEP showed the presence of sulphur

element; however no trace of sulphur was found in control sample.

e presence of sulphur might play a crucial role in preserving the

comminuted meat products [34]. is data showed that the bio-eld

treatment led to change the elemental composition in BEP.

Whereas the treated MIP showed small percentage change in

nitrogen (0.77%) as compared to control powder. However, carbon and

hydrogen percentage was decreased by 1.22% and 7.13%, respectively

in the treated sample as compared to control. It was observed that there

was a signicant change in oxygen percentage in treated MIP (24.48%)

as compared to control sample. e treated MIP showed some trace

of sulphur (0.27%) though no sulphur was found in control sample.

All together, the CHNSO results conrmed that bio-eld treatment

signicantly changed the elemental percentage in treated samples.

e XRD diractogram of control and treated BEP sample are

illustrated in Figure 3, where, a and b represented to control and

treated sample respectively. e XRD showed the amorphous nature of

the control sample (Figure 3a) with a broad halo at 2θ equals to 20.0°.

e XRD of treated BEP did not reveal (Figure 3b) any dierences in

X-ray pattern of the sample. e treated samples also showed the broad

amorphous nature which was probably due to less ordered atomic

arrangement in the sample. e X-ray diractogram of control and

treated MIP are presented in Figures 4a and 4b. e Figure 4a showed

a broad amorphous peak at 2θ equals to 22° and Figure 4b showed

similar XRD pattern with no change in peak position. Amorphous

materials due to random or irregular arrangement in atoms show

broad and diused peaks [35].

Particle size and surface area analysis

e particle size analysis was carried out on BEP and MIP. e

results are presented in Figure 5. e control BEP showed d50 value

11.75 μm and d99 value of 85.39 μm respectively. Aer treatment d50

value was increased to 12.42 μm and d99 value was increased to 99.1

BEP was increased by 5.7% and 16.1%, respectively as compared to

control sample (Figure 5). is showed that bio-eld treatment led to

an increase in particle size of the treated samples. It is postulated that

the agglomeration of treated BEP may be due to bio-eld treatment

which causes joining of particle boundaries and hence increase in

0.4% and 18.1% (Figure 6). Here we assume that the treated powder

particles received high bio-eld energy which led to deformation of the

particle boundaries, and hence it caused a reduction in particle size.

e surface area was analyzed by BET analysis and results are

presented in Table 2. e treated BEP showed substantial increase in

surface area (1.291 m2 /g) as compared to control powder (1.027 m2 /g).

is was contrary to our particle size results. e surface area of treated

Figure 2: FTIR spectrum of control and treated meat infusion powder.

Figure 3a: XRD diffractogram of control beef extract powder.

Figure 3b: XRD diffractogram of treated beef extract powder.

# data[].attributes.attachment_link.title
Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder - PdfSR.com

# data[].attributes.attachment_link.description
The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).

# data[].attributes.headline
	
	
	
	
	
		 Thermal Properties of Proteose Peptone – Alterations by Biofield
	

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		 Thermal Properties of Proteose Peptone – Alterations by Biofield
	

# data[].attributes.body
| Biofield treatment showed significant alteration in thermal nature of the treated Proteose Peptone and Bile Salt. This has been observed that the biofield treated BS and PP could be used in drug delivery systems.

# data[].attributes.attachment_link.title
Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone | Mahendra Trivedi | Academic Room

# data[].attributes.headline
	
	
	
	
	
		Physical & Thermal Properties of Proteose Peptone
	

# data[].attributes.subject
	
	
	
	
	
		Physical & Thermal Properties of Proteose Peptone
	

# data[].attributes.body
| Read here how the unique energy treatment has altered the physical and thermal properties of Proteose Peptone and Bile Salt.

# data[].attributes.attachment_link.title
Peer Evaluation : Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone

# data[].attributes.attachment_link.description
Abstract : Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study was performed in two groups (control and treated).

# data[].attributes.headline
	
	
	
	
	
		 Change in Physical Properties of Proteose Peptone
	

# data[].attributes.subject
	
	
	
	
	
		 Change in Physical Properties of Proteose Peptone
	

# data[].attributes.body
| The study shows an alteration in physical properties of Protease peptone and Bile Salt after the energy treatment. Visit the link for more details.

# data[].attributes.attachment_link.title
"Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study was performed in two groups (control and treated).

# data[].attributes.headline
	
	
	
	
	
		 DSC & XRD Analysis of Bile Salt
	

# data[].attributes.subject
	
	
	
	
	
		 DSC & XRD Analysis of Bile Salt
	

# data[].attributes.body
| The main aim of undertaking DSC & XRD analysis of Bile Salt and Proteose Peptone is to identify the changes caused by the spiritual energy treatment on the physical and chemical characteristics of both.

# data[].attributes.headline
	
	
	
	
	
		Thermal & Physial Properties of Bile Salt- Impact of Biofield
	

# data[].attributes.subject
	
	
	
	
	
		Thermal & Physial Properties of Bile Salt- Impact of Biofield
	

# data[].attributes.body
| The study suggest, due to influence of Mahendra Trivedi's biofield energy healing phenomena there is significant changes in the physical & thermal properties of Bile Salt and Proteose Peptone.

# data[].attributes.attachment_link.description
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# data[].attributes.headline
	
	
	
	
	 Alteration in Physical Properties of Bile Salt
	

# data[].attributes.subject
	
	
	
	
	 Alteration in Physical Properties of Bile Salt
	

# data[].attributes.body
| The unique human energy treatment has significantly influenced the physical properties of Bile Salt. The energy treatment has resulted an increase in particle size of Bile Salt.

# data[].attributes.attachment_link.title
Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone

# data[].attributes.attachment_link.description
Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study

# data[].attributes.headline
	
	
	
	
	
		 XRD analysis of Bile Salt to Evaluate Human Energy Impact 
	

# data[].attributes.subject
	
	
	
	
	
		 XRD analysis of Bile Salt to Evaluate Human Energy Impact 
	

# data[].attributes.body
| XRD analysis of Bile Salt and Proteose Peptone was conducted to study the change in physical properties of both after the human energy treatment. Read here to know about the results.

# data[].attributes.headline
	
	
	
	
	 Biofield Impact on Thermal Properties of Bile Salt
	

# data[].attributes.subject
	
	
	
	
	 Biofield Impact on Thermal Properties of Bile Salt
	

# data[].attributes.body
| The results of the present study indicates that the biofield treatment may enhance the thermal stability of treated Bile Salt with respect to untreated samples.

# data[].attributes.headline
	
	
	
	
	
		 Biofield Impact on Physical Properties of Bile Salt
	

# data[].attributes.subject
	
	
	
	
	
		 Biofield Impact on Physical Properties of Bile Salt
	

# data[].attributes.body
| The objective of this study was to investigate the influence of alternative energy treatment on chemical & physical properties of Bile Salt and Proteose Peptone.

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Analytical & Bioanalytical

Trivedi et al.,J Anal Bioanal Tech 2015, 6:4

http://dx.doi.org/10.4172/2155-9872.1000256

Volume 6 * Issue 4 * 1000256

ISSN: 2155-9872 JABT, an open access journal

Keywords: Bile salt; Proteose peptone; Particle size; Brunauer-

Emmett-Teller analysis; X-ray diraction; Dierential scanning

calorimetry; ermogravimetric analysis

Abbreviations: PSA: Particle Size Analyzer; BET: Brunauer-

Emmett-Teller analysis; DSC: Dierential Scanning Calorimetry;

XRD: X-ray Diraction; TGA: ermogravimetric Analysis; DTA:

Dierential ermal Analyzer; DTG: Derivative ermogravimetry;

BS: Bile Salt; PP: Proteose Peptone

Bile salts (BS) are commonly known as bio-surfactants that plays

crucial physiological role in human gastro intestinal tract such as

fat digestion and absorption of nutrients and also serve as a mean

for removal of waste products from blood [1,2]. Briey, BS acts as a

carrier for fat soluble products due to its ability of forming micelles

with phospholipids. Moreover, the BS plays an important role in

nutrition by improving solubility and transport of fat soluble nutrients

to the mucosa of small intestine. Based on chemical nature of BS are

at molecules with both hydrophilic and hydrophobic faces [3]. Many

literature reports provided interesting information about the self-

assembly nature of BS in solution suggesting the fascinating properties

of BS aggregates as compared to conventional surfactants [4-6]. Due to

this micellar nature of BS, which enables solubilization and transport

of lipid soluble compounds thus it helps in fat digestion. erefore,

this same biological function can be exploited for pharmaceutical

application since most drugs currently in development have low water

solubility [1]. us BS based carrier systems are promising for specic

targeting and absorption of non-soluble compounds. However, it

was shown that BS is a poor surface active-agent compared to other

commonly used surfactants such as dodecyl sulfate and sodium

dodecanoate. Hence, in order to improve these properties BS should be

modied in order to confer better physicochemical properties.

On the other hand proteose peptone (PP) is obtained from bovine

milk which is partially consist of a number of heat stable minor proteins,

glycoproteins, and largely of casein derived peptides [7,8]. ese are

generated in human body by the action of proteinases (mainly plasmin)

of all the four main casein proteins [9-11]. ese protein compounds

require proper modication in order to alleviate its properties which

can be utilized for further applications.

Scientists have demonstrated that short lived electrical events or

action potential occurs in several types of mammalian cells such as

neurons, muscle cells, and endocrine cells [12]. For example, the cells

in the nervous system communicate with each another by means of

electrical signals that travel along the nerve processes. erefore,

it is hypothesized that bioeld exists around the human body and

the evidence can be found using medical technologies such as

Electromyography, Electrocardiography and electroencephalogram

us, human has the ability to harness the energy from

environment or universe and can transmit into any living or nonliving

*Corresponding authors: Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026,

Received July 06, 2015; Accepted July 16, 2015; Published July 23, 2015

Citation: Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and Physical

Properties of Bioeld Treated Bile Salt and Proteose Peptone. J Anal Bioanal Tech

6: 256 doi:10.4172/2155-9872.1000256

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human

body. The objective of this study was to investigate the inuence of bioeld treatment on physicochemical properties

of BS and PP. The study was performed in two groups (control and treated). The control group remained as untreated,

and bioeld treatment was given to treated group. The control and treated BS and PP samples were characterized by

particle size analyzer (PSA), Brunauer-Emmett-Teller (BET) analysis, differential scanning calorimetry (DSC), x-ray

diffraction (XRD), and thermogravimetric analysis (TGA). PSA results showed increase in particle size (d50 and d99 )

of both treated BS and PP as compared to control. Surface area analysis showed minimal decrease by 1.59%, in

surface area of treated BS as compared to control. However, the treated PP showed increase (8%) in surface area

as compared to control. DSC characterization showed increase in melting temperature of treated BS as compared to

control. Whereas, DSC thermogram of treated PP showed decrease in melting temperature with respect to control.

Moreover, the DSC of control and treated PP showed presence of exothermic peaks which were possibly due to

protein aggregation. The treated PP showed higher exothermic transition temperature as compared to control. XRD

analysis revealed slight reduction in crystalline nature of BS as compared to control. On the other hand, XRD data

of control and treated PP showed an amorphous nature. TGA analysis of treated BS showed maximum thermal

decomposition temperature at 22°C which was higher as compared to control sample (106°C). This could be due to

bioeld treatment which may enhance the thermal stability of treated BS with respect to control. However, the TGA

thermogram of treated PP showed decrease in maximum thermal stability as compared to control. The overall results

showed that bioeld treatment has signicantly altered the physical and thermal properties of BS and PP.

Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose

Mahendra Kumar Trivedi1, Shrikant Patil1, Rakesh K. Mishra2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

Citation: Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and Physical Properties of Bioeld Treated Bile Salt and Proteose Peptone. J Anal

Bioanal Tech 6: 256 doi:10.4172/2155-9872.1000256

Volume 6 * Issue 4 * 1000256

ISSN: 2155-9872 JABT, an open access journal

objects around the Globe. e objects always receive the energy and

responding into useful way that is called bioeld energy and the process

is known as bioeld treatment. Recently, bioeld energy has shown

signicant eect on structural, crystalline and thermal properties of

various metals, ceramics and carbon allotropes [14-17].

Mr. Mahendra K. Trivedi is known to transform the properties

of various living and non-living things under controlled experiments

using his unique bioeld energy. Bioeld treatment had substantially

changed the atomic, crystalline, surface properties of various materials.

e bioeld had signicantly changed the overall productivity

and quality in the eld of agriculture and biotechnology [18-21].

Additionally, bioeld has shown excellent results in improving

antimicrobial susceptibility, and alteration of biochemical reactions, as

well as induced alterations in characteristics of pathogenic microbes [22-

24]. e bioeld had also caused an increase in growth and anatomical

characteristics of an herb Pogostemon cablin that is commonly used in

perfumes, in incense/insect repellents, and alternative medicine [25].

In the present study, the inuence of bioeld treatment on

physicochemical properties of BS and PP were studied with the aid of

dierent methods like particle size analyzer (PSA), Brunauer-Emmett-

Teller (BET) analysis, dierential scanning calorimetry (DSC), X-ray

diraction (XRD) studies, and thermogravimetric analysis (TGA).

e Bile salt and Proteose peptone were procured from Hi Media

Laboratories Pvt. Ltd., Mumbai, India. Each material was divided

into two parts; one was kept as a control sample, while the other was

subjected to Mr. Trivedi's bioeld treatment and coded as treated

sample (T). e treatment group (T) was in sealed pack and handed

over to Mr. Trivedi for bioeld treatment under laboratory condition.

Mr. Trivedi provided the treatment through his energy transmission

process to the treated group without touching the sample.

Particle size analysis: e average particle size and particle

size distribution were analyzed by using Sympetac Helos-BF Laser

Particle Size Analyzer with a detection range of 0.1 micrometer to 875

micrometer. Average particle size d50 and d99 (size exhibited by 99% of

powder particles) were computed from laser diraction data table. e

d50 and d99 values were calculated by the following formula:

Percentage change in d50 size=100 × (d50 treated-d50 control)/d50

Percentage change in d99 size=100 × (d99 treated-d99 control)/d99

Surface area analysis: e surface area of BS and PP were

characterized using surface area analyzer, SMART SORB 90 BET,

which had a detection range of 0.1-100 m2/g.

Dierential scanning calorimetry (DSC) study: e control and

treated samples (BS and PP) were analyzed using a Pyris-6 Perkin

Elmer DSC on a heating rate of 10°C/min under air atmosphere.

X-ray diraction (XRD) study: XRD of BS and PP (control and

treated) powders were analyzed by using Phillips Holland PW 1710

X-ray diractometer system. e wavelength of the radiation was

1.54056 angstrom. e data was obtained in the form of 2θ versus

intensity (a.u) chart. e obtained data was used for calculation of

crystallite size using the following formula.

Crystallite size=kλ/b Cos θ

Where λ is the wavelength and k is the equipment constant (0.94).

ermogravimetric analysis-Dierential thermal analysis

(TGA-DTA): ermal stability of control and treated samples (BS

and PP) were analyzed using Mettler Toledo simultaneous TGA and

Dierential thermal analyzer (DTA). e samples were heated from

room temperature to 400°C with a heating rate of 5°C/min under air

Particle size and surface area analysis

e average particle size (d50) and particle size (d99) of the organic

products were computed from particle size distribution graph and the

data are presented in Figures 1 and 2. e average particle size (d50) of

treated BS (13.24 µm) was enhanced as compared to control sample

(12.13 µm) (Figure 1). Similarly, the d99 value of the treated BS (121.65

µm) showed increase as compared to control sample (88.77 µm). e

calculated percentage change in average particle size (d50) was 9.2% and

d99 value was 37%. is substantial increase in particle size of the treated

BS may be due to bioeld treatment which may be caused fracture in

the particles hence the powder may not have specic boundaries that

can be led to particles agglomeration and increased particle size. It is

assumed that bigger treated microparticles may be useful in designing

drug delivery systems. Many reports suggested that higher water

uptake of organic products such as rice bran, mainly depends on its

particle size [26-28]. It was envisaged that larger size particles take up

more water as compared to smaller particles [29]. Albers et al., in a

research study showed that water absorption decreased with decreasing

particle size [26]. Hence, bigger microparticles are likely to show more

water uptake and this property can be used for controlled release of

drugs. e drugs can be released from swellable microparticle through

diusion, degradation or both depending on the level of swelling and

solubility of the drug [30]. erefore, the treated BS could be interesting

choice for drug delivery systems.

e PP also showed increase in d50 (14.4 µm) and d99 (130.09 µm)

as compared to control PP sample (d50; 13.42, d99; 107.58) (Figure

1). e percentage change in d50 and d99 values was 7.3% and 20.9 %

respectively (Figure 2). It is assumed that aggregation due to bioeld

treatment might cause the particles to come together and form bigger

Figure 1: Particle size (d50 and d99) distribution of bile salt (BS) and proteose

peptone (PP) (control and treated).

Citation: Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and Physical Properties of Bioeld Treated Bile Salt and Proteose Peptone. J Anal

Bioanal Tech 6: 256 doi:10.4172/2155-9872.1000256

Volume 6 * Issue 4 * 1000256

ISSN: 2155-9872 JABT, an open access journal

microparticles. It was previously described that proteins have stronger

tendency of forming aggregates. ey can form self-aggregates in a

number of ways such as formation of structural complexes, multimeric

native states with metal complexation [31-33]. ese proteins have

suciently strong inter-protein interactions [34] which induce

formation of bigger aggregates. It is postulated that bioeld may be

interacted with protein assembly of PP and caused bigger microparticle

Surface area of BS and PP was measured and results are presented

in Table 1. e surface area of control BS was 0.63 m2/g. However, aer

treatment it was decreased slightly i.e. up to 0.62 m2/g. e percentage

decrease in surface area was by 1.59% in treated BS sample as compared

to control. e minimal decrease in surface area was due to increase

in particle size of treated BS [35,36]. Contrarily, the treated PP (1.08

m2 /g) showed increase in surface area as compared to control (1.00

m2 /g). e percentage increase in surface area was 8% in the treated PP

with respect to control. It is assumed that bioeld energy might cause

formation of sharp edges or pore formation over particle surface which

increased the resultant surface area.

DSC thermogram of control and treated BS are presented in Figure

3. e DSC thermogram of control BS showed broad endothermic peak

at 128°C which was due to melting temperature of the sample. DSC

of treated BS showed endothermic peak at 232°C which was probably

associated with melting and hydration of the hydrophilic head in BS

structure. e increase in melting temperature may be correlated with

high thermal stability of treated BS. In BS the increase in temperature

raises the critical micelle concentration; however further increase in

temperature decreases the critical micelle concentration. At a xed

temperature the critical micelle concentration value of BS is controlled

by balanced interaction of two forces namely van der Waals forces

between the hydrophobic alkyl groups that stabilizes the micelles and

opposing hydration of hydrophilic group that deny the formation

of micelles. ese two opposite factors are to be considered in order

to understand this behavior, viz increase in temperature elevates the

dehydration of head group (resulting in increased hydrophobic nature

of the molecules) and thermal stability of the BS molecules [37]. Hence,

it is postulated that thermal along with bioeld energy might be acted

at BS molecules which enhanced the hydrophobic nature and thermal

DSC thermogram of control and treated PP are shown in Figure

4. e control PP showed an endothermic peak due to absorbed

water at 122°C and another endothermic inexion was observed at

around 300°C which was due to melting temperature and thermal

denaturation of the control sample. DSC thermogram of treated

sample exhibited a broad endothermic peak at 175°C. Researchers have

found that the major endothermic peak observed (from 0 to 180°C) in

case of soy protein, gelatin, sodium casein and corn gluten meal has

been attributed to loss of residual water or hydrogen bond disruption

between protein molecules [38-41]. Another endothermic peak was

observed at 290°C in the treated PP which was probably due to thermal

denaturation and melting temperature of the protein. e control PP

showed an exothermic temperature peak at around 267°C. However,

the DSC thermogram of treated PP showed (Figure 4) an exothermic

transition at 278°C. Tang et al. during their studies on heat induced

aggregation and denaturation of soy proteins observed much lower

exothermic peaks (150°C) [38]. It is presumed that exothermic peak

might be increased due to bioeld treatment. e native conrmation

of proteins is mainly maintained by its inherent hydrogen bonding and

electrostatic interactions, whereas thermal stability is closely related

to hydrophobic interactions. If the hydrophilic interactions retaining

the tertiary structure of protein are ruptured by heating, hydrophobic

regions initially hidden inside the proteins will be exposed to the protein

surface and subordinate with other hydrophobic protein molecules to

form aggregates. Hence, in this study the exothermic peak could be

attributed to the protein aggregation [42,43]. Additionally, the increase

in exothermic peak may be due bioeld energy which raised the treated

PP aggregation temperature.

XRD diractogram of control and treated BS are illustrated in

Figure 5. e XRD diractogram of control BS showed presence of

broad as well as intense peak. e control sample showed crystalline

peaks at 2θ equals to 31.61°, 45.40°, 56.42°, 66.12° and 75.26°. e

control BS showed a broad peak at 2θ equals to 11.1° which was due

to amorphous nature. ese XRD peaks showed presence of both

crystalline as well as amorphous regions in the control BS (Figure 5).

e treated BS also displayed similar XRD peaks at 2θ equals to 27.2°,

31.63°, 45.39°, 56.45°, 66.14° and 75.10°. e result showed a minimal

reduction in the intensity of the XRD peaks in the treated BS which

may be due to decrease in crystallinity of the sample.

XRD diractogram of control and treated PP are shown in Figure

6. e XRD of control sample showed an amorphous nature which

was conrmed by a broad peak at around 2θ equals to 14.48° and 23°.

However, the treated PP showed (Figure 6) XRD peaks at around 2θ

equals to 28.39° and 32.71° which showed no signicant change in

amorphous nature of the treated PP aer bioeld treatment.

TGA was used to get further insights about the thermal stability of

the control and treated samples (BS and PP). e TGA thermogram

of BS (control and treated) are presented in Figures 7 and 8. e TGA

thermogram of control BS exhibited one step thermal degradation.

e thermal degradation commenced at 80°C and continued up

to 130°C. e sample had lost 3.28% of its total original weight

during this process. is thermal event was probably associated with

Figure 2: Percentage change in particle size of bile salt (BS) and proteose

peptone PP (control and treated).

SampleControl (m2/g)Treated (m2/g)%Change in surface area

Bile salt0.630.62-1.59

Proteose peptone1.001.088.00

Table 1: Surface area analysis of bile salt and proteose peptone.

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Thermal and Physical Properties of Biofield Treated Bile Salt and Proteose Peptone - PdfSR.com

# data[].attributes.attachment_link.description
Bile salt (BS) and proteose peptone (PP) are important biomacromolecules being produced inside the human body. The objective of this study was to investigate the influence of biofield treatment on physicochemical properties of BS and PP. The study was performed in two groups (control and treated).

# data[].attributes.headline
	
	
	
	
	
		 Spectroscopic Test of 1,2,3-Trimethoxybenzene
	

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		 Spectroscopic Test of 1,2,3-Trimethoxybenzene
	

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Volume 4 * Issue 4 * 1000136

ISSN: 2329-6631 JDD an open access journal

Research ArticleOpen Access

Journal of Developing Drugs

Trivedi et al.,J Develop Drugs 2015, 4:4

http://dx.doi.org/10.4172/2329-6631.1000136

Keywords: X-ray diraction; ermal analysis; Fourier transform

infrared (FT-IR) spectroscopy; Ultra violet-visible spectroscopy (UV-

Abbreviations: XRD: X-ray diraction; DSC: Dierential scanning

calorimetry; TGA: ermogravimetric analysis; FT-IR: Fourier

transform infrared; UV-Vis: Ultra Violet-Visible spectroscopy analysis;

CAM: Complementary and alternative medicine

Benzene-based compounds are used as an intermediate for

the synthesis of pesticides and other chemicals. Methoxybenzene

derivatives such as anisole (methoxybenzene) and veratrole

(1,2-dimethoxybenzene) are widely used in perfumes, insect

pheromones, pharmaceuticals [1] and synthesis of organic compounds

[2]. 1,2,3-Trimethoxybenzene is an organic compound with excellent

physical properties due to its interesting chemical structure [3]. It has

been used to study the eect of solvent on photo-induced electron

transfer reactions [4]. 1,2,3-Trimethoxybenzene was used as an

intermediate for the synthesis of 2,4-diamino-5-benzylpyrimidines

and analogues for antimicrobial applications [5]. However,

1,2,3-trimethoxybenzene based compounds are potentially toxic in

nature and causes enormous health problems. ese compounds are

found in many hazardous waste sites that have been proposed for

inclusion in the environmental protection agency (EPA) national

priority list [6]. e distribution and degradation of the chemicals

entirely depend on physicochemical properties of the chemicals. ese

properties are namely molecular weight, solubility, volatilization,

and polarity [7,8]. It was reported that by improving volatilization

of the chemicals it might accelerate the degradation by reacting with

photochemicaly-produced free radicals [9]. Chakraborty et al. used

anaerobic degradation of benzene derivatives by Dechloromonas

strain [10]. However, these methods are not cost eective. us,

some alternate strategies should be designed which can increase the

degradation of 1,2,3-Trimethoxybenzene, ultimately it might reduce

the health problems associated with this compound. Recently, bioeld

energy treatment was used as potential strategy to alter the physical,

chemical and thermal properties of metals [11,12], ceramic [13], organic

compounds [14,15], and organic products [16,17]. erefore, authors

planned to investigate the inuence of bioeld energy treatment on

physical, thermal and spectral properties of 1,2,3-trimethoxybenzene.

e National Centre for Complementary and Alternative Medicine

(NCCAM), which is a part of the National Institute of Health (NIH),

endorses the use of Complementary and Alternative Medicine (CAM)

therapies as an alternative in the healthcare sector, and about 36%

of Americans regularly uses some form of CAM [18]. CAM includes

numerous energy-healing therapies; bioeld therapy is one of the

energy medicine used worldwide to improve overall health.

Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and

pharmaceutical agents. The objective of this study was to investigate the inuence of bioeld energy treatment on the

physical, thermal and spectral properties of 1,2,3-trimethoxybenzene.

Methods: The study was performed by dividing the sample into two groups (control and treated). The control

group remained as untreated, while the treated group received Mr Trivedi's bioeld energy treatment. The control

and treated 1,2,3-trimethoxybenzene samples were then characterized by X-ray diffraction (XRD), differential scanning

calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-

visible spectroscopy (UV-Vis) analysis.

Results: XRD studies revealed the signicant increase in crystallite size of treated sample by 45.96% as compared

to the control sample. DSC analysis showed a decrease in melting temperature of the treated sample (45.93ºC) with

respect to control (46.58ºC). Additionally, the substantial change was evidenced in latent heat of fusion of treated

sample by 64.18% as compared to the control. TGA analysis indicated a decrease in maximum thermal decomposition

temperature (Tmax ) of treated sample (151.92ºC) as compared to the control sample (154.43ºC). This indicated the

decrease in thermal stability of the treated sample as compared to the control. FT-IR spectroscopic analysis showed

an increase in the frequency of C-O bond in treated sample (1105→1174 cm-1 ) as compared to the control sample.

However, UV analysis showed no changes in absorption peaks in treated sample as compared to the untreated sample.

Conclusion: Overall, the result indicated that bioeld energy treatment has altered the physical, thermal and

spectral properties of the treated sample as compared to control. Hence, the treated sample could be used as an

intermediate in the synthesis of organic compounds.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462

Received September 16, 2015; Accepted September 29, 2015; Published

Citation: Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015)

Physical, Thermal and Spectral Properties of Bioeld Treated 1,2,3-Trimethoxybenzene.

J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits un-

restricted use, distribution, and reproduction in any medium, provided the original

author and source are credited.

Physical, Thermal and Spectral Properties of Biofield Treated

Mahendra Kumar Trivedi1, Rama Mohan Tallapragada1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Rakesh Kumar Mishra2 and

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya Pradesh, India

Citation:Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Physical, Thermal and Spectral Properties of Bioeld Treated

1,2,3-Trimethoxybenzene. J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Volume 4 * Issue 4 * 1000135

ISSN: 2329-6631 JDD an open access journal

Researchers have shown that when atoms undergo any

translation, vibration, rotation and quantiable motion a measurable

electromagnetic radiation is generated which is evident by acoustic

or vibrational spectroscopy [19]. Likewise, the cells present in the

human body are consisting of the electron, proton, neutron and

fundamental particles that always remain in a state of vibratory motion

[19]. Additionally, neurons that are present in the human central

nervous system have the ability to transmit the information in the

form of electrical signals [20-23]. us, human bioeld is referred

as an energetic eld or matrix that surrounds the human body. is

energetic eld is identical to superhighway that allows DNA in our

cells to communicate faster than light and maintain coherent, holistic

intelligence in the organism [24]. erefore, it is envisaged that human

beings have the ability to harness the energy from the environment/

Universe and can transmit into any object (living or non-living)

around the Globe. e object(s) will always receive the energy and

responding in a useful manner that is called bioeld energy. Mr Trivedi

is known transform the characteristics of various living and non-living

things using his unique bioeld energy. is bioeld energy treatment

is also known as e Trivedi Eect®. It is known to alter phenotype

characteristics of microbes [25,26] and improved the growth and

anatomical characteristics of medicinal plants [27,28].

Aer considering the potential of bioeld energy treatment and

chemical properties of 1,2,3- trimethoxybenzene, this study was

conducted to investigate the inuence of this treatment on physical,

thermal and spectral properties of the compound.

1,2,3-Trimethoxybenzene was procured from S D Fine Chemicals

Ltd., India. e sample was divided into two parts; one was kept as a

control sample while the other was subjected to Mr Trivedi's unique

bioeld energy treatment and coded as treated sample. e treated

group was in sealed pack and handed over to Mr Trivedi for bioeld

energy treatment under standard laboratory conditions. Mr Trivedi has

given the energy treatment through his energy transmission process

to the treated samples without touching the sample. e control and

treated samples were characterized by X-ray diraction, Dierential

scanning calorimetry, ermogravimetric analysis, Fourier transform

infrared spectroscopy, and Ultra violet-visible analysis.

X-ray diraction (XRD) study: e XRD analysis of control

and treated 1,2,3-trimethoxybenzene were carried out using Phillips,

Holland PW 1710 X-ray diractometer system, which contains a

copper anode with nickel lter. e radiation of wavelength used by the

XRD system was 1.54056 Å. e XRD results obtained were presented

in the form of a chart of 2θ vs. intensity. e average crystallite size (G)

was calculated by using formula:

Here, λ is the wavelength of radiation used; b is full-width half-

maximum (FWHM) of peaks and k is the equipment constant (=0.94).

Percentage change in average crystallite size was calculated using

Percent change in average crystallite size=[(Gt -Gc)/Gc ] × 100

Where, Gc and Gt are the average crystallite size of control and

treated powder samples respectively.

Dierential scanning calorimetry (DSC): e control and treated

1,2,3-trimethoxybenzene samples were analysed using a Pyris-6 Perkin

Elmer DSC at a heating rate of 10ºC/min under air atmosphere, and

the air was ushed at a ow rate of 5 mL/min. Predetermined amount

of sample was kept in an aluminium pan and closed with a lid. A blank

aluminium pan was used as a reference. e percentage change in

latent heat of fusion was calculated using following equations:

% change in Latent heat of fusion 100

Where, ΔH Control and ΔH Treated are the latent heat of fusion of

control and treated samples, respectively.

ermogravimetric analysis-Dierential thermal analysis

(TGA-DTA): e thermal stability of control and treated

1,2,3-trimethoxybenzene were analysed by using Mettler Toledo

simultaneous TGA and Dierential thermal analyser (DTA). e

samples were heated from room temperature to 400ºC using a heating

rate of 5ºC/min under air atmosphere.

FT-IR spectroscopy: e FT-IR spectra were recorded on

Shimadzu's Fourier transform infrared spectrometer (Japan) with the

frequency range of 4000-500 cm-1 . e analysis was accomplished to

evaluate the eect of bioeld treatment at an atomic level like dipole

moment, force constant and bond strength in chemical structure [29].

e treated sample was divided into two parts T1 and T2 for FT-IR

UV-Vis spectroscopic analysis: UV spectra of the control and

treated 1,2,3-trimethoxybenzene samples were acquired on Shimadzu

UV-2400 PC series spectrophotometer with 1 cm quartz cell and a

slit width of 2.0 nm. Methanol was used as a solvent and the analysis

was carried out using wavelength in the range of 200-400 nm. e UV

spectra were analysed to determine the eect of bioeld treatment

on the energy gap of highest occupied molecular orbital and lowest

unoccupied molecular orbital (HOMO-LUMO gap) [29]. e treated

sample was divided in two parts T1 and T2 for the analysis.

e XRD was used to investigate the crystalline nature of control

and treated 1,2,3-trimethoxybenzene sample. e XRD diractogram

of control and treated 1,2,3-trimethoxybenzene are presented in Figure

1. XRD diractogram of control 1,2,3-trimethoxybenzene showed

intense crystalline peaks at 2θ equal to 13.05º, 13.29º, 13.54º, 19.54º,

21.67º, 22.75º, 25.55º, 25.77º, 26.78º and 27.20º. Whereas, the treated

1,2,3-trimethoxybenzene also showed XRD peaks at 2θ equal to 13.50º,

13.68º, 19.24º, 22.99º, 26.03º, 27.09º and 27.31º. e comparative

evaluation of the XRD diractograms showed the decrease in

intensity of XRD peaks of treated samples with respect to the control.

It is assumed that bioeld energy treatment might disturb the long-

range pattern of the 1,2,3-trimethoxybenzene molecules that led to a

reduction in crystallinity of the sample.

e crystallite size of control and treated 1,2,3-trimethoxybenzene

was computed using Scherrer formula, and data are depicted in

Figure 2. e crystallite size is known as a group of molecules or

atoms having orientation in the same plane. It was reported that

crystallite size directly inuences the materials properties, and it is

one of the crystallographic parameters linked with the formation of

dislocations and point defects in a crystal structure [30]. e crystallite

size of control 1,2,3- trimethoxybenzene was 67.23 nm, and it was

substantially increased up to 98.13 nm in the treated sample. e result

indicated 45.96% increase in the crystallite size of treated compound

as compared to the control sample. Pang and Bao reported that due

Citation:Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Physical, Thermal and Spectral Properties of Bioeld Treated

1,2,3-Trimethoxybenzene. J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Volume 4 * Issue 4 * 1000135

ISSN: 2329-6631 JDD an open access journal

to synthesis temperature and ripening on hydroxyapatite causes an

increase in crystallite size [31]. Rey et al. [32] and Drouet et al. [33]

reported an increase in crystal size with increase in temperature and

pH. Hence, it is presumed here that bioeld treatment might provide

thermal energy to the treated 1,2,3-trimethoxybenzene atoms or

molecules that lead to a reduction in dislocation density and increase

in crystallite size as compared to the control. Carballo et al. reported

that the rate of reaction can be signicantly improved by an increase

in crystallite size [34]. us, it is assumed that the increased crystallite

size of treated 1,2,3-trimethoxybenzene might accelerate its reaction

rate and percentage reaction yield during the synthesis of compounds.

Additionally, the increased crystallite size can be correlated with

increase in particle size and hence decrease solubility [35]. Hence, it

is assumed that this might increase the volatilization leading to faster

degradation of the treated sample as compared to control.

DSC study: DSC was used to investigate the latent heat of fusion and

melting nature of the control and treated samples. DSC thermogram

of control and treated 1,2,3-trimethoxybenzene are presented in

Figure 3. e DSC thermogram of control 1,2,3-trimethoxybenzene

showed a sharp endothermic inexion at 46.58ºC that was due

to melting temperature of the sample. However, the treated

1,2,3-trimethoxybenzene showed an endothermic peak at 45.93ºC.

is indicated the decrease in melting temperature of the treated

sample with respect to the control. It was reported that if the molecules

contributing to a lattice are rigid, compact and symmetrical they tend

to maximize the intermolecular interactions. us, a compound with

less compact molecular arrangement has a low melting temperature

than compactly arranged compound i.e., more energy is needed to

separate the molecules [36]. Hence, it is assumed that bioeld treated

sample might have a less compact arrangement that lead to the decrease

e latent heat of fusion was computed from the DSC thermogram

and data are presented in Table 1. e control compound showed a latent

heat of fusion of 104.28 J/g and it was increased signicantly to 171.21

J/g in the treated sample. e results indicated a signicant increase in

latent heat of fusion by 64.18% as compared to the control sample. e

latent heat of fusion is considered as the energy required to overcome

Figure 1: XRD diffractograms of control and treated 1,2,3-trimethoxybenzene.

Figure 3: DSC thermograms of control and treated 1,2,3-trimethoxybenzene.

Figure 2: Crystallite size of control and treated 1,2,3-trimethoxybenzene.

ParameterControl Treated

Latent heat of fusion ΔH (J/g)104.28171.21

Melting temperature (ºC)46.5845.93

Weight loss (%)53.9357.04

Table 1: Thermal analysis data of control and treated 1,2,3-trimethoxybenzene.

Tmax : Maximum thermal decomposition temperature.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene - PdfSR.com

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 1,2,3-trimethoxybenzene. Methods: The study was performed by dividing the sample into two groups (control and treated).

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	 1,2,3 Trimethoxybenzene- Impact of Spiritual Energy
	

# data[].attributes.subject
	
	
	
	
	 1,2,3 Trimethoxybenzene- Impact of Spiritual Energy
	

# data[].attributes.body
| Want to know how the physical, thermal and spectral properties of 1,2,3-Trimethoxybenzene can be altered? Visit here to get information about the science used to alter the properties and results.

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fourier transform infrared (ft-ir) spectroscopy

# data[].attributes.attachment_link.keywords[]
spectral properties of 1 2 3-trimethoxybenzene

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 1,2,3-trimethoxybenzene. Methods: The study was performed by dividing the sample into two groups (control and treated).

# data[].attributes.headline
	
	
	
	
	
		 DSC Analysis of 1,2,3 Trimethoxybenzene
	

# data[].attributes.subject
	
	
	
	
	
		 DSC Analysis of 1,2,3 Trimethoxybenzene
	

# data[].attributes.body
| Study shows alteration in DSC analysis of 1,2,3 Trimethoxybenzene by significant decrease in melting temperature compared to treated sample.

# data[].attributes.headline
	
	
	
	
	
		 Alteration of Spectral Properties of 1,2,3-Trimethoxybenzene
	

# data[].attributes.subject
	
	
	
	
	
		 Alteration of Spectral Properties of 1,2,3-Trimethoxybenzene
	

# data[].attributes.body
| Study speculate that human energy might altered the spectral properties of 1,2,3-trimethoxybenzene by signiﬁcantly increases the latent heat.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and

# data[].attributes.headline
	
	
	
	
	 Evaluation of 1,2,3-Trimethoxybenzene Properties
	

# data[].attributes.subject
	
	
	
	
	 Evaluation of 1,2,3-Trimethoxybenzene Properties
	

# data[].attributes.body
| Study shows the alteration of 1,2,3 Trimethoxybenzene properties in physical, thermal and spectral aspects. For more details visit the link.

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		Human Energy Treatment of 1,2,3 Trimethoxybenzene
	

# data[].attributes.subject
	
	
	
	
	
		Human Energy Treatment of 1,2,3 Trimethoxybenzene
	

# data[].attributes.body
| The main objective of this study is to investigate the influence of unique human energy on the physical & thermal properties of 1,2,3-trimethoxybenzene.

# data[].attributes.headline
	
	
	
	
	
		 Streptococcus Agalactiae MIC Alteration using Unique Human Energy
	

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		 Streptococcus Agalactiae MIC Alteration using Unique Human Energy
	

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| The outcomes of this study shows alterations in MIC values of tested antimicrobials against S. agalactiae gr. B after the unique human energy treatment.

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	Study of Streptococcus Agalactiae Characteristics Alterations
	

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	Study of Streptococcus Agalactiae Characteristics Alterations
	

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|  Read here how human energy treatment has altered the Streptococcus Agalactiae Characteristics. Read here for more details.

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Volume 7(5): 269-273 (2015) - 269

ISSN: 1948-5948 JMBT, an open access journal

Research ArticleOpen Access

Trivedi et al., J Microb Biochem Technol 2015, 7:5

http://dx.doi.org/10.4172/1948-5948.1000223

Research ArticleOpen Access

Microbial & Biochemical Technology

*Corresponding author:Snehasis Jana, Trivedi Global Inc., 10624 S Eastern

Avenue Suite A-969, Henderson, NV 89052, USA, Tel: +91-755-6660006; E-mail:

Received August 12, 2015; Accepted September 01, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015)

Effect of Bioeld Energy Treatment on Streptococcus group B: A Postpartum

Pathogen. J Microb Biochem Technol 7: 269-273. doi: 10.4172/1948-5948.1000223

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial

septicemia and neonatal meningitis. The current study was attempted to investigate the effect of bioeld treatment

on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae

gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number

and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains

of S. agalactiae gr. B were selected for the study. Gr. I was considered as control. Both revived (Group; Gr. II)

and lyophilized (Gr. III) strains of S. agalactiae gr. B were subjected to Mr. Trivedi's bioeld treatment. Gr. II was

assessed on day 5 and day 10 while Gr. III on day 10 with respect to the control (Gr. I) using MicroScan Walk-Away®

system. Although bioeld treatment did not show any change with respect to susceptibility pattern. However the

minimum inhibitory concentration of S. agalactiae gr. B showed signicant (70.37%) alteration, out of twenty-seven

in lyophilized group (Gr. III) as compared to the control. Moreover, the improvement of MIC value of noroxacin was

observed by two-fold (8 to ≤4 µg/mL) in Gr. II on day 10 after bioeld energy treatment as compared to the control.

It was observed that overall 48.28% biochemical reactions, out of twenty-nine were altered in Gr. II with respect to

the control. Moreover, biotype numbers were changed in Gr. II on day 5 (777777615) and on day 10 (757677405)

as compared to the control (237147047). The results suggest that bioeld treatment has signicant impact on S.

agalactiae gr. B in revived treated cells (Gr. II) with respect to MIC values, biochemical reactions pattern and biotype

Effect of Biofield Energy Treatment on

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Harish Shettigar1, Sambhu Charan Mondal2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Madhya Pradesh, India

Minimum inhibitory concentration; Biochemical reaction; Biotype

Abbreviations: CLSI: Clinical and laboratory standards institute;

GBS: Group B Streptococci; CAMP: Christie-Atkins-Munch-Petersen;

CDC: Centers for Disease Control and Prevention; ACOG: American

College of Obstetricians and Gynecologists; AAP: American Academy

of Pediatrics; MIC: Minimum inhibitory concentration; ATCC:

American Type Culture Collection; PBPC 20: Positive Breakpoint

Group B Streptococci (GBS), or Streptococcus agalactiae group B,

are Gram-positive, non-spore forming, non-motile, β-hemolytic and

chain-forming cocci bacteria. It is mainly inhabitant in human gut ora

and female urogenital tract [1]. Pregnant women [2] and neonates [3] are

the main victim host of this organism. It can be transferred to neonates

through the birth canal and causes bacterial septicaemia and neonatal

meningitis [4]. Most of GBS, produce Christie-Atkins-Munch-Petersen

(CAMP) factor or protein B an extracellular cytolytic protein and

β-lysin from Staphyllococcus species are jointly lysed the erythrocytes

[5]. It is estimated that in US over 70,000 cases of GBS diseases

were prevented [6]. Several antibiotics such as penicillin, ampicillin,

amoxicillin, cephalosporins (all three generations), macrolides,

clindamycin and vancomycin (as alternative) have been used to treat

against GBS infections. Among above mentioned antibiotics penicillin

is the drug of choice next to cefazolin. However, it also have certain

limitations such as high minimum inhibitory concentration (MIC), a

factor associated with high level of bacteremia and more concentration

of microbes in tissue, especially in cerebrospinal uid [7,8]. erefore,

some alternative treatment strategies are needed to overcome these

lacunas against β-hemolytic strain of gr. B Streptococci. Bioeld

treatment has been known as an alternative approach that may be

useful for S. agalactiae group B infected patients.

Researchers have shown that short-lived electrical events or action

potential exist in the several type of mammalian cells such as neurons,

muscles, and endocrine cells [9]. For instance, when the cells present

in central nervous system of human body communicate with each

another by means of electrical signals that propagate along the nerve

impulses. erefore, it was hypothesized that bioeld exists around

the human body and evidence was found using electromyography,

electrocardiography and electroencephalogram [10]. us, the human

body emits the electromagnetic waves in the form of bio-photons,

which surrounds the body and it is commonly known as bioeld.

erefore, the bioeld consists of electromagnetic eld, being generated

by moving electrically charged particles (ions, cell, molecule etc.) inside

the human body. Rivera-Ruiz reported that electrocardiography has

been extensively used to measure the bioeld of human body [11].

us, human has the ability to harness the energy from environment or

Universe and can transmit into any living or nonliving object(s) around

the Globe. e objects always receive the energy and responding into

useful way that is called bioeld energy and the process is known as

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Effect of Bioeld Energy Treatment on Streptococcus group B: A

Postpartum Pathogen. J Microb Biochem Technol 7: 269-273. doi:10.4172/1948-5948.1000223

Volume 7(5): 269-273 (2015) - 270

ISSN: 1948-5948 JMBT, an open access journal

bioeld treatment that is also called as Trivedi eect®. Mr. Trivedi's

unique bioeld treatment has been known to transform the structural,

physical, and thermal properties of several metals in material science

[12-14], improved the overall productivity of crops [15,16], altered

characteristics features of microbes [17-19] and improved growth and

anatomical characteristics of various medicinal plants [20,21].

Due to the clinical signicance of this organism and literature

reports on bioeld treatment, the present work was undertaken to

evaluate the impact of bioeld treatment on S. agalactiae group B

in relation to antimicrobials susceptibility and bio typing based on

various biochemical characters.

S. agalactiae group B, American Type Culture Collection (ATCC

12386) strains were procured from MicroBioLogics, Inc., USA, in

two sets A and B. Two dierent sealed packs were stored with proper

storage conditions until further use. All the tested antimicrobials and

biochemicals were procured from Sigma-Aldrich, MA, USA. e

antimicrobial susceptibility, biochemical reactions and biotype number

were estimated with the help of MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA, USA) using Positive Breakpoint Combo 20

(PBPC 20) panel with respect to the control group.

Two ATCC samples A and B of S. agalactiae gr. Bwere grouped

(Gr.). ATCC A sample was revived and divided into two parts Gr.I

(control) and Gr.II (revived); likewise, ATCC B was labeled as Gr.III

Bioeld treatment strategy

e Gr. I remained as untreated. e treatment Gr. II and III

in sealed pack were handed over to Mr. Trivedi for bioeld energy

treatment under laboratory condition. Mr. Trivedi provided the

treatment through his energy transmission process which includes

bioenergy emission to the treated groups (Gr. II and Gr. III) without

touching the samples. Aer treatment, sample was handed over in the

same condition and stored at standard conditions as per the standard

experimental protocol. An optimum precautionary measure were taken

while evaluating the study parameters throughout the experiments.

e dierences in parameters before and aer the treatment were

noted and compared. Gr.II was assessed at two time point i.e. on day

5 and day 10, while Gr. III was assessed on day 10 for antimicrobial

susceptibility, MIC, biochemical reactions pattern, and biotyping.

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of S. agalactiae gr. B

was carried out with the help of automated instrument, MicroScan

Walk-Away® system using PBPC 20 panel as per the clinical and

laboratory standards institute (CLSI) guidelines. e test was carried

out on MicroScan, which was miniaturized of the broth dilution

susceptibility test that has been dehydrated. Briey, the standardized

suspension of S. agalactiae gr. Bwas inoculated, rehydrated, and then

subjected to incubation for 16 hours at 35°C. e detailed experimental

procedures and conditions were followed as per the manufacturer's

instructions. e antimicrobial susceptibility pattern and MIC were

determined by observing the lowest antimicrobial concentration

showing growth inhibition [22].

Biochemical reaction studies

Biochemical reactions of S. agalactiae gr. Bwere determined using

MicroScan Walk-Away®, system with PBPC 20 panel [22]. Biochemicals

used in the study were arabinose, arginine, bacillosamine, bile

esculin, β-lactamases, crystal violet, hemolysin, indoxyl phosphatase,

inulin, acidication lactose, mannitol, mannose, micrococcus screen,

sodium chloride, nitrate, novobiocin, optochin, p-nitro phenyl β-D-

glucuronide, p -nitro phenyl β-D-galactopyranoside, phosphatase,

pyruvate, pyrolidonyl arylamidase, ranose, rambose, sorbitol,

thymidine free growth, acidication trehalose, urea, and Voges-

Identication of organism by biotype number

e biotype number of S. agalactiae gr. Bwas determined on

MicroScan Walk-Away® processed panel data report with the help of

biochemical reactions data [22].

Antimicrobial susceptibility test

e outcomes of MIC values of tested antimicrobials against S.

agalactiae gr. B aer bioeld treatment are summarized in Table 1. e

data were analyzed using automated system and compared with respect

to the control. In this experiment, twelve antibiotics were used to

investigate the susceptibility pattern on GBS viz. ampicillin, cefepime,

cefotaxime, ceriaxone, chloramphenicol, clindamycin, levooxacin,

linezolid, ooxacin, penicillin, tetracycline, and vancomycin. Out

of these, ve antibiotics showed susceptible in the control sample. It

has been evidenced from literatures that penicillin and its derivatives

are the choice of drugs against gr. B streptococcal infection [8,9].

However, bioeld treatment did not show any alteration with respect

to susceptibility pattern in all the treated groups aer bioeld treatment

(data not shown). Besides sensitivity assay, the MIC values of tested

antimicrobials were signicantly (70.37%) altered out of twenty seven

as compared to the control. e MIC value of noroxacin was reduced

by two-fold to ≤ 4 µg/mL aer bioeld treatment in Gr. II on day 10

as compared to the control (8 µg/mL). e MIC values of cefazolin,

cephalothin, and chloramphenicol were changed from ≤ 8 to >16 µg/

mL in Gr. II on day 5 and 10 as compared to the control. e MIC values

of rifampin and synercid were changed from ≤ 1 to >2 µg/mL in Gr. II

on day 5 and 10 as compared to the control. Alteration of MIC values of

cefotaxime and ceriaxone were changed from ≤8 to 32 µg/mL in Gr.

II on day 5 and 10 as compared to the control. Moreover, MIC values

of amoxicillin/k-clavulanate and trimethoprim/sulfamethoxazole

were slightly changed from ≤4/2 to >4/2 µg/mL (on day 5 and 10) and

≤2/38 to >2/38 µg/mL (on day 5) respectively in Gr. II aer bioeld

treatment as compared to the control. Antimicrobial linezolid showed

an alteration of MIC value in Gr. II on day 5 (>4 µg/mL) and on day

10 (4 µg/mL) as compared to the control. Besides this, alteration of

MIC values were observed in case of penicillin (≤ 0.03 to >8 µg/mL),

vancomycin (≤ 0.2 to >16 µg/mL), nitrofurantoin (≤ 32 to >64 µg/mL),

clindamycin (≤ 0.5 to >2 µg/mL), ampicillin (≤ 0.25 to >8 µg/mL) and

ampicillin/sulbactam (≤ 8/4 to >16/8 µg/mL) in Gr. II on day 5 and 10

as compared to the control. e MIC value of tetracycline was changed

from ≤ 4 to 8 µg/mL in Gr. II on day 10 and MIC value of oxacillin

was changed from ≤ 0.25 to >2 µg/mL (on day 5) and 2 µg/mL on day

10 in Gr. II as compared to control. Antimicrobials did not show any

change in MIC value in Gr. III as compared to the control aer bioeld

treatment. Seventeen out of twenty seven (62.96%) antimicrobials

showed alteration of MIC value in Gr. II on day 5 and 66.67% (eighteen

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Effect of Bioeld Energy Treatment on Streptococcus group B: A

Postpartum Pathogen. J Microb Biochem Technol 7: 269-273. doi:10.4172/1948-5948.1000223

Volume 7(5): 269-273 (2015) - 271

ISSN: 1948-5948 JMBT, an open access journal

such as ranose (RAF) and sorbitol (SOR) showed an alteration of

biochemical reaction i.e. negative (-) to positive (+) in Gr. II on day 5

while remained negative in Gr. II on day 10 and in Gr. III as compared

e one of the key characteristic feature for GBS is complete

lysis of red blood cells and metabolization of sugars by fermentation

process. Hence, in control sample the positive reaction of hemolysin

(HEM) indicated β-hemolysis of erythrocytes and positive reaction of

acidifying lactose (LAC) indicated the production of lactic acid as bi-

product. e data was supported with literature [23]. Similarly, based

on existing literature pyrolidonyl arylamidase (PYR) has negative

reaction in group B streptococcal species while positive reaction in

group A streptococcal. Control data of PYR was well supported with

literature data [24]. However, aer bioeld treatment the negative

reaction was altered in Gr. II, assessed on day 5 as well as day 10 due to

change of enzymatic reaction.

Overall, 48.28% biochemical reactions were altered in tested

twenty-nine biochemicals with respect to the control aer bioeld

treatment. In both time points of Gr. II (day 5 and 10) 41.38% (twelve

out of twenty-nine) biochemical reactions were altered as compared

to the control. About 51.72% out of twenty-nine biochemicals, such as

arabinose (ARA), bacillosamine (BAC), β-lactamase (BL), hemolysin

(HEM), indoxyl phosphatase (IDX), inulin (INU), acidication lactose

(LAC), mannose (MNS), micrococcus screen (MS), optochin (OPT),

glycosidase (PGT), phosphatase (PHO), thymidine free growth (TFG),

acidication trehalose (TRE), and Voges-Proskauer (VP) did not

out of twenty seven) on day 10 as compared to the control aer bioeld

treatment. Eight, out of twenty seven tested antimicrobials (29.63%)

viz. cefepime, ciprooxacin, gatioxacin, imipenem, levooxacin,

moxioxacin, ooxacin and piperacillin/tazobactam did not show any

alteration of MIC values in all the treated cells of GBS as compared to

Biochemical reactions studies

Study of biochemical reactions can be utilized to identify

the enzymatic and metabolic characteristic features of microbes.

Microorganisms can be categorically dierentiated based on their

utilization of specic biochemicals as nutrients during the process of

metabolism or enzymatic reactions. e specic biochemical showed

some changes against S. agalactiae gr. Baer bioeld treatment that

are shown in Table 2. Biochemicals such as bile esculin (BE), crystal

violet (CV), mannitol (MAN), sodium chloride (NaCl), nitrate (NIT),

novobiocin (NOV), pyrolidonyl arylamidase (PYR) and urea (URE)

were changed from negative (-) to positive (+) reactions in revived

treated group (Gr. II) on day 5 and 10, but remained same i.e. negative

(-) reaction in lyophilized treated cells (Gr. III) with respective to

control. Arginine (ARG) and glycosidase (PGR) were converted

from positive (+) to negative (-) reactions in Gr. II on day 10, while

remained unchanged i.e. positive (+) on day 5 in Gr. II and in Gr. III as

compared to the control in bioeld treated S. agalactiae gr. B. Similarly,

pyruvate (PRV), and rambose (RBS) were converted from positive (+)

to negative (-) reactions on both days in Gr. II, while did not show any

change in Gr. III as compared with the control. Moreover, biochemicals

S. No.AntimicrobialType of Response

10. Ciprooxacin≤1 ≤1 ≤1 ≤1

12. Gatioxacin≤2 ≤2 ≤2 ≤2

14. Levooxacin≤2 ≤2 ≤2 ≤2

16. Moxioxacin≤2 ≤2 ≤2 ≤2

22. Piperacillin/tazobactam≤4 ≤4 ≤4 ≤4

MIC values are presented in µg/mL; Gr.: Group

# data[].attributes.attachment_link.title
Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen - PdfSR.com

# data[].attributes.attachment_link.description
Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

# data[].attributes.headline
	
	
	
	
	
		 Alteration of Gr.II Streptococcus Agalactiae Biotype Number
	

# data[].attributes.subject
	
	
	
	
	
		 Alteration of Gr.II Streptococcus Agalactiae Biotype Number
	

# data[].attributes.attachment_link.title
"Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

# data[].attributes.headline
	
	
	
	
	 Impact of Biochemical Reaction on gr. B Streptococcus Agalactiae 
	

# data[].attributes.subject
	
	
	
	
	 Impact of Biochemical Reaction on gr. B Streptococcus Agalactiae 
	

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| Read the impact of biochemical & biotyping reaction of group B Streptococcus Agalactiae.

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	 Streptococcus Agalactiae Alterations after Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	 Streptococcus Agalactiae Alterations after Biofield Treatment
	

# data[].attributes.attachment_link.title
Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen

# data[].attributes.attachment_link.description
Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with

# data[].attributes.headline
	
	
	
	
	Impact of Biofield Treatment on Group B Streptococcus Agalactiae
	

# data[].attributes.subject
	
	
	
	
	Impact of Biofield Treatment on Group B Streptococcus Agalactiae
	

# data[].attributes.body
|  Read here how human energy treatment has altered the Streptococcus Agalactiae Characteristics. Read here for more details.

# data[].attributes.headline
	
	
	
	
	
		 Analysis of Streptococcus Agalactiae Biochemical Characteristics
	

# data[].attributes.subject
	
	
	
	
	
		 Analysis of Streptococcus Agalactiae Biochemical Characteristics
	

# data[].attributes.body
| Study suggest that biofield treatment has significant impact on biochemical characteristics of Streptococcus Agalactiae  For more details visit the link.

# data[].attributes.headline
	
	
	
	
	Study of 16s rDNA Sequencing of Nocardia Otitidis 
	

# data[].attributes.subject
	
	
	
	
	Study of 16s rDNA Sequencing of Nocardia Otitidis 
	

# data[].attributes.body
| The 16S rDNA sequencing of human energy treated Nocardia otitidis sample was carried out to correlate the phylogenetic relationship of N. otitidis with other bacterial species. Follow the link for more details.

# data[].attributes.attachment_link.title
Biological Systems: Open Access Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	 Antibiogram, Phylogenetic & Genotype Analysis Nocardia Otitidis
	

# data[].attributes.subject
	
	
	
	
	 Antibiogram, Phylogenetic & Genotype Analysis Nocardia Otitidis
	

# data[].attributes.body
| Visit the link to know the influence of biofield treatment on antibiogram, phylogenetic & genotype characteristics of Nocardia Otitidis.

# data[].attributes.attachment_link.title
Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Academic Room

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	 DNA Polymorphism of Nocardia otitidis & Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	 DNA Polymorphism of Nocardia otitidis & Biofield Treatment
	

# data[].attributes.body
| The results of this study suggested the impact of biofield treatment on MIC, 16S rDNA analysis, and DNA polymorphism of N. Otitidis. Read here the more details

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Biological Systems: Open Access

Trivedi et al,, Biol Syst Open Access 2015, 4:2

http://dx.doi.org/10.4172/2329-6577.1000143

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract.

Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to

pattern, minimum inhibitory concentration (MIC), DNA polymorphism by Random Amplied Polymorphic DNA (RAPD)

and 16S rDNA sequencing. The strain of N. otitidis (ATCC 14630) was divided into two parts, control and treated.

Antimicrobial susceptibility was studied using the broth microdilution technique. Overall, the MIC values of 16.67%

antimicrobials were changed in the treated group of N. otitidis as compared to the control. Moreover, MIC value of

trimethoprim/sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 µg/mL) in the bioeld energy treated

sample as compared to the control without alteration in the sensitivity spectrum. The 16S rDNA analysis showed that

the treated sample was detected as Enterobacter aerogenes strain NCTC10006T (GenBank Accession No: AJ251468)

with 98% identity of gene sequencing data. However, the nearest homolog genus-species was found as Kluyvera

34 to 53% of polymorphism among treated samples as compared to the control. The 16S rDNA sequencing of treated

sample was carried out to correlate the phylogenetic relationship of N. otitidis with other bacterial species. These

results suggested that Mr. Trivedi's bioeld energy treatment has a signicant impact on N. otitidis.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006, E-mail:

Received August 31, 2015; Accepted September 21, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, et al. (2015)

Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Bioeld Treated

Nocardia otitidis. Biol Syst Open Access 4: 143. doi:10.4172/2329-6577.1000143

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

nocardiosis [9] in immunocompromised patients. Infectious Diseases

Society of America (IDSA) reported that between 500 and 1,000 cases

of Nocardia l infections are recognized in the United States each year, of

which 85% are serious pulmonary or systemic infections [10].

Since 1940s, the sulfonamides have been the drugs of choice for

the treatment of nocardiosis [11]. Due to high mortality rate (50%)

in patients with central nervous system (CNS) Nocardia infections

(Nocardia brain abscess) and patients with non-CNS overwhelming or

disseminated disease, the treatment strategy is inadequate. However, the

combination of sulfamethoxazole with trimethoprim is oen used as the

drug of choice for the treatment of nocardiosis [12]. erefore, some

alternative strategies are needed to treat against nocardiosis. Bioeld

energy has been known as an alternative approach which may be useful

as an alternative treatment to Nocardia infected patients. National

Institute of Health/National Center for Complementary and Alternative

Medicine (NIH/NCCAM) have reported that bioeld (putative energy

elds) or electromagnetic based energy therapies were commonly

used to promote the health and healing [13]. Harold Saxton Burr had

performed the detailed studies on the correlation of electric current

with the physiological process and concluded that every single process

in the human body had an electrical signicance [14]. Recently, it was

discovered that all the electrical processes happening in the human

body have strong relationship with the magnetic eld as required by

susceptibility; Bioeld energy treatment; 16S rDNA sequencing;

Random amplied polymorphic DNA

Abbreviations: NIH/NCCAM: National Institute of Health/

National Center for Complementary and Alternative Medicine;

ATCC: American Type Culture Collection; MIC: Minimum Inhibitory

Concentration; OTUs: Operational Taxonomic Units; NCBI: National

Center for Biotechnology Information; MEGA: Molecular Evolutionary

Genetics Analysis; PCR: Polymerase Chain Reaction; RDP: Ribosomal

Database Project; RAPD: Random Amplied Polymorphic DNA; CNS:

e genusNocardiais associated with the group of microorganisms

known as the aerobic actinomycetes and belongs to the family of

dier from the mycobacteria by the possession of short-chain (40 to

60 carbons) mycolic acids [1]. Nocardia otitidis (N. otitidis) is a weak

lamentous Gram-positive, catalase-positive, branching rods shaped

bacterium that appears similar to Actinomyces species. However, it

can usually be dierentiated from Actinomyces by acid-fast staining

[2]. e taxonomic history of the genus Nocardia is controversial [3].

Nocardia typically exhibits varying degrees of acid fastness due to the

presence of cell wall mycolic acid. e genus is typically similar to the

genus of Mycobacterium. Mordarska et al. had studied the short-chain

fatty acids content in the cell wall of Nocardia and Mycobacterium

genera based on gas-liquid chromatography analysis did not nd

any dierence between two genera [4]. Based on immunoblot and

enzyme-linked immunosorbent assay (ELISA) techniques that detect

specic antibodies that appear as common in various Nocardia and

Actinomadura species. ese antigens do not react with the antibodies

produced in response to Mycobacterium tuberculosis infections [5].

Most of Nocardial infections occur in the United States due to inhalation

of airborne spores or mycelial fragments from the environmental

sources [6]. e most common manifestation of Nocardial disease is

pulmonary nocardiosis [7], extrapulmonary disease [8], and ocular

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Mondal SC, et al. (2015) Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

Ampere's law, which states that the moving charge produces magnetic

elds in the surrounding space [15,16]. us, a human body emits the

electromagnetic waves in the form of bio-photons that is also known

as ultra-weak photon emissions (UPE). It surrounds the body and it

is commonly known as bioeld. erefore, the bioeld consists of an

electromagnetic eld, being generated by moving electrically charged

particles (ions, cell, molecule, etc.) inside the human body [17]. e

transfer of information from cell to cell or DNA or storage by biophotons

has been demonstrated in plants, bacteria, animal neutriophil

granulocytes and kidney cells [18]. Prakash et al. in 2015 reported that the

various scientic instruments such as Kirlian photography, polycontrast

interference photography (PIP) and resonance eld imaging (RFI) can

be extensively used to measure the bioeld of human body [19]. us,

human has the ability to harness the energy from the environment or

universe and can transmit into any living or nonliving object(s) around

the Globe. e objects always receive the energy and responding into

the useful way that is called bioeld energy and the process is known as

bioeld energy treatment. Mr. Mahendra Trivedi's unique bioeld energy

treatment (e Trivedi Eect®) has been known to improve the overall

productivity of crops [20,21], altered characteristics features of microbes

[22-24], alter the structural, physical and thermal properties of several

metals [25,26], and improved growth and anatomical characteristics of

various medicinal plants [27,28].

Based on clinical signicance of N. otitidis and signicant impact

of Mr. Trivedi's bioeld energy modality on microbes, the present work

was undertaken to evaluate the impact of Mr. Trivedi's bioeld energy

on N. otitidis in relation to antimicrobials susceptibility, minimum

inhibitory concentration (MIC), random amplied polymorphic DNA

analysis (RAPD) and 16S rDNA sequencing.

N. otitidis, American Type Culture Collection (ATCC 14630) strain

was procured from Bangalore Genei, Bangalore-India in two vials A

and B. Two dierent sealed packs were stored with proper storage

conditions until further use. All the tested antimicrobials were procured

from Sigma-Aldrich, India. e antimicrobial susceptibility and

MIC were estimated with the help of broth micro dilution technique

as per the Clinical Laboratory Standards Institute (CLSI) guidelines

document number M24-A [29]. e 16S rDNA sequencing and DNA

ngerprinting (RAPD) studies were carried out using Ultrapure

Genomic DNA Prep Kit; Cat KT 83 (Bangalore Genei, India).

Experimental design and bioeld treatment strategy

N. otitidis strain was divided into two groups, i.e., control and

treated. e treated group was in sealed pack and handed over to Mr.

Trivedi for bioeld energy treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated group without touching the sample. Aer treatment,

control and treated groups were assessed on day 10 for antimicrobial

susceptibility, and MIC. For RAPD analysis three inoculums (one for

control and the other two for treated named as treated A and B) were

prepared from N. otitidis samples. ese two bioeld treated samples

A and B were sub-cultured by taking 1% inoculum and inoculated to

fresh 5 ml medium and labeled as treatment A-1 and treatment B-1,

respectively. e result of treated sample was compared with respect to

the control. e 16S rDNA analysis was performed on bioeld treated

samples A and its subcultured sample A1.

Antimicrobial susceptibility test

e antimicrobial susceptibility testing is important for clinically

signicant species. e investigation of antimicrobial susceptibility of N.

otitidis was carried out with the help of broth micro dilution technique,

as per CLSI guidelines. Broth micro dilution was recommended

for isolates of rapidly growing mycobacteria (RGM) based on CLSI

published guidelines and recommendations for testing of non-

tuberculous mycobacteria (CLSI, M24-A, 2003) [29]. e detailed

experimental procedure and conditions were followed as per the

manufacturer's instructions. e antimicrobial susceptibility pattern

(S: Susceptible, R: Resistant) and minimum inhibitory concentration

(MIC) were determined by observing the lowest antimicrobial

concentration showing inhibition of growth.

Random amplied polymorphic DNA (RAPD) analysis

For DNA ngerprinting (RAPD) analysis all the treated samples

(A, A1, B, and B1) were incubated at 37°C with 160 rpm for 18 h.

Subsequently, the cultures were spun down, pelleted at 5000 rpm at

40°C for 10 minutes and the genomic DNA was isolated for control

and treated samples using Genomic DNA Prep Kit (Bangalore Genei,

India). RAPD was performed with all samples of N. otitidis using ve

RAPD primers, which were labelled as RBA8A, RBA13A, RBA20A,

RBA10A and RBA15A were adopted from earlier studies. e PCR

mixture contained 2.5 μL each of buer, 4.0 mM each of dNTP, 2.5 μM

each of primer, 5.0 μL (approximately 20 ng) of each genomic DNA,

2U each of Taq polymerase, 1.5 μL of MgCl2 and 9.5 μL of water in

a total of 25 μL with the following PCR amplication protocol; initial

denaturation at 94°C for 7 min, followed by 8 cycles of denaturation at

94°C for 1 min, annealing at 35°C for 1 min, and extension at 72°C for 2

min; and 35 cycle of denaturation at 94°C for 1 min, annealing at 38°C

for 1 min, and extension at 72°C for 1.5 min; and the nal extension

at 72°C for 7 min. Amplied PCR products from all samples (control

and treated) were separated on 1.5% agarose gels at 75 volts, stained

with ethidium bromide and visualized under UV illumination [30]. e

percentage of polymorphism was calculated using following equation:

Percent polymorphism=A/B × 100

Where, A=Number of polymorphic bands in treated sample; and

B=Number of polymorphic bands in control.

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from N. otitidis cells (set A, sample coded

as 9A) using genomic purication kit, according to the manufacturer

instructions. 16S rDNA gene (~ 1.5 kb) fragment was amplied with the

help of high-delity polymerase chain reaction (PCR) using universal

reverse primer (3' -ACGGTCATACCTTGTTACGACTT-5' ). Amplied

products were subjected to gel electrophoresis in 1.0% agarose gel,

stained with ethidium bromide and visualized under UV light in a gel

documentation unit (BioRad Laboratories, USA). e PCR amplied

fragment was puried from the agarose gel using a DNA gel extraction

kit. Sequencing of amplied product was done on a commercial basis

from Bangalore Genei, India. e 16S rDNA sequences obtained were

aligned and compared with the sequences stored in GenBank database

available from National Center for Biotechnology Information (NCBI)

using the algorithm BLASTn program. Multiple sequence alignment/

phylogenetic tree were established using MEGA3.1 molecular soware

Antimicrobial susceptibility test

Antimicrobial susceptibility pattern of Nocardia can vary from

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Mondal SC, et al. (2015) Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

species to species. e therapeutic eectiveness depends on the proper

identication of species in infected patients and on in vitro sensitivity

studies [32]. e antimicrobial sensitivity assay against Nocardia

species had been especially considered in refractory cases. A standard

test for antimicrobials sensitivity assessed by broth micro dilution and

with cation-supplemented Mueller-Hinton broth has been approved by

the National Committee for Clinical Laboratory Standards (NCCLS)

[29]. e outcome of N. otitidis susceptibility pattern and MIC values of

tested antimicrobials aer bioeld energy treatment are summarized in

Table 1 and 2 respectively. e data were analyzed and compared with

respect to the control. Study was carried out in twelve antimicrobials

for assessment of antimicrobial susceptibility and MIC value. e

treated cells of N. otitidis did not show any alteration with respect to

antimicrobial susceptibility pattern as compared to the control (Table

1). e susceptible nature of N. otitidis to amikacin, sulfamethoxazole,

and ciprooxacin and resistance pattern to ceriaxone, amoxicillin-

clavulanic acid, and imipenem were well corroborated with the

literature data [3]. Beside this, the MIC value of trimethoprim/

sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 µg/mL)

in bioeld energy treated sample as compared to the control sample.

Moreover, the MIC value was slightly reduced (>32/16 to 32/16 µg/mL)

in amoxicillin/k-clavulanate aer the bioeld therapy. Overall, 16.67%

out of twelve antimicrobials showed an alteration of MIC values. Rest

of the antimicrobials did not show any alteration of MIC values with

respect to the control sample. Based on the literature, a combination

of sulfa and antimalarial drugs, i.e., trimethoprim/sulfamethoxazole

are the drug of choice against Nocardia infections as compared to the

single sulfa drug [11,12]. In this experiment, the susceptibility nature

of trimethoprim/sulfamethoxazole was constant in both controls as

well as in treated sample, while the MIC value was reduced by two-

fold in treated sample aer Mr. Trivedi's bioeld energy treatment.

Hence, authors assumed that this improvement of MIC value without

alteration of sensitivity pattern may be due to the eect of putative

energy transmit through bioeld healing (Table 2).

Random amplied polymorphic DNA (RAPD) analysis

e treated and control samples were identied on the basis of

their dierent and discriminative RAPD patterns. RAPD is a preferred

tool that is being used now days to correlate the genetic similarity

or mutations between species. e simplicity and wide applicability

of RAPD analysis mainly depend upon the use of short nucleotide

primers, which were unrelated to known DNA sequences of the target

organism [33]. DNA polymorphisms can be eciently detected using

the PCR primers and identify inter-strain variations among species in

treated samples [34]. e degree of relatedness and genetic mapping

can be correlated between similar or dierent treated sample species

e DNA ngerprinting by RAPD analysis using ve primers was

carried out on the control and treated samples. DNA ngerprinting by

RAPD analysis of the control and treated samples are shown in Figure 1,

and the polymorphic bands are marked by arrows. e RAPD patterns

of treated samples showed some unique and dissimilar patterns. DNA

polymorphism analyzed by the RAPD analysis was presented in Table 3.

e level of polymorphism between control and treated samples (A, A1,

B, and B1) are summarized in Table 4. e level of polymorphism was

found in an average range of 34 to 53% in treated samples as compared

to control in N. otitidis aer the bioeld treatment. e highest change

in DNA sequence was observed in treated groups with RBA 13A primer

as compared to the control; a negligible change was found in treated

group with RBA 8A primer as compared to the control (Table 3 and 4).

e bacteria that are poorly dierentiated by conventional methods

needs molecular analysis method like 16S rDNA sequence [36]. is

molecular-based technique is a suitable tool for identication of most

Figure 1: Random amplied polymorphic-DNA fragment patterns of Nocardia

otitidis generated using ve RAPD primers, RBA 8A, RBA 13A, RBA 20A, RBA

10A and RBA 15A. 1: Control; 2: Treated A; 3: Treated A-1; 4: Treated B; 5:

Treated B-1; M: 100 bp DNA Ladder.

11. Amoxicillin/k-clavulanateR R

12. Trimethoprim/sulfamethoxazoleSS

R: Resistant; S: Susceptible; Control: ATCC strain of N. otitidis (without bioeld

energy treatment); Treatment: ATCC strain of N. otitidis (with Mr. Trivedi's bioeld

Table 1: Antimicrobial susceptibility pattern of antimicrobials against ATCC strain of

Nocardia otitidis after bioeld treatment on day 10 as per CLSI guidelines.

2. Clarithromycin>32.0>32.0

10. Gatioxacin 0.12 0.12

11. Amoxicillin/k-clavulanate>32/16 32/16

12. Trimethoprim/sulfamethoxazole0.5/9.50.25/4.75

CLSI: Clinical and Laboratory Standards Institute; MIC values are presented

as µg/mL; Control: ATCC strain of N. otitidis (without bioeld energy treatment);

Treatment: ATCC strain of N. otitidis (with Mr. Trivedi's bioeld energy treatment)

Table 2: Minimum inhibitory concentration (MIC) of antimicrobials against ATCC

strain of Nocardia otitidis after bioeld energy treatment on day 10 as per CLSI

guidelines.

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Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis - PdfSR.com

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

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	 Antimicrobial Susceptibility Testing of Burkholderia Cepacia
	

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	 Antimicrobial Susceptibility Testing of Burkholderia Cepacia
	

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| Burkholderia cepacia antimicrobial susceptibility testing was conducted using MicroScan Walk-Away®. The human energy treatment on MDR strain of B. cepacia showed a significant change in sensitivity pattern of different antimicrobials.

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phenotypic characteristics of b. cepacia

# data[].attributes.attachment_link.title
"An Effect of Biofield Treatment on Multidrug-resistant Burkholderia cepacia: A Multihost Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B.

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		 Energy Healing Impact on Biotype Number of B. Cepacia
	

# data[].attributes.subject
	
	
	
	
	
		 Energy Healing Impact on Biotype Number of B. Cepacia
	

# data[].attributes.body
| The present study was aimed to evaluate the impact of biofield treatment on antimicrobial susceptibility pattern, MIC values, biochemical reactions and biotype number of MDR strain of B. cepacia. Read here the complete study paper.

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		 Burkholderia Cepacia Biochemical Characteristics
	

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		 Burkholderia Cepacia Biochemical Characteristics
	

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| The findings of the present study suggest that the unique energy treatment has a significant effect on the different biochemical characteristics of multidrug resistant strain of B. cepacia.