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Amber Montoya
3rd Period
March 15 2022

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DNA, also known as Deoxyribonucleic Acid are two long strands in the form of a double helix that contains our genetic code. Our genetic code are essentially a set of rules used by our cells to convert the instructions in DNA to what we need in life and proteins. DNA is made up of nucleotides that are the building blocks of DNA and made up of phosphates, sugars, and nitrogen bases. These nitrogen bases have rules called Chargaff's rules, where A=T (Adenine = Thymine) and C=G (Cytosine = Guanine).  Connecting these bases are hydrogen bonds.

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Strawberry Extraction DNA Lab : Intro Continued 

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Strawberry Extraction DNA Lab : Intro Continued 

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Strawberry DNA Extraction : DNA/RNA T-Chart 

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Strawberry DNA Extraction : DNA/RNA T-Chart 

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Strawberry DNA Extraction Lab : Materials and Pictures 

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Strawberry DNA Extraction Lab : Materials and Pictures 

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GOGGLES

Ziplock bag

A strawberry

10 mL DNA extraction buffer

Cheesecloth

Funnel

Test tube

test tube stand

Stir stick

Ethanol

Paper towels

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Strawberry DNA Extraction Lab : Procedures and Pictures 

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Strawberry DNA Extraction Lab : Procedures and Pictures 

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1. Place one strawberry in a Ziploc bag.

2. Smash/grind up the strawberry using your fist & fingers for 2 minutes. Careful not to break the bag!!

3. Add the provided 10 mL of extraction buffer (salt & soap solution) to the bag. (DNA Extraction Buffer:1000 ml of deionized water, 50 ml of dish soap, 1 teaspoon of salt)

4. Gently kneed/mush the strawberry in the bag again for 1 minute.

5. Assemble your filtration apparatus as shown in the diagram.

6. Pour the strawberry slurry into the filtration apparatus & let it drip directly into your test tube … only until your test tube is ½ full.

7. Slowly pour cold ethanol into the tube.

8. Dip the stir stick into the tube where the strawberry extract and ethanol layers come into contact with each other.

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Strawberry DNA Extraction Lab : Data Analysis Paragraph 

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Strawberry DNA Extraction Lab : Data Analysis Paragraph 

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To begin, we put the strawberry in the Ziplock bag and began mush the strawberry for a few minutes to break open the cells. Then we mixed the strawberry mush with salty/soapy solution, mushing it again for 2 minutes to break up proteins and dissolve cell membranes. Continuing that, we then filter strawberry slurry through cheesecloth to separate the components of the cell. Lastly, we pour the mush into the test tube half way then begin to add ethanol to filtered extract to precipitate DNA from the solution.

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Strawberry DNA Extraction Lab : Extension Questions 

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Strawberry DNA Extraction Lab : Extension Questions 

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To sum up our strawberry lab, we crushed the strawberry in a Ziplock bag, added soapy solution and crushed again. Then we added the mushy substance to an apparatus that filtered the strawberry chunks versus the actual juice. With that juice, we added it to a test tube then added the ethanol, where the DNA slowly came up into the ethanol. I learned that plants and veggies, like all other living things, have DNA. If I did this experiment again, I'd probably change out the cheese cloth because it was kind of hard for the mushy juice to separate from the strawberry chunks. Now that I know you can extract DNA from a strawberry, I'd wanna see how the DNA looks under a microscope, whether it looks the same as ours or not. Two traits expressed in a strawberry are color and size.