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A connection to the real world is that long ago, around the same time the book was written, people with different skin tones were discriminated against.
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Another connection is that women back then were also not allowed certain jobs and they were treated like property.

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She feels worthless and useless so she wants attention but she is judged for trying. ------>"She ain't concealin' nothing. I never seen nobody like her. She got the eye goin' all the time on everybody. I bet she even gives the stable buck the eye. I don't know what the hell she wants."
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She just wants someone to talk to but Curly doesn't want anyone to even look at her and that makes her feel even worse. She is lonely and she can't do anything to make her feel better.----> "Wha's the matter with me?" she cried. "Ain't I got a right to talk to nobody? Whatta they think I am, anyways? You're a nice guy. I don't know why I can't talk to you. I ain't doin' no harm to you."

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"'Cause I'm black.  They play cards in there, but I can't play because I'm black.  They say I stink.  Well, I tell you, you all of you stink to me."
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"This is just a nigger talkin', an' a busted-back nigger. So it don't mean nothing, see?" -----> no one cares about what he thinks just because he's black.
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"…You go on get outta my room. I ain’t wanted in the bunk house, and you ain’t wanted in my room."
"Why ain’t you wanted?" Lennie asked.
"’Cause I’m black…"

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People exclude others who are different because they think they don't deserve the same things if they are not the same, but the truth is we are all different in our own ways and everyone deserves a chance.

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Curly's wife is excluded from a lot of things because she is a woman. She stays alone for most of the day and when she does go out, she is judged by everyone because she is overly friendly with the men. She just wants attention but she can't get it without flirting or dressing really nicely.

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Crooks is marginalized in the book. He is not allowed to sleep with the others, he eats alone, he doesn't talk to others and he works more than the others. He is treated unfairly and he has to live in bad conditions.

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Marginalization
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People tend to marginalize others who are different.