# data[].attributes.body
What are the uppstream processes for bacterial cells which produce enzymes?

# data[].attributes.body
What is the safest way to thaw the cells?

# data[].attributes.body
In which cases is the HTST method used

# data[].attributes.headline
How big a part of the whole process is the pretreatment?

# data[].attributes.subject
How big a part of the whole process is the pretreatment?

# data[].attributes.body
What's the most common method of sterilization when dealing with mammalian cells

# data[].attributes.body
What is the most cell-friendly way to melt the cells
?

# data[].attributes.body
Can pure oxygen be used instead of filtered air and what are the costs?

# data[].attributes.body
What is the temperature during the sterilization of a bioreactor?

# data[].attributes.body
Is it necessary to remove the foreign cell material e.g. endotoxins after sterilization?

# data[].attributes.body
Which of the sterilisation systems are the most effective, steam, heating or filttion?

# data[].attributes.body
What is the optimal temperature if you are using the heating method for sterilizing ?

# data[].attributes.body
Which is the correct term when talking about cell suspension: melt or thaw?

# data[].attributes.headline
Can you show an example of a medium preparation and optimization process? What are the main arts to master?

# data[].attributes.subject
Can you show an example of a medium preparation and optimization process? What are the main arts to master?

# data[].attributes.body
How do you prepare a medium for mammalian cells on industrial scale?

# data[].attributes.body
What is the optimal pH for a mammal sterilisation?

# data[].attributes.body
How long will the cells survive in frozen state?

# data[].attributes.headline
Why are antibiotics added to the culture medium?

# data[].attributes.subject
Why are antibiotics added to the culture medium?