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Remove "all of the above" as a response; adjust responses to similar length; remove negative phrasing; shorten stem

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Remove "all of the above" as a response; adjust responses to similar length; remove negative phrasing; shorten stem

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Which of the following describes the reasoning behind allowing a region of approximately (~) 100 bp between the primer binding region and the target region during primer design for Sanger sequencing?

A. Chain termination occurs after ~100 bps have been incorporated.

B. DNA polymerase preferentially incorporates ddNTPs for the first ~100 bps.

C. Signals from products shorter than ~100 bps are difficult to detect.

Correct answer: C

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Consider some of your own test items.

Use the item writing guidelines and work to improve TWO of your items.

Under your name (Q1 & Q2), post your original question and then copy and paste the revised question from Google Docs.

The Feedback column will be empty.

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Click on the (+) symbol.
You can copy your text in the box and even add images. (use the up arrow below to add images to your question

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You found a lizzard with a cut tail. You pick it up at home to help it recover. After some days you realize the tail is fine. Among all processes, what is the process by which tail regenerated?

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You found a lizzard with a cut tail. You pick it up at home to help it recover. After some days you realize the tail is fine. Among all processes, what is the process by which tail regenerated?

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Which macromolecules are part of the central dogma in biology

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Which macromolecules are part of the central dogma in biology

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Which of the following components is NOT required for both Gibson assembly and forced cloning?

a. Linearized plasmid

b. Ligase

c. Exonuclease

d. Amplified insert

Correct answer: c

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Answer true or false to this statement for each option below. This component is required for both Gibson Assembly and forced cloning.
a. Linearized plasmid

b. Ligase

c. Exonuclease

d. Amplified insert

Correct answer: a-True, b-True, c- False, d- True

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When designing a primer for Sanger sequencing, it is recommended that you allow a region of approximately 100 bp between the primer binding region and the target region for high quality sequencing data. Which of the following accurately describes the reasoning behind this consideration during primer design?
A. Chain-termination does not occur for approximately 100 bps, after which fluorescence signals are produced.

B. DNA polymerase preferentially incorporates only ddNTPs for the first 100 bps.

C. Signals from short products are difficult to detect resulting in poor quality of base-calling in the chromatogram.

D. All of the above.

Correct answer: C

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These revision seem very helpful in considering the cognitive load required to process the question.
Great job!

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Would you expect this to be a selected response question or essay?

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Could you include a diagram that depicts the relationship of energy and nutrients for the possible answers. I initially think of differently shaded of arrows pointed in similar or opposite directions but it may not completely fit the concept.

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4. Which statement best describes the interaction of energy and nutrients within an ecosystem?

a. Both energy and nutrients cycle through biotic, but not abiotic, components.

b. Energy cycles through biotic components; nutrients cycle through abiotic components.

c. Both energy and nutrients travel throughout ecosystems, but they follow separate, disconnected routes.

d. Energy and nutrients enter biotic components through primary producers; energy flows one way and nutrients cycle.

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This is a good change to avoid the negative tense when asking a question.

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You could try an ordering question (with careful phrasing)- put the three macromolecules in order of typical...

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Really good similar sentence structure between a & b and c & d, so you could do an either/or here instead of 4 choices so students don't get overwhelmed with reading so many responses. Good to test vocabulary at same time (if aligns with learning outcomes)