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Testing for the gasses evolved from the hydrogen peroxide

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The rat has been dead for 14 days

All cells in the rat is dead and all cell activity should be stopped

Take tissue from the liver and add the sample to the beaker containing hydrogen peroxide

Whit gass bubble evolves from the beaker rapidly.

The gas bubble can relight a glowing splint

This prove that the enzymes in the tissue is still functioning

Because all living things in the rat should be dead and no cellular activity can be carry out

Therefore the enzymes is not living

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The following experiment was conducted to find out if enzymes are non-living.

A piece of liver tissue was added into the hydrogen peroxide solution.

Colorless bubbles evolved as liver contains the enzyme catalase, which catalyses the breakdown of hydrogen peroxide into oxygen.

To further test if the gas is oxygen, a glowing splint was exposed to the gas in the beaker. Since the glowing splint relighted, the gas is oxygen.

A glass lid was used to cover the beaker to prevent the gas evolved from excaping

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Liver

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The rat has been dead for a few week, this means that the tissues and organs of the rat are dead.

First and foremost, liver tissues should be extracted.

Secondly, hydrogen peroxide should be prepared.

Thirdly, liver tissues should be immersed in hydrogen peroxide.

Lastly, if colorless bubbles evolve, test if the gas relight a glowing splint, it means that the gas evolved is oxygen.

this means that the breakdown of hydrogen peroxide into oxygen is catalysed by catalase from liver.

This indicates that even when all the living tissues and organs died, the enzymes can continue to work. This can prove that the enzymes are non-living.

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Liver

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Enzymes are found in living organism. However, it is also found in industrial products. What do you think? How do you verify your hypothesis?