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Researching the efficacy of digestive supplements/over the counter drinks that boost digestive system and comparing them among each other

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Researching the efficacy of digestive supplements/over the counter drinks that boost digestive system and comparing them among each other

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something in the field of medicine, pharmaceutical, human behavior, public health

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something in the field of medicine, pharmaceutical, human behavior, public health

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Rough draft of project proposal.

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E. Coli - accepted
Lactobacillus - submitted
Kombucha - submitted
Apple Cider Vinegar - submitted
Swartz Probiotics - submitted

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Final draft of project proposal.

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October 22: make procedures and finish planning

October 29: grow Lactobacillus and maybe E.Coli

November 5: Begin Project

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Make sure you guys are adding to this each week! You could add photos of the MRS you made last week!

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Make sure you guys are adding to this each week! You could add photos of the MRS you made last week!

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Today we faced many issues with our original procedures so the new procedures that will work are below.

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Today we faced many issues with our original procedures so the new procedures that will work are below.

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1. Sterilize 20 test tubes and all beakers that will be used

2. Label 20 test tubes appropriately

3. Add 4 mL of MRS into 10 test tubes

4. Add 4 mL of LB into the remaining 10 test tubes

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1. Heat up 200 mL of water on hot plate to 30 degree

2. add one pill in the water and turn on stir rod

3. Wait until completely dissolved

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1. Measure 200 mL of DI Water and add to sterilized beaker

2. Measure 100 mL of Kombucha and add to the beaker with water

3. Stir until solution is thoroughly mixed

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1. Measure 200 mL of DI Water and add to sterilized beaker

2. Measure 100 mL of Apple Cider Vinegar (ACV) and add to the beaker with water

3. Stir until solution is thoroughly mixed

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1. Add 10 micro liters of lactobacilli to 10 MRS

2. Add 10 micro liters of E. coli to 10 LB

3. Stir each up and see if need to add more according to whats leftover

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1. Add 1 mL of water to 4 tubes

2. Stir Kombucha and add 1 mL to 4 tubes

3. Stir ACV and add 1 mL to 4 tubes

4. Stir dissolved probiotic solution and add 1 mL to 4 tubes

5. Lastly, cover each test tube with parafilm and put in around 30 degree

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This is the diluted Kombucha and ACV made in lab

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Today we wrote our recipe-like procedures in our lab notebooks.

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Today we wrote our recipe-like procedures in our lab notebooks.

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Our final orders came in and we are on track with our timeline to start the project next week on October 29th. 

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Our final orders came in and we are on track with our timeline to start the project next week on October 29th. 

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The google doc has the links to all of the articles used in our research.

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The google doc has the links to all of the articles used in our research.

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All items are now available

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This is the probiotic pill the first time that we tried to dissolve it-- it clumped up and didn't dissolve.

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Eventually with heat, stirring, and much more water, we got the probiotic pill to dissolve (pictured below).

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We have noticed the large areas that could be affected by human error.

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We have noticed the large areas that could be affected by human error.

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1. The test tubes and beakers can very easily become unsterile

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1. The test tubes and beakers can very easily become unsterile

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2. The measured amounts of liquid are not always equal because we had to put in 1 mL 10 times to each test tube.  

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2. The measured amounts of liquid are not always equal because we had to put in 1 mL 10 times to each test tube.  

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- First, our probiotic pill did not dissolve in water as we thought that it would, so we had to work with it and find a way to force it to dissolve (we eventually got it to dissolve with continued stirring and heat)
-We also used unsterile test tubes by accident, so after we filled them all with MRS broth and LB broth, we had to dump them out and start all over.

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liquid nutrient broth (MRS- 10 test tubes)
1. combine 55g of medium with one liter of demonized water
2. stir to mix throughly
3. boil to dissolve completely (do not overheat)
4. auto clave at 121C for 15 minutes

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1. put 4 mL of the MRS broth in ten test tubes
2. put 4 mL of the LB broth in ten test tubes
3. place caps back on test tubes to make sure they stay sterile
4. make the probiotic pill solution (info on precious lab progress column)
5. add the solutions to the test tubes
6. put in incubator
7. store in warm place

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We successfully conducted the experiment. 

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We successfully conducted the experiment. 

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We followed all of the steps in the procedures, and left the tubes in the 37 degree incubator.

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solutions under the flame

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l bacillus

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Today we measured the test tubes in the spectrophotometer 

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Today we measured the test tubes in the spectrophotometer 

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Just by looking at these pictures, it is easy to see the difference in clarity in the tubes before we even used the spectrophotometer.