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The first mini threshold is photosynthesis, it is likely that during this threshold the first prokaryotes evolved deep in the ocean. Around 3.5 billion years ago some cells migrated to the surface of the ocean and evolved to use more abundant energy from the sun. This process was named and is still named photosynthesis.

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These are more complex
cells whose DNA is locked up inside a special case
called the nucleus, which helps protect and preserve
vital genetic information. Eukaryotes also contain
tiny organs called organelles. Like the organs in
your body, they perform special functions such as
photosynthesis, or processing oxygen.

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Mini Threshold 3 - Multicellular Organisms 

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Mini Threshold 3 - Multicellular Organisms 

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Multicellular organisms, specialized organelles came together to form more complex eukaryotes, different eukaryotes came together
to form even more complex life forms. These
organisms could contain billions of cells, each with
a different function, but all sharing the same DNA
so they worked together. With networks of specialized cells and cooperation, multi celled organisms
could respond to changes in the environment in
entirely new ways, further developing a key survival trait of life that we call homeostasis.

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Multi Celled organisms needed a way to coordinate all the activities going on inside them and this
became the work of special nerve cells. In some
organisms these cells began to cluster at the head
and down the spinal cord to form the first brains.
Organisms with brains could process much more
information, and they could react to it in even more
complex ways, which enabled richer and more
sophisticated activities, like thinking, and perhaps
even consciousness.

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From about 475 million years ago, some multicellular organisms, beginning with plants and fungi, left the oceans for land. There was a great incentive, this new environment was rich in new opportunities for organisms that could find ways to survive. That was a challenge, though, as these organisms had to develop special skins to avoid drying out, special ways to breathe out of water, and new ways to reproduce.

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The first animal
to live on land may have been like a modern lungfish, but other land animals soon evolved, such as
amphibians. These still needed to return to the water
to reproduce. Then came reptiles like crocodiles or
dinosaurs. These developed large, leathery eggs that
could survive well away from water. Then about 250
million years ago, the first mammals appeared on
Earth evolving from a branch of reptiles that loosely
resemble birds. Mammals are warm-blooded,
they’re furry, and they don’t lay eggs, and you and I
are mammals.