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Photo Example of a Transmission Electron Microscope

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Photo Example of a Transmission Electron Microscope

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TEM releases a broader beam of electrons to penetrate the specimen, resulting in a visualization at a high magnitude.

source: https://www.technologynetworks.com/analysis/articles/sem-vs-tem-331262

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Transmission Electron Microscopes (TEM) use rays of electrons that go through the sample. These electron microscopes show highly detailed 2D images of tiny cell structures, important in cell biology. The samples are usually thin slices of dead specimen, and require elaborate preparation. These resulting images are colorless, as they use electrons and no other dye additive for differentiation.

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Photo Example of an Image from a Transmission Electron Microscope

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Photo Example of an Image from a Transmission Electron Microscope

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Photo Example of a Scanning Electron Microcope

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Photo Example of a Scanning Electron Microcope

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Structurally, SEM releases a fine beam of electrons which scans the sample.

source: https://www.technologynetworks.com/analysis/articles/sem-vs-tem-331262

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Scanning Electron Microscopes (SEM) use unconcentrated electrons by scattering them onto the surfaces of samples. These electron microscopes create a 3D visualization of the structure, recording composition and topography information useful in knowing for cell biology. Images from SEM will end up colorless much like TEM due to electrons being used instead of light. The samples also must be dead and require extensive preparation.

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Photo Example of Images from a Scanning Electron Microscope

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Photo Example of Images from a Scanning Electron Microscope

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Photo Example of a Fluorescent Microscope

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Photo Example of a Fluorescent Microscope

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Fluorescent microscopes release light to brighten up the image displayed.

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Fluorescent microscopes use a type of fluorescent dye to stain the slides it is viewing. They are a type of light microscope. The light from the microscope then reflects onto the slide allowing for the details to be observed. The images observed are usually in color and 2D. There some subcategories of fluorescent microscopy, such as like superresolution fluorescent microscopy (can observe samples at a smaller level), and confocal fluorescent microscopy (creates images by scanning a beam of light across a sample stained with fluorescent dye).

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Photo Example of an Image from a Fluorescent Microscope

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Photo Example of an Image from a Fluorescent Microscope