# data[].attributes.headline
	
	
	
	
	Biofield | Potential Impact on Human Brain Tumor Cells
	

# data[].attributes.subject
	
	
	
	
	Biofield | Potential Impact on Human Brain Tumor Cells
	

# data[].attributes.body
Research done by Mahendra Kumar Trivedi - The aim of present study was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.

# data[].attributes.attachment_link.title
The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy | Mahendra Trivedi | Academic Room

# data[].attributes.attachment_link.description
Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non- GBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedi's biofield treatment for the assessment of its potential.

# data[].attributes.headline
	
	
	
	
	Biofield Tretatment on Raoultella ornithinolytica | CiteSeer
	
 

# data[].attributes.subject
	
	
	
	
	Biofield Tretatment on Raoultella ornithinolytica | CiteSeer
	
 

# data[].attributes.body
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. Read this study to investigate the impact of biofield tretatment on raoultella ornithinolytica.

# data[].attributes.headline
	
	
	
	
	Biofield Therapy Evaluation on Properties of Cadmium Powder
	

# data[].attributes.subject
	
	
	
	
	Biofield Therapy Evaluation on Properties of Cadmium Powder
	

# data[].attributes.body
The study conducted by Mahendra Trivedi, to evaluate the biofield therapy impact on thermal, physical and structural properties of cadmium powder.

# data[].attributes.headline
	
	
	
	
	Potential Impact of Biofield Treatment on Human Brain Tumor Cells
	

# data[].attributes.subject
	
	
	
	
	Potential Impact of Biofield Treatment on Human Brain Tumor Cells
	

# data[].attributes.body
The human brain tumor, GBM cultured cells were divided into two groups, GBM control & treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non- GBM control and non-GBM treatment. Read the complete study to understand it in detail.

# data[].attributes.attachment_link.readable_text
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Trivedi et al., J Integr Oncol 2015, 4:3

http://dx.doi.org/10.4172/2329-6771.1000141

ISSN: 2329-6771 JIO, an open access journal Volume 4 * Issue 3 * 1000141

Keywords: Glial cell; Glioblastoma; Brain tumor; Bioeld treatment;

Time-lapse video microscopy

Brain tumors are typically very heterogeneous, aggressive

neoplasms at the cellular level which aects both children and adults

[1]. Statistics based on cancer research institute explore that 1 in 161

individuals born today with brain cancer at dierent point of life. In the

U.S., 22,850 men and women are diagnosed with brain cancer every year,

and 15,320 deaths are caused by this disease [2]. As per World health

organization classication, glioblastoma (GBM) is also known as grade

IV astrocytoma [3]. GBM is one of the most malignant form of human

brain tumor. e mean survival time for GBM patients is approximately

12 months [4]. Time-lapse video microscopy is a technique that aids to

assess cellular behavior in real time. is technique is used to collect

a two dimensional image data at dierent time intervals. en, these

data are converted to make a movie. is unique technique is used

in various elds of cancer and stem cell biology for assessment of

cell translation, division, and death [5,6]. Presently, several strategies

are available for the treatment of GBM such as surgical techniques,

radiotherapy, chemotherapy and electrochemotherapy [7]. Apart

from commonly used chemotherapeutic agents (such as nitrosoureas

derivatives, platinum based drugs, and taxol) and gene therapy, as

a novel therapeutic modality frequently being used in cancer [8].

However, chemotherapy suers several major drawbacks such as all

tumors are not responsive to chemotherapeutic agents to some extent,

incidence of bone marrow cells damage, amenorrhea, alopecia, sexual

dysfunction and adversely aect the quality of life of patients [9].

Malignant brain tumors like GBM are very dicult to treat

now a days. e diculties to treat against GBM are due to lack of

proper preventive strategies, and unavailability of practical method

for screening [10]. erefore, new, more eective and better tolerated

anti-tumor drugs or some alternative treatment strategy are needed.

Based on the above lacunas an alternative way which may be useful to

kill or eradicates the proliferative tumorous cells and simultaneously

defense the normal brain glial cells. Bioeld treatment is an alternative

approach which may be useful to improve these unlled spaces

associated with cancer patients. e human bioeld is the energetic

matrix that surrounds the human [11]. It directly links with the cellular

activity that allows the DNA to communicate faster than light and

maintain intelligence in the organisms [12].According to universal

principles of Maxwell's equations and principle of reciprocity denes

electromagnetic connections related to human bioeld [13]. us, a

human has ability to harness the energy from environment/Universe

and can transmit into any object (living or non-living) around the

Globe. e object(s) always receive the energy and responded into

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research

Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City,

Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-

Received July 07, 2015; Accepted July 21, 2015; Published July 28, 2015

Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential

Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-Lapse Video

Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The

aim was to evaluate the impact of bioeld treatment potential on human GBM and non-GBM brain cells using two

time-lapse video microscopy technique.

Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM

treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non-

GBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedi's bioeld

treatment for the assessment of its potential. Two time-lapse (10 hours prior; 10 hours after) video microscopy

experiment was performed on tumor and non-tumor brain cells in six replicate (n=6). For each microscopic eld, the

total cell number was counted and each cell was tracked over the 20 hours period. The potential impact of bioeld

treatment was assessed by comparing cell death rate in both GBM and non-GBM cells before and after bioeld

treatment.

Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the bioeld

treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death

rate was exponentially increased (41%) after bioeld treatment as compared to control. The treated non-GBM

cultured cells showed a signicant reduction (64%) of cell death rate i.e. protective effects as compared to non-GBM

control.

Conclusion: Altogether, data suggests that bioeld treatment has signicantly increased the cell death rate of

treated GBM cells and simultaneously boost the viability of normal brain cells. Therefore, bioeld treatment could be

a suitable alternate treatment strategy for cancer patients in near future.

The Potential Impact of Biofield Treatment on Human Brain Tumor Cells:

A Time-Lapse Video Microscopy

Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Sambhu Charan Mondal2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India

Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-

Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141

ISSN: 2329-6771 JIO, an open access journal Volume 4 * Issue 3 * 1000141

useful way that is called bioeld energy. is process is known as bioeld

treatment. e bioeld can be monitored using electromyography,

electrocardiography and electroencephalogram [14]. Mr. Mahendra

Trivedi's unique bioeld treatment (e Trivedi Eect) has been

well known for altered characteristics features of microbes [15-17],

improved the overall productivity of crops [18,19], and also transform

the structural, physical and chemical properties of materials [20-23].

Exposure to bioeld energy caused an increase in medicinal property,

growth, and anatomical characteristics of ashwagandha [24].

On the basis of above facts and literature, present study was

undertaken to evaluate the impact of Mr. Trivedi's bioeld treatment

on human GBM brain tumor cells.

Human U87 glioblastoma cells were received from California

pacic medical center research institute, bioscience laboratory, San

Francisco, CA. e cultures were prepared and maintained as per the

protocols.

Study design and bioeld treatment strategy

e GBM cultured cells were divided into two groups viz. GBM

control and GBM treatment. Besides this, normal brain cultured cells

were also divided into two groups such as non-GBM control and

non-GBM treatment. Prior to experiment, each cell culture plate was

prepared with six replicates (n=6) of both GBM and non-GBM cells.

Each cell culture plate was grown side-by-side in sub-compartments of

one culture plate, so that both types of cells were exposed to treatment

and without treatment simultaneously. One set of culture plates (GBM

and non-GBM treatment) were handed over to Mr. Trivedi for bioeld

treatment under laboratory condition. Mr. Trivedi provided the

treatment through his energy transmission process to the culture plates

in front of the time-lapse microscope to deliver bioeld treatment from

outside a plexiglass environmental chamber attached to the microscope

without touching them. Another set was considered as control

experiment in which no treatment was delivered. Aer treatment, all

the culture plates were analyzed for assessment of bioeld treatment

potential. In brief, during the experiment involving bioeld treatment,

Mr. Trivedi was escorted to the laboratory and seated a short distance

(approximately 20 cm) from the time-lapse microscope (Figure 1).

During the control experiment, the cells were handled identically but Mr.

Trivedi did not visit the laboratory. A laboratory technician placed the

cell culture plates inside of an environmental chamber for examination

by time-lapse microscopy without knowledge of the eventual treatment

condition for the samples. e following day, laboratory technician has

converted the acquired images into Quicktime movies and transferred

the movies to a database using blinding codes for analysis.

Two time-lapse video microscopy technique

Two time-lapse video microscopy experiments were followed in

human brain tumor GBM cells and normal brain cells for 20 hours time

cycle. Microscopic images were acquired in every 5 minutes interval,

from 10 hours prior and 10 hours following the treatment time i.e. 20

hours for each experiment, and compiled into time lapse videos. Figure

2 depicts the time schedule for two time-lapse video microscopy of cell

Criteria for analysis of cell count

For each microscopic eld, the total number of cells were counted

and each cell was tracked over the 20 hours period. Cells that entered

the microscopic eld aer the initial frame were neither included,

nor identied as dead at the start of the video. Cell death, divisions

and emigrations (cells migrating out of the microscopic eld) were

counted for varying numbers of cells and count was made every single

hour. Aer that, all the data were collected and entered into excel

spreadsheets, the blinding codes were revealed and the results were

sorted according to experimental group for analysis.

Since, brain tumors especially GBM are the most critical medical

problem aecting major part of population, physician suggests

chemoprevention along with radiation strategies to reduce the

incidence and mortality rate. However, there are scientic evidence and

large number of in vitro studies already reported for bioeld treatment

and its eect in cancer cells [26,27]. Previous studies indicated that

bioeld energy showed an inhibitory eect on metastasis and immune

responses in cancer cell models [11,26,28].

An exponential growth model was applied to the study data derived

through time-lapse video microscopic technique which aims to take

into account potential inuence on cell death rates due to change the

number of cells in each microscopic eld throughout the observation

period. Death counts during 10 hours period of observation were

converted to rates per cell by the following formula:

Death rate = ln(N(t-1)) - ln(N(t-1) - d(t))

Where, N(t-1) is the number of cells at the start of the time period

and d(t) is the number of deaths during the time period. ese estimates

Figure 1: Schematic diagram represents the physical arrangement of bioeld

treatment session from a short distance (approximately 20 cm), outside a

plexiglass environmental chamber attached to the microscope.

Figure 2: Schematic diagram represents the schedule for time-lapse video

microscopy of cell cultures, A) treatment and B) control.

Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-

Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141

ISSN: 2329-6771 JIO, an open access journal Volume 4 * Issue 3 * 1000141

very popular and scientically studied [31,32]. erefore, Mr. Trivedi's

bioeld treatment could be an eective (with no existing side eects),

cost eective (no drugs were used) and alternate approach for cancer

chemotherapy. Research on bioeld treatment and its impact on cancer

growth has been continuously studied by researchers, which might

works as alternate and complementary medicine to prolong the life of

cancerous patients along with quality life.

Overall, the study data indicates that bioeld treatment has

signicantly increased (41%) the death rate of GBM brain tumor

cells as compared to untreated GBM cells. Besides this, non-GBM

treatment cultured cells showed a signicant reduction of cell death

rate (64%) as compared to normal brain cells (Figure 5). Based on the

obtained results, it is expected that bioeld treatment could be novel,

cost eective and an alternative advanced strategy towards brain tumor

In conclusion, the study results suggest that the bioeld treatment

has signicantly increased (41%) the cell death rate of GBM treatment

brain tumor cells, as compared to GBM control cells. In addition,

bioeld treatment also showed a signicant reduction of cell death

rate (64%) in non-GBM treated culture cells i.e. protective eect as

compared to non-GBM brain cells. Based on the results, the inexpensive

are based on an assumption that counts are dependent on the number

e potential impact of bioeld treatment on GBM brain culture

cells was assessed by comparing cell death rates before and aer

treatment. In non-GBM (control) group, values for cell division

were not signicantly analyzed due to very low cell divisions rate, as

the majority of the cell counts being zero throughout the 20 hours

observation period. is lack of cell division under control conditions

for the normal brain cells indicated that the culture conditions were

not sucient to keep the normal cells viable throughout the extended

time in culture. is is oen may be the case with cultures of normal

cells, whereas cancerous cells typically survive in culture more readily.

Comparing the total number of cell deaths over the 10 hours prior

and 10 hours following the treatment time (T = 0) for the four groups of

cells and dividing these values by the initial cell counts for each group

gives a rough estimation of the potential eect of treatment. Analysis

using the exponential growth model indicates that the cell death rate

in GBM treatment cultured cells increased aer bioeld treatment

however, no change in cell death rate was observed in GBM control

cultured cells. Moreover, the non-GBM treatment cultured cells has

appeared to be protected from a baseline increase in cell death rate

observed in the untreated non-GBM control cultured cells (Figure 3).

e increase in cell death rate as observed in the untreated non-GBM

control cultured cells was consistent while the cancer cell viability was

Pre-treatment values were subtracted from post-treatment values

to evaluate the relative change in cell death rate aer treatment time (T

= 0) and referred as delta (δ). e δ values (as measured from before

to aer treatment per initial cell) due to bioeld treatment relative

to controls for GBM cells and non-GBM cells are shown in Figure

4. e advantage of bioeld treatment is that they are inexpensive as

compared to other types of cancer therapies. According to a recent

report regarding bioeld treatment later on, it was approved by the

German equivalent of the FDA. It was emphasized that cancer patients

have experienced healing with bioeld treatment. Nowadays, many

scientists and cutting edge practitioners believed that the secrets

of regeneration and healing lie not only on costly medical drugs or

expensive medical treatments, but also in the body's own Quantum

Energy Bioeld (QEB) [30].

Current cancer therapies have certain limitations which include

serious side eects like enormous toxicity, altered immune system and

high treatment cost. Many ancient bioeld therapies for cancer are

practiced around the world, among then Qigong therapy for cancer is

Control Treatment Control Treatment

Figure 3: Comparison of cell death rate before and after bioeld treatment.

Change in cell death rate (δ)

Figure 4: The change in cell death rate (δ) of both glioblastoma brain tumor

cells and normal brain glial cells after treatment time (T = 0) due to bioeld

treatment relative to controls.

Figure 5: Percent change of cell death after 20 hours treatment period with

# data[].attributes.attachment_link.title
The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy - PdfSR.com

# data[].attributes.attachment_link.description
Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz.

# data[].attributes.headline
	
	
	
	
	Effect of Biofield Treatment on Human Brain Tumor Cells
	

# data[].attributes.subject
	
	
	
	
	Effect of Biofield Treatment on Human Brain Tumor Cells
	

# data[].attributes.body
The study to check the potential impact of Biofield treatment on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.

# data[].attributes.attachment_link.title
"The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.Methods: The human brain tumor, GBM cultured cells were divided into two groups viz.

# data[].attributes.headline
	
	
	
	
	View Publication on Human Brain Tumor Cells | Mahendra Trivedi
	

# data[].attributes.subject
	
	
	
	
	View Publication on Human Brain Tumor Cells | Mahendra Trivedi
	

# data[].attributes.body
.
View Mahendra Kumar Trivedi biofield treatment potential impact on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.

# data[].attributes.headline
	
	
	
	
	Biofield Treatment Impact on Human Brain Tumor Cells
	

# data[].attributes.subject
	
	
	
	
	Biofield Treatment Impact on Human Brain Tumor Cells
	

# data[].attributes.body
Read study of Mahendra Kumar Trivedi on human GBM and non-GBM brain cells using two time-lapse video microscopy technique after impact of biofield treatment.

# data[].attributes.attachment_link.title
The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy

# data[].attributes.attachment_link.description
Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.

# data[].attributes.headline
	
	
	
	
	An Impact of Biofield Treatment on Glioblastoma Brain Cells
	

# data[].attributes.subject
	
	
	
	
	An Impact of Biofield Treatment on Glioblastoma Brain Cells
	

# data[].attributes.body
Glioblastoma is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells.

# data[].attributes.headline
	
	
	
	
	 Open Access Journal of Integrative Oncology | Omicsgroup
	

# data[].attributes.subject
	
	
	
	
	 Open Access Journal of Integrative Oncology | Omicsgroup
	

# data[].attributes.body
The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique.

# data[].attributes.attachment_link.readable_text
Glial cell; Glioblastoma; Brain tumor; Biofield treatment; Time-lapse video microscopy

Brain tumors are typically very heterogeneous, aggressive neoplasms at the cellular level which affects both children and adults [ 1]. Statistics based on cancer research institute explore that 1 in 161 individuals born today with brain cancer at different point of life. In the U.S., 22,850 men and women are diagnosed with brain cancer every year, and 15,320 deaths are caused by this disease [ 2]. As per World health organization classification, glioblastoma (GBM) is also known as grade IV astrocytoma [ 3]. GBM is one of the most malignant form of human brain tumor. The mean survival time for GBM patients is approximately 12 months [ 4]. Time-lapse video microscopy is a technique that aids to assess cellular behavior in real time. This technique is used to collect a two dimensional image data at different time intervals. Then, these data are converted to make a movie. This unique technique is used in various fields of cancer and stem cell biology for assessment of cell translation, division, and death [ 5, 6]. Presently, several strategies are available for the treatment of GBM such as surgical techniques, radiotherapy, chemotherapy and electrochemotherapy [ 7]. Apart from commonly used chemotherapeutic agents (such as nitrosoureas derivatives, platinum based drugs, and taxol) and gene therapy, as a novel therapeutic modality frequently being used in cancer [ 8]. However, chemotherapy suffers several major drawbacks such as all tumors are not responsive to chemotherapeutic agents to some extent, incidence of bone marrow cells damage, amenorrhea, alopecia, sexual dysfunction and adversely affect the quality of life of patients [ 9].

Malignant brain tumors like GBM are very difficult to treat now a days. The difficulties to treat against GBM are due to lack of proper preventive strategies, and unavailability of practical method for screening [ 10]. Therefore, new, more effective and better tolerated anti-tumor drugs or some alternative treatment strategy are needed. Based on the above lacunas an alternative way which may be useful to kill or eradicates the proliferative tumorous cells and simultaneously defense the normal brain glial cells. Biofield treatment is an alternative approach which may be useful to improve these unfilled spaces associated with cancer patients. The human biofield is the energetic matrix that surrounds the human [ 11]. It directly links with the cellular activity that allows the DNA to communicate faster than light and maintain intelligence in the organisms [ 12]. According to universal principles of Maxwell's equations and principle of reciprocity defines electromagnetic connections related to human biofield [ 13]. Thus, a human has ability to harness the energy from environment/Universe and can transmit into any object (living or non-living) around the Globe. The object(s) always receive the energy and responded into useful way that is called biofield energy. This process is known as biofield treatment. The biofield can be monitored using electromyography, electrocardiography and electroencephalogram [ 14]. Mr. Mahendra Trivedi's unique biofield treatment (The Trivedi Effect) has been well known for altered characteristics features of microbes [ 15- 17], improved the overall productivity of crops [ 18, 19], and also transform the structural, physical and chemical properties of materials [ 20- 23]. Exposure to biofield energy caused an increase in medicinal property, growth, and anatomical characteristics of ashwagandha [ 24].

On the basis of above facts and literature, present study was undertaken to evaluate the impact of Mr. Trivedi's biofield treatment on human GBM brain tumor cells.

Human U87 glioblastoma cells were received from California pacific medical center research institute, bioscience laboratory, San Francisco, CA. The cultures were prepared and maintained as per the protocols.

Study design and biofield treatment strategy

The GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Besides this, normal brain cultured cells were also divided into two groups such as non-GBM control and non-GBM treatment. Prior to experiment, each cell culture plate was prepared with six replicates (n=6) of both GBM and non-GBM cells. Each cell culture plate was grown side-by-side in sub-compartments of one culture plate, so that both types of cells were exposed to treatment and without treatment simultaneously. One set of culture plates (GBM and non-GBM treatment) were handed over to Mr. Trivedi for biofield treatment under laboratory condition. Mr. Trivedi provided the treatment through his energy transmission process to the culture plates in front of the time-lapse microscope to deliver biofield treatment from outside a plexiglass environmental chamber attached to the microscope without touching them. Another set was considered as control experiment in which no treatment was delivered. After treatment, all the culture plates were analyzed for assessment of biofield treatment potential. In brief, during the experiment involving biofield treatment, Mr. Trivedi was escorted to the laboratory and seated a short distance (approximately 20 cm) from the time-lapse microscope (Figure 1). During the control experiment, the cells were handled identically but Mr. Trivedi did not visit the laboratory. A laboratory technician placed the cell culture plates inside of an environmental chamber for examination by time-lapse microscopy without knowledge of the eventual treatment condition for the samples. The following day, laboratory technician has converted the acquired images into Quicktime movies and transferred the movies to a database using blinding codes for analysis.

Two time-lapse video microscopy technique

Two time-lapse video microscopy experiments were followed in human brain tumor GBM cells and normal brain cells for 20 hours time cycle. Microscopic images were acquired in every 5 minutes interval, from 10 hours prior and 10 hours following the treatment time i.e. 20 hours for each experiment, and compiled into time lapse videos. Figure 2 depicts the time schedule for two time-lapse video microscopy of cell cultures [ 25].

Criteria for analysis of cell count

For each microscopic field, the total number of cells were counted and each cell was tracked over the 20 hours period. Cells that entered the microscopic field after the initial frame were neither included, nor identified as dead at the start of the video. Cell death, divisions and emigrations (cells migrating out of the microscopic field) were counted for varying numbers of cells and count was made every single hour. After that, all the data were collected and entered into excel spreadsheets, the blinding codes were revealed and the results were sorted according to experimental group for analysis.

Results and Discussion

Since, brain tumors especially GBM are the most critical medical problem affecting major part of population, physician suggests chemoprevention along with radiation strategies to reduce the incidence and mortality rate. However, there are scientific evidence and large number of in vitro studies already reported for biofield treatment and its effect in cancer cells [ 26, 27]. Previous studies indicated that biofield energy showed an inhibitory effect on metastasis and immune responses in cancer cell models [ 11, 26, 28].

An exponential growth model was applied to the study data derived through time-lapse video microscopic technique which aims to take into account potential influence on cell death rates due to change the number of cells in each microscopic field throughout the observation period. Death counts during 10 hours period of observation were converted to rates per cell by the following formula:

Death rate = ln(N(t-1)) - ln(N(t-1) - d(t))

Where, N(t-1) is the number of cells at the start of the time period and d(t) is the number of deaths during the time period. These estimates are based on an assumption that counts are dependent on the number of cells present [ 6].

The potential impact of biofield treatment on GBM brain culture cells was assessed by comparing cell death rates before and after treatment. In non-GBM (control) group, values for cell division were not significantly analyzed due to very low cell divisions rate, as the majority of the cell counts being zero throughout the 20 hours observation period. This lack of cell division under control conditions for the normal brain cells indicated that the culture conditions were not sufficient to keep the normal cells viable throughout the extended time in culture. This is often may be the case with cultures of normal cells, whereas cancerous cells typically survive in culture more readily.

Comparing the total number of cell deaths over the 10 hours prior and 10 hours following the treatment time (T = 0) for the four groups of cells and dividing these values by the initial cell counts for each group gives a rough estimation of the potential effect of treatment. Analysis using the exponential growth model indicates that the cell death rate in GBM treatment cultured cells increased after biofield treatment however, no change in cell death rate was observed in GBM control cultured cells. Moreover, the non-GBM treatment cultured cells has appeared to be protected from a baseline increase in cell death rate observed in the untreated non-GBM control cultured cells (Figure 3). The increase in cell death rate as observed in the untreated non-GBM control cultured cells was consistent while the cancer cell viability was more robust [ 29].

Pre-treatment values were subtracted from post-treatment values to evaluate the relative change in cell death rate after treatment time (T = 0) and referred as delta (δ). The δ values (as measured from before to after treatment per initial cell) due to biofield treatment relative to controls for GBM cells and non-GBM cells are shown in Figure 4. The advantage of biofield treatment is that they are inexpensive as compared to other types of cancer therapies. According to a recent report regarding biofield treatment later on, it was approved by the German equivalent of the FDA. It was emphasized that cancer patients have experienced healing with biofield treatment. Nowadays, many scientists and cutting edge practitioners believed that the secrets of regeneration and healing lie not only on costly medical drugs or expensive medical treatments, but also in the body's own Quantum Energy Biofield (QEB) [ 30].

Current cancer therapies have certain limitations which include serious side effects like enormous toxicity, altered immune system and high treatment cost. Many ancient biofield therapies for cancer are practiced around the world, among then Qigong therapy for cancer is very popular and scientifically studied [ 31, 32]. Therefore, Mr. Trivedi's biofield treatment could be an effective (with no existing side effects), cost effective (no drugs were used) and alternate approach for cancer chemotherapy. Research on biofield treatment and its impact on cancer growth has been continuously studied by researchers, which might works as alternate and complementary medicine to prolong the life of cancerous patients along with quality life.

Overall, the study data indicates that biofield treatment has significantly increased (41%) the death rate of GBM brain tumor cells as compared to untreated GBM cells. Besides this, non-GBM treatment cultured cells showed a significant reduction of cell death rate (64%) as compared to normal brain cells (Figure 5). Based on the obtained results, it is expected that biofield treatment could be novel, cost effective and an alternative advanced strategy towards brain tumor patients.

In conclusion, the study results suggest that the biofield treatment has significantly increased (41%) the cell death rate of GBM treatment brain tumor cells, as compared to GBM control cells. In addition, biofield treatment also showed a significant reduction of cell death rate (64%) in non-GBM treated culture cells i.e. protective effect as compared to non-GBM brain cells. Based on the results, the inexpensive biofield treatment approach could be used in glioblastoma brain tumor patients in near future to improve the quality of life.

Trivedi science, Trivedi testimonials and Trivedi master wellness gratefully acknowledged to the whole team of the bioscience laboratories, 665, Third Street, San Francisco, CA for their support.

1. Dirks PB (2010) Brain tumor stem cells: the cancer stem cell hypothesis writ large. MolOncol 4: 420-430.

2. http://www.cancerresearch.org/cancer-immunotherapy/impacting-all-cancers/brain-cancer#sthash.PGiM1Pzh.dpuf

3. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, et al. (2007) The 2007 WHO classification of tumours of the central nervous system. ActaNeuropathol 114: 97-109.

4. Hide T, Kuratsu J (2009) [Progress in the study of brain tumor stem cells as treatment targets]. Brain Nerve 61: 781-789.

5. Patel S, Pine S, Rameshwar P (2013) Time-Lapse video microscopy for assessment of self-renewal and division kinetics. Protocol Exchange.

6. Taft R, Moore D, Yount G (2005) Time-lapse analysis of potential cellular responsiveness to Johrei, a Japanese healing technique. BMC Complement Altern Med 5: 2.

7. Sersa G, Cemazar M, Rudolf Z (2003) Electrochemotherapy: Advantages and drawbacks in treatment of cancer patients. Cancer Therapy 1: 133-142.

8. Castro MG, Cowen R, Williamson IK, David A, Jimenez-Dalmaroni MJ, et al. (2003) Current and future strategies for the treatment of malignant brain tumors. PharmacolTher 98: 71-108.

9. Hall E, Cameron D, Waters R, Barrett-Lee P, Ellis P, et al. (2014) Comparison of patient reported quality of life and impact of treatment side effects experienced with a taxane-containing regimen and standard anthracycline based chemotherapy for early breast cancer: 6 year results from the UK TACT trial (CRUK/01/001). Eur J Cancer 50: 2375-2389.

10. Wen PY, Kesari S (2008) Malignantgliomas in adults. N Engl J Med 359: 492-507.

11. Running A (2015) Decreased Cortisol and Pain in Breast Cancer: Biofield Therapy Potential. Evid Based Complement Alternat Med 2015: 870640.

12. McTaggart L (2003) The Field. New York, Harper Perennial.

13. Maxwell J (1865) A dynamical theory of the electromagnetic field. Phil Trans R Soc (Lond.) 155: 459-512.

14. Movaffaghi Z, Farsi M (2009) Biofield therapies: biophysical basis and biological regulations? Complement TherClinPract 15: 35-37.

15. Trivedi MK, Patil S (2008) Impact of an external energy on Staphylococcus epidermis [ATCC-13518] in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 4: 230-235.

16. Trivedi MK, Patil S (2008) Impact of an external energy on Yersinia enterocolitica [ATCC-23715] in relation to antibiotic susceptibility and biochemical reactions: an experimental study. Internet J Alternat Med 6.

17. Trivedi M, Bhardwaj Y, Patil S, Shettigar H Bulbule A (2009) Impact of an external energy on Enterococcus faecalis [ATCC-51299] in relation to antibiotic susceptibility and biochemical reactions - An experimental study. J Accord Integr Med 5: 119-130

18. Shinde V, Sances F, Patil S, Spence A (2012) Impact of biofield treatment on growth and yield of lettuce and tomato. Aust J Basic andApplSci 6: 100-105.

19. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J AgricSci 35: 22-29.

20. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of silicon, tin and lead powders. J Material SciEng 2: 125.

21. Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of vanadium pentoxide powder. J Material SciEng S11: 001.

22. Trivedi MK, Patil S, Tallapragada RM (2014) Atomic, crystalline and powder characteristics of treated zirconia and silica powders. J Material SciEng 3: 144.

23. Trivedi MK, Patil S, Tallapragada RM (2015) Effect of biofield treatment on the physical and thermal characteristics of aluminium powders. IndEng Manage 4: 151.

24. Altekar N, Nayak G (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.

25. Muzzey D, van Oudenaarden A (2009) Quantitative time-lapse fluorescence microscopy in single cells. Annu Rev Cell DevBiol 25: 301-327.

26. Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, et al. (2013) Evaluation of biofield treatment dose and distance in a model of cancer cell death. J Altern Complement Med 19: 124-127.

27. Chen KW (2008) Inhibitory effects of bio-energy therapies on cancer growth. World Sci Tech Modern Trad Chin Med 10: 144-152.

28. Gronowicz GA, Jhaveri A, Clarke LW, Aronow MS, Smith TH (2008) Therapeutic touch stimulates the proliferation of human cells in culture. J Altern Complement Med 14: 233-239.

29. McAllister SD, Chan C, Taft RJ, Luu T, Abood ME, et al. (2005) Cannabinoids selectively inhibit proliferation and induce death of cultured human glioblastomamultiforme cells. J Neurooncol 74: 31-40.

30. Marshall RJ The best kept secret in nutrition: The body's biofield communication system. Premier research labs. Austin, USA.

31. Shen MX (1997) Qigong is an effective way to defeat cancer. China Qigong Science 5: 8-10.

32. Chen K, Yeung R (2002) Exploratory studies of Qigong therapy for cancer in China. Integr Cancer Ther 1: 345-370.

# data[].attributes.attachment_link.title
The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy

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Glial cell ; Glioblastoma; Brain tumor; Biofield treatment; Time-lapse video microscopy Brain tumors are typically very heterogeneous, aggressive neoplasms at the cellular level which affects both children and adults [ 1]. Statistics based on cancer research institute explore that 1 in 161 individuals born today with brain cancer at different point of life.

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	Biofield | Impact on Spectral Properties of Disulfiram & Nicotinic Acid
	

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	Biofield | Impact on Spectral Properties of Disulfiram & Nicotinic Acid
	

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Biofield treatment on disulfiram and nicotinic acid to show the change in their chemical stability, force constant, bond strength & dipole moments. Read the clinical study on the impact of Biofield treatment on spectral properties of disulfiram and nicotinic acid.

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Khemraj Bairwa | Academic Room

# data[].attributes.attachment_link.description
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid.

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	Disulfiram & Nicotinic Acid Spectral Properties after Biofield Treatment
	

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	Disulfiram & Nicotinic Acid Spectral Properties after Biofield Treatment
	

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Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. Read this study of Mr. Trivedi to know its impact on spectral properties of Disulfiram & Nicotinic Acid.

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Analytical & Bioanalytical

Trivedi et al.,J Anal Bioanal Tech 2015, 6:5

http://dx.doi.org/10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

Keywords: Disulram; Nicotinic acid; Bioeld treatment; Fourier

transform infrared spectroscopy; Ultraviolet spectroscopy

Disulram [bis(diethylthiocarbamoyl)disulphide] is an antabuse

drug, being used clinically as an aid to the treatment of chronic

alcoholism. It is the rst drug approved by US Food and Drug

Administration to treat the alcohol addiction [1]. Alcohol (ethanol)

transforms into acetaldehyde by alcohol dehydrogenase enzyme, which

further oxidized to acetic acid by acetaldehyde dehydrogenase (ADH)

enzyme [2]. Disulram inhibits the ADH enzyme. As a result, the blood

concentration of acetaldehyde increases and causes an unpleasant

eect, thus increase the patient's motivation to remain abstinent [3]. In

addition to this, disulram is reported for protozoacidal eect in vitro

study [4,5]. Recently, disulram has shown the reactivity to latent HIV-

1 expression in a primary cell model of virus latency and presently it is

assessed in a clinical trial for its potential to diminish the latent HIV-1

reservoir in patients combination with antiretroviral therapy [6].

Nicotinic acid or niacin is one of the B-complex vitamins (Vitamin

B3 ) that has cholesterol lowering activity. Recent studies showed that

therapeutic doses of nicotinic acid induce a profound alteration in

plasma concentration of several lipids and lipoproteins, resulting in a

greater ability to increase high-density lipoprotein (HDL) cholesterol

[7]. Nicotinic acid favorably aects apolipoprotein (apo), very-low-

density lipoprotein (VLDL), low-density lipoprotein (LDL) and HDL

e exact mechanism of nicotinic acid activity is unknown.

However, new ndings indicate that nicotinic acid inhibits directly

and non-competitively to the triglycerides synthesis enzyme i.e.,

hepatocyte diacylglycerol acyltransferase-2, which causes acceleration

of intracellular hepatic apo B degradation and thus decrease secretion

of VLDL and LDL [9]. Several evidence suggest that nicotinic acid

administered either alone or in combination with other cholesterol-

lowering medicines can reduce the risk of cardiovascular and

atherosclerosis diseases. e clinical uses of nicotinic acid are

somewhat limited due to some harmless but unpleasant side eects

like cutaneous ushing phenomenon, nausea, vomiting and headache

[10]. e chemical and physical stability of pharmaceutical drugs or

products are most desired attributes of quality that potentially aect the

ecacy, safety and shelf life of drugs [11]. Hence, it is essential to nd

out an alternate approach, which could enhance the stability of drugs

by altering the structural and bonding properties of these compounds.

Contemporarily, bioeld treatment is reported to alter the spectral

properties of various pharmaceutical drugs like paracetamol, piroxicam,

metronidazole, and tinidazole; likewise physical, and structural

properties of various metals i.e., tin, lead etc. [12-14]. e conversion of

mass into energy is well known in literature for hundreds of years that

was further explained by Hasenohrl and Einstein [15,16]. According to

Maxwell JC, every dynamic process in the human body had an electrical

signicance, which generates magnetic eld in the human body [17].

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd, Bhopal-462026,

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Bairwa K, et al. (2015)

Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Disulram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex

vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of bioeld

treatment on spectral properties of disulram and nicotinic acid. The study was performed in two groups i.e., control

and treatment of each drug. The treatment groups were received Mr. Trivedi's bioeld treatment. Subsequently,

spectral properties of control and treated groups of both drugs were studied using Fourier transform infrared (FT-IR)

and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of bioeld treated disulram showed the

shifting in wavenumber of C-H stretching from 1496 to 1506 cm-1 and C-N stretching from 1062 to 1056 cm-1. The

intensity of S-S dihedral bending peaks (665 and 553 cm-1 ) was also increased in bioeld treated disulram sample,

as compared to control. FT-IR spectra of bioeld treated nicotinic acid showed the shifting in wavenumber of C-H

stretching from 3071 to 3081 cm-1 and 2808 to 2818 cm-1. Likewise, C=C stretching peak was shifted to higher

frequency region from 1696 cm-1 to 1703 cm-1 and C-O (COO- ) stretching peak was shifted to lower frequency region

from 1186 to 1180 cm-1 in treated nicotinic acid.

UV spectrum of control and bioeld treated disulram showed similar pattern of UV spectra. Whereas, the UV

spectrum of bioeld treated nicotinic acid exhibited the shifting of absorption maxima (λmax ) with respect of control

i.e., from 268.4 to 262.0 nm, 262.5 to 256.4, 257.5 to 245.6, and 212.0 to 222.4 nm.

Over all, the FT-IR and UV spectroscopy results suggest an impact of bioeld treatment on the force constant,

bond strength, and dipole moments of treated drugs such as disulram and nicotinic acid that could led to change in

their chemical stability as compared to control.

Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Khemraj Bairwa2 and Snehasis Jana2 *

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disulram and Nicotinic Acid after

Bioeld Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

is electromagnetic eld of the human body is known as bioeld and

energy associated with this eld is known as bioeld energy [18,19].

Mr. Trivedi has the ability to harness the energy from environment or

universe and can transmit into any living or nonliving object around

this Globe. e object(s) always receive the energy and responding into

useful way, this process is known as bioeld treatment.

Mr. Mahendra Kumar Trivedi's bioeld treatment (e Trivedi

Eect®) has considerably changed the physicochemical, thermal and

structural properties of metals and ceramics [14,20,21]. Growth and

anatomical characteristics of some plants were also increased aer

bioeld treatment [22,23]. Further, bioeld treatment has showed

the signicant eect in the eld of agriculture science [24,25] and

Considering the impact of bioeld treatment on physical and

structural property of metals and ceramics, the present study was

aimed to evaluate the impact of bioeld treatment on spectral

properties of disulram and nicotinic acid. e eects were analyzed

using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-

Vis) spectroscopic techniques.

e disulram and nicotinic acid (Figure 1) samples were procured

from Sigma-Aldrich, MA, USA; and each drug was divided into two

parts i.e., control and treatment. e control samples were remained

as untreated, and treatment samples were handed over in sealed pack

to Mr. Trivedi for bioeld treatment under laboratory condition.

Mr. Trivedi provided this treatment through his energy transmission

process to the treated groups without touching the sample [12,13].

e control and treated samples of disulram and nicotinic acid were

evaluated using FT-IR and UV-Vis spectroscopy.

FT-IR spectroscopic characterization

FT-IR spectra were recorded on Shimadzu's Fourier transform

infrared spectrometer (Japan) with frequency range of 4000-500 cm-1 .

e FT-IR spectroscopic analysis of both control and treated samples

of disulram and nicotinic acid were carried out to evaluate the impact

of bioeld treatment at atomic level like force constant and bond

UV-Vis spectroscopic analysis

UV spectra of disulram and nicotinic acid were recorded on

Shimadzu UV-2400 PC series spectrophotometer with 1 cm quartz

cell and a slit width of 2.0 nm. e analysis was carried out using

wavelength in the range of 200-400 nm. e analysis was performed

to determine the eect of bioeld treatment on structural properties of

FT-IR spectroscopic analysis

Vibrational spectral assignment was performed on the recorded

FT-IR spectra (Figure 2) based on theoretically predicted wavenumber

and presented in Table 1. e FT-IR spectrum of control disulram

sample (Figure 2a) showed the characteristic vibrational peak at 2975

peak observed at 1496 cm-1 was attributed to C-H symmetrical

deformation vibrations. e absorption peaks appeared at 1351-1457

cm-1 was assigned to CH2 -CH3 deformations. e vibrational peaks

at 1273 cm-1 and 1151-1195 cm-1 were assigned to C=S stretching and

C-C skeletal vibration, respectively. Further, IR peaks observed at 967-

1062 cm-1 and 818-914 cm-1 were attributed to C-N stretching and C-S

stretching, respectively. e vibrational peaks appeared at 554-666

cm-1 was assigned to S-S dihedral bending. e FT-IR data of control

disulram was well supported by the literature data [29].

e FT-IR spectrum of bioeld treated disulram (Figure 2b)

showed the vibrational peak at 2975 cm-1 ,which was assigned toCH3

stretching. Vibrational peak appeared at 1506 cm-1 was assigned to

C-H symmetrical deformation vibrations. Likely, the IR peaks at 1350-

1457 cm-1 were attributed to CH2 -CH3 deformations. e vibrational

peaks appeared at 1273 cm-1 and 1151-1195 cm-1 were assigned to

C=S stretching and C-C skeletal vibration, respectively. e IR peaks

observed at 967-1056 cm-1 and 817-914 cm-1 were attributed to C-N

stretching and C-S stretching, respectively. e vibrational peaks at

553-665 cm-1 were assigned to S-S dihedral bending.

Altogether, the FT-IR data of bioeld treated disulram (Figure 2b)

showed the shiing in frequency of some bonds with respect to control

spectra like C-H symmetrical deformation vibrations frequency was

shied from 1496 (control) to 1506 (treated) cm-1. e frequency (ν)

of vibrational peak depends on two factors i.e., force constant (k) and

Figure 1: Chemical structure of (a) Disulram and (b) Nicotinic acid.

Figure 2: FT-IR spectra of Disulram (a) control and (b) treated.

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disulram and Nicotinic Acid after

Bioeld Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

reduced mass (μ), which can be explained by following equation [30]

here, c is speed of light.

If reduced mass is constant, then the frequency is directly

proportional to the force constant; therefore, increase in frequency

of any bond suggested a possible enhancement in force constant of

respective bond and vice versa [28]. Based on this it is hypothesized

that due to increase in frequency of C-H symmetrical deformation

(1496 to 1506 cm-1), the C-H bond strength in treated disulram

might also be increased with respect of control. Contrarily, the C-N

stretching vibration in treated disulram was sied to lower frequency

region as compared to control i.e., from 1062 to 1056 cm-1. is could

be referred to decrease in C-N bond strength aer bioeld treatment in

compression to control. In addition, the intensity of IR peak appeared

at 553-665 cm-1 (S-S dihedral bending) in bioeld treated sample

was found to be increased, with respect of control peaks in the same

frequency region. e intensity of vibrational peaks of particular bond

depends on the ratio of change in dipole moment (∂µ) to change in bond

distance (∂r) i.e., the intensity is proportionally change with changes in

dipole moment and inversely change with alteration in bond distance

[31]. Based on this, it is speculated that ratio of ∂µ/∂r might be altered

in S-S bonds (appeared in the frequency region of 553-665 cm-1) with

the inuence of bioeld treatment as compared to control.

e vibrational spectral assignment of nicotinic acid was performed

on the recorded FT-IR spectra (Figure 3) based on theoretically

predicted wavenumber and presented in Table 2. e vibrational peaks

appeared at 3071-2808 cm-1 was assigned to C-H stretchings. e IR

peaks observed at 1696-1710 cm-1 and 1596 cm-1 were assigned to C=O

(COO- ) asymmetrical stretching and C=C stretching, respectively.

Absorption peaks appeared at 1417, 1323, and 1301 cm-1 were

attributed to C=N symmetric stretching, C=O symmetrical stretching,

and C-N stretching, respectively. e C-O (COO-) stretching peak

was assigned to IR bend observed at 1186 cm-1. Further, C-H in plane

and out plane bending vibrations was assigned to peaks observed in

the range of 1033-1114 cm-1 and 642-812 cm-1, respectively. e FT-IR

data of control nicotinic acid was well supported by the literature data

e FT-IR spectrum of bioeld treated nicotinic acid (Figure 3)

showed the absorption bands at 2818-3081 cm-1 that were assigned

to C-H stretching. Vibrational peaks appeared at 1703-1714 cm-1

and 1594 cm-1 were assigned to C=O (COO-) asymmetric stretching

and C=C stretching, respectively. Likewise, the IR peaks observed

at 1417, 1324, and 1301 cm-1 were assigned to C=N stretching, C=O

(COO- ) symmetric stretching, and C-N stretching, respectively. e IR

stretching. Further, the C-H in plane and out plane bending vibrations

Overall, the FT-IR data of biofield treated nicotinic acid (Figure

3) showed the shifting in wavenumber of some bonds with respect

to control sample. For instance, the C-H stretching towards higher

frequency region i.e., from 3071 to 3081 cm-1 and 2808 to 2818 cm-

1. This could be due to increase in force constant of C-H bond.

Likewise, a slight upstream shifting in C=O stretching peak from

1710 to 1714 cm-1 and 1696 to 1703 cm-1 in treated nicotinic acid

also suggests an increase in force constant of C=O bond in treated

sample as compared to control. Contrarily, a slight downstream

shifting in wavenumber of treated nicotinic acid from 1186 to 1180

cm-1 C-O (COO- stretching); and from 1033 to 1037 cm-1 (=C-H

in plane bending) suggests the decrease in force constant of C-O

bond and decrease in rigidity of =C-H bond in treated sample as

14961506C-H symmetrical deformation vibrations

1151-11951151-1195C-C skeletal vibrations

967-1062967-1056C-N stretching

818-914817-914C-S stretching

554-666553-665S-S stretching

Table 1: FT-IR vibrational peaks observed in Disulram.

Figure 3: FT-IR spectra of Nicotinic acid (a) control and (b) treated.

2808-30712818-3081C-H stretching

1696-17101703-1714C=O (COO-) asymmetric stretching

11861180C-OH (Ph-OH) stretching

1033-11141037-1116=C-H in-plane bending

642-812642-812=C-H out of plane bending

Table 2: FT-IR vibrational peaks observed in Nicotinic acid.

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid.

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	Spectroscopic Characterization of Disulfiram & Nicotinic Acid - Study more
	

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	Spectroscopic Characterization of Disulfiram & Nicotinic Acid - Study more
	

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To know more about the effect of biofield treatment on the Spectroscopic Characterization of Disulfiram and Nicotinic Acid, click this research.

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"Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid.

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	Biofield Treatment Effect on Disulfiram & Nicotinic Acid - View more
	

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	Biofield Treatment Effect on Disulfiram & Nicotinic Acid - View more
	

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View the impact of Mahendra Kumar Trivedi biofield energy on spectral properties of Disulfiram & Nicotinic Acid. Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity.

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	Significant Impact of Biofield on Disulfiram & Nicotinic Acid  Spectral Properties
	

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	Significant Impact of Biofield on Disulfiram & Nicotinic Acid  Spectral Properties
	

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The study to check the significant impact of Biofield treatment on structural property of Disulfiram and Nicotinic Acid.

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	 Spectral Properties of Disulfiram & Nicotinic Acid | Academia.edu
	

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	 Spectral Properties of Disulfiram & Nicotinic Acid | Academia.edu
	

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View Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment by Mahendra Kumar Trivedi on Academia.edu

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Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment

# data[].attributes.attachment_link.description
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral

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	Disulfiram & Nicotinic Acid - An aid in chronic alcoholism
	

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	Disulfiram & Nicotinic Acid - An aid in chronic alcoholism
	

# data[].attributes.body
See the impact of biofield energy by Mahendra Kumar Trivedi on spectral properties of Disulfiram & Nicotinic Acid. Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity.

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	Mahendra Trivedi – Healing with Human Aura Field Energy 
	

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	Mahendra Trivedi – Healing with Human Aura Field Energy 
	

# data[].attributes.body
Mahendra Trivedi has an ability to transmit a very powerful human aura field that has been scientifically proven to improve mental and physical wellbeing.

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Healers Mastery Program & Wine Experiment | Trivedi Testimonials

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	Mahendra Trivedi – Healing Relationships with Energy Medicine
	

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	Mahendra Trivedi – Healing Relationships with Energy Medicine
	

# data[].attributes.body
Biofield Energy Treatment has an ability to bring positive influence in people’s relationships thus enhancing and strengthening the bonds. Watch video for more details.

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Trivedi Effect on Relationships & Personal Life | Energy Transmission | Trivedi Testimonials

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	Mahendra Trivedi – How to Maximize your Senses 
	

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	Mahendra Trivedi – How to Maximize your Senses 
	

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Mahendra Trivedi has a unique ability to transmit a powerful form of human aura field energy that has been proven beneficial in healing various physical, mental and social disorders. Know how this energy can help in maximizing your senses to its highest potential.

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Debra’ Testimonial | Enhanced her Senses and the Confidence | Trivedi Testimonials

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	Mahendra Trivedi – How to Get Healthy Glowing skin
	

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	Mahendra Trivedi – How to Get Healthy Glowing skin
	

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Know how you can improve your skin texture and its health naturally overnight. Biofield blessed Dahryn Silver Gel helps healing your skin from beneath hence promoting healthy glow to your face.

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Impact of Trivedi Product | Dahryn Silver Gel's Effect | Trivedi Testimonials

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	Biofield | Analysis & Spectroscopic Studies of p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	Biofield | Analysis & Spectroscopic Studies of p-Chlorobenzonitrile
	

# data[].attributes.body
The analysis was done by dividing the p-CBN samples into two groups that served as control and treated. The treated group received Mr. Trivedi’s biofield treatment. Read more

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	Mahendra Trivedi Biofield Energy Treatment on p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	Mahendra Trivedi Biofield Energy Treatment on p-Chlorobenzonitrile
	

# data[].attributes.body
Research done by Mahendra Trivedi - The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

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Science Journal of Chemistry

Published online October 15, 2015 (http://www.sciencepublishinggroup.com/j/sjc)

doi: 10.11648/j.sjc.20150306.11

ISSN: 2330-0981 (Print); ISSN: 2330-099X (Online)

Physical, Thermal and Spectroscopic Studies of Biofield

Treated p-Chlorobenzonitrile

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana. Physical, Thermal and Spectroscopic

Studies of Biofield Treated p-chlorobenzonitrile. Science Journal of Chemistry. Vol. 3, No. 6, 2015, pp. 84-90.

doi: 10.11648/j.sjc.20150306.11

Para-chlorobenzonitrile (p-CBN) is widely used as a chemical inter mediate in the manufacturing of dyes,

medicines, and pesticides, however; sometimes it may cause runaway reaction s at high temperatures. The current study was

designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

The analysis was d one by dividing the p-CBN samples into two groups that served as control and treated. The treated group

received Mr. Trivedi's biofield treatment. Subsequently, the control and treated samples were evaluated using various

analytical techniques such as X-ra y diffraction (XRD), surface area analyser, differential scanning calorimetry (DSC),

thermogravimetric a nalysis (T GA), Fourier-tr ansform infra red (FT-IR) and UV-visible (UV-Vis) spectroscopy. The XRD

results showed an increase in the crystallite size (66.18 nm) of the treated sample as compared to the control sample (53.63

nm). The surface area analysis of the treated sample also showed 14.19% decrease in the surface area as compared to control.

Furthermore, DSC analysis results showed that the latent heat of fusion of the treated p-CBN increased considerably by 5.94%

as compared to control. However, the melting temperature of the treated sample did not show any considerable change from

the control sample. Besides, TGA/DTG studies showed that T

(the temperature at which the sample lost its maximum

weight) was increased by 5.22% along with a n increase in its onset of thermal d ecomposition temperature i.e. 9 6.80°C in the

biofield treated p-CBN as compared to the control sample (84.65°C). This indicates that the thermal stability of treated p-CBN

sample might increase as compared to the control sample. Ho wever, no change was found in the FT-IR and UV-Vis

spectroscopic character of the tr eated p-CBN as compared to the control. These findings suggest that the biofield treatment

Biofield Energy Treatment, Para-Chlorobenzonitrile, X-ray Diffraction Study, Surface Area Analyzer,

Differential Scanning Calorimetry, Thermogravimetric Analysis

Aromatic nitriles have wide applications in the production

of dyes, pesticides and pharmaceuticals. They are used as

intermediates in the synthesis of various pharmacologically

relaxants, neuroleptics, etc. [1].They are also widely used in

the synthesis of HIV-1 non-nucleoside reverse transcriptase

inhibitors and the arylation of oxazoles and poly ether amides

[2-4]. Benzonitriles are of great interest in the field of organic

benzonitriles are al so used in the synthesis of various drugs

like chlorazepate, climazolam and loflazepate, etc. [5]. Many

derivatives of benzonitrile are used in salt form as urinary

antiseptics and in vapour form for disinfecting bronchial

arylation on benzimidazoles [6].

The stability profile of any chemical compound is the most

desired quality that determines its shelf life and purity to be

used as an intermediate. The stability can be correlated to the

physical, thermal or structural and bonding properties of a

compound [7]. Currently, the stability of chemical compounds

in pharmaceutical industries can be affected due to altering

temperatures and pH conditions [8]. Moreover, many reactions

in industries were carried out at high temperatures. Hence, the

chemical compounds. The thermal stability is also considered

in the processing, long-term storage or shipping of material.

When a chemical has high stability, it is more resistant to

breaking down or decomposition. The higher stability also

helps to avoid runaway reactions [9]. However, benzonitriles

are reported to cause runaway reactions during chlorination

and cyanation reactions [10, 11]. Thus, it is important to search

for an alternate strategy that can improve the stability of

chemical compounds by altering their physical, thermal or

structural and bonding properties.

In recent years, biofield energy has been known for its

impact on various living organisms and non-living materials.

A biofield is the electromagnetic field that permeates and

surrounds living organisms, and the energy associated with

this field is kno wn as biofield energy [12, 13]. According to

existing theories, this biofield constitutes a dynamic living

communicating information to a nd throughout the body. It is

believed that any imbalance in this biofield energy leads t o

disease. The health of living organisms can be affected by

balancing this energy from the environment through a natural

popular in holistic medicine health care systems, and are

included in the National Centre for Complementary and

National Institute o f Health ( NIH). NCCAM p laces biofield

therapy (putative energy fields) as the subcategory o f energy

medicine among complementary and alternative medic ines.

[16, 17]. These healing treatments suggest their mechanism

upon modulating patient-environmental energy fields. T hus,

the human has the ability to harness the energy from the

environment or universe and can transmit it to any living

organism or non-living object. This process is termed as

biofield treatment. Mr. Trivedi is well-known to possess a

unique biofield energy tr eatment (The Trivedi Effect

has been significantly studi ed in different fields such as

microbiology research [18-20], agriculture research [21,22],

and biotechnology research [23,24]. Recently, it was reported

that biofield treatment has changed the atomic, crystalline

alteration in ph ysical, thermal and chemical p roperties were

also reported in materials like aluminium and ceramic oxide

[27, 28]. Hence, based on above results the curre nt study was

designed to determine the impact of biofield treatment on

physical, thermal and spectral properties of p-CBN.

p-chlorobenzonitrile (p-CBN) was procured from S D Fine

Chemicals Pvt. Ltd., India. The sample was divided into two

parts; one was kept as the control while other was subjected

control and treated samples were further characterizations

using XRD, surface area analyser, DSC, TGA, FT-IR and

UV-Vis spectroscopic techniques.

2.1. Biofield Treatment Modalities

evaluating the impact of biofield treatment. The first set of

the sample was considered as control, and no treatment was

given to this part. The second set of t he sample was handed

over to Mr. Trivedi for biofield energy treatment under

standard laboratory conditions. Mr. Trivedi provided the

biofield treatment through his energy transmission process,

which includes bioenergy emission for 3-5 min. without

touching the sample. After treatment, the sample was handed

over in the same condition and stored at standard conditions

as per the sta ndard experimental protocol. The optimum

experiments. The differences in parameters before and after

the treatment were noted and compared.

2.2. X-ray Diffraction (XRD) Study

XRD analysis was carried out on Phillips, Holland PW

1710 X-ray diffractometer system. The X-ray generator was

equipped with a copper anode with nickel filter operating at

35kV and 20mA. The wavelength of radiation used by the

acquired over the 2θ range o f 10°-99.99° at 0.02° interval

with a measurement time of 0.5 seconds per 2θ intervals. T he

data obtained were in the form of a chart of 2θ vs. intensity

and a detailed table containing peak intensity counts, d val ue

), and relative intensity (%).

The average size of crystallite (G) was calculated from the

Scherrer equation (1) with the method based on the width of

Where, k is the equipment constant (0.94), λ is the X-ray

wavelength (0.154 nm), B in radians is the full-width at half

of the peaks and θ the corresponding Bragg angle.

Percent change in crystallite size was calculated using the

Percent change in crystallite size = [(G

are crystallite size o f control and treated

powder samples respectively.

2.3. Surface Area Analysis

analyser, Smart SORB 90 based on Br unauer-Emmett-Teller

(BET). Percent changes in surface area were calculated using

Science Journal of Chemistry 2015; 3(6): 84-90 86

 

are the surface area of control

and treated samples, respectively.

2.4. Differential Scanning Calorimetry (DSC) Study

temperature and latent hea t of fusion (∆ H). The DSC curve s

heating rate of 10°C/min in the temperature range of 50°C to

350°C. An empty pan sealed with cover pan was used as a

reference sample. Melting temperature and latent heat of

fusion were obtained from the DSC curve.

Percent change in latent heat of fusion was calculated

using following equations (4) to observe the difference in

thermal properties of treated p-CBN sample as compared to

 !!!""

are the latent heat of fusion

of control and treated samples, respectively.

2.5. Thermogravimetric Analysis/ Derivative

Thermogravimetry (TGA/DTG)

Thermal stability of control and treated sample of p-CBN

thermogravimetric analyser (TGA/DTG). The samples were

heated from room temperature to 400ºC with a heating rate of

(temperature at which sample start losing

weight) and from DTG curve, T

sample lost its maximum weight) were recorded.

Percent change intemperature at which maximum weight

loss occur in sample was calculated usi ng following equation

which maximum weight loss occurs in c ontrol and treated

Percent change inonset peak temperature was calculated

using following equation (6):

% change in onset peak temperature, T

temperature in control and treated sample, respectively.

2.6. Fourier Transform-Infrared (FT-IR) Spectroscopic

The samples were crushed into fine powder for anal ysis.

The powdered sample was mixed in spectroscopic grade KBr

in an agate mortar and pressed into pellets with a hydraulic

press. FT-IR spectra were recorded on Shimadzu's Fourier

transform infrared spectrometer (Japan). FT-IR spectra are

generated by the ab sorption of electromagnetic radiation in

the frequency range 4000-400 cm

. The FT-IR spectroscopic

analysis of p-CBN was carried out to evaluate the impact of

biofield treatment at atomic and molecular level like bond

strength, stability, rigidity of structure, etc. [29].

2.7. UV-Vis Spectroscopic Analysis

wavelength range of 200-400 nm with 1 cm quartz cell and a

slit width of 2.0 nm. This analysis was performed to evaluate

the effect of biofield trea tment on the structural property of

p-CBN sample. With UV-Vis spectroscopy, it is possible to

investigate electron transfers between orbitals or bands of

atoms, ions and molecules existing in the gaseous, liquid and

3. Results and Discussion

X-ray diffraction analysis was conducted to study the

crystalline nature of the control and treated sample of p-

CBN. XRD diffractogram of control and treated samples of

p-CBN are shown in Figure 1. The XRD diffractogram of

control p-CBN showed intense crystalline peaks at 2θ equal

to 1 4.11°, 15.27°, 19.61°, 25.26° and 28.39°. The intense

peaks indicated the crystalline nature of p-CBN. However,

crystalline peak at 2θ equal to 14.05°, 15.27°, 1 9.55°, 25.16°,

and 28.34°. Most of the treate d sample peaks showed similar

crystallinity of treated p-CBN sample was not changed as

compared to control. Besides, the crystallite size was found

respectively. The crystallite size was increased b y 23.4% i n

previously reported that cyano group has donor-acceptor

dimensional network of molecules [30]. It is presumed that

molecules that may lead to the formation of a supra molecular

structure of p-CBN due to the interaction of C≡ N---Cl. This

formation of supramolecular structure may lead to increase in

crystallite size of p-CBN after biofield treatment. T his type

of interactio n was also reported by D. Britton in 2-

chlorobenzonitriles, which l ead to the formation of four

crystallographic non-equivalent for ms of molecules [31]. As

the intermolecular bonding leads to stability of the

compound, hence it suggests that biofield treatment might

enhance the stability of treated p-CBN.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile - PdfSR.com

# data[].attributes.attachment_link.description
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

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	Biofield Energy Treatment on p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	Biofield Energy Treatment on p-Chlorobenzonitrile
	

# data[].attributes.body
In this study, view an impact of Mahendra Kumar Trivedi Biofield Energy Treatment on physical, thermal and spectroscopic studies of p-Chlorobenzonitrile.

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thermal studies of para chloro benzonitrile

# data[].attributes.attachment_link.keywords[]
spectroscopic studies of p-chloro benzonitrile

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

# data[].attributes.headline
	
	
	
	
	Biofield | Properties & Spectroscopic Studies of p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	Biofield | Properties & Spectroscopic Studies of p-Chlorobenzonitrile
	

# data[].attributes.body
Mahendra Kumar Trivedi Biofield Energy Treatment on physical, thermal and spectroscopic studies of p-Chlorobenzonitrile. Read the complete study for more information.

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	Study Biofield Treatment Effect on p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	Study Biofield Treatment Effect on p-Chlorobenzonitrile
	

# data[].attributes.body
The study to check the physical, thermal and spectroscopic studies of Mahendra Kumar Trivedi Biofield Treated p-Chlorobenzonitrile. Study more

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		Spectral Properties of Biofield Treated p-Chlorobenzonitrile
	

# data[].attributes.subject
	
	
	
	
	
		Spectral Properties of Biofield Treated p-Chlorobenzonitrile
	

# data[].attributes.body
Present study was designed to evaluate the impact of Mahendra Kumar Trivedi biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

# data[].attributes.headline
	
	
	
	
	Physical Thermal & Spectroscopic Studies of Biofield Treated p-CBN
	

# data[].attributes.subject
	
	
	
	
	Physical Thermal & Spectroscopic Studies of Biofield Treated p-CBN
	

# data[].attributes.body
The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-Chlorobenzonitrile.

# data[].attributes.headline
	
	
	
	
	Science Journal of Chemistry | Biofield Treated p-Chlorobenzonitrile
	

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	Science Journal of Chemistry | Biofield Treated p-Chlorobenzonitrile
	

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Study an open access Journal of Chemistry on Biofield Treated p-Chlorobenzonitrile and also see the impact of biofield treatment on physical, thermal, and spectral properties of p-CBN.

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	Study Biofield Treated 3-Nitroacetophenone Significant Impact
	

# data[].attributes.subject
	
	
	
	
	Study Biofield Treated 3-Nitroacetophenone Significant Impact
	

# data[].attributes.body
The study to check the significant impact of Mr. Trivedi Biofield treatment on physical, thermal & spectral properties of 3-Nitroacetophenone.

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Science Journal of Analytical Chemistry

Published online October 15, 2015 (http://www.sciencepublishinggroup.com/j/sjac)

doi: 10.11648/j.sjac.20150306.11

ISSN: 2376-8045 (Print); ISSN: 2376-8053 (Online)

Physical, Thermal and Spectral Properties of Biofield

Treated 3-Nitroacetophenone

, Rama Mohan Tallapragada

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana.

Physical, Thermal and Spectral Properties of Biofield Treated 3-Nitroacetophenone. Science Journal of Analytical Chemistry.

Vol. 3, No. 6, 2015, pp. 71-79. doi: 10.11648/j.sjac.20150306.11

3-Nitroacetophenone (3-NAP) is an organic compound used as an intermediate for the synthesis of pharmaceutical

agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal and spectral

properties of 3-NAP. The study was performed in two groups i.e. control and treated. The control group remained as untreated,

and the treated group received Mr. Trivedi's biofield energy treatment. The control and treated 3-NAP samples were further

characterized by X-ray diffraction (XRD), differential scanning calo rimetry (DSC), thermogra vimetric analysis (TGA), laser

spectroscopy (UV-vis) analysis. The XRD analysis showed decrease in crystallite size of treated 3-NAP b y 20.2 7% as

compared to the control sample. However, the XRD peaks of treated sample showed an increase in intensity as compared to the

control. The DSC result showed a slight increase in melting temperature of treated 3-NAP (80.75ºC) with respect to the control

(79.39ºC). The latent heat of fusion o f treated 3-NAP was changed by 16.28% as compared to the control sample. The TGA

analysis showed an increase in onset temperature of treated sample (192ºC) as compared to the control sample (182ºC).

Further, the maximum thermal decomposition temperature (T

) of treated 3-NAP was increased as compared to the control.

This showed the increase in thermal stability of treated 3-NAP with respect to control. The treated 3-NAP showed an increase

in average particle size (d

) by 27.6% along with an increas e in size exhibited by 99% o f particles (d

to the control. Brunauer-Emmett-Teller (BET) analysis showed a substantial decrease in surface area by 24.6% with respect to

the control. The FT-IR analysis showed an emergence of peak at 1558 cm

in treated 3-NAP sample as compared to the

control. Nevertheless, the UV spectral analysis of treated 3-NAP showed no alterations in absorption pe aks as compared to the

control. Altogether, the result showed that biofield energy treatment has altered the physical, thermal and spectral properties o f

treated 3-NAP as compared to the control.

X-Ray Diffraction, Thermal Analysis, Laser Particle Size Analysis, Surface Area Analysis, Fourier Transform

Infrared Spectroscopy, Ultra Violet-Visible Spectroscopy

Nitroaromatic compounds are one of the important group

compounds consist of at least one -NO

attached to the aromatic ring [1]. Many pharmaceuticals are

synthesize the diverse collection of indoles that are bioacti ve

Nitroacetophenone are nitroaromatic compounds that have

trypanosomal drug candidates [3]. 2-Nitro acetophenone is

used as an intermediate for the synthesis of cinoxacin that is

72 Mahendra Kumar Trivedi et al.: Physical, Thermal and Spectral Properties of

Biofield Treated 3-Nitroacetophenone

administered for the treatment of inflammation and arthritis

pain [5]. Iradyan et al. synthesized 3-NAP derivatives and

investigated its antitumor efficiency [6]. Chalcones are an

pharmacological activity [7]. Chalcones are synthesized fro m

3-NAP as an intermediate compound, which have pro found

anti-inflammatory [8], anti-ulcerative [9], antibacterial [10],

Pharmaceutical stability is a key factor that d etermines the

therapeutic efficacy and to xicity of medications. According

to Food and Drug Administration (FDA), the drug companies

should determine a time limit to which they can assure the

full potency and safety of the medications [13]. Therefore,

compounds are more desired quality attributes that directly

affect its safety, efficacy, and shelf life [14]. Thus, exploring

properties of active pharmaceutical i ngredients will be like

Recently biofield energy treatment was utilized as novel

method for changing the physical and thermal properties of

various materials such as metals [15, 16], ceramics [17],

pharmaceutical drugs [19]. Therefore author planned to study

the influence of biofield energy treatment on the physical,

thermal and spectral properties of 3-NAP.

In United States t he National Centre for Complementary

and Alternative M edicine (NCCAM), which is a part of the

prestigious National Institute of Health (NIH), authorizes the

use of Complementary and Alternative Medicine (CAM)

therapies as an alternative in the healthcare sector. According

to an estimate about 36% of Americans regularly uses some

therapy is one of the energy medicine used worldwide to

alleviate overall human health.

Biofield energy treatment consists of practices based on

subtle energy field and generally reflect t he concept that

human being are infused with this form of energy [21].

Researchers have shown tha t a unique bioenergetic field

bioenergetic field regulates the human health and durin g

disease condition this unique field is depleted [23]. Recently

some medical technologies were used to measure this human

Therefore, it is envisaged that human beings have the

ability to harness the energy from the environment/Universe

and can transmit into any object (living or non-living) ar ound

the Globe. The object(s) will always receive the energy a nd

responding in a useful manner that is called biofield energy.

Mr. Trivedi is known to transform the characteristic s of

various living and non-living things using his unique biofield

energy. This biofield energy treatment is also known as The

characteristics of microbes [25, 26] and improved the growth

and anatomical characteristics of medicinal plants [27, 28].

physical, thermal and spectral properties of 3-NAP.

3-Nitroacetophenone (3-NAP) was procured from S D

Fine Chemicals Limited, India. The sample was divided into

two parts; one was kept as the control sample while the other

treatment and coded as treated sample. The treated sample

was in sealed pack and handed over to Mr. Trivedi for

biofield energy treatment under laboratory condition. Mr.

Trivedi gave the energy trea tment through his uniq ue energy

transmission process to the treated samples without touching

it. The control and treated samples were further characteriz ed

by various analytical techniques such as X-ray diffraction,

differential scanning calorimetry, thermogravimetric analysis,

laser particle size analyzer, surface area analyzer, Fourier

2.1. X-ray Diffraction (XRD) Study

XRD analysis of control and treated 3-NAP was evaluated

using X-ray diffractometer system, Phillips, Holland PW

1710 which consist of a copper anode with nickel filter. XRD

average crystallite size (G) was computed using formula:

Here, λ is the wavelength of radiation used, b is full width

half-maximum (FW HM) o f peaks and k is the equipment

constant (=0.94). Percentage change in average crystallite

size was calculated using following formula:

Percentage change in crystallite size = [(G

are denoted as crystallite size of control

and treated powder samples respectively.

2.2. Differential Scanning Calorimetry (DSC)

The control and treated 3-NAP samples were analyzed

10ºC/min under air atmosphere and the air was flushed at a

flow rate of 5 mL/min. The predetermined amount of sample

was kept in an aluminum pan and clo sed with a lid. A blank

change in latent heat of fusion was calculated using



are represented as the latent

heat of fusion of control and treated samples, respectively.

Science Journal of Analytical Chemistry 2015; 3(6): 71-79 73

2.3. Thermogravimetric Analysis-Differential Thermal

thermal analyzer (DTA) was used to investigate the thermal

stability of control and treated 3-NAP samples. The heating

rate was 5ºC/min and the samples were heated in the range of

room temperature to 400ºC under air atmosphere.

2.4. Particle Size Analysis

A Sympetac Helos-BF laser particle size analyzer was

samples using a measurement range of 0.1 to 875 µm. The

powder particles were calcula ted. The percentage changes in

values were computed using following formula:

2.5. Surface Area Analysis

surface area analyzer with a detection range of 0.2-1000 m

was used to evaluate the surface area of 3-NAP samples. The

samples were analyzed using a standard ASTM D 5604

method. The Percent changes in surface area were computed

$$  %

are the surface area of control

and treated samples respectively.

The FT-IR spectra were recorded on Shimadzu's Fourier

transform infrared spectrometer (Japan) with the frequency

. The analysis was accomplished to

evaluate the effect of biofield treatment at the ato mic level

like dipole moment, force constant and bond strength in

chemical structure [29]. The treated sample was divided into

two parts T1 and T2 for FT-IR analysis.

2.7. UV-Vis Spectroscopic Analysis

A Shimadzu UV-2400 PC series spectr ophotometer with 1

cm quartz cell and a slit width of 2.0 nm was used to o btain

the UV spectra of t he control and treated 3-NAP samples.

The spectroscopic analysis was carried out using wavelength

in the range of 200-400 nm and methanol was used as a

solvent. The UV spectra were analyzed to determine the

effect o f biofield treatment on the energy gap of highest

occupied molecular o rbital (HOMO) and lowest unoccupied

molecular orbital (LUMO) [2 9]. The treated sample was

divided in two parts T1 and T2 for the UV-Vis spectroscopic

Fig. 1. XRD diffractograms of control and treated 3-nitroacetophenone.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Rakesh Kumar Mishra, Snehasis Jana

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 3-Nitroacetophenone - PdfSR.com

# data[].attributes.attachment_link.description
3-Nitroacetophenone (3-NAP) is an organic compound used as an intermediate for the synthesis of pharmaceutical agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of 3-NAP. The study was performed in two groups i.e. control and treated.

# data[].attributes.headline
	
	
	
	
	An impact of Biofield Treatment on 3-Nitroacetophenone Compunds
	

# data[].attributes.subject
	
	
	
	
	An impact of Biofield Treatment on 3-Nitroacetophenone Compunds
	

# data[].attributes.body
To know the impact of Mahendra Kumar Trivedi Biofield Energy transmission on the physical, thermal and spectral properties of 3-Nitroacetophenone. Study this research.

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectral Properties of Biofield Treated 3-Nitroacetophenone" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
3-Nitroacetophenone (3-NAP) is an organic compound used as an intermediate for the synthesis of pharmaceutical agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of 3-NAP. The study was performed in two groups i.e. control and treated.

# data[].attributes.headline
	
	
	
	
	Biofield | Properties of Biofield Treated 3-Nitroacetophenone
	

# data[].attributes.subject
	
	
	
	
	Biofield | Properties of Biofield Treated 3-Nitroacetophenone
	

# data[].attributes.body
Mahendra Kumar Trivedi - Test to investigate the impact of Biofield Energy transmission on the physical, thermal and spectral properties of 3-Nitroacetophenone.

# data[].attributes.headline
	
	
	
	
	 Experimental Study on Biofield Energy treated 3-Nitroacetophenone
	

# data[].attributes.subject
	
	
	
	
	 Experimental Study on Biofield Energy treated 3-Nitroacetophenone
	

# data[].attributes.body
Experimental study of this experiment by Mahendra Kumar Trivedi, showed the physical, thermal, and spectral properties of biofield treated 3-Nitroacetophenone.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 3-Nitroacetophenone

# data[].attributes.attachment_link.description
3-Nitroacetophenone (3-NAP) is an organic compound used as an intermediate for the synthesis of pharmaceutical agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of

# data[].attributes.headline
	
	
	
	
	Physical & Thermal Properties of Biofield Treated 3-Nitroacetophenone
	

# data[].attributes.subject
	
	
	
	
	Physical & Thermal Properties of Biofield Treated 3-Nitroacetophenone
	

# data[].attributes.body
Present study aim was to evaluate the impact of biofield energy treatment on the physical, thermal & spectral properties of 3-Nitroacetophenone. View more

# data[].attributes.headline
	
	
	
	
	Spectral Properties of Biofield Treated 3-Nitroacetophenone – Read more
	

# data[].attributes.subject
	
	
	
	
	Spectral Properties of Biofield Treated 3-Nitroacetophenone – Read more
	

# data[].attributes.body
The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal & spectral properties of 3-Nitroacetophenone. Read more

# data[].attributes.headline
	
	
	
	
	Biofield Treated 3-Nitroacetophenone Compounds | Sciencepublishinggroup
	

# data[].attributes.subject
	
	
	
	
	Biofield Treated 3-Nitroacetophenone Compounds | Sciencepublishinggroup
	

# data[].attributes.body
3-Nitroacetophenone is an organic compound used as an intermediate for the synthesis of pharma ceutical agents. The aim of this study was to evaluate the impact of biofield energy treatment on the physical, thermal & spectral properties of 3-NAP.

# data[].attributes.headline
	
	
	
	
	



	
	
	
	
	



	
	
	
	
	Biofield | Antimicrobial Sensitivity Pattern  of Citrobacter braakii
	





	

# data[].attributes.subject
	
	
	
	
	



	
	
	
	
	



	
	
	
	
	Biofield | Antimicrobial Sensitivity Pattern  of Citrobacter braakii
	





	

# data[].attributes.body
Biofield treatment is an alternative approach which may be useful to improve the antimicrobial sensitivity pattern of Citrobacter braakii.

# data[].attributes.attachment_link.readable_text
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Research ArticleOpen Access

Trivedi et al., J Clin Med Genom 2015, 3:1

http://dx.doi.org/10.4172/jcmg.1000129

Research ArticleOpen Access

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

Journal of Clinical & Medical Genomics

Keywords: Citrobacter braakii; Antimicrobial susceptibility; Bioeld

treatment; Biochemical reaction; Biotype; 16S rDNA analysis; Gram-

negative bacteria; Enterobacteriaceae

Abbreviations: MDR: Multi-Drug Resistant; ATCC: American Type

Minimum Inhibitory Concentration;OTUs: Operational Taxonomic

Units;NCBI: National Center for Biotechnology Information;MEGA:

Molecular Evolutionary Genetics Analysis; PCR: Polymerase Chain

Reaction; RDP: Ribosomal Database Project; HBMEC: Human Brain

Microvascular Endothelial Cells

Citrobacter braakii (C. braakii) is a genus of Gram-negative, straight,

facultative anaerobic and motile bacilli bacterium widely distributed in

water, soil, and food in the environment. It is also commonly found in

urinary, intestinal, and respiratory tract of human and animals, belongs

to Enterobacteriaceae family. It has been associated with various

nosocomial and community acquired infections in humans [1]. Arens

et al. reported about 11 genetically distinct species within the genus

Citrobacter [2]. e main clinical manifestations have been reported

due to nosocomial infections of Citrobacter species such as bacteremias

[3], endocarditis [4], urinary tract infections [1], neonatal meningitis

[5], pneumonia [6] and brain abscess [7]. Based on literature it has

been demonstrated that 0.8% of Gram-negative infections caused by

causes nosocomial infection among all Enterobacteriaceae family

[9]. Although it has low virulence property responsible for host cell

invasion instead of it invade blood brain barrier (BBB) of human brain

microvascular endothelial cells (HBMEC) and causes meningitis.

Moreover, due to overproduction of chromosomal β-lactamase

enzyme leads to antimicrobials resistance [10]. Aminoglycosides,

uoroquinolones, carbapenems, new oral cephems and many third and

fourth-generation cephems, such as cefepime and cefpirome, are the

drugs of choice to treat C. braakii associated infections but it possess

high level of resistance against penicillin and other antibiotics[11,12].

erefore, an alternative strategy is needed to alter the antimicrobial

sensitivity prole against C. braakii strain. In recent years, bioeld

treatment was proved to be an alternative method which has impact on

various properties of living and non-living materials in a cost eective

manner. It is already demonstrated that energy can neither be created

nor be destroyed but it can be transferred through various processes such

as thermal, chemical, kinetic, nuclear, etc. [13-15]. Similarly, electrical

current exists inside the human body in the form of vibratory energy

particles like ions, protons, and electrons and they generate magnetic

eld in the human body [16,17]. Aerward, Harold Saxton Burr had

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research

Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad

Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91 755 666 0006; E-mail:

Received August 20, 2015; Accepted September 23, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, et al. (2015)

braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study

was attempted to investigate the effect of Mr. Trivedi's bioeld treatment on C. braakii in lyophilized as well as

revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized

vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further

subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed

on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes.

First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were

studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample

was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial

susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated

cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to

susceptible after bioeld treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the

treated samples as compared to the control. Biochemical reactions also showed signicant (42.42%) alteration in

the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA

(53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus)

on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species

were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and

(53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed

that the identied microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The

results suggested that bioeld treatment has a signicant impact on C. braakii in lyophilized as well as revived state.

Phenotyping and 16S rDNA Analysis after Biofield Treatment on

1Trivedi Global Inc., Eastern Avenue Suite A-969, Henderson, NV, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Chinar Fortune City, Hoshangabad Rd., Madhya Pradesh, India

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

performed the detailed studies on the correlation of electric current

with physiological process and concluded that every single process in

the human body had an electrical signicance [18]. Recently, it was

discovered that all electrical process happening in body have strong

relationship with magnetic eld as mentioned by Ampere's law, which

states that the moving charge produces magnetic elds in surrounding

space [19,20]. us, the human body emits the electromagnetic waves

in form of bio-photons, which surround the body and it is commonly

known as bioeld. erefore, the bioeld consists of electromagnetic

eld, being generated by moving electrically charged particles (ions,

cell, molecule, etc.) inside the human body. According to Rivera-Ruiz

et al., it was reported that electrocardiography has been extensively

used to measure the bioeld of human body [21]. us, human has the

ability to harness the energy from environment or universe and can

transmit into any living or nonliving object(s) around the Globe. e

objects always receive the energy and responding into useful way that

is called bioeld energy and the process is known as bioeld treatment.

Mr. Trivedi's unique bioeld treatment (e Trivedi eect®) has been

known to transform the structural, physical and thermal properties of

several metals and ceramic in materials science [22-24], improved the

overall productivity of crops [25,26], altered characteristics features of

microbes [27-29] and improved growth and anatomical characteristics

of medicinal plants [30,31].

Due to the clinical signicance of this organism and literature on

bioeld treatment, the present work was undertaken to evaluate the

impact of bioeld treatment on C. braakii in relation to antimicrobials

susceptibility and biotyping based on various biochemical characters

followed by 16S rDNA sequencing analysis.

C. braakii, American Type Culture Collection (ATCC 43162) strain

was procured from MicroBioLogics, Inc., USA and stored with proper

storage conditions until further use. All the tested antimicrobials and

biochemicals were procured from Sigma-Aldrich (MA, USA). e

antimicrobial susceptibility, biochemical reactions and biotype number

were estimated with the help of MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA, USA) using Negative Breakpoint Combo

30 (NBPC 30) panel with respect to control group (Gr.). e 16S rDNA

sequencing study was carried out using ultrapure genomic DNA prep

kit; Cat KT 83 (Bangalore Genei, India).

e impact of bioeld treatment on tested bacterium C. braakii was

Group I: ATCC strain was revived from lyophilized state and

considered as control. No treatment was given and analyzed for

antimicrobial sensitivity, biochemical reactions and biotype number as

per the standard protocol.

two parts named as Gr. IIA and Gr. IIB. Both the groups of ATCC

strain of C. braakii in lyophilized state were assigned to the Mr. Trivedi's

unique bioeld treatment (rst treatment). Gr. IIB sample was stored in

lyophilized state for 159 days at -70ºC. Gr. IIBwas further sub-divided

in two separate parts named as Gr. IIB - Study Iand Gr. IIB - Study II.

Aer 159 days, antimicrobial sensitivity, MIC, biochemical

reactions and biotyping were performed as per the standard protocol.

e stored strain was revived from -70ºC and the revived culture

was again provided to Mr. Trivedi's bioeld treatment (re-treatment)

on day 159. Aer bioeld retreatment, the sample was sub-cultured

into three separate tubes on 3 dierent days (Day 0, Day 5 and Day

10) and analysed keeping the main treated tube aside. Each sample was

analyzed aer 5 days of its sub-culturing.

Bioeld treatment strategy

e lyophilized (Gr. IIA) sample of C. braakii was subjected to Mr.

Trivedi's bioeld treatment (rst treatment) followed by retreatment

aer storing for 159 days in revived state (Gr. IIB, Study II). In details,

the treatment groups in sealed pack were handed over to Mr. Trivedi for

bioeld treatment under laboratory conditions. Mr. Trivedi provided

the treatment through his energy transmission process to the treated

groups without touching the samples. Aer rst treatment, the analysis

of Gr. IIA lyophilized sample was done on day 10 for antimicrobial

sensitivity along with minimum inhibitory concentration (MIC),

biochemical reactions with biotype number and 16S rDNA analysis as

per the standard protocol. While handing over these cultures to Mr.

Trivedi for retreatment purposes, optimum precautions were taken to

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of C. braakii was

carried out with the help of automated instrument, MicroScan Walk-

Away® using NBPC 30 panel. e panel can be stored at 2 to 25ºC for

analysis. e panel was allowed to equilibrate to room temperature

prior to rehydration. All opened panels were used on the same day.

e tests carried out on MicroScan were miniaturized of the broth

dilution susceptibility test that has been dehydrated. Briey, 0.1 mL

of the standardized suspension of C. braakii was pipetted into 25

mL of inoculum water using pluronic and inverted 8 to 10 times and

inoculated, rehydrated, and then subjected to incubation for 16 hours at

35°C. Rehydration and inoculation was performed using the RENOK®

system with inoculators-D (B1013-4). 25 mL of standardized inoculum

suspension was poured in to inoculum tray. e detailed experimental

procedure and conditions were followed as per the manufacturer's

instructions. e antimicrobial susceptibility pattern (S: Susceptible,

R: Resistant; and I: Intermediate) and MIC values were determined by

observing the lowest antimicrobial concentration showing inhibition

Biochemical reaction studies

Biochemical reactions of C. braakii were determined using

MicroScan Walk-Away®, system with NBPC 30 panel. Preparation of

NBPC 30 panel, inoculum followed by dehydration and rehydration

were performed in a similar way as mentioned in antimicrobial

susceptibility assay for analysis of biochemical reactions followed by

biotype number. e detailed experimental procedures and conditions

were followed as per the manufacturer's instructions [32].

Identication of organism by biotype number

e biotype number of C. braakii was determined on MicroScan

Walk-Away® processed panel data report with the help of biochemical

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from C. braakii cells (Gr. IIA, sample coded

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

as 4A) using genomic purication kit, according to the manufacturer

instructions. 16S rDNA gene (~ 1.5 kb) fragment was amplied with the

help of high-delity polymerase chain reaction (PCR) using universal

primers; forward primer (5'-AGAGTTTGATCCTGGCTCAG-3') and

reverse primer (3'-ACGGTCATACCTTGTTACGACTT-5'). Amplied

products were subjected to gel electrophoresis in 1.0% agarose gel,

stained with ethidium bromide and visualized under UV light in a gel

documentation unit (BioRad Laboratories, USA). e PCR amplied

fragment was puried from the agarose gel using a DNA gel extraction

kit. Sequencing of amplied product was done on commercial basis

from Bangalore Genei, India. e 16S rDNA sequences obtained were

aligned and compared with the sequences stored in GenBank database

available from National Center for Biotechnology Information (NCBI)

using the algorithm BLASTn program. Multiple sequence alignment/

phylogenetic tree were established using MEGA3.1 molecular soware

Antimicrobial susceptibility test

e results of C. braakii susceptibility pattern and MIC values of

tested antimicrobials aer bioeld treatment are presented in Table 1

and 2 respectively. e data were analyzed and compared with respect

to control (Gr. I). Antimicrobial susceptibility assay was carried out

using twenty-eight antimicrobials. Overall, the treated cells of C.

braakii showed 39.29% alteration in antimicrobial sensitivity pattern

as compared to control. e sensitivity pattern of tetracycline was

changed from resistance (R) to susceptible (S) and simultaneously

decreased MIC value by two folds (>8 to ≤ 4 µg/mL) in all the treated

groups as compared to control (Gr. I). is improvement with

respect to resistant pattern and MIC value could be due to bioeld

treatment. e eect was observed throughout the experiment. So,

it may assume that the eect of bioeld treatment was sustainable.

Moreover, the antibiogram pattern of certain antimicrobials viz.

aztreonam, cefotaxime, cefotetan, ceazidime, ceriaxone, cefuroxime,

piperacillin/tazobactam, piperacillin and ticarcillin/k-clavulanate were

changed from inducible β-lactamase (IB) to susceptible in lyophilized

treated (rst treatment) Gr. IIB, Study I on day 159 as well as in revived

treated (second treatment) Gr. IIB, Study II on day 5 as compared

to untreated sample (Gr. I). e microbe C. braakii has the ability to

produce chromosomal β-lactamases. us, the overproduction of

this enzymes lead to resistance in most of penem and cephems ring

containing antimicrobials [34].

However, aer bioeld treatment the above mentioned nine

antimicrobials were converted from IB to completely susceptible

in lyophilized treated (rst treatment) Gr. IIB, Study I on day 159 as

well as in revived treated (second treatment) Gr. IIB, Study II on day

5 as compared to control sample. However, the susceptibility pattern

of these nine antimicrobials did not show any alteration in rest of

the treated samples as compared to control. is alteration could be

due to exertion of bioeld energy to the treated samples at enzymatic

S. No.AntimicrobialGr. I (Control)Gr. IIA (Day 10)Gr. IIB (Study I; Day

Gr. IIB (Study II; Day 159)

3.Ampicillin/sulbactamI I I I I I

4.AmpicillinR R R R R R

8. CefotaximeIB IB S S IB IB

12. CeftriaxoneIBIBSSIBIB

15.Chloramphenicol S S S S S S

16. Ciprooxacin S S S S S S

18. GentamicinS S S S S S

20. Levooxacin S S S S S S

21.MeropenemS S S S S S

24.PiperacillinIB IB SSIB IB

25.TetracyclineR S S S S S

26. Ticarcillin/k-clavulanateIBIBSSIBIB

27.TobramycinS S S S S S

/sulphamethoxazole S S S S S S

R: Resistant; S: Susceptible; I: Intermediate; IB: Inducible β-lactamase; Gr.: Group

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii - PdfSR.com

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	
	Citrobacter braakii: A Urinary Pathogen | Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	Citrobacter braakii: A Urinary Pathogen | Biofield Treatment
	

# data[].attributes.body
Main objective of present study was to study the effect of Mahendra Kumar Trivedi biofield energy on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C.

# data[].attributes.attachment_link.keywords[]
16s rdna analysis of citrobacter braakii

# data[].attributes.attachment_link.title
"Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	
	Biofield & Phenotyping  Analysis on Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	Biofield & Phenotyping  Analysis on Citrobacter braakii
	

# data[].attributes.body
Biofield is the scientifically preferred term for the biologically produced electromagnetic and subtle energy field that provides regulatory and communication functions within the organism. Present study was attempted to investigate the effect of Mahendra Kumar Trivedi biofield treatment on C. braakii

# data[].attributes.headline
	
	
	
	
	Biofield | Phenotyping & Analysis of Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	Biofield | Phenotyping & Analysis of Citrobacter braakii
	

# data[].attributes.body
The present study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for

# data[].attributes.headline
	
	
	
	
	Biofield Therapy on Citrobacter braakii: A Urinary Pathogen
	

# data[].attributes.subject
	
	
	
	
	Biofield Therapy on Citrobacter braakii: A Urinary Pathogen
	

# data[].attributes.body
Present study was attempted to investigate the effect of Mahendra Kumar Trivedi biofield treatment on C. braakii in lyophilized vial of ATCC strain of C.

# data[].attributes.headline
	
	
	
	
	Biofield | Phenotyping Analysis of Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	Biofield | Phenotyping Analysis of Citrobacter braakii
	

# data[].attributes.body
Citrobacter braakii is a Gram-negative species of bacteria. The main purpose of this study was to analyze the impact of biofield treatment on Citrobacter braakii: A Urinary Pathogen.

# data[].attributes.headline
	
	
	
	
	Phenotyping & 16S rDNA Analysis of Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	Phenotyping & 16S rDNA Analysis of Citrobacter braakii
	

# data[].attributes.body
The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C.

# data[].attributes.headline
	
	
	
	
	An Effect of Biofield Treatment on Streptococcus group B
	

# data[].attributes.subject
	
	
	
	
	An Effect of Biofield Treatment on Streptococcus group B
	

# data[].attributes.body
Mahendra Kumar Trivedi investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

# data[].attributes.attachment_link.readable_text
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Volume 7(5): 269-273 (2015) - 269

ISSN: 1948-5948 JMBT, an open access journal

Research ArticleOpen Access

Trivedi et al., J Microb Biochem Technol 2015, 7:5

http://dx.doi.org/10.4172/1948-5948.1000223

Research ArticleOpen Access

Microbial & Biochemical Technology

*Corresponding author:Snehasis Jana, Trivedi Global Inc., 10624 S Eastern

Avenue Suite A-969, Henderson, NV 89052, USA, Tel: +91-755-6660006; E-mail:

Received August 12, 2015; Accepted September 01, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015)

Effect of Bioeld Energy Treatment on Streptococcus group B: A Postpartum

Pathogen. J Microb Biochem Technol 7: 269-273. doi: 10.4172/1948-5948.1000223

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial

septicemia and neonatal meningitis. The current study was attempted to investigate the effect of bioeld treatment

on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae

gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number

and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains

of S. agalactiae gr. B were selected for the study. Gr. I was considered as control. Both revived (Group; Gr. II)

and lyophilized (Gr. III) strains of S. agalactiae gr. B were subjected to Mr. Trivedi's bioeld treatment. Gr. II was

assessed on day 5 and day 10 while Gr. III on day 10 with respect to the control (Gr. I) using MicroScan Walk-Away®

system. Although bioeld treatment did not show any change with respect to susceptibility pattern. However the

minimum inhibitory concentration of S. agalactiae gr. B showed signicant (70.37%) alteration, out of twenty-seven

in lyophilized group (Gr. III) as compared to the control. Moreover, the improvement of MIC value of noroxacin was

observed by two-fold (8 to ≤4 µg/mL) in Gr. II on day 10 after bioeld energy treatment as compared to the control.

It was observed that overall 48.28% biochemical reactions, out of twenty-nine were altered in Gr. II with respect to

the control. Moreover, biotype numbers were changed in Gr. II on day 5 (777777615) and on day 10 (757677405)

as compared to the control (237147047). The results suggest that bioeld treatment has signicant impact on S.

agalactiae gr. B in revived treated cells (Gr. II) with respect to MIC values, biochemical reactions pattern and biotype

Effect of Biofield Energy Treatment on

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Harish Shettigar1, Sambhu Charan Mondal2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Madhya Pradesh, India

Minimum inhibitory concentration; Biochemical reaction; Biotype

Abbreviations: CLSI: Clinical and laboratory standards institute;

GBS: Group B Streptococci; CAMP: Christie-Atkins-Munch-Petersen;

CDC: Centers for Disease Control and Prevention; ACOG: American

College of Obstetricians and Gynecologists; AAP: American Academy

of Pediatrics; MIC: Minimum inhibitory concentration; ATCC:

American Type Culture Collection; PBPC 20: Positive Breakpoint

Group B Streptococci (GBS), or Streptococcus agalactiae group B,

are Gram-positive, non-spore forming, non-motile, β-hemolytic and

chain-forming cocci bacteria. It is mainly inhabitant in human gut ora

and female urogenital tract [1]. Pregnant women [2] and neonates [3] are

the main victim host of this organism. It can be transferred to neonates

through the birth canal and causes bacterial septicaemia and neonatal

meningitis [4]. Most of GBS, produce Christie-Atkins-Munch-Petersen

(CAMP) factor or protein B an extracellular cytolytic protein and

β-lysin from Staphyllococcus species are jointly lysed the erythrocytes

[5]. It is estimated that in US over 70,000 cases of GBS diseases

were prevented [6]. Several antibiotics such as penicillin, ampicillin,

amoxicillin, cephalosporins (all three generations), macrolides,

clindamycin and vancomycin (as alternative) have been used to treat

against GBS infections. Among above mentioned antibiotics penicillin

is the drug of choice next to cefazolin. However, it also have certain

limitations such as high minimum inhibitory concentration (MIC), a

factor associated with high level of bacteremia and more concentration

of microbes in tissue, especially in cerebrospinal uid [7,8]. erefore,

some alternative treatment strategies are needed to overcome these

lacunas against β-hemolytic strain of gr. B Streptococci. Bioeld

treatment has been known as an alternative approach that may be

useful for S. agalactiae group B infected patients.

Researchers have shown that short-lived electrical events or action

potential exist in the several type of mammalian cells such as neurons,

muscles, and endocrine cells [9]. For instance, when the cells present

in central nervous system of human body communicate with each

another by means of electrical signals that propagate along the nerve

impulses. erefore, it was hypothesized that bioeld exists around

the human body and evidence was found using electromyography,

electrocardiography and electroencephalogram [10]. us, the human

body emits the electromagnetic waves in the form of bio-photons,

which surrounds the body and it is commonly known as bioeld.

erefore, the bioeld consists of electromagnetic eld, being generated

by moving electrically charged particles (ions, cell, molecule etc.) inside

the human body. Rivera-Ruiz reported that electrocardiography has

been extensively used to measure the bioeld of human body [11].

us, human has the ability to harness the energy from environment or

Universe and can transmit into any living or nonliving object(s) around

the Globe. e objects always receive the energy and responding into

useful way that is called bioeld energy and the process is known as

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Effect of Bioeld Energy Treatment on Streptococcus group B: A

Postpartum Pathogen. J Microb Biochem Technol 7: 269-273. doi:10.4172/1948-5948.1000223

Volume 7(5): 269-273 (2015) - 270

ISSN: 1948-5948 JMBT, an open access journal

bioeld treatment that is also called as Trivedi eect®. Mr. Trivedi's

unique bioeld treatment has been known to transform the structural,

physical, and thermal properties of several metals in material science

[12-14], improved the overall productivity of crops [15,16], altered

characteristics features of microbes [17-19] and improved growth and

anatomical characteristics of various medicinal plants [20,21].

Due to the clinical signicance of this organism and literature

reports on bioeld treatment, the present work was undertaken to

evaluate the impact of bioeld treatment on S. agalactiae group B

in relation to antimicrobials susceptibility and bio typing based on

various biochemical characters.

S. agalactiae group B, American Type Culture Collection (ATCC

12386) strains were procured from MicroBioLogics, Inc., USA, in

two sets A and B. Two dierent sealed packs were stored with proper

storage conditions until further use. All the tested antimicrobials and

biochemicals were procured from Sigma-Aldrich, MA, USA. e

antimicrobial susceptibility, biochemical reactions and biotype number

were estimated with the help of MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA, USA) using Positive Breakpoint Combo 20

(PBPC 20) panel with respect to the control group.

Two ATCC samples A and B of S. agalactiae gr. Bwere grouped

(Gr.). ATCC A sample was revived and divided into two parts Gr.I

(control) and Gr.II (revived); likewise, ATCC B was labeled as Gr.III

Bioeld treatment strategy

e Gr. I remained as untreated. e treatment Gr. II and III

in sealed pack were handed over to Mr. Trivedi for bioeld energy

treatment under laboratory condition. Mr. Trivedi provided the

treatment through his energy transmission process which includes

bioenergy emission to the treated groups (Gr. II and Gr. III) without

touching the samples. Aer treatment, sample was handed over in the

same condition and stored at standard conditions as per the standard

experimental protocol. An optimum precautionary measure were taken

while evaluating the study parameters throughout the experiments.

e dierences in parameters before and aer the treatment were

noted and compared. Gr.II was assessed at two time point i.e. on day

5 and day 10, while Gr. III was assessed on day 10 for antimicrobial

susceptibility, MIC, biochemical reactions pattern, and biotyping.

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of S. agalactiae gr. B

was carried out with the help of automated instrument, MicroScan

Walk-Away® system using PBPC 20 panel as per the clinical and

laboratory standards institute (CLSI) guidelines. e test was carried

out on MicroScan, which was miniaturized of the broth dilution

susceptibility test that has been dehydrated. Briey, the standardized

suspension of S. agalactiae gr. Bwas inoculated, rehydrated, and then

subjected to incubation for 16 hours at 35°C. e detailed experimental

procedures and conditions were followed as per the manufacturer's

instructions. e antimicrobial susceptibility pattern and MIC were

determined by observing the lowest antimicrobial concentration

showing growth inhibition [22].

Biochemical reaction studies

Biochemical reactions of S. agalactiae gr. Bwere determined using

MicroScan Walk-Away®, system with PBPC 20 panel [22]. Biochemicals

used in the study were arabinose, arginine, bacillosamine, bile

esculin, β-lactamases, crystal violet, hemolysin, indoxyl phosphatase,

inulin, acidication lactose, mannitol, mannose, micrococcus screen,

sodium chloride, nitrate, novobiocin, optochin, p-nitro phenyl β-D-

glucuronide, p -nitro phenyl β-D-galactopyranoside, phosphatase,

pyruvate, pyrolidonyl arylamidase, ranose, rambose, sorbitol,

thymidine free growth, acidication trehalose, urea, and Voges-

Identication of organism by biotype number

e biotype number of S. agalactiae gr. Bwas determined on

MicroScan Walk-Away® processed panel data report with the help of

biochemical reactions data [22].

Antimicrobial susceptibility test

e outcomes of MIC values of tested antimicrobials against S.

agalactiae gr. B aer bioeld treatment are summarized in Table 1. e

data were analyzed using automated system and compared with respect

to the control. In this experiment, twelve antibiotics were used to

investigate the susceptibility pattern on GBS viz. ampicillin, cefepime,

cefotaxime, ceriaxone, chloramphenicol, clindamycin, levooxacin,

linezolid, ooxacin, penicillin, tetracycline, and vancomycin. Out

of these, ve antibiotics showed susceptible in the control sample. It

has been evidenced from literatures that penicillin and its derivatives

are the choice of drugs against gr. B streptococcal infection [8,9].

However, bioeld treatment did not show any alteration with respect

to susceptibility pattern in all the treated groups aer bioeld treatment

(data not shown). Besides sensitivity assay, the MIC values of tested

antimicrobials were signicantly (70.37%) altered out of twenty seven

as compared to the control. e MIC value of noroxacin was reduced

by two-fold to ≤ 4 µg/mL aer bioeld treatment in Gr. II on day 10

as compared to the control (8 µg/mL). e MIC values of cefazolin,

cephalothin, and chloramphenicol were changed from ≤ 8 to >16 µg/

mL in Gr. II on day 5 and 10 as compared to the control. e MIC values

of rifampin and synercid were changed from ≤ 1 to >2 µg/mL in Gr. II

on day 5 and 10 as compared to the control. Alteration of MIC values of

cefotaxime and ceriaxone were changed from ≤8 to 32 µg/mL in Gr.

II on day 5 and 10 as compared to the control. Moreover, MIC values

of amoxicillin/k-clavulanate and trimethoprim/sulfamethoxazole

were slightly changed from ≤4/2 to >4/2 µg/mL (on day 5 and 10) and

≤2/38 to >2/38 µg/mL (on day 5) respectively in Gr. II aer bioeld

treatment as compared to the control. Antimicrobial linezolid showed

an alteration of MIC value in Gr. II on day 5 (>4 µg/mL) and on day

10 (4 µg/mL) as compared to the control. Besides this, alteration of

MIC values were observed in case of penicillin (≤ 0.03 to >8 µg/mL),

vancomycin (≤ 0.2 to >16 µg/mL), nitrofurantoin (≤ 32 to >64 µg/mL),

clindamycin (≤ 0.5 to >2 µg/mL), ampicillin (≤ 0.25 to >8 µg/mL) and

ampicillin/sulbactam (≤ 8/4 to >16/8 µg/mL) in Gr. II on day 5 and 10

as compared to the control. e MIC value of tetracycline was changed

from ≤ 4 to 8 µg/mL in Gr. II on day 10 and MIC value of oxacillin

was changed from ≤ 0.25 to >2 µg/mL (on day 5) and 2 µg/mL on day

10 in Gr. II as compared to control. Antimicrobials did not show any

change in MIC value in Gr. III as compared to the control aer bioeld

treatment. Seventeen out of twenty seven (62.96%) antimicrobials

showed alteration of MIC value in Gr. II on day 5 and 66.67% (eighteen

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Effect of Bioeld Energy Treatment on Streptococcus group B: A

Postpartum Pathogen. J Microb Biochem Technol 7: 269-273. doi:10.4172/1948-5948.1000223

Volume 7(5): 269-273 (2015) - 271

ISSN: 1948-5948 JMBT, an open access journal

such as ranose (RAF) and sorbitol (SOR) showed an alteration of

biochemical reaction i.e. negative (-) to positive (+) in Gr. II on day 5

while remained negative in Gr. II on day 10 and in Gr. III as compared

e one of the key characteristic feature for GBS is complete

lysis of red blood cells and metabolization of sugars by fermentation

process. Hence, in control sample the positive reaction of hemolysin

(HEM) indicated β-hemolysis of erythrocytes and positive reaction of

acidifying lactose (LAC) indicated the production of lactic acid as bi-

product. e data was supported with literature [23]. Similarly, based

on existing literature pyrolidonyl arylamidase (PYR) has negative

reaction in group B streptococcal species while positive reaction in

group A streptococcal. Control data of PYR was well supported with

literature data [24]. However, aer bioeld treatment the negative

reaction was altered in Gr. II, assessed on day 5 as well as day 10 due to

change of enzymatic reaction.

Overall, 48.28% biochemical reactions were altered in tested

twenty-nine biochemicals with respect to the control aer bioeld

treatment. In both time points of Gr. II (day 5 and 10) 41.38% (twelve

out of twenty-nine) biochemical reactions were altered as compared

to the control. About 51.72% out of twenty-nine biochemicals, such as

arabinose (ARA), bacillosamine (BAC), β-lactamase (BL), hemolysin

(HEM), indoxyl phosphatase (IDX), inulin (INU), acidication lactose

(LAC), mannose (MNS), micrococcus screen (MS), optochin (OPT),

glycosidase (PGT), phosphatase (PHO), thymidine free growth (TFG),

acidication trehalose (TRE), and Voges-Proskauer (VP) did not

out of twenty seven) on day 10 as compared to the control aer bioeld

treatment. Eight, out of twenty seven tested antimicrobials (29.63%)

viz. cefepime, ciprooxacin, gatioxacin, imipenem, levooxacin,

moxioxacin, ooxacin and piperacillin/tazobactam did not show any

alteration of MIC values in all the treated cells of GBS as compared to

Biochemical reactions studies

Study of biochemical reactions can be utilized to identify

the enzymatic and metabolic characteristic features of microbes.

Microorganisms can be categorically dierentiated based on their

utilization of specic biochemicals as nutrients during the process of

metabolism or enzymatic reactions. e specic biochemical showed

some changes against S. agalactiae gr. Baer bioeld treatment that

are shown in Table 2. Biochemicals such as bile esculin (BE), crystal

violet (CV), mannitol (MAN), sodium chloride (NaCl), nitrate (NIT),

novobiocin (NOV), pyrolidonyl arylamidase (PYR) and urea (URE)

were changed from negative (-) to positive (+) reactions in revived

treated group (Gr. II) on day 5 and 10, but remained same i.e. negative

(-) reaction in lyophilized treated cells (Gr. III) with respective to

control. Arginine (ARG) and glycosidase (PGR) were converted

from positive (+) to negative (-) reactions in Gr. II on day 10, while

remained unchanged i.e. positive (+) on day 5 in Gr. II and in Gr. III as

compared to the control in bioeld treated S. agalactiae gr. B. Similarly,

pyruvate (PRV), and rambose (RBS) were converted from positive (+)

to negative (-) reactions on both days in Gr. II, while did not show any

change in Gr. III as compared with the control. Moreover, biochemicals

S. No.AntimicrobialType of Response

10. Ciprooxacin≤1 ≤1 ≤1 ≤1

12. Gatioxacin≤2 ≤2 ≤2 ≤2

14. Levooxacin≤2 ≤2 ≤2 ≤2

16. Moxioxacin≤2 ≤2 ≤2 ≤2

22. Piperacillin/tazobactam≤4 ≤4 ≤4 ≤4

MIC values are presented in µg/mL; Gr.: Group

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Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

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Study more about the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

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Study Mahendra Kumar Trivedi research, to understand the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity and biochemical reactions.

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Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen

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Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with

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The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

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¹ Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

² Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Madhya Pradesh, India

*Corresponding Author:

Snehasis Jana
Trivedi Global Inc.
10624 S Eastern Avenue Suite A-969
Henderson, NV 89052, USA
Tel: +91-755-6660006
E-mail: [email protected]

Received Date: August 12, 2015; Accepted Date: September 01, 2015; Published Date: September 08, 2015

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen. J Microb Biochem Technol 7:269-273. doi:10.4172/1948-5948.1000223

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Microbial & Biochemical Technology

Abstract

Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains of S. agalactiae gr. B were selected for the study. Gr. I was considered as control. Both revived (Group; Gr. II) and lyophilized (Gr. III) strains of S. agalactiae gr. B were subjected to Mr. Trivedi's biofield treatment. Gr. II was assessed on day 5 and day 10 while Gr. III on day 10 with respect to the control (Gr. I) using MicroScan Walk-Away® system. Although biofield treatment did not show any change with respect to susceptibility pattern. However the minimum inhibitory concentration of S. agalactiae gr. B showed significant (70.37%) alteration, out of twenty-seven tested antimicrobials, among which in Gr. II i.e. 62.96% on day 5 and 66.67% on day 10 while no alteration was found in lyophilized group (Gr. III) as compared to the control. Moreover, the improvement of MIC value of norfloxacin was observed by two-fold (8 to ≤4 μg/mL) in Gr. II on day 10 after biofield energy treatment as compared to the control. It was observed that overall 48.28% biochemical reactions, out of twenty-nine were altered in Gr. II with respect to the control. Moreover, biotype numbers were changed in Gr. II on day 5 (777777615) and on day 10 (757677405) as compared to the control (237147047). The results suggest that biofield treatment has significant impact on S. agalactiae gr. B in revived treated cells (Gr. II) with respect to MIC values, biochemical reactions pattern and biotype number.

Keywords

Streptococcus agalactiae group B; Biofield treatment; Minimum inhibitory concentration; Biochemical reaction; Biotype

Abbreviations

CLSI: Clinical and laboratory standards institute; GBS: Group B Streptococci; CAMP: Christie-Atkins-Munch-Petersen; CDC: Centers for Disease Control and Prevention; ACOG: American College of Obstetricians and Gynecologists; AAP: American Academy of Pediatrics; MIC: Minimum inhibitory concentration; ATCC: American Type Culture Collection; PBPC 20: Positive Breakpoint Combo 20

Introduction

Group B Streptococci (GBS), or Streptococcus agalactiae group B, are Gram-positive, non-spore forming, non-motile, β-hemolytic and chain-forming cocci bacteria. It is mainly inhabitant in human gut flora and female urogenital tract [ 1]. Pregnant women [ 2] and neonates [ 3] are the main victim host of this organism. It can be transferred to neonates through the birth canal and causes bacterial septicaemia and neonatal meningitis [ 4]. Most of GBS, produce Christie-Atkins-Munch-Petersen (CAMP) factor or protein B an extracellular cytolytic protein and β-lysin from Staphyllococcus species are jointly lysed the erythrocytes [ 5]. It is estimated that in US over 70,000 cases of GBS diseases were prevented [ 6]. Several antibiotics such as penicillin, ampicillin, amoxicillin, cephalosporins (all three generations), macrolides, clindamycin and vancomycin (as alternative) have been used to treat against GBS infections. Among above mentioned antibiotics penicillin is the drug of choice next to cefazolin. However, it also have certain limitations such as high minimum inhibitory concentration (MIC), a factor associated with high level of bacteremia and more concentration of microbes in tissue, especially in cerebrospinal fluid [ 7, 8]. Therefore, some alternative treatment strategies are needed to overcome these lacunas against β-hemolytic strain of gr. B Streptococci. Biofield treatment has been known as an alternative approach that may be useful for S. agalactiae group B infected patients.

Researchers have shown that short-lived electrical events or action potential exist in the several type of mammalian cells such as neurons, muscles, and endocrine cells [ 9]. For instance, when the cells present in central nervous system of human body communicate with each another by means of electrical signals that propagate along the nerve impulses. Therefore, it was hypothesized that biofield exists around the human body and evidence was found using electromyography, electrocardiography and electroencephalogram [ 10]. Thus, the human body emits the electromagnetic waves in the form of bio-photons, which surrounds the body and it is commonly known as biofield. Therefore, the biofield consists of electromagnetic field, being generated by moving electrically charged particles (ions, cell, molecule etc.) inside the human body. Rivera-Ruiz reported that electrocardiography has been extensively used to measure the biofield of human body [ 11]. Thus, human has the ability to harness the energy from environment or Universe and can transmit into any living or nonliving object(s) around the Globe. The objects always receive the energy and responding into useful way that is called biofield energy and the process is known as biofield treatment that is also called as Trivedi effect ®. Mr. Trivedi's unique biofield treatment has been known to transform the structural, physical, and thermal properties of several metals in material science [ 12- 14], improved the overall productivity of crops [ 15, 16], altered characteristics features of microbes [ 17- 19] and improved growth and anatomical characteristics of various medicinal plants [ 20, 21].

Due to the clinical significance of this organism and literature reports on biofield treatment, the present work was undertaken to evaluate the impact of biofield treatment on S. agalactiae group B in relation to antimicrobials susceptibility and bio typing based on various biochemical characters.

Materials and Methods

S. agalactiae group B, American Type Culture Collection (ATCC 12386) strains were procured from MicroBioLogics, Inc., USA, in two sets A and B. Two different sealed packs were stored with proper storage conditions until further use. All the tested antimicrobials and biochemicals were procured from Sigma-Aldrich, MA, USA. The antimicrobial susceptibility, biochemical reactions and biotype number were estimated with the help of MicroScan Walk-Away ® (Dade Behring Inc., West Sacramento, CA, USA) using Positive Breakpoint Combo 20 (PBPC 20) panel with respect to the control group.
Experimental design
Two ATCC samples A and B of S. agalactiae gr. B were grouped (Gr.). ATCC A sample was revived and divided into two parts Gr.I (control) and Gr.II (revived); likewise, ATCC B was labeled as Gr.III (lyophilized).
Biofield treatment strategy
The Gr. I remained as untreated. The treatment Gr. II and III in sealed pack were handed over to Mr. Trivedi for biofield energy treatment under laboratory condition. Mr. Trivedi provided the treatment through his energy transmission process which includes bioenergy emission to the treated groups (Gr. II and Gr. III) without touching the samples. After treatment, sample was handed over in the same condition and stored at standard conditions as per the standard experimental protocol. An optimum precautionary measure were taken while evaluating the study parameters throughout the experiments. The differences in parameters before and after the treatment were noted and compared. Gr.II was assessed at two time point i.e. on day 5 and day 10, while Gr. III was assessed on day 10 for antimicrobial susceptibility, MIC, biochemical reactions pattern, and biotyping.
Antimicrobial susceptibility test
Investigation of antimicrobial susceptibility of S. agalactiae gr. B was carried out with the help of automated instrument, MicroScan Walk-Away ® system using PBPC 20 panel as per the clinical and laboratory standards institute (CLSI) guidelines. The test was carried out on MicroScan, which was miniaturized of the broth dilution susceptibility test that has been dehydrated. Briefly, the standardized suspension of S. agalactiae gr. B was inoculated, rehydrated, and then subjected to incubation for 16 hours at 35°C. The detailed experimental procedures and conditions were followed as per the manufacturer's instructions. The antimicrobial susceptibility pattern and MIC were determined by observing the lowest antimicrobial concentration showing growth inhibition [22].
Biochemical reaction studies
Biochemical reactions of S. agalactiae gr. B were determined using MicroScan Walk-Away ®, system with PBPC 20 panel [ 22]. Biochemicals used in the study were arabinose, arginine, bacillosamine, bile esculin, β-lactamases, crystal violet, hemolysin, indoxyl phosphatase, inulin, acidification lactose, mannitol, mannose, micrococcus screen, sodium chloride, nitrate, novobiocin, optochin, p-nitro phenyl β-Dglucuronide, p-nitro phenyl β-D-galactopyranoside, phosphatase, pyruvate, pyrolidonyl arylamidase, raffinose, rambose, sorbitol, thymidine free growth, acidification trehalose, urea, and Voges- Proskauer.
Identification of organism by biotype number
The biotype number of S. agalactiae gr. B was determined on MicroScan Walk-Away ® processed panel data report with the help of biochemical reactions data [ 22].

Results and Discussion
Antimicrobial susceptibility test
The outcomes of MIC values of tested antimicrobials against S. agalactiae gr. B after biofield treatment are summarized in Table 1. The data were analyzed using automated system and compared with respect to the control. In this experiment, twelve antibiotics were used to investigate the susceptibility pattern on GBS viz. ampicillin, cefepime, cefotaxime, ceftriaxone, chloramphenicol, clindamycin, levofloxacin, linezolid, ofloxacin, penicillin, tetracycline, and vancomycin. Out of these, five antibiotics showed susceptible in the control sample. It has been evidenced from literatures that penicillin and its derivatives are the choice of drugs against gr. B streptococcal infection [8,9]. However, biofield treatment did not show any alteration with respect to susceptibility pattern in all the treated groups after biofield treatment (data not shown). Besides sensitivity assay, the MIC values of tested antimicrobials were significantly (70.37%) altered out of twenty seven as compared to the control. The MIC value of norfloxacin was reduced by two-fold to ≤ 4 μg/mL after biofield treatment in Gr. II on day 10 as compared to the control (8 μg/mL). The MIC values of cefazolin, cephalothin, and chloramphenicol were changed from ≤ 8 to >16 μg/ mL in Gr. II on day 5 and 10 as compared to the control. The MIC values of rifampin and synercid were changed from ≤ 1 to >2 μg/mL in Gr. II on day 5 and 10 as compared to the control. Alteration of MIC values of cefotaxime and ceftriaxone were changed from ≤8 to 32 μg/mL in Gr. II on day 5 and 10 as compared to the control. Moreover, MIC values of amoxicillin/k-clavulanate and trimethoprim/sulfamethoxazole were slightly changed from ≤4/2 to >4/2 μg/mL (on day 5 and 10) and ≤2/38 to >2/38 μg/mL (on day 5) respectively in Gr. II after biofield treatment as compared to the control. Antimicrobial linezolid showed an alteration of MIC value in Gr. II on day 5 (>4 μg/mL) and on day 10 (4 μg/mL) as compared to the control. Besides this, alteration of MIC values were observed in case of penicillin (≤ 0.03 to >8 μg/mL), vancomycin (≤ 0.2 to >16 μg/mL), nitrofurantoin (≤ 32 to >64 μg/mL), clindamycin (≤ 0.5 to >2 μg/mL), ampicillin (≤ 0.25 to >8 μg/mL) and ampicillin/sulbactam (≤ 8/4 to >16/8 μg/mL) in Gr. II on day 5 and 10 as compared to the control. The MIC value of tetracycline was changed from ≤ 4 to 8 μg/mL in Gr. II on day 10 and MIC value of oxacillin was changed from ≤ 0.25 to >2 μg/mL (on day 5) and 2 μg/mL on day 10 in Gr. II as compared to control. Antimicrobials did not show any change in MIC value in Gr. III as compared to the control after biofield treatment. Seventeen out of twenty seven (62.96%) antimicrobials showed alteration of MIC value in Gr. II on day 5 and 66.67% (eighteen out of twenty seven) on day 10 as compared to the control after biofield treatment. Eight, out of twenty seven tested antimicrobials (29.63%) viz. cefepime, ciprofloxacin, gatifloxacin, imipenem, levofloxacin, moxifloxacin, ofloxacin and piperacillin/tazobactam did not show any alteration of MIC values in all the treated cells of GBS as compared to the control ( Table 1).

MIC values are presented in μg/mL; Gr.: Group

Table 1: Effect of biofield treatment on Streptococcus agalactiae group B to minimum inhibitory concentration (MIC) of tested antimicrobials.
Biochemical reactions studies
Study of biochemical reactions can be utilized to identify the enzymatic and metabolic characteristic features of microbes. Microorganisms can be categorically differentiated based on their utilization of specific biochemicals as nutrients during the process of metabolism or enzymatic reactions. The specific biochemical showed some changes against S. agalactiae gr. B after biofield treatment that are shown in Table 2. Biochemicals such as bile esculin (BE), crystal violet (CV), mannitol (MAN), sodium chloride (NaCl), nitrate (NIT), novobiocin (NOV), pyrolidonyl arylamidase (PYR) and urea (URE) were changed from negative (-) to positive (+) reactions in revived treated group (Gr. II) on day 5 and 10, but remained same i.e. negative (-) reaction in lyophilized treated cells (Gr. III) with respective to control. Arginine (ARG) and glycosidase (PGR) were converted from positive (+) to negative (-) reactions in Gr. II on day 10, while remained unchanged i.e. positive (+) on day 5 in Gr. II and in Gr. III as compared to the control in biofield treated S. agalactiae gr. B. Similarly, pyruvate (PRV), and rambose (RBS) were converted from positive (+) to negative (-) reactions on both days in Gr. II, while did not show any change in Gr. III as compared with the control. Moreover, biochemicals such as raffinose (RAF) and sorbitol (SOR) showed an alteration of biochemical reaction i.e. negative (-) to positive (+) in Gr. II on day 5 while remained negative in Gr. II on day 10 and in Gr. III as compared to the control.

'-' (negative); '+' (positive); Gr.: Group; NR: Not reported; *PGR: p-nitro phenyl β-D- glucuronide; #PGT: p-nitro phenyl β-D-galactopyranoside.

Table 2: Effect of biofield treatment on Streptococcus agalactiae group B to the biochemical reaction pattern.

The one of the key characteristic feature for GBS is complete lysis of red blood cells and metabolization of sugars by fermentation process. Hence, in control sample the positive reaction of hemolysin (HEM) indicated β-hemolysis of erythrocytes and positive reaction of acidifying lactose (LAC) indicated the production of lactic acid as biproduct. The data was supported with literature [ 23]. Similarly, based on existing literature pyrolidonyl arylamidase (PYR) has negative reaction in group B streptococcal species while positive reaction in group A streptococcal. Control data of PYR was well supported with literature data [ 24]. However, after biofield treatment the negative reaction was altered in Gr. II, assessed on day 5 as well as day 10 due to change of enzymatic reaction.

Overall, 48.28% biochemical reactions were altered in tested twenty-nine biochemicals with respect to the control after biofield treatment. In both time points of Gr. II (day 5 and 10) 41.38% (twelve out of twenty-nine) biochemical reactions were altered as compared to the control. About 51.72% out of twenty-nine biochemicals, such as arabinose (ARA), bacillosamine (BAC), β-lactamase (BL), hemolysin (HEM), indoxyl phosphatase (IDX), inulin (INU), acidification lactose (LAC), mannose (MNS), micrococcus screen (MS), optochin (OPT), glycosidase (PGT), phosphatase (PHO), thymidine free growth (TFG), acidification trehalose (TRE), and Voges-Proskauer (VP) did not show any change in all the treated groups after biofield treatment as compared to the control.
Identification of organism by biotype number
The species ( S. agalactiae gr. B) was identified based on variety of conventional biochemical characters and biotyping. Biotype number of particular organism was evaluated after interpreting the results of the biochemical reactions. The biotype number then led to the particular organism identification. In this experiment, biotyping was performed using automated systems. Results showed changes of biotype numbers in the biofield treated Gr. II (on day 5 and 10) and Gr. III (on day 10) without alteration of organism. Based on the biochemical data, biotype number was changed in treated Gr. II on day 5 (777777615, S. agalactiae group B) and on day 10 (757677405, S. agalactiae group B) with respect to the control (237147047) i.e. S. agalactiae group B ( Table 3). Biotyping, covers the overall cellular pattern of enzymatic activities of any organism. GBS can be characterized based on analysis of biochemical properties with CAMP, Na-hippurate positive and esculin negative as key characters [ 25]. In this experiment, bile esculin also showed negative (-) reaction in control sample, that directly correlated with literature. This negative reaction of esculin was altered after biofield treatment in revived treated cells of GBS. It was indicated that biofield treatment has the ability to alter the biochemical pattern which may be due to change the enzymatic reaction. So, it is assumed that these changes of biotype number without alteration in organism may be due to change of metabolic and/or enzymatic reactions of GBS.

Table 3: Effect of biofield treatment on biotype number of Streptococcus agalactiae group B.

Biofield treatment may responsible for alteration in microorganism at genetic and/or enzymatic level, which probably act on receptor protein. While altering receptor protein, ligand-receptor/protein interactions may alter that could lead to different phenotypic characteristics [ 26]. Biofield treatment might induce a significant changes in revived strain of GBS and altered the MIC values, biochemical reactions, and ultimately change the biotype number of microorganism.

Conclusion

Altogether, the biofield treatment has significantly altered 70.37%, (out of twenty-seven) the MIC values of tested antimicrobials against the strain of S. agalactiae gr. B. Norfloxacin was improved the MIC value by two-fold (8 to ≤4 μg/mL) in Gr. II on day 10 after biofield energy treatment as compared to the control. Additionally, it also significantly (48.28%) altered the biochemical reactions pattern of biofield energy treated strain of S. agalactiae gr. B. On the basis of utilization of group B streptococcal specific biochemicals, change in metabolic reactions led to variation of biotype number in all the treated groups without change of organism after biofield treatment with respect to the control. Based on above findings, it is assumed that Mr. Trivedi's biofield treatment is an alternative approach to alter the antibiogram profile of S. agalactiae gr. B.

Acknowledgements

Authors gratefully acknowledged to Trivedi science, Trivedi testimonials and Trivedi master wellness and the whole team of PD Hinduja National Hospital and MRC, Mumbai, Microbiology Lab for their support.

References

1. Campbell JR, Hillier SL, Krohn MA, Ferrieri P, Zaleznik DF, et al. (2000) Group B Streptococcal colonization and serotype-specific immunity in pregnant women at delivery. ObstetGynecol 96: 498-503.

2. Cowgill K, Taylor TH Jr, Schuchat A, Schrag S (2003) Report from the CDC. Awareness of perinatal group B streptococcal infection among women of childbearing age in the United States, 1999 and 2002. J Womens Health (Larchmt) 12: 527-532.

3. Mullaney DM (2001) Group B streptococcal infections in newborns. J ObstetGynecol Neonatal Nurs 30: 649-658.

4. van der Poll T, Opal SM (2008) Host-pathogen interactions in sepsis. Lancet Infect Dis 8: 32-43.

5. Ratner HB, Weeks LS, Stratton CW (1986) Evaluation of spot CAMP test for identification of group B streptococci. J ClinMicrobiol 24: 296-297.

6. Schuchat A, Wenger JD (1994) Epidemiology of group B streptococcal disease. Risk factors, prevention strategies, and vaccine development. Epidemiol Rev 16: 374-402.

7. Jacobs MR, Kelly F, Speck WT (1982) Susceptibility of group B streptococci to 16 beta-lactam antibiotics, including new penicillin and cephalosporin derivatives. Antimicrob Agents Chemother 22: 897-900.

8. McCracken GH Jr, Ginsberg C, Chrane DF, Thomas ML, Horton LJ (1973) Clinical pharmacology of penicillin in newborn infants. J Pediatr 82: 692-698.

9. Myers R (2003) The basics of chemistry. Greenwood Press, Westport, Connecticut.

10. Movaffaghi Z, Farsi M (2009) Biofield therapies: biophysical basis and biological regulations? Complement TherClinPract 15: 35-37.

11. Rivera-Ruiz M, Cajavilca C, Varon J (2008) Einthoven's string galvanometer: the first electrocardiograph. Tex Heart Inst J 35: 174-178.

12. Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28.

13. Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.

14. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.

15. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J AgricSci 35: 22-29.

16. Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 83: 138-143.

17. Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.

18. Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiellaoxytoca: An impact of biofield treatment. J MicrobBiochemTechnol 7: 203-206.

19. Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderiacepacia: A multihost pathogen. J Trop Dis 3: 167.

20. Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemoncablin (Benth.). Biotechnology 11: 154-162.

21. Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.

22. Fader RC, Weaver E, Fossett R, Toyras M, Vanderlaan J, et al. (2013) Multilaboratory study of the biomic automated well-reading instrument versus MicroScanWalkAway for reading MicroScan antimicrobial susceptibility and identification panels. J ClinMicrobiol 51: 1548-1554.

23. Kaufhold A, Ferrieri P (1993) The microbiologic aspects, including diagnosis, of beta-hemolytic streptococcal and enterococcal infections. Infect Dis Clin North Am 7: 235-256.

24. Facklam RR, Thacker LG, Fox B, Eriquez L (1982) Presumptive identification of streptococci with a new test system. J ClinMicrobiol 15: 987-990.

25. Ekin IH, Gurturk K, Yaman G, Yesilova A (2010) Biotyping of nontypeable Group B Streptococci. Van Vet J 21: 117-122.

26. Lindstrom E, Mild KH, Lundgren E (1998) Analysis of the T cell activation signaling pathway during ELF magnetic field exposure, p56lck and [Ca2+]i-measurements. BioeletrochemBioenerg 46: 129-137.

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Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen

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1 Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA 2 Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Madhya Pradesh, India *Corresponding Author: Snehasis Jana Trivedi Global Inc.

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	 An Effect of Biofield Treatment on Hydrolysate & Ipomoea Macroelements
	

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	 An Effect of Biofield Treatment on Hydrolysate & Ipomoea Macroelements
	

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To know more about the effect of Biofield Treatment on physical and thermal properties of gluten hydrolysate and ipomoea macroelements, then Read this research done by Mahendra Kumar Trivedi.

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	Biofield | Impact on Gluten Hydrolysate and Ipomoea Macroelements
	

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	Biofield | Impact on Gluten Hydrolysate and Ipomoea Macroelements
	

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Read the clinical study on the impact of biofield treatment on physical and thermal properties of gluten hydrolysate and ipomoea macroelements.

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Analytical & Bioanalytical

Trivedi et al.,J Anal Bioanal Tech 2015, 6:5

http://dx.doi.org/10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

Keywords: Disulram; Nicotinic acid; Bioeld treatment; Fourier

transform infrared spectroscopy; Ultraviolet spectroscopy

Disulram [bis(diethylthiocarbamoyl)disulphide] is an antabuse

drug, being used clinically as an aid to the treatment of chronic

alcoholism. It is the rst drug approved by US Food and Drug

Administration to treat the alcohol addiction [1]. Alcohol (ethanol)

transforms into acetaldehyde by alcohol dehydrogenase enzyme, which

further oxidized to acetic acid by acetaldehyde dehydrogenase (ADH)

enzyme [2]. Disulram inhibits the ADH enzyme. As a result, the blood

concentration of acetaldehyde increases and causes an unpleasant

eect, thus increase the patient's motivation to remain abstinent [3]. In

addition to this, disulram is reported for protozoacidal eect in vitro

study [4,5]. Recently, disulram has shown the reactivity to latent HIV-

1 expression in a primary cell model of virus latency and presently it is

assessed in a clinical trial for its potential to diminish the latent HIV-1

reservoir in patients combination with antiretroviral therapy [6].

Nicotinic acid or niacin is one of the B-complex vitamins (Vitamin

B3 ) that has cholesterol lowering activity. Recent studies showed that

therapeutic doses of nicotinic acid induce a profound alteration in

plasma concentration of several lipids and lipoproteins, resulting in a

greater ability to increase high-density lipoprotein (HDL) cholesterol

[7]. Nicotinic acid favorably aects apolipoprotein (apo), very-low-

density lipoprotein (VLDL), low-density lipoprotein (LDL) and HDL

e exact mechanism of nicotinic acid activity is unknown.

However, new ndings indicate that nicotinic acid inhibits directly

and non-competitively to the triglycerides synthesis enzyme i.e.,

hepatocyte diacylglycerol acyltransferase-2, which causes acceleration

of intracellular hepatic apo B degradation and thus decrease secretion

of VLDL and LDL [9]. Several evidence suggest that nicotinic acid

administered either alone or in combination with other cholesterol-

lowering medicines can reduce the risk of cardiovascular and

atherosclerosis diseases. e clinical uses of nicotinic acid are

somewhat limited due to some harmless but unpleasant side eects

like cutaneous ushing phenomenon, nausea, vomiting and headache

[10]. e chemical and physical stability of pharmaceutical drugs or

products are most desired attributes of quality that potentially aect the

ecacy, safety and shelf life of drugs [11]. Hence, it is essential to nd

out an alternate approach, which could enhance the stability of drugs

by altering the structural and bonding properties of these compounds.

Contemporarily, bioeld treatment is reported to alter the spectral

properties of various pharmaceutical drugs like paracetamol, piroxicam,

metronidazole, and tinidazole; likewise physical, and structural

properties of various metals i.e., tin, lead etc. [12-14]. e conversion of

mass into energy is well known in literature for hundreds of years that

was further explained by Hasenohrl and Einstein [15,16]. According to

Maxwell JC, every dynamic process in the human body had an electrical

signicance, which generates magnetic eld in the human body [17].

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd, Bhopal-462026,

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Bairwa K, et al. (2015)

Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Disulram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex

vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of bioeld

treatment on spectral properties of disulram and nicotinic acid. The study was performed in two groups i.e., control

and treatment of each drug. The treatment groups were received Mr. Trivedi's bioeld treatment. Subsequently,

spectral properties of control and treated groups of both drugs were studied using Fourier transform infrared (FT-IR)

and Ultraviolet-Visible (UV-Vis) spectroscopic techniques. FT-IR spectrum of bioeld treated disulram showed the

shifting in wavenumber of C-H stretching from 1496 to 1506 cm-1 and C-N stretching from 1062 to 1056 cm-1. The

intensity of S-S dihedral bending peaks (665 and 553 cm-1 ) was also increased in bioeld treated disulram sample,

as compared to control. FT-IR spectra of bioeld treated nicotinic acid showed the shifting in wavenumber of C-H

stretching from 3071 to 3081 cm-1 and 2808 to 2818 cm-1. Likewise, C=C stretching peak was shifted to higher

frequency region from 1696 cm-1 to 1703 cm-1 and C-O (COO- ) stretching peak was shifted to lower frequency region

from 1186 to 1180 cm-1 in treated nicotinic acid.

UV spectrum of control and bioeld treated disulram showed similar pattern of UV spectra. Whereas, the UV

spectrum of bioeld treated nicotinic acid exhibited the shifting of absorption maxima (λmax ) with respect of control

i.e., from 268.4 to 262.0 nm, 262.5 to 256.4, 257.5 to 245.6, and 212.0 to 222.4 nm.

Over all, the FT-IR and UV spectroscopy results suggest an impact of bioeld treatment on the force constant,

bond strength, and dipole moments of treated drugs such as disulram and nicotinic acid that could led to change in

their chemical stability as compared to control.

Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Khemraj Bairwa2 and Snehasis Jana2 *

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disulram and Nicotinic Acid after

Bioeld Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

is electromagnetic eld of the human body is known as bioeld and

energy associated with this eld is known as bioeld energy [18,19].

Mr. Trivedi has the ability to harness the energy from environment or

universe and can transmit into any living or nonliving object around

this Globe. e object(s) always receive the energy and responding into

useful way, this process is known as bioeld treatment.

Mr. Mahendra Kumar Trivedi's bioeld treatment (e Trivedi

Eect®) has considerably changed the physicochemical, thermal and

structural properties of metals and ceramics [14,20,21]. Growth and

anatomical characteristics of some plants were also increased aer

bioeld treatment [22,23]. Further, bioeld treatment has showed

the signicant eect in the eld of agriculture science [24,25] and

Considering the impact of bioeld treatment on physical and

structural property of metals and ceramics, the present study was

aimed to evaluate the impact of bioeld treatment on spectral

properties of disulram and nicotinic acid. e eects were analyzed

using Fourier transform infrared (FT-IR) and Ultraviolet-Visible (UV-

Vis) spectroscopic techniques.

e disulram and nicotinic acid (Figure 1) samples were procured

from Sigma-Aldrich, MA, USA; and each drug was divided into two

parts i.e., control and treatment. e control samples were remained

as untreated, and treatment samples were handed over in sealed pack

to Mr. Trivedi for bioeld treatment under laboratory condition.

Mr. Trivedi provided this treatment through his energy transmission

process to the treated groups without touching the sample [12,13].

e control and treated samples of disulram and nicotinic acid were

evaluated using FT-IR and UV-Vis spectroscopy.

FT-IR spectroscopic characterization

FT-IR spectra were recorded on Shimadzu's Fourier transform

infrared spectrometer (Japan) with frequency range of 4000-500 cm-1 .

e FT-IR spectroscopic analysis of both control and treated samples

of disulram and nicotinic acid were carried out to evaluate the impact

of bioeld treatment at atomic level like force constant and bond

UV-Vis spectroscopic analysis

UV spectra of disulram and nicotinic acid were recorded on

Shimadzu UV-2400 PC series spectrophotometer with 1 cm quartz

cell and a slit width of 2.0 nm. e analysis was carried out using

wavelength in the range of 200-400 nm. e analysis was performed

to determine the eect of bioeld treatment on structural properties of

FT-IR spectroscopic analysis

Vibrational spectral assignment was performed on the recorded

FT-IR spectra (Figure 2) based on theoretically predicted wavenumber

and presented in Table 1. e FT-IR spectrum of control disulram

sample (Figure 2a) showed the characteristic vibrational peak at 2975

peak observed at 1496 cm-1 was attributed to C-H symmetrical

deformation vibrations. e absorption peaks appeared at 1351-1457

cm-1 was assigned to CH2 -CH3 deformations. e vibrational peaks

at 1273 cm-1 and 1151-1195 cm-1 were assigned to C=S stretching and

C-C skeletal vibration, respectively. Further, IR peaks observed at 967-

1062 cm-1 and 818-914 cm-1 were attributed to C-N stretching and C-S

stretching, respectively. e vibrational peaks appeared at 554-666

cm-1 was assigned to S-S dihedral bending. e FT-IR data of control

disulram was well supported by the literature data [29].

e FT-IR spectrum of bioeld treated disulram (Figure 2b)

showed the vibrational peak at 2975 cm-1 ,which was assigned toCH3

stretching. Vibrational peak appeared at 1506 cm-1 was assigned to

C-H symmetrical deformation vibrations. Likely, the IR peaks at 1350-

1457 cm-1 were attributed to CH2 -CH3 deformations. e vibrational

peaks appeared at 1273 cm-1 and 1151-1195 cm-1 were assigned to

C=S stretching and C-C skeletal vibration, respectively. e IR peaks

observed at 967-1056 cm-1 and 817-914 cm-1 were attributed to C-N

stretching and C-S stretching, respectively. e vibrational peaks at

553-665 cm-1 were assigned to S-S dihedral bending.

Altogether, the FT-IR data of bioeld treated disulram (Figure 2b)

showed the shiing in frequency of some bonds with respect to control

spectra like C-H symmetrical deformation vibrations frequency was

shied from 1496 (control) to 1506 (treated) cm-1. e frequency (ν)

of vibrational peak depends on two factors i.e., force constant (k) and

Figure 1: Chemical structure of (a) Disulram and (b) Nicotinic acid.

Figure 2: FT-IR spectra of Disulram (a) control and (b) treated.

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al. (2015) Spectroscopic Characterization of Disulram and Nicotinic Acid after

Bioeld Treatment. J Anal Bioanal Tech 6: 265 doi:10.4172/2155-9872.1000265

Volume 6 * Issue 5 * 1000265

ISSN: 2155-9872 JABT, an open access journal

reduced mass (μ), which can be explained by following equation [30]

here, c is speed of light.

If reduced mass is constant, then the frequency is directly

proportional to the force constant; therefore, increase in frequency

of any bond suggested a possible enhancement in force constant of

respective bond and vice versa [28]. Based on this it is hypothesized

that due to increase in frequency of C-H symmetrical deformation

(1496 to 1506 cm-1), the C-H bond strength in treated disulram

might also be increased with respect of control. Contrarily, the C-N

stretching vibration in treated disulram was sied to lower frequency

region as compared to control i.e., from 1062 to 1056 cm-1. is could

be referred to decrease in C-N bond strength aer bioeld treatment in

compression to control. In addition, the intensity of IR peak appeared

at 553-665 cm-1 (S-S dihedral bending) in bioeld treated sample

was found to be increased, with respect of control peaks in the same

frequency region. e intensity of vibrational peaks of particular bond

depends on the ratio of change in dipole moment (∂µ) to change in bond

distance (∂r) i.e., the intensity is proportionally change with changes in

dipole moment and inversely change with alteration in bond distance

[31]. Based on this, it is speculated that ratio of ∂µ/∂r might be altered

in S-S bonds (appeared in the frequency region of 553-665 cm-1) with

the inuence of bioeld treatment as compared to control.

e vibrational spectral assignment of nicotinic acid was performed

on the recorded FT-IR spectra (Figure 3) based on theoretically

predicted wavenumber and presented in Table 2. e vibrational peaks

appeared at 3071-2808 cm-1 was assigned to C-H stretchings. e IR

peaks observed at 1696-1710 cm-1 and 1596 cm-1 were assigned to C=O

(COO- ) asymmetrical stretching and C=C stretching, respectively.

Absorption peaks appeared at 1417, 1323, and 1301 cm-1 were

attributed to C=N symmetric stretching, C=O symmetrical stretching,

and C-N stretching, respectively. e C-O (COO-) stretching peak

was assigned to IR bend observed at 1186 cm-1. Further, C-H in plane

and out plane bending vibrations was assigned to peaks observed in

the range of 1033-1114 cm-1 and 642-812 cm-1, respectively. e FT-IR

data of control nicotinic acid was well supported by the literature data

e FT-IR spectrum of bioeld treated nicotinic acid (Figure 3)

showed the absorption bands at 2818-3081 cm-1 that were assigned

to C-H stretching. Vibrational peaks appeared at 1703-1714 cm-1

and 1594 cm-1 were assigned to C=O (COO-) asymmetric stretching

and C=C stretching, respectively. Likewise, the IR peaks observed

at 1417, 1324, and 1301 cm-1 were assigned to C=N stretching, C=O

(COO- ) symmetric stretching, and C-N stretching, respectively. e IR

stretching. Further, the C-H in plane and out plane bending vibrations

Overall, the FT-IR data of biofield treated nicotinic acid (Figure

3) showed the shifting in wavenumber of some bonds with respect

to control sample. For instance, the C-H stretching towards higher

frequency region i.e., from 3071 to 3081 cm-1 and 2808 to 2818 cm-

1. This could be due to increase in force constant of C-H bond.

Likewise, a slight upstream shifting in C=O stretching peak from

1710 to 1714 cm-1 and 1696 to 1703 cm-1 in treated nicotinic acid

also suggests an increase in force constant of C=O bond in treated

sample as compared to control. Contrarily, a slight downstream

shifting in wavenumber of treated nicotinic acid from 1186 to 1180

cm-1 C-O (COO- stretching); and from 1033 to 1037 cm-1 (=C-H

in plane bending) suggests the decrease in force constant of C-O

bond and decrease in rigidity of =C-H bond in treated sample as

14961506C-H symmetrical deformation vibrations

1151-11951151-1195C-C skeletal vibrations

967-1062967-1056C-N stretching

818-914817-914C-S stretching

554-666553-665S-S stretching

Table 1: FT-IR vibrational peaks observed in Disulram.

Figure 3: FT-IR spectra of Nicotinic acid (a) control and (b) treated.

2808-30712818-3081C-H stretching

1696-17101703-1714C=O (COO-) asymmetric stretching

11861180C-OH (Ph-OH) stretching

1033-11141037-1116=C-H in-plane bending

642-812642-812=C-H out of plane bending

Table 2: FT-IR vibrational peaks observed in Nicotinic acid.

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Disulfiram and Nicotinic Acid after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Disulfiram is being used clinically as an aid in chronic alcoholism, while nicotinic acid is one of a B-complex vitamin that has cholesterol lowering activity. The aim of present study was to investigate the impact of biofield treatment on spectral properties of disulfiram and nicotinic acid.

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	Gluten Hydrolysate & Ipomoea Macroelements Properties – Read more
	

# data[].attributes.subject
	
	
	
	
	Gluten Hydrolysate & Ipomoea Macroelements Properties – Read more
	

# data[].attributes.body
Read more about the effect of biofield treatment on the Physical and Thermal Properties of Gluten Hydrolysate and Ipomoea Macroelements.

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"Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr.

# data[].attributes.headline
	
	
	
	
	
		Read Research on Gluten Hydrolysate & Ipomoea Macroelements
	

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		Read Research on Gluten Hydrolysate & Ipomoea Macroelements
	

# data[].attributes.body
A Potential Strategy by Mahendra Kumar Trivedi for Modification of Physical and Thermal Properties of Gluten Hydrolysate and Ipomoea Macroelements.

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	Biofield Treatment Effect on Gluten Hydrolysate & Ipomoea Macroelements
	

# data[].attributes.subject
	
	
	
	
	Biofield Treatment Effect on Gluten Hydrolysate & Ipomoea Macroelements
	

# data[].attributes.body
Research done by Mahendra Kumar Trivedi - The objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate & ipomoea macroelements.

# data[].attributes.attachment_link.title
Bio-field Treatment: A Potential Strategy for Modification of Physical and Thermal Properties of Gluten Hydrolysate and Ipomoea Macroelements

# data[].attributes.attachment_link.description
The objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate (GH) and ipomoea macroelements (IM). The study was performed in two groups (control and treated). The control group

# data[].attributes.headline
	
	
	
	
	Modification Properties of Gluten Hydrolysate & Ipomoea Macroelements
	

# data[].attributes.subject
	
	
	
	
	Modification Properties of Gluten Hydrolysate & Ipomoea Macroelements
	

# data[].attributes.body
Main objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate and ipomoea macroelements.

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	Gluten Hydrolysate & Ipomoea Macroelements | Publication
	

# data[].attributes.subject
	
	
	
	
	Gluten Hydrolysate & Ipomoea Macroelements | Publication
	

# data[].attributes.body
The objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate (GH) and ipomoea macroelements (IM).

# data[].attributes.headline
	
	
	
	
	Antimicrobial Susceptibility Test of Biofield Treated S. Aureus
	

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	Antimicrobial Susceptibility Test of Biofield Treated S. Aureus
	

# data[].attributes.body
Biofield treatment has successfully showed an alteration of antimicrobial susceptibility pattern of Staphylococcus Aureus. Visit to know more about biofield treated Staphylococcus aureus and results.

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	 Study of Biochemical Reactions Pattern of Biofield Treated S. Aureus
	

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	 Study of Biochemical Reactions Pattern of Biofield Treated S. Aureus
	

# data[].attributes.body
Biochemical reactions pattern of biofield treated S. aureus were studied in this research. The study was aimed to evaluate the impact of biofield treatment so that it could be used as an alternative to drug therapy in future.

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Published online October 15, 2015 (http://www.sciencepublishinggroup.com/j/ajbio)

doi: 10.11648/j.ajbio.20150306.13

ISSN: 2330-0159 (Print); ISSN: 2330-0167 (Online)

Antibiogram, Biochemical Reactions and Genotyping

Characterization of Biofield Treated Staphylococcus aureus

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd.,

Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana. Antibiogram, Biochemical

Vol. 3, No. 6, 2015, pp. 212-220. doi: 10.11648/j.ajbio.20150306.13

Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable

(ATCC 25923) was divided into two parts, Gro up (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was

further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed

on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 ( Study II), and divided into three

separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the

experimental parameters were studied using automated MicroScan Walk-Away

system. The 16S rDNA sequencing was

carried out in Gr. II A sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. T he

antimicrobial susceptibility and minimum inhibitory conc entration showed sig nificant alteration i.e. 92.8 6% and 90.00%

respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant

(35.71%) alteration in treated sample with respect to control. The biotype number and microb ial species were substantially

changed in rest of the tr eated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the

identified strain i n this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the

nearest homolog genus-species was found as Staphylococcus simiae (GenBank Access ion No.: DQ127902). These results

suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.

Biotype, 16S rDNA, Gram-Positive Bacteria

Staphylococci ar e the importa nt class of pyogenic Gra m-

structure. They are considered as the thi rd most important

cause of food-borne disorder s in the world [1]. It is the

main pa thogen for masti tis in the milch animals [2]. It is

estimated th at in US alone food-borne illnesses affect 6 to

80 million pe ople each yea r, causing up to 9000 deaths [3].

Based on literature various genes have been found as a

target for identifi cation o f S. aureus with the help of 16S

superoxide dismutase A (sodA) [5], and RNA polymerase B

classes of the antimicrobial agents. Pe nicillin is the drug of

choice to treat against Staphylococcus infection b ut due to

Therefore, some alternative str ategies are needed to treat

against staphylococci infections.

Complementary and Alternative Medicine (NIH/NCC AM)

electromagnetic b ased energy ther apies used to promote

American Journal of BioScience 2015; 3(6): 212-220 213

health and healing [8]. B iofield en ergy treatment has been

known as an alterna tive approach that may be useful to a lter

the sensitivit y pattern of the antimicrobia ls. Harold Saxton

Burr had performed the detailed studies on the correlation

suggested that every si ngle process in the human body had

an electrical signifi cance [9]. Theelec trical process that

happening in t he human bod y have strong relationship with

magnetic field a s requir ed by Ampere's law, whic h stated

surrounding space [ 10, 11]. Thus, the human body emits t he

electromagnetic waves in the form of bio-photons, which

surrounds the body and it is commo nly known as biofi eld.

Therefore, the biofield co nsists of a n electromagnetic field,

being generated by mo ving electrically c harged particles

(ions, cell, molecule, etc.) inside the human body. Prakash

et al. 2015, repo rted that the vari ous scientific instruments

extensively used to measure t he biofield of human body

[12]. Th us, a human has the ability to harne ss the energy

from environment or univer se and can transmit into any

living or nonliving obje ct(s) around the Globe. The objects

always receive the energy and respond into useful way that

biofield treatment. Mr. Trivedi's biofiel d treatment (T he

) has been kno wn to alter the structural,

materials science [ 13-15], improved the overall productivity

characteristics of various medicinal plants [21, 22]. Due to

25923) strain was procure d from Mic roBioLogics, Inc.,

USA and stored with proper st orage conditions until further

use. All the tested antimicr obials and biochemicals we re

biotype number were estimated with the help of Micr oScan

(Dade Behring Inc., West Sacramento, CA,

panel. The 16S r DNA sequencin g analysis was c arried out

(Bangalore Genei, Ind ia).

The impact of biofield treatment on tested bacterium S.

aureus was evaluated in two groups-

Group I: ATCC strain in lyophilized state was considered

antimicrobial sensitivity, biochemical reactions a nd biotype

number as per the standard protocol.

divided into two parts named as Gr. IIA and Gr. IIB. Both the

groups of ATCC strainof S. aureus in lyophilized state were

assigned to the Mr. Trivedi's unique biofield treatment (first

treatment). Gr. IIA was analyzed on day 10 while Gr. IIB

sample was stored in lyophil ized state for 159 days at -70ºC.

Gr. IIB was further sub-divided in two separate parts named

as Gr. IIB - Study Iand Gr. IIB - Study II.

biochemical reactions and biotyping were performed as per

The stored strai n was revived fro m -70ºC and the revived

retreatment, the s ample was sub-cult ured into three separate

tubes and analyzed on Day 5, Day 10 and Day 15 of its sub-

2.2. Biofield Treatment Strategy

The lyophilized sample of S. au reus was subjected to Mr.

retreatment on 159 days in revived state (Gr. IIB, Study I I).

In details, the treatment groups in se aled pack were handed

over to Mr. Trivedi for bio field treatmen t under laboratory

conditions. Mr. Trivedi provided the trea tment through his

energy transmission process to t he treated group without

touching the samples. After first tre atment, the analysis of

antimicrobial sensitivity along with mini mum inhibitory

concentration (MI C), biochemical re actions with bio type

protocol. W hile handing over these culture s to Mr. Trivedi

for retrea tment purp oses, op timum prec autions wer e taken

2.3. Antimicrobial Susceptibility Test

Investigation of antimicr obial susce ptibility of S. aureus

was carried o ut with the h elp of automa ted instrument,

using PBPC 20 panel. The panel

can be stored at 2 to 25ºC for analysis. T he panel was

rehydration. All opened panels were used on the same day.

The tests carried o ut on MicroScan were miniaturized of the

broth dil ution susc eptibility test that has been dehydrated.

Briefly, 0.1 mL of the stan dardized suspens ion of S. aureus

was pipetted into 25 mL of inoculum water using pluro nic

and inverted 8 t o 10 times and inoculated, rehydrated, and

Rehydration and inocu lation were perfor med using the

214 Mahendra Kumar Trivedi et al.: Antibiogram, Biochemical Reactions and Genotyping

Characterization of Biofield Treated Staphylococcus aureus

system with inoculators-D (B1013-4). 25 mL of

inoculum tray. The detailed exp erimental proced ure and

instructions. The antimicr obial susce ptibility pa ttern (S:

Susceptible, R: Resistant; and BLAC: β-lactamase positive)

antimicrobial concentr ation showing inhibitio n of growth

2.4. Biochemical Reaction Studies

using MicroScan Walk-Away

, system with PBPC 2 0 panel.

dehydration and rehydration wer e p erformed in a similar

way as mentioned in antimicrobial susceptibility assay for

2.5. Identification of Organism by Biotype Number

processed panel data report with the

help of biochemical reactions data [23, 24].

2.6. Amplification and Gene Sequencing of 16S rDNA

Genomic DNA was isolated from S. aureus cells (Gr. IIA,

sample coded as 9A) using genomic purification kit, according

to the manufacturer instructions. 16S rDNA gene (~1.5 kb)

polymerase chain reaction (PCR) using universal primers;

forward primer (5ˊ-AGAGTTTGATCCTGGCTCAG-3ˊ) and

reverse primer (3ˊ-ACGGTCATACCTTGTTACGACTT-5ˊ).

Amplified products were subjected to gel electrophoresis in

visualized under UV light in a gel documentation unit (BioRad

Laboratories, USA). The PCR amplified fragment was purified

Sequencing of amplified product was done on a commercial

basis from Bangalore Genei, India. The 16S rDNA sequences

obtained were aligned and compared with the sequences stored

in GenBank database available from National Center for

phylogenetic tree were established using MEGA3.1 molecular

3. Results and Discussion

3.1. Antimicrobial Susceptibility Test

The re sults of S. aureus susceptibility pattern and MIC

values of tested antimicrob ials after biofiel d treatment are

summarized in Table 1 and 2 respe ctively. T he data were

Antimicrobial se nsitivity assay and MIC were performed in

twenty-eight and thirty antimicrob ials respe ctively. T he

treated cells of S. aureus sho wed a significant (85.71% )

alteration (twenty- four out of t wenty-eight) in a ntimicrobial

sensitivity pa ttern from susceptible (S) to resistance (R) in

biofield t reatment a s compare d with control. However,

these twent y-four a ntimicrobials did not show any change

of sensitivity pattern on day 159 as well as in reviv ed state

twenty-eight antimicrob ials two antibiotics i.e. ampicillin

and penicillin were changed from S to β-lactamase positive

(BLAC) in lyophilized treated Gr. IIA on d ay 10 while

showed similar r esponse in res t of treated groups even af ter

second-time biofie ld treatment as compared with control. S.

penicillinase enzyme which br eakdown the β-lactam ring

present in p enems and c ephems heteronucleus [26]. Two,

out of twenty eight (7.14%) tested antimic robials such as

responses in lyop hilized treated cells (Gr. IIA) on day 10

while they exhibited susceptib le in rest of treate d samples

of S. aureus. Over all, 9 2.86% antimicrobial susceptibi lity

pattern was altered after bio field treat ment as compared to

cephalothin, chlo ramphenicol, cip rofloxacin, gati floxacin,

synercid showed an alter ation about two-fold in Gr. IIA on

day 10 as compared to control. Th e MIC value of

unchanged in rest of the groups as compar ed to control.

Certain antimicrobials such as erythromycin, oxacillin and

thirty two-fold (≤0.25 to >8 µg/mL) and penicillin showed

around two hundred sixty seven-fold (≤0.0 3 to >8 µg/mL)

alteration of MIC values in Gr. IIA on day 10 as compared to

sulfamethoxazole were slightly altered the MIC values in Gr.

IIA on day 10. Overall, 90% out of thirty tested

compared to control. Three out of thirty (10%) antimicrobials

did not show any alteration of MIC values in all the treated

groups as compared to control (Table 2) except piperacillin /

antimicrobial resistance pattern (S to R) and corresponding

MIC values were significantly alte red in lyophilized strain S.

aureus after first-time biofi eld treatment as compared to

3.2. Biochemical Reactions Studies

Data obtained from bioch emical reactio ns studies for

distinction o f S. aureus are illustrated in Table 3. Study of

enzymatic and metabolic chara cteristic feature of microbes.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

# data[].attributes.attachment_link.title
Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus - PdfSR.com

# data[].attributes.attachment_link.description
Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr.

# data[].attributes.headline
	
	
	
	
	 Biofield Impact on Genotyping Characterization of S. Aureus
	

# data[].attributes.subject
	
	
	
	
	 Biofield Impact on Genotyping Characterization of S. Aureus
	

# data[].attributes.body
Biofield treatment has the ability to alter the genotyping characterization of antimicrobials. The biofield can be an alternative approach than the existing antimicrobial therapy in near future.

# data[].attributes.attachment_link.title
"Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr.

# data[].attributes.headline
	
	
	
	
	 Investigation of Antimicrobial Susceptibility of Biofield Treated S. Aureus
	

# data[].attributes.subject
	
	
	
	
	 Investigation of Antimicrobial Susceptibility of Biofield Treated S. Aureus
	

# data[].attributes.body
Investigation of antimicrobial susceptibility of S. aureus was carried out with the help of automated instrument, MicroScan Walk-Away® to evaluate the impact of biofield treatment on S. aureus.

# data[].attributes.headline
	
	
	
	
	 Impact of Biofield Treatment on Staphylococcus Aureus’ Characteristics
	

# data[].attributes.subject
	
	
	
	
	 Impact of Biofield Treatment on Staphylococcus Aureus’ Characteristics
	

# data[].attributes.body
Read here the impact of biofield treatment on the characteristics feature of Staphylococcus aureus. The results of this study suggest that the biofield treatment can alter the characteristics of S. aureus.

# data[].attributes.attachment_link.title
Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus

# data[].attributes.attachment_link.description
Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on S. aureus. S. aureus (ATCC

# data[].attributes.headline
	
	
	
	
	 Biofield Treatment & Biochemical Reactions Pattern of S. Aureus
	

# data[].attributes.subject
	
	
	
	
	 Biofield Treatment & Biochemical Reactions Pattern of S. Aureus
	

# data[].attributes.body
The biochemical reactions pattern of S. aureus showed the significant 35.71% alteration when treated with biofield energy as compared to the untreated samples. Visit for more information.

# data[].attributes.headline
	
	
	
	
	 Genotyping Characterization of Biofield Treated Staphylococcus Aureus
	

# data[].attributes.subject
	
	
	
	
	 Genotyping Characterization of Biofield Treated Staphylococcus Aureus
	

# data[].attributes.body
This study shows the impact of biofield treatment on antibiogram, biochemical reactions and genotyping characterization of biofield treated Staphylococcus aureus. Read here for more details.

# data[].attributes.headline
	
	
	
	
	Biochemical Studies of Antibiogram of MDR Isolates of P. Aeruginosa
	

# data[].attributes.subject
	
	
	
	
	Biochemical Studies of Antibiogram of MDR Isolates of P. Aeruginosa
	

# data[].attributes.body
Biochemical study of antibiogram of MDR Isolates of P. aeruginosa showed a 48.48% change in tested biofield treated biochemical reactions out of 33 as compared to untreated samples.

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Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Road, Madhya Pradesh, India

Copyright:© 2015 Trivedi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,

distribution, and reproduction in any medium, provided the original author and source are credited.

In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been

noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi's

biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS

38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated.

Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration

(MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day

10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed 60% alteration in

sensitivity of tested antimicrobials in MDR isolates of P. aeruginosa after biofield treatment. MIC results showed an

alteration in 42.85% tested antimicrobials out of twenty eight after biofield treatment in five isolates of MDR P.

aeruginosa. Biochemical study showed a 48.48% change in tested biochemical reactions out of thirty three as

compared to control. A significant change in biotype numbers was reported in three clinical lab isolates of MDR P.

aeruginosa out of five, after biofield treatment as compared to respective control. On the basis of changed biotype

number (7302 0052) in biofield treated LS 23, new organism was identified as Citrobacter freundii as compared to

control (0206 3336). A very rare biotype number (7400 4263) was found in biofield treated LS 38, as compared to

control (0206 3736). Study results suggest that biofield treatment on lab isolates of MDR P. aeruginosa has

significant effect on the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number. Biofield

treatment might prevent the emergence of absolute resistance pattern of useful antimicrobials against MDR isolates

; Bioeld treatment; Multidrug-

resistant; Antimicrobial susceptibility; Biochemical reaction; Biotyping

Antimicrobial agents are widely used therapeutic option against

infections caused by pathogenic microbes. However, through dierent

strategies and mechanism, these microorganisms combat the eect of

antimicrobial agents, as multidrug resistant (MDR) clinical strains are

the best example world-wide.

is a ubiquitous, gram-negative bacterium and versatile opportunistic

pathogen, associated with nosocomial infections along w ith other

serious implications with high rate of morbidity and mortality [1].

Increasing resistant towards the available antimicrobials preclude the

eectiveness of any antimicrobial regimen [2,3]. B ecause of increasing

P.

isolates in health care setting, infections are

dicult to treat, causing life threating conditions [4].

one of the major pathogen related with hospital acquired infections

nosocomial infection surveillance system of center for disease control

is se cond most common cause of

nosocomial pneumonia, third most common in nosocomial urinary

bacteraemia [6]. MDR mechanism in

acquisition of resistance genes (β-lactamases) or b ecause of amino-

mutation involved against antimicrobials [8].

Despite of several advances in medical sciences, ne w generation

inhibiting the growth of bacterial cultures [10]. Bioeld is the name

surrounds living organisms. However, the energy can exists in several

forms such as kinetic, potential, electrical, magnetic, and nuclear.

chemical information in the form of electr ical signals. us, human

has the ability to harness the energy from environment or universe and

can transmit into any living or nonliving object(s) around the globe.

e objects always receive the energy and responding into useful way

[11,12], but very less controlled and experimental studies on bioeld

According to law of mass-energy inter-conversion [14], the conversion

of mass into energy is well stabilized, but its inversion

mass has not yet proved scientically. Whenever electrical signals

uctuate with time, the magnetic eld generates as per the Ampere-

Maxwell law, and cumulatively known as electromagnetic eld. Mr.

physicochemical and atomic characteristics of various materials. Mr.

Infectious Diseases & TherapyTrivedi et al., J Infect Dis Ther 2015, 3:5

http://dx.doi.org/10.4172/2332-0877.1000244

Research ArticleOpen Access

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 5 * 1000244

Trivedi's bioeld treatment had been studied and reported in altering

the antimicrobial susceptibility and biochemical reactions of microbes

against tested antimicrobials [15-17]. It has also signicantly reported

in eld of material science [18-20]. Bioeld treated crops had been

reported for a signicant change on growth, characteristics and yield of

plants [21-24]. Present study reports the impact of bioeld treatment

, for its antimicrobial susceptibility

biochemical reactions, and biotyping.

Experimental design and bioeld treatment

MDR clinical lab isolates (

LS 22, LS 23, LS 38, LS 47, and LS 58)

Microbiology Lab, Hinduja Hospital, Mumbai. Each MDR lab isolates

was divided into two groups

control and treatment. Treatment

group, in sealed pack were handed over to Mr. Trivedi for bioeld

treatment through his energy transmission process to the treated

were returned in the similar sealed condition for further analysis on

reactions, and biotype number were measured by MicroScan Walk-

Away® (Dade Behring Inc., USA) with respect to control group. All

antimicrobials and biochemicals were procured from Sigma-Aldrich,

USA.

Evaluation of antimicrobial susceptibility assay

P.

was studied using MicroScan Walk-Away® using Negative

Break Point Combo (NBPC 30) panel as per manufacturer's

instructions. e antimicrobial susceptibility pattern (S: Susceptible, I:

Intermediate, IB: Inducible β-lactamase; and R: Resistant) and MIC

concentration showing growth inhibition [25]. e antimicrobials used

in the susceptibility assay viz. amikacin, amoxicillin/K-clavulanate,

clavulanate, tobramycin, and trimethoprim/sulfamethoxazole.

determined by MicroScan Walk-Away® using NBPC 30 panel system in

both control and t reated groups [25]. Biochemicals used in the study

are acetamide, adonitol, arabinose, arginine, cetrimide, cephalothin,

citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose, hydrogen

penicillin, ranose, rhamnose, sorbitol, sucrose, t artrate, tryptophan

deaminase, tobramycin, urea, and Voges-Proskauer.

Identication by biotype number

e biotype number of MDR isolates of

treated sample were determined followed by identication of

microorganism by MicroScan Walk-Away® processed panel data report

with the help of biochemical reaction data [25].

Antimicrobial susceptibility pattern

e sensitivity pattern of MDR isolates of

pseudomonal antimicrobials agents are demonstrated in Table 1. All

Aztreonam (R → I in LS 22), cefepime (R → S in LS 23 and R → I in LS

47), imipenem (R → I in LS 58) and meropenem (R → S in LS 23 and R

→ I in LS 58) showed increased antimicrobial sensitivity pattern aer

antimicrobials also showed decrease in MIC values in treated group

ceriaxone antimicrobials changed from R → IB, while piperacillin

changed from IB → R and ticarcillin/K-clavulanate changed from IB →

I in LS 23. Antimicrobial sensitivity pattern of bioeld treated LS 38

piperacillin/tazobactam, and ticarcillin/K-clavulanate, and in case of

aztreonam and ceriaxone sensitivity changed from I → R, while S → R,

in case of cefepime. Bioeld treated LS 47 showed changed sensitivity

ticarcillin/K-clavulanate antimicrobials. Piperacillin showed changed

antimicrobial sensitivity pattern from R → IB in bioeld treated LS 58.

tobramycin antimicrobials did not show any change in sensitivity

pattern in bioeld treated clinical isolates of

AmikacinR RS S R R R R R R

CiprofloxacinR RR R R R R R R R

GentamicinR RR R R R R R R R

LevofloxacinR RR R R R R R R R

Piperacillin/tazobactamR R IB IB IB R R IB IB IB

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Antibiogram of Multidrug-Resistant Isolates of Pseudomonas

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 5 * 1000244

TobramycinR RR R R R R R R R

C: Control; T: Treatment; LS: Lab Isolate; R: Resistant; I: Intermediate; S:

Susceptible; IB: Inducible β-lactamases

multidrug resistant isolates of

P.

. Increasing antimicrobial resistance against various anti-

pseudomonal drugs in clinical isolates, has been reported world-wide

[26,27] which leads to a serious therapeutic problem. e resistance

showed varying degrees of resistance to

imipenem in recent years [28]. Obritsch

were well supported with literature data [29].

imipenem, and meropenem, antimicrobial sensitivity was improved

aerbioeld treatment in clinical MDR isolates.

Determination of minimum inhibitory concentration

MIC values of all the clinical MDR is olates of control and bioeld

are summarized in Table 2. O verall, 13 out of 28

tested antimicrobial (42.85%) reported with altered MIC values aer

bioeld energy treatment in clinical MDR isolates of

MIC values were decreased four fold in ceriaxone (less than equal to

8 µg/ml) and two fold in six antimicrobials

treatment in LS 23 isolate. Other isolates were also showed decreased

MIC values in tested antimicrobials such as aztreonam (16 µg/ml in LS

22), cefepime (16 µg/ml in LS 47), imipenem (8 µg/ml in LS 58),

meropenem (8 µg/ml in LS 58), nitrofurantoin (64 µg/ml in LS 23),

piperacillin/tazobactam (64 µg/ml in LS 47), and piperacillin (64 µg/ml

increased MIC values in treated LS 38. Piperacillin (in LS 23) and

ticarcillin/K-clavulante (in LS 47) also showed slight increase in MIC

values as compared to control (Table 2). Rest of antimicrobials did not

Amoxicillin/K-clavulanate>16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8

Ampicillin/sulbactam>16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8 >16/8

Ampicillin>16 >16 >16 >16 >16 >16 >16 >16 >16 >16

Cefazolin>16 >16 >16 >16 >16 >16 >16 >16 >16 >16

Cefotaxime>32 >32 >32 >32 >32 >32 >32 >32 >32 >32

Cefoxitin>16 >16 >16 >16 >16 >16 >16 >16 >16 >16

Cefuroxime>16 >16 >16 >16 >16 >16 >16 >16 >16 >16

Cephalothin>16 >16 >16 >16 >16 >16 >16 >16 >16 >16

Ciprofloxacin>2 >2 >2 >2 >2 >2 >2 >2 >2 >2

Gentamicin>8 >8 >8 >8 >8 >8 >8 >8 >8 >8

Levofloxacin>4 >4 >4 >4 >4 >4 >4 >4 >4 >4

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Antibiogram of Multidrug-Resistant Isolates of Pseudomonas

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 5 * 1000244

# data[].attributes.attachment_link.title
Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa.

# data[].attributes.headline
	
	
	
	
	Biofield Impact on Biochemical Characteristics of P. Aeruginosa
	

# data[].attributes.subject
	
	
	
	
	Biofield Impact on Biochemical Characteristics of P. Aeruginosa
	

# data[].attributes.body
Experimental results of biofield impact showed altered biochemical characteristics of P. aeruginosa. It positively affects the antimicrobial sensitivity, MIC values, biochemical reactions & biotype number of MDR Isolates of P. aeruginosa.

# data[].attributes.attachment_link.keywords[]
multidrug resistant isolates of pseudomonas aeruginosa

# data[].attributes.attachment_link.keywords[]
biochemical studies of mdr isolates of p. aeruginosa

# data[].attributes.attachment_link.title
"Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa.

# data[].attributes.headline
	
	
	
	
	Biofield Study of Pseudomonas Aeruginosa
	

# data[].attributes.subject
	
	
	
	
	Biofield Study of Pseudomonas Aeruginosa
	

# data[].attributes.body
Trivedi’s biofield treatment had been studied and reported in altering the antimicrobial susceptibility and biochemical reactions in clinical MDR lab isolates of P. aeruginosa.

# data[].attributes.headline
	
	
	
	
	Biofield & Biochemical Characteristics of Pseudomonas Aeruginosa
	

# data[].attributes.subject
	
	
	
	
	Biofield & Biochemical Characteristics of Pseudomonas Aeruginosa
	

# data[].attributes.body
Biofield treatment on lab isolates of MDR P. aeruginosa has significant effect on the biochemical characteristics of Pseudomonas Aeruginosa. Read here for more details.

# data[].attributes.attachment_link.title
Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment

# data[].attributes.attachment_link.description
In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on MDR

# data[].attributes.headline
	
	
	
	
	Biochemical Tests on Pseudomonas Aeruginosa
	

# data[].attributes.subject
	
	
	
	
	Biochemical Tests on Pseudomonas Aeruginosa
	

# data[].attributes.body
Biochemical tests or studies of MDR isolates of P. aeruginosa were determined by MicroScan Walk-Away® in both Biofield treated and untreated samples to identify the impact of Biofield effect.

# data[].attributes.headline
	
	
	
	
	Biofield Impact on MDR Isolates of Pseudomonas Aeruginosa 
	

# data[].attributes.subject
	
	
	
	
	Biofield Impact on MDR Isolates of Pseudomonas Aeruginosa 
	

# data[].attributes.body
The aim of this study is to determine the impact of Mr. Trivedi’s biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Follow the link for more information.

# data[].attributes.headline
	
	
	
	
	 Parameters for the Quality of Soybean Seeds and Soybean Productivity
	

# data[].attributes.subject
	
	
	
	
	 Parameters for the Quality of Soybean Seeds and Soybean Productivity
	

# data[].attributes.body
In a study the Soybean samples were successfully tested on different parameters of quality of soybean seeds and soybean productivity with Biofield treatment.

# data[].attributes.attachment_link.keywords[]
indole acetic acid content in cotton seeds

# data[].attributes.attachment_link.title
"Biofield and Fungicide Seed Treatment Influences on Soybean Productivity, Seed Quality and Weed Community" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Soybean production in Iowa USA is among the most productive for raínfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development.

# data[].attributes.headline
	
	
	
	
	Soybean Seed Treatment for Increasing the Productivity
	

# data[].attributes.subject
	
	
	
	
	Soybean Seed Treatment for Increasing the Productivity
	

# data[].attributes.body
Biofield Soybean Seed Treatment is a science that can be used as an alternative treatment in soybean production systems.

# data[].attributes.headline
	
	
	
	
	 Parameters for the quality of soybean seeds
	

# data[].attributes.subject
	
	
	
	
	 Parameters for the quality of soybean seeds
	

# data[].attributes.body
Biofield Treatment applied Soybean Seeds resulted improved results on various parameters for the Quality of Soybean Seeds. Visit the links here for more details.

# data[].attributes.attachment_link.title
Biofield and Fungicide Seed Treatment Influences on Soybean Productivity, Seed Quality and Weed Community

# data[].attributes.attachment_link.description
Soybean production in Iowa USA is among the most productive for rainfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate

# data[].attributes.headline
	
	
	
	
	 Nutrient Value of Soybean, Soybean Fungicide Seed Treatment
	

# data[].attributes.subject
	
	
	
	
	 Nutrient Value of Soybean, Soybean Fungicide Seed Treatment
	

# data[].attributes.body
The application of Biofield Treatment on Soybean resulted better nutrient value of Soybean and improved soybean productivity. Follow the link for more information.

# data[].attributes.headline
	
	
	
	
	 Biofield & Fungicide Seed Treatment
	

# data[].attributes.subject
	
	
	
	
	 Biofield & Fungicide Seed Treatment
	

# data[].attributes.body
Biofield & Fungicide Seed Treatment was applied on Soybean seeds to determine the inﬂuences of Soybean productivity, seed quality and Weed Community. Visit for more details.

# data[].attributes.headline
	
	
	
	
	



	
	
	
	
	Soybean Seed Treatment & Soybean Productivity
	





	

# data[].attributes.subject
	
	
	
	
	



	
	
	
	
	Soybean Seed Treatment & Soybean Productivity
	





	

# data[].attributes.body
Soybean seed treatment using Biofield energy resulted better soybean productivity in a recent research. Read here a comparative study.

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Biofield and Fungicide Seed Treatment Influences on Soybean Productivity,Seed Quality and Weed Community

Soybean production in Iowa USA is among the most productive for rainfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development. A field study was conducted at two locations in Iowa in 2012 to determine if seed-applied fungicide or biofield treatments influenced weed community, soil volumetric water concentration and soybean yield and quality. Application of biofield treatment resulted in lower density of tall waterhemp density, greater soybean stand density at R8 stage and greater seed pod-1 compared to the absence of seed fungicide and biofield. Soil volumetric water content varied by seed fungicide x biofield x date interaction but differences were not consistent among treatment combinations. Overall, seed fungicide and biofield treatments had similar effects on soybean productivity, however additional research is necessary to determine if biofield treatment is a suitable replacement for seed fungicide application.

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Biofield and Fungicide Seed Treatment Influences on Soybean Productivity,Seed Quality and Weed Community - PdfSR.com

# data[].attributes.attachment_link.description
Soybean production in Iowa USA is among the most productive for rainfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development.

# data[].attributes.headline
	
	
	
	
	Increasing Chlorophyll Content in Tomato Plant
	

# data[].attributes.subject
	
	
	
	
	Increasing Chlorophyll Content in Tomato Plant
	

# data[].attributes.body
Biofield treatment was applied on tomato plant to study the effects on its characteristics. The energy treatment resulted increased chlorophyll content.

# data[].attributes.attachment_link.title
"Impact of Biofield Treatment on Growth and Yield of Lettuce and Tomato" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var.

# data[].attributes.headline
	
	
	
	
	
		 Spad Value Chlorophyll Content, Chlorophyll Content Meter
	

# data[].attributes.subject
	
	
	
	
	
		 Spad Value Chlorophyll Content, Chlorophyll Content Meter
	

# data[].attributes.body
Read here the effect of biofield treatment on tomato and lettuce. The Spad Value chlorophyll content meter was used to measure the chlorophyll content of samples during the analysis.

# data[].attributes.attachment_link.title
Impact of Biofield Treatment on Growth and Yield of Lettuce and Tomato

# data[].attributes.attachment_link.description
Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied

# data[].attributes.headline
	
	
	
	
	Chlorophyll Content Meter, Spad Value Chlorophyll Content
	

# data[].attributes.subject
	
	
	
	
	Chlorophyll Content Meter, Spad Value Chlorophyll Content
	

# data[].attributes.body
Chlorophyll content when measured using a Spad Value chlorophyll content meter in biofield treated samples of tomato and lettuce samples, shows better results compared to the untreated samples.

# data[].attributes.headline
	
	
	
	
	Increasing Tomato Yield, Chlorophyll Content in Tomato Plant
	

# data[].attributes.subject
	
	
	
	
	Increasing Tomato Yield, Chlorophyll Content in Tomato Plant
	

# data[].attributes.body
A recent study suggest that the biofield treatment can be applied for increasing tomato yield and also the quality of tomato. Higher leaf tissue chlorophyll content was measured in treated tomato and lettuce plants.

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Australian Journal of Basic and Applied Sciences, 6(10): 100-105, 2012

Corresponding Author: Shrikant Patil, Trivedi Foundation, Scottsdale, AZ, USA.

Impact of Biofield Treatment on Growth and Yieldof Lettuce and Tomato

1Vishal Shinde, 1Frank Sances, 2Shrikant Patil and 1Amy Spence

1Pacific Ag Research, San Luis Obispo, CA, USA.

Abstract: Recent studies report the effect of biofield treatment on changes in structural characteristics

of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study

tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca

sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without

fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with

fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal

wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants

with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that

received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied

treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated

tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The

combination of biofield treatment along with administration of chemical additives demonstrated the

best results with statistically increased yields and higher pest resistance in both test cropping systems.

The specific mechanisms that lead to these preliminary results have yet to be determined.

Key words: Crop development;Biofield treatment; Fertilizer and Organic; Lettuce; Tomato.

Information-containing biofield energies have been postulated to be associated with living organisms and to

affect their self-regulation processes (Rubik, 2002). Recent studies by Trivedi and Tallapragada (2008, 2009)

present enhanced and lasting transformations seen in the physical and structural properties of organic and

inorganic materials which were the effect of consciousness energy when transmitted using specific techniques.

These authors report that elemental diamond, graphite and activated charcoal powders showed measureable and

significant changes in their molecular structure, and go on to suggest that thebiofield energy may involve

electromagnetic and weak interactions.Dabhadeet al. (2009) suggest thatmeasurable changes in particle size and

hence surface area as well as crystallite size of two test substances, antimony and bismuth metal powders,which

they observed as a consequence of a similar treatment,was due to increased energy states caused within the

treated substances.Trivedi and Patil (2011) reported multiple year results on Alphonso Mangos in Asia. The

mango study showed yield increases and pest infestation decreases over a four year time period versus control

trees which on the contrary showed lower yields and increased pest pressure. More recently the biofield energy

was tested on Patchouli micropropagation (Patilet al., 2012) where it was reported to increase regeneration and

cause an overall improvement in plant health. In related studies, the biofield energy was reported to impact

cancer cells in vitro (Yount et al., 2012).The studies on living organisms are able to probe more relevant aspects

of the information content in biofield energies, in order to establish both the reality of the impact as well as its

nature. However, both the in vitro studies were in protected environments showing variability in the results. It

can be expected that adaptive forces would bebetter testedin vivo in the field where the normal challenge of the

environment is experienced and the informational integrity of the organism is also maintained. The nature of

thebiofield energy in these experiments may be expected to produce more well-defined results under such

conditions. However, the mango study was performed on a pre-existing pest infested mango orchard and does

not document the results of systematic treatment under a variety of conditions.

Although science has earlier tested such energies in plants, in the above-mentioned studies the scientific

facts to support such claimsare for the first time seeingreproducible and significant results in experimental

observations. The source of biofield energy treatments are those of an internationally reputed energy healer

whose name is here withheld according to recommended best scientific practice, but can be provided on demand

for replication experiments. While any improvement in human health under such interaction can be accounted

for by the placebo effect, such results in plant systems indicate some basic properties of living organisms which

need to be noted. An understanding of the energy and its use can help improve the environmental conditions

during plant cultivation.In this paper we report on the observed results of crop quality and yield of treated and

untreated tomato and lettuce plantings in organic conditions and in the presence of needbase fertilizers and

pesticides applied according to standard procedure. Scientific mechanismsare not here speculated upon; at this

Aust. J. Basic & Appl. Sci., 6(10): 100-105, 2012

stage we report the positive results observed in our experiments. Further experimentation is necessary in order to

generate the hypotheses regarding underlying mechanisms.

The objective was to conduct a blind study to determine the potential influence of biofield therapy on

growth & development of lettuce & tomato in commercial plantings and to systematically compare the effects

with and without application of fertilizers and pesticides.The treated and untreated plants were planted in plots

in randomized fashion and the location of treated plants remained undisclosed to evaluators during the study.

Seeds were treated and allowed to germinate until ready to be transplanted. The seedlings were treated

again, transplanted into an open field, and allowed to develop according to the season. As a control, untreated

seeds were allowed to germinate in the same manner and transplanted alongside the treated plots in a

randomized fashion. Needbase fertilizers and pesticides were applied onto one set of plots with transplants from

both untreated and treated seeds. Another set of plots for both untreated and treated transplants did not receive

The Biofield treatment was applied for about 3 minutes from a distance of about 1 meter from the samples.

The energy source individual was escorted to the laboratory for treating seeds and to the field for tre ating

transplants, was seated on a chair at the given distance from the material to be treated, and was observed to

focus concentrated thought outwardly towards the seeds or plants ready to be transplanted. The untreated

samples were not in the same room or plot at the time.Each treated set received the treatment twice, the seeds

once before being germinated and the resulting plants 53 days later just before they were transplanted in the

Treated and untreated lettuce plants were planted in separate randomly allocated plots in five replicates

measuring 3.33' by 60' on clay loam soil.Treated and untreated tomato plants were similarly planted in separate

plots measuring 6.66' by 50' on sandy loam soil.Plots were hand transplanted, hand harvested, and drip

Needbase fertilizer and pesticide applications:

Fertilizer 18:6:12 was applied at a rate of 600 lb/a slow release pre-planting.The following pesticides were

applied 18 days after transplanting (DAT): (a) Endura at a rate of 11 oz/a for the purpose of controlling

controlling Downy Mildew (Bremialactucae ). (c) Maneb at a rate of 2 lb/a for the purpose of controlling Downy

Fertilizer 18:6:12 was applied at a rate of 600 lb/a slow release pre-planting.Fertilizer 7:7:7 was applied at

45, 52, 59, 66, and 80 DAT at a rate of 20 gal/a. The following pesticides were applied: (a) Bravo Weatherstik at

a rate of 1.5 pt/a for the purpose of controlling Late Blight (Phytophthorainfestans ) on 63, 84, and 90 DAT. (b)

Quadris at a rate of 10.5 oz/a for the purpose of controlling Powdery Mildew (Oidiumneolycopersici ) on 63, 84,

and 90 DAT. (c) Ran-man at a rate of 3.0 floz/a for the purpose of controlling Late Blight

(Phytophthorainfestans ) on 114 and 121 DAT.

All evaluations were carried out in blinded fashion, with assessors unaware of the location of treated plants.

at 21, 35, 50, and 56 DAT. Stand reduction was calculated using the formula:

Percent reduction in stand=100 x (Reduced Stand / Original Stand)

Chlorophyll content was measured using a spad meter (Spectrum Technologies, Inc., Plainfield, IL) from 10

plants per replicate plot at 32 DAT.Lettuce heads were harvested 56 days after transplanting, and were assessed

for marketable quality and size based on market standards.

Aust. J. Basic & Appl. Sci., 6(10): 100-105, 2012

Crop vigor and stand counts were assessed at 41 DAT and plants measured for height and diameter.

Chlorophyll content wasmeasured at 89 DAT, just before start of harvesting, using a spad meter,from 10 plants

per replicate plot. Tomatoes were harvested over the course of seven pickings between104 t o 126 days after

transplantingand were assessed for marketable quality and size based on market standards.Roots from ten

tomato plants were analyzed for severity of Nematode infection based on a 0-10 scale with 10 representing

maximum severity and lycopene content was assessed at 129 DAT.

Wilted plants and stand reduction results by treatment are presented in Table 1. Biofield treated seeds with

fertilizers/pesticides (TF) showed the lowest amount of total wilted plants per replicate plot.This resulted in the

lowest stand reduction, 14.2%, in comparison to control seeds with fertilizers/pesticides (CF) at 27%.Although

both control and biofieldtreated seeds without fertilizers/pesticides (designated C and T, respectively) showed

similar wilted plants to the CF, the percent loss of stands in T plots were statistically similar to CF plots,

suggesting that the externally applied energy created healthier and more vigorous plants, abl e to withstand

unaltered field conditions. Figure 1 shows the differences in chlorophyll content between treatments. TF and T

plots were observed to have statistically higher chlorophyll content than CF and C treatments.Chlorophyll

content is largely determined by plant nutrition, health status, and exposure to sunlight (i.e. conversion of

protochlorophyllide to chlorophyll); however, all plants in the study received the same amount of sun exposure,

suggesting that the energy treatment had changed certain physiological processes which are beyond the scope of

this study. TF plots were observed to have the greatest number and weight of marketable lettuce heads than any

other treatment, and a greater amount of large (18s) and medium (24s) heads were harvested from these plots as

well (Table 2). TF and CF received the same amounts and types of fertilizers and pesticides, yet yield and

quality was approximately 43% greater in the TF plots. Further, Figure 2 shows that the TF treatment resulted in

a statistically higher total of percent marketable heads compared to all other treatments. If the energy transmitted

is generating change in metabolic processes to the plants, this may explain why TF plots exceeded yield and

quality amounts of T plots, enabling a greater intake of fertilizer nutrients and thereby creating stronger and

Table 1: The number of wilted plants and total stand reduction of control and treated lettuce plants. Means followed by the same letter do

not differ. (Fisher's LSD test).

Wilted Plants (#) Stand Reduction

Control with no fertilizers or pesticides

Control with needbase fertilizers and

5.00 a 6.60 a 12.00 b 6.00 a 29.60 a 26.73 b

Treated with no fertilizers or pesticides

5.40 a 9.80 a 15.20 ab 1.40 b 31.80 a 29.07 b

Treated with needbase fertilizers and

3.80 a 3.20 a 5.40 c 0.80 b13.20 b 14.22 c

Table 2: The number (a) and weight in kg (b) of marketable lettuce heads harvested. Means followed by the same letter do not differ.

(a) Marketable Lettuce Heads #

(b) Marketable Lettuce Heads (kg)

# data[].attributes.attachment_link.title
Impact of Biofield Treatment on Growth and Yieldof Lettuce and Tomato - PdfSR.com

# data[].attributes.attachment_link.description
Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var.

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	 Poultry Farming Project - The Trivedi Effect
	

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	 Poultry Farming Project - The Trivedi Effect
	

# data[].attributes.body
The poultry farming project was aimed to improve the Health, Weight and Food intake of chicken with the help of biofield energy. The energy treatment could be used in poultry farm business plan for poultry raising.

# data[].attributes.headline
	
	
	
	
	Dairy Farming Project - The Trivedi Effect
	

# data[].attributes.subject
	
	
	
	
	Dairy Farming Project - The Trivedi Effect
	

# data[].attributes.body
The dairy farming project is a study of The Trivedi Effect on quality of Milk. Read here to know about the project report about how energy treatment could change the dairy farming in India.

# data[].attributes.headline
	
	
	
	
	Biofield Impact of Bottle Gourd Plant & Crop
	

# data[].attributes.subject
	
	
	
	
	Biofield Impact of Bottle Gourd Plant & Crop
	

# data[].attributes.body
The alternative energy treatment by Mr. Mahendra Trivedi has resulted significant improvement in quality of bottle gourd plant. So the energy treatment could be used for better bottle gourd crop.

# data[].attributes.headline
	
	
	
	
	Mango Fruit Production - Impact of The Trivedi Effect
	

# data[].attributes.subject
	
	
	
	
	Mango Fruit Production - Impact of The Trivedi Effect
	

# data[].attributes.body
The study was intended to use The Trivedi Effect for increasing the mango fruit production. In this Mango Project the alphanso mange trees were used for study and results evaluated. Visit for more details.

# data[].attributes.headline
	
	
	
	
	 Cashew Project - The Trivedi Effect Impact on Cashew Nut Trees
	

# data[].attributes.subject
	
	
	
	
	 Cashew Project - The Trivedi Effect Impact on Cashew Nut Trees
	

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Mr. Mahendra Trivedi's biofield Energy treatment was studied on the cashew nut trees to evaluate the impact of his energy on cashew nut cultivation. Read for more details.

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	 Mahendra Trivedi's Biofield Energy | The Trivedi Effect
	

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	 Mahendra Trivedi's Biofield Energy | The Trivedi Effect
	

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Mahendra Kumar Trivedi has conducted thousands of experiments based on the science of Biofield Energy and The Trivedi Effect. Visit to know more about the science behind The Trivedi Effect.

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The Science Behind The Trivedi Effect®

Atoms exist in all states of matter. Even the cells of microbes, plants, human beings and all other living / non-living things consist entirely of atoms in the form of complex molecules. Atoms, at the most elementary level, are made from electrons, protons, neutrons and several known and unknown subatomic particles. Physicists and chemists hypothesize several theories to explain the systematic and disciplinary existence of various species inside the atom, the nature of bonds between atoms in condensed states (such as solids and liquids), as well as for the way the particles are working inside the atom.

There have been several attempts to develop a unified picture that links the macroscopic universe and the microscopic atomic and subatomic world. The electromagnetic spectrum, of which visible light only forms a small portion of, arises from the cosmic radiation filtered by the atmosphere surrounding the planet. The observed properties of the matter and the electromagnetic radiation could only be explained by assuming a dual nature. That is, we view the object as a particle and use words to describe the particle's mass, speed and energy. On the other hand, we view the object as a wave and use words like wavelength and frequency to describe that wave. With this picture in mind, the radiation waves propagate through several tiny bundles of energy called photons and exert electrical and magnetic fields in mutually perpendicular directions. The energy varies inversely with the wavelength of the radiation and is high for gamma rays and low for radio waves with several regions of intermediate energy in between. Depending on the energy of radiation, the effects of interaction with matter are different. While gamma rays affect the nucleus of the atom, ultraviolet rays affect the outer electrons, and the infrared waves and microwaves affect the molecular vibrations and rotations. Therefore, the various regions of electromagnetic radiation are used to fingerprint different levels of structure at the atomic and molecular level.

The dual nature of matter and radiation led to quantum indeterminacy. The apparent necessary incompleteness in the description of a physical system has become one of the characteristics of the standard description of quantum physics. Prior to quantum physics, it was thought that (a) a state of a physical system can be uniquely determined by all the values of its measurable properties, and conversely (b) the values of its measurable properties uniquely determined the state. This made late Albert Einstein skeptical of quantum indeterminacy and in 1926, in a letter to Max Born, Einstein wrote: "I, at any rate, am convinced that He [God] does not throw dice." This became one of his greatest and most philosophical statements.

Atoms and subatomic particles are in continuous movement. Einstein postulated that neither the time, the weight, nor the mass are constant. When moving at high speeds, all of these things get compressed; only the speed of light remains the same. Einstein says this happens because energy is equal to mass times the speed of light squared, or E=MC2. This led to the concept of the atomic bomb, where the mass of the tiny atom is converted into tremendous energy. The reverse concept of converting energy into mass has yet to be observed.

Gravity is yet another energy that plays a dominant role at the macroscopic level (at the level of large masses like human beings, planets etc.). Einstein attempted to generalize his theory of gravitation in order to find a single unifying force, the special cases of which are gravitation and electromagnetism.

According to the prophetic novel "The Trigger," written by late Sir Arthur C. Clarke and Michael Kube-McDowell, the well-known CERN model does not say anything about why matter exists. Rather, the understanding is energy is energy, and matter is also energy. Forces are transmitted by vector bosons, which are elementary particles, and confusion arises because particles are matter, and matter is energy. The authors pose the question, "What is the factor which collapses the primal energy field into so many discrete fields states - four forces, a dozen of elementary particles, a hundred elements, a hundred thousand compounds? What is the essence of differentiation that allows a drawer full of pencils, a sky full of snowflakes, a beach full of sand grains to exist - with each element of the set retaining its individual character and separate existence, without sacrificing the underlying identity of form?" Their hypothesis was "Information organizes and differentiates energy. It regularizes and stabilizes matter. Information propagates through matter-energy and mediates the interactions of matter-energy. It is the mind of creation, the anti dyne to chaos."

The matter is considered to be a mere derivation: a subordinate phenomenon. The fundamental essences are energy and information. Information binds energy into form as will binds volition into purpose. "Alter the information, and the form is altered, while the substance remains unchanged." According to them, the information was the Universe's Will, imposing order on the Universe's substance-the order that had followed in the wake of the spontaneous and explosive transformation wrongly taken as the moment of creation. Energy is older than information, but formless and timeless without it: older than matter, but helpless and useless without it. In this new and provocative view, the Big Bang was not the birth of the Universe, but the birth of its consciousness.

Life Energy/consciousness/information signals the universe exists and needs a medium through which it can express itself. This has to be an entity different from all living beings: a unique individual on this planet.

Mr. Mahendra Kumar Trivedi (lovingly known as "GURUJI") is the one who defies the laws of human physiology and has performed what a layman would call "miracles." Although his physiology is totally different in comparison to that observed in normal human beings, he possesses excellent health with a fully functioning body and brain and very high levels of perception, interpretation and execution of daily activities. His food intake and his sleep are as normal as any human.

Mahendra Trivedi has the ability to harness the Divine Force/Vital Force from the Universe and to transmit/infuse it into any living or nonliving substance located anywhere on this planet. This is called The Trivedi Effect®. The energy of The Trivedi Effect® can make permanent changes into the structure of the atom, including the distance between two atoms, the size of the atom, the mass of the atom and the energy within and between the atoms, which are the basic building blocks that compose the entire known universe, visible and nonvisible. Mr. Trivedi's energy/consciousness/information signals, otherwise referred to as "The Trivedi Effect®," have altered the gene sequence by huge proportions in all varieties of plants and microbes, as well as changed interactions within and between atoms. Normally, the changes caused by external applied force/energy will revert back due to the restoring forces operating at the atomic level. However, the above-mentioned changes are found to be permanent and do not alter even after the force is removed (even in the absence of Mr. Trivedi's physical presence).

It may also be noted that, to alter the gene sequence, it is necessary for the technology in the form of energy/force to remain there permanently. Only then can new gene sequences express themselves in the DNA as well as form new proteins and provide unusual results (which are not seen in traditional varieties of crops and microbes). DNA fingerprinting is a rather crude and elementary method to analyze the impact of The Trivedi Effect®, as in any DNA even a single gene sequence consists of molecules with a large number of atoms. In such a system, the molecules are bound by weak secondary forces, which are somewhat easier to manipulate. Hence, to understand this impact/phenomena of The Trivedi Effect®, it is essential to conduct investigations at the level of atoms where the energy within and between the atoms is caused by strong primary forces.

Mahendra Kumar Trivedi has conducted thousands of experiments on metals, ceramics, polymers, chemicals, organic and pharmaceutical compounds through XRD, Mass Spectroscopy, Thermal analysis, Particle Size Analyzer, Surface area, ESR etc. Based on the results of these experiments, it was concluded the biofield treatment of The Trivedi Effect® can alter the distance between the atoms in a unit cell; change the energy in the crystalline structure as well as the crystallite size and the energy quantum between the atoms and within the atom. As per the existing science, if by some means one can alter the above parameters then the behavior of the atoms (and resultantly the materials) in both living and non-living things can be changed. Further, this energy constantly controls/regulates all of the constituents within the atom and between the atoms in such a manner that the atomic behavior and the crystalline behavior of the material must be altered throughout and remain the same all the time, anywhere on this planet.

Based on the results obtained and documented, it can be concluded that Mahendra Trivedi can induce the energy permanently into the atomic world through the procedure of thought intention. The same experiments conducted through the same machines on the same materials (both living and non-living) miraculously yielded different results in terms of quantity and quality when different amounts of energy/information/programming were fed. Every time, we have obtained different behaviors between the Control and Treated samples and also between different Treated samples too, which did not match with each other, by inducing different information every time in terms of the biofield treatment of The Trivedi Effect®. As Sir Arthur C. Clarke and Michael Kube-McDowell pointed out, "Alter the information, and the form is altered, while the substance remains unchanged."

The results of experiments on microbes showed that The Trivedi Effect® changed the species of microbes (based on the data through 16s rDNA). Hence the biofield treatment was able to create a new species of microbes or was able to "rewrite the destiny" of the microbes to be different in the 2nd, 3rd, 4th and so on generations. Thus, his energy has the capacity of creation, which was already shown through the experiments using mass spectroscopy and XRD for non-living materials, where energy was converted into mass. As per Mr. Trivedi, there is only ONE energy. Based on this fact, the same energy creates different types of impacts with varying results on both different living and non-living things.

Therefore, there is little surprise that Mahendra Trivedi can guide, control and regulate the energy through his thought intention procedure, which in turn significantly alters the behavior of the individual atom. It also alters groups of atoms at the level of the crystalline and poly crystalline states in inorganics and large molecular structures in organics, polymers and living things. In doing so, sometimes his physical presence is not required. Many times, he has treated living/non-living things from different corners/countries on this planet.

Also, there is no method according to the existing science (of any discipline) where energy can be converted into mass. Rather, only mass has been converted into energy. Through various experiments, The Trivedi Effect® has shown energy convert into mass (within the earth's gravitational force), thereby proving Einstein's concept that E=MC2 to be absolutely applicable in the case of this miraculous energy.

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The Trivedi Effect, Mahendra Trivedi | Trivedi Science

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Know the science behind The Trivedi Effect® and bio field energy. Mahendra Trivedi harness the Divine Force/Vital Force from the Universe and to transmit/infuse it into any living or nonliving substance located anywhere on this planet. This is called The Trivedi Effect®.

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		About Mahendra Trivedi | The Trivedi Effect
	

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		About Mahendra Trivedi | The Trivedi Effect
	

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Mr. Mahendra Trivedi has the ability transform living organisms and non-living materials through the transmission of a specific, healing energy. This phenomenon is known as The Trivedi Effect. Read here for more details.

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The unique physiology of Mahendra Trivedi has been studied and documented extensively in the USA, Australia, Canada and India by renowned clinical specialists and scientists. The reports are mind-boggling indeed and defy imagination. However, they do prove one simple fact -
Mr. Trivedi has a physiology that is significantly different from all the other seven billion human beings on Earth.

Some of the unique aspects of Mr. Trivedi's physiology include:

The transmission speed in Mr. Trivedi's blood vessels was beyond the maximum limit designated by technology

The arteries showed zero percent atherosclerotic plaque at 41 years of age

At the age of 54, the X-Ray and MRI findings of his spine appeared similar to those of a healthy male who had completed growth plate fusion and was no older than 25 years of age

Full body, digital X-Rays show that he has growing cartilage at the age of 54 years!

The muscle density in his hips is either higher or similar to bone density because his muscles are visible in X-Ray examination at 51 years of age

The elasticity of his body's cells at 41 years of age is much more superior than that of any newborn baby on this planet

The lowest body temperature is 68 degrees Fahrenheit and the highest body temperature is 95 degrees Fahrenheit only

The size of the pituitary fossa is 18.5 mm by 12.8 mm which is the largest ever noted in a healthy subject

The breathing is abdominal with little or no movement of the diaphragm which is biomechanically impossible

An EEG found that his brain is in an alpha state when he is simply "normal"

The sympathetic and parasympathetic parts of his brain function simultaneously, which is biomechanically impossible for humans because both systems produce an opposite impact in blood flow; muscle function; hormone, enzyme and peptide release, etc.

According to Dr Brian Costello, Chairman, American Board of Psychological Specialties in Neuropsychology, "Mr. Trivedi's computerized biofeedback measurements were found to be "uniquely dissimilar" from any other patient and non-patient samples. Mr. Trivedi's brain is into the deepest state of calmness and at the same time it is in the highest state of excitement, which is impossible with a normal human brain"

With Mr. Trivedi's physiological parameters, no other human can survive for even a small period of time.

Clinicians believe that there may be a link between his unique physiology and his extraordinary ability to transform the living organism at cellular level and non-living materials at atomic level.
To know more about him, Please Click Here

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Mahendra Kumar Trivedi | The Trivedi Effect

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The unique physiology of Mahendra Trivedi has been studied and documented extensively in the USA, Australia, Canada and India by renowned clinical specialists and scientists. The reports are mind-boggling indeed and defy imagination. However, they do prove one simple fact - Mr. Trivedi has a physiology that is significantly different from all the other seven billion human beings on Earth.