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Members:
1. Hong Siew Yi
2. Nur Nadhrah
3. Nurul Sakinah
4. Ho Pei Yi
5. Mashitoh

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Members:
1. Kok Liang
2. Angelica
3. Wei Yi
4. Azlia
5. Suhail

Chemolithotrophy

1) For what two purposes are inorganic compounds used by chemolithotrophs?

As an energy source and electron donor

2) Why are the sulphur-oxidizing lithotrophs typically acidophiles?

Because they oxidize reduced sulphur compounds producing sulphuric acid, which causes their surroundings to become acidic. Thus, making this microbe capable of adapting to extremely acidic environments.

3) What is the function of rusticyanin and where is it found in the cell?

Rusticyanin is a blue copper protein and it is obligate acidophile chemolithoautotroph (Acidithiobacillus ferrooxidans).  Its function is to act as an electron transfer protein.

Can be found in the acidophilic sulfur bacterium Thiobacillus ferrooxidans.

4) What is the inorganic electron donor for Nitrosomonas? For Nitrobacter?

Nitrosomonas: Ammonia (NH4+) to nitrite (NO2-)

Nitrobacter: Nitrite (No2-) to nitrate (NO32-)

5) What do nitrifying bacteria use as a carbon source? CO2

Use carbon dioxide as a carbon source

6) What is the anammox reaction and how does it differ from aerobic nitrification?

Anammox reaction is a reaction which will oxidize the ammonium into dinitrogen gas by using nitrite (electron acceptor).

Anammox is anaerobic ammonium oxidation which will oxidize the ammonium into dinitrogen gas while aerobic nitrification will undergo oxidation, oxidized ammonia into nitrite.

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Members:
1)Yong Zi
2)Adlina
3)Kai Xuan
4)Anis Zaid

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YEOH SHIR LING A18SC0495

ANIS SYAKIRA MOHAMAD A18SC0379
YAP YING YAN A18SC0493

STEVEN ANAK LILOI A18SC0483

NORARINA SURAYA MOHD RAZALI A18SC0431

1. What is the difference between oxygenic and anoxygenic phototroph?

The key difference between oxygenic and anoxygenic photosynthesis is that

Oxygenic phototroph

generates molecular oxygen during the synthesis of sugar from carbon dioxide and water

Oxygenic phototrophy is used by cyanobacteria containing chlorophyll a, with two distinct photosystems, each containing separate reaction centers.

allows for the generation of both ATP and reducing power in one process, facilitating photoautotrophic growth through the fixation of CO2.

Examples: cyanobacteria, algae, and green plants.

Anoxygenic phototroph

does not generate oxygen.

have a single photosystem with bacteriochlorophyll, allowing them to use cyclic photophosphorylation as described above for the formation of ATP.

Allow bacteria grow in anaerobic conditions

for most groups of anoxygenic phototrophs, the presence of oxygen hinders the formation and functioning of their photosynthetic machinery and pigments

Examples: purple bacteria, green sulfur and nonsulfur bacteria, heliobacteria, and acidobacteria.

2. How are photoautotrophs and photoheterotrophs similar and how do they differ?

Similarities

organisms that use light as their source of energy to produce ATP and carry out various cellular processes

Differences

Photoautotrophs convert inorganic materials into organic materials for use in cellular functions such as biosynthesis and respiration and provide nutrition for many other forms of life.

Photoheterotrophs depend on light for their source of energy and mostly organic compounds from the environment for their source of carbon.

3. Group the photosynthetic bacteria according to its trophy and oxygenic/anoxygenic phototrophs

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Members

1. Hannah Nadiah

2. Norazrin Anis

3. Tan Jia Hao

4. Pua Lei Wen

5. Lee Xin Yi

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Member:
1. Bryan
2. Li teng
3. Farrah
4. Talitha
5. Isyafatira

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Member
1. Nur Aisyah
2. Zi Ning
3. See Ting
4. Fatin Nabilah
5. Patrick

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1. Kok Liang
2. Angelica
3. Wei Yi
4. Azlia
5. Suhail

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Members:
1. Hong Siew Yi
2. Nur Nadhrah
3. Nurul Sakinah
4. Ho Pei Yi
5. Mashitoh

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Members:
1. Jane Seng
2. Al-Fayyadh
3. Anis Nadia
4. Jing Wen
5. Nur Ayunie

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1. Tan Jiayong
2.imran
3.Kek jazz yee
4.Ameera
5.Syuhaidah

1)What is the difference between oxygenic and anoxygenic phototroph?

Oxygenic

Converts light energy to chemical energy by certain photoautotrophs by generating molecular oxygen.

Organisms such as cyanobacteria, algae, and green plants

Involve 2 photosystems

Electrons travel in a noncyclic chain

Water is used as the initial electron

NADPH is generated donor.

Anoxygenic

Converts light energy to chemical energy by certain bacteria without generating molecular oxygen.

Organisms such as purple bacteria, green sulfur and nonsulfur bacteria, heliobacteria, and acidobacteria.

Involve only 1 photosystem

Electrons are transported in a cyclic chain

Water is not used as an electron donor

NADPH is not generated

2) How are photoautotrophs and photoheterotrophs similar and how do they differ?

Similarities

Use light as their energy sources to produce ATP and carry out their cellular processes

Difference
Photoautotrophs

Use inorganic compounds (carbon dioxide) as their carbon sources

Can synthesize their own food by photosynthesis

Convert carbon dioxide and water into organic materials for cellular functions (biosynthesis, respiration)

Eg: Oxygenic photosynthesis: Plants, algae, cyanobacteria
Anoxygenic photosynthesis: Green and purple bacteria

Photoheterotrophs

Use organic compounds (sugars, carbohydrates) as their carbon sources

Cannot synthesize their own food

Obtain organic carbon sources from autotrophs and environment as their food

Eg: Green nonsulfur bacteria, Purple nonsulfur bacteria

3) Group the photosynthetic bacteria according to its trophy and oxygenic/anoxygenic phototrophs

Oxygenic bacteria - cyanobacteria photoautotrophic

Anoxygenic - green sulfur bacteria, purple sulfur bacteria ,filamentous anoxygenic photorophys, photorophic acidobacteria, and phototrophic heliobacteria

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Activity-trophy levels
Members:
1)Anis
2)Adlina
3)Kai Xuan
4)Yong Zi

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Members:
1. Asyura
2. Hui Nee
3. Lok Yee
4. Taib
5. Zen Dong

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Members:
1. Bryan
2. Li teng
3. Isya
4. Farrah
5. Talitha

Topic: Streptomycin

1. secondary metabolite

Produced during the stationary phase.

Not produced by all microorganisms

Have no direct  relationship to synthesis  of cell material and  natural growth

Include antibiotics, toxins and alcohol

In this example the secondary metabolite is

2. How does the secondary metabolite been produced?

Produced by streptomycin when one or more nutrients is depleted. In this case, aminoglycoside

3.Relate primary and secondary metabolism of the secondary  metabolite

Primary metabolism is the phase where the microorganisms grow. The primary metabolite is produced and used by the microorganisms for their growth. Then, the secondary metabolism is the phase where the mature microorganisms that grow in primary metabolism start to produce secondary metabolites. In this case, the seconadry metabolite is streptomycin and the metabolism is biosysynthesis of streptomycin, with D-glucose as the substrate.

4) Which microbes is it from and what other microbes produce this secondary metabolite?

Streptomyces griseus, no other microbe known has been known to produce this metabolite as of now

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Topic: Steroid Biosynthesis

Members:
1. Lois Tzu Hui
2. Nur Amilia
3. Lau Lee How
4. Shazleen Natasha

1) secondary metabolite,

Yes, it is secondary metabolite.

2) How does the secondary metabolite been produced?
Produce from the precursor, Acetyl-CoA via phosphorylation, decarboxylation and reduction.

3) Relate primary and secondary metabolism of the secondary  metabolite
Primary metabolite produced from log phase, secondary metabolite produced from stationary phase. Secondary metabolites are derived from primary metabolism, they do not make up basic molecular skeleton of the organism. Its absence does not immediately curtail the life of an organism, a feature contrary to primary metabolite, but survival of the organism is impaired to a larger extent.

4) Which microbes is it from and what other microbes produce this secondary metabolite?
Stigmatella aurantiaca

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Group 1 - Activity 1 (Phototroph) & 2 (Secondary Metabolism - Carotenoid) & 3(Chemolithotrophy)

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Group 1 - Activity 1 (Phototroph) & 2 (Secondary Metabolism - Carotenoid) & 3(Chemolithotrophy)

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- Activity 1 (Phototroph)
- Activity 2 (Secondary Metabolism - Carotenoid)
- Activity 3 (Chemolithotrophy)

Group Members:
(1.) Azrie Fauzy Bin Abdullah
(2.) Cheong Jing Ning
(3.) Hue Seow Yie
(4.) Low Wen Yen
(5.) Nur Azzasyazlin Binti Alhakim

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Group 1 (DNAwesome) - Activity 1 & 2 & 3

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1. Tan Jiayong
2.imran
3.Kek jazz yee
4.Ameera
5.Syuhaidah

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Topic: Tetracycline

Members:
1. Nur Aisyah
2. Zi Ning
3. See Ting
4. Fatin Nabilah
5. Patrick

1. secondary metabolite

Tetracyclines

Tetracycline is a broad-spectrum naphthacene antibiotic

2.  How does the secondary metabolite been produced?

Can be produced by fermentation

produced by the Streptomyces genus of actinobacteria

produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens

The starting point for the synthesis was the enantiomerically-pure ester,  prepared by fermentation of benzoic acid (1) to the 1,2-dihydrodiol

16 is the tetracyclines

Reference: https://www.organic-chemistry.org/Highlights/2005/07November.shtm

Almost all marketable tetracyclines are produced by partial syntheses from chlortetracycline, tetracycline, oxytetracycline, and demeclocycline.

Carbon sources are waste materials from agriculture like sweet potatoes, corncobs, peanut shells, cassava, or pineapple peels.

The tetracyclines are derived by the polyketide biosynthesis pathway, in which initially nonaketidamide is built from malonamoyl-CoA, then aromatized stepwise, and finally selectively hydroxylated and oxygenated by oxygenases

Reference: https://link.springer.com/article/10.1007/s40828-021-00138-x

3. Relate primary and secondary metabolism of the secondary metabolite

Primary metabolism is Ester

Benzoic acid + 1,2-dihydrodiol -> Ester, then ester is used to produce tetracycline

Reference: https://pubs.acs.org/doi/10.1021/ja052151d

Aromatic polyketides are an important class of medically relevant secondary metabolites: the anticancer agent daunorubicin and the tetracycline antibiotics belong to this class. Their production occurs using a type II, or iterative, polyketide synthase.

4. Which microbes is it from and what other microbes produce this secondary metabolite?

Streptomyces

Chlamydiae spp.

Mycoplasmas

Other microbes that produce secondary metabolites are Bacillus subtilis and Aspergillus spp.

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Members:
1. Bryan
2. Li teng
3. Isya
4. Farrah
5. Talitha

Topic: Streptomycin

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Topic: Antibacterial agent
1. Kok liang
2. Angelica
3. Wei Yi
4. Azlia
5. Suhail

1) Secondary metabolite,

Streptomycin

2) How does the secondary metabolite been produced?

https://www.genome.jp/pathway/map00521

The secondary metabolite which is streptomycin is produced by certain strains of Streptomyces griscus.

Streptomycin consists of aminocyclitol (streptidine), 6-deoxyhexose (streptose), and N-methyl-L-glucosamine moieties, which are formed by the pathway of independent biosynthetic.

All of the moieties are derived from D-glucose. The streptidine moiety is synthesized via myo-inositol, which is then oxidized at C-1 and transaminated to give scyllo-inosamine.

3) Relate primary and secondary metabolism of the secondary  metabolite

Primary metabolism included in streptomycin synthesis:

D-glucose : glycolysis

1L-myo-Inositol-1P : Inositol phosphate metabolism

https://www.genome.jp/pathway/sco00521

From the primary metabolism which is glucose, then the glucose converts to the D-glucose 6-phosphate. The conversion of D-glucose 6-phosphate to 1L-myo-inositol 1-phosphate is catalyzed by 1L-myo-inositol 1-phosphate synthase (MIP synthase).

4) Which microbes is it from and what other microbes produce this secondary metabolite?

Soil microbes ~ Streptomyces spp. (E.g Streptomyces griseus)

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Topic: PHA

Members:
1. Hong Siew Yi
2. Nur Nadhrah
3. Nurul Sakinah
4. Ho Pei Yi
5. Mashitoh

1) Secondary metabolite

Secondary metabolites are compounds produced during secondary metabolism (stationary phase) and the compounds produced have no direct relationship to the synthesis of cell material and natural growth. In this case, the secondary metabolite is PHA.

2) How does the secondary metabolite been produced?

Secondary metabolites are produced by microorganisms when one or more of the nutrients in the culture medium is depleted. In this case, PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources.

3) Relate primary and secondary metabolism of the secondary metabolite

The primary metabolism involved in this case are biosynthesis of carbohydrates, De novo fatty acid synthesis and fatty acid b-oxidation. The primary metabolites produced (acetoacetyl-CoA, ( R)-3-hydroxyacyl-ACP and (S)-3-hydroxyacyl-ACP) from these 3 primary metabolisms act as a precursor/intermediate to initiate the secondary metabolism and produce secondary metabolites (PHA).

4) Which microbes is it from and what other microbes produce this secondary metabolite?

In this case, the microbe used is from Pseudomonas sp. while other PHA-producing bacteria are Cupriavidus necator and Bacillus megaterium.

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Topic: Penicillin

1.HANNAH NADIAH BINTI ZULKEFLI
2.Pua Lei Wen
3.Norazrin Anis Bt Rosman
4.TAN JIA HAO
5.LEE XIN YI

1) secondary metabolite,
Penicillin, a secondary metabolite produced near the onset of the stationary phase.

2) How does the secondary metabolite been produced?
Overall, there are three main and important steps to the biosynthesis of penicillin G (benzylpenicillin).

The first step is the condensation of three amino acids—L-α-aminoadipic acid, L-cysteine, L-valine into a tripeptide. Before condensing into the tripeptide, the amino acid L-valine must undergo epimerization to become D-valine. The condensed tripeptide is named δ-(L-α-aminoadipyl)-L-cysteine-D-valine (ACV). The condensation reaction and epimerization are both catalyzed by the enzyme δ-(L-α-aminoadipyl)-L-cysteine-D-valine synthetase (ACVS), a nonribosomal peptide synthetase or NRPS.

The second step in the biosynthesis of penicillin G is the oxidative conversion of linear ACV into the bicyclic intermediate isopenicillin N by isopenicillin N synthase (IPNS), which is encoded by the gene pcbC. Isopenicillin N is a very weak intermediate, because it does not show strong antibiotic activity.

The final step is a transamidation by isopenicillin N N-acyltransferase, in which the α-aminoadipyl side-chain of isopenicillin N is removed and exchanged for a phenylacetyl side-chain. This reaction is encoded by the gene penDE, which is unique in the process of obtaining penicillins.

3) Relate primary and secondary metabolism of the secondary metabolite

Penicillin is a secondary metabolite of certain species of Penicillium and is produced when the growth of the fungus is inhibited by stress. It is not produced during active growth. Production is also limited by feedback in the synthesis pathway of penicillin.

α-ketoglutarate + AcCoA → homocitrate → L-α-aminoadipic acid → L-lysine + β-lactam

The by-product, l-lysine, inhibits the production of homocitrate, so the presence of exogenous lysine should be avoided in penicillin production.

4) Which microbes is it from and what other microbes produce this secondary metabolite?

Originated from Penicillium mold. Other microbes such as genetic engineered yeast can produce penicillin in laboratory

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Topic: Biocement

Members:
1)Anis
2)Adlina
3)Kai Xuan
4)Yong Zi

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Topic: Biopecticide

Members:
1. YEOH SHIR LING A18SC0495
2. ANIS SYAKIRA MOHAMAD A18SC0379
3. YAP YING YAN A18SC0493
4. STEVEN ANAK LILOI A18SC0483
5. NORARINA SURAYA MOHD RAZALI A18SC0431

1) secondary metabolite,

Thuringiensin (Thu), also known as β-exotoxin, is a thermostable secondary metabolite secreted by Bacillus thuringiensis. The chemical formula of Thu is C22H32O19N5P, and it is composed of adenosine, glucose, phosphoric acid, and gluconic diacid.

2) How does the secondary metabolite been produced?

Secondary metabolites are produced by microorganisms when one or more of the nutrients in the culture medium is depleted. In this case, the second metabolite of bacillus thuringiensis is produced during the sporulation phase. After the ingestion, the crystals are solubilized in the insects’ midguts in the alkaline condition. It will be proteolytically converted into a toxic core fragment. Then, the activated toxins bind to the receptor of apical microvillus membrane of epithelial midgut. The oligomerization occurs when the toxins enter the membrane. By increasing the cationic permeability of the membrane, the oligomers form pores or ion channels on it. Due to the free flux of ions, the membrane transport and cell lysis are disrupted. So, the insect dies.

3) Relate primary and secondary metabolism of the secondary metabolite

The primary metabolism is the synthesis of the key precursor, gluconic diacid, using the products of thus, thuC, and thuD.  Gluconic acid is subsequently oxidized to gluconic diacid by UDP-glucose dehydrogenase (ThuD), which can directly oxidize a hydroxyl group to a carboxyl (ic) group. The secondary metabolism of secondary metabolite is gluconic diacid is bound to the ACP region of the nonribosomal peptide synthetase Thu2. A hydroxyl group on the C-1 residue of gluconic diacid forms a thioester bond with a sulfhydryl group of the ACP protein. Next, a UDP-glucose moiety is added onto the gluconic diacid-ACP complex by glycosyltransferase ThuF, and a 1,5-glycosidic bond is formed between glucose and gluconic diacid.

4) Which microbes is it from and what other microbes produce this secondary metabolite? (anis)

Bacillus thuringiensis

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Members:
1. Asyura
2. Hui Nee
3. Lok Yee
4. Taib
5. Zen Dong

TOPIC 1: Biosurfactant - Rhamnolipids (G8)

1) The secondary metabolite is rhamnolipids.

2) The rhamnolipids are produced by microbial production. Fermentable sugar (glucose and lactose) or glycerol are used as a carbon source.

3) Primary metabolites are crucial for various metabolic activities as some act as a substrate for these processes, while others act as catalysts. Based on the figure above, acetyl co-A and glucose-6-phosphate is the primary metabolites derived from the primary metabolism. These primary metabolites can be converted into the precursors required for rhamnolipids synthesis.

4) Mainly produced by Pseudomonas aeruginosa. But, P. putida, P. fluorescens and Burkholderia thailandensis E264 can also produce rhamnolipids. Rhamnolipids can also be produced using sugars as carbon sources by other non-pathogenic strains.

TOPIC 2: Chemolithotrophy

1) In chemolithotrophs, the compounds – the electron donors – are oxidized in the cell, and the electrons are channeled into respiratory chains, ultimately producing ATP and NADH. The ATP and NADH may be used to convert carbon dioxide to carbohydrate.
Besides, chemolithotrophs contribute greatly to the chemical transformation of elements. For example convert ammonia to nitrate or sulfur to sulfate that continually occur in ecosystems.

2) Acidophilic sulphur-oxidizing lithotrophs have higher acid-tolerance as they are widely distributed in acidic sulfur-containing environments on land or in the sea, including soil, sediments, hot springs, iron-sulfur mineral deposits and acid mine drainage.  They are mostly acidophilic as acidophiles have the remarkable capability of oxidizing various reduced inorganic sulfur compounds (RISCs) to obtain electrons for carbon dioxide fixation.

3) Rusticyanin plays its role in the electron transfer of the respiratory pathway of Fe²⁺ in T. ferrooxidans (Acidithiobacillus). The respiratory oxidation of ferrous ions to ferric ions will produce three protons for every ferrous ion oxidized. Rusticyanin can be found in the periplasm of the mentioned bacteria, it also can be found in the surface of the T. ferrooxidans, in the form of membrane-bound.

4) The inorganic electron donor for Nitrosomonas is ammonia (NH⁴⁺) while inorganic electron donor for Nitrobacter is Nitrite (NO²⁻)

5) Nitrifying bacteria use carbon dioxide as carbon source.

6) Anammox is known as anaerobic ammonium oxidation. Anammox reaction is ammonium oxidation to dinitrogen gas using nitrite (electron acceptor) without the presence of oxygen. Aerobic nitrification; In the presence of O², the produced NO can be (re)oxidized to NO²

# data[].attributes.body
Name : Patrick Ng Soon Chait
Lives : Muar, Johor
Likes : Reading
Ambition : Live with a healthy life mentally and physically

# data[].attributes.body
Name: Anis Syakira bt Mohamad
Lives : Pasir Gudang, Johor
Likes : Yellow, music, skz, kpop , ice cream, stationery things
Ambition : Good lifestyle, do things I like, can buy anything for my family.

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Name: Nurul Sakinah binti Mohd Zaini
Lives: Kuala Kangsar, Perak
Likes: Art, cats, music, coffee
Ambition: Becoming a hot single rich aunt

# data[].attributes.body
Name : Bryan Chan Yin Kang
Lives : Seri Kembangan, Selangor
Likes : Hanging out with friends, Gaming and Food
Ambition : Having a comfortable lifestyle and striving to be the best I can be

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Group 2-  Biosynthesis pathway of kojic acids

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Group 2-  Biosynthesis pathway of kojic acids

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1. Tan Jiayong
2.imran
3.Kek jazz yee
4.Ameera
5.Syuhaidah

1) Secondary metabolites?

Kojic acid is a secondary metabolite isolated from Aspergillus fungi that has demonstrated skin whitening, antioxidant and antitumour properties among others.

2) How does the secondary metabolites been produced?

Fermentation of glucose. glucose is converted to d-gluconic acid by glucose dehydrogenase and d-gluconic acid is converted to kojic acid by gluconate dehydrogenase.

3) Relate primary and secondary metabolism of the secondary  metabolite?
Kojic acid biologically occurs in the direct fermentation of glucose in A. oryzae. The primary metabolism in the pathway is when the glucose is dehydrogenated by glucose dehydrogenase, to form gluconic acid δ-lactone. At this point, the reaction can continue via two different pathways, both of which carry out dehydrogenation, reduction, and dehydration to lead to the final product of kojic acid.

The two pathways are as shown below:

1. The gluconic acid δ-lactone is first dehydrogenated to 3-ketogluconic acid lactone and then reduced to 3-ketoglucose. Both of these products act as a precursor/intermediary to the secondary metabolism, where 3-ketoglucose is finally dehydrated to form kojic acid.

2. In the other pathway, gluconic acid δ-lactone is both dehydrogenated and dehydrated to oxykojic acid, which acts as a precursor/intermediary to the secondary metabolism, where oxykojic acid is finally reduced to form kojic acid.

4) Which microbes is it from and what other microbes produce this secondary metabolites?

Aspergillus flavus and Aspergillus oryzae

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NUR ANIS NADIA BINTI MOHAMAD SHAH A18SC0440

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NUR ANIS NADIA BINTI MOHAMAD SHAH A18SC0440

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Name : Nur Anis Nadia binti Mohamad Shah

Lives : Bagan Serai, Perak

Likes : Extreme sports, vacation trip, eating and sleeping

Ambition : Being great and manageable in my career with a happy life, have a wide knowledge of my interest

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Name: Kew Kok Liang
Lives: Johor Bahru, Johor
Likes: Video game, electronics
Ambition: Be proud of everything I own

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Nur Ayunie Bt Mohamad Hanafiah (A18SC0442)

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Nur Ayunie Bt Mohamad Hanafiah (A18SC0442)

# data[].attributes.body
Name: Nur Ayunie Bt Mohamad Hanafiah
Lives: Pasir Mas, Kelantan
Likes: Travel, food, animal, sketching and painting
Ambition: Travel around the world

# data[].attributes.body
Name: Lee Jing Wen
Lives: Kajang, Selangor
Likes: Watching drama, cycling, travel
Ambition: Earn money doing things I like

# data[].attributes.body
Name: Koh Zi Ning
Lives: Kuantan, Pahang
Likes: Playing games, watch movies and reading
Ambition: Good health and financial freedom

# data[].attributes.body
Name: Wan Nooranis Binti Wan Zaid
Lives: Kangar, Perlis
Likes: Hiking, Food, Animal, Movies
Ambition: Good health, good job with good environment

# data[].attributes.body
Name : Cheong Jing Ning
Lives : Skudai, Johor
Likes : Travel, Food, Dessert, Baking
Ambition : Financial freedom, travel around the world.

# data[].attributes.body
Name: Hue Seow Yie
Lives: Kulai, Johor
Likes: Reading, drama and listening to music
Ambition: Living with passion and travel around the world.

# data[].attributes.body
Name : Nurul Shazleen Natasha binti Mohd Miskam
Lives : Skudai, Johor.
Likes : Music, drama and movies, food hunting.
Ambition : Able to buy favourite things with my own money and living to the fullest with friends and family.

# data[].attributes.body
Name: Low Wen Yen (Doris)
Lives: Jinjang, KL
Likes: Family, Friendship, Reading, Video games, Photography, Music.
Ambition: To be a happy adult

# data[].attributes.body
Name: Nor Taib Arash
Lives: Kota Bharu, Kelantan.
Likes: Listening to music, cats, and adventure.
Ambition: To be rich and travel around the world and discover hidden gems, and become a singer hahahah.

# data[].attributes.body
Name : Lee Xin Yi

Lives : Skudai, JB

Likes : Drama, Kpop, Travel

Ambition : Travel around the world~

# data[].attributes.body
Name: Lau Lee How
Lives: Kuantan, Pahang
Likes: Watch anime, read novel, play games
Ambition: Travel around the world, stay healthy and live a happy life with my family

# data[].attributes.body
Name: Talitha Labiba
Lives: Semarang, Indonesia
Likes: Listening to music, traveling, coffee
Ambition: Travel around the world with my family

# data[].attributes.body
Name : Fatin Nabilah binti Rakib
Lives : Taiping, Perak
Likes : Sleep, eat, drive
Ambition : Have a stable career with healthy environment, financial freedom

# data[].attributes.body
Name: Nur Isyafatira binti Japri
Lives: Port Dickson, Negeri Sembilan
Likes: Kpop, Kdrama, Youtube, Food, Sleeping, Travel
Ambition: Living outside Malaysia, creating memories and meaningful life, good mind and long lasting health.

# data[].attributes.body
Name: Hon Hui Nee

Lives: Kuantan, Pahang

Likes: Reading, Coffee and Travelling

Ambition: Have a healthy work-life balance and financial freedom in future

# data[].attributes.body
Name: Hannah Nadiah binti Zulkefli
Lives: Melaka
Likes: Good movies and dramas, travel and music
Ambition: Stress free

# data[].attributes.body
Name : Mashitoh binti Ahmad ( can call me Mashi)
Lives : Sungai Petani, Kedah.
Hobbies : Car rides,  food hunting,  watch horror movies
Ambition : Financial freedom, stay healthy and travel around the world.

# data[].attributes.body
Name: Muhammad Suhail Bin Mohd Ariffin
Lives: Kajang, Selangor
Likes: Playing computer games, listening to music, watching movies and anime, drawing portrait/ cartoon, cats, reading manga and fried chicken
Ambition: Travel around the world, do extreme sports, financial freedom

# data[].attributes.body
Name: Kek Jazz Yee
Lives: Kahang, Johor
Likes: Travel, Bubble tea, Baking, Listening to music
Ambition: Financial and time freedom to travel around the world

# data[].attributes.body
Name: A'dlina binti Husny @ Badrul Hisham
Lives: Jerantut, Pahang
Likes: Kpop, Kdrama, singing, variety shows
Ambition: Live happily without any problems or life crisis

# data[].attributes.body
Name: Norazrin Anis Bt Rosman
Lives: Banting, Selangor
Likes: Car rides & myself hehe
Ambition: Be successful and able to help others

# data[].attributes.body
Name: Norarina Suraya Binti Mohd Razali
Lives: Dungun, Terengganu
Likes: Baking, reading, playing chess, watching medical and action series, Jeno
Ambition: Achieve my goals and being happy.

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Name: Chin Lok Yee
Lives: Ipoh,Perak
Likes: Reading novel, baking, travel, sleep😴
Ambition: Financial freedom,stay healthy

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Name : Tan Li Teng
Lives : Butterworth, Penang
Likes : Play online game and rest all day
Ambition : Take part in sky diving and ocean diving

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Name : Nur Amilia binti Ahmad Sukri
Lives : Kepala Batas, Penang
Likes : Drama, Travel, Music, Beaches
Ambition : Being able to make my parents proud, living a good and stable life with good health.

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NAME: Tan Zen Dong

LIVES: Bukit Mertajam, Penang

LIKES: Playing games and basketball

AMBITION: No restrictions on anything

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Name: Mohammad Al-Fayyadh B Md Noor
Lives: Senawang, N.Sembilan
Likes: Cats🐱 , video games💻 , space 🌌, foods🥙, animes
Ambition: Yes, financial freedom, travelling and making my family proud 👨‍👩‍👧‍👦

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Name: Ho Pei Yi
Lives: Kajang, Selangor
Likes: Drama, travel, food
Ambition: Having a good work-life balance in the future, good health

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Name: Hong Siew Yi
Lives: Kuantan, Pahang.
Likes: Sleeping, traveling, watching dramas, reading novels
Ambition: Stay healthy, financial freedom

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Name: Cheong Kai Xuan
Lives: Ipoh, Perak
Likes: Food, Dramas, Travel, Listen to Music, Sleep
Ambition: Stay Motivate toward goals, Stay Healthy, Financial Freedom

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Name: Cheong Wei Yi
Lives: Ipoh, Perak
Likes: Listen to music, Travelling, Dessert
Ambition: Travel around the world

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Name:  Imran Qashfi bin Ismail
Lives: Bandar Seri Iskandar, Perak
Likes: Cats, & food,
Ambition: Financial freedom.

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Name: Nur Nadhrah Bt Mohd Salim

Lives: Sungkai, Perak

Likes: Baking, Arts and Music

Ambition: To make my parents proud, live a great life with stable career and a good health around my loved ones.

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Name: Nur Aisyah binti Md Nizam
Lives: Seremban, N.Sembilan
Likes: Kpop, kdramas, reading books and food.
Ambition: Achieving my goals and have good physical and mental health for a long time

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Name: Jane Seng Zhi Yuan

Lives: Johor Bahru, Johor

Likes: Journal, Art, Cafe Hopping, Travel, Korean and Japanese cultures

Ambitions: Financial freedom, Travel around the world, Keep learning new things

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Name: Lois Lo Tzu Hui
Lives: Kota Kinabalu, Sabah
Likes: Music, anime, salmon, Twice
Ambition: Product specialist / financial freedom

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Name: Angelica Christina Khiu Sing Hui
Lives: Miri, Sarawak
Likes: Playing mobile games, watching drama and reading books.
Ambition: Able to travel around the world.

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Salam & Hye everyone. Good day to all of you guys. For starters, you may call me, Azrie yeah! <3

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Name :~ Azrie Fauzy Bin Abdullah

Lives :~ Tangkak, Johor

Likes :~ Enjoy listening to 'Korean' musics coming from variety of genres, food-hunting sesh (travelling), baking various kinds of savoury & mouth-watering desserts, last but not least, of course playing badminton :))

Ambition :~ Becoming a well-founded & dependable "Personal Shopper", pursuing my career opportunities/paths as a "Food Technologist", and maybe working as a "Researcher" at any given institution(s) as the profession of interest in the future later on, or perhaps in any related fields

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Name: Lee See Ting
Lives: Johor Bahru, Johor
Likes: Reading, drawing, watching anime, listen to music
Ambition: Read as many books as possible, keep my passion in drawing

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- Nurasyura Najla Bt Basri
- Rembau, Negeri Sembilan
- I like makeup, singing and create poster/infographic
- I want to be a Makeup Artists (MUA) and research assistant.

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Name: Noor Farrah Aminah Binti Mukhtar
Lives: Tanah Merah, Kelantan
Likes: Nature particularly beaches and plants, selling plants, cats, sewing, ice cream, taekwondo and anything else that looks interesting.
Ambition: financial freedom, good health, becoming a successful entrepreneur, living a happy life with everyone I care about and becoming a better version of myself in Dunya wal Akhirah

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Name: Steven Anak Liloi
Lives: Kanowit, Sarawak
Likes: Food, Drama, Travel
Ambition: Venture Capital

# data[].attributes.body
Name: Nor Azlia Aziera Binti Abd Shukor

Lives: Labis, Johor

Likes: Travelling, listening to music, cooking, kpop, variety shows and kdramas.

Ambition: Good and stable life.

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Name: Yap Yong Zi
Lives: Skudai, Johor
Likes: Play piano, Art, Reading, Anime
Ambition: Financial freedom

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Name: Ameera Rahaman
Lives: Currently in Skudai
Likes: art, poetry, creative hobbies, travel, music and a little bit of everything
Ambition: To be someone remembered by history

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Name: Puteri Syuhaidah Binti Abdul Rahman
Lives: Ipoh, Perak
Likes: Sherlock Holmes, forensic files, listening to music, cats and pastry.
Ambition: Financial freedom and good health.

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NAME: TAN JIA YONG
LIVES: TAMAN MUNSYI IBRAHIM, SKUDAI JOHOR BAHRU
LIKES: Watching anime and korean movies
Ambition: Financial freedom

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Name: Yeoh Shir Ling
Lives: Bukit Mertajam, Penang
Likes: Travel, eating, cafe, sleeping, coffee
Ambition: Financial freedom

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Hello Dr Siti and friends. I am Pua Lei Wen. Just call me Lei Wen.

Name : Pua Lei Wen
Lives : Layang-Layang, Johor
Likes : Kpop, Handwork, Dessert
Ambition : Good living without working

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Name: Yap Ying Yan
Lives: Seremban, Negeri Sembilan
Likes: travel, animal, adventure, family, mini cooper, lambogini
Ambition: financial and time freedom to get all the things i likes come true.

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I am your course lecturer for SMBT 4183.
Name : Dr Siti Halimah Hasmoni
Lives : Johor Bahru
Likes : Adele, Beaches, cafe, crime docu series and Coffee
Ambition : reading all the books that i bought and Living my dream