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"Come to my woman's breasts,And take my milk for gall, you murdering ministers,Wherever in your sightless substances You wait on nature's mischief! Come, thick night,And pall thee in the dunnest smoke of hell,That my keen knife see not the wound it makes,Nor heaven peep through the blanket of the dark,To cry 'Hold, hold!'"

Here Lady Macbeth calls upon the "murdering ministers" to assist in the murder of Duncan, just like when she wants to become "unsexed" she wants her inner evil to come through so can become able to kill Ducan.
The entire quote is very reminiscent of the unaturalness of the witches and just like the witches she wants to become ambigious in her gender. She also calls for darkness in order to cover over her crime because she is perfectly aware that what she is not a noble action. "Nor heaven to peak through the blanket"
It is interesting that she is not asking the "mudering ministers" for help or for somone else to do it, she is only asking for the personal strength to do what needs to be done.
This tells us alot about Lady Macbeth. She is uncompromising and seemingly fearless as she gathers her strength. She appears determined to do anything to achieve her goal.
It also seems clear that she is loyal to Macbeth and is willing to sacrifice her goodness and her gender to achieve this goal, just based on the letter. Macbeth does not have to convince her.

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"The raven himself is hoarse
That croaks the fatal entrance of Duncan
Under my battlements. Come, you spirits
That tend on mortal thoughts, unsex me here,
And fill me from the crown to the toe top-full
Of direst cruelty! make thick my blood;
Stop up the access and passage to remorse,
That no compunctious visitings of nature
Shake my fell purpose, nor keep peace between
The effect and it!"

Lady Macbeth summons the murderous spirits and calls upon their strengths to murder the king.
She wants them to remove her womanhood and take away tenderness. She wants her blood made thick and to be filled with cruelty. All in all she wants to be cruel and without remorse.
She is delighted over the news of Macbeth's new title. She is sinister because she is planning a regicide.
Also, she is very unscrupulous as she has no hesitations making these plans. Furthermore, she is without any conscience. She is dominating towards Macbeth and almost forces her ideas upon him.

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Duncan shall never see the sun again
She tells Macbeth to act normally and be confident because she fears that people can read his face and see "through" his fear. Macbeth must therefore look like an innocent flower, but be as sinister as a serpent underneath. Lady Macbeth is determined to get the deed done.

She

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https://docs.google.com/document/d/1lBNyWOTWWJRc_qnfzaJRvtmtJvD2NlCjnpQviOHopCM/edit?usp=sharing

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Quotation: O, never
Shall sun that morrow see!
Your face, my thane, is as a book where men
May read strange matters. To beguile the time,
Look like the time; bear welcome in your eye,
Your hand, your tongue: look like the innocent flower,
But be the serpent under't. He that's coming
Must be provided for: and you shall put
This night's great business into my dispatch;
Which shall to all our nights and days to come
Give solely sovereign sway and masterdom.

Lady Macbeth emphasizes the importance of Macbeth’s expression when the king comes to visit. He should look innocent like a flower, but under the surface he should still be sly and devious like a serpent. Under her direction, they will achieve power and mastery of kingship.

These lines show Lady Macbeths ruthless nature and willingness to commit regicide. Furthermore, she wants Macbeth to act like a serpent under the surface and seems sinister in that regard. She seems consumed by the power which it would bring and doesn’t hinder Macbeth from performing an act which they recognize as evil.