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First electron microscope designed and built  first transmission electron microscope by Ernst Ruska and Max Knoll

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Frits Zernike developed phase contrast illumination, allowing the imaging of transparent samples.

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Ernst Ruska built the first scanning electron microscope

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Marvin Minsky patented the principle of confocal imaging.

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Osamu Shimomura, Frank Johnson and Yo Saiga discovered green fluorescent protein

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Godfrey Hounsfield and Allan Cormack develop the computerised axial tomography (CAT) scanner.

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John Venables and CJ Harland observed the electron backscatter patterns (EBSP) in the scanning electron microscope.