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Our awesome group G2 And Our microbe seniors

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Our awesome group G2 And Our microbe seniors

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This time, we geng up with our seniors and chose Trychoderma harzianum as our fungus.

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From Yanesa and luqman

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Provided by luqman. There's even the spores seen in the picture

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Provided by Wem Ying

The fungal has a transformant strain that over expresses the gtt1 gene shows a threefold increase in glucose but not galactose or xylose uptake, a finding which confirms the role of the gtt1 gene in glucose transport.
The gtt1 gene is transcriptionally regulated, being repressed at high levels of glucose.

When carbon sources other than glucose are utilized, gtt1 repression is partially alleviated. Full derepression of gtt1 is obtained when the fungus is grown in the presence of low carbon source concentrations. This regulation pattern correlates with the role of this gene in glucose uptake during carbon starvation.

The cloning of the first filamentous ascomycete glucose transporter is the first step in elucidating the mechanisms of glucose uptake and carbon repression in aerobic fungi.

Gtt1 gene is a gene which is responsible for the uptake of glucose in Trichoderma harzianum.The gtt1 gene is transcriptionally regulated, being repressed at high levels of glucose. When carbon sources other than glucose are utilized, gtt1 repression is partially alleviated. Full derepression of gtt1 is obtained when the fungus is grown in the presence of low carbon source concentrations. This regulation pattern correlates with the role of this gene in glucose uptake during carbon starvation. The cloning of the first filamentous ascomycete glucose transporter is the first step in elucidating the mechanisms of glucose uptake and carbon repression in aerobic fungi. A transformant strain that overexpresses the gtt1 gene shows a threefold increase in glucose but not galactose or xylose uptake, a finding which confirms the role of the gtt1 gene in glucose transport.

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Here are the application of Trychoderma harzianum in our daily life...

1)Bioremedation by mutulism with another fungal, Glomus intraradices biocontrol from plant pathogens.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91202/#__ffn_sectitle

2) In human, it can cause infection in the rental transplant  which is due to the kidney failure cause by the fungal. Thus, cause peritonitis in diabetic patients  ( Guarro,1999)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC85751/#!po=28.4091

From Husna