# data[].attributes.body
Indiana vesiculovirus most prominently causes a vesicular disease in domestic animals that resembles foot-and-mouth disease.

link:https://www.sciencedirect.com/topics/neuroscience/vesicular-stomatitis-virus

# data[].attributes.body
Zaire ebolavirus is a rare but deadly virus that causes fever, body aches, and diarrhea, and sometimes bleeding inside and outside the body.

link: https://www.webmd.com/a-to-z-guides/ebola-fever-virus-infection

# data[].attributes.body
Inject the culture to human blood stream and let the antibodies against the WBC with EBOV antigen.

# data[].attributes.body
Patient's body has achieved immunity towards EBOV.

# data[].attributes.body
The culture is treated with antiviral drug, ribavirin to kill normal VSV present in it since it has no ribavirin resistance gene in its DNA.

# data[].attributes.body
Culture the VSV with human white blood cell (WBC)and let the transducted VSV to infect WBC. The WBC with EBOV antigen could be obtained and could be cultured as a vaccine.

# data[].attributes.body
Infect the transformed mammalian eukaryotic cell with VSV by transduction and let the VSV packages either recombinant DNA or its own DNA.

# data[].attributes.body
The gene of interest of EBOV is linked to the cut MCS plasmid by using DNA ligase.

# data[].attributes.body
Introduce the recombinant DNA plasmid into the mammalian eukaryotic cell by undergoing electroporation.

# data[].attributes.body
Cut the recognition sequence at the multiple cloning site (MCS) plasmid with a ribavirin resistance gene by using the same restriction enzyme, EcoR1 to ensure the complementary 'sticky end' between the MCS plasmid and the targeted gene.

# data[].attributes.body
Determine the targeted gene that encode the information of viral capsid of the EBOV and cut the gene using restriction enzyme, EcoR1.

# data[].attributes.body
Use Reverse transcriptase enzyme, to reverse transcript the viral RNA to complementary DNA (cDNA).

# data[].attributes.body
The viral RNA could be obtained from a bat and be undergoing gel electrophoresis to separate the viral RNA with other macromolecules e.g. DNA or proteins of the infected animals.