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FISH overcomes problems the CCA technique experiences in diagnosis

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FISH overcomes problems the CCA technique experiences in diagnosis

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(Put, Michaux and Vandenberghe, 2014)

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Brown, M. and Wittwer, C. (2000). Flow Cytometry: Principles and Clinical Applications in Hematology. [online] Clinchem.aaccjnls.org. Available at: http://clinchem.aaccjnls.org/content/46/8/1221/tab-figures-data [Accessed 4 Nov. 2016].

Nbt.nhs.uk. (2016). FISH (Fluorescence in situ Hybridisation) | North Bristol NHS Trust. [online] Available at: https://www.nbt.nhs.uk/severn-pathology/pathology-services/bristol-genetics-laboratory-bgl/molecular-cytogenetics/fish [Accessed 6 Nov. 2016].

Fluorescence In Situ Hybridization (FISH). (2013). [video] Abnova.

Jennings, C. and Foon, K. (1997). Flow Cytometry: Recent Advances in Diagnosis and Monitoring of Leukemia. Cancer Investigation, [online] 15(4), pp.384-399. Available at: http://www.bloodjournal.org/content/90/8/2863.full.pdf+html?sso-checked=true [Accessed 1 Nov. 2016].

Mehta, A., Hoffbrand, A. and Gschmeissner, S. (n.d.). Haematology at a glance.

Moore, G., Knight, G. and Blann, A. (n.d.). Haematology.

Put, N., Michaux, L. and Vandenberghe, P. (2014). The cytogenetic and molecular diagnosis of haematological malignancies: an overview of current techniques. Belgian Journal of Hematology, 5(1).

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Overview of practical understanding. (Fluorescence In Situ Hybridization (FISH), 2013)

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Fluorescence In Situ Hybridization (FISH)

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( http://www.abnova.com ) - FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. You can use FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library.

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Explains patient presentation and abnormalities in the full blood count (Moore, Knight and Blann, n.d.)

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(Put, Michaux and Vandenberghe, 2014)

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FISH overcomes problems the CCA technique experiences in diagnosis

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FISH overcomes problems the CCA technique experiences in diagnosis

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It can be used at higher resolutions and at metaphase stages. (Nbt.nhs.uk, 2016)

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1. Home

2. Severn Pathology

3. Pathology Services

4. Bristol Genetics Laboratory (BGL)

5. Molecular Cytogenetics

6. FISH (Fluorescence in situ Hybridisation)

What is FISH?

FISH is a rapid, specific, sensitive and versatile technique based on DNA probes annealing to specific target sequences of sample DNA

It utilises fluorescently labelled reporter molecules to detect losses, gains or rearrangements at particular loci and to confirm the presence or absence of a particular genetic aberration, when viewed under fluorescence microscopy

It is a technique that enables the detection of DNA sequences by exploiting the annealing properties of these molecules

The fluorescently labelled probe is homologous to the specific region of interest on the patient DNA

It can be applied to a range of tissue types including bone marrow, blood, AF and CVS samples, fresh tissue, pleural fluid, cerebral spinal fluid and formalin fixed paraffin embedded sections (FFPE)

It can be used to target DNA in interphase nuclei or metaphase chromosomes, i.e. from uncultured and cultured material
Why do we use FISH?

FISH has a higher resolution than conventional cytogenetics and therefore enables the detection of cryptic (not visible by conventional cytogenetics) deletions or other chromosomal rearrangements. It is also used to complement other techniques

FISH enables the rapid analysis of large numbers of cells for a specific target and therefore is more sensitive than conventional cytogenetic analysis ​

FISH does not require viable cells so can be applied to fixed material, for example FFPE tissue sections

At the BGL FISH is applied for oncology applications.

BGL use commercially prepared CE marked or validated in-house probes and new probes are incorporated into the repertoire as they become available.

On request, cell suspension may be kept for two years for possible future FISH testing.

Please contact Bristol Genetics Laboratory for up-to-date information regarding new probes.

Last updated: 25/11/2016

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FISH (Fluorescence in situ Hybridisation) at Bristol Genetics Laboratory

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Home Severn Pathology Pathology Services Bristol Genetics Laboratory (BGL) Molecular Cytogenetics FISH (Fluorescence in situ Hybridisation) What is FISH?

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Side Scatter Plots distinguish between multiple populations

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Side Scatter Plots distinguish between multiple populations

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(Brown and Wittwer, 2000)

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(Jennings and Foon, 1997)

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covers mechanisms of malignant transformation and laboratory features involved in diagnosis. Pages 58 and 65. (Mehta, Hoffbrand and Gschmeissner, n.d.)