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GFP in general

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Hydrophobic interaction chromatography

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SDS-PAGE

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Western blot

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Buffers

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TE buffer (?) instead of water in Lysate

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Protein purification

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Hypothesis

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Western blot

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Pictures from column chromotography

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Tagging of proteins

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Recombinant proteins

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Why do we want to produce proteins? Background

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Why do we want to produce proteins? Background

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GFP bound to proteins and free gfp in solution

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Did we observe other protein bands in the gel? Why?

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Did we observe other protein bands in the gel? Why?

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Fractions from hic

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Production and detection

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Expections and result

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Possible improvements