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To investigate the effect of temperature on activity of catalase in potato tuber tissues.

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Temperature of solution in the small beaker

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Investigate effect of temperature an activity of enzyme catalase in potato tuber tissue.

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to investigate the effect of temperature on the activity of the enzyme catalase in potato tuber tissue.

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Temperature

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temperature.

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temperature

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- Size of potato tuber tissue
- pH value
- vol of H2O2
- conc. Of catalase
- volume of catalase

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Thermometer
Tripod stand
Bunsen burner

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to investigate the effect of temperature on the activity of the enzyme catalase in the potato tuber tissue.

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1. potatoes from the same tuber
2. volume of catalase
3. volume of hydrogen peroxide
4. concentration of catalase
5. size of potato

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investigate how temperature effects the rate of activity of the enzyme catalase

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temperature

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Volume of hydrogen peroxide
Size of the potato tuber tissue
Concentration of hydrogen peroxide
pH of the solution
Duration of heating the solution in the beaker

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pH, size of potato tuber tissue, concentration of enzyme catalase, volume of hydrogen peroxide, volume of catalase

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Thermometer
Tripod stand
Lighter
Bunsen burner

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pH level of enzyme
length of potato cylinder
volume of hydrogen peroxide
duration of experiment
volume of catalse used

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Solutions of Discs should contain the same pH values but different temperatures. (E.g 20’C, 30’C, 40’C, 50’C)

Place different discs containing same hydrogen peroxide solution and potato tuber tissue with different temperatures.

As soon as the disc reaches the bottom of the tube, start the stop watch.

Stop timing as soon as any part of the disc move up from bottom of the tube. Record the time taken in seconds in your table.

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thermometer
retort stand
beaker
bunsen burner

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thermometer, bunsen burner, tripod stand, water bath

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thermometer and bunsen burner

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keep the pH constant and heat the test tube

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1. Put the beaker containing the 2 discs on the tripod stand and place the thermometer into the beaker.

2. Using the Bunsen burner,  heat up the solution for 5 minutes. Start the stopwatch immediately as the Bunsen burner starts heating up the solution.

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1. use a 5cm3 syringe to put 5cm3 of hydrogen peroxide solution into a test tube
2. boil a beaker of water to 30 degrees celcius

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step 1: step remain unchange
step 2: prepare water bath
step 3 : record temperature at different intervals
10 , 20 , 30, 40, 50
4: count the number of water bubbles produced
5: repeat step 3 and 4 , 3 more time