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DNA Content and Cell Cycle Analysis using Flow Cytometry

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DNA Content and Cell Cycle Analysis using Flow Cytometry

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DNA content cell cycle analysis using flow cytometry

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Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data

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Pre-prac

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Molecular Probes Tutorial Series—Introduction to Flow Cytometry

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Pre-prac

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Molecular Probes Tutorial Series—Overview of  Filters and Light Sources

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Pre-prac

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Molecular Probes Tutorial Series— Anatomy of Fluorescence Spectra

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Pre-prac

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                                                              KEY CONCEPTS 

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                                                              KEY CONCEPTS 

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You should understand each of the following concepts to understand Flow Cytometry

FLUIDICS SYSTEM: HYDRODYNAMIC FOCUSING

OPTICS & DETECTION: LIGHT SCATTERING
FLUORESCENCE EMISSION
DETECTORS
OPTICAL FILTERS

SIGNAL PROCESSING: PHOTODETECTOR
ANALOGUE TO DIGITAL CONVERTERS
PARAMETERS
EVENTS

PRINCIPLES OF FLUORESCENCE: FLUOROCHROMES & FLUORESCENT PROBES
STOKES SHIFT
FLUORESCENCE COMPENSATION
SPECTRAL OVERLAP
GATES & REGIONS: GATING
DENSITY PLOT
HISTOGRAM

IMMUNOPHENOTYPING

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Surface and Intracellular Antigen detection by flow cytometry

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Surface and Intracellular Antigen detection by flow cytometry

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Dialogue Box - Let us be kind, professional & courteous

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Dialogue Box - Let us be kind, professional & courteous

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Lecturer: Are there any concepts that were unclear during the practical lecture? Drop a note below (anonymously).

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                                                    MODULE: FLG330

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                                                    MODULE: FLG330

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Flow Cytometry Practical Lecture: Interactive platform for students & lecturer