# data[].attributes.headline
How temperature affects bacterial growth

# data[].attributes.subject
How temperature affects bacterial growth

# data[].attributes.body
1. Why temperature has a significant effect on living organisms

2. Define minimum, optimum and maximum growth temperature

3. What is the temperature range for psychrophiles, Psychrotrophs, Mesophiles, Thermophiles, Hyperthermophiles

4. Give an example for each group

5. Which one of  the above groups is most likely to cause food spoilage

6. What is the deference between thermoduric bacteria and thermophiles

•Class discussion/feedback

# data[].attributes.headline
1. Why temperature has a significant effect on living organisms?

# data[].attributes.subject
1. Why temperature has a significant effect on living organisms?

# data[].attributes.body
Temperature impacts microbial growth. Most microbes grow optimally within a certain temperature range dictated by the ability of proteins within the cell to function. In general, at low temperatures, microbes grow slower. At higher temperatures, microbes grow more quickly.

# data[].attributes.attachment_link.readable_text
figures by Molly Sargen and Nicholas Lue
Microbes (also known as microorganisms) are everywhere: on surfaces we touch, in the air we breathe, and even inside us. As suggested by the name, all microbes are too small to be seen without a microscope. Beyond size, microbes are incredibly diverse. Microbes include bacteria, fungi, and protists. To be able to live harmoniously with all of these microbes, we implement numerous practices to control their growth.

On one hand, there are many harmful microbes we try to avoid. Consider the pathogenic bacteria Listeria monocytogenesand Salmonella entericathatcause foodborne illnesses. We use refrigeration to slow the growth of microbes like these and keep food safe to eat. On the other hand, some microbes perform useful functions and we intentionally cultivate them. For example, bakers use warm temperatures to promote the growth of the yeast Saccharomyces cerevisiaeto make bread dough rise. Some microbes can be both harmful or beneficial in different conditions. For instance, Escherichia coli can cause gastrointestinal illness when ingested but can produce life-saving synthetic insulin in an industrial setting. Understanding the fascinating growth of microbes helps us develop practices to maintain balanced interactions with these microorganisms.

Figure 1: Microscopy reveals the intricate features of microbes. It takes at 400-1000X magnification to see these organisms clearly with a microscope. From left to right, organisms and size: Salmonella enterica (1 μM), Saccharomyces cerevisiae (10 μM), and Escherichia coli (1 μM). Image sources: S. enterica, S. cerevisiae, E. coli

Features of microbes

Microbes are diverse creatures. Many have unique features and capabilities, yet they share a few common characteristics (Figure 2). Most microbes are made of only one or a few cells. Every microbial cell is surrounded by a cell membrane. The membrane controls the movement of material in and out of the cell. This allows the cell to bring in important material, like nutrients, while expelling waste. Some microbes are also surrounded by a cell wall. The wall provides a structure to enclose the internal components of the cell. Within its interior, each cell carries the DNA encoding its genome. Other structures in the cell perform metabolic functions that are essential for life.

Figure 2: Features of a Microbial Cell. This diagram of a bacterial cell shows the essential features of a microbial cell including DNA, a cell membrane, and the essential components within the cell. This cell has a cell wall and also flagella (an appendage some bacteria use for movement).

Mechanisms of microbial growth

Microbial growth refers to an increase in number of cells rather than an increase in cell size. Many microbes (including Escherichia coli, Salmonella enterica, and Listeria monocytogenes) are unicellular, meaning they are made of only one cell. The size of any unicellular microbe is limited by the capacity for the essential components of the cell to support its survival. For example, the integrity of the cell wall is impaired when cells become too large. The solution to growing despite limits on cell size is for cells to divide or produce new cells from the original cell. Therefore, the population grows although the size of the individual members of the population remains stable.

Most commonly, a single-cellular microbe divides into two identical new cells during one growth cycle (Figure 3a). The original cell, called the parent cell, makes a copy of its DNA and generates enough material to build the membrane, wall, and molecular machines for two cells. The parent cell slightly increases in size to accommodate these additional materials. Then, the parent cell begins to contract at the middle and a new piece of cell wall is assembled at the site of contraction. This process continues until the parent cell is split into two cells with complete cell walls. The resulting cells are called the daughter cells. Because both daughter cells are identical, cell division is also called replication. Replication in this manner leads to a rapid increase in the number of cells as each daughter cell begins the cycle again by acting as a parent cell. Cell division can look slightly different for microbes with different shapes (Figure 3b), but the key principles remain the same.

As long as the conditions are favorable, one cell produces two new cells in a continuous cycle. Every cycle doubles the number of cells in the population. This is known as exponential growth. Depending on the conditions, the division cycle of E. coli can be as short as 20 minutes. This rapid division leads to a rapid increase in the population size (Figure 3c). Eventually, the population is large enough to have an impact we can detect, such as formation of a physical structure. In a research lab, this might be a " colony," a mound of bacteria on solid growth media. You might notice this as plaque on your teeth. It takes over a million bacteria to form a visible structure, but this can occur in only about eight hours when conditions are optimal for E. coli.

Figure 3: The population increases exponentially as cells divide. Microbes with different shapes divide similarly.

Some microbes produce new cells asymmetrically. In this situation, one parent cell produces a single daughter cell by a process called budding. During budding the parent cell develops a small protrusion known as the bud. The materials necessary to support a new cell are sent into the bud, which eventually splits from the parent cell to form a new daughter cell. The parent cell continues to make buds, but the budded daughter cells do not divide. Buds can remain connected in a chain or separate into individual cells (Figure 4). Saccharomyces cerevisiae, a yeast used to make bread, is a budding yeast.

There are also multicellular microbes, like algae and the fungi that form molds. For these microbes, multiple cells work together to keep the organism alive. Each cell might perform slightly different functions for the organism. When a parent cell divides, each daughter cell begins to perform specific functions based on its surroundings. The entire organism grows as new cells form and take on new functions. This is similar to the way larger organisms, like animals, grow.

Figure 4: Some cells use budding to produce daughter cells. A parent cell produces small protrusions called buds.

Factors affecting microbial growth

All types of microbial growth are heavily impacted by environmental conditions. One of the most critical factors for microbial growth is the availability of nutrients and energy. Microbes need carbohydrates, fats, proteins, metals, and vitamins to survive, just like animals. The process of using nutrients and converting them into cellular material requires energy. Every microbe has unique nutritional requirements depending on the types of molecules it is capable of making for itself. Most microbes are fairly robust, meaning they can find a way to grow in a variety of nutritional conditions. Nonetheless, microbes grow more slowly when nutrients are limited.

Temperature also impacts microbial growth. Most microbes grow optimally within a certain temperature range dictated by the ability of proteins within the cell to function. In general, at low temperatures, microbes grow slower. At higher temperatures, microbes grow more quickly. For instance, pathogens often grow best at normal body temperature, but slowly at cooler temperatures outside the body or when body temperature increases during a fever. Extremely high temperatures usually denature the components required for the cells to survive and are lethal for many microbes. Nonetheless, a few exceptional microbes actually prefer to grow at very high temperatures or very low temperatures. These microbes, known as extremophiles, can grow near hydrothermal vents where the temperature is above boiling or surrounded by solid ice.

Even when nutrients are available and the temperature is right, many other environmental factors can influence the growth of microbes. These include acidity, availability of water, and atmospheric pressure. Each microbe prefers a range of properties for multiple features of the environment. Overall, microbes typically grow best at a specific set of conditions and less well at other conditions (Figure 5). Specific preferences for growth are as diverse as the types of microbes.

Figure 5: Microbes grow well within a specific range of conditions for multiple environmental variables. Some microbes can tolerate a wide range of conditions, while others require a specific condition to grow well. Sometimes the conditions that permit growth overlap.

Methods of controlling microbial growth

Decades of research have developed the current understanding of microbial growth to establish the principles outlined above. Establishing common principles allows us to target broad groups of microbes, while unique requirements for growth allows us to target specific microbes. This knowledge enables the control of microbial growth that facilitates many of our interactions with microbes today.

Many methods of control seek to eliminate harmful microbes from foods or equipment. For example, high temperature is often used to kill microbes during cooking or through processes like pasteurization. In this way, potentially harmful microbes are broadly eliminated from the food product making it safe to consume and store. Similarly, chemicals in disinfectants can damage or kill microbes broadly on surfaces. Alcohols like ethanol and isopropanol damage the cell membranes. Without this protective structure, microbes cannot control what enters or exits the cell. Subsequently, microbes cannot retain important nutrients and water. Alternatively, hydrogen peroxide damages structures within the cell. As hydrogen peroxide decomposes, it forms molecules known as free radicals that damage proteins and DNA. Meanwhile, we also use soaps to physically remove microbes from surfaces. The chemical properties of soaps and physical force applied when wiping a surface dislodges the microbes.

When microbes cannot be completely eliminated from a material, such as food products that cannot be heated to high temperatures, measures can be taken to mitigate the growth of microbes. Recognizing how temperature impacts growth, supports the importance of refrigeration. As mentioned, cold temperatures slow the growth of microbes, so refrigeration can delay the growth of microbes in these food products. As described above, microbes can replicate as quickly as every 20 minutes leading to visible growth within only a few hours. At a lower temperature, the cells may divide only once every few hours and it will take multiple days to see visible growth.

Alternatively, when we want to take advantage of microbes, we try to optimize the conditions for their growth. This is why yeasted dough is left at a warm temperature to allow the yeast to grow rapidly. If the dough is refrigerated, it takes much longer to rise. Similarly, to use E. coli for insulin production, we provide bacteria with specific nutrients that facilitate growth.

Given the diversity of microbes in the world, it's clear that there is still so much we don't know about how they grow. Continuing to better understand microbial growth will help us live safely with the microbes in our community and make use of their unique capabilities.
Molly Sargen is a PhD student in the Biological and Biomedical Sciences Program at Harvard Medical School. Nick Lue is a PhD student in the Chemical Biology Program at Harvard University.
Cover image: "Bacteria sample inside petri dish for biotechnology study" by IRRI Images is licensed under CC BY-NC-SA 2.0

For More Information:

Interested in understanding different types of microbes? Try this page.

Learn more about the structure of bacterial cells here.

For more details about cell division, see this page.

For more details about mechanisms of microbial growth, read this article.

To learn more about chemical disinfectants, check out this information from the CDC.

# data[].attributes.attachment_link.description
Coexisting with microbes involves understanding not only how to kill them, but also how they grow.

# data[].attributes.headline
2. Define minimum, optimum and maximum growth temperature

# data[].attributes.subject
2. Define minimum, optimum and maximum growth temperature

# data[].attributes.body
Minimum Growth Temperature: The lowest temperature at which a bacterial species can still carry out basic metabolic functions and reproduce, although at a very slow rate.

Optimum Growth Temperature: The temperature at which a bacterial species experiences the highest growth rate and is most metabolically active.

Maximum Growth Temperature: The highest temperature at which a bacterial species can still grow and reproduce, but beyond which its metabolic and enzymatic activities are compromised.

Different bacterial species have different temperature preferences, leading to the classification of bacteria into temperature categories.

# data[].attributes.attachment_link.title
Optimal Growth Temperature and Intergenic Distances in Bacteria, Archaea, and Plastids of Rhodophytic Branch

# data[].attributes.attachment_link.description
The lengths of intergenic regions between neighboring genes that are convergent, divergent, or unidirectional were calculated for plastids of the rhodophytic branch and complete archaeal and bacterial genomes. Statistically significant linear relationships…

# data[].attributes.body
Psychrophiles: 20 °C for maximal growth, 15 °C or lower for optimal growth, and 0 °C or lower for minimum growth

Psychrotrophs: are organisms can grow below 10 °C and down to about −1 °C, but usually prefer to grow at about 21 °C

Thermophiles: live at temperatures between 60 and 80 ° C

Mesophiles: grow and thrive in a moderate temperature range between 20°C and 45°C.

Hyperthermophiles grow optimally at temperatures between 80 and 110°C.

Reference article: Psychrophilic Bacteria - an overview | ScienceDirect Topics

# data[].attributes.body
Psychrophiles = Listeria, E.coli (raw milk, Enviromental)

Psychrotrophs = Enterobacter kobei (raw milk)

Mesophiles = Clostridium, Staphylococcus, E.coli

Thermophiles = Bacillus, Micrococcus, Streptococcus, Lactobacillus, and Clostridium (Driers)

Hyperthermophiles = Methanopyrus (driers)

# data[].attributes.headline
6. What is the deference between thermoduric bacteria and thermophiles?

# data[].attributes.subject
6. What is the deference between thermoduric bacteria and thermophiles?

# data[].attributes.body
Thermophilic : Bacteria those thrive at 50-110 C growing conditions and this needs for their optimum growth.

Thermoduric : those bacteria which withstand higher temperatures that actually not suitable for their optimal growth.

# data[].attributes.headline
5 Organisms most likely to cause food spoilage

# data[].attributes.subject
5 Organisms most likely to cause food spoilage

# data[].attributes.body
Psychrotrophs are the organisms most likely to cause food spoilage in the dairy industry. This is because they can thrive at the low temperatures in which dairy products are stored, and also contain some heat-resistant strain able to survive pasteurisation.

They can grow at temperatures below 10 °C and down to about −1 °C, although they usually prefer to grow at about 21 °C.

There are many psychrotrophs, but the most important one is Pseudomonas, as 90% of psychrotrophs isolated from cold stored food belongs to this genus.