# data[].attributes.headline
Evaluation of R. Ornithinolytica’ Antimicrobial SusceptibilityIn order to improve the antimicrobial sensitivity, biofield treatment could be used as complementary and alternative treatment strategy against the multidrug-resistant strain of R. Ornithinolytica.

# data[].attributes.subject
Evaluation of R. Ornithinolytica’ Antimicrobial SusceptibilityIn order to improve the antimicrobial sensitivity, biofield treatment could be used as complementary and alternative treatment strategy against the multidrug-resistant strain of R. Ornithinolytica.

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
A Futuristic Treatment Strategy for MDR PathogensThe study suggest about an alternative approach to fight against the multidrug-resistant susceptibility pattern of Raoultella ornithinolytica. For more information, go through the link.

# data[].attributes.subject
A Futuristic Treatment Strategy for MDR PathogensThe study suggest about an alternative approach to fight against the multidrug-resistant susceptibility pattern of Raoultella ornithinolytica. For more information, go through the link.

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica | Mahendra Trivedi | Shrikant Patil | Harish Shettigar | Mayank Gangwar | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
Susceptibility Pattern | Raoultella OrnithinolyticaThe present study suggested that energy treatment has the ability to inhibit the microbial growth, by significantly decreasing the MIC values of 71% & increasing the susceptibility pattern of Raoultella ornithinolytica.

# data[].attributes.subject
Susceptibility Pattern | Raoultella OrnithinolyticaThe present study suggested that energy treatment has the ability to inhibit the microbial growth, by significantly decreasing the MIC values of 71% & increasing the susceptibility pattern of Raoultella ornithinolytica.

# data[].attributes.attachment_link.keywords[]
susceptibility pattern of r. ornithinolytica

# data[].attributes.attachment_link.title
"Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
Biotype Number of R. OrnithinolyticaThe study concluded that, there was an alteration in biotype number of R. Ornithinolytica after the influence of biofield treatment. For more details visit the link.

# data[].attributes.subject
Biotype Number of R. OrnithinolyticaThe study concluded that, there was an alteration in biotype number of R. Ornithinolytica after the influence of biofield treatment. For more details visit the link.

# data[].attributes.attachment_link.keywords[]
biofield - mahendra kumar trivedi - trivedi effect - raoultella ornithinolytica - biofield  treatment

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidru...

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
Biochemical Study of R. OrnithinolyticaRead here about how the biochemical study was performed on R. Ornithinolytica and shows changes in the treated sample as compare to the control sample.

# data[].attributes.subject
Biochemical Study of R. OrnithinolyticaRead here about how the biochemical study was performed on R. Ornithinolytica and shows changes in the treated sample as compare to the control sample.

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so

# data[].attributes.headline
Biofield Treatment on Raoultella OrnithinolyticaThe main aim of this research is to assess the effect of biofield treatment on susceptibility pattern of Raoultella ornithinolytica. Visit the link for further details.

# data[].attributes.subject
Biofield Treatment on Raoultella OrnithinolyticaThe main aim of this research is to assess the effect of biofield treatment on susceptibility pattern of Raoultella ornithinolytica. Visit the link for further details.

# data[].attributes.headline
Alteration in R. OrnithinolyticaIn the research, the biochemical study shows the alteration in the biochemical reaction after the biofield treatment. Follow the link to know more.

# data[].attributes.subject
Alteration in R. OrnithinolyticaIn the research, the biochemical study shows the alteration in the biochemical reaction after the biofield treatment. Follow the link to know more.

# data[].attributes.headline
Effect of Biofield Treatment on Provindencia rettgeriThe present study revealed that biofield treatment could alter the sensitivity of antimicrobials against P. rettgeri. To know more, click on the below given link.

# data[].attributes.subject
Effect of Biofield Treatment on Provindencia rettgeriThe present study revealed that biofield treatment could alter the sensitivity of antimicrobials against P. rettgeri. To know more, click on the below given link.

# data[].attributes.headline
Alternative Energy Treatment on Provindencia rettgeriBased on the research, it is concluded that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to P. rettgeri.

# data[].attributes.subject
Alternative Energy Treatment on Provindencia rettgeriBased on the research, it is concluded that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to P. rettgeri.

# data[].attributes.attachment_link.title
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
The Trivedi Effect | P. rettgeriRead here, about how Mr. Trivedi's unique human energy treatment can affect the characteristics of treated P. rettgeri as compared to untreated samples.

# data[].attributes.subject
The Trivedi Effect | P. rettgeriRead here, about how Mr. Trivedi's unique human energy treatment can affect the characteristics of treated P. rettgeri as compared to untreated samples.

# data[].attributes.headline
Provindencia rettgeri | Impact of Biofield EnergyAfter the biofield treatment, the provindencia rettgeri samples were analysed for the change in phenotypic and genotypic characteristic. Read here to know more about it.

# data[].attributes.subject
Provindencia rettgeri | Impact of Biofield EnergyAfter the biofield treatment, the provindencia rettgeri samples were analysed for the change in phenotypic and genotypic characteristic. Read here to know more about it.

# data[].attributes.attachment_link.title
Molecular Biology Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
Phenotypic Characteristic of Provindencia rettgeriThe study was designed to evaluate the impact of biofield treatment on the phenotypic characteristics of treated sample of P. rettgeri w.r.t untreated.

# data[].attributes.subject
Phenotypic Characteristic of Provindencia rettgeriThe study was designed to evaluate the impact of biofield treatment on the phenotypic characteristics of treated sample of P. rettgeri w.r.t untreated.

# data[].attributes.attachment_link.title
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri | Mahendra Trivedi | Shrikant Patil | Harish Shettigar | Khemraj Bairwa | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
Evaluation of Antimicrobial Susceptibility of P. rettgeriIn the research, investigation of the antimicrobial sensitivity of P. rettgeri was carried out with the help of automated instrument, MicroScan WalkAway®. Get to know more, from the given below link. 

# data[].attributes.subject
Evaluation of Antimicrobial Susceptibility of P. rettgeriIn the research, investigation of the antimicrobial sensitivity of P. rettgeri was carried out with the help of automated instrument, MicroScan WalkAway®. Get to know more, from the given below link. 

# data[].attributes.attachment_link.readable_text
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Volume 4 * Issue 2 * 1000129

ISSN: 2168-9547 MBL, an open access journal

Trivedi et al., Mol Biol 2015, 4:2

http://dx.doi.org/10.4172/2168-9547.1000129

Research ArticleOpen Access

Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular

infections. Present study was designed to evaluate the effect of bioeld treatment on P. rettgeri against antimicrobial

9250) were divided into three groups: Gr.I (control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized). The

Gr.II and III were treated with Mr. Trivedi's bioeld, and then subsequently characterized for antimicrobial susceptibility,

minimum inhibitory concentration (MIC), biochemical reactions, and biotype numbering. The 16S rDNA sequencing

respectively; and 53.3% of tested antimicrobials of Gr.III on day 10. MIC results showed a signicant decrease in MIC

values of 53.1, 56.3, and 56.3% antimicrobials in Gr.II on day 5, Gr.II on day 10, and Gr.III on day 10, respectively, as

compared to control. The signicant changes in biochemical reactions and biotype numbers were also observed in all

vulgaris (Accession No. DQ499636). These ndings suggest that bioeld treatment can prevent the emergence of

absolute resistance of existing antimicrobials to P. rettgeri.

Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad

Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006; E-mail:

Citation: Trivedi MK, Patil S, Shettigar H , Bairwa K, Jana S (2015) Effect of Bioeld

Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri.

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Keywords: Providencia rettgeri; Bioeld treatment; Antimicrobial

susceptibility; Biotype; 16S rDNA sequencing

Presently, several microbes have been acquired the resistance

to number of antimicrobial agents that were successfully treat the

microbial infections earlier. e antimicrobial resistant microbes

whether bacteria, fungi, viruses or parasites can survive in regular

antimicrobial drugs therapy. e frequent and improper use and misuse

of antimicrobial drugs accelerate the emergence of drug-resistant

microbes, which were further spread by poor sanitary conditions and

meager infection control [1]. Antimicrobial drugs prescribed in nearly

is a clinically signicant, urease-producing, Gram-negative Bacillus

and usually found in both water and land atmospheres. It is generally

associated with opportunistic infections in humans such as traveler's

diarrhea, urinary tract infections (UTI), skin infection, gastroenteritis,

infection is common throughout the world with 6-33% of mortality

rate, which is even greater in polymicrobial infection [2,3]. Recently,

P. rettgeri has acquired antimicrobial resistance due to producing

β-lactamase enzymes [4,5]. erefore, due to the clinical signicance

of P. rettgeri, development of eective antimicrobial therapy is very

needful for human health. As such, no medication is available to cure

the resistant strain of microbe but an alternative approach known

as bioeld treatment is recently reported to alter the antimicrobial

sensitivity in dierent microorganism [6].

e law of mass-energy inter-conversion is existed in the literature

for more than 300 years, and the thought was initially reported by

Hasenohrl followed by Einstein[7,8]. However, the conversion of mass

into energy is well established, but its inversion i.e., energy into mass

has not yet proven scientically. Furthermore, the energy can exists in

several forms such as kinetic, potential, electrical, magnetic, and nuclear.

Similarly, the human nervous system consists the energy in the form

of electrical signals [9,10]. us, human has the ability to harness the

energy from environment or universe and can transmit into any leaving

or nonliving object(s) around the Globe. e objects always receive the

energy and responding into useful way that is called bioeld energy and

the process is known as bioeld treatment. Whenever these electrical

signals uctuate with time, the magnetic eld generates as per the

Ampere-Maxwell law, and cumulatively known as electromagnetic eld.

Hence, the electromagnetic eld being generated from the human body

is known as bioeld [11]. Mr. Mahendra Trivedi's bioeld treatment has

shown to transform the characteristics non-living and living things in

several elds such as material science [12-17], agriculture [18-20], and

biotechnology [21,22]. e bioeld treatment has considerably altered

the sensitivity of antimicrobials to some microbes [6,23,24].

By conceiving the challenges of antimicrobial resistance in P.

rettgeri, and advantages of bioeld treatment; this work was undertaken

to evaluate the eects of bioeld treatment on antimicrobials sensitivity,

biotype number based on various biochemical reactions, and 16S rDNA

gene sequencing of P. rettgeri.

e sample vial of P. rettgeri [American Type Culture Collection

(ATCC) 9250] was procured from MicroBioLogics, Inc., USA, and

Volume 4 * Issue 2 * 1000129

ISSN: 2168-9547 MBL, an open access journal

Citation: Trivedi MK, Patil S, Shettigar H , Bairwa K, Jana S (2015) Effect of Bioeld Treatment on Phenotypic and Genotypic Characteristic of

stored as per the suggested storage conditions until further use. e

antimicrobial susceptibility, biochemical reactions, and biotype

number were evaluated on MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA) using Negative Breakpoint Combo 30

(NBPC30) panel. e 16S rDNA sequencing study was carried out in

Gr. III sampleusing Ultrapure Genomic DNA Prep Kit; Cat KT 83

(Bangalore Genei, India).

e samples of P. rettgeri was divided in three groups: Gr.I

(control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized).

Subsequently, the treatment groups (Gr. II and III) were received bioeld

treatment. e treatment groups were in sealed pack and handed over

to Mr. Trivedi for bioeld treatment under laboratory condition. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated groups without touching the samples. Treated samples

were assessed for antimicrobial sensitivity, biochemical reactions, and

biotyping of P. rettgeri. e assays for Gr.II were assessed on day 5 and

10, and Gr.III was assessed on day 10. e 16S rDNA gene sequencing

of P. rettgeri was also carried out.

Evaluation of antimicrobial susceptibility of P. rettgeri

out with the help of automated instrument, MicroScan Walk-

Away® using Negative Breakpoint Combo 30 (NBPC30) panel, as

per the manufacturer's instructions [25]. e minimum inhibitory

concentration (MIC) and a qualitative susceptibility like resistant

(R), intermediate (I), susceptible (S), or inducible β-lactamases (IB)

were determined by observing the lowest antimicrobial concentration

showing growth inhibition [26]. e antimicrobial sensitivity study was

carried out using following antimicrobials like amikacin, amoxicillin/

K-clavulanate, ampicillin/sulbactam, ampicillin, aztreonam,

cefazolin, cefepime, cefotaxime, cefotetan, cefoxitin, ceazidime,

cefuroxime, ceriaxone, cephalothin, chloramphenicol, ciprooxacin,

gatioxacin, gentamicin, imipenem, levooxacin, meropenem,

moxioxacin, nitrofurantoin, noroxacin, piperacillin, piperacillin/

tazobactam, tetracycline, ticarcillin, tobramycin, and trimethoprim/

sulfamethoxazole. All these antimicrobials were purchased from

e biochemical studies of P. rettgeri were performed on MicroScan

Walk-Away® [27,28]. Biochemical reactions patterns were carried out using

32 biochemicals viz. acetamide, adonitol, arabinose, arginine, cetrimide,

cephalothin, citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose,

hydrogen sulde, indole, inositol, kanamycin, lysine, malonate, melibiose,

nitrate, oxidation-fermentation media, galactosidase, ornithine, oxidase,

ranose, Rhamnose, sorbitol, sucrose, tartarate, tryptophan deaminase,

tobramycin, urea, and Voges-Proskauer. All these biochemical were

procured from Sigma-Aldrich, USA.

e biotype numbers of P. rettgeri was determined by automated

MicroScan Walk-Away® processed panel data utilizing biochemical

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated and puried from treated group of P.

rettgeri cells by using genomic purication Kit, as per the manufacturer's

instructions. e 16S rDNA gene (~1.5 kb) was amplied employing

universal primers forward 5'-AGAGTTTGATCCTGGC-3' and reverse

5'-GGTTACCTTGTTACGACTT-3'. Aer that, the amplied products

were subjected to gel electrophoresis on 1.0% agarose gel, stained with

ethidium bromide, and visualized under UV light in a gel documentation

unit (BioRad Laboratories, USA). e amplied fragment of PCR was

puried from the agarose gel by DNA Gel Extraction Kit. Sequencing of

amplied product was carried out on commercial basis from Bangalore

Genei, India. e obtained 16S rDNA sequences data were aligned

and compared with the sequences, available in Gene Bank database

of National Center for Biotechnology Information (NCBI) using

the algorithm BLASTn program. e multiple sequence alignment/

phylogenetic tree were constructed using MEGA 3.1 soware using

neighbour joining method [29].

Evaluation of antimicrobial susceptibility

pattern and MIC of tested antimicrobials are summarized in Table 1

alteration in susceptibility of 50% and 53.3% of total antimicrobials

in Gr.II on day 5 and 10, respectively; and alteration of 53.3% of total

antimicrobials in Gr.III on 10th day, with about 2-4 folds decrease in the

MIC values of respective antimicrobials. Briey, amikacin, cefepime,

chloramphenicol, gentamicin, and tobramycin were converted from

2Amoxicillin/K-clavulanate IB IB IB IB

3 Ampicillin/SulbactamI IB IB IB

11 Ceftazidime R IB IB IB

13 CeftriaxoneIB IB IB IB

14CephalothinR IB IB IB

15 Chloramphenicol R S S S

25PiperacillinIB IB IB IB

26 Piperacillin/Tazobactam IB IB IB IB

30 Trimethoprim/Sulfamethoxazole S S S S

Gr: Group; I: Intermediate; S: Susceptible; R: Resistant; IB: Reduced Activity of

of selected antimicrobials.

Volume 4 * Issue 2 * 1000129

ISSN: 2168-9547 MBL, an open access journal

Citation: Trivedi MK, Patil S, Shettigar H , Bairwa K, Jana S (2015) Effect of Bioeld Treatment on Phenotypic and Genotypic Characteristic of

resistant (control) to susceptible in treated groups (Gr.II and Gr.III in

all assessment). Similarly, cefotetan, cefoxitin, ceazidime, cefuroxime,

cefotaxime, cephalothin, and aztreonam were changed from resistant

to inducible β-lactamase in entire treated groups. e sensitivity of

ampicillin was altered from resistant to intermediate and inducible

β-lactamase in Gr.II and III, respectively on day 10. Further, the

ampicillin/sulbactam, cefazolin, and ticarcillin were converted from

intermediate to inducible β-lactamase in all the treated groups. e

MIC of all the above-mentioned antimicrobials were decreased about

2-folds except the ticarcillin and cefotaxime that showed about 4-folds

decrease in MIC value.

Organism identication by biochemical reactions

revealed an alteration in biochemical reaction pattern as 12.1% of total

biochemicals in Gr.II on day 5 and 10, and 48.5% of total biochemicals

in Gr.III on day 10. Briey, the cephalothin, kanamycin, and tobramycin

biochemical reactions were converted from positive to negative reaction

in entire treated groups (Gr.II on day 5 and 10 and Gr.III on day 10).

Biochemicals such as arabinose, hydrogen sulde, lysine, malonate,

melibiose, galactosidase, ornithine, ranose, Rhamnose, sorbitol,

sucrose, and Voges-Proskauer were changed from positive to negative

reaction only in Gr.III on day 10 with respect to control. Further,

tartarate was converted from negative to positive reaction in Gr.II on

day 5 only, and tryptophan was converted from negative to positive in

Gr.II and Gr.III on day 10, as compared to control.

Eect of bioeld treatment on biotype number

e biotype numbers of P. rettgeri was determined on MicroScan

Walk-Away® processed panel, using biochemical reaction data. e

treated groups (on all assessment day) as compared to control (Table 4).

e 16S rDNA sequence was determined in P. rettgeri. e

1Amikacin>32 ≤16 ≤16 ≤16

3 Ampicillin/Sulbactam16/8 ≤8/4 ≤8/4 ≤8/4

9Cefotetan>32 ≤16 ≤16 ≤16

13Ceftriaxone≤8 ≤8 ≤8 ≤8

16 Ciprooxacin≤1 ≤1 ≤1 ≤1

17Gatioxacin≤2 ≤2 ≤2 ≤2

20 Levooxacin≤2 ≤2 ≤2 ≤2

22 Moxioxacin≤2 ≤2 ≤2 ≤2

23 Nitrofurantoin>64 >64 >64 >64

24 Noroxacin≤4 ≤4 ≤4 ≤4

25 Piperacillin≤16 ≤16 ≤16 ≤16

26 Piperacillin/Tazobactam ≤16 ≤16 ≤16 ≤16

27 Tetracycline>8 >8 >8 >8

Sulfamethoxazole ≤2/38 ≤2/38 ≤2/38 ≤2/38

Gr: Group; MIC data are presented in µg/mL; ESBL-a, b Scrn: Extended-Spectrum

Table 2: Effect of bioeld treatment on minimum inhibitory concentration (MIC) of

Gr: Group; - (Negative); + (Positive)

# data[].attributes.attachment_link.title
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri - PdfSR.com

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
Antimicrobial Susceptibility of Provindencia rettgeriThe study was undertaken to evaluate the impact of human energy treatment on the P. rettgeri antimicrobial susceptibility pattern of treated sample as compared to the untreated sample.

# data[].attributes.subject
Antimicrobial Susceptibility of Provindencia rettgeriThe study was undertaken to evaluate the impact of human energy treatment on the P. rettgeri antimicrobial susceptibility pattern of treated sample as compared to the untreated sample.

# data[].attributes.attachment_link.title
"Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
Human Energy Treatment on P. rettgeriP. rettgeri was commonly associated with the hospital-acquired infections. The present research was focused on the concept of energy treatment & its impact on the characteristics of Providencia Rettgeri. Follow the link for more information.

# data[].attributes.subject
Human Energy Treatment on P. rettgeriP. rettgeri was commonly associated with the hospital-acquired infections. The present research was focused on the concept of energy treatment & its impact on the characteristics of Providencia Rettgeri. Follow the link for more information.

# data[].attributes.attachment_link.keywords[]
biofield - biofield treatment - mahendra kumar trivedi - mahendra trivedi - trivedi effect - antimicrobial susceptibility - providencia rettgeri

# data[].attributes.attachment_link.title
Effect of Biofield Treatment on Phenotypic and Genotypic Chara...

# data[].attributes.attachment_link.description
Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.

# data[].attributes.headline
Minimum Inhibitory Concentration in Treated P. rettgeriMIC results showed a significant decrease in various antimicrobials of P. rettgeri that was due to the impact from the unique alternative energy treatment.

# data[].attributes.subject
Minimum Inhibitory Concentration in Treated P. rettgeriMIC results showed a significant decrease in various antimicrobials of P. rettgeri that was due to the impact from the unique alternative energy treatment.

# data[].attributes.headline
P. rettgeri | 16S rDNA SequencingThe 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of treated P. rettgeri with other bacterial species. Follow the link, to know more.

# data[].attributes.subject
P. rettgeri | 16S rDNA SequencingThe 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of treated P. rettgeri with other bacterial species. Follow the link, to know more.

# data[].attributes.headline
Alteration in Biotype Number of P. rettgeriIn the current research, the result exhibited alteration in biotype number of P. rettgeri in the entire treated groups as compared to control. Follow the link for more details.

# data[].attributes.subject
Alteration in Biotype Number of P. rettgeriIn the current research, the result exhibited alteration in biotype number of P. rettgeri in the entire treated groups as compared to control. Follow the link for more details.

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Evaluation of Provindencia Rettgeri Biochemical ReactionsThe study results shows alterations in the biochemical reactions pattern of Provindencia Rettgeri after the influence of a unique human energy treatment. Follow the link for more details.

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Evaluation of Provindencia Rettgeri Biochemical ReactionsThe study results shows alterations in the biochemical reactions pattern of Provindencia Rettgeri after the influence of a unique human energy treatment. Follow the link for more details.

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Biofield Treatment on P. rettgeriPresent study was designed to evaluate the effect of biofield treatment on P. rettgeri against the antimicrobial susceptibility and other biochemical characteristics. Visit the link for more details.

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Biofield Treatment on P. rettgeriPresent study was designed to evaluate the effect of biofield treatment on P. rettgeri against the antimicrobial susceptibility and other biochemical characteristics. Visit the link for more details.

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Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri
Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Khemraj Bairwa2 and Snehasis Jana2*
¹ Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

² Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

*Corresponding Author:

Dr. Snehasis Jana
Trivedi Science Research Laboratory Pvt. Ltd.
Hall-A, Chinar Mega Mall, Chinar Fortune City
Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India
Tel: +91-755-6660006
E-mail: [email protected]

Received date: July 03, 2015; Accepted date: July 20, 2015; Published date: July 27, 2015

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri. Mol Biol 4:129. doi:10.4172/2168-9547.1000129

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence. The samples of P. rettgeri (ATCC 9250) were divided into three groups: Gr.I (control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized). The Gr.II and III were treated with Mr. Trivedi's biofield, and then subsequently characterized for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype numbering. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. rettgeri with other bacterial species. The treated cells of P. rettgeri showed an alteration in susceptibility of about 50% and 53.3% tested antimicrobials of Gr.II on day 5 and 10, respectively; and 53.3% of tested antimicrobials of Gr.III on day 10. MIC results showed a significant decrease in MIC values of 53.1, 56.3, and 56.3% antimicrobials in Gr.II on day 5, Gr.II on day 10, and Gr.III on day 10, respectively, as compared to control. The significant changes in biochemical reactions and biotype numbers were also observed in all the treated groups of P. rettgeri. Based on nucleotides homology and phylogenetic analysis the P. rettgeri was found to be Proteus mirabilis (GenBank Accession Number: AY820623) and nearest homolog species was found to be Proteus vulgaris (Accession No. DQ499636). These findings suggest that biofield treatment can prevent the emergence of absolute resistance of existing antimicrobials to P. rettgeri.

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providencia rettgeri; biofield treatment; antimicrobial susceptibility; biotype; 16s rdna sequencing

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Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri

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Mahendra Kumar Trivedi1 , Shrikant Patil1 , Harish Shettigar1 , Khemraj Bairwa2 and Snehasis Jana2 * 1 Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA 2 Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India *Corresponding Author: Dr. Snehasis Jana Trivedi Science Research Laboratory Pvt.