# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.readable_text
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구 : Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice

Abstract

Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines. Particularly, embryonic stem-cell gene-targeting method allowed researchers to generate model mice having a gene knockout or mutation in a gene of interest. Furthermore, this approach enabled us to evaluate the relationships among different phenomena observed at molecular, cellular, and systems levels. On the other hand, since many pioneering neuroscientists such as Cajal and Hebb proposed the possible principle of information storage in the nervous system, lots of efforts have been made to search for the molecular and genetic basis for learning and memory in neuroscience.
In this context, the genetic approaches were early introduced into neuroscience field and contributed heavily to the advancement of our understanding for molecular mechanisms of learning and memory. Especially, transgenetic mice model have greatly expanded the knowledge about how the events in molecular and cellular level could induce the alteration of neuronal circuit, subsequently leading to the behavioral output. Here, using the two gene deletion (knockout) mice lines, I investigated the unknown role of two enzymes, PI3Kγ and CD38 in the brain. In particular, the physiological functions of these two proteins are evaluated utilizing both electrophysiological and behavioral approaches.
Phosphatidylinositol 3-kinase (PI3K) has been reported to be involved in key cellular processes such as cell survival, proliferation, migration, and adhesion. In addition, a growing body of evidence has revealed the role of this kinase as a capital player in synaptic plasticity and neural functions in brain. Nevertheless, the origin of functional diversity of PI3K in brain is elusive and less understood. In this study, I investigated the role of PI3Kγ, the only member of class IB PI3K, in hippocampal-dependent synaptic plasticity and cognitive functions. I found that PI3Kγ existed in hippocampus and played a crucial role in N-methyl-D-aspartate (NMDA) receptor-dependent long-term depression (LTD) at Schaffer Collateral-Cornu Ammonis (SC-CA1) synapses. Both genetic deletion and pharmacological inhibition of PI3Kγ disrupted NMDAR-LTD, while leaving other forms of synaptic plasticity intact. Accompanying this physiological deficit, the activation of p38 mitogen-activated protein kinase (MAPK) was impaired in parallel in PI3Kγ KO mice. Furthermore, the impairment of NMDAR-LTD by PI3Kγ blockade was specifically correlated with deficits in behavioral flexibility. These findings suggest that a specific PI3K isoform, PI3Kγ, is critical for NMDAR-LTD as well as behavioral flexibility.
CD38 is a multifunctional enzyme, mediating the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD to ADP-ribose. It has been shown that CD38 is implicated in a wide range of cellular processes such as cell cycle regulation, gene expression, insulin secretion, and transmitter release via Ca2+ signaling pathway. In addition, a recent paper has revealed the interesting role of CD38 as a molecule important for social behavior and maternal nurturing. However, the role of CD38 in hippocampal-dependent synaptic plasticity and behavior remains largely unknown. In this study, I found that adult CD38 KO mice exhibited the deficit in L-LTP maintenance at hippocampal SC-CA1 synapses. Moreover, this physiological deficit was accompanied by the impairment in novel object recognition task. These results demonstrate that CD38 is a key enzyme for L-LTP maintenance at hippocampal SC-CA1 synapses, thereby mediating specify hippocampal-dependent memory formation.
In summary, I found that the both class IB PI3Kγ and CD38 mediated the critical function in hippocampal synaptic plasticity and cognitive behaviors such as behavioral flexibility and memory formation. These data strongly propose that the transgenetic mice model is a powerful tool to dissect out the physiological functions of specific genes at molecular, cellular, and systems levels.
신경생물학자들의 연구 목표는 신경세포 수준의 변화와 동물 행동 변화 사이의 직접적인 상관관계를 밝혀내는 것이며, 더 나아가 이들 사이의 인과관계를 이끌어 내어 동물들의 다양한 행동들을 만들어 내는 분자, 세포학적 기전을 규명하는 것이다. 유전학적인 방법들은 이러한 목표를 달성하는데 지금껏 큰 기여를 하였으며, 특히 다양한 유전자 조작 생쥐 모델들을 대상으로 시냅스 가소성이나 학습과 기억의 바탕이 되는 분자 메커니즘이 연구되어 왔다.
유전자 조작 생쥐 모델을 이용한 대표적인 연구 성과로는 NMDA 수용체가 해마 의존적인 시냅스 가소성과 학습, 그리고 기억과 같은 고등 인지기능에 핵심적인 역할을 하며, NMDA 수용체를 통한 활성 신호는 인산화 효소 αCaMKII로 연결되어 해마의 생리학적 기능들을 조절한다는 것이다. 또한, 또 다른 인산화 효소 PKA도 NMDA 수용체 등을 통해 활성화되어 장기 시냅스 가소성 및 장기 기억의 형성에 중요한 역할을 할 가능성이 제기되었다.
앞선 연구들을 통해 여러 유전자들이 해마의 시냅스 가소성과 학습, 그리고 기억 현상에 중요한 역할을 한다는 사실들이 알려졌지만, 여전히 많은 유전자들의 뇌에서의 기능은 알려져 있지 않다. 또한, 시냅스 가소성 및 고등 인지기능에 관여하는 분자, 세포학적 메커니즘의 이해를 위해서는 이렇게 아직 까지 연구되어 있지 않은 유전자들의 구체적인 기능 연구와 이들이 다양한 상황에서 서로간에 어떻게 상호작용 하는지에 대한 통합적 접근이 필요하다.
이러한 맥락에서, 본 연구에서는 해마에서의 기능 연구가 전무한 PI3K class IB isoform, PI3K 감마, 그리고 이와 유사하게 다기능 효소인 CD38을 대상으로 해마 의존적인 시냅스 가소성과 인지 기능에 미치는 영향에 대한 실험들을 진행하였다. PI3K가 뇌에서 가지는 기능들의 경우 다양한 isoform의 특이적 기능들에 초점을 맞춘 연구는 전무하였는데, 본 연구를 통해서 신경세포와 심근세포, 조혈모세포, 그리고 면역세포 일부에만 발현되는 것으로 알려진 class IB PI3K 감마가 해마 시냅스에서의 장기 시냅스 저하 현상에 특이적으로 관여하다는 사실을 밝혀내었다. 또한, 이러한 해마 신경회로에서의 생리학적 변화는 동물 행동 유연성의 변화를 이끌어 내는 것으로 나타났다. 또한, 해마에서의 기능 연구가 전무한 CD38 유전자의 경우, SC-CA1 시냅스에서의 장기 시냅스 강화와 해마 의존적 장기 기억 형성에 중요한 역할을 할 가능성을 확인하였다.
종합하면, 이러한 연구들을 통해서 PI3K 감마와 CD38이 해마 의존적인 시냅스 가소성과 동물 인지행동에 중요한 역할을 할 가능성을 확인하였다. 또한, 신경과학 분야에서 그 기능이 연구되지 않은 많은 유전자들이 다양한 종류의 신경 생리 및 동물 행동에 다양한 방식으로 관여할 가능성을 시사하고 있다.

URI

https://hdl.handle.net/10371/156396

http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000000618

Files in This Item: There are no files associated with this item.

Appears in Collections:

College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Ph.D. / Sc.D._생명과학부)

# data[].attributes.attachment_link.title
SNU Open Repository and Archive: PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구

# data[].attributes.attachment_link.description
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구 : Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice Abstract Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines.

# data[].attributes.body
2. 구글 스칼라
https://academic.oup.com/hmg/article/14/suppl_1/R41/560902?login=false

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.title
醫部全錄의 編輯體制와 主題分類 (1)

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
https://link.springer.com/article/10.1007/s000180050314
https://pubmed.ncbi.nlm.nih.gov/19424149/

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.headline
기억 유전자 관련 논문 

# data[].attributes.subject
기억 유전자 관련 논문 

# data[].attributes.body
구글 스칼라: https://kmbase.medric.or.kr/KMID/1123920020160051025

pubmed : https://pubmed.ncbi.nlm.nih.gov/26280931/

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.title
초등학생의 비만유발유전자 다형에 따른 신체구성, 뇌신경세포 성장인자와 기억력과의 관계

# data[].attributes.attachment_link.readable_text
The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Free article

Free article

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storage, and have avoided the question of how individual memories are stored in the brain. Memory engram technology allows the labeling and subsequent manipulation of components of specific memory engrams in particular brain regions, and it has been established that cell ensembles labeled by this method are both sufficient and necessary for memory recall. Recent research has employed this technology to probe fundamental questions of memory consolidation, differentiating between mechanisms of memory retrieval from the true neurobiology of memory storage.

Similar articles

Synapse-specific representation of the identity of overlapping memory engrams.

Abdou K, Shehata M, Choko K, Nishizono H, Matsuo M, Muramatsu SI, Inokuchi K. Abdou K, et al. Science. 2018 Jun 15;360(6394):1227-1231. doi: 10.1126/science.aat3810. Science. 2018. PMID: 29903972

Cited by

Enhancing Immediate Memory, Potential Learning, and Working Memory with Transcranial Direct Current Stimulation in Healthy Older Adults.

Satorres E, Meléndez JC, Pitarque A, Real E, Abella M, Escudero J. Satorres E, et al. Int J Environ Res Public Health. 2022 Oct 5;19(19):12716. doi: 10.3390/ijerph191912716. Int J Environ Res Public Health. 2022. PMID: 36232016 Free PMC article. Clinical Trial.

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Memory engram storage and retrieval - PubMed

# data[].attributes.attachment_link.description
A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storag ...

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
(DBPia로 검색한 결과)

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.readable_text
Affiliation

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Free PMC article

Free PMC article

Affiliation

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

A specific memory is thought to be encoded by a sparse population of neurons. These neurons can be tagged during learning for subsequent identification and manipulation. Moreover, their ablation or inactivation results in reduced memory expression, suggesting their necessity in mnemonic processes. However, the question of sufficiency remains: it is unclear whether it is possible to elicit the behavioural output of a specific memory by directly activating a population of neurons that was active during learning. Here we show in mice that optogenetic reactivation of hippocampal neurons activated during fear conditioning is sufficient to induce freezing behaviour. We labelled a population of hippocampal dentate gyrus neurons activated during fear learning with channelrhodopsin-2 (ChR2) and later optically reactivated these neurons in a different context. The mice showed increased freezing only upon light stimulation, indicating light-induced fear memory recall. This freezing was not detected in non-fear-conditioned mice expressing ChR2 in a similar proportion of cells, nor in fear-conditioned mice with cells labelled by enhanced yellow fluorescent protein instead of ChR2. Finally, activation of cells labelled in a context not associated with fear did not evoke freezing in mice that were previously fear conditioned in a different context, suggesting that light-induced fear memory recall is context specific. Together, our findings indicate that activating a sparse but specific ensemble of hippocampal neurons that contribute to a memory engram is sufficient for the recall of that memory. Moreover, our experimental approach offers a general method of mapping cellular populations bearing memory engrams.

Similar articles

Inception of a false memory by optogenetic manipulation of a hippocampal memory engram.

Liu X, Ramirez S, Tonegawa S. Liu X, et al. Philos Trans R Soc Lond B Biol Sci. 2013 Dec 2;369(1633):20130142. doi: 10.1098/rstb.2013.0142. Print 2014 Jan 5. Philos Trans R Soc Lond B Biol Sci. 2013. PMID: 24298144 Free PMC article. Review.

Effects of whole body (56)Fe radiation on contextual freezing and Arc-positive cells in the dentate gyrus.

Raber J, Allen AR, Rosi S, Sharma S, Dayger C, Davis MJ, Fike JR. Raber J, et al. Behav Brain Res. 2013 Jun 1;246:162-7. doi: 10.1016/j.bbr.2013.02.022. Epub 2013 Feb 28. Behav Brain Res. 2013. PMID: 23454674

Creating a false memory in the hippocampus.

Ramirez S, Liu X, Lin PA, Suh J, Pignatelli M, Redondo RL, Ryan TJ, Tonegawa S. Ramirez S, et al. Science. 2013 Jul 26;341(6144):387-91. doi: 10.1126/science.1239073. Science. 2013. PMID: 23888038

Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model.

Iwakura Y, Kawahara-Miki R, Kida S, Sotoyama H, Gabdulkhaev R, Takahashi H, Kunii Y, Hino M, Nagaoka A, Izumi R, Shishido R, Someya T, Yabe H, Kakita A, Nawa H. Iwakura Y, et al. Neurochem Res. 2022 Apr 25. doi: 10.1007/s11064-022-03599-9. Online ahead of print. Neurochem Res. 2022. PMID: 35469366

The Neurobiology of Behavior and Its Applicability for Animal Welfare: A Review.

Coria-Avila GA, Pfaus JG, Orihuela A, Domínguez-Oliva A, José-Pérez N, Hernández LA, Mota-Rojas D. Coria-Avila GA, et al. Animals (Basel). 2022 Apr 4;12(7):928. doi: 10.3390/ani12070928. Animals (Basel). 2022. PMID: 35405916 Free PMC article. Review.

Neuronal Ensembles Organize Activity to Generate Contextual Memory.

Marks WD, Yokose J, Kitamura T, Ogawa SK. Marks WD, et al. Front Behav Neurosci. 2022 Mar 15;16:805132. doi: 10.3389/fnbeh.2022.805132. eCollection 2022. Front Behav Neurosci. 2022. PMID: 35368306 Free PMC article. Review.

References

Josselyn SA. Continuing the search for the engram: examining the mechanism of fear memories. J Psychiatry Neurosci. 2010;35:221-228. - PMC - PubMed

Silva AJ, et al. Molecular and cellular approaches to memory allocation in neural circuits. Science. 2009;326:391-395. - PMC - PubMed

Reijmers LG, Perkins BL, Matsuo N, Mayford M. Localization of a stable neural correlate of associative memory. Science. 2007;317:1230-1233. - PubMed

Han JH, et al. Neuronal competition and selection during memory formation. Science. 2007;316:457-460. - PubMed

Han JH, et al. Selective erasure of a fear memory. Science. 2009;323:1492-1496. - PubMed

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Optogenetic stimulation of a hippocampal engram activates fear memory recall - PubMed

# data[].attributes.attachment_link.description
A specific memory is thought to be encoded by a sparse population of neurons. These neurons can be tagged during learning for subsequent identification and manipulation. Moreover, their ablation or inactivation results in reduced memory expression, suggesting their necessity in mnemonic processes. H ...

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향 (1)

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.readable_text
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구
Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice

Abstract

Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines. Particularly, embryonic stem-cell gene-targeting method allowed researchers to generate model mice having a gene knockout or mutation in a gene of interest. Furthermore, this approach enabled us to evaluate the relationships among different phenomena observed at molecular, cellular, and systems levels. On the other hand, since many pioneering neuroscientists such as Cajal and Hebb proposed the possible principle of information storage in the nervous system, lots of efforts have been made to search for the molecular and genetic basis for learning and memory in neuroscience.
In this context, the genetic approaches were early introduced into neuroscience field and contributed heavily to the advancement of our understanding for molecular mechanisms of learning and memory. Especially, transgenetic mice model have greatly expanded the knowledge about how the events in molecular and cellular level could induce the alteration of neuronal circuit, subsequently leading to the behavioral output. Here, using the two gene deletion (knockout) mice lines, I investigated the unknown role of two enzymes, PI3Kγ and CD38 in the brain. In particular, the physiological functions of these two proteins are evaluated utilizing both electrophysiological and behavioral approaches.
Phosphatidylinositol 3-kinase (PI3K) has been reported to be involved in key cellular processes such as cell survival, proliferation, migration, and adhesion. In addition, a growing body of evidence has revealed the role of this kinase as a capital player in synaptic plasticity and neural functions in brain. Nevertheless, the origin of functional diversity of PI3K in brain is elusive and less understood. In this study, I investigated the role of PI3Kγ, the only member of class IB PI3K, in hippocampal-dependent synaptic plasticity and cognitive functions. I found that PI3Kγ existed in hippocampus and played a crucial role in N-methyl-D-aspartate (NMDA) receptor-dependent long-term depression (LTD) at Schaffer Collateral-Cornu Ammonis (SC-CA1) synapses. Both genetic deletion and pharmacological inhibition of PI3Kγ disrupted NMDAR-LTD, while leaving other forms of synaptic plasticity intact. Accompanying this physiological deficit, the activation of p38 mitogen-activated protein kinase (MAPK) was impaired in parallel in PI3Kγ KO mice. Furthermore, the impairment of NMDAR-LTD by PI3Kγ blockade was specifically correlated with deficits in behavioral flexibility. These findings suggest that a specific PI3K isoform, PI3Kγ, is critical for NMDAR-LTD as well as behavioral flexibility.
CD38 is a multifunctional enzyme, mediating the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD to ADP-ribose. It has been shown that CD38 is implicated in a wide range of cellular processes such as cell cycle regulation, gene expression, insulin secretion, and transmitter release via Ca2+ signaling pathway. In addition, a recent paper has revealed the interesting role of CD38 as a molecule important for social behavior and maternal nurturing. However, the role of CD38 in hippocampal-dependent synaptic plasticity and behavior remains largely unknown. In this study, I found that adult CD38 KO mice exhibited the deficit in L-LTP maintenance at hippocampal SC-CA1 synapses. Moreover, this physiological deficit was accompanied by the impairment in novel object recognition task. These results demonstrate that CD38 is a key enzyme for L-LTP maintenance at hippocampal SC-CA1 synapses, thereby mediating specify hippocampal-dependent memory formation.
In summary, I found that the both class IB PI3Kγ and CD38 mediated the critical function in hippocampal synaptic plasticity and cognitive behaviors such as behavioral flexibility and memory formation. These data strongly propose that the transgenetic mice model is a powerful tool to dissect out the physiological functions of specific genes at molecular, cellular, and systems levels.
신경생물학자들의 연구 목표는 신경세포 수준의 변화와 동물 행동 변화 사이의 직접적인 상관관계를 밝혀내는 것이며, 더 나아가 이들 사이의 인과관계를 이끌어 내어 동물들의 다양한 행동들을 만들어 내는 분자, 세포학적 기전을 규명하는 것이다. 유전학적인 방법들은 이러한 목표를 달성하는데 지금껏 큰 기여를 하였으며, 특히 다양한 유전자 조작 생쥐 모델들을 대상으로 시냅스 가소성이나 학습과 기억의 바탕이 되는 분자 메커니즘이 연구되어 왔다.
유전자 조작 생쥐 모델을 이용한 대표적인 연구 성과로는 NMDA 수용체가 해마 의존적인 시냅스 가소성과 학습, 그리고 기억과 같은 고등 인지기능에 핵심적인 역할을 하며, NMDA 수용체를 통한 활성 신호는 인산화 효소 αCaMKII로 연결되어 해마의 생리학적 기능들을 조절한다는 것이다. 또한, 또 다른 인산화 효소 PKA도 NMDA 수용체 등을 통해 활성화되어 장기 시냅스 가소성 및 장기 기억의 형성에 중요한 역할을 할 가능성이 제기되었다.
앞선 연구들을 통해 여러 유전자들이 해마의 시냅스 가소성과 학습, 그리고 기억 현상에 중요한 역할을 한다는 사실들이 알려졌지만, 여전히 많은 유전자들의 뇌에서의 기능은 알려져 있지 않다. 또한, 시냅스 가소성 및 고등 인지기능에 관여하는 분자, 세포학적 메커니즘의 이해를 위해서는 이렇게 아직 까지 연구되어 있지 않은 유전자들의 구체적인 기능 연구와 이들이 다양한 상황에서 서로간에 어떻게 상호작용 하는지에 대한 통합적 접근이 필요하다.
이러한 맥락에서, 본 연구에서는 해마에서의 기능 연구가 전무한 PI3K class IB isoform, PI3K 감마, 그리고 이와 유사하게 다기능 효소인 CD38을 대상으로 해마 의존적인 시냅스 가소성과 인지 기능에 미치는 영향에 대한 실험들을 진행하였다. PI3K가 뇌에서 가지는 기능들의 경우 다양한 isoform의 특이적 기능들에 초점을 맞춘 연구는 전무하였는데, 본 연구를 통해서 신경세포와 심근세포, 조혈모세포, 그리고 면역세포 일부에만 발현되는 것으로 알려진 class IB PI3K 감마가 해마 시냅스에서의 장기 시냅스 저하 현상에 특이적으로 관여하다는 사실을 밝혀내었다. 또한, 이러한 해마 신경회로에서의 생리학적 변화는 동물 행동 유연성의 변화를 이끌어 내는 것으로 나타났다. 또한, 해마에서의 기능 연구가 전무한 CD38 유전자의 경우, SC-CA1 시냅스에서의 장기 시냅스 강화와 해마 의존적 장기 기억 형성에 중요한 역할을 할 가능성을 확인하였다.
종합하면, 이러한 연구들을 통해서 PI3K 감마와 CD38이 해마 의존적인 시냅스 가소성과 동물 인지행동에 중요한 역할을 할 가능성을 확인하였다. 또한, 신경과학 분야에서 그 기능이 연구되지 않은 많은 유전자들이 다양한 종류의 신경 생리 및 동물 행동에 다양한 방식으로 관여할 가능성을 시사하고 있다.

URI

https://hdl.handle.net/10371/156396

http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000000618

Files in This Item: There are no files associated with this item.

Appears in Collections:

College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Ph.D. / Sc.D._생명과학부)

# data[].attributes.attachment_link.title
SNU Open Repository and Archive: PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구

# data[].attributes.attachment_link.description
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구 Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice Abstract Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines.

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
https://pubmed.ncbi.nlm.nih.gov/10518471/

https://www.sciencedirect.com/science/article/pii/S0092867412004680

# data[].attributes.body
혹스 유전자 - Ubx
GenBank: LT986705

Metrobates hesperius mRNA for UBX (Ubx gene)

        1 cgccaccaca ccccaccacc cgccacccca tccttacgtc acgatattac tatttttata
       61 gctccataaa atttttatac ccgacccttc cgttttatat cgtcaagtac cttttgaatg
      121 cggtcgttcc ccaactgatt tcaaaccagc aaaaattttt cggatcattt ttaaaaaaat
      181 ttgatgctgt aatcgtcgtc gcggattccg gcagtcggcg tgccggctat cgagatgaac
      241 tcgtacttcg agcagacggg tttttacggg gcgcatcatc accagggagg ttccggacat
      301 catcatcacg agcaggcagc cgcctacaga tttcccttgg gacttggcat gtcgccttac
      361 gcctccagtc aacaccatca tcacggtctt caggctccca gacctcagga ctcgccttac
      421 gacgcttcgg tcgccgccgc ctgcaaactt tattctgcct cagcggactc agccgcctcc
      481 aattacggct ctcccgttgg caaacccgat tgtagcaaga ccgagggtca caccaacggc
      541 tacggcaagg aagtgtgggg ccgcgaagcc ggaggcagat atggaggctt agccgggtcg
      601 ccggagagtg gtcggtcctc agcccaacct ttgggggcgg ccggtacccc agctccggcg
      661 tggaaccagt gcagcatcac tcccgcttcc ggacagccgg cagtggcgcc ttcgccctta
      721 caccagcagg cctcccagca cacgttctac ccctggatgg ctatagcagg tactgctctt
      781 cattaaatga ctatataaag ccctccccat tgatttagct cttacggcac cctccttttg
      841 cactcccccg tttacactta ttaagctctt cggagcgtag tgccctactg accttcctat
      901 ttcggttgcg attcgtgcac ttgacaaccg tgaaatatg
//

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4858

https://pubmed.ncbi.nlm.nih.gov/23888038/

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
http://www.aistudy.com/paper/pdf/memory_kang.pdf

# data[].attributes.attachment_link.title
CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment

# data[].attributes.attachment_link.description
A key feature of brain plasticity is the experience-dependent selection of optimal connections· implemented by a set of activity-regulated genes that dynamically adjust synapse strength and number. The activity-regulated gene cpg15/neuritin has ...

# data[].attributes.body
https://pubmed.ncbi.nlm.nih.gov/26280931/

pubmed

# data[].attributes.attachment_link.readable_text
The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Free article

Free article

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storage, and have avoided the question of how individual memories are stored in the brain. Memory engram technology allows the labeling and subsequent manipulation of components of specific memory engrams in particular brain regions, and it has been established that cell ensembles labeled by this method are both sufficient and necessary for memory recall. Recent research has employed this technology to probe fundamental questions of memory consolidation, differentiating between mechanisms of memory retrieval from the true neurobiology of memory storage.

Similar articles

Synapse-specific representation of the identity of overlapping memory engrams.

Abdou K, Shehata M, Choko K, Nishizono H, Matsuo M, Muramatsu SI, Inokuchi K. Abdou K, et al. Science. 2018 Jun 15;360(6394):1227-1231. doi: 10.1126/science.aat3810. Science. 2018. PMID: 29903972

Cited by

Enhancing Immediate Memory, Potential Learning, and Working Memory with Transcranial Direct Current Stimulation in Healthy Older Adults.

Satorres E, Meléndez JC, Pitarque A, Real E, Abella M, Escudero J. Satorres E, et al. Int J Environ Res Public Health. 2022 Oct 5;19(19):12716. doi: 10.3390/ijerph191912716. Int J Environ Res Public Health. 2022. PMID: 36232016 Free PMC article. Clinical Trial.

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Memory engram storage and retrieval - PubMed

# data[].attributes.attachment_link.description
A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storag ...

# data[].attributes.headline
1. 기억유전자 관련 논문 (1) 구글 스칼라

# data[].attributes.subject
1. 기억유전자 관련 논문 (1) 구글 스칼라

# data[].attributes.body
Genbank accession number: KJ905799

FASTA:

GTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGA
CTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCGCCCCCAGGTTCACCTGGTGCAG
TCTGGAGCCGAGGTGAAGAAGCCTGGGGCCTCAGTTAAGCTTTCCTGCAAGACTTCTGGTTACAACTTTT
CCAGCTATGATCTCATATGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGC
TCATAATGGGGACACAAAGTATGCACGGAAGTTCCAGGGAAGAGTCACCATGACCACAGACACTTCCGCC
ACCACATCCTACATGGAGTTTAGGAGCCTGAGATCTGACGACACGGCCCTTTTTTACTGTGCGACTAAAT
CCCGCGGGCAGGTCGGCGACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
CAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCAGCCCTGGGC
TGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCG
TGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTC
CAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAG
AAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGG
GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCAC
ATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAG
GTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCA
CCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGC
CCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCA
TCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACA
TCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTC
CGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTC
TCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACACAGAAGAGCCTCTCCCTGTCTCCGGGTA
AATNNCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAAC

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.readable_text
1: Tonegawa S, Pignatelli M, Roy DS, Ryan TJ. Memory engram storage and
retrieval. Curr Opin Neurobiol. 2015 Dec;35:101-9. doi:
10.1016/j.conb.2015.07.009. Epub 2015 Aug 14. PMID: 26280931.

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
"기억 유전자" 또는 "remember gene"에 관한 논문을 검색

1) PubMed
2) 구글 스칼라
3) DBPia

→파일 전문 다운로드 or 불가능한 경우 링크
→패들렛에 업로드

# data[].attributes.body
암, 돌연변이 억제 p53 단백질 유전자
gene accession number: U94788.1

# data[].attributes.body
(1) GeneBank accession number: AY892288.1
(2) FASTA:

ATGAGTGAAGAGGAGCAAGGCTCCGGCACTACCACGGGCTGCGGGCTGCCTAGTATAGAGCAAATGCTGG
CCGCCAACCCAGGCAAGACCCCGATCAGCCTTCTGCAGGAGTATGGGACCAGAATAGGGAAGACGCCTGT
GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCACCAGCCTAATTTCACCTTCCGGGTCACCGTTGGCGAC
ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC
ACCTCAAAGGGGGGAGCATGCTGGAGCCGGCCCTGGAGGACAGCAGTTCTTTTTCTCCCCTAGACTCTTC
ACTGCCTGAGGACATTCCGGTTTTTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTGTAGTCCTAACC
AGGAGCCCCCCCATGGAACTGCAGCCCCCTGTCTCCCCTCAGCAGTCTGAGTGCAACCCCGTTGGTGCTC
TGCAGGAGCTGGTGGTGCAGAAAGGCTGGCGGTTGCCGGAGTACACAGTGACCCAGGAGTCTGGGCCAGC
CCACCGCAAAGAATTCACCATGACCTGTCGAGTGGAGCGTTTCATTGAGATTGGGAGTGGCACTTCCAAA
AAATTGGCAAAGCGGAATGCGGCGGCCAAAATGCTGCTTCGAGTGCACACGGTGCCTCTGGATGCCCGGG
ATGGCAATGAGGTGGAGCCTGATGATGACCACTTCTCCATTGGTGTGGGCTCCCGCCTGGATGGTCTTCG
AAACCGGGGCCCAGGTTGCACCTGGGATTCTCTACGAAATTCAGTAGGAGAGAAGATCCTGTCCCTCCGC
AGTTGCTCCCTGGGCTCCCTGGGTGCCCTGGGCCCTGCCTGCTGCCGTGTCCTCAGTGAGCTCTCTGAGG
AGCAGGCCTTTCACGTCAGCTACCTGGATATTGAGGAGCTGAGCCTGAGTGGACTCTGCCAGTGCCTGGT
GGAACTGTCCACCCAGCCGGCCACTGTGTGTCATGGCTCTGCAACCACCAGGGAGGCAGCCCGTGGTGAG
GCTGCCCGCCGTGCCCTGCAGTACCTCAAGATCATGGCAGGCAGCAAGTTG

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향 (1)

# data[].attributes.attachment_link.readable_text
The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries. The purpose of this study is to; 1) quantitatively evaluate the memory-enhancing effect of YMT_02 by behavior task, 2) identify candidate genes responsible for enhancing memory by cDNA microarray and 3) assess the anti-oxidant effect of YMT_02 on PC12 cell. Memory retention abilities are addressed by passive avoidance task with Sprague-Dawley (SD) male rat. Before the training session, the rats are subdivided into four groups and administrated with YMT_02, Ginkgo biloba, Soya lecithin and normal saline for 10 days. The retention test was performed. 24 hours after the training session. The retention time of the YMT_02 group was significantly (p<0.05) delayed (～100%), whereas Ginkgo biloba and Soya lecithin treatment delayed 20% and 10% respectively. The hippocampi of YMT_02 and control group were dissected and mANA was further purified. After synthesizing cDNA using oligo-dT primer, the cDNA were applied to Incyte rat GEMTM 2 cDNA microarray. The microarray results show that prealbumin(transthyretin), phosphotidylethanolamine N-methyltransferase, and PEP-19 are expressed abundantly in the YMT_02 treated group. Especially, PEP-19 is a neuron-specific protein, which inhibits apoptotic processes in neuronal cell. On the other hand, transcripts of RAB15, glutamate receptor subunit 2 and CDK108 are abundant in control group. Besides, neuronal genes involved in neuronal death or neurodegeneration such as neuronal-pentraxin and spectrin are abundantly expressed in control group. Additionally, the YMT_02 shows an anti oxidative effect in the PC12 cell. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the memory-enhancing effect of herbal extracts YMT_02, for example, anti-apoptotic, anti-oxidative, and neuroprotective effects.

# data[].attributes.attachment_link.description
The herbal extract (YMT_02) is a modified herbal extracts from Yukmijihwang-tang (YMJ) to promote memory-enhancing. The YMJ extracts has been widely used as an anti-aging herbal medicine for hundred years in Asian countries.

# data[].attributes.body
Homo sapiens 5-hydroxytryptamine (HTR3B) gene, complete cds

EF444985

FASTA

CAGAAGGTTTGACTTAGAGTCATAGCTTACTACAATGCATGGCAAGAATGCAATGTCGATCATCGCCTCA
ATTAAACCTTTGCATAAAGGGATGCTTCTGCATTGCTTTCCCATAAAAACTGCTACCTACATAGCTATAT
TGCCTGTAGCTTGCCTTTCTCTTGTCCTAATTCATCCCATAAGTAGAAGCTGACAATTCAAAGAACTGAT
AGGAAGAGCTGAGACTTTGAGGGCCAGATCAGTCCTCTATGATCTTCCCTCTTGATGAAGCCCCATTTAT
CTGCCCACCCTCTCTAAAGAGTATTTCTCAGATAAACTTTCCATAACTGTGTACCAAAGTTCCTCCACCA
AGTTACATTTAAATTACTCTTTCTTCTGAAATATTTTCAAATGTATAGCAGAATTGGATGGATAGTACAA
AGAATTCCCCTAGACCCCCACAATCATTTGAGAATAAGTTAGCGACATGATCAACATGATGTGCCCTGTT
TTTGTTTTTTGTTTTTTTTTTCTTTTCTGAGACAGAGTCTCATTCTGTCGCCCAGGCTGAAGTGCAGTGG
TGTGATCTCGGCTCAGTGCAACCTCTGCCTTCTGGGTTCACAAGATTCTCATGCCTCAGCCTCCTGAGTA
GCTGGAATTACAGGCATGTGCCACCGTGCCGGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACC
ATGTTGGCCAGGCTGGCCTTGAACTCCTGATCTCAGGTGATCTACCCACCTAGGTCTCCTAAAGTGCTGG
GATTACAGGCGTGAGCCACTGTGTGCGATCTTCCCTCATTCTTGAATACTTAGTGTGCTGGTCTTACAAA
CAAGGACATTCTTTTGCATAATCTCTATACAATTAAAATCAGGAAACCAACATTACTACATTGCCACATT
ACTACCATCTAATCCACATACCTCATTCGAGTTTCAATTCTTCCCAAAATGTTCTTTATAACAAAAGGAT
CCAATTCAGAATCAGATATTGTGCTTGTGTCTCTTTAGTCTGATAGGTTCCTCAGTCTTTCCTTAACTTT
TATAACCTTGGCACTTTTGTGTGTGTGTGTTTTTGTTTTTGAGACAGGGTCTACCTCTATTGCCCAGGCT
GGAGTGCAGTGGCACAATCACAGCTCACTGCAGACTCTATCTCCTGAGCTCAAGTGATCCTCCTGCCTCA
GCCTCCCAAGTAGCTGGGACTACAGGAGTGCACCACCACATTAAACTAATCTTTTATTTTTTTGTGTGGA
GACAGGGTATCCTTATGTAGCTTAGGCTGGTCTCAAATTCCTGGGCTCAAGCAGTCCTCTTACTTCAACC
TCCCAAAGTACTGGGATTACAGGTATGAGCCATTGCACCTGGCCCACCTTGGCACTTTTGAAGATGCCTG
CTGTTTTGTTTGTCCCACAGTATGCCACTGTCTCATCTGGTGTTTCCTCCTGAATAGATTCAAGTTATGA
ATTTTTGGCAAGACTATACCGGAAGAGCTATGGAAGCTAGGTGTGCTTGTTACTATTGGGGTGTCATTCT
CCTAGGCTGCTAAGTGGACAGAGCTAGGGAATATATGCATGTATGAACATGTGCAAACACACATTACATT
TGTATTTATTTCTTTTCTTTTCTTTTTTTTTTTTTTTTTTTGAGGCGGAGTCTCCCTCTGTCTCCCAGGC
TGGAGTGCAGTGGCGCGATCTTGGTTCATGGCAACCTCCACCTCCTGATTCAAACAATTCCCCTGCCTCA
GCCTCCCGAGTAGCTGGGATTACAGGCATGTGCCACCACGCCTGACTAATTTTTGTAGTTTCAGTAGAGA
TGGGGCTCACCATGTTGGTCAGGCTGGTCTCAAACTCCTAACCTCAAGTGATCCACTGGCCTCGGCCTCC
CAAAGTGCTGGGATTACAGGCTTGAGCCACCACGCCCAGCTTATTTGCAATTATTTATATATTTAGCAAA
TGTACTGAAAACCATGGGTTCACACTGATACCTCCAAAACCAAGTCAACACCAAATGGTTATCTTAGTTT
TCTCTCTTTCTGTGTATACCACTCCCTTCACCAACAGTAAGAAAGCAATTAGGCTACATTTTATTGGTAG
ATGAAAATTAGGTGTAGAACTTCATGGATTCTAAACAAAGGGACTGAGTGATTAGGAGAATAGGAATCAA
ATATATACTTTCCCCCCTTCGGTTGGCTCTGTGAAAATTTATATCAAAATTTGCCTCTATAATCTCATTT
TTAATAAAAAAGCAGGCAGATGTTGGAAATGGTATTTGTTTTTTCTTTCTAGAGCATCTGGGGGTGTCTA
GAAATGGAAAATAGTGTTATTGTATAGAATTATGCATCATGTTCCTTAGTAATGAAAAATGAATGCGTAT
TCAATCAAAAAGTGAAAAAAAAACTTTTATTAAATGATGTTGAACTTTGTTTTCATCTTCAATAAGCCTA
GAAGCATCCTTTCTAGAAGGTTACTTTCTGAATTGTGAGCAGTGAAAATTTGAGAAGCCCTCCTATTTAT
CAAAAGTCAATGTTTCTTTTAATTAAAAGGGCTTAGGAAAAACAAAAAGCACTTTTCCATTGCATTGTTT
GCAGTTGTTCTTCATTAGACAATACCAGGTTTCCATTAGCCTTGTGTAATTCAAGAGCCCAAGAACCACT
GTCAAATGCTGAAGATGTTACTGCATAAGTCTGATTTAGAACAGCTATTTGGATTTCAATTATTAATGAA
ATTAGTGTCCTGAATGTCAGCAAGAGTACTGCCTTAGGTAAAGGCTGTAAAGGAAATCATATTAGTGTGG
AATTTCCTCTCCTTAGGGGCTCCTCTTAGATTACATTATTCACTTAGCTATCTCCTGTCAGGAAAATGGA
AATGTTGGCCACGGTCTGATCTTGGGGCAGAACCAAAGGGAGAAGTTAAGGGATTTCATGACGGCATCAA
TTCCAAAACATTTGCATGGTGCTGGTATTGCCTTTGGTCCCACTGAGTGTTTACAAAATAGCACCAGTAA
GGATAGCATCAACTGGCAAACGGAGAAGGAGGAGAACAGAGTGGAGAGGAACCCTGTTAGGAGAAATTGA
GCGGCATTCCATCTGGTAGGCAAGTTTGCATTTCTCCTTTTTGGGATCTGCCCAGGAATGTTGTCAAGTG
TAATGGCTCCCCTGTGGGCCTGCATCCTGGTGGCTGCAGGTGAGTCCTTTTAATAATTTTACTAGGCATT
AAGGATTAACTCTTAGAAGATAGAGACAATATTTGTATACACCGTACTGTAGGACTTCTCAGGCATGTTT
GTTTTTATTCATCGTCTGTAAAATGCCAGTCCTGTGAAGCTATATGGAAAACAATGATTTGTTTGTGACT
TGGCTCCTATGATTGCAAATACGTATACATATAGCATCAGACTGTGGGGCTTTTTACACATAGCTGAAAA
TATGCTCTGAAATGTTATAATTGCTTGATAAATCTCTGCTTCTCTAGAAATCAAGCTCTCTGCAGGTTGA
TTGAGAGTTTGGGGCATTCTTTTTCAAATTTGCTTTTATGTGTGGATAATATGTTTTTATTATCCAAAAT
AGTCTTGGCTTCATTATCAGCTGTTAGCAGCCCAGAGAAAAAGTCCACTAGATTTGTCATGGCTACAGAT
ATATGTATATCATTTTGGTATTAGAGGCCTTAACATCTCGCCTTACAAAATCTATATAGGTGATGAGTAT
ATCTCTAAAGACAGGTACTATAAACTGGTAAGTTTTGAGTCTACAATTCCTCTTAAAAGTAATTTAGAAG
TTAATACTTTTTTTTCTAAATTTCTAGAACTATATTTCCATGGCACCAGTATTAACTCTACTGAATTGAA
TCATTTTAGTTAGGATACAATTGATTCTTAGTACATGCGCACAAATCAAGAATTTATGATTATGCTGGTG
TTCTCAACACCCCACTTTCTTGAGGTTTCTCCTAGAGCTCATATCTGAATTCTCAAGTTTTAGGATGTAA
CTGGAAGTGGAATGAGTCAATTATAGAGATGAGTTAGAAATTTGGTAGCCAGGAAGCTGGAAATGATTTT
TTTATTGGGTAGAGAAGAAAAATAGCCACTTATCATTAAAGTATAAAAATCCTGGATGTTGAACATAAAG
GATAAAAGCATATCTTAATTGTAAAAAATAAAAACAGAGGAAGTGTCATTAAATGCCAAATGGGTTTCTT
AGTAGAAAATAATCGTGAACATTAAACTAAGATAAAATAGAAAAATGAAGGTAAGAAAACAGACTAAGTT
CTTAACAATATCTCTCTAATGTTTTGTATTATGCATGATTTTTGTTCTAAATGTAGCAGAAAGAAGTGTT
TTAATAGTGTCTGATAACCAGTGCTTTACCATAAATCATTCCGTGGATACTTGTTGCAGAAAGACCTGCC
CTGTGGAATTGTACTGGGTTTTACTATGTTTACTTTACGTATTTTACTATATTTACAAATCAAGATGATT
TGTTAAGTGTAAGAGCACGGAACCAATCCCAGGGGGCAGTGTGCTGGTATAGAAGGAGGCCTGAACAAGG
AGAGCCAGCTAATTCCTGCTTCACCACTCACCAGCCACGTAACCCTGGGAAGTCAATTAATCTCTTAGAG
ACTCAGTTTTCCTGTAAGCTAACTTTATTTTGGATATTCATGACAATCAGATAAGACAGCATTGTTAAAT
TGTAAGGTGAAATGCAAATGTAAGATATTATTAACCTGAAAACACATGCAGAAAATACCACTGGCTATTT
CTGTCCAATAATATACTCCATACAACATGTCAGTCTTCTTTTTGAAAGGTTACTTGCTGTTGTTCCTGAT
ATTCTCAGGGAGCCAGGTTACACTTAATCATGTAGCGTCATGATTAAGAGCTTGGACTTCGAATCCAGAC
CAGCTGTGTGTAAATATCACTCCCTTCACTTGCAAGCTTCATGACCTTGGGCAGTGCTAAGCTCACTATG
CCAATAGTCCCTCATCTGCAGGAATAGTGCACTCCTCACAGAGCTGTAGAGTACCTGTCTCATAAGTTTG
TTGGGAACTTTGAAGGAGATCATTCATGTGAGGACTTAGCCTGGTGCCTGGCATACAGTCAGCATTCAAT
TAGTGTTAGGAATTCAATACATAAATCTCATTTCCTTTTAACCTCTCAGATATATGTGTAACTCTCTTAT
AATCCTACAGATACATAGTGACTAAAGCAAGAATAGTGCTTTCCATCAAAGTGCCCTGAACACTTTAAGA
TAGGTGCATGGGAGAGTAAGTCATTAAAACAAGGAAAACACAGGAATAATTTTACTGTGTTCTTATCTTC
CTCCTTTTCAATCTCTCTTCACCTGCAAGTGCTTCATAGCTCAGTCCCTAACACATTCTACTTTCCAGAA
ATCTTACAAGTGAATCATTTGAAGCTGATTATTAGCATTCAAGAAGGCAGTTTTGGCGAGGAATCTTTTC
TGGGTCTGTTGATAGCTTCCATCAGTGTCTGTTGAAACATCACTGAACTGTGAGTCATTTAGTGCTTGTG
TGAATGTCACATTAGCTTTCAAGGGGCATAATTTACTGTTAATTAATGGTCACTTAAATCCTTAATCTCT
CTCCTGTAATTGATCTTTCTCCTCAGGCAAATTTGCATTCATAGTAGAAATGTAATTTATTATTTCTTCC
CATCTTAAACAAAGAACTCTTTCCCCTTTTGGTTTATTTAAGCATATTTCCATTTGCTTATTATTTTTCT
CCTTTCAGTGCTTGATTCTCTTTTGTTCCTAGTCCTAGCTTCCAGAACTTTCTCTTTTTAACCAAGATTT
TGACCCATTCTAGAAAACATGAGTTTATCCATAGGGAATCTATGGATTGCTTCCTAGACAAGATCGCAGA
GGGGAATTTGAGTCTTTGTGAGCAGACAGTGCGAGGAACACATATTCATTTCTCAGAATTATGTTTTAAT
GCTGTCTCGGGTAATTTCCTGTATTGAAACATACTCATTTCGGTGTTTCCCCAACTGAGACTTGAATTGG
GAGCTTCCAAGCGAGGTAAACGTGAAGCTGCCCACAGCAAAGTTGGCAGGAAAAGCACTGGGACAGCTTT
GCCTTGTGCTCCTGACTTCCCTCCCCTGGGGTACACCAGCGAGAAATGTTCTCAGGTAGAGAGATTCACA
GCACACTTTTTGACAATAACAGTTCACAAAAACCCTACAAATGATGCCTGATGCAAGCCACACACCTTAG
TCCTGCACGCCTGAATAGCTAACGCCATTTTAGGCATCTTCTTGGGTGGATTGTTAGGGAGTTAAAATGC
TCATTAGATTCCCAGTTCCGACGGTAATGAAAGTGTATATTGCACTGCCCACAAGGAGTCTGGCATTGTA
CCTCCAGCATTGTTGTTAGCGTTTTGGTCACACTGACATTGTTAAATCATAGAAATTGCTGCCTGTAGCG
GCAAGTGACATGACATCATTCATAAAGAAATGGGTATAAACAGGATTGTCGCACAGGGAGAAGCGATCTC
ATTTACAGTTTCCTGCTGGATCCCTCATACCTTTGGCAGCTCATCAAAATAACCATCAAAACCACTGACA
GCCCAGGCAAATTCACACGCAGGACACTACAGAGAGATTTCACTCACGCACTTTGTCAGGAGGTGTGCAA
TTTGCTTCGGTCCAATGACTGAGTGAAGCATTGAATCCCCTAAGGGCTGGCAAAGCTTGTGAACAAAATA
TCTTTTTGCAATTCAGAGAAAGCCCTCACAAATTTGTCACTTCCAATTGCTGGTGCTCATTTCTCTTGCT
TCCCTTCCTCTGGCCTCCCTGGCCTCAACACCAGTCACAGCTCCTCTACAAATCAAGATTTTTGGGTCAG
AAGAAATGGTTGTTCCCTGGAACCAGAGAGAATTTTCTCAAAACAAAGATCTCATTTTCTGACAAGTGCA
TTTTATTTAAGGACAGATGAAATGAGAAAAGAGATTTTTGAGGAGAAAAGTACAAATTGGGCTCTCGTTA
ATCCCTTTATCTAGAAATCTATACAAGTTTTTTGGGTTAATGGGTTTCTTCATGTTTTTTTGCTTGATTT
TTCTATTGAATTTTTGAATTACGCATGAAAAATCAAAAGAAAAACCAACGCGGGAGTTAAGTTTTGGCTA
AGTGTGTAGGTGGGTTTTTTCTAGATGCTAGGAGAGGATGATACAGGAATAAAGAAATTATTCTGTCTCC
AAAATGCAAGATTGTTGTAAAATGGAATTGTAGGTACAGTTTTCCAACCTTGGAGATGGCGTTGATTTAC
TTGTTTTTACAATGAGGTTTCTCCATTGGGCACTTAGGCCATCAGCTTACTTTAAGCATTTGCCAAAGGC
CCCACCAGCTGGCTGTGAATTGATGCAGATTTTGGTGAGCTGAAAGCTATATTCTAGTAAAAGCCACCGA
GTCCTGAACAGTCTGAAACCTCCTAAAGAAACCAAGCTCCTCTTACTTTTATCTTCACAATGATAGTTTA
TTTTCCAGGAATTCTAGCCACAGATACACATCATCCCCAGGATTCTGCTCTGTATCATCTCAGCAAGCAG
CTATTACAGAAATATCATAAAGAAGTGAGACCTGTTTACAACTGGACCAAGGCCACCACAGTCTACCTGG
ACCTGTTCGTCCATGCTATATTGGATGTGGTAAGGACCATCTTGCCCCTTCCTATTCTTGTTAACGTCTT
TTGAAACAGTCTGCAGTTCATGCAGGAGCCTATGAGAGGTTATATTCTTGAGATTGTAGTTGAACTGGCA
GGGAAGTTGCGCTAGATTCATTTTAGCAGTTAGGTAAAATATTCATTAGGCAAATCATGCTTTCTAGCCC
CCAGTGGACACTGTGAAGGGCAAATTGTCTAGATCATGGAATTAAGATGTTTTAAGAATTGTGAAGCTGG
GTGTGGTGGCTCATGCCTGTAATCCTAGCACATTGGGAGGCCAAGGTAGGCAGATCACTTGAGCACACGA
GTTGGAGGCCAGCCTAGGCAGCATGGTGAAACCCTGTCTGTACCAAAAATACAAAAACTAGCCAGGCCAT
GGTGATGCATGCCTGTAGTTCCAGCTACTCAAGAGGATGAAGAGGGAGAATAGCTTGAGCTTGGGTGGTT
GAGGCTGCAATGAGCCATGATCATGCCACTGCACTCCAGCCTGGGTAGCAGATCCAGACCTTGTCTCCAA
AAAAAAAAAAAAAAAAAAAAAAAACTTGTGGTCATGGATCACAGTACATATTGTATGAATATTGCTGAAA
CCAAAAACAGTAATAGGTATTTTATCCTATGGTTCACATTTATTATGATAATAACAGATTTAAGAAGAGA
TTCACTACTTATTGCTTGAGATTTAAAAGAGAGAAAAAAATGCCTTATTATCTTGAGTTTATAAGGGGGC
TTTTATACCTAAAGCGTTTGCTAAGGATGGTGGGGAGAATTTGTTTATTAAAGCATCCTTTTTCTCCTAT
GTCTGAAAGATGGGCTGTGGGAATGAAACGAACACTGTGAAGTAGTTGGCAGAGTTCTTAGGAAGCCATC
AACAAGTGATGATCCTGATGGTTAGAACCTCTTCCTCTTCCTCCTTTTTTCTAATTACAAAGTCTTTGAT
TTCAATTGGTCCCAGAGTTATTTTGATTTCTCTCTCTTTTTTTTTTTTTTTTGAGACGGAGTCTCTCTCT
GTCACCCAGGCTGGAGTCCAATGGCACGATCTCGGCTCACTGCAACCTTTGCGTCCCCGGTTCAAGCAAT
TCTCCTGCCTTAGCCTACCTAGTAGCTGGGATTACAGGCATGCGCCACCACGCCCGGCTAATTTTTGTAT
TTTTAGTAGAGACGGGGTTTCACCTGTTGGACAGGTTGGTCTCAAACTCCTGACCTCAAGTGATCCATCT
GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACACCCGGCAATTTCTCTTAAAAACAG
ACTTTACTGAGCTCGTTTTGTTAAAGTAATTTTAATCATTGTTTTCCCATTTACTTCTCAAAAATTGTTT
TCTTTTCTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTCGCCCAGGCTGAAGTGCAGTGGCACAATC
TCTGCTCACTGCAAGCTCCACCTTCTGGGTTCATGCCATTCTGCCTCAGCCTCCCGAGTAGCTGGGACTA
CAGGCGCCCGCCACCATGCCCGGCTAATTTTTTGTATTTTTAGTAGAGATGGGGTTTCACCGTGTTAGCC
AGGATGGTCTCGATCTCCTGACCTCGTGATCCATCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCG
TGAGCCACTGCACCCGGCCAAAAATTGTTTTCTAATTGAGGTAAAATATACATATGTGGAAATACACAGA
TATTAAGTGTACAAGCTGATGAATGTTGCTAATTACATATACCCATATAACATTTATTTATTTATTTATT
TTGAGACAGAGTCTGGCTCTGTCACCCAGGCTGGATTGCAGTGGCACGATCTCAGCTCACTGCAACCTCT
GCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGCGCCACCA
CATCTGACTAATTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGTCAGGCTAGTCTCAAACTCC
TGACCTCAAAAGATCTGCCCACCTCAGCCTCCCAAAGTGTTGGGATTATAGGTGTGAGCCACCACACCCG
GCTATTTATTTATTTTTTGAGATGGAGTCTCACTCTGTTGCCCAGGCTGGAGTGCTGTGGCACCATCTTG
GCTCACTGCAACCTCCGCCTCCCGGGGACAAGCAATTTTCCTGCCTCAGCCTCCTGAGTAGCTAGGATTA
CAGGTGTCTGCCACCACACTCGGCTTATTTTTATATTTTTTAGTAGAGATGGGGTTCCACCATGTTGGTC
AGGCTGGTGTTGAACTCCCGACCTGAAGTGATTTGGCCGCCTCAGCCTCCCAAAAGTGCTGGGATTACAG
GCATGAGCCACGGTGCCCGGCCATATAACCCATATCTCTATCAAGATATCAAAGATCCCCGAAAAGGTCC
CTTGTTCCCTTCCTAGTCCATCCCCATCCTCTTCACCCACAGAGACAAATACTCTTCTGATTATTTCTTT
GAGCAGTGATTAGTTTTACTGGTTGTAGAACTCCATGTGATGAAGTTTTATTGTGTGTACTCGATTGTGT
CTGGCTTTTTCACTCGGCATTTTGAGATTCACTTATGTTGCGGTGTGTATCAGCAGTTTGTTCCTTTTTC
TTAATAAGTAGTATTCAGTTATGGCTATATCATGATTTGTTTATCCATTCTCCTGTTGACAGGCATTTGG
GTTGTTTCCAATTTTAGGATAGTATGAATAAAGCTGCTAAGAATTTTCCTGTACGAGTCTTTTTGTGTAT
ATGTTTCCATTTCTTTTTTCTTTTCTTTTTTCTTTTTTTTGAGACCTTGCTCTGTTACCCAGGCCGGAGT
GCAGTGGCACGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTC
CGGAACAGCTGGGACTACAGGCGTGTGCCACGACGCCCAGCTAATTTTTTGTATTTTTAGTAGAGACAGG
GTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCCGCCTCGGCCTCCCAAAG
TGCTGGGATTACAGGCGTGAGCCACCGTGCCCGGCGTTTCCATTTCTTTTAAGTAAAACCTAGAAGTTCT
GTGACTGGGTCACAGGAGGGTGTATACTTAATGAGAAACTGCCATACTGATTTGCAAAGTGGTTGTGCAT
TTTACACTCCCACCAGTAAGCTATGAGAGTTCCTATTGCTACACATCCCTGCCAACATTTGGTGTTACCA
GTCTTTTTAATTTTAGTCATTCTAGTAAGGGTATAATGGTATTTCATTGAGGTTTTATTAAAATTTCCCT
AATGGCTAATGATTTTGAACTCTTTTTCATGTGCTTATTGGCTACTTATAAATTTTTCTTTGTGAAGTGC
CCATTTAAGCCTTCTCTTATTTTTAAAATTGGGTCATTGGTTTTTTTTTATTGTTATTATAGATTTATAG
GAGCTATTTATACATTCTGGGCACAGTCTTTTTTTAGATATTACCTCCTATATTATATAATATGTATTTA
AATCATTATTATAATATAATAACATATAATATAGTAATTACATAATACATACACATATTATATAATAAAT
GTTTATTCCCAGTCTGTGTCTTCGCTATTCATTTTCTTAATGGGTCTTACAATGAATAGAAATTTTCAAA
TTTGCTGACATTTGTTAGTTTGTTATGGTTTGAATGTGTCCCCAAAATTTCTTTCTTTCTTTCTTTGTTT
TTTTTGAGACAAAGTCTCAAAGGCTGGAGTGCAATGGCACGATCTCGGCTCACTGCAGCCGCCGCCTCCA
GGGTTCAAGTGATTCTCTTGCCTCAGCCTCCCCAGTGACTGGGATTACAGGTGCCTGCCACCATGTCTGG
CTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTATTTTTAGTAGAGATGGAGTTTCACCATGTTG
GCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTAC
GGGTGTGAGCCACCATGCTTGGCCTTTTTAAAAAAAATTTTATTTTTAGATGAAATCTCTCTCTGTCACC
CAGGCTGGAGTGCAATGGCGCAATCTCGGCTCACTGTAACCTCTGCCTCCCAGGTTCAAGCCATACTCCA
GCCTCAGTCTCCCGAGTAGCTGGGACTACAGGCACGTGCCATCACACCCGGCTAATTTTTCTATTTTTAG
CAGAGACAGGGTTTTGCCATGTTGGCTAGGCTTGTCTCAAACTCCTGACCTCAGGTGATCCACCTGCCTC
GGTCTGCCAAAGTGCTGGATTACAGGAGTGAGCCACCACGCCCGGCCTCCAAAATTTCATCTGCAAATTC
ATATGTTGATGGTATTTGGAGGTGGGGCCCTTGGAAGGTAATTAGGATGGTGGCTATATAAGAAGAGGGA
GAGAAAGCTGACCTGACATGCTCTTGACCTCTTGCTGTGAGATGCCCTCCTCCATGCTACGATGCAGTGA
GGAAGCCCTGATCGGATGCAGCTCCTTGAGCTTGAACTTTCCAGACCCCAGAGCTGTGAAAAATATATTT
TTTTTTTTTTTATAAATTACACAGTCTCAGGTTTTCAGATACAGGAACAAAAAACGGACTAAGACAAAGT
CCATTTCAATGAACAGTTTTTTTAATTTTTAAAAAGATTTAAAAAATGTTTTAAAAATTAAATTAAATTG
GCTGGGCGCAGTGGCTTACGCCTGTAATCCCAGCACTTCGGGAGGCCGAGGCAGGTGGATCACCTGAGGT
CAGGAGTTTGAGACCAGCCTCAACGTGGAGAAACCCCGTCTCTACTAAAAATACAAAATCAGCCAGGTGT
GGTGGTACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCG
GAGGTTGCAGTGAGCCGAGATCACGCCATTGCACTCCAGCCTAGGCAACAAGAGCGATACTCCAACTCAA
AAAAAAAAAATTAAATTAAATTAATTTTGTTTTTTTGAGACAGTCTTGCTCTGTTGCCCAGGCTGGAGTC
CAGGGACACAATCTCACCTCACTGCAACCTCCGCCTCCTAGCTTCAAGCAATTCTCGTGCCTCAGCCTCC
CAAGTAGCTGAGATTACAGGTGTGTACCACCACACCTAGCTAATTTTTGTATTGAATTTTATTTTCTAAT
TAATATATATTACACGTTTGTTGATGACAGGCATTAGACTCTAGTCTTCTCATCTGGTTTTGGTATCAGG
ATTGTGTTGGCCTTATAAAACAGAATTGTTGAGAATTGTTTCCTTCTCAGTTTTCTGAAGGCGTTGTATA
ATGTGAATATTTTTTCTTCCTTACTGTCTGATAGAATTTACCAGTAAAACTATAGGGGTCTGGAGCTTTC
TTTGTGGGAAGGTAATCAAAGGCAAATTCAACTTTATTTACACATATTCAGTTTTTTCTTGTGTCAGTTT
TGCTAATTTGTATTTTTAAGAAATTTGTCTCTTTCATGCAGTTTGTCAAATTTATTGACTTAGTTGTTCA
TATATTTTTTTCTATTATTTTCATGCCCATAAGATCTCTAGTGATATGGCTTTTGTGTCTCATATTGGTA
ATTTGTGTTTTCTCATATTTTCCCCTGACCAATCTTGTCATAGTTTTACCAATTTTATTTTTTTCAAAGA
GCCAACTTTGGGCTTTGTAATTTTTTTGTTTGTTTGTTTTGTTTTGTTTTGCTTTGAGACAGGGTCTCAC
TCTGTTACTTAGGCTGGAGTGCAGTGGCGTGATCACGGCTTACTGCAGCCTCAACCTCCCATGCTCTTTA
GATATCTTTACATATTTCTTTTCTTCTACTTACTTTGTTTGTGTTTTTGAGATAGAGGCAAGAGACAGAC
AGAGTCTCTGGGACCCATAATCTGATTTTTTTTCCCTCTAGTTTTGGCTTATACACAATATTATACAAAT
GGAATTATATGTTATTTTACCTTTTGTGCATGGCTTCTTTCACTTAGCATAATGCCTTTGAGATTTATCC
ATATTGTTGCATAACTCAGTAGTAGTATTTTATTGTACAGATGCACTACAATTTATCTGTTTACCAGTTG
TGGGACACATGGATTGTTTCCATTTGGGGGCAATTATGAATAAAGCCACTATAAACATTTGCATATGTTT
TTCTTTCTCTTGGATAAATGCTTAGGTATAGTATTGGTGAGTTGTATGATGGGTCTATGTTTAATTTTAT
AAGAAACTGTCCAGCTTTTTTCCAAAGTGGCTGTTATCATTTTGCATGTCACTCATAATGTGTGAGAGCT
TCAGTTTCTCCACATAATCACTACTCCTTGAAGCTGTCAATCTTTAAATGTTTTTTTTTTAGACGGAGTC
TTGATCTTGTCGCCTGGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGCAACCTCTGCCTCCTGGGTT
CAAGTGGTTCCCCTGCCTCAGCCTCCTGAGTGGCTGGGATTACAGGCACCCACGACCGTGCCTGGCTAAT
TTTTGTACTTTTAGTAGAGATGGTTTCGCCGTGTTGGCCAGGCTGGTCTCTAACTCCTGATCTCAGGTAA
TCTACCTGCCTCGGGCTTCCGAAGTAGTGGGATTATAGGCATGAGCCACCGTGCCCGGCCCAATCTTTAA
AATTTTAACCATTGTAGTAGGTTTGCTGTAGTATCTCAATGTAGTTTTTAATTTTTATTTCCCTAACATT
TGAACATGTTGAACATTTTTTCATGTGCTTATTTGCCAATTGCCAACTGTATATCTTCTTTGGTAATATG
TCTGCTCAAATCTTTTATTTAATTTTAAAGTTGGGCTGTTTATTATTTTATTATCAGGTTACAAGAGTTA
TTTATATACTCTGAGCTTATTTCTTGACTCTGTTCTATTCAACTAATCTGTATGTTTGTCTTTCTAACAA
TACCACACTGTTTTCATTACTGTAGCATTATGGAAGTCTTGAAATAAGATAATGTGAATCTTCTAATCTC
GTTCTTCTTTTTAAAAATTGTTTTAACTTTTCTAGGTTGTTTGCATTTCCATATAAACTTTAGAATTGAC
TCATCAATGTCTATTTTTAAAAACTTCTGGAATTTTGATTGGTACTGCACTGAATCTATAGATAAATTTG
AGGAGTACTGACATCTTAACAATACTGAGTTATCCAGTCCATACATACAGTGTATCTCTCTGTTTAGTTC
TTTAATTTCTTTTATTAATGTTTCATCATTTTTAGCGTTTTGCATACATTTGGTTAAATTTATTCCTAAG
TATTTCACATTTTTTGTTATTATAAATGGTATTTTAAATTTGAATCTAGTTGTCTGTTGCTAGCATACAA
AAACACAGTTGATTTCTAGATATTGGTCTGGGATTCTGTGACCCTTACATTTCTTTTTCGCTTAAGAGGC
TTTTTTTGGTAGATTCTTTCAGATTTTCTGTGTAGAAAATCATGTTGTCTGTGAGGAAAATCAGTTTTAC
TTCTTTCTTTCTGATCTTTATACATTTGTTTCCTTCTAGTGTCTTGTTACCCTGACTAGGGCCTCCAGTA
CAATGTTGAACAGAATGGTATACTGCTCTATAAATATTAATTCAATAAATGTGGTTGATAGTGGTGTTGA
AATCTTCTATTTTTTTTTTTTTTGAGATGAAGTCTCACTCTTGTCCCCCAGGCTGGAGTGCAGTGGCGCA
ATCTCAGCTCGCTGTAAACTCCGCTTCCCGGGTTCAAGTGATTCTCCTGCGTCAGCCTGCTGAGTAGCTG
GGATTACAGGTGCCTGCCACCACGCCCAGCTAATTTTTCTACTTTTATTAGAGACGGGGTTTCACCATGT
TGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGGTGAGATT
ACAGCCGTGAGCCACTGCACCCGGCCGAAATCTTCTATTTTTTTTCTTCCAGTTGTTTTATTAACTTGAT
GGAGTGGAATGTTAAAATCTCCAGTTATGATTGTTGTTTGTCTATTTCTCTCTTTGGTTCTGCCAACTTT
TGCTTCATGTATTTTGAAGCTATGTTATTAGGTGCATACACATTTAGACTTATATTTTTCTTTGTCTTTG
GGGGTTTTTTTTGAGACAGTATTTTACTCTGTCTCCCAGGCTGGAGTGCAGCGGCATGTCTCACTGCAGC
CTCAACCTCCTGGGCTCAGACAATTCTCCTGCCTCACCCTCCTGAGTAGCTGGGACTACAGGCACATGCC
ACCACACCCAGCTGGTTTTTGTATTTTTGGTAGAGACGGGGTTTTGCCATATTGCCCACCATGGTCACTA
ACTCCTGAGCTTAAGCAATCTGCCCACCTTGGCCACCCAAAGTGCTGGTATTACAGGTGAGCCACTGTGC
CCAGCCTATATGTCTTCGTAATGAACAGATCCTTTTACTGTTATGAAACGCTCCACTTCATCTCTGATAA
TATTCCCTTTTATGAAGTTTTTCTTATGCTTGCTTCCCACATGGCATATTCTTGGCCATCCTTTTATTTC
CAACCTATTTGTATCATTTGATGTCCTTTGTATTTGAAGTCATTTCTTATAACAATATATAGTTAGGTAC
ATGCTGAAAAGAAATATATAGGCAGGCCCTGTGTACACTTCACTCAATCTCTCATACTGTTAACATCTTG
CAAATATATATATATTACAGTGTCAATACTAGAAAATGAACACTGGTACAATCTATAGAGCTTATTCAGA
TTTCTCCTGCTTGTGTGTACTCGTGTGTGTGTGTGTGTGTGTGTGTGTGTAGTTCTATGTAATTTTATTA
CATGTGTAGCTTTTTGTAATCAGCACCACAATCAAAGTACATAATTGTATCAACACCACAGGGCCCCCAG
GTGCTATCCCTTTATAGCCACATTCCTTTCTCTCTGCTGACTCCTAACCCCTGGCAACCACTAATCTTTT
CTCCATCTTTAGGCTATTTCAAGAATATTATATAAATGGAATCATACAATATACTACCTTTGAGATTGGC
TTTTTTCCTTTAGCATAATTCCCTTATGGTCCATCTGTGTTGTTGTGAACGCCAATAGTTCATTTCTTTT
TGTTGCTGAATACTATTCCAGGTTAGGGCTTTTTTTTTTTTTTAAACCAGTATGACAATCTATGCATTTT
TTTTTTTCAGTATGGAGTCTCACTCTGTCCCCCAGGCTGGAGTGCAGTGGCCCAGTCTTGGCTCGCTGCA
ACCTCCACGTTCTGGGTTTAAGCAATTCTCCTGCCTCAGCCTCCTGAATAGCTGGGATTACAGGCATGCG
CCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGACCAGTCTGGTCTT
AAACTCTTGACCTCAAGTGATCTGCCTGTCTTGGCCTCCGAAAGTGTTGGAATTACAGGTATGAGCCACA
GTGACCAGCCAATCTATGCATTTTAGTGGAAATGTTTAGCTCATTTGCCAGTACCATCTTGCTATTTGTT
TTCTAGTTTTCTCATCTGTTTTTTGTTCTATTCCTCATATCCTACTTTATTTTCCGGAATTTTTAAATTT
TTAATAAATGTTAAAAATATTTTAAATATTGGATTATAGTTCCTCTATTAGCTTTTTAGCGATCCATCTT
TGTATTTTTTTTAGACTTTGCCTTAGGAATTTCCATATTCATCATTAACTTATTACAGTGTACTTAGAAT
TAATATTTTGACACTTCACATAAAATGTACGAAACTTACAACAGTAAAATTCCATTTACCTGCTCCCGCC
TCTTTTGTGCTACTTTTATCTTCAATTTTACATTTACATACAAAATATACCCACAAAATGATGTTATTAT
TTTAAGGTAACCTGTTAAAATAATTTAATGATTCAGTTTCAATTTATTTTAATAGGGATGTTTTTCTATG
TTACTATTTTAGTACTACCAGTGAATCAGAGTCTTTGATTTTTTGGGACTGAGAATTAGGCATTTAAATC
AATGAAAAAATTAATTTGAAACTTAAGAGTACAAGGTTTAGGCAGGGCGCTGTGGCTCACGCCTGTAATC
CCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTCAACATGGA
GAAACCCCTGTCTCTACTAAAAAAATACAAAATTAGCCGGGCATGGTGGCACATGCTCGTAATCCCAGCT
ACTTGGGAGACCGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCGAGATTGCAC
CATTGCACTCCAGCCTGGGCAACAAGAGCAAAACTCCATCTCAAAACAACAACAACAACAACAACAACAA
CAACAACAACAAAAAGAGTACAAGGTTTAAATTTTTTTTCCTTTTTACTGATTCAGGTTACTTTGGTGTC
ATTTTCCCCCAACTTAGATAAACCCCTAATCAATTGTTTCATTGTAAAGAGGAATATTTAAATGGTACCC
CTAAAAATTCCTTTATTATTTTATTTTTGTTTTTATTTTTACTTTTTTTGAGATGGAGTTTCACTCTTGT
TGCCCAGGCTGGGGTGCAATGGCGTGATCTTGGCTCACCACAACCTCCGCCTCCCGGGTTCAAGCGATTC
TCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAAGCATGTGCCACCACGCCAGGCTAATTTTGTATTTT
TAGTAGAGACTGGGTTTCATCATGTTGGCCATGCTGGTCTCAGACTCCGGACCTCAGGTGATCTGCCCGC
CTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCCCAGAATTCCCTCTTTTAAA
CAACAAATTGTACTTTTCCCAGTTTTTTGTTTGTTTGTTTGTTTTTTGAGATGGAGACTCACTCTGTCAC
CCAGGATGTAGTGCAGTGGCATGATCTCGGCTCACAGTAACCTCCGCCTCCCGGGTTCAAACGATTCTCC
TGCCTCAGCCTCCTAAGTAGCTAGGATTACAGGCACCTACCACCATGTCCAGCTAACTTGTATTTTTAGT
AGAGACGGGGTTACACTATGTTGGCCAGACTGGTCTCAAACTCCTGACATTGAGTGATCCACCCACCTTG
GCCTCCCAAAGTGCTGGGATTACAGGCATGAGCTACTGTGCATGGCCATATTTCCCCAATTATCCTATTA
AGTAAGAGTCCTAGCCCTAAGATCTTAGAAAATATTGTTTAATTAAATACCAGGAAATACCTCTTACTAA
AAAGCCTAGTCTTAAACAGCTTGAATAGAAAATTCCTTCAAGTTTTTTATTTTTATTTTTTTTTGAGACA
GAGTCTCACTCTGTCGCCCAGTCTGGAGTGCACTGGTGCAATCTCGGCTCACTGCAACCTCTGCCTCCTG
GGTTCAAGCGATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCACCACACCTGGC
TAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCACATTGGCCAGGCTGGTCTTGAACTCCAGACCGC
AAGTGATCCACCCACCTCAGCCTCCCAGAGTGGTGGGATTACAGGCATAAGCCATCCCGTCCAGCCCATC
TTTTTCCTTTCTGACTTCCTATTTATTTTTATTTTTTATTTTTTTTTTGAGTCAGAGTCTCACTCTGTCA
CCAGGGTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGTGATTCTCC
TGCCTCAGCCTCCCAAGTAGCTGGGATTATAGGCACCCACCACCACGCCTGGCTAATTTTTGTACTTTTA
GTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTTGTGATCTGCCCGCCTCA
GCCTCCCAAAGTGCTAGGATTACAAGTGTGAGCCACTGCACCCGGCCCCTTCGTATTTATTAAGATAAAG
AAAATTGCTGGCCGGGCGTGGTGGTGGGCACCTGTAGTCCCAGATGGGAGGCTGAGGCAGGAGAATCGCT
TGGACCCAGGAGGCAGAGATTGCAGTGAGCTGAGATCGCTCCACTGCACTCCAACTTGGCGACAGAATGA
GACTCCGTCTCAAAAAAAAAAAAAAAATTGCTCAGGCTTTCCTTTAGAGGTTTATTTGGAGATAAATGGA
AAAGGTGTAAAGTATTTATATTAATATTTTCCCTTGGAACAAGTGTATCGAATTGAAAATATTTTTCTAA
CTCTTTCTTCCTACCCTGACAAAAGAAAGTAAAATCAGATTCTTTATGGTAGGTAAGGAGAACACCATTA
TCTTTTTAAAGATGTGGGATTTCCCTACCAGAATTGTAGAATATACTTGTTTGTTATTAGACTATGTTAG
ACATGTCATCATATTGAATTTGGCAAATTTTCTTACAGAAATCTGAAAAAATTTTATATTCTGTCCCTCT
AGTTTTATCCCTGTCTACCATCTTACATTATCTTCTCCCGGTTCTCAGTACAACAATGAGCCTGGACCCA
TGGTTGTTTCCTGCCTGTAAGTCATTCATTCTGCTATTTCCCCTACCAAATGTCTTTCTCTTCATTCTCC
ATCTGCAAAATATTGTAGTCCTTCAAATCTCAGCTCTTAAAATAAATTTTCCTCATTTCTCCAAGGTAAG
AGCACTTCCTTTAAATCCACTTAAGTCTGTGTGGATTTTCTTTTCCTTTCTTTTCTTTCTTTTTTGTTTT
TATTTATTTATTTATTTATTTGAGATAGGGTCTCACTCTATCATCCAGGCTGGAGTACAGTGGCGTGACC
TCAGTTCACTGCAACCTCCCAGGCTCAGGTGATCCTCCCACCTCAGCCTCCCAAGTAGCTGGTACTATAG
GTGTATACCACCATATCGGGCTAATTTTTGTATTTTTTGTAGAGACTTAATTTCGCCATGTTGCCCAGGC
TGGTCTCGAACTCCTGGCCTCAAGTGATCCACTCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATG
AGCCGCCGGGCTGAGCCAAGTCTGTGTTTTTATTTTATTTATTTATTTTTTCTGAGCGAGACGCAGTCTC
GCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAAGATCTCGGCTCACTACAAGCTCCGCCTCCTCGGTTCAC
GCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCACTACGCCCGGCTAATTTT
TTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTAGCCGGGATGGTCTCAATCTCCTCACCTCGTGATC
CGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCTACCGCGCCCGGCAAGTCTGTGTATT
TCTTAATGCACAGTTATTTGTTAAAATGTTAAATTATGCCCTAATGTAGTGTATGCTACTTGAGGGAAGG
ACTTTCTTACTCATATTCATATTTTCCATAACATGCAGCACTATGTATTTGATTTCCAAGATATTGGATT
GATACTTGTCCAATGAATGAATGAATTAAAAAATATAAGGGTCAATTCTAGGATTATCTTTACAAATGGC
TACTCTAAAAATGCTCTGTAAATGTCTCTATTGCATGTAGGTGGGATAAGCCTAAAGTTAGTTCCAGAGG
CAGGGACAGTTCTGAAGGACTTGATAAAAATCACTCACAGATTCTGCACAGACAAGGACACTCCCAACGT
GAATAGATAACTTGTTGGATGCATCTGCCAGCATCTGAACTGCTGGCAACTTTTGTTTAGAATGAAGAGC
TTTCAGCCCAGCACCTCGCCTCCATTATAATGTTTTGCCTCCTGTTTTGTTTATATAAAAGACTACAGTC
TTGTCTTAGAATTTCTGGGTATTATGAGTACCTTAGGGAAATCTGATTATAGAGATAATCTACTCAACAG
TGAAGAGTTTCAATTTGTTTCTATTGAGTTTTGTCAATTCCGAATCCCAGATACCTTTGTATTCCAGTCC
ATTGACTAGAGCCTCCCAGCCAGACGTTGAGGCATCTGTTTCTATAGTCTCCGGAACTGGAAAAGATAAA
TAAGAATGTAGGGGAATTATCTGTATATTTGCTAACCACCAATTTAGATCTAAATAGACTCCCTCTGTTA
GGAAGACTAATGAATTCCAATTCTCTGAAATATCCTTTGCTTTTAACAAGCAAGATGGAAGGTATCTAAA
GAAAAAAAACAGGGCGAGATACACAGAGGGAAAAGCTGTGAATAATGCCACCAGAGAACCAATTATGTTC
ACCAGCTGGCATTGAGAAACTTGTTTCCTTTGACAAAAAAAGATTTTAGTTTTAGCCTCAAAATACTTTT
TGACATTGTGTTCTTCAGGGCTGAATGACGAATCCTCTCAACCACCCCCATAAAGTGTTAGAAAAAAATT
CTTAACATCATTGTTATATGTAATGTGGCAAAGCAATGGGCAAGAAGAGAAAAACAAGCTAAGGCATCAA
TATGGCAGTTAAGAAGAATCAACTGAATAAAATGATAGTGAAATGAAACATGAAAACAGAGACCTTCCTC
CCAGCCATATGACTGAATCAATGAGGCACATTCACGGGGTATGACAGTGATAAACAAACTGGTAAGAACA
TCGAATAGATGTAAAAATGTATTTCTTGTGAAAGCATAGTCAACTACTATATATCTCAGTGATATAAACT
GCATTTTGAACTTTTTTTCTTTCTATTTGTGGGGAACCTCATTGATTGCAACCAAGCCTAGAATGGAACC
TTTTGTGCTTTAGTGGGTGAGACATAATCTATTTCATGATCTCTCAGTTGGTAAAATTTGCAAAGAAGGC
TGGGCACGGTGGCTCACACCTGTAATCCTAGCACTTTGGGAGGCCAAGGTGGGAGGACTGCTTGAGTCCG
GGAACTTGAGACCAACCTGGGCAACATAGCACCACCCTGTTTCTACAAAAATTAATAATAATAAATTAAT
TAGCTAGGCACGGTGTTGCACATCTCTGGTCCCAGCTACTTGGGAGGCTGAAATGGGAGGATCACTTGGG
CCTGGGAGGTTGAGGCTGCAGTGAGCCATGATTGTGCCACTGCACCCCAGCTTGGGCAACAAAGCAAGAC
CTTGTCTCAAAAAAAAAAAAAAAAAAAAAAAGCCAAGAAGTGGTGACACCTAATCTCGAGCTCTCATTAG
AGCTTTTTTTTCAGCTACCAGGACTCCCGTTGAGCCAGTCTTGTCTGGGAGTCCCCTGTGGTCCATCTGC
TCCAAGTTGTTCCTTGGGGAAATACTTGTGAATTCAAGGTTTTCACTTTTTATACTACTTTAAAACTTGC
CTTTCCCAAGTATCTATTTAAATTCCATTAATATCTGTAGAACAAGTTCACTCAGACCAGGCTTTGTTGA
ACCACTAAAGCAGGGCGACTATGTTTCTATGTGCTCCGAATGCCTTCTCTTTCTCAAAGAGAAAAAATAA
TGCTTATTGTTAGTAATTTGCTTTCTTGATTATAAAAAGGGGAAATTTAAAAAAGTTTCTTGTTTCTCTT
TCAAATGATGCCACCTGGCGCAGAGCCACTGTGTAGTCTCTGTTCTGCACAATTCCACGAGCACCATCAA
CATGGACTATAATACCAGTGGTGCCTTCTGACCCCCTATCCAACCTGAATTCTCCAGAGTAGTACATAGT
CTTGACCCTGTGCACAAAGAATATGGATTTTTATGTCGCTGGATAATTGGACAATTAATTTCGTATACCC
AGTTCTGGGGAGGAAACGAGAGCTGTTATTTGATTTCATGCCATCAACTTCCTAAACACTTTCATTTACT
GAAATTTGGTATTGTACTAAATATTACAGAAATACGAATGTTACCTAAGTGACTCTTGAGCTTTGAACCT
AAATCAGCAAAGGAAATGTTTTACGAATTTGTTTTTTTTTTTTTTTTTTTTTTTTGAAACAGAGGCTCTC
TCTATCACCCAGACTGGAGTGCAGTGGCGCCATCTCTGCTCACTGCAACCTCCGCCTCCTGGGTTCAAAC
GATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCTCCCACCACCATGCCCAGCTAATTTTTG
TATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTTACACATTTTATTTCCTATTTCTT
CATCTTGTGTTTTTTATCCTTTTATTCTCTTTTAAGGATGCAGTTTTTAGTGATGGATTATACATGGAAA
GAAATGAAAAAAAATGAGTTCAAGGAGAGTTCTTTAATAGTATGCATCAGACTTCTTATAAATTTACTGG
AAATGTCTTAAAAAACAATTGCCCTTTTCTTTATTAATACATTTTTTTAAATAGCAGGTTTTTTTGAGAT
TTAATTCAACCACAAAATTCACCAATTTAAAGTATACAATTCAGTGTTTTTTAGTATATTCAGTTATGCA
ACCATCACCACTATCTAATTTCAGAACATTTTTATCACCTCAAAAAGAAGCCCTATATCCATTAGCAGTC
ACACCTCACATTCCACCCCCAGCAGCCCTAGGCAAACACTCATCTACTTTTTGTTTCTATAGATTTGCCT
GTTCTAGACATATTATATAAGTGGAATCATACCATAAATGGCTTCTTATGACTGATTTCTTTCACTTAGC
ATGCTTTCAAAGTTCATATATGTTGTCACATGCATCAGTATTTTATTACTTTTTATTGCTGTATAATCGA
TTGTATGGATATATCACATTTTGTTTATTCACTCATCAGTCGATAGATGTTTATATTGGTTTCACTTTTT
AACTATCACAAATAATGCTGCTATGAATATTTTTATACAGGTTTTTTGTGTGGACTTATTTCTTCAGTTC
TCGTGGTTATATACTTCTGAGTAGAATTGTTGCATTATATGATAAATATATGTTTAATCTTTTAAGTAAA
TATCAGACTGCTTTTCCTTTCCTTTCCTTTCTTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTT
CCTTTCCTTTCCTTTCCTTTCCTTTCCTTTTTTTCTTTTTCAGACAGAGTCTCACTCTGTCACCCAGGCT
GGAATGCAGTGGCACGATCTTGGCTCATTGCAACCTTCAGCTCCCGGGTTCAAGTGATCTCGTGCCTCAG
CCTCGCAAGTAGCTGGGATCACAGGTGTGCGCCGCCACACCCAACTAATTTTTGTATTTTTAGTAGAGAC
AAGGGTTTCACCATGTTGGCCAGCCTGGTCTCAAACTGCTGACCTCAGGTGATCTGCCTGCCTCAGCCTT
CCAAAGTGCTGGGATTACAGGCGTGAGCAACCGAGCCCAGCCCAGACTGGTTTTCAAACTGACTGAATCA
CTTAATAATCCTGCCAGCAATGTTTGAGGGCTCCAACTTCTCTACATCCTTGCCAATATTTGTTATTGTC
TATCTTTTTAATTTTAGCTGTCTTAGTGAGGATACAGTGGTATCTCGTCGTGGTTTTGATTTGCAGTTCC
CTGGTGACTAATGATGGTGAGCGTCTTTTCATGTGCTTATTGGCCATTTGTATATCTCCTTAGGAGAAAT
ATATAGCCAAATCCTTTATCCACTTTGTATTATCATTTTTACTTGTCATAAAATACACAACATAAAATTT
ATCATCTTGACCTTTTTTAAGCATACAGTTCAGTGGCATTAAGTACATTCACATTCTTGTGTTACCATCA
CCGTGATTCAGTCTCCAGAACTCCTTTCATCTTGCAAAACTGAAACTCCTTTTTTGTTTTGACCATTCTT
TCCATCTTTTATTACTACTGAGGTTAAACATTTTCATGTGATTACTGGCCATTCATATTTCTTTGTGGGA
CAGCTTGGTAACTTAAAAAAATACTAATGTTTTTCCTGATTATAATATACTTATAGATATGTAGAAAATA
CAGAAGAATATAATACTATAGAGGAAAAAATTCACGTAATTCAAATTATAAAAGCAACCATCGCTGCTAA
TTTTTACTTTTCTCTGCACATGTTTTCTTATAGAATTGATATTCTGGACAAATGTGTTTTAGAGTATATG
TTTTTTCTTTCCTTTTTTTTTTTTGAGACAGAGTCTCGCTTTGTTGCCCAGGCTGGAGAGCAGTGGCTCA
ATCTTAGCTCACTACAACCTCTGCCTCCTGGGTTCAAGCGATTCTCCTACCTCAGCCTCCCGAGTAGCTG
GAATGACAGGCACGTGCCACTAAGCCCGGCTAATTTTTGTATTTTTAGTGGAGACGGGGTTTCATGGGCC
AGGCTGGTCTTGAACTCCTGACATCAGTTGATCCTCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAAG
GTGTGAGCCACCGCACCCGGCCCTGTTTTCTCTTTTTTGTAAAATTTAATTTAATTTTAAGTTCCAGGAT
ACATGTGCAGGATGTGCAGGTTTGTTACATAGGTAAACGTGTGCCATGGTTTGCTGCACCTATCAACCCA
TCACCTAGGTATTAAGCTCAGCATGCATTAGCTATTTTTCCTGACGCTCTCCCTCCCCAAAACCCCACCC
TGACAGGCCCCAGTGTGTGTGGTTCCCCTCTCCGTGTCCATGTGTTCTCATTGTTCAGCTCCCACTTATG
AGTGAGAACATGTGGTGTTTGGTTTTCTCTTCCTATGTTAGTTTGCTGAGGATAATGGCTTCCAGCTCCA
TCCATGCCCCTGCAAAGGACATGATCTCATTCTTTTTTTTATGGCTGCATGGTATTCCACGGTATATATG
TACCACATTTTCTTTATCCAGTGTATCATTGTTGGGCATTTGGGTTGATTCCATGTTTTTGCTATTGCGA
ACAGTGAAAACTGAAACTGTTTACCCATTCAACGATAACTTTCCCTTCCTTCACCCCAGCAACTCATTCT
GTTTTCAGTCTCTATGAATTTCAGTACTCTAGGTACCTCATTTAAGTGGAATCAGGCAGTATTTGTCCTT
CTGTGACTGGCTTATTTCACTTCCCATCATGTTCTCAAGGTTCATCCATGTTGTAACATGTGTCAGAATT
TGCTTCCTTCCTTTTTTTTGAGACGGAGTATCATTCTGTTGCCCAGGCTGGAGTGCAGTGGCACAATCTT
GGCTCACTGCAACCTCCACCCCTGGGATCAAGCAATTCTCCTGCCTCAGCCTCCCTCGTAGCTGGGATTA
CAGGTGTGCGCTACCACTCCTGGCTAATTTTTGTATTTTAAGTAGAGACGGGGTTTCACCATGATGGCTA
AGCTGGTCTCAAACTCCTGACCTCAAGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGC
GTGAGCCACCGCGCCCAGCTGTAGAATTTCCTTCCTTTCTAAGGTTGAATAATATTCTAATTTATGTATA
GTCCACATTTTGCTTATCTATTCATCTGTCGATGGACACTTGGGTTGATTCTACCCCTTGGCTATTGTGA
ATAATGCTGCTATGAACATGTGTGTATAAATACCTGTCCAAGCCCCTGCTTTCAGTTCTCTTGGGTGTGT
ACTCAGAAGTGGAATTGCTGGATTATATGGTAATTTCATCTTTAACTTTTTAAGAAAGTGCCATACTGTT
TTCCACAGCAGCTGCACCATTTTACATTTCCACCAACAGTGCACAAGGGTTCCAATTTCTCCCCATCCTC
ACCAACACTTGTTATTTTCTGCTTTTTAAAAAACAGTCGCCATTCTAATGAGTGTGAGTTTGTATCTCAT
TGTGGTTTTGATGATTTGCATTTCCCTAATGATTAGTGATTGTATTAGGAAGTTTGGGCTGCCGTAACAA
ATACCACAGACTAGGTGGCATAAACAACAGAAATTTATTTCTCACAATTCTGGAGGCTGGGAGTCCAACA
TCAGGTTCTGGCTGATTTGGTTCTTGGTGAGAGCCCACTTTCTGACTTTCAGATGGTCACCTTTTCACTG
TATACTCACATGGTAGAGAGAGATCTTTCTCTCTTCCTTGTCTTATAAGGCCACCAATCCTATGAGATTA
GGGTCCTATCCCTATGACATCATTTAACCTTAATTACTCCCTATAAGTCCTGTCTCCAAATATGAACACC
TTGGAAGTTAGGGTTTCAACATATGGGTTTTGGGGGGACACCGTTTGGCCCATAGCAGTGACCTCGAGCA
TCTTTTAATGTGCTTATTGGTCATTTGTGTATCTACTTTAGAGAGATGTCTATTCAAGTCTCTCACCCAT
TTTTATTTTTATTTTTATTTTTTAGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTAGCAGG
ATCTGGGCTCACTGCAAGCTCTGCCTCCTGGGTTCACTCCATTCTCCTGCCTCAGCCGCCCGAGTAGCTG
GGACTACAGGCGCCCACGACCACGCCCGGCTAATTTTTGTATTTTTTAGTAGAGACGGGGTTTTGCCGTG
TTAGCCAGGATGGTCTCCATCTCCTGATCTCGTGATCCACCCACGAGCCTCAGCCTCCCAAACTGCTGGG
ATTACAGGTGCGAGCCACCGCACCCGGCCTCGCCCATTTTAAAACAGGGCTGCTTGTCTTTTGTTGTCGT
TGAGTTGTGGGAGTTCTTTATGTCCTGGATACAAGCCTCTTAATAGATATGTGATTTGCAAATATTTTCT
CCCATTCTGTGGGTTGTCTTTTTCATTTCTTGATGGTGTTCTTTAAAGCACAAAAGTTTTTAATTTTGGT
GAAGTCTCATTTATTTTTCTTTTGTTGTCATGTCTAAGAAACCATTGCCTAATTCAAAGTCATGAAGATT
TACTTACTCCTATGGTTTCTTTTAAGAGTTTTATAGTTTAAGCTCTTACATTTGGGTGTATGATTCATTT
TGAGTTAATGTTTATATACGGTGTGAGATATCCCTTTTTAAAAAATTCCTAAGAACATCTGAGTTTCAGA
ATGTTCTTTACTAGATTAGATATGAAAAAAATTGGTGAGGAGGGTTTTTTTTTCTTTTCTCTTTTTTTTT
TTTTTTTTTTGAGACAGAGTCTCTCTCTGTCACCTAGGCTGGAGTGCAGTGGCACAAACATGGCCCACTG
CAATCTTGACCTCCTGGACTCAAGCAATCCTTCCACCTCAGCCTTCCAAGTAGCTGGGACCACTAGGTGT
GTGCCACCACACCTGGCTAATTTTTAACTTTTTTGTAGAGACAGAATCTCACTGTGTTGCCCAGGCTGGT
CTCAAACTCCGGGGCTCAGGCAATCCTCCTGTCTTGGCCTCCAAAAATGTGGAATCACAGGGACGAACCA
CTGTGCCCAGCCAGGAGGGTTTTTTAAATTGCTGTTTTGGGGGGTCTTTTAAAAATTTTATGGACTCATT
GCAGACATGGGAAATCACGTCTATTATATCATTTAAAATATGTTTCATTTATAATAAGCTCATTCAGTCT
GTTTTATAAGCAGAGAAATCCAAACATACGGTAACTTAACAGGAATGGCTGCCAATAACAGTTGAGCTAA
CACTCATTGAAAGATTATGAATAATATCTTGATTACAAATCAAAGCTTGGTCTCATCTTTTTAGCCCAAA
ATTACTAAGACAACGTAACATTTTTTAGTCTGATTAATGGTAGAGTAACTAATTAGAGTGGGAAAGAGAT
TAGCAAATCATTCAAGATGTCAAGGGCAGGCAAAGTGCCGTTTTGTCATAGATAATATAGTAAAGCTCAT
AGCGACAGGTGTGCATAATAAGTAAGAACTGAGAATGCCTAGGTATTGAAGAGTCTAGGTAATGTTTTTA
ATATCAGCATTATTTGGTTAGATTTGATTTTCTTCTTCTGAATTTCCTTACTGCTGAATTGGCCTTTGAT
TTATTGACATTCTAAGATTTGGAGTGGATTTTCTTTTTGCCTTGCAGGATGCAGAGAATCAAATATTAAA
GACAAGTGTATGGTACCAAGAGGTAAATAATTATGTTTTCTTCTAAATATATTGCACTCCTGATCTGGAT
CTGCTGCAAAATATAGTTATTTAAGAAATAGGTATTATCTTTGTATTTATAGTTTCTTAGATCTGCAGCA
GACCTAATGTATTATAATTCATTTAAGATGTATGGCCCTGGTTTCTGGTTGGGACTACCATCTTTAGGAT
TCAGATGGGAAGTCCTTTCTCCTAACAGGTAGAACCAAGAGGCTGATAAATTGGATTATTAATCCAAATG
CCTCTTTAGTTCTTTAGCGAAGTAGATATTGCCCCATTTTGATAAGTTTTCTTTTTGGCTACTACTAACA
GGTCTGGAATGATGAATTTTTATCCTGGAACTCCAGCATGTTTGATGAGATTAGAGAGATCTCCCTACCT
CTAAGTGCCATCTGGGCCCCCGATATCATCATCAATGAGTTGTAAGTGTGCCAGTGTGTATTTCTGTGGG
GTTTAGACTGCTTGGTATAGTCGGGCCAGTTGCTGCTTACTTTCAGTCAGTGTTGCTCATTTTTGCTTCT
CAAGTTGAAGGGGAGCATATTGTTGGGAGTATTCAGGTTAGAAGTGCCATGTGTTGACATTATTTAAGAT
ACAGATCTGGTTACTAATGTTGAGGTTACTCATTATGTCTGTGTTGTTATAGCAGCAAATGAGTTAACTT
ACGAGGCTGTCACTGAAAAGCTCATCTTTGCCAGGGTGAATCATCTCATGGAAAATGCGATTCTGTTTTG
CAGGGCTAGGCTGGTCCTGGACCTCATGGTCACTACCATCTCCTAATCAGCCTATGTTTTGAAATGACCA
ACATCCTCTCTGTGACAACAAGTTCTCTTGTGTTTCATATAGTGTGGACATTGAAAGATACCCTGACCTT
CCCTATGTTTATGTGAACTCATCTGGGACCATTGAGAACTATAAGCCCATCCAGGTGGTCTCTGCGTGCA
GTTTAGAGACATATGCTTTTCCATTTGATGTCCAGAATTGCAGCCTGACCTTCAAGAGCATTCTGCATAC
AGGTAAACCATGAGAGATACCCATTAATGCTAGGTTGGTGCACATAGGTGAAATGATATTATACTATCCT
TCAGGTCTATTTTATTCTTGCAGATAATTGGCTATTTAAAAATTGGAATCTCTTCTTGCGGTTTTTTGGC
TCCTGCTGTAATCCAGACCAAGTATGATGCTGTAGGCCCCCATAGTTGTTTTGGAAACAGTGTTAGGCCT
CTACTGCCATGGATAATATTATAGAAATCGCAGGCAAGCTCCATCATTCTACAGAGAGTGTATTCCAAGC
CATAAATTCACAGTTGTTGTTTTTTTATGACTAGAAAGATTTTATTCCACATCCCTACCCCCTAATTCAA
ATAAGGCCAAGGAGACTGTGCCTATGGGGGCAGGAGAACGAGGAAGGATTCGAATTGGGAGCTGGAAAAG
TGGGTGGATGTTGGCATCTCTTTCTCTCTGGGAAAGTCAATTGTTTGTGTTGTTTGCAGTGGAAGACGTA
GACCTGGCCTTTCTGAGGAGCCCAGAAGACATTCAGCATGACAAAAAGGCGTTTTTGAATGACAGTGAGT
GGGAACTTCTATCTGTGTCCTCCACATACAGCATCCTGCAGAGCAGCGCTGGAGGATTTGCACAGATTCA
GTTTAATGTAGGTTCTTTACTACCTGTCCCCGTTGCCCGCTTCTCCCCAGCCTTTGGCCTTCTCTCTTGG
GCCAAGGAATTTCTGCTCTATTGCATGTTCTCATTCATTATCACCCAAGAACAGGGACTTCAGCGGGCTC
TGAGCATCCACCTGCATTCCAGCATTTCACAGAGCAGAGGTTCAGTGGGAGATGCTGGGCCCCTCCAGAA
GATAACAGACACCAGAGGCCTGGTATTCAGACACCAGAGGCCTGAAGCATGCCCTGCAGCAGGAATCCTC
CAATCTTTCCACACACACTTACATTATGGCTTCTAATTGCTACTCAGAAAAAACACAGACAAACCCACAC
ACAACAACAACAAATTGGGAAGACTTTCTCATCAACATTATTGTCCTATTTTTTTTTTATTGAGTAAATT
ATCAACTAATTCCAGATGCAGATGCATAATACGATAATATTATTAACTTGAATATCTTAAGGGATTAGCC
AATAAGACCCACAGAGACCACTGGTAGCTTCTGGATCAGAGGGAACAATTAACCCCCTCAAAATTATGAT
GGGAACCCACACTCCTCCGATGCAGCCCTTGGCTGCATTGTTCATAGGGAAATTTGTCCGGCATGAAACT
AGGGGATGGTGGGGTACAGGACAGTGAAGCCCGCTGATTTGTACTCCAGAGGCAGCCATTGTCCCATCTT
TGGCCCCCTGGGAAACTGTTTTGACACATGGTCTGCAACAATTTCACACAACTGGTAAACTACATCATTA
GTATTCAGCAGTGCATTCTTCTTTTCTGCAAAAAGTCAGCTAGTGGTGGTGGTAGTGAGGGAATGGTGGT
CCTTCATGCCAAGTAATGGATTCAGTTGAAAAATAGAAACCACAGGCAGGTCAGGACTGCACACAAGATG
AATTATCCTAAAAATATACCTTTGGAATAAGAAGGAAAACTTAGACTAGACTAAGGTGCTCTGGTACAAT
GGATATGCTAAGGGGAGGTGAAGAAATAAACTATCATTCGCTGAGTGCGGTGGCTCATGCCTGTAATCCC
AGAACTCTGGGAGGCCAAGGCAGGCAGATCATTTGAGGTCAGGAGTTCGAGACTAGTCTGGCCAACATGG
CAAAACCCCATCTTTACTAAAAATACAAAAACTTGCTGGGTGTAGTGGTGTGTGCATGAGAATCGCTTGA
AGCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATACCACTGCGCTCCAGCCTGGGTGACACAGGGAGA
CTCTGTCAAAAGAAAAGAAAGGAAGGAAGGAAGGATGGAAGAAGAAAGAAGGAAAGAAAGAAAAAAAGAA
AGAAAGAAAGAAGGAAAGAAAGAAAGAAAAAAGAAAGAAGGAAAGAAAGAAAGAAAAAAGAAAGAAAGAA
AGAAACTATTATTGAGGTTACAGGTATCCCTTAATTGGTTAAAGGTGTATACAGCTTTCCAGCAAAGCTC
TTGTTAAATCTGGGCCTCTTAGACACAGTTGTGGTTTGAACCTGACTAATGTATAATGCTGGCTAGTATA
ATCGCAACTCATACCCTCCTGATGGTGGGCTTTGGGCTCTTGCCTAGAATCCTGCTTGGTTTTAAGCAAA
GCTTGCATTGTGGCCTGTTTGTCCACTTTTCTTGTAAGGAAAAGGGAAGGAAAGCAAGCAAGCTTTTTTT
TTTTTTAAATTTCAATGAGTTTCTTGGCTAGGAGTAAACACCTCATTAATTTTAAAGATATCAGCCCACA
AACATTATTAATGCAGCCCGAATAATGGAGAGCATTAAAGGGAAATGTGAAGGAGTAGCACATGCAAGGT
GGAACATAGTCACCTACCAAGTGCTCCTGAGAACACTGCACACACCATTACCTACACTGCACGGAGGAGA
CAAACACAGGAAAGAGATGGGGCAGGTAAACAAGGGCGTGTGCTTGCATACACAGGCACACATGTGCACA
CACACACACACACACACTCTCTCTCTCTCCATAGAGCAGCTATTATTTGTAGTTACATGTCACTTTCCTT
CACCCTAAATGTTAATTCGTCAACAGCAGCTGAAGGTTTTCTATATACCATCTCCATTTTAATTATGATG
AACAGAGACAGAAGATTGTCTAATGGAGGGGAGGCTTCCCTGGATCTCAGAGCCATGGTCCAGTTCCTCT
GCTCTCCTCACTGCCATGCTTCCCCCCGGGTACGTTTTGAAGTGGAAGTGTGCTGGAGGCAGGTGCAATT
TGATTTCATGCTCTTGGAACTTTTCCATGGGTCCTTTTGCCCCGGGGGTGGACGTGGGTTATCTAGCAGG
GCTGGCCTCTGCCCTGGTCCTCAGGAGCAGAGTAGGGAGAGAATCCTGAGTAGAGTCACAGATCATATTT
GGTGGGACAGGAGGCCAGACTCAGGAGCAATTCTGCAGCTTTAGAAAGACTAAAAGTGGATTCAGTGACG
ATCACAGTTTTCTGCCTCCCAACCTCGCTCAGCCTCCATGCATTTCTGCTCTGTGCACAGAGCCTAGGCT
CATTCTCTTACTCCATACCAGACATCCCACTCTGAATTTAGCAACCAAGGCACCTGGCTTTCCTTCAACA
GCCCTTGGCCAGTGATGTGAAATTCAGAGCCTGATTCTATTATGCCAACACTGGGCCTGCCAGGGTCCTG
ACAGCAAAGGACCATCTCCTCTTAATCTACTCGCCGGCTGTGTCTCAGTCCAGGCTGCCCAGCGGGAGGT
TGGGGACGGGTAGGGAAGTCAGGAGAAGCAATAATCTATTCTAAATTCATCCTTGCCCTTGGCCTGAGGT
ACACTGCTGCCATTTATACTAAATCAAGTGCTCCGGATGCCCCATGAGTTTCAGCTAATGTGCAATCATA
GCTGCCGACTCTGGGACACAAATTGTTTATCAGAGGACACCACGGATACTTTGGAGTCAGGCTACATTCC
ACAGGGGGCCCAGAGCATAGGCACTTCTCCCTACAAAGATGTCTGTGTACAGCCTGGGACCCGGCCCAGC
AGCCATGGGAGGGGAGGTTTCCTCCAATATATTATTGGAGGTGCTGGTTCCCAGAAACAGCAGGGTGAGA
GCATTTGATTTTGCATGGCAGTGGTTTTTTTCTTTTTTTTCTTTAAAGAATGAGGGTAGAAGTGGGAGGA
GGTAGGAATACAATTTAGGAACTCGTGTTGGATGGAAAAGGGTGGACAGCTGTGGAAACTAAACCGAGCC
CAACCACAGATATCCTCACACCAAGTAAAGTGTTTATTCGGGCAGGAATTGGGGTGCGCACAGGGTGTCC
TAGAAAATGCAAAGCGGGCATTTGGGAAAGAAAGGTGCTTCATTTATCCTCTTTTTCTGTTATAATTTTT
TTTCTAGATTAGGTAGAGTAACTTTGTTGAGATACTTATTGAATGCAGCTTATAAACTTGATAGAAAATC
AAGATCCCTCTAAGTGCTAGCAGATCATATGAGAGAGAGAGAGAGAAAGAGAGAGAGAATTTAGAGCCCT
GGATATCCTTTCTGGGTCACTCAAGTGATGTGGGAATCACAGACTGACTTTCTGCTAATCCAGAGCGACC
CATAGTCTAGATCCAAGTAACTCTGACTCCGGTGCTTAAATGGCAGCAACTTTCCTGTTCTTTCTGAGTT
GGGAATGCTGGGAGGGGCTTTGGAGAGGAGGCAAATGGGTTCTTCTGTATGAAGCGGAGTCTAGGCGTCA
AAATTCCAACTGTGTCATAAAGACGGTCATGCAAAACTGAGTACTGGTGGGGTCTACCATCAAGGAGGCA
ATTACAGGCTAACCTTGGGGCCTATTCCATCTCCAAGGAGCTCTCCACACACACATTCGGAATTAGATTG
ATCTCTTTCATTAACATCAATAGGCTCCAAATACCTTTGTGTACTTATTATTACCTGGCTTTAATGGATA
ATGAAATTGTGGCATAATATTGCTAACACCTGAAGGGTGTCTAATGGGGAACATGGACTGAAACTAAAGT
GCTTTTTCTTCCCTTTCTGTGGAATTTAAAGGCACCAGCTCCTTTAGTCCATTAGAAATGATGAGCTGTA
GGTAGGGGCAGTTTTGCAGAGAGCAGTGTCTCACTGAGCAGGTGACATGCCTTCTACTCAGCAGAAGCAG
GAGTCACTGCTTCTTGCATGATCTGCAGAGAGGTCGCCCTCAGAAAGACTGACCTCAAGAGGATAGCCTC
CATTCAGGCTAACTCCACACTTCCATTATATTGGAACTAAAAGTAGTGCTACTACCAGACTCCAGTACAA
TTCAATAAACATCTTTTGAGTTCTTACCATGTGCCAGGCTCCATGGTGAACACTTAGGACATAAAGATGA
ATAAATGAAGGCTCCTGACATCTATCACAGATCCCCAAAATCTGCCCAGGGAGACATAATAGCAATAACC
ACAAAGCTGAGCACTTAGCCTATGCCAAGCACCATTTTAAAGTTTGTACCTATAATCCTCACATCCCTAG
GAGATGAGTGATCCAAGTATCTGCATTTTTACAAATAAATGAATTGAGGTGCAGAGAAGTTAAGTAACTT
ACAGGAGGCTGCAAGGTGACTGGTGGAGCCTGGACTTGAACCCAGGCAACTTGACTCCAGAAACTATGTG
CCTAATTCCATATCATGCTGACACACAACTAAAGGCAGTGCAGTATATTCTGGTCCATTCTTTGCCATTT
GCCCACTGGGCATTACTTGGCAGGTCGCGTGTATTAGTGTCACAAGGCTGCCATAACAAAGTAGCCCAAA
CTGGGTGGCTCAAAACAACAGAAATTCATTTTCTTGCAGTTATGTAGGCTGTAAATCTGAAATCAAGACT
TGGGCAGGGTTGGTTCCTTCTGGGCATTCTGAGGGAGAATCTGCTCCTTGCCTCTCCTCTGCCTCTGGTT
GCTGCCAGGAACCTGTGGTGTTCCTTGGCTGGTAGATGCGTTGCTCCCGTCTCTGCCTCCATCTTCACAT
GGCCTTCTCCTTGTGTGTCTCTTCTCTTCTTATCAGAACTCCAGTCACATTGGATTAAGGGCCCACTCTA
CTCCAGTATGACCTCATCCTACTCTAGTACATCTTCAATGATATCATTTCCAAATAAGGTCACATTCTGA
GGTTCCAGGAAATACATGAATTCTTGGGGGCCCTCTTCAACCTAGTAGAGTCTGCAACCCAAAATTCACT
TCTGCTCCATGTGCAAATTCATTCAGCCGCATCCCAACATCCCCCAAAGTTTAACCCAGCATCATTCCAG
TGTCAACTCTTAAGTCCAAAATATCATCTAAATATAAACTCGAAAAGTCCCAAATCTCATTGTTTAAATG
ATCTAAAGCAGGTAATGACCTTGATTATGGTCCATTCTGGGGCAAAATTTCTCTCCTGCAACTGACTCAT
GAAACACTCAATCACCAGAATCTGCTGCCTGATCCTACAAGATGGGATAAGGGCAGGGAACTGTCTTATT
AATAGTACTAACTTCAGTATTTAGGATAGTACCAGACAACCTGTTCATGCTCAATTAATGTTTATTGAAC
AAAGGAATAAAGGGCTCTGATTATCCAATGAGATAATAAAAACAAAAAATTTCTGAAAACTATATGATGA
AATTAGAGTTTATTTAGGCTGCACTGGGTTTGCAGGGAAAATTAGTCTAGGCCTGGCACTGTGGCTAACG
ACTTTGGGAGGCTGAGGCAGGTGGATCACCTGAAGTCAGGAGTTCGAGACCAACCTGGCCAACATGGCAA
AACCCTAAAAATGCAAAAATTAGCTGGGCGTGGTGGCAGGTGCCTGTAATCCCAGCTACGCAGGAGGCTG
AGGCAGGAGAATCGCTTGAACCTGTGAGGCGGAGGTTGCAGTGAGTCGAGATTGCGTCATTGTACTCCAG
TCTGGGCAATAAGAGCGAAATTGTGTCTAAAAAAAAAAAACTAGTCCATAGGCAACATTGCCCTTAAATA
ACCACAAGAAAAAGGTGGATTCTTTCTTCTGGAAGAAAACCAAAACCCAAAACTATAGGTACTATTTTGT
GTGTCTTAGAAATGCGAACATCCTTCCTAAAGGTAAGAGAATGGATGAGGTGGTTTTGGAAGGCCTATCT
AGTTTTGCGATTCTATAAGCCAAACAAAGACAAGAGACGGCTTTGCTTTATGTTACAGCAGAAGCTAATT
GTCCACCCTTGTCCCTATGTCTTTTCCCTCTGCCTTCATTTACTGTTGACATCCAGTGAGGACTATCCTT
CTGACAGGGTCAGCTAATAATATGCAGGGTTAGTGAAAAATGAAAATGCAGGACCCCTTATTCAAACATT
TTTAAGAATTTCAAAATAGCACCAGTGGAGCACTAAACCAAGTGCAGGGCCCTTTCAAGAGCAGGCTCTG
TGTGACTACATAGGTTCATATCCACAAAGCCTGCCTACCTCCTAACCTCTTAGCTCTTGCCACAGCTCTC
CCTCAGATGATTTCTCGGGGAACACGTGGTCTGATAAACCTGTACTTTCTTGGAAATCAGGGACCTTGAG
GGGCCACACCATCCATCTCTGGCTCTGTCCCAGACATAGATTTGCTGTCGTGTCAGCCTCTGGGTGGAGA
GGTGTCATCTCAGCTTGTTTTCTTTACATAGGGACGTGCTGTAGAGAGCACAGCTGCTCATCTCAGTTGT
ACCCAAGATGTTTGGCAAGGCAAAAGTAAACTGCTTTTTGAAAAAGGAGGAAATCAAGGCATGGAGGAGT
TGACTCCATCATCCAGGAGGACTTAAACTAGCCCATGCAGACACTTGGAATCAAAGGGCTCTGGAACTCT
GTCCCCTTGGTGTTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTGTTGCCCAGGCTGGAGTGCAATGG
TGGGATCTCGGCTCACTGCAACCTCTGCCTCTTGAGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTA
GCTGGGACTACAGGCACATGCCACCACACCTGGCTGATTTTTGTATTTTTAGCAGAGATGGGGTTTGACT
ACTTTGGTCAGGTTGGTCTCAAACTCCTGACCTCAAGTGATCCACCCGCGTTGGCCTCCCAAAGTGCTAG
GATTACAGGCCTGAGCCATCACACCTGGCCTGTCCCCTTGATTTTGAGTCTGGGCTCTTTCTGCTTAACC
AACATGGCTTCTTGTACTTTGCTTATGTTTTTCTTCCCCTTTGTATAGTATCTGCCAAGTCTCTAGGGAG
TGTTCCCATTTCTACCCTCCAGAGTCAGCAGGGGTGGTCACCCCGTCACCCCGACCCCACAGGGCTAGAT
CACCAATAGCCCAGAATGGGCCACAACATAATACCCCCATGGACTCTGGCCACTTGATTCCCTTTTTCCT
GCCCCCACCTAACCACAGCCATCTCAGTCACTGCATGATGACAGGAGCTAGAGGGGTGCAGGCTGATGAC
AGTTAATTTTGTTGTTGCTTTCCTTGTTGACTGCACTTTGGTAGAAATGTGTTTTAACCAGATTATTGCA
AAGCAGTGAGTGTCGCTCTGGTTTAGGTATTTGCAGCCAATTATGGTTTAATGTGAATGACAACAGGCAG
ACAACAATCTGCCAAACAACTCTGGCTTGCCAAGCTCCACAGAGTCGGGATGTGCTCCCAGACCCAATGG
CTGGAGAGCCCCCACTCTTATCAGCAAGGTGATGTTTCATCTCCCCCAGCCAAGCTCTTCTCCCGGCACC
TCCTGCTCCATCACTGGCCTTTGCACTTCATCTCTGCCCTGGCTCATTTCTTTGTGACACTCTTCTCCAG
TGGGAGATGGGGATGAAGGAGAAGAACCCTGCACTCAACTCAGAGGTCTGGTCAGGTCAAGAGTTGTAGC
TATCTTGTCCCGGGAAAAAGCACTGGGCTTGGAATCGGAAGATGTGGGGGCACATCCCAGCTCTGTCTCT
TACTCAACTTACTCACTTAAGGGAGCTCAGCTTTCCTTTTGAAAATCTATAAAGTAGAGGCTATTACCTA
CCAAGTTTGCAGCGAGGATTAAATAAATCACACCATATGTGTGAAAGTGTCCGGCACACTATCTGACACA
TATTGAGCCGTTCATTGTTTGGTGAATCAGAGAAACGGGAGATTTCAAATGTCATTTTTGTCCAAATCCA
ACAGCAAAGGGTCCAGAATACTCATTTGTTTAATAAACATTTATTGAATACCCACTGTAAGATAGGTCTT
ATTTTTGGCCCCTTGGGGGATTATCTAACTACCCCAGAGATGGGATGAGATGACAGTCCAGGCCGTAGTG
GGGAAGCAGCCCTGCCATTCACCTGTTTCTCTTTAGGAAGGATGAGAATGGAGTGTGTGCTTATTAAAGA
CACTTGCCATCATCATTTCAAAGAAGTGAGGTATAAAAAAATGGCAACAATAGTAGTAATTTTATGAGAC
CCAAATTCCAAGCTACAAGAGCAGCTCTGTGTGGCCAGCACAGTGCAGGCCAGCAGGACAGCTTCAGGCG
GCCTCCACTGAGACGCACTCCTCCACCCCCTGCATCTGTGCTCCTGGAGACTGAGCTGTGGCTTGCAAAG
ATGGGACCACTGGTCATGCTTGACCAGTAGGGAGAGGAAGCAGGCAGGGGAAGCAGTGGCAGGAGATGGG
AGGAGGGAGGAGGGCCGAGACGGGGTACTTAGCTCTCCATTTCTGCCCCAGTGGAGTCAACACCAGGCAG
GCTAAGAGAAAACCACAGGTCCTATCTGGCAGCCTCTGCACAGCTTTCTCTTCAGGTCTGGTCACCACTC
CAGCCCTTGACTCCTTCAGGCCTAAGGGTGGTAACAGATCACCCAGTCCCCGAAAAGCCCAGGGGTCTGC
CCGCCATCTTGTGGTTTCCCTAAAGCCTGCTCACACCTTGCAGATAGTCTCATTTTTGAGCCGTCTTCAA
ACTTCCAAATTTAATTGTGCAAGTTTTCCTGCCCGGAATGATAGACACACAGGTGCTAAGGAGTTCAGAA
TCCAAGGAAGGAGATAGATTAGACCAAAAAGTAATACAATAAGCCAGGCACGGTGGCTCACACCTGTAAT
CCCAGCACTTTGGGAGGCTGAGGCAGGGGGATCACCTGAGGTCAGGAGTTGGAGACCAGCCTGGCCAACA
TGGTGAAACCCCATCTCTAGCAGAGCTTGCAGTGAGCCGAGGTCGCACCACTGCACTCCAGCCTGGGTGA
CAGAGCGAGACTCCGTCTCAAAAAAAAGAAACCCCGTCTCTACTAAAAATACAAAAAATAAGCCGGGCAT
GGTGGCACTCACCTGTAGTCCCAGCTACCTGAGAGGGTGAGGCAGGAGAATTGCTTGAGCCTGGGAGGTG
GAGGTTGAGCTGAGATCGCGCCATTGCACTCTAGCCTGGGCAACAAGAGCGAAACTCCGTCACACACACA
CACAAAAGTAATACAATATATTGCATAATAAGTGGTTTCAGAAGGAAGATTTTAGTTTCAAAACAGTTTC
TCACTTACCAGCTGGGTGAGTTTAAACAAGGAATTCAACCTCTTTGAAACTCAGTTTCCTGGTTTGTAAA
ATGAAGTTAATGATACCTGTTTGTAAAATGAAGTTGATACCCAGCCCTTAAAGTTGTGAAGACTGAGTAA
GTTAAGTTTATGAGCCACTGAGTCTGGTGCCTGGCACACAGCAAGTGGTAAGTAAATGCTAGCTGCTGCT
TCTTATTATCACTATTAATAATATTAATATTAGCTCTGTGTAAAACTTAAAGGATTTTTTTCTCTTTTCT
AAACCAAAAAGTAACCTGAAACATATTTCTAAGATAAATTAGACAGATTTTTCACATTATGCAAAAACGT
TGGGTCTTCGGGGAGAATTCCTGGCTATGAATTTGGCCAAATCTGAGTCTGTTGGCCTAGATCCTCTTTT
CATCAGACCACTTGTTCCCGGCCAGGTGGTGATGCGCAGGCACCCCCTGGTCTATGTCGTGAGTCTGCTG
ATTCCTAGCATCTTTCTCATGCTGGTGGACCTGGGGAGCTTCTACCTGCCACCCAACTGCCGAGCCAGGA
TTGTGTTCAAGACCAGTGTGCTGGTGGGCTACACCGTCTTCAGGGTCAACATGTCCAACCAGGTGCCACG
GAGTGTAGGGAGCACCCCTCTGATTGGTAAGCAGCCTCGGGGTCACTGGACACTCATCTTACATGACCCC
TGTGAAAGATCTAATGCTGGCCAGGCATGGTGGCTCATGCCCGTAATATCAGCACTTTGGGAGGCCAAGG
TGGGAGGACCACTTCAGCTCAGGAGTTCAAGACCAGCCTAGGCAACATAGTGAGACTTTACTAAAAATTT
TAAAAATCAACAGGGCATTCAGTGCATACTCACTACAACCTCTGCCTCCTGGGCTCAAGCAATTCTCCTG
CCTCAGCCTCCTGAGTAGCTGGGATTATAGGCACACACCACTACACCTGGCTAATTTGTTGTATTTTTAG
TAGAGACGGGGTTTCACGATGTTGATCAGGCTGGTCTTGAACTCCTGACCTCAGGTGGTCCGCCCACCTC
AGCTTCCCAGAGTGCTGGGATTACAGGCATGAGCCCACTGTGCCTGGCCGCTTTGTTTGTTTTTATCCCC
AATTTCAACCAATTACCCAAAGTGACTGAGCCATTGATTTCAGCTATTCAGGAAGCTGAGGCAGGAGGAT
TGCTTGAGTCTAAAAGTTTGAGTCTGCAGTGAGCTATGATCCCACCACTGCACTCCAGCCTGGGTGACAG
AGCAAGAACGGAAGGGAAGGGGAGGGGAGGGGAAGGGAGAGAAAGAGAGAAAAAGAAAGAGAGAAAGAGA
GAAAAGAAGGAAGGAAGGAAGGAAGGGAAAAAAACAGAAGAGCAAGCCAGATGCAGTGGCTCACACCTAT
AATCACAACATTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAGCCCAAGAGTTCAAGACAACATAGTGAG
GACTTGTCTCTCTCTCTTTTTTTTTTTTTTTTGAGATGGAGTCTTGCTGTGTCTCCAGGCTGGAGTGCAG
TGGTGTGATCTCCGCTCCCTGCAACCTCCGCCTCCTGGGTTCAAGCAATTCCCCTGCCTCAGCCTCCAGA
GTAGCTGGGACTACAGGCGCCCGCCACCATGCCTGGCTAATTTTTTGTATTTTAGTATAGACGGGGTTTC
ACTATGTTGGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCCACCCGCCTCAGCCTCCCAAAGTGTTG
GGATTACAGGCGTGAGCCACCAAGTCCGGCCGTGAGGCCTTGTCTCTACCAAAAATAAAAAAAAATTAGC
CTGGTGTGGTGGTGTGTGCCTGTAGTCCCAGCTATTCAGGAGGGTGAGGAGGGAGAATCACTTCAGCCCA
ATACGTTAAGGCTACAGTGAGCCATGATTGCACCACTGCACTCCAGCTTGGGCAATAGAGCAAGACCCTG
TCCCTAAAGAAAACAAAAAAATCTGATGCTGCATTTCAGACTGGAGGCTAACTGCACCTCTTCTGGCTTC
TCTCAGGGCACTTCTTCACCATCTGCATGGCCTTCTTGGTTCTCAGCTTAGCTAAGTCCATCGTGTTGGT
CAAATTCCTCCATGATGAGCAGCGTGGTGGACAGGAGCAGCCCTTCTTGTGCCTTCGAGGGGACACCGAT
GCTGACAGGCCTAGAGTGGAACCCAGGGCCCAACGTGCTGTGGTAACAGGTGTGTGAGAAGCCTTGTGTT
TCTCCCCCGTCTGGATTGATCACCTTAAAAATTCATTCCCGTTGATGATGTACTAGGTATTGCGTTGGCC
TATGTGAAAAACCTACAACAACTGCTTCTTAGCCTGGGTGTGGCATTTTATATTATCTCTCTGGAGACTG
AAGGCTGCCTGCTTCTGGCTTTCTAGAAAGTCTCATTTTGTTGTTTTTCTGTTTGTTTTGGTTTGGTTTT
TTGAGACAGGGTTTTGCCATGTTGCGCAGGCTGGTCTCGAACTCCTAGGCTCAGGCGATCTTCCCTCCTT
TGCCTCCCAAAGTGCTGAGATTGCAGGTGTGAGCCACTGCGCTCAGCTGAAAGTCTAATTTTGGCTTGAA
TTTCAGGCCCTCAGTTACTCTTTGTTTTGACAGAATCTCGCTCTGTCGCCCAGGCTTGACTGCAGTGGCA
CAATCTCTGCTCACTGCAGCCTCTGCCTCCCAGGCTCAAGCTATTCTCCTGCCTCAGCCTCCCGAGTAGC
TAGGACTATAGGCGTGTGCCACCATGCCTTGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCAT
GTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAGAGTGCTGGGA
TTACAGGTGTGAGCCTACCGCACCCAGCCTATTTGTTTGTTTTTATCCCCAATTTTAACCAGTTACCCAA
AGTGACTGAGCCATTGATTTCAGAATAGCACAGTGCTGGCATGCACGTGCACACATGCATACAAGGAGCT
TGTGTACATTTAGGTTCTAGTTGCTTAATGTGAACAAGTATAAGAATCATCCCAGCCCCTAAAGCTCCTA
CTGAGTCCATTTGGGCTGACCTCTAACTCAGAAACTCTCCTGGACGGATCTAGCACACATACACCCTGCT
TACATAATAACAGCTCTGACGTGTTCTCTGAGGCTTCCTGCAGTACAACTTTGCCTCGCCTAATCGGGTG
GGGAGAGTACCCAACCCAAACACAACCCACTGCTCAGTTTCACCTGCAACTTTCCTTGAAGGATGAGGCC
ATCAGCTTCAAGAGAAAGTCCAGGTGGGTCTTGGTAGTCCCTGGCTCACCCAGGGTGTTTTCCTCCATCA
CACAGAGTCCTCGCTGTATGGAGAGCACCTGGCCCAGCCAGGAACCCTGAAGGAAGTCTGGTCGCAGCTT
CAATCTATCAGCAACTACCTCCAAACTCAGGACCAGACAGACCAACAGGAGGCAGAGTGGCTGGTCCTCC
TGTCCCGCTTTGACCGACTGCTCTTCCAAAGCTACCTTTTCATGCTGGGGATCTACACCATCACTCTGTG
CTCCCTCTGGGCACTGTGGGGCGGCGTGTGAAGACTGAAGTGTTCTTCAGTAATTGTGCTGGCACTTAGG
AGAGAGAGGAGGGGGAATAATAGTGGGTTAAAAAGCTTTCTGGGTCGGGTGTGGTGGTTCTTGCCTATAG
TCCCAGTGCTTTGGGAGGCCATAGCAGGAGGATTGCTTGAGCCCAGGAGTTCGAGACCAGCCAGAGCAAC
ATAGTGAGACCACATCTCTACCAGTAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAGCTG
GGCATAGTGGCTCATGCCTGTACTCTCAGCTACTTGGGAGGTTGAGGTGGGAGGATTGCTTGAGCCCAGG
ATTTCAAGGCTGCAGTGAGCCATGATTGCACCACTGCACCCCAGCCTGGGTGACAGAGCAAGACCCTGTC
TCAAAAAAAATAAAATAAAAGGCTTTCTGCCTTCATTGCCTCTCCATTGAGTCCAGCTTCATACACCTAA
TAGTCTGCTGTGTGACCCAAGGATCATATGTTCATGAAAATGTATAAACAGTATGAGTATACAGTAAAAA
AGTAAACTTTCCTTCTTATCCCAGTCCACTCACCCAAAGCAACCACTGTTAGTAATTTCTTCCAGAAGAT
TTCTAGGCACACACATGCATCTATGTATGGTTAAGCAGTTTTTTAAAAAGTGTAAGATAGCTCCATCTTC
CAGCCCTGCCTTGGCCATTTATTCAGTCACCCATTCAACAAAACCTTTATTGAGTATTAAGTCCTAGGCA
TTATGTTAGGCACACCAGAGAATACAAAATAAGTCAGCACAGGTTATTATTCACTTGTTGTGATTCCCAT
GGTCAACCTGGTACCAACCAACCAGAGCATAAAATTCCTGCCATATCCCTCACCTTCTGAGGCTCTTGTT
ACCACTTCCCAGCTAAGTCCACTGCCTCCACCCTCTTCCTGTGCCCCTTCAGCAGTGCATCCCCACTGGA
GAGAACCCAGGTGCCAAGGCTTCTTTTTTTTTTTTTGAGGCAGAGTCTTGCTCTGTCACCAGATTGGAGT
GCAGTGGTGCAATCTTGGCTCACTGTAACCTCCACCTCCCGGGTTCAAGCAATTCCCCTGCCTCAGCCTC
CCGAGTAGCTGGAACTACAGGTGCATGCCACCACACCCAGCTAATTTTCTGTATTTTAGTAGCAGGTGCC
AAGGCTTCTAAGCCCAGTCTAGCACATCATTTAGTCCACACACAAGGGGCAATGCATGGAGTGGGAGGAC
AGGCATAGTTCACGGTCCTTTGCTTCAAGGAAGTTATAATCCACTGTCCCTGAACTAGTCAGCTAGGAAA
TACTACACACTAAGTTGCTTTAAGAGAGATTTATTTTCTCACCAGTCTGGAAGCTAGAAGCCTAAGATCA
AGGTGCAAACAGGGTGGGTGGCTTTCTTCTGAGCCCTGTCCTTGGCTTGCAGGTTGCTGACTTCTTGCTT
TGTTTTCACATGACCTTTCCTCTGTGCATGCTCATGTCTGTGTCCAGATTTTCTCTTCTAACAAGGGCAC
CAGTGTTGTTGGTTTAGGGTCCATCCTAGTGACCTCTTTTAACCTTAATCACTTTTTTCAAGGCCCTATG
TTCAAACAGTCATATTCTGAGTACTAGGGGTTAGGATGTCAACATATGAATTTTGGGGAGTGGGCAGCAG
ACAAAGGGGACATATTTCAGCCCATAACACTAGTGATTCCGGAGGTTTAGCAAGTCCAGATTCTGCAGAG
TAGGCTGGCAGGCTGGAGACCTGCTGACAGTCTTAACGCTGTCAGTAGAAGACACAAAAGTCCAAAAGCT
GTTTGCTAGAAACTTTTTCCAGCACTAAAATTGCCTTTAGAAAAATCTGCCCCCCTCCATCCCCCCAAAG
AAAAAGAATTTAAATTCAAACAGCCTTTACCTGCATCTTCCCTCTCTCATTACCTCCCAGTTAGTGCTCA
GCGCCAATTGTCCATGAAATTCCACTGCTGCTGTTAATGAAATGAGGAACATAAGAAGTTTGTGAACAGG
AAGTGTCCATGCAAACCTCCACCTGTTTCCTTCCTCACTTCCTACTTTCTACTGCTGGCCTCCTCTGCTT
TGACGTGCTCAGTTAGGCATCACGGTCCTGATGTCACCTTAGCTTGCCATCCATGTCGATTCTGGCTTTC
TCCACTTCCTCCTCCAGTTAACAGAGGAGGAAAGGAGGAAAAGGCCTGAGGTGAACTCCTCACTGTGGGG
TCTCTCTCCTTCCCAACTATGTCTTAGAGAAAGCACCAGTGTCAGAGTCAGGAGTCCTGATGATTACGGT
CCTGGCTCTGCCATTACCTAGCCAGGGGACCTCTGTGAGCTTAACTTCTCAGACTTCAACTTACCTTATT
TGAAATTGGGGCTGTTTATATTTGCTCTACCCCTATCAAAGAGTTATGAGGATCAAAATGAAAAAATGTA
TTGAACAATGCTTTGAAAAGTAAATAATGGGGCGTGGTGGCTCACGCCTATAATCCCAACACTTTGGGAG
GCTGAGGTGGGTGGATTACCTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCCTGTCT
CTACTAAAAATACAAAATTAGCCAGGCGTGGTGGCACATGCCTGTAATCCCACCTACTCGGGGGGCTGAG
GCAGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCGGTGAGCCGAGATCGTGCCATTGCACTCCAGCC
CGGGCAACAAGAGCTAAACTCTATCTCAAAAAAAAAAAAAAGTTTAAAGTGCTTTGGGAAGGGGGAGGGA
TAGCATTAGAAGATATACCTAATGTTAATGGGTGCAGCACAGCAACGTGGCACGTGTATACATATGTAAC
AAACCTGCACGTTGTGCACATGTACCCTAAAACTTAAAGTATAATTTAAAAAAAAGTAAATAATGCATCA
CAAAATAAAGATAAGACCTGCTTGCTATCAACAGATGGACTCCAGTCTTGAGTTTTTGGTGTGGGATCTG
ATTTCCAGCTCCCTCTATCAGAATCCAGTCATGGGGTCTGGTACTCTCCTCCAGTTGTTCCACTATTATA
GGATCCAAAGGCCAAGGGCTGAAGTCATCACATGCCTCCTTACAGAGAAACTTGTGTGGCAGGGGTTGCC
AAAGGGACTTCCCAGTCCATCTCACATGAAGCTCACCTTTGTTCCATTCGCAGATGACATTGCCCAGGTT
CTTTACATCAGCCTCTAACAAGCAGCCCTTTAAGAGTGAAGCCTGGCCGCCAAACTGGTCCCCGGTACAC
TTCCTTCGTCTAGTGGCAAGTGGCCTTAATCACAACTGCAGCCCAGAACTCATTCAAAGCCCCAGAGTCA
GGCCTGCATCCTGAAGCAATGACATTGTAAACCAAAAATAAAATTCTAAGGGCCCCCCAACCATCTGAAT
GGACTCCATCCTTTCAGTGAGGAC

# data[].attributes.headline
기억유전자 관련 논문2

# data[].attributes.subject
기억유전자 관련 논문2

# data[].attributes.body
(1) GeneBank accession : GR956068.1
number:TTACGGCCGGGGATTCATGTACATGCAACTGTGGCGCACCATGACAGGTCACCTCTCGAGAAACGCCTGA
TCCTCGTGATCCTCGCCTTCATCATCTGCCTCCCGGAATTCTTCACCCTGTACGGAGTATCCGAAGTTAT
CCCCGTCTGTCAGCCTCATCGACGTTGTGAAGCAGGGGATCAAACGAGAAAAAGGGAGGATGAAAAGGCT
GGGGATGCAAAGGTTAAGCGAAAACTCGTGTGTGACTCCTCTCACACGCCTGGCAGTGAACGGTGGGAAC
AGGCCTGCATCCCCAGGAATCTGGGCTACCTGACAAACGCTGCTTCAGATTCCAGAGGTGGCAGCGAAAA
TCCTGTCATGAACTTTTTCATGTGTGAAACTGATCTCAACACTTCAGGATTACTCGGCCACGTAGCCGCT
CTCCAGGAACATGTGGAAGTCTCTCTGCGATTTCTACTCAGACTACGTATTTCATTTAATCTCACCTTGG
ATGACCGGACTAACTGAGCAACCTGTACCTTGTACCACCTGAAGAGAGGATGAAGAGGAGAAAGGGGAGG
AGGATGCTTTCTACTGCTGTCTTCCTGTCCCACCCACTTCAGAGACAGCCAACCACAGCCGATGCCTCCT
TTGGCTTGCCAATCAAACTGTTTTGGTTGCAACGGGAAAGGAAAAGCTGTCATGGAAACCGACACAAAAA
GACGAGTGGCGGTGTGCGTTCAGGTGCTCTGGCTGGTTCTGCTTTGTGTTGTCCTCCTGGCTGTAGCCAC
CATTGTGCTGAAACGCATCTACTGCAAACCCAGATGCAACTGTGAGTACACTTTGCTGCACAGCTTCTAA
TGTTGGGAAGAAGCGTGCTGAGATCAATAACACTGCCAAGGGA

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.headline
1-2 기억 유전자 논문-구글 스칼라

# data[].attributes.subject
1-2 기억 유전자 논문-구글 스칼라

# data[].attributes.attachment_link.description
It has been known for several decades that the formation of long-term memory requires gene expression. In recent years, the use of genetic and molecular approaches has led to the identification and characterization of genes and molecules that play a fundamental role in the biological mechanisms underlying learning and memory.

# data[].attributes.body
(구글 스칼라로 검색한 결과)

# data[].attributes.headline
기억 유전자 관련 논문 

# data[].attributes.subject
기억 유전자 관련 논문 

# data[].attributes.body
GeneBank accession number: AY892288.1
ATGAGTGAAGAGGAGCAAGGCTCCGGCACTACCACGGGCTGCGGGCTGCCTAGTATAGAGCAAATGCTGG
CCGCCAACCCAGGCAAGACCCCGATCAGCCTTCTGCAGGAGTATGGGACCAGAATAGGGAAGACGCCTGT
GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCACCAGCCTAATTTCACCTTCCGGGTCACCGTTGGCGAC
ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC
ACCTCAAAGGGGGGAGCATGCTGGAGCCGGCCCTGGAGGACAGCAGTTCTTTTTCTCCCCTAGACTCTTC
ACTGCCTGAGGACATTCCGGTTTTTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTGTAGTCCTAACC
AGGAGCCCCCCCATGGAACTGCAGCCCCCTGTCTCCCCTCAGCAGTCTGAGTGCAACCCCGTTGGTGCTC
TGCAGGAGCTGGTGGTGCAGAAAGGCTGGCGGTTGCCGGAGTACACAGTGACCCAGGAGTCTGGGCCAGC
CCACCGCAAAGAATTCACCATGACCTGTCGAGTGGAGCGTTTCATTGAGATTGGGAGTGGCACTTCCAAA
AAATTGGCAAAGCGGAATGCGGCGGCCAAAATGCTGCTTCGAGTGCACACGGTGCCTCTGGATGCCCGGG
ATGGCAATGAGGTGGAGCCTGATGATGACCACTTCTCCATTGGTGTGGGCTCCCGCCTGGATGGTCTTCG
AAACCGGGGCCCAGGTTGCACCTGGGATTCTCTACGAAATTCAGTAGGAGAGAAGATCCTGTCCCTCCGC
AGTTGCTCCCTGGGCTCCCTGGGTGCCCTGGGCCCTGCCTGCTGCCGTGTCCTCAGTGAGCTCTCTGAGG
AGCAGGCCTTTCACGTCAGCTACCTGGATATTGAGGAGCTGAGCCTGAGTGGACTCTGCCAGTGCCTGGT
GGAACTGTCCACCCAGCCGGCCACTGTGTGTCATGGCTCTGCAACCACCAGGGAGGCAGCCCGTGGTGAG
GCTGCCCGCCGTGCCCTGCAGTACCTCAAGATCATGGCAGGCAGCAAGTTG

# data[].attributes.headline
기억 유전자 논문 - 구글 스칼라

# data[].attributes.subject
기억 유전자 논문 - 구글 스칼라

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
Genbank accession number : AY211956.1
FASTA

TGAATGTAGAAAAGGCTGAACTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAAAAAACCAACAGAGCAG
TCTGGATGAAAGTAAGGAAATATGTAGTGCTGGAAAGACCCTGGGTGCCCATGAGCTGAATGCCCATCAT
CCATGCGAGAGGAAAGAACTAGAGGATGAGCCACAGCACCCTGAGAGCCCCAGAGGTAATCCTCAGAACT
GCCAGTCTGGAACCAAACTGAAAAGTAGTATTCAGAAAGTTAATGAGTGGTTATCCAGGAGTAATGATAT
TTTAACTTCTGATAACTCCTATAGTAGGAACCATGAGCAGAATGCAGAGATGCCTAGTGCCTTAAAAGAT
GGGTATCCAGATACTGCAGATGCAAATTCTAACATTTCTGAGAAGACTGACCCAGTGGCTGACATCACTT
ATGATCCCTGGCCACATGTGCCTGAAAGAAGCTGCCCCAGGCCAGCAGAAAACAATAACATTGAAGATAA
AATATTTGGAAAAACCTATCGGAGAAAATCAGGTCACCCTCATTTGAATTGTATAACTGAAAACTTGTTT
GCTGGAGCTGTTGTTGCTCCTGATTCTTTGATCCCTCCAGAGCCTCCAAAAACAACCAAGTTAAAGCG

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
GenBank: OM773474.1

CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCAC
TCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGG
CTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAA
GGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTAC
AGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACA
TCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTAT
GTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAAC
TCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACC
AGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCC
GATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGA
ACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTA
TGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCT
GGTAAAGCTTCATGCACTTTGTCTGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCC
GTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTT
TGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAAT
TCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGAT
CTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCA
TTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAAT
TTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAG
CATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAAC
CATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAAC
AAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAG
GTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGG
TGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTG
GAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTA
CAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGC
ATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCT
GTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTG
ATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGT
TGTTCAGTTGACTTCGCAGTGGCTAACTAATATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTT
GATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTA
TCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGT
TCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCT
AAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTA
AATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGA
AACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAA
CCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCG
AAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACT
CAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGT
GTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAG
TTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACC
AGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTT
GATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAG
AAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTA
CCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGAT
TGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTATTA
TTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGT
GAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAA
GCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTG
CAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGG
ACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTC
GGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACG
AAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTTGTGCTGACCCTATACATTCTTTAAGAGTTTG
TGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCA
AGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTA
AGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTG
TGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGAC
ATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAG
ATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAA
GGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACT
TACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTG
CCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTT
GCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATA
GTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT
TTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCT
TGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT
CTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTT
CTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTC
CTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACT
AGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTT
TGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGA
CATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAA
CCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTT
TTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAA
GTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACT
GCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAG
CAAGGGCTGGTGAAGCTGATAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTT
AGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTG
AACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGG
GCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAA
ATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCAT
GGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTA
AAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGA
TGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTT
TGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTTACAGAGCAACCAA
TTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATAT
CAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACA
TTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAG
GAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACC
AAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTG
AAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAG
TGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACAT
TATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCT
TATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCC
TTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTT
TCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTAT
ATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAG
CATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATT
TAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTACAATTTGGTTTTTACTATTAAGTGTT
TGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTT
ACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTC
TATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAA
ATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATA
TTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGC
AGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCA
GCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAG
ACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAAC
TATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACAC
AATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAG
ACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTAC
AGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTC
TCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAG
TTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTAT
GTGTCAACCTATACTGTTACTAGACCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTT
AAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACAC
TAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTC
AGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCA
CATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAA
ACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAA
AAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAA
ATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTA
ATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAA
AGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAA
CATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCAT
CTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTA
TACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGT
TTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAG
TTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTT
GGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTA
CTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTA
TTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAG
GCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAAT
GATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTA
CACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTAT
CGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTT
GCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTAC
CTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACA
TATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATT
TCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCT
TTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAG
TGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGT
GGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCA
GTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGG
TTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACT
ACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTCCAAGACATGTGATCTGCACCTCTGAAGACA
TGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAA
TGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAAT
CCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACA
ATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAA
TGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATG
GAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGC
AAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGT
TATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATG
AAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAA
TTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATT
GGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTC
CAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTT
TAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGC
TATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTG
TTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGC
GTATTATGACATGGTTGGATATGGTTGATACTAGTTTGAAGCTAAAAGACTGTGTTATGTATGCATCAGC
TGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTT
ATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGG
CTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTAT
TGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTA
GTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTA
GACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACGGGGACT
ATTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCT
TGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAG
TTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACAT
TCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATG
CAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAG
CCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGT
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTT
GACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAAC
AGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAG
AAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATA
ATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTG
ATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAAT
TGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTA
AGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTG
CTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGG
TAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGA
ACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAG
TGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGC
CACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCT
TTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTG
TTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCA
AGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGA
TTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACAC
TTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGA
ACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTA
CACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGC
TGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATT
GATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTAC
TTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACA
TATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAA
GGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAA
AGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACG
CCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACA
TTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCTAGGTAGTG
GAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGC
AGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACG
GAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTA
ACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACT
TACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATAC
CACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGG
AATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCAC
TACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCTGGTAATTTTAAC
AAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACT
TCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAAT
GTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGC
TGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGG
GTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACG
TAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACC
GTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAG
CCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAA
AACTGTTTATAGTGATGTAGAAAACCCTCATCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATG
CCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCAC
ACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACT
ATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATT
TGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATG
TCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA
TGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTC
AATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAA
ACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTG
CTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGA
ATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCG
TGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTT
GTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATG
CTTACTAATGATAACACCTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAG
TCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACG
TAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCT
GTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTA
TGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGG
TTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGG
ACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGC
TCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAG
AGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGT
TATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATG
CTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATAC
AGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCA
ACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATT
CTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTC
TGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATAT
TTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAG
TGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGT
TGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAG
CACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAG
AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTG
TCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACAT
AAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAA
TTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTT
TATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGAT
TCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATG
TAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCT
TTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAAT
GTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACC
TCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGAC
CTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTT
ATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTA
CTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGT
ACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGA
GATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTG
TACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTT
TGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGT
GCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCT
ATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTA
TTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCAC
GAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATG
CGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCA
CGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCA
CAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAAT
TCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAG
ATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAA
CATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATT
ACTCTGACAGTTCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTC
TGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTG
TATCNNNNNNNNNNNNNNNNNNNNNNNNCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTT
ATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGG
ACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGAT
GGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTA
AGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATAC
TGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGAC
ATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTC
AAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACC
ATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTC
AATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATT
TACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGA
ACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGT
GGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTC
CTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTC
TGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAG
GTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAA
CATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAAT
GCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATA
ATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATA
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACA
GTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACT
TTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACC
CTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTAT
ACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTT
TATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAG
CATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAA
TTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTT
CCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTC
TTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAA
CAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTAC
AACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT
TTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCC
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGG
TGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCG
AAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTT
GTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT
TTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAA
CAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTA
AGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTT
GCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGT
GATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTC
TATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAAC
AAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCA
ACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCA
GATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATA
TAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTT
ACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTG
GAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAA
CAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAA
CCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTT
TTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAG
AGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAAT
TTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTA
ACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGATGACACTACTGATGCTGTCCGTGATCC
ACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACA
AATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATG
CAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTG
TTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCT
AGTTATCAGACTCAGACTAATTCTCATCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACA
CTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCATAAATTTTAC
TATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATT
TGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTG
CTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTA
CAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCA
AGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAAC
AATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTAC
TGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATC
ACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGT
TTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAG
TGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTC
AACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTG
TTTTAAATGATATCCTTGCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGG
CAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCT
AATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGG
GCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCC
TGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAA
GGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTA
CTACACACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGA
TCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCA
GATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCC
TCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTA
TATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATT
ATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAAT
TTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGAT
TTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTT
CAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGG
CGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCA
CTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGC
TCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAA
CTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGAT
GCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTT
CAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTA
TACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTAC
CAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAA
TTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGT
AATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTAC
GAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTG
CTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAA
TATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCT
AGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTT
AGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTA
GTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTT
TGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGC
TGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGG
CTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAA
CTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGT
AATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAG
GACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGC
GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGA
CCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGA
CTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTT
GATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAG
ATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACT
CGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGC
TCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCT
TTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGT
TTTACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG
GCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGA
CTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCA
CTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAA
TCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTTAACATCAACCATATGT
AGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCA
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATT
ATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTG
TTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAA
CTAAATGTCTCTAAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGAT
TCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTAC
CCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGG
ACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA
CGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTG
GGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTT
GAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCT
CAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTT
CCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAG
AATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGC
AAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTA
AGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCC
AGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCG
CAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACAC
CTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCA
AGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAA
AAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTC
CTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGC
CTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATAT
AGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCA
CATAGCAATCTTTAATCAGTGTGTAACATTAGNNNNNNNNNNNNNNNNNNNNNNCATTTTCACCGAGGCC
ACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGT
GTAAAATTAATTTTAGTAGTGCTATCCCCATGTG

# data[].attributes.attachment_link.readable_text
1: Tonegawa S, Pignatelli M, Roy DS, Ryan TJ. Memory engram storage and
retrieval. Curr Opin Neurobiol. 2015 Dec;35:101-9. doi:
10.1016/j.conb.2015.07.009. Epub 2015 Aug 14. PMID: 26280931.

# data[].attributes.headline
기억 유전자 관련 논문_구글 스칼라

# data[].attributes.subject
기억 유전자 관련 논문_구글 스칼라

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.description
논문, 학술저널 검색 플랫폼 서비스

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
dbpia

# data[].attributes.attachment_link.title
기억및기억연구개념역사그리고과제

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE06376214

# data[].attributes.attachment_link.description
논문, 학술저널 검색 플랫폼 서비스

# data[].attributes.headline
1. 기억유전자 논문 - 구글 스칼라

# data[].attributes.subject
1. 기억유전자 논문 - 구글 스칼라

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.body
DRD4(homosapiens's dopamine receptor)
genebank accession number :
NM_000797.4

# data[].attributes.body
관심있는 유전자의 염기서열 검색
→  GenBank accession number :  찾아서 쓰기
→ FASTA : 염기서열 텍스트 복사해서 업로드

# data[].attributes.body
염기서열:
MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGD
NTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEV
CSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQ
PPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI
SPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFP
SPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNP
IQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVP
VAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPG
AGLGVGQGPWPMHPAGMQ

길이 498

# data[].attributes.body
단백질 이름: Interferon regulatory factor 5: 대식세포

기능: 환경적 신호들에 효과적으로 반응하여 사이토카인들과 미생물 신호들에 대한 반응으로 그들의 표현형과 기능을 변화시킴

구조: IDK
FASTA 텍스트 서열:

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGD
NTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEV
CSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQ
PPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI
SPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFP
SPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNP
IQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVP
VAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPG
AGLGVGQGPWPMHPAGMQ

- 길이: 498
- 질량: 56044
- 이성질체 개수: 6

# data[].attributes.attachment_link.title
초등학생의 비만유발유전자 다형에 따른 신체구성, 뇌신경세포 성장인자와 기억력과의 관계

# data[].attributes.attachment_link.readable_text
1: Tonegawa S, Pignatelli M, Roy DS, Ryan TJ. Memory engram storage and
retrieval. Curr Opin Neurobiol. 2015 Dec;35:101-9. doi:
10.1016/j.conb.2015.07.009. Epub 2015 Aug 14. PMID: 26280931.

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.readable_text
1: Guan JS, Haggarty SJ, Giacometti E, Dannenberg JH, Joseph N, Gao J, Nieland
TJ, Zhou Y, Wang X, Mazitschek R, Bradner JE, DePinho RA, Jaenisch R, Tsai LH.
HDAC2 negatively regulates memory formation and synaptic plasticity. Nature.
2009 May 7;459(7243):55-60. doi: 10.1038/nature07925. PMID: 19424149; PMCID:
PMC3498958.

# data[].attributes.attachment_link.title
초등학생의 비만유발유전자 다형에 따른 신체구성, 뇌신경세포 성장인자와 기억력과의 관계

# data[].attributes.body
단백질 이름: Interferon regulatory factor 5
기능 : TLR7, TLR8 및 TLR9 내분비종 톨 유사 수용체 하류에서 I형 인터페론(IFN) IFNA 및 INFB 및 염증성 사이토카인의 발현을 활성화하여 선천성 면역에 중요한 역할을 하는 전사 인자
FASTA 텍스트 서열:

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGD
NTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEV
CSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQ
PPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI
SPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFP
SPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNP
IQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVP
VAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPG
AGLGVGQGPWPMHPAGMQ

Length:498

Mass (Da):56,044

# data[].attributes.attachment_link.title
Creating a false memory in the hippocampus - PubMed

# data[].attributes.attachment_link.description
Memories can be unreliable. We created a false memory in mice by optogenetically manipulating memory engram-bearing cells in the hippocampus. Dentate gyrus (DG) or CA1 neurons activated by exposure to a particular context were labeled with channelrhodopsin-2. These neurons were later optically react…

# data[].attributes.attachment_link.title
Creating a false memory in the hippocampus - PubMed

# data[].attributes.attachment_link.description
Memories can be unreliable. We created a false memory in mice by optogenetically manipulating memory engram-bearing cells in the hippocampus. Dentate gyrus (DG) or CA1 neurons activated by exposure to a particular context were labeled with channelrhodopsin-2. These neurons were later optically react…

# data[].attributes.headline
기억 유전자 논문-구글스칼라

# data[].attributes.subject
기억 유전자 논문-구글스칼라

# data[].attributes.attachment_link.readable_text
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구 : Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice

Abstract

Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines. Particularly, embryonic stem-cell gene-targeting method allowed researchers to generate model mice having a gene knockout or mutation in a gene of interest. Furthermore, this approach enabled us to evaluate the relationships among different phenomena observed at molecular, cellular, and systems levels. On the other hand, since many pioneering neuroscientists such as Cajal and Hebb proposed the possible principle of information storage in the nervous system, lots of efforts have been made to search for the molecular and genetic basis for learning and memory in neuroscience.
In this context, the genetic approaches were early introduced into neuroscience field and contributed heavily to the advancement of our understanding for molecular mechanisms of learning and memory. Especially, transgenetic mice model have greatly expanded the knowledge about how the events in molecular and cellular level could induce the alteration of neuronal circuit, subsequently leading to the behavioral output. Here, using the two gene deletion (knockout) mice lines, I investigated the unknown role of two enzymes, PI3Kγ and CD38 in the brain. In particular, the physiological functions of these two proteins are evaluated utilizing both electrophysiological and behavioral approaches.
Phosphatidylinositol 3-kinase (PI3K) has been reported to be involved in key cellular processes such as cell survival, proliferation, migration, and adhesion. In addition, a growing body of evidence has revealed the role of this kinase as a capital player in synaptic plasticity and neural functions in brain. Nevertheless, the origin of functional diversity of PI3K in brain is elusive and less understood. In this study, I investigated the role of PI3Kγ, the only member of class IB PI3K, in hippocampal-dependent synaptic plasticity and cognitive functions. I found that PI3Kγ existed in hippocampus and played a crucial role in N-methyl-D-aspartate (NMDA) receptor-dependent long-term depression (LTD) at Schaffer Collateral-Cornu Ammonis (SC-CA1) synapses. Both genetic deletion and pharmacological inhibition of PI3Kγ disrupted NMDAR-LTD, while leaving other forms of synaptic plasticity intact. Accompanying this physiological deficit, the activation of p38 mitogen-activated protein kinase (MAPK) was impaired in parallel in PI3Kγ KO mice. Furthermore, the impairment of NMDAR-LTD by PI3Kγ blockade was specifically correlated with deficits in behavioral flexibility. These findings suggest that a specific PI3K isoform, PI3Kγ, is critical for NMDAR-LTD as well as behavioral flexibility.
CD38 is a multifunctional enzyme, mediating the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD to ADP-ribose. It has been shown that CD38 is implicated in a wide range of cellular processes such as cell cycle regulation, gene expression, insulin secretion, and transmitter release via Ca2+ signaling pathway. In addition, a recent paper has revealed the interesting role of CD38 as a molecule important for social behavior and maternal nurturing. However, the role of CD38 in hippocampal-dependent synaptic plasticity and behavior remains largely unknown. In this study, I found that adult CD38 KO mice exhibited the deficit in L-LTP maintenance at hippocampal SC-CA1 synapses. Moreover, this physiological deficit was accompanied by the impairment in novel object recognition task. These results demonstrate that CD38 is a key enzyme for L-LTP maintenance at hippocampal SC-CA1 synapses, thereby mediating specify hippocampal-dependent memory formation.
In summary, I found that the both class IB PI3Kγ and CD38 mediated the critical function in hippocampal synaptic plasticity and cognitive behaviors such as behavioral flexibility and memory formation. These data strongly propose that the transgenetic mice model is a powerful tool to dissect out the physiological functions of specific genes at molecular, cellular, and systems levels.
신경생물학자들의 연구 목표는 신경세포 수준의 변화와 동물 행동 변화 사이의 직접적인 상관관계를 밝혀내는 것이며, 더 나아가 이들 사이의 인과관계를 이끌어 내어 동물들의 다양한 행동들을 만들어 내는 분자, 세포학적 기전을 규명하는 것이다. 유전학적인 방법들은 이러한 목표를 달성하는데 지금껏 큰 기여를 하였으며, 특히 다양한 유전자 조작 생쥐 모델들을 대상으로 시냅스 가소성이나 학습과 기억의 바탕이 되는 분자 메커니즘이 연구되어 왔다.
유전자 조작 생쥐 모델을 이용한 대표적인 연구 성과로는 NMDA 수용체가 해마 의존적인 시냅스 가소성과 학습, 그리고 기억과 같은 고등 인지기능에 핵심적인 역할을 하며, NMDA 수용체를 통한 활성 신호는 인산화 효소 αCaMKII로 연결되어 해마의 생리학적 기능들을 조절한다는 것이다. 또한, 또 다른 인산화 효소 PKA도 NMDA 수용체 등을 통해 활성화되어 장기 시냅스 가소성 및 장기 기억의 형성에 중요한 역할을 할 가능성이 제기되었다.
앞선 연구들을 통해 여러 유전자들이 해마의 시냅스 가소성과 학습, 그리고 기억 현상에 중요한 역할을 한다는 사실들이 알려졌지만, 여전히 많은 유전자들의 뇌에서의 기능은 알려져 있지 않다. 또한, 시냅스 가소성 및 고등 인지기능에 관여하는 분자, 세포학적 메커니즘의 이해를 위해서는 이렇게 아직 까지 연구되어 있지 않은 유전자들의 구체적인 기능 연구와 이들이 다양한 상황에서 서로간에 어떻게 상호작용 하는지에 대한 통합적 접근이 필요하다.
이러한 맥락에서, 본 연구에서는 해마에서의 기능 연구가 전무한 PI3K class IB isoform, PI3K 감마, 그리고 이와 유사하게 다기능 효소인 CD38을 대상으로 해마 의존적인 시냅스 가소성과 인지 기능에 미치는 영향에 대한 실험들을 진행하였다. PI3K가 뇌에서 가지는 기능들의 경우 다양한 isoform의 특이적 기능들에 초점을 맞춘 연구는 전무하였는데, 본 연구를 통해서 신경세포와 심근세포, 조혈모세포, 그리고 면역세포 일부에만 발현되는 것으로 알려진 class IB PI3K 감마가 해마 시냅스에서의 장기 시냅스 저하 현상에 특이적으로 관여하다는 사실을 밝혀내었다. 또한, 이러한 해마 신경회로에서의 생리학적 변화는 동물 행동 유연성의 변화를 이끌어 내는 것으로 나타났다. 또한, 해마에서의 기능 연구가 전무한 CD38 유전자의 경우, SC-CA1 시냅스에서의 장기 시냅스 강화와 해마 의존적 장기 기억 형성에 중요한 역할을 할 가능성을 확인하였다.
종합하면, 이러한 연구들을 통해서 PI3K 감마와 CD38이 해마 의존적인 시냅스 가소성과 동물 인지행동에 중요한 역할을 할 가능성을 확인하였다. 또한, 신경과학 분야에서 그 기능이 연구되지 않은 많은 유전자들이 다양한 종류의 신경 생리 및 동물 행동에 다양한 방식으로 관여할 가능성을 시사하고 있다.

URI

https://hdl.handle.net/10371/156396

http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000000618

Files in This Item: There are no files associated with this item.

Appears in Collections:

College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Theses (Ph.D. / Sc.D._생명과학부)

# data[].attributes.attachment_link.title
SNU Open Repository and Archive: PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구

# data[].attributes.attachment_link.description
PI3Kγ 및 CD38 유전자 형질 변환 생쥐 해마에서의 시냅스 가소성, 학습과 기억에 관한 연구 : Studies on hippocampal synaptic plasticity,learning, and memory in PI3Kγ and CD38 knockout mice Abstract Transgenetic technology has been a dominant tool to study the complex cellular processes in various biological disciplines.

# data[].attributes.body
길이:498
이성질체 개수:6개
질량:56,044

# data[].attributes.body
(PubMed로 검색한 결과)

# data[].attributes.attachment_link.readable_text
Affiliations

¹ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA. marcoa@mit.edu.

² Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. marcoa@mit.edu.

³ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁴ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁵ Division of Newborn Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.

⁶ Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁷ Broad Institute of Harvard and MIT, Cambridge, MA, USA.

⁸ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA. lhtsai@mit.edu.

⁹ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. lhtsai@mit.edu.

¹⁰ Broad Institute of Harvard and MIT, Cambridge, MA, USA. lhtsai@mit.edu.

Free PMC article

Free PMC article

Affiliations

¹ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA. marcoa@mit.edu.

² Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. marcoa@mit.edu.

³ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁴ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁵ Division of Newborn Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.

⁶ Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA.

⁷ Broad Institute of Harvard and MIT, Cambridge, MA, USA.

⁸ Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA. lhtsai@mit.edu.

⁹ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. lhtsai@mit.edu.

¹⁰ Broad Institute of Harvard and MIT, Cambridge, MA, USA. lhtsai@mit.edu.

Abstract

The epigenome and three-dimensional (3D) genomic architecture are emerging as key factors in the dynamic regulation of different transcriptional programs required for neuronal functions. In this study, we used an activity-dependent tagging system in mice to determine the epigenetic state, 3D genome architecture and transcriptional landscape of engram cells over the lifespan of memory formation and recall. Our findings reveal that memory encoding leads to an epigenetic priming event, marked by increased accessibility of enhancers without the corresponding transcriptional changes. Memory consolidation subsequently results in spatial reorganization of large chromatin segments and promoter-enhancer interactions. Finally, with reactivation, engram neurons use a subset of de novo long-range interactions, where primed enhancers are brought in contact with their respective promoters to upregulate genes involved in local protein translation in synaptic compartments. Collectively, our work elucidates the comprehensive transcriptional and epigenomic landscape across the lifespan of memory formation and recall in the hippocampal engram ensemble.

Conflict of interest statement

Competing interests:

Authors declare no competing interests.

Figures

Similar articles

Memory trace interference impairs recall in a mouse model of Alzheimer's disease.

Poll S, Mittag M, Musacchio F, Justus LC, Giovannetti EA, Steffen J, Wagner J, Zohren L, Schoch S, Schmidt B, Jackson WS, Ehninger D, Fuhrmann M. Poll S, et al. Nat Neurosci. 2020 Aug;23(8):952-958. doi: 10.1038/s41593-020-0652-4. Epub 2020 Jun 8. Nat Neurosci. 2020. PMID: 32514139

Cell-type specific transcriptional adaptations of nucleus accumbens interneurons to amphetamine.

Gallegos DA, Minto M, Liu F, Hazlett MF, Aryana Yousefzadeh S, Bartelt LC, West AE. Gallegos DA, et al. Mol Psychiatry. 2022 Feb 16. doi: 10.1038/s41380-022-01466-1. Online ahead of print. Mol Psychiatry. 2022. PMID: 35173267

References

Alberini CM Transcription Factors in Long-Term Memory and Synaptic Plasticity. Physiol. Rev 89, 1-46 (2014). - PMC - PubMed

Josselyn SA, Köhler S & Frankland PW Finding the engram. Nat. Rev. Neurosci 16, 521-534 (2015). - PubMed

Albo Z & Gräff J Engram Excitement. Neuron 101, 198-200 (2019). - PubMed

Poo M. ming et al. What is memory? The present state of the engram. BMC Biol (2016). doi:10.1186/s12915-016-0261-6 - DOI - PMC - PubMed

Guenthner CJ, Miyamichi K, Yang HH, Heller HC & Luo L Permanent genetic access to transiently active neurons via TRAP: Targeted recombination in active populations. Neuron 78, 773-784 (2013). - PMC - PubMed

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Mapping the epigenomic and transcriptomic interplay during memory formation and recall in the hippocampal engram ensemble - PubMed

# data[].attributes.attachment_link.description
The epigenome and three-dimensional (3D) genomic architecture are emerging as key factors in the dynamic regulation of different transcriptional programs required for neuronal functions. In this study, we used an activity-dependent tagging system in mice to determine the epigenetic state, 3D genome ...

# data[].attributes.headline
기억유전자 관련 논문 

# data[].attributes.subject
기억유전자 관련 논문 

# data[].attributes.attachment_link.title
六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발현에 미치는 영향

# data[].attributes.attachment_link.description
Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed:11303025, PubMed:15695821, PubMed:22412986, PubMed:25326418, PubMed:32433612).

# data[].attributes.body
https://pubmed.ncbi.nlm.nih.gov/26280931/

# data[].attributes.attachment_link.readable_text
The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Free article

Free article

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storage, and have avoided the question of how individual memories are stored in the brain. Memory engram technology allows the labeling and subsequent manipulation of components of specific memory engrams in particular brain regions, and it has been established that cell ensembles labeled by this method are both sufficient and necessary for memory recall. Recent research has employed this technology to probe fundamental questions of memory consolidation, differentiating between mechanisms of memory retrieval from the true neurobiology of memory storage.

Similar articles

Synapse-specific representation of the identity of overlapping memory engrams.

Abdou K, Shehata M, Choko K, Nishizono H, Matsuo M, Muramatsu SI, Inokuchi K. Abdou K, et al. Science. 2018 Jun 15;360(6394):1227-1231. doi: 10.1126/science.aat3810. Science. 2018. PMID: 29903972

Cited by

Enhancing Immediate Memory, Potential Learning, and Working Memory with Transcranial Direct Current Stimulation in Healthy Older Adults.

Satorres E, Meléndez JC, Pitarque A, Real E, Abella M, Escudero J. Satorres E, et al. Int J Environ Res Public Health. 2022 Oct 5;19(19):12716. doi: 10.3390/ijerph191912716. Int J Environ Res Public Health. 2022. PMID: 36232016 Free PMC article. Clinical Trial.

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Memory engram storage and retrieval - PubMed

# data[].attributes.attachment_link.description
A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storag ...

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.attachment_link.readable_text
The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Free article

Free article

doi: 10.1016/j.conb.2015.07.009.

Affiliations

¹ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: tonegawa@mit.edu.

² RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

³ RIKEN-MIT Center for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Abstract

A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storage, and have avoided the question of how individual memories are stored in the brain. Memory engram technology allows the labeling and subsequent manipulation of components of specific memory engrams in particular brain regions, and it has been established that cell ensembles labeled by this method are both sufficient and necessary for memory recall. Recent research has employed this technology to probe fundamental questions of memory consolidation, differentiating between mechanisms of memory retrieval from the true neurobiology of memory storage.

Similar articles

Synapse-specific representation of the identity of overlapping memory engrams.

Abdou K, Shehata M, Choko K, Nishizono H, Matsuo M, Muramatsu SI, Inokuchi K. Abdou K, et al. Science. 2018 Jun 15;360(6394):1227-1231. doi: 10.1126/science.aat3810. Science. 2018. PMID: 29903972

Cited by

Enhancing Immediate Memory, Potential Learning, and Working Memory with Transcranial Direct Current Stimulation in Healthy Older Adults.

Satorres E, Meléndez JC, Pitarque A, Real E, Abella M, Escudero J. Satorres E, et al. Int J Environ Res Public Health. 2022 Oct 5;19(19):12716. doi: 10.3390/ijerph191912716. Int J Environ Res Public Health. 2022. PMID: 36232016 Free PMC article. Clinical Trial.

# data[].attributes.attachment_link.keywords[]
national center for biotechnology information

# data[].attributes.attachment_link.title
Memory engram storage and retrieval - PubMed

# data[].attributes.attachment_link.description
A great deal of experimental investment is directed towards questions regarding the mechanisms of memory storage. Such studies have traditionally been restricted to investigation of the anatomical structures, physiological processes, and molecular pathways necessary for the capacity of memory storag ...

# data[].attributes.body
- 또다른 이름 - Interferon regulatory factor 5
- 기능 - 단백질 전사
- 구조 - 나선형 구조
- sequence(서열) 검색 -

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGD
NTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEV
CSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQ
PPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI
SPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFP
SPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNP
IQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVP
VAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPG
AGLGVGQGPWPMHPAGMQ

- Length (길이) - 498
- Mass (Da, 질량) - 56,044
- Isoform 갯수 - 6

# data[].attributes.headline
기억 유전자 관련 논문 - 2 

# data[].attributes.subject
기억 유전자 관련 논문 - 2 

# data[].attributes.body
https://www.sciencedirect.com/science/article/abs/pii/002228369190496S

(구글스칼라로 찾았습니다)

# data[].attributes.attachment_link.title
Characterization of the memory gene dunce of Drosophila melanogaster

# data[].attributes.attachment_link.description
The dunce (dnc) gene of Drosophila melanogaster encodes cAMP phosphodiesterase (PDEase) and is required for learning/memory and female fertility. The …

# data[].attributes.body
또 다른 이름 - Interferon regulatory factor 5
기능 -
구조 -
Isoform 개수 - 6개
길이: 498
질량: 56,044

# data[].attributes.headline
기억 유전자 관련 논문-구글 스칼라

# data[].attributes.subject
기억 유전자 관련 논문-구글 스칼라

# data[].attributes.attachment_link.readable_text
1: Tonegawa S, Pignatelli M, Roy DS, Ryan TJ. Memory engram storage and
retrieval. Curr Opin Neurobiol. 2015 Dec;35:101-9. doi:
10.1016/j.conb.2015.07.009. Epub 2015 Aug 14. PMID: 26280931.

# data[].attributes.headline
기억 유전자 관련 논문

# data[].attributes.subject
기억 유전자 관련 논문

# data[].attributes.body
https://pubmed.ncbi.nlm.nih.gov/23888038/

# data[].attributes.attachment_link.title
Creating a false memory in the hippocampus - PubMed

# data[].attributes.attachment_link.description
Memories can be unreliable. We created a false memory in mice by optogenetically manipulating memory engram-bearing cells in the hippocampus. Dentate gyrus (DG) or CA1 neurons activated by exposure to a particular context were labeled with channelrhodopsin-2. These neurons were later optically react…

# data[].attributes.body
구글 학술검색

# data[].attributes.body
https://www.sciencedirect.com/science/article/abs/pii/002228369190496S

# data[].attributes.attachment_link.title
Characterization of the memory gene dunce of Drosophila melanogaster

# data[].attributes.attachment_link.description
The dunce (dnc) gene of Drosophila melanogaster encodes cAMP phosphodiesterase (PDEase) and is required for learning/memory and female fertility. The …

# data[].attributes.attachment_link.readable_text
1: Liu X, Ramirez S, Pang PT, Puryear CB, Govindarajan A, Deisseroth K, Tonegawa
S. Optogenetic stimulation of a hippocampal engram activates fear memory recall.
Nature. 2012 Mar 22;484(7394):381-5. doi: 10.1038/nature11028. PMID: 22441246;
PMCID: PMC3331914.

# data[].attributes.body
acession number : E01216.1
FASTA :

ATCGATGTTAACAGATCTAAGCTCCCGGGAGCTTAACTAACTAACTCCGGAAAAGGAGGAACTTCCATGA
TCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC
TGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAA
TCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCG
TAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGG
CGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAA
GTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACG
ATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATT
TACGGCTAGCGCCGGATGCGACGCCGGTCCGGTCTTATCCGGCCTATATCAGGCTGTGTTTAAGACGCGC
CTTCGCCCAAATCCTTATGCCGGTTCGACGGCTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGT
TAATGTACCCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAG
CGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTC
AGTGGGGCAGGGGCTCTTCCGGCGATCTTTCGGTTTATTGCGGCTGATCCCGGCCAAATACCGCCAGCAG
ATGCAGATCGTGATTGCCCAGCACCAGACGTACGCAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTA
ATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTAT
TTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCAT
TTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGC
ACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAG
AGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCA
TCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATG
TAACTCGCCTTGATCGTTGGGAACCGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGC
TTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAG
CCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGA
CGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTC
GCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGG
GACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGC
GATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTC
TGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCT
CGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACC
AACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCG
TTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTG
CCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCT
GCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAG
CGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATC
TGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGT
TGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCT
CTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATTCCCCCTTACACGGAGGCATCAAGTGACCAAA
CAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCA
ACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCG
CAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTC
GGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAG
CAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCA
TCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCA
AAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCA
GGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCC
GCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA
CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATT
AGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATC
CGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAA
GGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAA
ATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATC
TCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGG
AGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATC
AGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAG
TCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCA
TTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATC
AAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC
AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGC
CATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCG
ACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTC
ATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGT
AACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAAC
AGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTT
TTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGA
AAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTAT
TATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAATTGTTATC
CGCTCACAATTAATTCTTGACAATTAGTTAACTATTTGTTATAATGTATTCATAAGCTTCGACGAGATTT
TCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATCGC
ATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGA
TATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTT
GCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATA
GTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCA
CGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTAT
TTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGACTTTCACCAGTTTTG
ATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGG
CGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGA
ATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGGCGGGCGTAATTTTTTTAAGGCAGTTATT
GGTGCCCTTAAACGCCTGGTGCTACGCCTGAATAAGTGATAATAAGCGGATGAATGGCAGAAATTCGTCG
AAGCTT

# data[].attributes.body
"단백질 인산화 효소 C 알파, protein kinase C-alpha, PKCa"
( 사람, Homo sapines, Human ) 인지 확인할 것.

→ FASTA 파일 얻기
→ 엑스파시 툴에 있는 Netphos 링크 선택
https://services.healthtech.dtu.dk/service.php?NetPhos-3.1

→ FASTA 를 이용하여 프로그램 진행
→ 결과 예측 : 예측 인산화 자리는 "Y"로 표시됨
→ 예측한 결과 화면 캡처
→ 패들렛에 업로드

# data[].attributes.attachment_link.readable_text
The NetPhos 3.1 server predicts serine, threonine or tyrosine phosphorylation sites in eukaryotic proteins using ensembles of neural networks. Both generic and kinase specific predictions are performed. The generic predictions are identical to the predictions performed by NetPhos 2.0. The kinase specific predictions are identical to the predictions by NetPhosK 1.0. Predictions are made for the following 17 kinases:

# data[].attributes.attachment_link.description
The NetPhos 3.1 server predicts serine, threonine or tyrosine phosphorylation sites in eukaryotic proteins using ensembles of neural networks. Both generic and kinase specific predictions are performed. The generic predictions are identical to the predictions performed by NetPhos 2.0. The kinase specific predictions are identical to the predictions by NetPhosK 1.0.

# data[].attributes.body
-유전성 유방암 난소암 유발 유전자
-BRCA
-GenBank: MF945608.1
-서열: (BRCA1)

CTCACTAAAGACAGAATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTTAGCAA
GGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCACAGAAAA
AAAGGTAGATCTGAATGCTGATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCA
GAGAATCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTCAGAAAG

(https://www.ncbi.nlm.nih.gov/nuccore/MF945608.1?report=fasta)

# data[].attributes.body
ATGAATTCAACATTATTTTCCCAGGTTGAAAATCATTCAGTCCACTCTAATTTCTCAGAGAAGAATGCCC
AGCTTCTGGCTTTTGAAAATGATGATTGTCATCTGCCCTTGGCCATGATATTTACCTTAGCTCTTGCTTA
TGGAGCTGTGATCATTCTTGGTGTCTCTGGAAACCTGGCCTTGATCATAATCATCTTGAAACAAAAGGAG
ATGAGAAATGTTACCAACATCCTGATTGTGAACCTTTCCTTCTCAGACTTGCTTGTTGCCATCATGTGTC
TCCCCTTTACATTTGTCTACACATTAATGGACCACTGGGTCTTTGGTGAGGCGATGTGTAAGTTGAATCC
TTTTGTGCAATGTGTTTCAATCACTGTGTCCATTTTCTCTCTGGTTCTCATTGCTGTGGAACGACATCAG
CTGATAATCAACCCTCGAGGGTGGAGACCAAATAATAGACATGCTTATGTAGGTATTGCTGTGATTTGGG
TCCTTGCTGTGGCTTCTTCTTTGCCTTTCCTGATCTACCAAGTAATGACTGATGAGCCGTTCCAAAATGT
AACACTTGATGCGTACAAAGACAAATACGTGTGCTTTGATCAATTTCCATCGGACTCTCATAGGTTGTCT
TATACCACTCTCCTCTTGGTGCTGCAGTATTTTGGTCCACTTTGTTTTATATTTATTTGCTACTTCAAGA
TATATATACGCCTAAAAAGGAGAAACAACATGATGGACAAGATGAGAGACAATAAGTACAGGTCCAGTGA
AACCAAAAGAATCAATATCATGCTGCTCTCCATTGTGGTAGCATTTGCAGTCTGCTGGCTCCCTCTTACC
ATCTTTAACACTGTGTTTGATTGGAATCATCAGATCATTGCTACCTGCAACCACAATCTGTTATTCCTGC
TCTGCCACCTCACAGCAATGATATCCACTTGTGTCAACCCCATATTTTATGGGTTCCTGAACAAAAACTT
CCAGAGAGACTTGCAGTTCTTCTTCAACTTTTGTGATTTCCGGTCTCGGGATGATGATTATGAAACAATA
GCCATGTCCACGATGCACACAGATGTTTCCAAAACTTCTTTGAAGCAAGCAAGCCCAGTCGCATTTAAAA
AAATCAACAACAATGATGATAATGAAAAAATCTGA