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is a biological catalysts that speed up the chemical reaction by lowering the activation energy of the reaction without changing themselves through out the process

mostly are proteins

it catalyze specific reaction

it will denature in high temperature

exoenzyme or extracellular enzyme :is an enzyme that is secreted by a cell and functions outside of that cell

endoenzyme/intracellular enzyme:is an enzyme that functions within the cell in which it was produced

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Exoenzyme from Bacillus spp.

Protease

1. Bacillus species are the main producers of extra-cellular proteases, and industrial sectors frequently use Bacillus subtilis for the production of various enzymes.

2. B. subtilis is widely used for the productionof specific chemicals and industrial enzymes.

Link: http://www.sciencedirect.com/science/article/pii/S1658365514000697

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Exoenzyme from  Bacillus spp.

Alpha amylase

1. catalyzes the breakdown of starch into sugars
2. role in a variety of areas like use as for the production of ethanol and high fructose corn syrup, and paper recycling
3. solid state fermentation (SSF) for α-amylase production has been used instead of submerged fermentation (SmF) due to its simple technique, low capital investment and better product recovery.

Link:
http://www.hindawi.com/journals/bri/2014/568141/

http://pubs.sciepub.com/jaem/2/4/10/

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Exoenzyme from  Bacillus spp.

Phytase

1. Phytase increased the availability of phosphorus and other nutrients in farmed animals.
2. In the present study, the phytase-producing ability of the fish gut detected might expand the scope of their use to improve nutrient utilization in fish or other animals.

Link: http://docsdrive.com/pdfs/ansinet/jbs/2013/691-700.pdf

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Coenzymes are synthesized from vitamins.

Most vitamins are needed to form coenzyme, in which it is an organic molecule needed for enzyme activity.

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2 types:

1. inorganic ion

2. coenzyme  (organic molecule)A nonprotein component of enzyme necessary for enzyme proper functioning.

A substance that is essential for the activity of enzyme.

Inorganic substances required to increase the rate of reaction.

Bind to the active site of enzyme but not considered as substrate.

Usually in the form of ions

Cofactor also known as metal ions. In some enzymes, the function as catalyst cannot be carried out if a metal ion is not available to be bound the active site.

In some bacteria such as Azotobacter and Pyrococcus furiosus, metal cofactors are also discovered to play an in important role such as the magnesium-mediated function of restriction endonuclease.