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2017-18 Pyruvate kinase calculation workshop v0

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Describe and discuss
the use of CRISPR gene editing technique and how it might be used in biomedical
sciences

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Describe and discuss
the use of CRISPR gene editing technique and how it might be used in biomedical
sciences

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genetically edited cell lines for research on the following:

1.       Gene function studies. Comparing GMOs to control organisms

2.       Signalling pathway studies. Q. Eliminating or altering a gene that translates to a signalling protein that is likely to be involved in more than one pathway to observe the effect on cell vitality, function and phenotype. Altering the gene rather than eliminating it would allow to study the effect of elimination of it on one or a fraction of pathways it is involved in.

3.      Q. Epigenetics (wrong?)

4.       To produce GMOs with a muation/s for Disease development research which leads to  Drug development where an appropriate intervention point is found.

6.       Gene editing /therapy- replacement of a gene or a section of a DNA strand as synthetic gRNA can be of any sequence and length as long as its compatible with the cas9 endonuclease by its shape/sequence (pins and fplds)

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Use of CRISPR/Cas system for gene editing

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Use of CRISPR/Cas system for gene editing

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Basic information on: genetics and Clustered Regularly Interspaced Short Palindromic Repeats 

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Basic information on: genetics and Clustered Regularly Interspaced Short Palindromic Repeats 

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and associated RNA-guided DNA endonuclease (Cas)

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A video of Jennifer Doudna explaining her findings

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A video of Jennifer Doudna explaining her findings

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https://www.youtube.com/watch?v=SuAxDVBt7kQ&feature=share

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Inhibition by anti-CRISPR protein- possible antidote to gene editing in organisms?

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Inhibition by anti-CRISPR protein- possible antidote to gene editing in organisms?

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Chapters: 1, 4, 5, 6, 7

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Links to relevant and current publications available

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Confirmation of method efficiency for gene editing using GFP

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Confirmation of method efficiency for gene editing using GFP