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Mixo cultivation ile ilgili 4.2 kısmı önemli

Mixotrophic cultivation is considered as the most suitable alternativeto improveHaematococcusgrowth and astaxanthin accumulation.214Various  studies  have  reported  the  superior  performance  ofmixotrophic cultivation ofH.  pluvialisfor astaxanthin production.215–220Nevertheless, the production of astaxanthin fromH. pluvialisis stillvery challenging due to high production cost, low growth rate, highrisk of contamination and extended cultivation period

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-- The experimental units were then inoculated from an exponentially growing culture with an initial cell density of 10 × 104 cells/mL. All the flasks were kept at a temperature of 24 ± 1 °C, constant aeration (flow rate of 2 L/min), and under an irradiance of 60 μmol photons/m2/s (fluorescent lamps) with continuous illumination.
--Table 1. shows all the composition of all medium.
--The determination of astaxanthin followed the extraction method with hydrochloric acid pretreatment followed by acetone extraction (HCl-ACE) described by Dong et al. (Dong et al. 2014), and the extract obtained was filtered in filter 0.22 μm and analyzed by LC-MS
--. Different sources of nitrogen (sodium nitrate, ammonium chloride, and urea) on the growth in Haematococcus demonstrated the best results with sodium nitrate (Mazumdar et al. 2019).
--Higher cell densities (84.50 × 104 cells/mL) and biomass production were observed under mixotrophic conditions in modified Provasoli medium, which besides sodium glycerophosphate, sodium nitrate, and L-asparagine has an addition of yeast extract and organic carbon (sodium acetate)

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Evaluation of the influence of different culture media on growth, life cycle, biochemical composition, and astaxanthin production in Haematococcus pluvialis - Aquaculture International

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Haematococcus pluvialis is known as the richest natural source of carotenoid astaxanthin, whose synthesis is related to changes in cells according its complex life cycle and the availability of nutrients present in the culture media. This study's objective was to evaluate the influence of different culture media under photoautotrophic and mixotrophic conditions on the growth, life cycle, biochemical composition, and astaxanthin production in H.

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Strain H₂-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation

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Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation - Journal of Oceanology and Limnology

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Two strains H2-410 and H2-419 were obtained from the chemically mutated survivors of wild Haematococcus pluvialis 2 by using ethyl methanesulphonate (EMS). Strains H2-410 and H2-419 showed a fast cell growth with 13% and 20% increase in biomass compared to wild type, respectively.

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Log fazında üretildikten sonra kimyasallar yardımıyla mutajeneze uğruyor asta biriktirmesi için uzun ve biraz kompleks koşullar var.

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luvialis NIES-144, which was obtained from the National Institute for Environmental Studies (NIES, Tsukuba, Japan). The seeds were maintained in 250 mL flasks in modified sterilized kobayashi’s basal medium at a constant temperature of 25 ± 1 °C under light intensity of 4±1 μmol photons m−2 s −1 , provided by cool white fluorescent tubes with constant lighting. The modified medium contained 1.2 g CH3COONa, 2.0 g yeast extracts, 0.405 g L-asparagine, 0.2 g MgCl2·6H2O, 0.01 g FeSO4·7H2O, 0.02 g CaCl2·2H2O and 1000 mL distilled water (Kobayashi et al., 2001). The seeds were cultivated until the late exponential growth phase at biomass of 1.0 g L−1 approximately, then harvested by centrifugation in the speed of 3900 rpm for 5 min, and the algal pellets were washed twice by fresh medium for inoculation.

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Sci-Hub | A novel fed-batch strategy to boost cyst cells production based on the understanding of intracellular carbon and nitrogen metabolism in Haematococcus pluvialis. Bioresource Technology, 121744 | 10.1016/j.biortech.2019.121744

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Espacenet: free access to millions of patent documents. Find out if your invention is unique or if other inventors have filed patent applications that are considered to be prior art.

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Min-Eui Hong, Yoon Young Choi and Sang Jun Sim

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Effect of inducing agents on growth and astaxanthin production in Haematococcus pluvialis: Organic and inorganic

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Fig. 1 önemli

--The strain was grown photoautotrophically in NIES-C medium (pH 7.5) consisting of (in gram per liter unless otherwise stated): Ca(NO 3)2 0.15, KNO 3 0.10, βglycerophosphoric acid disodium salt pentahydrate 0.05, MgSO 4

·7H

2

O 0.04,

Tris-aminomethane 0.50, thiamine 0.01 mg l

−

1

, PIV metal

solution 3.00 ml l

−

1

, biotin 0.10

μ

gl

−

1

, and vitamin B

12

0.10

μ

gl

−

1

. The formulation of PIV metal solution was

Na

2

EDTA 1.0, FeCl

3

·6H

2

O 0.196 and (in milligram per

liter) MnCl

2

·4H

2

O 36.0, ZnSO

4

·7H

2

O 22.0, CoCl

2

·6H

2

O

4.0, and Na

2

MoO

4

·2H

2

O 2.5 (Hata et al.

2001

).

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Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis: C/N ratio and light intensity | EndNote Click

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Download PDF of Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis: C/N ratio and light intensity published in Applied Microbiology and Biotechnology

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Fig 2 önemli

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Algal Culture. H. pluVial from Sammlung von Algenkulturen, Pflanzen Physiologisches Institut, Universitat Gottingen, Gottingen, Germany, and grown in an autotrophic medium as detailed in Tripathi et al. (13). The cultures were incubated at 25 (1 °C under a light:dark (16:8 h) cycle with 1.5 klux intensity for aperiod of 1 week. Later, the cultures were incubated at a continuouslight intensity of 3.0 klux with the addition of 0.2% NaCl and 4.4 mM sodium acetate for a period of 2 weeks. The encysted cells whichappeared red due to accumulation of carotenoids were harvested bycentrifugation at 5000 rpm for 5 min at 4 °C. Harvested biomass was lyophilized and used for extraction purposes.

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Algal Culture. H. pluVial from Sammlung von Algenkulturen, Pflanzen Physiologisches Institut, Universitat Gottingen, Gottingen, Germany, and grown in an autotrophic medium as detailed in Tripathi et al. (13). The cultures were incubated at 25 (1 °C under a light:dark (16:8 h) cycle with 1.5 klux intensity for aperiod of 1 week. Later, the cultures were incubated at a continuouslight intensity of 3.0 klux with the addition of 0.2% NaCl and 4.4 mM sodium acetate for a period of 2 weeks. The encysted cells whichappeared red due to accumulation of carotenoids were harvested bycentrifugation at 5000 rpm for 5 min at 4 °C. Harvested biomass was lyophilized and used for extraction purposes.

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fig 1lere bak.