# data[].attributes.body
Use of invivo model

The mechanism of interaction of alpha glucan and the host through PRRs

Quantify dectin in protein level after shRNa

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Use of immunofluorescencw microscopy to quantify and localise beta and alpha glucans critically

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Use of immunofluorescencw microscopy to quantify and localise beta and alpha glucans critically

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a-(1,3)-glucan interferes with recognition of dectin-1 and TNFa production

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a-(1,3)-glucan interferes with recognition of dectin-1 and TNFa production

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TNFa production was reduced to similar levels by KD of dectin-1 in host or by presence of a-(1,3)-glucan

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Alpha glucans masks beta glucan from dectin receptors and thereby don't activate immune cascade 

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Alpha glucans masks beta glucan from dectin receptors and thereby don't activate immune cascade 

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Study functional significance of a-(1,3)-glucan masking by examining TNFa production

# data[].attributes.subject
Study functional significance of a-(1,3)-glucan masking by examining TNFa production

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KO of a-(1,3)glucan in yeast cells

KD of dectin-1 in P388D1 macrophage-like cells and infect with WT and KO yeasts

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Immunofl microscopy to localise the position of alpha glucan

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Immunofl microscopy to localise the position of alpha glucan

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Labelling with gold particle

Ags1 KO and binding with 3T3 cells dectin +/-

Tnf alpha release by P388D1 phagocytes

ShRNA to reduce expression of both isoforms  of dectin 1 and its role in mediating immune recognition

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Mechanism of vriulence mediated by alpha glucan

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Mechanism of vriulence mediated by alpha glucan

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a-(1,3)-Glucan blocks recognition by dectin-1

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a-(1,3)-Glucan blocks recognition by dectin-1

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ags1 KO binds to 3T3 cell expressing dectin-1 and dectin-1 localization with antibody labeling

Verified and quantified using IF Microscopy

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Study the interaction of dectin-1 and a-(1,3)-glucan

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Study the interaction of dectin-1 and a-(1,3)-glucan

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Express dectin-1 transgene in 3T3 fibroblast cell line and treat cell lines with yeast cells expressing a-(1,3)-glucan and without.

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To find out how a-(1,3)-glucan contributes to virulence

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1) A-(1,3)-glucans reduces dectin-1 recognition of histoplasma yeast, recognition based solely on dectin-1
2) a-(1,3)-glucans suppresses the release of tnf-a by phagocytic cells, reduces activation of macrophages, and reduces phagocytosis of the histoplasma

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Immunolabeled IF Microscopy and Electron Microscopy to verify colocalization of a-(1,3)-Glucan and b-(1,3)-Glucan

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Immunolabeled IF Microscopy and Electron Microscopy to verify colocalization of a-(1,3)-Glucan and b-(1,3)-Glucan

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a-(1,3)-Glucan abt 20-100nm away from cell membrane
b-(1,3)-Glucan abt 0-40nm away from cell membrane

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Verify location of a-(1,3)-Glucan around yeast buds using DIC and IF microscopy using labelled antibody

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Verify location of a-(1,3)-Glucan around yeast buds using DIC and IF microscopy using labelled antibody

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Histoplasma cell wall a-(1,3)-glucan blocks PRR recognition and facilitates evasion of host detection