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Techniques choisies :
- Electrophorèse SDS-page et chromatographie HPLC.

Domaine d'application choisi : Génétique et analyse phytochimique.

Mots clés : Protein analysis, genetic fidelity, regeneration, phytochemistry.

Référence Science Direct :

Khattab S., El Sherif F., El-Garhy Hoda A., (2014)

Genetic and phytochemical analysis of the in vitro regenerated Pilosocereus robinii by ISSR, SDS–PAGE and HPLC. Gene. 553 :313–321

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profiling and quantifying quercetin glucosides in onion varieteies

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RAVELO PEREZ L. M., HERNANDEZ BORGES J., RODRIGUEZ DELGADO M.A., (2006), Pesticides analysis by liquid chromatography and capillary electrophoresis. Journal of separation science. 29 ; 2557-2577.

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determination of flavonoids by high performance liquid chromatography and capillary electrophoresis

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Nutrition santé:
Chromatography

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http://www.sciencedirect.com.passerelle.univ-rennes1.fr/science/article/pii/S037811191301216X?np=y&npKey=70c1e253d36b519806304462158221c725975b120de1e574b03f158135b63e0c

References :

Khattab S., El Sherif F., El-Garhy Hoda A., (2014)

Genetic and phytochemical analysis of the in vitro regenerated Pilosocereus robinii by ISSR, SDS–PAGE and HPLC. Gene. 553 : 313–321

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Chromatography, electrophoresis, HPLC, food analysis, CCM

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CCM
Electrophoresis
Plant - Aloe Vera
Cosmetic

Pal J.K., Godbole D., Sharma K. (2004) Staining of proteins on SDS polyacrylamide gels and on nitrocellulose membranes by Alta, a colour used as a cosmetic. Journal of biochemical and biophysical methods. 61 : 339-347

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HPLC ; PAGE-SDS ; Couplage avec RMN et/ou spectro de masse ; Enzymatic characterization ; purification steps.

Reference :

1. Gotti R., Fiori J., Bartolini M., Cavrini M. (2006) Analysis of Amaryllidaceae alkaloids from Narcissus by GC–MS and capillary electrophoresis. Journal of pharmaceutical and biomedical analysis. 42(1) : 17-24.

2. Shivalingu B. R., Vivek H. K., Priya B. S., Soujanya K. N., Swamy NS. N. (2016) Purification and characterization of novel fibrin(ogen)olytic protease
from Curcuma aromatica Salisb.: Role in hemostasis. Phytomedicine. 23 : 1691-1698.

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1. Analysis of flavonoides in Rubus erythrocladus and morus nigra leaves extracts by liquid chromatography and capillary electrophoresis (2015)

2. Comparaison off microbial communities between normal and swollen canned soy sauces using nested PCR denaturing gradient gel electrophoresis, HPLC and plate techniques
Référence : Cheng L., Lin W., Li P., Huang J., Luo L. (2014) Comparison of microbial communities between normal and swollen canned soy sauces using nested PCR-denaturing gradient gel electrophoresis, HPLC and plate techniques. Food Science & Technology. 49 : 2499–2505

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