# data[].attributes.headline
	
	
	
	
	
		 Effect of Energy Treatment on Carrots
	

# data[].attributes.subject
	
	
	
	
	
		 Effect of Energy Treatment on Carrots
	

# data[].attributes.body
This research shows the impact of unique energy treatment on the yield, quality and control of Nematodes in Carrots. For more details visit the link.

# data[].attributes.attachment_link.title
Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots | Mahendra Trivedi | Vishal D Shinde | Shrikant Patil | Academic Room

# data[].attributes.attachment_link.description
This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on carrot crops, Daucus carota , under typical growing conditions in year 2012 at Guadalupe, California, USA.

# data[].attributes.headline
	
	
	
	
	
		Carrots Yield Improvement
	

# data[].attributes.subject
	
	
	
	
	
		Carrots Yield Improvement
	

# data[].attributes.body
This research tested the effect of unique human energy treatment when used for control of nematode on carrot crops, Daucus carota, under typical growing conditions.

# data[].attributes.headline
	
	
	
	
	
		Energy Impact on Immunity of Carrots
	

# data[].attributes.subject
	
	
	
	
	
		Energy Impact on Immunity of Carrots
	

# data[].attributes.body
In the present research, the biofield energy treatment has resulted in an improvement in the overall immunity of Carrots as compared to untreated crops.

# data[].attributes.attachment_link.title
"Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on carrot crops, Daucus carota, under typical growing conditions in year 2012 at Guadalupe, California, USA.

# data[].attributes.headline
	
	
	
	
	 Increase in Vitamin-A Content in Carrots
	

# data[].attributes.subject
	
	
	
	
	 Increase in Vitamin-A Content in Carrots
	

# data[].attributes.body
The study shows how the unique energy treatment has resulted in an increase of the Vitamin-A content in Carrots, compared to the untreated sample.

# data[].attributes.headline
	
	
	
	
	Nematode Control in Carrots - Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	Nematode Control in Carrots - Biofield Treatment
	

# data[].attributes.body
The study suggests that due to the effect of human energy treatment, an improvement in was observed in Nematode control in carrots. Follow this link for the more details.

# data[].attributes.attachment_link.title
Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots

# data[].attributes.attachment_link.description
This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on carrot crops, Daucus carota, under typical growing conditions in year 2012 at Guadalupe, California, USA. Following biofield treatment,

# data[].attributes.headline
	
	
	
	
	
		 Study of Daucus Carota Yield Improvement
	

# data[].attributes.subject
	
	
	
	
	
		 Study of Daucus Carota Yield Improvement
	

# data[].attributes.body
Read here the current study which focused on to evaluate the impact of human energy treatment on the yield improvement of Daucus Carota.

# data[].attributes.headline
	
	
	
	
	 Daucus Carota Nematode Control
	

# data[].attributes.subject
	
	
	
	
	 Daucus Carota Nematode Control
	

# data[].attributes.body
The current research study shows the effect of unique energy treatment on the improvment of Daucus carota nematode control, yield as well the quality.

# data[].attributes.headline
	
	
	
	
	
		 Analysis of Yield Improvement in Carrots
	

# data[].attributes.subject
	
	
	
	
	
		 Analysis of Yield Improvement in Carrots
	

# data[].attributes.body
The biofield treatment caused the numerical improvement in yield in carrots along with nematode control, however, caused statistically significant increase in Vitamin A content.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Impact of Biofield Treatment on Yield, Quality and Control of Nematode in

1Pacific Ag Research, 1840 Biddle Ranch Road, San Luis Obispo, CA, USA

2Trivedi Global Inc, 10624 S Eastern Avenue Suite A-969 , Henderson, NV, USA

*Corresponding author: Shrikant Patil, Trivedi Global Inc, 10624 S Eastern Avenue Suite A-969, Henderson, NV, USA, Tel: +1 602-531-5400; E-mail:

Copyright:© 2015 Shinde VD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted

use, distribution, and reproduction in any medium, provided the original author and source are credited.

This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on

carrot crops, Daucus carota, under typical growing conditions in year 2012 at Guadalupe, California, USA. Following

biofield treatment, carrot seeds were planted in replicate plots with mechanical seeder and their development was

recorded compared to control seed growth from untreated plots and plots treated with the commercial standard

nematicide, further Vydate L was applied three times at rates of 1 and 0.5 gal/A, at 1, 18 and 35 days after seeding,

respectively. At 70 and 109 days after the first application root galling severity in biofield treated crops was reduced

by 54% and 22% respectively as compared to untreated while the Vydate response showed 0% and 25% control,

respectively. Plots planted with biofield treated seeds resulted in the greatest number and weight of marketable

carrot roots. Total yield and gross return were greatest in biofield treated group producing an approximately 33%

increase over the untreated controls and 15% increase over those treated with the commercial standard, Vydate L.

Vitamin A (beta carotene) was significantly greater (6512 IU/100 g) in biofield treated carrots compared with both the

untreated controls (4941) and the commercial standard (5143). The results concluded that, Biofield treatment

caused the numerical improvement in yield along with nematode control in carrots however, caused statistically

significant increase in Vitamin A content.

Keywords: Biofield treatment;

processes [1]. In recent times, an increasing number of papers are

showing scientifically measurable impact of the biofield energies of

some energy healers transmitted through intentional mental energies

and interacting with matter, microbes and plants. The basic

assumption of science that such energies can be ignored in normal

scientific models of the material world until proved both relevant and

productive has led to the development of many noteworthy and

reliable technologies. Nevertheless, the finding that biofield energies

are able to directly influence matter, and more specifically, that they

can influence the development and self-expression of living organisms,

is of value to science. It is therefore necessary to establish their scope

transformations in the physical and structural properties of organic

and inorganic powder materials due to a biofield energy transmission

technique, also referred herewith as Biofield treatment. They report

that elemental diamond, graphite and activated charcoal powders

structure after the said treatment. Dabhade, et al. [4] similarly show

that measurable changes in particle size and hence surface area as well

as crystallite size of antimony and bismuth metal powders resulted

from the same external biofield treatment which they suggest may

have caused a relatively high energy state to occur within the treated

substances. With regard to plant life, Patil, et al. [5] reported results

from this energy of consciousness on micropropagation response of

Patchouli. The source of energy treatments used in these studies was a

specific technique by an internationally well-known energy

practitioner and his students, whose names are not mentioned here in

provided on demand for replication experiments.

synergistic intention, it may be expected that living materials would be

able to show the impact more effectively. While the impact on humans

experiments reported above show significant

laboratory conditions and in early tissue growth stages. However

testing was able to show more consistent results due to the larger

adaptive challenges faced by samples in the field. Shinde et al. [6] and

Sances, et al. [7] compared the impact of biofield treatment when

applied with and without various scientific treatments of pesticides

and fertilizers which further showed a statistical increase when biofield

is applied along with normal scientific treatments. Thus they showed a

synergistic effect of the biofield treatment with externally applied

reported the soybean productivity was found to be similar when only

biofield treatment was applied at the level of seeds as compared to the

control group, which received standard fungicides application.

Journal of HorticultureShinde et al., J Horticulture 2015, 2:3

http://dx.doi.org/10.4172/2376-0354.1000150

Research ArticleOpen Access

ISSN:2376-0354 Horticulture, an open access journalVolume 2 * Issue 3 * 1000150

Vitamin A is a pale yellow primary alcohol derived from carotene.

It affects the formation and maintenance of skin, mucous membranes,

bones, teeth, vision and reproduction. In addition dietary Vitamin A,

in the form of beta carotene, an antioxidant, may help reduce the risk

of certain cancers. A lot of emphasis has been given on breakdown of

vitamin A content of carrots after processing or cooking, however,

there were hardly any attempts made for improvement of Vitamin A

content while growing carrots at field levels [9].

patterns of Staphylococcus epidermis, Yersinia enterocolitica and on

Enterococcus faecalis [10-12]. The details of several scientific

investigations and the results achieved through Biofield on Vanadium

Pentoxide, Silicon, Tin, Lead, Zirconia, Silica, Aluminium and many

more, in the form of original data are reported elsewhere [13-17].

In this paper we report on comparison of crop quality, vitality,

disease and yield of treated and untreated commercial carrot plantings.

While disease may be effectively controlled using external chemicals, it

is normally seen that subsequent yields are affected by such

nematode control, shows a crop growth response for a few weeks

systemic insecticide which can control feeding damage from some soil

and foliar insects. The objective was to compare the crop performance

with various types of treatments, namely untreated controls, crops

treatment, in a blind randomized study.

Treatments were of three types, with five replicates per treatment.

) seeds, untreated in any way, were

planted in untreated plots and in plots treated with the commercial

standard, Vydate L, applied three times at rates of 1 gal/A, 0.5 gal/A,

and 0.5 gal/A at 1, 18 and 35 days after seeding respectively in a

dilution of 27,154 gal/A (one acre-inch of water). Applications were

performed using drip irrigation operated at a pressure of 10 psi. The

performance of these treatments was compared with that of biofield

treated seeds planted in untreated plots. The biofield treatment was

applied for about 3 minutes to the seeds. Plots in the three cases were

allotted for treatment in randomized fashion, with locations unknown

length, along with root galling severity and yield measurements based

on production grade number and weight of marketable carrot roots.

Livermore, CA. Field or laboratory evaluations were conducted six

times over a two month period of growth.

Statistics were analyzed using ANOVA mean comparison with LSD

Crop measurements (Figure 1) showed significantly higher shoot

lengths on plants from Vydate-treated plots as compared to crops

from untreated control and from biofield treated plots. The shoot

lengths in treatment Vydate was significantly higher.

Figure 1: Average shoots length per treatment (cm). DAP_ Days

after planting. a,b. Statistical differences are inclusive.

There appeared to be no signs of insects or insect feeding that

experiments were conducted in the winter months, with evaluations

done from November to January, when most of the pests are in more

dormant stages, showing less infestation of plants. However, when

root galling severity was compared at 70 DAP and at 109 DAP (Figure

biofield-treated and Vydate-treated crops as compared to untreated

controls.

Figure 2: Percent control of root galling severity per treatment.

DAP_Days after planting a,b. Statistical differences are inclusive.

The severity was reduced by 54% and 22% respectively in biofield

treated crops; this is compared to the Vydate response of 0% and 25%,

respectively. Thus overall the percent control of root galling severity

per treatment was on average seen to be better in biofield treated

Citation: Shinde VD, Trivedi MK, Patil S (2015) Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots. J Horticulture

2: 150. doi:10.4172/2376-0354.1000150

ISSN:2376-0354 Horticulture, an open access journalVolume 2 * Issue 3 * 1000150

crops, while at the end a comparable result was seen between Vydate-

treated and biofield-treated crops.

Plots planted with Biofield treated seeds resulted in the greatest

number and weight of marketable carrot roots (Figure 3). Total yield

and gross return were greatest for this treatment producing 16,267 lb/

acre at a first point of sale value of $3904/acre (Figure 4). This is

approximately a 33% increase over the untreated controls and a 15%

increase over the commercial standard, Vydate L.

weight of marketable roots and average total yield (lb/acre) per

treatment. a,b . Statistical differences are inclusive.

Vitamin A (beta carotene) content (Figure 5) was significantly

controls as well as the commercial standard, yielding 6512 IU/100 g of

root tissue, which was a 32% increase over untreated controls and a

27% increase over the Vydate-treatment further testifying to improved

Figure 4: Average gross return ($/acre) per treatment. a. Statistical

differences are inclusive.

Figure 5: Average content of beta carotene content per treatment

(IU/100 g). a,b. Statistical differences are inclusive.

improved overall immunity as compared to untreated crops, whereas

growth in terms of shoot length was not improved by the biofield

treatment, the treatment was effective in its targets as an alternative to

the chemical treatment, at the same time providing higher marketable

yields of crop and gross return per acre besides better quality and

higher vitamin A content. The shoot lengths in the plots which are

treated with Vydate was significantly higher, however it was not

directly proportional to the yield since biofield treatment produced

more yields. It seems that Vydate treatment caused more vegetative

growth on top of the soil but biofield treatment enhanced root growth

which eventually produced more yields. In recent years demand of

beta carotene has been increasing steadily because of its possible roles

in the treatment of human diseases [9]. In current studies effect of

biofield treatment found significantly positive on Vitamin A content

in carrots. The results indicated the ability of

to biofield treatment with improvement in yield, quality (vitamin A)

Citation: Shinde VD, Trivedi MK, Patil S (2015) Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots. J Horticulture

2: 150. doi:10.4172/2376-0354.1000150

ISSN:2376-0354 Horticulture, an open access journalVolume 2 * Issue 3 * 1000150

# data[].attributes.attachment_link.title
Impact of Biofield Treatment on Yield, Quality and Control of Nematode in Carrots - PdfSR.com

# data[].attributes.attachment_link.description
This study tested the Null Hypothesis for the effect of biofield treatment when used for control of nematode on carrot crops, Daucus carota, under typical growing conditions in year 2012 at Guadalupe, California, USA.

# data[].attributes.headline
	
	
	
	
	 Enterococcus Faecalis | Biotype Number Analysis
	

# data[].attributes.subject
	
	
	
	
	 Enterococcus Faecalis | Biotype Number Analysis
	

# data[].attributes.body
Under the inflence of a unique spiritual energy treatment by Mr. Mahendra Trivedi the biochemical analysis of Enterococcus Faecalis shows the change in biotype number of blessed samples of enterococcus faecalis.

# data[].attributes.attachment_link.title
Impact of an external energy on Staphylococcus epidermis [ATCC -13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study

# data[].attributes.attachment_link.description
Purpose: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC -13518], validate the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	
		Effect of External Energy on Enterococcus Faecalis
	

# data[].attributes.subject
	
	
	
	
	
		Effect of External Energy on Enterococcus Faecalis
	

# data[].attributes.body
The present experiments on Enterococcus faecalis, report the effect of external energy, transmitted through a person, Mr Mahendra Kumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.attachment_link.keywords[]
e. faecalis in microscan walkaway system

# data[].attributes.attachment_link.keywords[]
biochemical characteristics of e. faecalis

# data[].attributes.attachment_link.keywords[]
antibiotic susceptibility of e. faecalis

# data[].attributes.attachment_link.keywords[]
biotyping characteristics of e. faecalis

# data[].attributes.attachment_link.keywords[]
enzymatic characteristics of e. faecalis

# data[].attributes.attachment_link.title
"Impact of an external energy on Enterococcus faecalis [ATCC - 51299] in relation to antibiotic susceptibility and biochemical reactions - An experimental study" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Background: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC -51299], report the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	Enterococcus Faecalis Biochemical Characteristics
	

# data[].attributes.subject
	
	
	
	
	Enterococcus Faecalis Biochemical Characteristics
	

# data[].attributes.body
Read the current study which shows the impact of human energy treatment on biochemical characteristics of Enterococcus Faecalis.

# data[].attributes.headline
	
	
	
	
	Enterococcus Faecalis' Antibiotic Susceptibility Pattern
	

# data[].attributes.subject
	
	
	
	
	Enterococcus Faecalis' Antibiotic Susceptibility Pattern
	

# data[].attributes.body
The analysis of Enterococcus Faecalis' antibiotic susceptibility pattern shows a significant change in susceptibility pattern in 5 of 31 antibiotics after the influence of human energy treatment.

# data[].attributes.attachment_link.title
Impact of an external energy on Enterococcus faecalis [ATCC - 51299] in relation to antibiotic susceptibility and biochemical reactions - An experimental study

# data[].attributes.attachment_link.description
Background: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC

# data[].attributes.headline
	
	
	
	
	Enterococcus Faecalis' Biochemical Reactions
	

# data[].attributes.subject
	
	
	
	
	Enterococcus Faecalis' Biochemical Reactions
	

# data[].attributes.body
The human energy treated samples of Enterococcus Faecalis were analysed for identification, biochemical reactions and susceptibility testing. Read here for the more details.

# data[].attributes.headline
	
	
	
	
	Alteration in Enterococcus Faecalis Biotype Number
	

# data[].attributes.subject
	
	
	
	
	Alteration in Enterococcus Faecalis Biotype Number
	

# data[].attributes.body
In this research, Enterococcus Faecalis' biotype number have changed in the samples treated with the alternative energy treatment by the 10th day, compared to the control sample.

# data[].attributes.headline
	
	
	
	
	
		 Characteristics of Enterococcus Faecalis
	

# data[].attributes.subject
	
	
	
	
	
		 Characteristics of Enterococcus Faecalis
	

# data[].attributes.body
| The main aim of this study was to evaluate the impact of human energy treatment on the various characteristics of Enterococcus Faecalis.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2009_5_2_impact_of_an_external_energy_on_enterococcus.htm 1/14

JournalofAccordIntegrativeMedicine

Copyright©2009Allrightsreserved.Nopartofthispublicationmaybeusedor

reproduced,storedinaretrievalsystem,ortransmittedinanyformsorbyanymeans,

electronic,mechanical,photocopying,recordingorotherwise,withoutwritten

permission.

ImpactofanexternalenergyonEnterococcusfaecalis

[ATCC-51299]inrelationtoantibioticsusceptibility

andbiochemicalreactions-Anexperimentalstudy

2009,Volume5,Number2:119130

MahendrakumarTrivedi(FounderPresident1,2,FounderChairman3),Dr.Yogi

ShrikantPatil(Associate)1*,HarishShettigar(Trustee2),ArchanaBulbule(Treasurer2)

1SocietyforDivineLife,A14,KanwalApartment,FourBungalows,Andheri(W.),

Mumbai,Maharashtra-INDIA

2AdvancedSocietyForDivineLife,Bhopal(MadhyaPradesh)INDIA

3DivineLifeFoundation,1680,N.DelanyRoad,Gurnee,Illinois-600311238USA

*CorrespondingauthorShrikantPatil

5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2009_5_2_impact_of_an_external_energy_on_enterococcus.htm 2/14

Contactnumber+1(847)8760898

Categoryofsubmission:InVitroStudy

Institutioninwhichtheworkwasperformed:

MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearchCentre,

Mumbai,India-accreditedbyTheCollegeofAmericanPathologists

Keywords-Antibioticresistance,BiochemicalReactions,Lyophilization,

Polymorphism,Energy,Model

Whilespiritualandmentalenergiesareknowntoman,theirimpacthasneverbeen

scientificallymeasurableinthematerialworldandtheyremainoutsidethedomainof

science.ThepresentexperimentsonEnterococcusfaecalis [ATCC-51299],reportthe

effectsofsuchenergytransmittedthroughaperson,Mr.MahendrakumarTrivedi,which

hasproducedanimpactmeasurableinscientificallyrigorousmanner.

Enterococcusfaecalis strainsinrevivedandlyophilizedstateweresubjectedtospiritual

energytransmittedthroughthoughtinterventionand/orphysicaltouchofMr.Trivedito

thesealedtubescontainingstrain,theprocesstakingabout3minutesandwereanalyzed

within10daysafterincubation.Alltestswereperformedwiththehelpofautomationon

theMicroscanWalkawaySysteminMicrobiologyLaboratoryaccreditedbyThe

CollegeofAmericanPathologists

5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2009_5_2_impact_of_an_external_energy_on_enterococcus.htm 3/14

TheresultsindicatedthatMr.Trivedi'senergyhaschanged9of27biochemical

characteristicsofEnterococcusfaecalis alongwithsignificantchangesinsusceptibility

patternin5of31antibiotics.TheBiotypenumberhaschangedfromtheoriginalcontrol

straingivingriseto2differentbiotypesintreatedsampleswhiletheexternalenergy

/treatmentgivenwasthesameforalltreatedsamplessuggestiveofrandom

polymorphismasanalyzedthroughtheautomatedmachine.

Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicateapotency

inMr.Trivedi'senergy,providingamodelforsciencetobeabletoinvestigatethe

impactofspiritualenergyinarigorousmanner.

Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.

Alllivingorganismscontainlargecomplexmoleculeswithinthecells.Someoftheseare

proteinsandenzymesincludingDNA/RNA.Thenatureoftheorganismsisdependent

onthebiochemicalreactions,whichcanbecharacterizedandidentified.

Antibioticresistance,regardlessofantibioticandbacteria,willoccurwithsufficienttime

anddruguse.Widespreadantibioticusecausesselectionpressure:resistantstrains

survivewhilesusceptibleonesareeliminated.Antibioticresistanceisprogressive,

increasingfromlowtointermediatetohighlevels[1].Bacterialstrainsarealso

characterizedbytheirrelativeresistancestoarangeofantibiotics.However,sucha

changedoesnottakeplacespontaneouslyinastrainovera10dayperiodofnormal

incubationwithoutcontinuousexposuretoanydrugs.

# data[].attributes.attachment_link.title
Impact of an external energy on Enterococcus faecalis [ATCC - 51299] in relation to antibiotic susceptibility and biochemical reactions - An experimental study - PdfSR.com

# data[].attributes.attachment_link.description
Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC -51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	Phenotyping and Genotyping Characterization of Serratia marcescens 
	

# data[].attributes.subject
	
	
	
	
	Phenotyping and Genotyping Characterization of Serratia marcescens 
	

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A study was performed to evaluate the impact of biofield treatment on Phenotyping and Genotyping Characterization of Serratia marcescens. Visit here for more details.

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Evaluation of Phenotyping and Genotyping Characterization of Serratia

marcescens after Biofield Treatment

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Bhopal- 462026, Madhya Pradesh, India

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted

use, distribution, and reproduction in any medium, provided the original author and source are credited.

Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections

(HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield

treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions,

biotype, DNA polymorphism, and phylogenetic relationship of S. marcescens (ATCC 13880). The lyophilized cells of

S. marcescens were divided into three groups (G1, G2, and G3). Control group (G1) and treated groups (G2 and

G3) of S. marcescens cells assessed with respect to antimicrobial susceptibility, and biochemical reactions. In

addition to that, samples from different groups of S. marcescens were evaluated for DNA polymorphism by Random

Amplified Polymorphic DNA (RAPD), and 16S rDNA sequencing in order to establish the phylogenetic relationship of

and 34.48% antimicrobials in G2 and G3 on 10th day, respectively as compared to control. The significant changes

of biochemical reactions were also observed in treated groups of S. marcescens. The RAPD data showed an

average range of 16-49.2% of polymorphism in treated samples as compared to control. Based on nucleotide

Pseudomonas fluorescence. These findings suggest that biofield treatment can prevent the emergence of absolute

resistance to the useful antimicrobials against S. marcescens.

Microbial resistance; RAPD;

Currently, many microorganisms have been acquired the resistance

to number of antibiotics and other antimicrobial agents, which were

eectively used earlier to cure a microbial infections. e antimicrobial

resistant microbes (including bacteria, viruses, fungi, and parasites)

antimicrobial medicines accelerates the emergence of drug-resistant

microorganism, which was further spread by meagre infection control

and poor sanitary conditions [1].

is a rod-shaped Gram-negative bacteria, belongs to family

. It is a facultative anaerobic bacterium that can

grow in presence and absence of oxygen at temperatures 30°C to 37°C.

become an opportunist pathogen causing nosocomial

infections and commonly involved in hospital-acquired infections

septicemia, meningitis and wound infections. Recently,

S.

drastically acquired the resistance to several existing antimicrobials

like penicillin by decreasing the permeability and by β-lactamase to

cleave the β-lactam ring of penicillin; uoroquinolones (nalidixic acid,

multidrug resistance (MDR) eux pumps [2,3]. erefore,

development of eective antimicrobial therapy against

very needful for human health. Recently, bioeld treatment came in

focus that can c ure the microbial infect ion by changing the microbial

susceptibility against the antimicrobial drugs.

e relation between mass-energy was described by Friedrich, then

aer Einstein gave the well-known equation E=mc2 for light and mass

[4,5]. e mass (solid matter) is consist of energy and once this energy

vibrates at a certain frequency, it gives physical, atomic and structural

properties like shape, size, texture, crystal structure, and atomic weight

to the matter. Similarly, human body als o consists of vibratory energy

particles like neutrons, protons, and electrons. Due to the vibration of

these particles in the nucleus, an electrical impulse is generated [6].

Consequently, as per Ampere-Maxwell-Law, varying of these electrical

impulses with time generates magnetic eld, which cumulatively form

electromagnetic eld [7,8]. us, human has the ability to harness the

energy from environment or universe and can transmit into any living

or nonliving object(s) around the Globe. e objects always receive the

energy and responding into useful way that is cal led bioeld energy

and the process is known as bioeld treatment. Mr. Mahendra Trivedi's

the structural, physical, and chemical properties of materials in several

elds like material science [9-16], agriculture [17-19], and

the genotype of the microbes and thereby changed in susceptibility to

signicant impact of bioeld treatment on microbes, we felt a detailed

investigation was required to evaluate the eect of bioeld treatment

. Aer that, the organism was assesse d in relation to

Molecular and Genetic MedicineTrivedi et al., J Mol Genet Med 2015, 9:3

http://dx.doi.org/10.4172/1747-0862.1000179

Research ArticleOpen Access

ISSN:1747-0862 JMGM, an open access journalVolume 9 * Issue 3 * 1000179

organism using polymerase chain reaction (PCR) based methodologies

sequencing techniques. To the best of our knowledge, this is the rst

report that explores the impact of bioeld treatment on

[American Type Culture Collection

(ATCC) 13880] were procured from MicroBioLogics, Inc., USA, in

sealed packs, and stored as per the recommended storage conditions

reactions, and biotype number were evaluated on MicroScan Walk-

Away® (Dade Behring Inc., West Sacramento, CA) using Negative

Breakpoint Combo 30 (NBPC30). DNA Fingerprinting by RAPD

analysis (using Ultrapure Genomic DNA Prep Kit; Cat KT 83) and the

Genomic DNA Prep Kit; Cat KT 83 (Bangalore Genei, India). All the

tested antimicrobials, biochemicals and other reagents were procured

e microorganisms were grouped as per study design like bacterial

cell were divided in to three groups G1 (control), G2 (treatment,

revived), and G3 (treatment, lyophilized). e treatment groups (G1

and G2) were in sealed pack and handed over to Mr. Trivedi for

bioeld treatment under laboratory condition. Mr. Trivedi provided

the treatment through his energy transmission process to the treated

assessed for antimicrobial susceptibility and biochemical reactions on

control.

Investigation of antimicrobial susceptibility of

Antimicrobial susceptibility of

the help of automated instrument, MicroScan Walk-Away® using

manufacturer's instructions [25]. Briey, aer inoculation and

rehydration with a standardized suspension of

incubated at 35°C for 16 h. e minimum inhibitory concentration

(MIC) and a qualitative susceptibility like susceptible (S), intermediate

(I), inducible β-lactamases (IB), and resistant (R) were determined by

observing the lowest antimicrobial concentration showing growth

inhibition [26]. In the present study, the following 29 antimicrobials

sulbactam, ampicillin, aztreonam, cefazolin, cefepime, cefotaxime,

piperacillin, tazobactam, ticarcillin, tobramycin, and vancomycin.

e biochemical studies of

MicroScan Walk-Away® where, interpretation of biochemical reactions

for microbial identication of Gram-negative organisms resulted in

high accuracy [27,28]. In this study, the following 31 biochemicals

were used like acetamide, adonitol, arabinose, arginine, cetrimide,

glucose, hydrogen sulde, indole, inositol, kanamycin, lysine,

oxidase, ranose, rhamnose, sorbitol, sucrose, tartrate, tobramycin,

urea, and Voges-Proskauer.

was determined by MicroScan

Walk-Away® processed panel data utilizing biochemical reactions data

Random Amplied Polymorphic DNA (RAPD) analysis

ree inoculums (one for control and other two for treatment

named as treatment A and B) were prepared of

Two inoculums (treatment samples A and B) were subjected to Mr.

Trivedi's bioeld treatment. Aer that, the treated samples were sub-

cultured by taking 1% inoculum and inoculated to fresh 5 mL medium

and labeled as treatment A-1 and treatment B-1, respectively. All

samples were incubated at 37°C with 160 rpm for 18 h. Subsequently,

the cultures were spun down, and genomic DNA was isolated for

control and treated samples using Genomic DNA Prep Kit (Bangalore

Genei, India). RAPD was performed with al l samples of

using ve RAPD primers, which were labelled as RBA8A, RBA13A,

RBA20A, RBA10A and RBA15A. e PCR mixture contained 2.5 µL

each of buer, 4.0 mM each of dNTP, 2.5 μM e ach of primer, 5.0 μL

each of genomic DNA, 2 U each of Taq polymerase, 1.5 μL of MgCl2

and 9.5 μL of water in a total of 25 μL with the following PCR

amplication protocol; initial denaturation at 94°C for 7 min, followed

by 8 cycles of denaturation at 94°C for 1 min, annealing at 35°C for 1

min, and extension at 72°C for 2 min; and 35 cycle of denaturation at

94°C for 1 min, annealing at 38°C for 1 min, and extension at 72°C for

products from all samples (control and treated) were separated on 1.5

visualized under UV illumination.

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from

genomic purication Kit, according to the instructions of

manufacturer. 16S rDNA gene (~1.5 kb) was amplied by universal

Amplied products were subjected to electrophoresis in 1.0% agarose

gel, stained with ethidium bromide and visualized under UV light in a

amplied fragment was puried from the agarose gel using a DNA Gel

sequences obtained were aligned and compared with the sequences

stored in Gene Bank data bas e available from National C enter for

established using MEGA3.1 molecular soware [29].

Assessment of antimicrobial susceptibility

pattern and MIC of selected antimicrobials are summarized in Tables 1

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Evaluation of Phenotyping and Genotyping Characterization of Serratia

ISSN:1747-0862 JMGM, an open access journalVolume 9 * Issue 3 * 1000179

and 2, respectively. e data were analyzed and compared with respect

to control. e treated cells of

10.34% and 34.48% in G2 and G3 group on 10th day, respectively of

compared to control. Studying the eect of bioeld treatment in the

tobramycin were converted from resistance to susceptible on 10th day

ceazidime, cef uroxime and chloramphenicol were converted from

converted from resistance to intermediate on 10th day of G2 treated

cells and complete susceptibility was obser ved for gentamycin and

cefepime on 10th day of G3 treated cells as compared to control (Table

1). It was also observed that there was reduced activity of inducible β-

ceriaxone antimicrobials. e MIC values of amikacin, aztreonam,

decreased about two-folds; whereas about four-folds decrease in MIC

values of cefotaxime and ceriaxone on 10th day of G2 treated cells as

compared to control (Table 2).

S.

G stands for group; I: intermediate; S: susceptible; R: resistant; IB: inducible β-

lactamase.

pattern of selected antimicrobials.

clavulanate ≥16/8≥16/8 ≥16/8 ≥16/8

Sulbactam ≥16/8≥16/8 ≥16/8 ≥16/8

9Cefotetan>32 >32 >32 ≤16

10 Cefoxitin≥16≥16 ≥16 ≥16

11 Ceftazidime>16>16 >16 ≤8

12 Cefuroxime>16>16 >16 >16

14 Cephalothin≥16≥16 ≥16 ≥16

15 Chloramphenicol>16>16 >16 16

16Ciprofloxacin ≤1≤1 ≤1 ≤1

18Gentamicin>8 >8 >8 ≤4

20Levofloxacin ≤2≤2 ≤2 ≤2

22Moxifloxacin ≤2≤2 ≤2 ≤2

23 Nitrofurantoin ≥64≥64 ≥64 ≥64

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Evaluation of Phenotyping and Genotyping Characterization of Serratia

ISSN:1747-0862 JMGM, an open access journalVolume 9 * Issue 3 * 1000179

# data[].attributes.attachment_link.title
Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions, biotype, DNA polymorphism, and phylogenetic relationship of S.

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		Human Energy Effect of 16S rDNA Sequence of S. Marcescens
	

# data[].attributes.subject
	
	
	
	
	
		Human Energy Effect of 16S rDNA Sequence of S. Marcescens
	

# data[].attributes.body
The present research was used to evaluate the impact of human energy treatment of 16S rDNA Sequence of S. Marcescens on the of treated sample as compared to the untreated.

# data[].attributes.attachment_link.title
"Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions, biotype, DNA polymorphism, and phylogenetic relationship of S.

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		Evaluation of Serratia Marcescens- Trivedi Effect
	

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		Evaluation of Serratia Marcescens- Trivedi Effect
	

# data[].attributes.body
The study is based on the effect of Trivedi Effect which has resulted in alteration ofcharacteristics of Serratia Marcescens. Follow the given link.

# data[].attributes.headline
	
	
	
	
	
		Study of Drug-Resistant Microorganism of S. marcescens
	

# data[].attributes.subject
	
	
	
	
	
		Study of Drug-Resistant Microorganism of S. marcescens
	

# data[].attributes.body
Read here the impact of biofield treatment on Drug-resistant Microorganism of S. marcescens. A detailed study was conducted to evaluate the impact of biofield treatment on different characteristics.

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		Study of DNA  Polymorphism of S. Marcescens
	

# data[].attributes.subject
	
	
	
	
	
		Study of DNA  Polymorphism of S. Marcescens
	

# data[].attributes.body
In this research, DNA polymorphism of S. marcescens found that the level of polymorphism was about an average range of 16-49.2% of polymorphism in treated samples as compared to control in S. marcescens.

# data[].attributes.attachment_link.title
Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment

# data[].attributes.attachment_link.description
Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on

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		Analysis  of Microbial Resistance on Serratia Marcescens
	

# data[].attributes.subject
	
	
	
	
	
		Analysis  of Microbial Resistance on Serratia Marcescens
	

# data[].attributes.body
The study of Microbial Resistance on Serratia marcescens determine that the human energy treatment improved the Phenotyping and Genotyping characterization.

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		Impact of Biofield Treatment on Antimicrobial Susceptibility of S. marcescens
	

# data[].attributes.subject
	
	
	
	
	
		Impact of Biofield Treatment on Antimicrobial Susceptibility of S. marcescens
	

# data[].attributes.body
The impact of biofield treatment on antimicrobial susceptibility of S. marcescens shows that there was improved the sensitivity of antimicrobials against microbial resistance.

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		Biofield Treatment’s impact on Salmonella Paratyphi
	

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		Biofield Treatment’s impact on Salmonella Paratyphi
	

# data[].attributes.body
Mr. Trivedi’s biofield treatment could be applied as an alternative therapeutic approach to alter the sensitivity pattern of antimicrobials. Get to know more from the below given link.

# data[].attributes.attachment_link.title
Peer Evaluation : Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Abstract : Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S.

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	Minimum Inhibitory Concentration | S. Paratyphi A
	

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	Minimum Inhibitory Concentration | S. Paratyphi A
	

# data[].attributes.body
In the research, the MIC value of salmonella paratyphi showed a significant alteration of (53.12%) in the treated sample w.r.t the control sample.

# data[].attributes.attachment_link.title
Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S.

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	 Impact of Biofield treatment on Salmonella Paratyphi
	

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	 Impact of Biofield treatment on Salmonella Paratyphi
	

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The objective of this research is to evaluate the impact of biofield treatment on the characteristics of salmonella paratyphi. Visit the link for further details.

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Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping

of Salmonella paratyphi A: An Impact of Biofield Treatment

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal-462026, Madhya Pradesh, India, Tel: +91-755-6660006; E-mail: [email protected]

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted

use, distribution, and reproduction in any medium, provided the original author and source are credited.

Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current

treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella

paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping.

S. paratyphi A strain were procured from MicroBioLogics in sealed packs bearing the American Type Culture

Collection (ATCC 9150). The study was conducted in revived and lyophilized state of S. paratyphi A. Both revived

(Group; Gr. II) and lyophilized (Gr. III) strain of S. paratyphi A were subjected to Mr. Trivedi's biofield treatment.

Revived treated cells was assessed on day 5 and day 10, while lyophilized treated cells assessed on day 10 after

biofield treatment with respect to control (Gr. I). The antimicrobial susceptibility of S. paratyphi A showed significant

(60%) alteration in revived treated cells (Gr. II) on day 10 as compared to control. The MIC values of S. paratyphi A

also showed significant (53.12%) alteration in Gr. II and on day 10 while, no alteration was found in Gr. on day 5 as

compared to control. It was observed that overall 18.18% biochemical reactions were altered in the treated groups

with respect to control. Moreover, biotype numbers were substantially changed in Gr. II, on day 5 (53001040, S.

paratyphi A), on day 10 (57101050, Citrobacter freundii complex) as compared to control (53001000, S. paratyphi

A). Besides, biotype number was also changed in Gr. III (53001040, S. paratyphi A) as compared to control. The

overall result suggested that biofield treatment had significant impact on S. paratyphi A in Gr. II on day 10 with

respect to antimicrobial susceptibility, MIC values and biotype number.

susceptibility; Biofield treatment; Biochemical reaction; Biotype

MIC: Minimum Inhibitory Concentration; ATCC: American Type

Culture Collection; NBPC 30: Negative Breakpoint Combo 30

Enteric fever is a major public health problem in India.

) is motile, non-lactose fermenting, non-spore

forming, and Gram-negative rod shape bacterium. It can ferment

glucose with production of acid and gas. The subspecies of

A, B, and C that cause paratyphoid

fever [1]. The important virulence factor of

S.

A) is cytolysin A i.e. a cytotoxic protein which form pores in

the cell membrane [2]. Salmonellae mainly causes infection through

contaminated food or drink to the gut first through this protein then

enter into bloodstream. After that, it can infect to other parts of the

body. Manifestation of clinical symptoms such as diarrhoea and rashes

from paratyphoid fever usually occur between 6-30 days after being

serovar paratyphi A, B, or C are estimated to

cause 5.5 million cases of enteric fever each year [4]. In the United

States, paratyphoid fever is uncommon, while, an estimated 5.4 million

outbreaks occur in East Asia in 2000 [5].

Fluoroquinolone derivatives like ciprofloxacin, nalidixic acid etc.

are extensively used to combat paratyphoid fever in most parts of

world. In East Asian countries like India the prevalence of this disease

is too high. In India, newer cephalosporins derivatives, azithromycin,

ciprofloxacin and nalidixic acid [6-8]. The patients with paratyphoid

fever especially in immunocompromised cases have a high rate of

relapse. Due to nature of this phenomena a short course treatment

with either fluoroquinolones or newer cephalosporins derivatives are

ineffective. There are not much data available in this area to provide

satisfactory treatment recommendations. Antibiotics are used to kill

treatment is becoming less effective. Delay in receiving appropriate

antibiotic treatment may have serious consequences that lead to high

rates of mortality [9]. Therefore, some alternative strategies are needed

to treat against strains of

A.

Harold Saton Burr, had performed the detailed studies on the

electrical process happening in body have strong relationship with

magnetic field as described by Ampere's law, which states that the

moving charge produces magnetic fields in surrounding space [11,12].

According to Rivera-Ruiz et al. reported that electrocardiography has

been extensively used to measure the biofield of human body [13].

Thus, human has the ability to harness the energy from environment

or universe and can transmit into any living or nonliving object(s)

Clinical Microbiology: Open AccessTrivedi et al., Clin Microbiol 2015, 4:4

http://dx.doi.org/10.4172/2327-5073.1000215

Research ArticleOpen Access

ISSN:2327-5073 CMO, an open access journalVolume 4 * Issue 4 * 1000215

responding into useful way that is called biofield energy and the

process is known as biofield treatment. Mr. Trivedi's unique biofield

material science [14-16], improved the overall productivity of crops

improved growth and anatomical characteristics of various medicinal

Due to the clinical significance of this organism and literature

reports on biofield treatment as an alternative approach, the present

work was undertaken to evaluate the impact of biofield treatment on

S.

A in relation to antimicrobials susceptibility, minimum

A, American Type Culture Collection (ATCC 9150)

strains were procured from MicroBioLogics, Inc., USA, in two sets A

and B. Two different sealed packs were stored with proper storage

conditions until further use. The antimicrobial susceptibility, MIC

values, biochemical reactions and biotype number were estimated with

the help of MicroScan Walk-Away® (Dade Behring Inc., West

Sacramento, CA, USA) using negative breakpoint combo 30 (NBPC

30) panel with respect to control sample. All the tested antimicrobials

and biochemicals were procured from Sigma-Aldrich (MA, USA).

Two ATCC samples A (revived) and B (lyophilized) of

A were grouped (Gr.). The revived sample A was divided into two

parts Gr.I (control) and Gr.II (revived; treatment); likewise, ATCC B

was labeled as Gr.III (lyophilized; treatment).

Biofield treatment strategy

Gr. I remained as untreated. The treatment Gr. II and III in sealed

pack were handed over to Mr. Trivedi for biofield treatment under

laboratory condition. Mr. Trivedi provided the treatment through his

energy transmission process to the treatment groups without touching

the samples. After treatment, all samples were handed over in the same

condition and stored for analysis. Gr.II was assessed at two time point

i.e. on day 5 and 10 and Gr. III was assessed on day 10. After biofield

treatment, all the groups (control and treated) were investigated for

antimicrobial susceptibility, MIC, biochemical reactions pattern and

biotyping.

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of

carried out with the help of automated instrument, MicroScan Walk-

Away® using NBPC 30 panel. The panel can be stored at 2°C-25°C for

analysis. The panel was allowed to equilibrate to room temperature

prior to rehydration. All opened panel was used within the same day.

The tests carried out on MicroScan were miniaturized of the broth

dilution susceptibility test that has been dehydrated. Briefly, the 0.1 ml

(100 μl) of the standardized suspension of

into 25 ml of inoculum water using pluronic and inverted 8-10 times

and inoculated, rehydrated, and then subjected to incubation for 16

hours at 35°C. Rehydration and inoculation was performed using the

RENOK® system with inoculators-D (B1013-4). 25 ml of standardized

inoculum suspension was poured in to inoculum tray. The detailed

experimental procedures and conditions were followed as per the

manufacturer's instructions. The antimicrobial susceptibility pattern

(S: Susceptible, R: Resistant; and I: Intermediate) and MIC values were

showing inhibition of growth [24].

Biochemical reaction studies

Biochemical activities of

MicroScan Walk-Away®, system with NBPC 30 panel. Preparation of

NBPC 30 panel, inoculum followed by dehydration and rehydration

susceptibility assay for analysis of biochemical reaction followed by

biotype number. The detailed experimental procedures and conditions

were followed as per the manufacturer's instructions [24,25].

Identification of organism by biotype number

MicroScan Walk-Away® processed panel data report with the help of

biochemical reactions data. [24,26].

Antimicrobial susceptibility test

A susceptibility pattern and MIC

values of tested antimicrobials after biofield treatment are summarized

in Tables 1 and 2 respectively. The data were analyzed and compared

with respect to control. Antimicrobial sensitivity was carried out in

twenty antimicrobials. The revived treated cells (Gr. II) of

antimicrobial sensitivity pattern on day 10 while did not show any

sulbactam, aztreonam, chloramphenicol, piperacillin, tetracycline and

ticarcillin/k-clavulanate showed alteration of susceptibility pattern

from S to I in Gr. II on day 10 as compared to control. Moreover,

ciprofloxacin and trimethoprim/sulfamethoxazole showed alteration

of sensitivity pattern from S to R in Gr. II on day 10 as compared to

control after biofield treatment. Eight out of twenty (40%)

antimicrobials did not show any change of antimicrobial sensitivity

after biofield treatment with respect to control sample (Table 1). The

MIC values of amoxicillin/k-clavulanate and ampicillin/sulbactam

were changed from ≤ 8/4 µg/ml to 16/8 µg/ml in Gr. II on day 10.

changed from ≤ 8 µg/ml to >16 µg/ml) in Gr. II on day 10 as compared

to control. Alteration of MIC values (≤ 8 µg/ml to 16 µg/ml) were

aztreonam, cefoxitin, and chloramphenicol except cefuroxime were

changed from ≤ 4 µg/ml to 16 µg/ml on day 10. Moreover, change in

MIC values (≤ 4 µg/ml to 8 µg/ml) were observed in Gr. II in case of

norfloxacin, tetracycline and tobramycin on day 10 after biofield

treatment. Antimicrobials showed an alteration of MIC value (≤ 8

µg/ml to >32 µg/ml) in case of ceftriaxone, (≤ 1 µg/ml to >2 µg/ml) in

case of ciprofloxacin, (≤ 16 µg/ml to 64 µg/ml) in case of piperacillin

and ticarcillin/k-clavulanate, (≤ 2/38 µg/ml to >2/38 µg/ml) in case of

trimethoprim/sulfamethoxazole on day 10 in Gr. II. Besides this,

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Antimicrobial Susceptibility Pattern, Biochemical Characteristics

ISSN:2327-5073 CMO, an open access journalVolume 4 * Issue 4 * 1000215

ESBL-b Scrn showed an alteration of MIC value (≤ 1 to >1 µg/ml) in

Gr. II on day 10. Overall, 53.12% (seventeen out of thirty two) MIC

values were altered after biofield treatment as compared to control.

Fifteen, out of thirty two tested antimicrobials (46.88%) did not show

compared to control (Table 2). Overall, the antimicrobial resistance

pattern (S to I/R) and corresponding MIC values were significantly

altered in revived strain

A after biofield treatment as

compared to control. In this experiment, ciprofloxacin and cefotaxime

were sensitive to control

A (Gr. 1) and the MIC value was

slight increase in ciprofloxacin (≤ 1 µg/ml to >2 µg/ml) while, it did

not alter in case of cefotaxime in Gr.II on day 10. This finding is

supported by literature data [27].

2Ampicillin/sulbactamS S I S

16Piperacillin/tazobactamS S S S

19Ticarcillin/k-clavulanateS S I S

20 Trimethoprim/sulfamethoxazoleS S R S

R: Resistant; I: Intermediate; S: Susceptible; Gr.: Group

: effect of biofield treatment on antimicrobial susceptibility.

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, et al. (2015) Antimicrobial Susceptibility Pattern, Biochemical Characteristics

ISSN:2327-5073 CMO, an open access journalVolume 4 * Issue 4 * 1000215

# data[].attributes.attachment_link.title
Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S.

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	Energy Treatment Impact on the Characteristics of S. Paratyphi A
	

# data[].attributes.subject
	
	
	
	
	Energy Treatment Impact on the Characteristics of S. Paratyphi A
	

# data[].attributes.body
| The current study was attempted to investigate the effect of human energy treatment on the characteristics of Salmonella paratyphi A, in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping.

# data[].attributes.attachment_link.title
"Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S.

# data[].attributes.headline
	
	
	
	
	S. paratyphi – Alternative Strategy for Enteric Fever
	

# data[].attributes.subject
	
	
	
	
	S. paratyphi – Alternative Strategy for Enteric Fever
	

# data[].attributes.body
Read more about an alternative strategy i.e. the unique biofield treatment which could be useful to treat against strains of enteric fever.

# data[].attributes.headline
	
	
	
	
	The Trivedi Effect | Salmonella Paratyphi
	

# data[].attributes.subject
	
	
	
	
	The Trivedi Effect | Salmonella Paratyphi
	

# data[].attributes.body
Read here about the research conducted on S. Paratyphi A. The overall study suggested that the unique human energy treatment had notable impact on S. paratyphi A.

# data[].attributes.headline
	
	
	
	
	Biochemical Characteristics of S. Paratyphi A
	

# data[].attributes.subject
	
	
	
	
	Biochemical Characteristics of S. Paratyphi A
	

# data[].attributes.body
The recent study shows the changes in the biochemical reactions of S.Paratyphi after the unique human energy treatment. Get to know more from the given link.

# data[].attributes.attachment_link.title
Antimicrobial Susceptibility Pattern, Biochemical Characteristics and Biotyping of Salmonella paratyphi A: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi

# data[].attributes.headline
	
	
	
	
	
		Alteration in Antimicrobial Susceptibility Pattern of S. Paratyphi
	

# data[].attributes.subject
	
	
	
	
	
		Alteration in Antimicrobial Susceptibility Pattern of S. Paratyphi
	

# data[].attributes.body
A research paper that shows the effect of unique human energy treatment on antimicrobial susceptibility pattern, biochemical characteristics and biotyping of Salmonella paratyphi A.

# data[].attributes.headline
	
	
	
	
	Effect of Human Energy Treatment on N. otitidis
	

# data[].attributes.subject
	
	
	
	
	Effect of Human Energy Treatment on N. otitidis
	

# data[].attributes.body
Read here about the effect of human energy treatment on N. otitidis. It has a significant impact on Nocardia otitidis.

# data[].attributes.attachment_link.title
Biological Systems: Open Access Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	
		16S rDNA Sequencing of N. otitidis
	

# data[].attributes.subject
	
	
	
	
	
		16S rDNA Sequencing of N. otitidis
	

# data[].attributes.body
The 16S rDNA sequencing of Nocardia otitidis shows that the biofield treatment could be used as an alternate of existing drug therapy in future. Read here for more details.

# data[].attributes.attachment_link.title
Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Academic Room

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	
		Study of N. otitidis Antimicrobials Susceptibility
	

# data[].attributes.subject
	
	
	
	
	
		Study of N. otitidis Antimicrobials Susceptibility
	

# data[].attributes.body
N. otitidis antimicrobials susceptibility analysis shows changes in minimum inhibitory concentration and susceptibility patterns as compared to control sample after biofield energy treatment.

# data[].attributes.attachment_link.readable_text
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Biological Systems: Open Access

Trivedi et al,, Biol Syst Open Access 2015, 4:2

http://dx.doi.org/10.4172/2329-6577.1000143

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Sambhu Charan Mondal2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract.

Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to

pattern, minimum inhibitory concentration (MIC), DNA polymorphism by Random Amplied Polymorphic DNA (RAPD)

and 16S rDNA sequencing. The strain of N. otitidis (ATCC 14630) was divided into two parts, control and treated.

Antimicrobial susceptibility was studied using the broth microdilution technique. Overall, the MIC values of 16.67%

antimicrobials were changed in the treated group of N. otitidis as compared to the control. Moreover, MIC value of

trimethoprim/sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 µg/mL) in the bioeld energy treated

sample as compared to the control without alteration in the sensitivity spectrum. The 16S rDNA analysis showed that

the treated sample was detected as Enterobacter aerogenes strain NCTC10006T (GenBank Accession No: AJ251468)

with 98% identity of gene sequencing data. However, the nearest homolog genus-species was found as Kluyvera

34 to 53% of polymorphism among treated samples as compared to the control. The 16S rDNA sequencing of treated

sample was carried out to correlate the phylogenetic relationship of N. otitidis with other bacterial species. These

results suggested that Mr. Trivedi's bioeld energy treatment has a signicant impact on N. otitidis.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006, E-mail:

Received August 31, 2015; Accepted September 21, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, et al. (2015)

Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Bioeld Treated

Nocardia otitidis. Biol Syst Open Access 4: 143. doi:10.4172/2329-6577.1000143

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

nocardiosis [9] in immunocompromised patients. Infectious Diseases

Society of America (IDSA) reported that between 500 and 1,000 cases

of Nocardia l infections are recognized in the United States each year, of

which 85% are serious pulmonary or systemic infections [10].

Since 1940s, the sulfonamides have been the drugs of choice for

the treatment of nocardiosis [11]. Due to high mortality rate (50%)

in patients with central nervous system (CNS) Nocardia infections

(Nocardia brain abscess) and patients with non-CNS overwhelming or

disseminated disease, the treatment strategy is inadequate. However, the

combination of sulfamethoxazole with trimethoprim is oen used as the

drug of choice for the treatment of nocardiosis [12]. erefore, some

alternative strategies are needed to treat against nocardiosis. Bioeld

energy has been known as an alternative approach which may be useful

as an alternative treatment to Nocardia infected patients. National

Institute of Health/National Center for Complementary and Alternative

Medicine (NIH/NCCAM) have reported that bioeld (putative energy

elds) or electromagnetic based energy therapies were commonly

used to promote the health and healing [13]. Harold Saxton Burr had

performed the detailed studies on the correlation of electric current

with the physiological process and concluded that every single process

in the human body had an electrical signicance [14]. Recently, it was

discovered that all the electrical processes happening in the human

body have strong relationship with the magnetic eld as required by

susceptibility; Bioeld energy treatment; 16S rDNA sequencing;

Random amplied polymorphic DNA

Abbreviations: NIH/NCCAM: National Institute of Health/

National Center for Complementary and Alternative Medicine;

ATCC: American Type Culture Collection; MIC: Minimum Inhibitory

Concentration; OTUs: Operational Taxonomic Units; NCBI: National

Center for Biotechnology Information; MEGA: Molecular Evolutionary

Genetics Analysis; PCR: Polymerase Chain Reaction; RDP: Ribosomal

Database Project; RAPD: Random Amplied Polymorphic DNA; CNS:

e genusNocardiais associated with the group of microorganisms

known as the aerobic actinomycetes and belongs to the family of

dier from the mycobacteria by the possession of short-chain (40 to

60 carbons) mycolic acids [1]. Nocardia otitidis (N. otitidis) is a weak

lamentous Gram-positive, catalase-positive, branching rods shaped

bacterium that appears similar to Actinomyces species. However, it

can usually be dierentiated from Actinomyces by acid-fast staining

[2]. e taxonomic history of the genus Nocardia is controversial [3].

Nocardia typically exhibits varying degrees of acid fastness due to the

presence of cell wall mycolic acid. e genus is typically similar to the

genus of Mycobacterium. Mordarska et al. had studied the short-chain

fatty acids content in the cell wall of Nocardia and Mycobacterium

genera based on gas-liquid chromatography analysis did not nd

any dierence between two genera [4]. Based on immunoblot and

enzyme-linked immunosorbent assay (ELISA) techniques that detect

specic antibodies that appear as common in various Nocardia and

Actinomadura species. ese antigens do not react with the antibodies

produced in response to Mycobacterium tuberculosis infections [5].

Most of Nocardial infections occur in the United States due to inhalation

of airborne spores or mycelial fragments from the environmental

sources [6]. e most common manifestation of Nocardial disease is

pulmonary nocardiosis [7], extrapulmonary disease [8], and ocular

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Mondal SC, et al. (2015) Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

Ampere's law, which states that the moving charge produces magnetic

elds in the surrounding space [15,16]. us, a human body emits the

electromagnetic waves in the form of bio-photons that is also known

as ultra-weak photon emissions (UPE). It surrounds the body and it

is commonly known as bioeld. erefore, the bioeld consists of an

electromagnetic eld, being generated by moving electrically charged

particles (ions, cell, molecule, etc.) inside the human body [17]. e

transfer of information from cell to cell or DNA or storage by biophotons

has been demonstrated in plants, bacteria, animal neutriophil

granulocytes and kidney cells [18]. Prakash et al. in 2015 reported that the

various scientic instruments such as Kirlian photography, polycontrast

interference photography (PIP) and resonance eld imaging (RFI) can

be extensively used to measure the bioeld of human body [19]. us,

human has the ability to harness the energy from the environment or

universe and can transmit into any living or nonliving object(s) around

the Globe. e objects always receive the energy and responding into

the useful way that is called bioeld energy and the process is known as

bioeld energy treatment. Mr. Mahendra Trivedi's unique bioeld energy

treatment (e Trivedi Eect®) has been known to improve the overall

productivity of crops [20,21], altered characteristics features of microbes

[22-24], alter the structural, physical and thermal properties of several

metals [25,26], and improved growth and anatomical characteristics of

various medicinal plants [27,28].

Based on clinical signicance of N. otitidis and signicant impact

of Mr. Trivedi's bioeld energy modality on microbes, the present work

was undertaken to evaluate the impact of Mr. Trivedi's bioeld energy

on N. otitidis in relation to antimicrobials susceptibility, minimum

inhibitory concentration (MIC), random amplied polymorphic DNA

analysis (RAPD) and 16S rDNA sequencing.

N. otitidis, American Type Culture Collection (ATCC 14630) strain

was procured from Bangalore Genei, Bangalore-India in two vials A

and B. Two dierent sealed packs were stored with proper storage

conditions until further use. All the tested antimicrobials were procured

from Sigma-Aldrich, India. e antimicrobial susceptibility and

MIC were estimated with the help of broth micro dilution technique

as per the Clinical Laboratory Standards Institute (CLSI) guidelines

document number M24-A [29]. e 16S rDNA sequencing and DNA

ngerprinting (RAPD) studies were carried out using Ultrapure

Genomic DNA Prep Kit; Cat KT 83 (Bangalore Genei, India).

Experimental design and bioeld treatment strategy

N. otitidis strain was divided into two groups, i.e., control and

treated. e treated group was in sealed pack and handed over to Mr.

Trivedi for bioeld energy treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated group without touching the sample. Aer treatment,

control and treated groups were assessed on day 10 for antimicrobial

susceptibility, and MIC. For RAPD analysis three inoculums (one for

control and the other two for treated named as treated A and B) were

prepared from N. otitidis samples. ese two bioeld treated samples

A and B were sub-cultured by taking 1% inoculum and inoculated to

fresh 5 ml medium and labeled as treatment A-1 and treatment B-1,

respectively. e result of treated sample was compared with respect to

the control. e 16S rDNA analysis was performed on bioeld treated

samples A and its subcultured sample A1.

Antimicrobial susceptibility test

e antimicrobial susceptibility testing is important for clinically

signicant species. e investigation of antimicrobial susceptibility of N.

otitidis was carried out with the help of broth micro dilution technique,

as per CLSI guidelines. Broth micro dilution was recommended

for isolates of rapidly growing mycobacteria (RGM) based on CLSI

published guidelines and recommendations for testing of non-

tuberculous mycobacteria (CLSI, M24-A, 2003) [29]. e detailed

experimental procedure and conditions were followed as per the

manufacturer's instructions. e antimicrobial susceptibility pattern

(S: Susceptible, R: Resistant) and minimum inhibitory concentration

(MIC) were determined by observing the lowest antimicrobial

concentration showing inhibition of growth.

Random amplied polymorphic DNA (RAPD) analysis

For DNA ngerprinting (RAPD) analysis all the treated samples

(A, A1, B, and B1) were incubated at 37°C with 160 rpm for 18 h.

Subsequently, the cultures were spun down, pelleted at 5000 rpm at

40°C for 10 minutes and the genomic DNA was isolated for control

and treated samples using Genomic DNA Prep Kit (Bangalore Genei,

India). RAPD was performed with all samples of N. otitidis using ve

RAPD primers, which were labelled as RBA8A, RBA13A, RBA20A,

RBA10A and RBA15A were adopted from earlier studies. e PCR

mixture contained 2.5 μL each of buer, 4.0 mM each of dNTP, 2.5 μM

each of primer, 5.0 μL (approximately 20 ng) of each genomic DNA,

2U each of Taq polymerase, 1.5 μL of MgCl2 and 9.5 μL of water in

a total of 25 μL with the following PCR amplication protocol; initial

denaturation at 94°C for 7 min, followed by 8 cycles of denaturation at

94°C for 1 min, annealing at 35°C for 1 min, and extension at 72°C for 2

min; and 35 cycle of denaturation at 94°C for 1 min, annealing at 38°C

for 1 min, and extension at 72°C for 1.5 min; and the nal extension

at 72°C for 7 min. Amplied PCR products from all samples (control

and treated) were separated on 1.5% agarose gels at 75 volts, stained

with ethidium bromide and visualized under UV illumination [30]. e

percentage of polymorphism was calculated using following equation:

Percent polymorphism=A/B × 100

Where, A=Number of polymorphic bands in treated sample; and

B=Number of polymorphic bands in control.

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from N. otitidis cells (set A, sample coded

as 9A) using genomic purication kit, according to the manufacturer

instructions. 16S rDNA gene (~ 1.5 kb) fragment was amplied with the

help of high-delity polymerase chain reaction (PCR) using universal

reverse primer (3' -ACGGTCATACCTTGTTACGACTT-5' ). Amplied

products were subjected to gel electrophoresis in 1.0% agarose gel,

stained with ethidium bromide and visualized under UV light in a gel

documentation unit (BioRad Laboratories, USA). e PCR amplied

fragment was puried from the agarose gel using a DNA gel extraction

kit. Sequencing of amplied product was done on a commercial basis

from Bangalore Genei, India. e 16S rDNA sequences obtained were

aligned and compared with the sequences stored in GenBank database

available from National Center for Biotechnology Information (NCBI)

using the algorithm BLASTn program. Multiple sequence alignment/

phylogenetic tree were established using MEGA3.1 molecular soware

Antimicrobial susceptibility test

Antimicrobial susceptibility pattern of Nocardia can vary from

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Mondal SC, et al. (2015) Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of

Volume 4 * Issue 2 * 1000143

ISSN: 2329-6577 BSO, an open access journal

species to species. e therapeutic eectiveness depends on the proper

identication of species in infected patients and on in vitro sensitivity

studies [32]. e antimicrobial sensitivity assay against Nocardia

species had been especially considered in refractory cases. A standard

test for antimicrobials sensitivity assessed by broth micro dilution and

with cation-supplemented Mueller-Hinton broth has been approved by

the National Committee for Clinical Laboratory Standards (NCCLS)

[29]. e outcome of N. otitidis susceptibility pattern and MIC values of

tested antimicrobials aer bioeld energy treatment are summarized in

Table 1 and 2 respectively. e data were analyzed and compared with

respect to the control. Study was carried out in twelve antimicrobials

for assessment of antimicrobial susceptibility and MIC value. e

treated cells of N. otitidis did not show any alteration with respect to

antimicrobial susceptibility pattern as compared to the control (Table

1). e susceptible nature of N. otitidis to amikacin, sulfamethoxazole,

and ciprooxacin and resistance pattern to ceriaxone, amoxicillin-

clavulanic acid, and imipenem were well corroborated with the

literature data [3]. Beside this, the MIC value of trimethoprim/

sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 µg/mL)

in bioeld energy treated sample as compared to the control sample.

Moreover, the MIC value was slightly reduced (>32/16 to 32/16 µg/mL)

in amoxicillin/k-clavulanate aer the bioeld therapy. Overall, 16.67%

out of twelve antimicrobials showed an alteration of MIC values. Rest

of the antimicrobials did not show any alteration of MIC values with

respect to the control sample. Based on the literature, a combination

of sulfa and antimalarial drugs, i.e., trimethoprim/sulfamethoxazole

are the drug of choice against Nocardia infections as compared to the

single sulfa drug [11,12]. In this experiment, the susceptibility nature

of trimethoprim/sulfamethoxazole was constant in both controls as

well as in treated sample, while the MIC value was reduced by two-

fold in treated sample aer Mr. Trivedi's bioeld energy treatment.

Hence, authors assumed that this improvement of MIC value without

alteration of sensitivity pattern may be due to the eect of putative

energy transmit through bioeld healing (Table 2).

Random amplied polymorphic DNA (RAPD) analysis

e treated and control samples were identied on the basis of

their dierent and discriminative RAPD patterns. RAPD is a preferred

tool that is being used now days to correlate the genetic similarity

or mutations between species. e simplicity and wide applicability

of RAPD analysis mainly depend upon the use of short nucleotide

primers, which were unrelated to known DNA sequences of the target

organism [33]. DNA polymorphisms can be eciently detected using

the PCR primers and identify inter-strain variations among species in

treated samples [34]. e degree of relatedness and genetic mapping

can be correlated between similar or dierent treated sample species

e DNA ngerprinting by RAPD analysis using ve primers was

carried out on the control and treated samples. DNA ngerprinting by

RAPD analysis of the control and treated samples are shown in Figure 1,

and the polymorphic bands are marked by arrows. e RAPD patterns

of treated samples showed some unique and dissimilar patterns. DNA

polymorphism analyzed by the RAPD analysis was presented in Table 3.

e level of polymorphism between control and treated samples (A, A1,

B, and B1) are summarized in Table 4. e level of polymorphism was

found in an average range of 34 to 53% in treated samples as compared

to control in N. otitidis aer the bioeld treatment. e highest change

in DNA sequence was observed in treated groups with RBA 13A primer

as compared to the control; a negligible change was found in treated

group with RBA 8A primer as compared to the control (Table 3 and 4).

e bacteria that are poorly dierentiated by conventional methods

needs molecular analysis method like 16S rDNA sequence [36]. is

molecular-based technique is a suitable tool for identication of most

Figure 1: Random amplied polymorphic-DNA fragment patterns of Nocardia

otitidis generated using ve RAPD primers, RBA 8A, RBA 13A, RBA 20A, RBA

10A and RBA 15A. 1: Control; 2: Treated A; 3: Treated A-1; 4: Treated B; 5:

Treated B-1; M: 100 bp DNA Ladder.

11. Amoxicillin/k-clavulanateR R

12. Trimethoprim/sulfamethoxazoleSS

R: Resistant; S: Susceptible; Control: ATCC strain of N. otitidis (without bioeld

energy treatment); Treatment: ATCC strain of N. otitidis (with Mr. Trivedi's bioeld

Table 1: Antimicrobial susceptibility pattern of antimicrobials against ATCC strain of

Nocardia otitidis after bioeld treatment on day 10 as per CLSI guidelines.

2. Clarithromycin>32.0>32.0

10. Gatioxacin 0.12 0.12

11. Amoxicillin/k-clavulanate>32/16 32/16

12. Trimethoprim/sulfamethoxazole0.5/9.50.25/4.75

CLSI: Clinical and Laboratory Standards Institute; MIC values are presented

as µg/mL; Control: ATCC strain of N. otitidis (without bioeld energy treatment);

Treatment: ATCC strain of N. otitidis (with Mr. Trivedi's bioeld energy treatment)

Table 2: Minimum inhibitory concentration (MIC) of antimicrobials against ATCC

strain of Nocardia otitidis after bioeld energy treatment on day 10 as per CLSI

guidelines.

# data[].attributes.attachment_link.title
Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis - PdfSR.com

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	
		Genotyping and Phenotyping Analysis of N. otitidis
	

# data[].attributes.subject
	
	
	
	
	
		Genotyping and Phenotyping Analysis of N. otitidis
	

# data[].attributes.body
Biofield energy treatment, also known as alternative therapy was used in this study to determine the impact on the genotyping & phenotyping analysis of N. otitidis.

# data[].attributes.attachment_link.keywords[]
phylogenetic analysis of nocardia otitidis

# data[].attributes.attachment_link.title
"Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi's biofield energy treatment on N.

# data[].attributes.headline
	
	
	
	
	Biofield Treated N. otitidis| Trivedi Effect
	

# data[].attributes.subject
	
	
	
	
	Biofield Treated N. otitidis| Trivedi Effect
	

# data[].attributes.body
The current study was attempted to investigate the effect of Mr. Trivedi’s biofield energy treatment on N. otitidis for its antimicrobial susceptibility, MIC, and DNA polymorphism.

# data[].attributes.attachment_link.title
Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis

# data[].attributes.attachment_link.description
Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate

# data[].attributes.headline
	
	
	
	
	
		Susceptibility Pattern of Nocardia otitidis: Study of Biofield
	

# data[].attributes.subject
	
	
	
	
	
		Susceptibility Pattern of Nocardia otitidis: Study of Biofield
	

# data[].attributes.body
The analysis of susceptibility pattern of Nocardia otitidis shows alteration in MIC values after the influence of unique energy treatment.

# data[].attributes.headline
	
	
	
	
	Unique Energy Impact on Antibiogram of Nocardia otitidis
	

# data[].attributes.subject
	
	
	
	
	Unique Energy Impact on Antibiogram of Nocardia otitidis
	

# data[].attributes.body
Read here about the impact of biofield treatment on the antibiogram of Nocardia otitidis. The study was conducted to evaluate the impact the biofield energy on various characteristics of Nocardia otitidis.

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	hermal Analysis of Cadmium Powder
	

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	hermal Analysis of Cadmium Powder
	

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In this research, the biofield treatment might have transferred energy to cadmium powder, which stored as potential energy of atoms. Read more information through the link.

# data[].attributes.attachment_link.title
"An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder" by Mahendra Kumar Trivedi

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Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

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	Cadmium Powder | SEM Analysis
	

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	Cadmium Powder | SEM Analysis
	

# data[].attributes.body
The SEM analysis results of Cadmium Powder shows changes in particle sizes that could be due to energy milling through biofield treatment.

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	Cadmium Powder| Trivedi Effect
	

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	Cadmium Powder| Trivedi Effect
	

# data[].attributes.body
Read here about how the Trivedi Effect has affected the unique thermal, physical and structural properties of cadmium powder.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

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		Particle Size and Surface Area Analysis of Cadmium Powder
	

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		Particle Size and Surface Area Analysis of Cadmium Powder
	

# data[].attributes.body
In this experiment, the data showed that the average particle size, d50 was significantly reduced from 70.3 μm (control) to 36.7 μm in treated cadmium powder. Also read for the other magnificent results.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical

# data[].attributes.headline
	
	
	
	
	
		Atomic, Physical and Thermal Properties of Cadmium Powder
	

# data[].attributes.subject
	
	
	
	
	
		Atomic, Physical and Thermal Properties of Cadmium Powder
	

# data[].attributes.body
The DSC, SEM, and XRD analysis data suggest that Mr. Trivedi’s biofield energy treatment has significantly altered the thermal and physical properties of cadmium powder.

# data[].attributes.headline
	
	
	
	
	XRD Analysis of Cadmium Powder
	

# data[].attributes.subject
	
	
	
	
	XRD Analysis of Cadmium Powder
	

# data[].attributes.body
The XRD analysis of cadmium powder showed the change in crystallite size of treated sample as compared to the control, after the influence of human energy treatment.

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	Properties of Cadmium Powder| Biofield Treatment
	

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	Properties of Cadmium Powder| Biofield Treatment
	

# data[].attributes.body
The present study suggests that biofield treatment has significantly altered the atomic, thermal and physical properties of cadmium.

# data[].attributes.attachment_link.title
An Evaluation of Biofield Treatment on Thermal, Physical and Structural Properties of Cadmium Powder - PdfSR.com

# data[].attributes.attachment_link.description
Cadmium is widely utilized in nickel-cadmium batteries, stabilizers, and coating applications due to its versatile physico-chemical properties. The aim of present study was to evaluate the impact of biofield treatment on atomic, thermal, and physical properties of cadmium powder. The cadmium powder was divided into two groups, one group as control and another group as treated.

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		Research on Pseudomonas Fluorescens Antibiotic Susceptibility
	

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		Research on Pseudomonas Fluorescens Antibiotic Susceptibility
	

# data[].attributes.body
Human energy impact was evaluated on various parameters of Pseudomonas Fluorescens, such as antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. Follow the link for more information.

# data[].attributes.attachment_link.keywords[]
antimicrobial sensitivity of p. fluorescens

# data[].attributes.attachment_link.keywords[]
biochemical characteristics of p. fluorescens

# data[].attributes.attachment_link.keywords[]
antimicrobial susceptibility pattern of p. fluorescens

# data[].attributes.attachment_link.title
"Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P.

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	Pseudomonas Fluorescens Characteristics – Human Energy Impact
	

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	Pseudomonas Fluorescens Characteristics – Human Energy Impact
	

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Unique human energy treatment has the ability to alter the characteristics of Pseudomonas Fluorescens. Read here how the human energy could alter the characteristics of Pseudomonas Fluorescens.

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		Change in Pseudomonas Fluorescens Biochemical Reaction Pattern
	

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		Change in Pseudomonas Fluorescens Biochemical Reaction Pattern
	

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Biochemical study of P. Fluorescens samples after the unique spiritual energy treatment revealed significant changes in most of the biochemical reactions. Get more information here.

# data[].attributes.attachment_link.title
Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P.

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		Study of Pseudomonas Fluorescens Biochemical Characteristics
	

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		Study of Pseudomonas Fluorescens Biochemical Characteristics
	

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Study of Pseudomonas Fluorescens Biochemical Characteristics in the presence of unique spiritual energy treatment shows that the energy treatment has considerably altered the susceptibility pattern and MIC value of P. fluorescens.

# data[].attributes.attachment_link.title
Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this

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		Pseudomonas Fluorescens Antibiotic Susceptibility Alterations
	

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		Pseudomonas Fluorescens Antibiotic Susceptibility Alterations
	

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Want to know how energy treatment could change the antibiotic susceptibility of Pseudomonas Fluorescens? Visit here to know about the present research and results.

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	P. Fluorescens Biochemical Tests to Evaluate the Biofield Impact
	

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	P. Fluorescens Biochemical Tests to Evaluate the Biofield Impact
	

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The present study shows the impact of biofield treatment on P. Fluorescens characteristics. P. Fluorescens biochemical tests results revealed a change in biotype number as a results of biofield energy.

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	Spiritual Energy Impact on Pseudomonas Fluorescens Characteristics
	

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	Spiritual Energy Impact on Pseudomonas Fluorescens Characteristics
	

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Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Copyright: © 2015 Trivedi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,

distribution, and reproduction in any medium, provided the original author and source are credited.

Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all

existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment

strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern

of P. fluorescens. P. fluorescens cells were procured from MicroBioLogics in sealed packs bearing the American

Type Culture Collection (ATCC 49838) number. Two sets of ATCC samples were taken in this experiment and

denoted as A and B. ATCC-A sample was revived and divided into two groups (Gr) i.e. Gr.I (control) and Gr.II

(revived); likewise, ATCC-B was labeled as Gr.III (lyophilized). Gr.II and III were given biofield treatment and were

measured by MicroScan Walk-Away® system before and after treatment. Parameters studied in experiment were

antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number of both

control and treatment groups using MicroScan Walk-Away® system. Experimental results showed antimicrobials

showed altered sensitivity and MIC values in treated group as compared to control. Biochemical reactions showed

positive reaction in malonate, melibiose, nitrate, galactosidase, ornithine, raffinose, sorbitol, sucrose, tobramycin and

Voges-Proskauer in Gr.II. Arabinose, colistin, glucose, and rhaminose also showed positive reactions in Gr.II on day

10 while arginine and cetrimide showed negative reaction in Gr.III as compared to control. Biochemical tests results

revealed a change in biotype number in Gr.II (34101173, day 5), (77103177, a very rare biotype on day 10) and

Gr.III (40000043) as compared to control (02041722). Organism was identified as Enterobacter cloacae (GrII, day

10) and Vibrio fluvialis (Gr.III, day 10) with respect to control. These findings suggest that biofield treatment made

significant alteration in sensitivity pattern, MIC values, and biotype number of P. fluorescens.

; Antibiotic susceptibility;

Biofield treatment; Biochemical reactions; Biotyping

obligate aerobic, and considered as a psychrotrophic microorganism,

unable to grow at temperatures above 32°C. Fluorescent species of

pseudomonas, initially considered to be nonpathogenic for human.

Since 1953, human pathogenicity has been reported, when Pittman

describes its presence and characteristics in blood and caused severe to

opportunistic pathogen, and able to inhabit in many environment like

P.

have been reported for high hemolytic activity, which

also resides in the plant's rhizosphere

and produces a variety of secondary metabolites including antibiotics

P.

causing nosocomial infections even higher incidence rate

[3]. Some of these strains found in human digestive

tract with low commensal level while some found in refrigerated food

products with its psychrotrophic characters [4]. Recently, a specific

antigen of was detected in serum of ileal Crohn's disease patient [5].

P.

considered as a pathogenic microorganism. Different antimicrobial

combination therapies are preferred by microbiologist to alter its

sensitivity but due to their associated side effects, alternate approaches

are adopted by healthcare professionals. Among various alternate and

complementary therapies, biofield might be a new approach to do

permeates and surrounds living organisms. It is the scientifically

preferred term for the biologically produced electromagnetic and

functions within the organism. In spite of countless study reports of

the effectiveness of biofield therapies [6], there are very few well

controlled and peer-reviewed experimental studies [7]. According to

law of mass-energy inter-conversion [8], the conversion of mass into

energy is well stabilized, but its inversion i.e. energy into mass is not

yet proved scientifically. Whenever these electrical signals fluctuate

with time, the magnetic field generates as per the Ampere-Maxwell

law, and cumulatively known as electromagnetic field. As responses by

humans can be accounted for by the placebo effect, these experiments

on lower organisms are designed in order to directly test the impact of

biofield energy through scientific studies to rule out the placebo effect.

It is widely accepted that lyophilization is the most preferred method

to store and transport microbial cultures. Therefore, changes in the

biochemical and enzymatic characteristics of an organism cannot be

possible at this storage condition. Mr. Mahendra Trivedi's biofield

Infectious Diseases and TherapyJana et al., J Infect Dis Ther 2015, 3:3

http://dx.doi.org/10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

treatment has been studied significantly and its effects are considerably

microbes against tested antimicrobials [9-11]. However, researchers

found significant inhibitory effect of biofield in different cancer cell

models [12-14]. It has also shown significantly altered the crystalline

and powder characteristics of metals [15,16]. In agriculture, biofield

treated crops have been reported for a significant change on growth,

characteristics and yield of plants [17-19].

There are scanty reports on investigating biofield therapies against

microbes. In the present study, impact of biofield treatment on

P.

was evaluated and its antimicrobial susceptibility pattern,

MIC value, biochemical reactions, and biotype were studied in revived

as compared to control.

ATCC 49838 (American Type Culture Collection)

strains were procured from MicroBioLogics, Inc., USA, in two sets A

and B. The antimicrobials and biochemical used in the study were

procured from Sigma-Aldrich. Two different sealed packs were stored

with proper storage conditions until further use. The antimicrobial

estimated with the help of Microscan Walk-Away system® (Dade

Behring Siemens) using NBPC30 panel with respect to control groups.

Two ATCC samples (ATCC A and B) of

grouped and subjected to biofield treatment. ATCC A sample was

revived and divided into two groups (Gr) viz. Gr.I (control) and Gr.II

(revived); likewise, ATCC B was labeled as Gr.III (lyophilized). Then

group II and III were treated with Mr. Trivedi's biofield energy. Gr.II

was assessed on the 5th and 10th days after treatment while Gr.III was

assessed on 10th day only. Finally, all the groups (control and treated)

reactions pattern and biotyping.

Antimicrobial susceptibility assay

Antimicrobial susceptibility pattern of

using MicroScan Walk-Away® using NBPC30 panel as per

susceptibility pattern (S: Susceptible, I: Intermediate, and R: Resistant)

and minimum inhibitory concentration (MIC) were determined by

observing the change in antimicrobial concentration as per Clinical

and Laboratory Standards Institute (CLSI) guidelines [20].

Biochemical reaction and biotype number studies

organism identification after biofield treatment of

biochemical reactions patterns after biofield treatment were analyzed

using following 33 biochemical such as acetamide, adonitol, arabinose,

arginine, cetrimide, cephalothin, citrate, colistin, esculin, hydrolysis,

nitrofurantoin, glucose, hydrogen sulfide, indole, inositol, kanamycin,

sorbitol, sucrose, tartarate, tryptophan deaminase, tobramycin, urea,

Antimicrobial susceptibility assay

The data pertaining to the antimicrobial susceptibility tests and

details of MIC values were observed and reported in Table 1 and 2

respectively. The result of biofield treatment showed that sensitivity

pattern of ceftriaxone and tetracycline converted from I → R Gr.II on

day 5 and 10. Ceftazidime, ciprofloxacin and piperacillin, converted

from S → R in Gr.II on day 5 and 10 with changes in MIC values as

compared to control. Cefotaxime sensitivity pattern changed from I →

R in Gr.II while in cefepime, changed from I → R only at day 10 in

Gr.II. Tobramycin sensitivity pattern after treatment converted from S

→ I with altered MIC value on day 5 while S → R on day 10 in Gr.II. In

case of norfloxacin, change in MIC value was reported greater than 8

µg/mL in Gr.II as compared with control. Rest of tested antimicrobials

did not showed any change in susceptibility pattern and MIC values

after biofield treatment as compared to control (Table 2).

2Amoxicillin/K-clavulanate- - - -

3Ampicillin/Sulbactam - - - -

16Piperacillin/TazobactamS S S S

19Ticarcillin/K-ClavulanateR R R R

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

Treatment. J Infect Dis Ther 3: 222. doi:10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

R: Resistant; I: Intermediate; S: Susceptible; '-': Not reported; Gr: group

Table 1: Effect of biofield treatment on antimicrobial susceptibility of

S.

2Amoxicillin/K-clavulanate>16/8 >16/8 >16/8 >16/8

3Ampicillin/Sulbactam>16/8 >16/8 >16/8 >16/8

5Aztreonam>16 >16 >16 >16

6Cefazolin>16 >16 >16 >16

10Cefoxitin>16 >16 >16 >16

14Cephalothin>16 >16 >16 >16

15Chloramphenicol>16 >16 >16 >16

16Ciprofloxacin≤1 >2 >2 ≤1

23Nitrofurantoin>64 >64 >64 >64

28 Ticarcillin/K-Clavulanate>64>64 >64 >64

Sulfamethoxazole >2/38 >2/38 >2/38 >2/38

MIC values are presented in µg/mL; Gr: Group

Table 2: Minimum inhibitory concentration (MIC) of

for tested antimicrobials

Biochemical tests denoted with codes are summarized in Table 3.

Results showed positive reactions (i.e. (-) negative to (+) positive) in

case of malonate, melibiose, nitrate, galactosidase, ornithine, raffinose,

sorbitol, sucrose, tobramycin, and Voges-Proskauer on day 5 and 10 in

Gr II on both days with respect to control. Arabinose, colistin, and

rhaminose showed negative reactions (i.e. (+) positive to (-) negative)

converted into positive to negative reaction in Gr.III and in Gr.II at

day 5 only. Cetrimide showed negative reaction in Gr.III as compared

to control. Glucose showed positive reaction at day 10 in Gr.II and

Gr.III as compared to control.

S.

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield

Treatment. J Infect Dis Ther 3: 222. doi:10.4172/2332-0877.1000222

ISSN:2332-0877 JIDT, an open access journalVolume 3 * Issue 3 * 1000222

# data[].attributes.attachment_link.title
Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P.

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		Atomic Properties of Carbon Allotrope Powders
	

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		Atomic Properties of Carbon Allotrope Powders
	

# data[].attributes.body
Influence of unique consciousness energy shows the change in atomic parameters which results in significant change in crystallite & particle sizes of treated sample of Carbon Allotrope Powders.

# data[].attributes.attachment_link.title
"Effect of superconsciousness external energy on the atomic, crystalline and powder characterisitics of "carbon allotrope powders"" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding energy and matter always co- existed and is considered one and the same.

# data[].attributes.headline
	
	
	
	
	
		Carbon Allotrope Particle Size Analysis
	

# data[].attributes.subject
	
	
	
	
	
		Carbon Allotrope Particle Size Analysis
	

# data[].attributes.body
The particle size of different Carbon Allotropes was evaluated in this research work to investigate the impact of a unique consciousness energy.

# data[].attributes.headline
	
	
	
	
	 Alteration in Carbon Allotrope Powders Properties
	

# data[].attributes.subject
	
	
	
	
	 Alteration in Carbon Allotrope Powders Properties
	

# data[].attributes.body
Due to the influence of unique consciousness energy, there was the alteration in Carbon Allotropes' properties i.e. physical and structural properties.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Effect of superconsciousness external energy

on atomic, crystalline and powder

characteristics of carbon allotrope powders

Scientists are searching for eluding link between spirituality and science. Some believe

fundamental essences of universe to be energy and information. As per current understanding,

energy and matter always coexisted and is considered one and the same. Energy is considered

as 'matter in perpetual motion' and matter as 'stationary energy'. Interconversion between matter

and energy has been defined by Einstein's famous energy-mass equation (E5mc

been proven by nuclear physicists using complex nuclear reactions involving high energy

particles. However, many spiritual masters have claimed to realise this energy-matter

interconversion using their spiritual powers/energy but scientifically unknown and unverified. It

is the first time that the lead author (M. K. Trivedi) has been using his unique superconsciousness

energy in the form of thought intervention and information signals to bring about dramatic and

radical transformations in the physical and structural properties of organic and inorganic

materials. The present paper is the first scientific report that deals with the effect of consciousness

energy which M. K. Trivedi uniquely communicates through thought intervention by sending an

information signal that transforms carbon allotropes. The changes the energy has caused at the

atomic, molecular and crystalline levels in diamond, graphite and activated charcoal have been

studied very systematically and are reported in this paper. It has been observed that the

superconsciousness energy when transmitted to carbon allotropes has changed the lattice

parameters of unit cells, crystallite sizes and densities. Computed weight and effective nuclear

charge of the treated atoms exhibited significant variation. It is believed that the energy is acting

on the nuclei causing their transmutation.

Keywords: Consciousness energy, Thought intervention, Diamond, Graphite, Activated charcoal, X-ray diffraction, Particle size, Atomic weight, Atomic

Scienceattempts to preserve its value free nature so as to

retain validity and avoid interference with religious

bodies and beliefs. If rituals and dogmas are removed

from religion, the result is spirituality that is consistent

with science and can be related to it. Margenau

stated that the existence of such a relationship is evident

from the philosophical preoccupations and writings of

the most distinguished and creative physicists of the last

ﬁve decades, such as Einstein, Bohr, Heisenberg,

Schro¨dinger, Dirac, Wigner and many others. While

answering the question as to what precisely constitutes a

miracle, he had suggested that science, being not yet and

quite possibly will never be a complete system of

explanation. For example, the existence of man as well

as of the entire universe has long been regarded as

miracle, incomprehensible without assuming the exis-

tence of a 'directed creative force' which is omnipotent

and omniscient. During this century, the Big Bang

Theory was formulated and conﬁrmed. A very small but

extremely massive sphere of matter, in many respects

similar to a black hole, could apparently spring out of

nothing without violating any known law of nature.

quoted Schleiermacher's words 'the exis-

tenceof thelawsof nature is the greatestofall miracles'.

Matter, energy and information signals

There are several attempts to develop a model linking

the macroscopic universe and the microscopic atomic

and subatomic world. The electromagnetic spectrum of

which the visible light forms a small portion arises

from the cosmic radiation ﬁltered by the atmosphere

DivineLife Foundation, Gurnee,IL,USA

Universityof SouthernCalifornia,Los Angeles, CA, USA

OklahomaState Universit y, Stillwater, OK, USA

ßW.S. Maney & Son Ltd. 2009

Received9 July 2009; accepted 4 August 2009

DOI10.1179/143289109X12494867167602MaterialsResearch Innovations2009 VOL 13 NO 4473

surrounding the planet. The observed properties of the

matter as well as the electromagnetic radiation could

onlybe explained by assuming a dual nature. That is, the

object can be viewed as a particle, and words such as

mass, speed and energy are used to describe it. On the

other hand, the object can be viewed as a wave and

described by words like wavelength and frequency. This

dual nature of matter and radiation led to quantum

indeterminacy. The apparent and necessary incomplete-

ness in the description of a physical system has thus

become one of the characteristics of the nature of

quantum physics. Before quantum physics, itwas

(i) astate of a physical system can be uniquely

determined by all the values of its measurable

properties, and conversely

(ii)the values of the measurable properties uniquely

This made Albert Einstein sceptical of the quantum

indeterminacy and in a 1926 letter to Max Born,

wrote: 'I, at any rate, am convinced that He

[God] does not throw dice' which became the most

famous philosophical statement.

The atoms and subatomic particles are in continuous

movement. Einstein postulated that neither thetime nor

the weight or mass are constant. When moving at high

speeds,all ofthesethings get compressed;onlythe speed

of light remains the same. That happens because,

energy is equal to mass times

the speed of light squared, or E5mc

This hadled to the concept of the atomic bomb where

the mass of the tiny atom is converted to tremendous

energy. The reverse, which is conversion of energy to

mass, is yet to be observed.

Gravity is yet another energy that plays a dominant

role at the macroscopic level (at the level of large masses

such as human beings, planets, etc). Einstein attempted

to generalise his theory of gravitation in order to ﬁnd a

single unifying force the special cases of which are

gravitation and electromagnetism. Since then, the

theoretical physicists are in search of an elusive uniﬁed

ﬁeld theory (single fundamental form of energy that

encompasses all forms of forces) that may combine the

four known fundamental forces, namely, strong nuclear

interaction, electromagnetic interaction, weak nuclear

interaction and gravitational interaction. However, all

attempts to identify such super energy so far have failed.

'The trigger' in 1999, where they considered the well

known CERN model to be incomplete as it did not say

anything about why the matter existed. Rather, the

understanding is that energy can be energy, as well as

matter. Forces are assumed to be transmitted by vector

bosons, which are considered to be elementary particles,

and the confusion is further compounded because

particles are matter, and matter is energy.

hypothesised that information not

only organises and differentiates energy but also

regularises and stabilises matter. Information is con-

sidered to bea mindofcreationandpropagates through

matter-energy mediating in their interactions. Matter is

assumed to be a mere derivation, a subordinate

phenomenon of the fundamental essences of energy

and information. They proposed that information binds

energy into form just as will binds volition into purpose.

As a consequence, the form can be altered by altering

the information without changing the substance. Thus,

the information is considered to be the Universe's will,

imposing order on the Universe's substance, an order

which has followed in the wake of the spontaneous and

explosive transformation wrongly taken as the moment

of creation. Energy is considered to be older than

information, but formless and timeless without it, older

than matter, but helpless and useless without it. In their

theBigBangisnot the birth

of the Universe, but the birth of its consciousness.

Unknown energy causes transformation

Many spiritual masters have claimed to realise the

energy-matter interconversion using their spiritual

powers/energy but scientiﬁcally unknown and unveri-

ﬁed. It is the ﬁrst time that the lead author (M. K.

Trivedi) has this unique ability that he can use his

unique superconsciousness energy in the form of

thought intervention and information signals to bring

about dramatic and radical transformations in the

physical and structural properties of organic and

He deﬁes the laws of human physiology and has been

performingwhat a laymanwouldcall 'miracles'.Hehas,

within last12 years,curedbyphysicaltouch/thought

intervention thousands of people in several countries all

around the world suffering from physical, mental,

spiritual and emotional disorders. For the ﬁrst time,

scientiﬁc experiments on inorganic and organic materi-

als using standard scientiﬁc procedures. The results on

specialclassof material, i.e. carbon allotropes (activated

carbon, graphite and diamond), are reported hereunder

Activated carbon (Merck), graphite (Alpha Aesar) and

natural diamond monocrystalline powders (Sigma

Aldrich) are selected for the investigation. One portion

of these is kept as a control/standard sample, while the

other four portions are exposed to different quanta of

M. K. Trivedi's superconsciousness energy on different

occasions and are referred to as T1, T2, T3 and T4

(treated) samples. In order to eliminate the unforeseen

errors, only normalised parameters are used for com-

parison. The superconsciousness energy was transmitted

to this material through thought intervention.

Average particle size and size distribution are

determined by Sympatec HELOS-BF laser particle size

particle size distribution, d

(the average particle size)

(maximum particle size below which 99% of

particles are present) for the control (untreated or as

received powders) are taken as standard and are

compared with the results obtained on four separately

Both the control and treated samples were analysed

by X-ray diffraction using PW 1710 XRD system

(Phillips,Eindhoven, The Netherlands). A copper anode

withnickel ﬁlter is used. Thewavelengthof the radiation

Trivedi and TallapragadaSuperconsciousness energy and carbon allotropes

Particle size and size distribution

various powders. Per cent change in particle size of

treated powders with respect to control powders is

computed using the formula

is the change in average particle size.

Ina similar manner, the change in particlesized

is computed. These values can be seen in Tables 1 and 2

and are plotted in Fig. 1 (the diamond samples are too

small and hence, particle size and surface area could not

in graphite showed a decrease up to 22?2%,

reverse happened in the case of d

44? 6% in activated charcoal, while it increased up to

possibly due to deformation parallel to c axis. The

smaller particles as indicated by d

fractured at the interparticle and agglomerate bound-

aries, thereby reducing the d

size. These results suggest

that the particles may have elongated, workhardened

and eventually fractured to smaller particles, indicating

that the external energy has acted at the polycrystalline

level causing deformation and fracture of particles as if

the particles had undergone a high energy milling.

What must be happening to cause these signiﬁcant

changes in particle size? In order to ﬁnd a probable

cause, the powders are examined by X-ray diffraction.

X-ray diffraction patterns of control samples of

samples are at 2h 543? 89, 75? 29 and 91? 5 u . The ﬁrst two

are due to (111) and (220) planes, while the third peak

can be due to rhombohedral diamond (1034). The

intensities of the peaks have nearly remained same. As

compared to JCPDS spectrum, both the control and

treated samples showed additional weak pattern corre-

sponding to rhombohedral form of carbon (JCPDS 79-

Graphite X-ray diffraction patterns show peaks

representing the hexagonal (002) basal plane at 2h 5

26?54u and (004) plane at 54 ?66u respectively. The peak

Three broad peaks are observed in activated charcoal

at 2h of around 26? 5, 31? 3 and 44? 6u in the order of

decreasing intensity. The ﬁrst peak can be due to

graphitised fraction. Furthermore, the weaker broad

peak at 43u indicates that the (100) and (101) peaks have

of control and treated powders

TreatmentAverage particle size

of control and treated powders

TreatmentAverage particle size

Trivediand TallapragadaSuper consciousness energy and carbon allotropes

# data[].attributes.attachment_link.title
Effect of superconsciousness external energy on atomic, crystalline and powder characteristics of carbon allotrope powders - PdfSR.com

# data[].attributes.attachment_link.description
Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding, energy and matter always coexisted and is considered one and the same. Energy is considered as 'matter in perpetual motion' and matter as 'stationary energy'.

# data[].attributes.headline
	
	
	
	
	Deformation in Carbon Allotrope Powders
	

# data[].attributes.subject
	
	
	
	
	Deformation in Carbon Allotrope Powders
	

# data[].attributes.body
The deformation in Carbon Allotrope powders was evaluated in this research paper which is due to the impact of energy healing therapy from Mr. Mahendra Trivedi.

# data[].attributes.attachment_link.title
Effect of superconsciousness external energy on atomic, crystalline and powder characteristics of carbon allotrope powders

# data[].attributes.attachment_link.description
Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding, energy and matter always coexisted and is considered one and the

# data[].attributes.headline
	
	
	
	
	
		Characteristics of Carbon Allotrope Powders
	

# data[].attributes.subject
	
	
	
	
	
		Characteristics of Carbon Allotrope Powders
	

# data[].attributes.body
The present paper illustrates the effect of consciousness-energy which M. K. Trivedi uniquely communicates through thought intervention by sending an information signal that transforms the Carbon Allotropes.

# data[].attributes.attachment_link.title
Effect of superconsciousness external energy on the atomic, crystalline and powder characterisitics of "carbon allotrope powders"

# data[].attributes.attachment_link.description
Scientists are searching for eluding link between spirituality and science. Some believe fundamental essences of universe to be energy and information. As per current understanding, energy and matter always coexisted and is considered one and the same. Energy is considered as 'matter in perpetual motion' and matter as 'stationary energy'.

# data[].attributes.headline
	
	
	
	
	
		Carbon Allotropes - XRD Analysis
	

# data[].attributes.subject
	
	
	
	
	
		Carbon Allotropes - XRD Analysis
	

# data[].attributes.body
The XRD analysis of Carbon Allotrope powders has reported the change in characteristics of tested powders due to the use of Mr. Trivedi's consciousness energy.

# data[].attributes.headline
	
	
	
	
	
		Analysis of Deformation in Carbon Allotropes
	

# data[].attributes.subject
	
	
	
	
	
		Analysis of Deformation in Carbon Allotropes
	

# data[].attributes.body
The study was done to evaluate the effect of Mr. Trivedi's consciousness-energy which is reported to alter the characteristics of Carbon Allotropes in the present research work.

# data[].attributes.headline
	
	
	
	
	
		 HPLC Chromatogram of Benzophenone
	

# data[].attributes.subject
	
	
	
	
	
		 HPLC Chromatogram of Benzophenone
	

# data[].attributes.body
Visit this link to get to know about the changes that the unique spiritual energy treatment has brought to the Benzophenone sample and evaluated using HPLC Chromatogram of Benzophenone.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Published online November 9, 2015 (http://www.sciencepublishinggroup.com/j/sjac)

doi: 10.11648/j.sjac.20150306.15

ISSN: 2376-8045 (Print); ISSN: 2376-8053 (Online)

Thermal, Spectroscopic and Chromatographic

Characterization of Biofield Energy Treated Benzophenone

Trivedi Global Inc., Henderson, NV, USA

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana. Thermal, Spectroscopic and

Chromatographic Characterization of Biofield Energy Treated Benzophenone. Science Journal of Analytical Chemistry.

Vol. 3, No. 6, 2015, pp. 109-114. doi: 10.11648/j.sjac.20150306.15

The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic,

and chemical properties of benzophenone. The study was doneusing various analytical methods such as gas

calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) spectroscopy. The

benzophenone sample was divided into two parts, one part was subjected to Mr. Trivedi's biofield energy treatment, called as

treated and the other part was remained as untreated, called as control. Mass spectra showed the molecular ion peak at m/z = 182

in control and all the treated benzophenone samples with different intensities (treated samples further divided in to three parts, T1,

T2, and T3 for GC-MS study). The isotopic abundance ratio of

C (PM+1)/PM and in treated sample was decreased

by 44.87% in T2 and slightly increased upto 5.79% in case of T1 as compared to the control [where, PM- primary molecule,

(PM+1)- isotopic molecule either for

H]. Moreover, isotopic abundance ratio of

O (PM+2)/PM in the treated sample

was increased up to 22.64% in T3. The retention time of treated benzophenone was slightly increased (0.88 min) as compared to

the control in HPLC chromatogram. The DSC data exhibited that the heat of degradation of treated benzophenone was increased

by 674.16% as compared to the control. While, C=O stretching frequency of treated sample was shifted by 6 cm

region in FT-IR spectroscopy. Further, the UV-Vis spectra of control sample showed characteristic ab sorption peaks at 210 nm

and 257 nm that was blue shifted to 205 nm and 252 nm, respectively in the treated sample. These results suggested that biofield

treatment has significantly altered the thermal, spectroscopic, and chemical properties of benzophenone, which could make them

more useful as reaction intermediate in industrial applications.

Biofield Energy Treatment, Benzophenone, Gas Chromatography-Mass Spectrometry,

High Performance Liquid Chromatography

Benzophenone is an a romatic ketone, generally used in

cosmetic products as ultr aviolet (UV) absorber however it is

suspected of causing cancer and toxic to aquatic li fe with

long-term effects [1]. The UV absorption arises from the

conjugation of the electrons between the two benzene rings

and the carbonyl C=O group (Figure 1) [2].

The chemicals that absorb UV rad iation in the range of

280-400 nm are called UV filter molecules. These chemicals

are added to sunscreen products such as lipsticks, lotions,

concentration upto 10% [3]. Apart from their advantageous

reproduction and development [4]. Moreover, benzophenone

can be used as a photo initiator in UV-curing applications [5],

content. The use of benzophenone allows manufacturers to

package the products in clear glass or plastic without which,

opaque or dark packaging would be required [6]. Further, the

insecticidal activities of benzophenone hydrazones were first

described by researchers at DuPont in 1973. Subsequently,

110 Mahendra Kumar Trivedi, et al.: Thermal, Spectroscopic and Chromatographic

Characterization of Biofield Energy Treated Benzophenone

insecticidal activity against lepidopteron and other insect

pests [7]. B enzophenone is used as flavor ingredient, a

fragrance enhancer, a perfume fixative, and a n additive for

plastics coating and adhesive formulations. It is also used in

hypnotic drugs, antihistamines and other pharmaceuticals [8].

Figure 1. Structure of benzophenone.

contributes to carcinogenicity due to the high reactivity and

instability of benzophenone anion (benzophenone work via

formation of an anion). Hence, the stability of benzophenone is

important to perform controlled reduction at normal reaction

conditions, which could be enhanced by Mr. Trivedi's unique

biofield energy treatment, which is already known to alter the

physical and structural properties of various living organisms

and non-living substances [9]. The biofield is the energy fields

that purportedly surround and penetrate the human body.

Human has the ability to harness this form of energy from the

universe and can transmit into any object around the Globe.

The object(s) always receive the energy and responded into

useful way. This process is known as biofield treatment. Mr.

Trivedi's unique biofield treatment is also called as The Trivedi

. The impact of the Trivedi Effect has been well studied

in various research fields like microbiology [9, 10], materials

sciences [11, 12], and agricultural research [13]. Based on the

microbiology, materials sciences, and in agricultural research,

treatment on thermal, spectroscopic and chemical properties

with special interest of change in isotopic abundance ratio,

(PM+1)/PM and (PM+2)/PM in benzophenone.

Benzophenone was procured from Research Lab., India.

The compound was distrib uted into two groups, where one

part was referred as control and the other as treated group. The

treated group in sealed pack was handed over to Mr. Trivedi

for biofield treatment through his energy transmission process

under standard laboratory conditions. The control and treated

samples were characterized using, gas chromatography-mass

chromatography (HPLC), differential scanning calorimetry

(DSC), Fourier transform infrared (FT-IR) spectroscopy, and

ultraviolet-visible (UV-Vis) spectroscopy.

analysis was performed on Perkin Elmer/auto system XL with

Turbo mass, USA, having detection limit up to 1 picogram.

For GC-MS anal ysis the treat ed sample was further divide d

into three parts as T1, T2, and T3. The GC-MS spectra were

plotted as the % abundance vs. mass to charge ratio (m/z). The

O, (PM+2)/PM was expressed by its deviation in treated

samples as compared to the control. The percentage change in

isotopic ratio (PM+1)/PM and (PM+2)/PM were calculated on

a percentage scale from the following formula:

Percentchangeinisotopicabundanceratio

are the ratio of intensity at

(PM+1) to PM in mass spectra of treated and control samples,

The HPLC analysis was performed on a Knauer High

equipped with Smartline Pump 1000 and a UV 2600 detector.

Chromatographic separation was performed on a C

(Eurospher 100) with a dimension of 250 × 4 mm and 5 µm

particle size. The mobile phase used was methanol with a flow

rate of 1 mL/min. Temperature of the experiment was kept

~25°C. The solutions of standard and the samples were

prepared in methanol. The method development for this assay

solvent methanol was used as the diluent.

Elmer/Pyris-1, US A, with a heating rate of 10°C/min and

nitrogen flow of 5 mL/min was used for thermal analysis. The

change in latent heat (∆H) of control and treated ethanol was

recorded from their respective DSC curves.

The FT-IR spectra were obtained from Shimadzu's Fourier

transform infrared spectrometer (Japan) in the frequency

. FT-IR of control and treated samples

of benzophenone was carried out to determine the effect of

biofield energy treatment on molecular level like dipole

moment, force constant, and bond strength in the compound.

crystals with spectroscopic grade KBr into fine powder and

then pressed into pellets.

Science Journal of Analytical Chemistry 2015; 3(6): 109-114 111

UV spectra of control and treated samples of benzophenone

spectrophotometer. Quartz cell with 1 cm and a slit width of

2.0 nm were used for analysis within the wavelength region of

3. Results and Discussion

The GC-MS spectra of control, and treated samples of

benzophenone are presented in Figure 2. Mass spectra showed

intensities. However, the base peak was found at m/z = 105 in

control, T1 and T3 samples. The intensity ratio of (PM+1)/PM

and (PM+2)/PM are presented in Table 1 and percent change

in isotopic abundance ratio was calculated and shown in

Figure 3. Four major peaks at m/z= 182, 105, 77, and 51 were

observed in both control and treated samples of benzophenone

due to the following ions respectively: C

ions. Peaks at m/z=105, 77, and 51 were

benzaldehyde, phenyl and buten-3-yne ions. All peaks were

same for both control and treated samples and well matched

with reported literature [14].

benzophenone sample was slightly increased in T1 (5.79%)

and T3 (4.47%) and decreased significantly in T2 (-44.87%)

abundance ratio of (PM+2)/PM was increased significantly up

to 22.64% in T3 (T1=20.84%, T2= 6.4 %,). The decreased

benzophenone may decrease µ (effective mass) and binding

energy in this molecule with lighter isotopes, where the

increased isotope abundance ratio of (PM+2)/PM in treated

benzophenone may increase µ (effective mass) and binding

energy of C-O bond. This may alter the property of the

chemical bond and e ventually property of the molecule. The

transformation may be happened in the nuclear level due to the

biofield treatment. It is expected that some of

strength also plays an impo rtant role in kinetic e ffects due to

the greater strength of the isotopic bonds such as

Table 1. GC-MS isotopic abundance analysis result of benzophenone.

Peak Intensity Control Treated

Figure 2. GC-MS spectra of control and treated benzophenone sample.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia, Snehasis Jana

# data[].attributes.attachment_link.title
Thermal, Spectroscopic and Chromatographic Characterization of Biofield Energy Treated Benzophenone - PdfSR.com

# data[].attributes.attachment_link.description
The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic, and chemical properties of benzophenone. The study was done using various analytical methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) spectroscopy.

# data[].attributes.headline
	
	
	
	
	GC-MS Analysis of Benzophenone
	

# data[].attributes.subject
	
	
	
	
	GC-MS Analysis of Benzophenone
	

# data[].attributes.body
The GC-MS study of Benzophenone shows the alteration in the isotopic abundance ratio in the treated sample as compared to the control, which was due to the effect of unique energy treatment.

# data[].attributes.attachment_link.title
"Thermal, Spectroscopic and Chromatographic Characterization of Biofield Energy Treated Benzophenone" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic, and chemical properties of benzophenone. The study was done using various analytical methods such as gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), differential scanning calorimetry (DSC), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) spectroscopy.

# data[].attributes.headline
	
	
	
	
	DSC Analysis of Benzophenone
	

# data[].attributes.subject
	
	
	
	
	DSC Analysis of Benzophenone
	

# data[].attributes.body
DSC analysis of Benzophenone was used for the thermal analysis after its treatment with the unique energy therapy by Mr Mahendra Trivedi.

# data[].attributes.headline
	
	
	
	
	
		Thermal & Spectroscopic Properties of Benzophenone
	

# data[].attributes.subject
	
	
	
	
	
		Thermal & Spectroscopic Properties of Benzophenone
	

# data[].attributes.body
Read here the impact of biofield energy treatment on the thermal, chemical and spectroscopic properties of Benzophenone.

# data[].attributes.headline
	
	
	
	
	
		 Assessment of Benzophenone UV Spectrum
	

# data[].attributes.subject
	
	
	
	
	
		 Assessment of Benzophenone UV Spectrum
	

# data[].attributes.body
The aim of this study was to determine the changes in spectroscopic properties of Benzophenone using the UV spectrum of both treated and the control sample. The changes were due to the spiritual energy treatment of the sample.

# data[].attributes.headline
	
	
	
	
	Benzophenone IR Spectrum Analysis
	

# data[].attributes.subject
	
	
	
	
	Benzophenone IR Spectrum Analysis
	

# data[].attributes.body
Benzophenone IR spectrum analysis shows the shifting in C=O stretching frequency of the alternative energy treated sample as compared to the control.

# data[].attributes.attachment_link.title
Thermal, Spectroscopic and Chromatographic Characterization of Biofield Energy Treated Benzophenone

# data[].attributes.attachment_link.description
The aim of the present study was to evaluate the impact of biofield energy treatment on the thermal, spectroscopic, and chemical properties of benzophenone. The study was done using various analytical methods such as gas chromatography-mass

# data[].attributes.headline
	
	
	
	
	
		Thermal Characterization of Benzophenone
	

# data[].attributes.subject
	
	
	
	
	
		Thermal Characterization of Benzophenone
	

# data[].attributes.body
The current study was designed to evaluate the impact of human energy treatment on the spectroscopic and thermal characterization of Benzophenone.

# data[].attributes.headline
	
	
	
	
	
		Analysis of p-Chlorobenzophenone
	

# data[].attributes.subject
	
	
	
	
	
		Analysis of p-Chlorobenzophenone
	

# data[].attributes.body
The XRD, particle size and thermal analysis data suggest that the alternative energy treatment has a significant impact on the physical and thermal properties of treated p-Chlorobenzophenone as compared to the untreated sample.

# data[].attributes.headline
	
	
	
	
	 Physicochemical Characteristics of p-Chlorobenzophenone
	

# data[].attributes.subject
	
	
	
	
	 Physicochemical Characteristics of p-Chlorobenzophenone
	

# data[].attributes.body
The current study was undertaken to evaluate the impact of unique energy treatment on physicochemical and spectroscopic characteristics of p-Chlorobenzophenone.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, Snehasis Jana

# data[].attributes.attachment_link.title
Physicochemical and Spectroscopic Characteristics of Biofield Treated p-Chlorobenzophenone - PdfSR.com

# data[].attributes.attachment_link.description
p-Chlorobenzophenone (p-CBP) is the important chemical intermediate used for the synthesis of several pharmaceutical drugs like fenofibrate, cetirizine, alprazolam, and benzodiazepine. The aim of this study was set to evaluate the impact of biofield energy treatment on physicochemical and spectroscopic properties of p-CBP. The study was accomplished in two groups i.e.

# data[].attributes.headline
	
	
	
	
	
		Tghermal Properties of p-Chlorobenzophenone
	

# data[].attributes.subject
	
	
	
	
	
		Tghermal Properties of p-Chlorobenzophenone
	

# data[].attributes.body
The study suggests that due to the impact of human energy treatment the thermal properties of p-Chlorobenzophenone have been altered significantly. Read here for the more details.

# data[].attributes.attachment_link.title
"Physicochemical and Spectroscopic Characteristics of Biofield Treated p-Chlorobenzophenone" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
p-Chlorobenzophenone (p-CBP) is the important chemical intermediate used for the synthesis of several pharmaceutical drugs like fenofibrate, cetirizine, alprazolam, and benzodiazepine. The aim of this study was set to evaluate the impact of biofield energy treatment on physicochemical and spectroscopic properties of p-CBP. The study was accomplished in two groups i.e.

# data[].attributes.headline
	
	
	
	
	
		 P-Chlorobenzophenone' FTIR Analysis
	

# data[].attributes.subject
	
	
	
	
	
		 P-Chlorobenzophenone' FTIR Analysis
	

# data[].attributes.body
In this study, p-Chlorobenzophenone is tested for the change in Physicochemical and Spectroscopic properties due to the human energy treatment. P-Chlorobenzophenone' FTIR Analysis is used to identify the Spectroscopic characteristics of P-Chlorobenzophenone.

# data[].attributes.headline
	
	
	
	
	XRD Analysis of p-Chlorobenzophenone
	

# data[].attributes.subject
	
	
	
	
	XRD Analysis of p-Chlorobenzophenone
	

# data[].attributes.body
The XRD analysis of P-Chlorobenzophenone shows significant changes in crystallite size of the sample that has been treated with the unique human energy treatment.

# data[].attributes.headline
	
	
	
	
	Analysis of Physical Properties of p-CBP
	

# data[].attributes.subject
	
	
	
	
	Analysis of Physical Properties of p-CBP
	

# data[].attributes.body
The influence of unique energy treatment was observed on the physical properties of p-CBP in a research study. Follow the link to read the full research paper.

# data[].attributes.attachment_link.description
p-Chlorobenzophenone (p-CBP) is the important chemical intermediate used for the synthesis of several pharmaceutical drugs like fenofibrate, cetirizine, alprazolam, and benzodiazepine. The aim of this study was set to evaluate the impact of biofield

# data[].attributes.headline
	
	
	
	
	
		 Assessment of p-Chlorobenzophenone Characteristics
	

# data[].attributes.subject
	
	
	
	
	
		 Assessment of p-Chlorobenzophenone Characteristics
	

# data[].attributes.body
The main aim of this study was to evaluate the impact of energy treatment on p-Chlorobenzophenone' characteristics. For more details visit the link.

# data[].attributes.headline
	
	
	
	
	
		 C. braakii - Biotype Number Analysis
	

# data[].attributes.subject
	
	
	
	
	
		 C. braakii - Biotype Number Analysis
	

# data[].attributes.body
The unique energy treatment has significant impact on the antimicrobial susceptibility pattern, biochemical characteristics, and biotype number of C. braakii. Read here for the more details.

# data[].attributes.headline
	
	
	
	
	Energy Healing Treatment: C. braakii
	

# data[].attributes.subject
	
	
	
	
	Energy Healing Treatment: C. braakii
	

# data[].attributes.body
The study was proposed to evaluate the effect of energy healing treatment on Citrobacter braakii. Visit the provided link for more information.

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	
	
		Impact of Unique Energy Treatment on C. braakii
	

# data[].attributes.subject
	
	
	
	
	
		Impact of Unique Energy Treatment on C. braakii
	

# data[].attributes.body
The study was aimed to evaluate the changes in C. braakii characteristics after the unique energy treatment. The antimicrobial susceptibility assay, biochemical characteristics and biotyping analysis were evaluated.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Research ArticleOpen Access

Trivedi et al., J Clin Med Genom 2015, 3:1

http://dx.doi.org/10.4172/jcmg.1000129

Research ArticleOpen Access

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

Journal of Clinical & Medical Genomics

Keywords: Citrobacter braakii; Antimicrobial susceptibility; Bioeld

treatment; Biochemical reaction; Biotype; 16S rDNA analysis; Gram-

negative bacteria; Enterobacteriaceae

Abbreviations: MDR: Multi-Drug Resistant; ATCC: American Type

Minimum Inhibitory Concentration;OTUs: Operational Taxonomic

Units;NCBI: National Center for Biotechnology Information;MEGA:

Molecular Evolutionary Genetics Analysis; PCR: Polymerase Chain

Reaction; RDP: Ribosomal Database Project; HBMEC: Human Brain

Microvascular Endothelial Cells

Citrobacter braakii (C. braakii) is a genus of Gram-negative, straight,

facultative anaerobic and motile bacilli bacterium widely distributed in

water, soil, and food in the environment. It is also commonly found in

urinary, intestinal, and respiratory tract of human and animals, belongs

to Enterobacteriaceae family. It has been associated with various

nosocomial and community acquired infections in humans [1]. Arens

et al. reported about 11 genetically distinct species within the genus

Citrobacter [2]. e main clinical manifestations have been reported

due to nosocomial infections of Citrobacter species such as bacteremias

[3], endocarditis [4], urinary tract infections [1], neonatal meningitis

[5], pneumonia [6] and brain abscess [7]. Based on literature it has

been demonstrated that 0.8% of Gram-negative infections caused by

causes nosocomial infection among all Enterobacteriaceae family

[9]. Although it has low virulence property responsible for host cell

invasion instead of it invade blood brain barrier (BBB) of human brain

microvascular endothelial cells (HBMEC) and causes meningitis.

Moreover, due to overproduction of chromosomal β-lactamase

enzyme leads to antimicrobials resistance [10]. Aminoglycosides,

uoroquinolones, carbapenems, new oral cephems and many third and

fourth-generation cephems, such as cefepime and cefpirome, are the

drugs of choice to treat C. braakii associated infections but it possess

high level of resistance against penicillin and other antibiotics[11,12].

erefore, an alternative strategy is needed to alter the antimicrobial

sensitivity prole against C. braakii strain. In recent years, bioeld

treatment was proved to be an alternative method which has impact on

various properties of living and non-living materials in a cost eective

manner. It is already demonstrated that energy can neither be created

nor be destroyed but it can be transferred through various processes such

as thermal, chemical, kinetic, nuclear, etc. [13-15]. Similarly, electrical

current exists inside the human body in the form of vibratory energy

particles like ions, protons, and electrons and they generate magnetic

eld in the human body [16,17]. Aerward, Harold Saxton Burr had

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research

Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad

Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91 755 666 0006; E-mail:

Received August 20, 2015; Accepted September 23, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, et al. (2015)

braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study

was attempted to investigate the effect of Mr. Trivedi's bioeld treatment on C. braakii in lyophilized as well as

revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized

vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further

subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed

on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes.

First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were

studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample

was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial

susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated

cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to

susceptible after bioeld treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the

treated samples as compared to the control. Biochemical reactions also showed signicant (42.42%) alteration in

the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA

(53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus)

on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species

were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and

(53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed

that the identied microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The

results suggested that bioeld treatment has a signicant impact on C. braakii in lyophilized as well as revived state.

Phenotyping and 16S rDNA Analysis after Biofield Treatment on

1Trivedi Global Inc., Eastern Avenue Suite A-969, Henderson, NV, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Chinar Fortune City, Hoshangabad Rd., Madhya Pradesh, India

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

performed the detailed studies on the correlation of electric current

with physiological process and concluded that every single process in

the human body had an electrical signicance [18]. Recently, it was

discovered that all electrical process happening in body have strong

relationship with magnetic eld as mentioned by Ampere's law, which

states that the moving charge produces magnetic elds in surrounding

space [19,20]. us, the human body emits the electromagnetic waves

in form of bio-photons, which surround the body and it is commonly

known as bioeld. erefore, the bioeld consists of electromagnetic

eld, being generated by moving electrically charged particles (ions,

cell, molecule, etc.) inside the human body. According to Rivera-Ruiz

et al., it was reported that electrocardiography has been extensively

used to measure the bioeld of human body [21]. us, human has the

ability to harness the energy from environment or universe and can

transmit into any living or nonliving object(s) around the Globe. e

objects always receive the energy and responding into useful way that

is called bioeld energy and the process is known as bioeld treatment.

Mr. Trivedi's unique bioeld treatment (e Trivedi eect®) has been

known to transform the structural, physical and thermal properties of

several metals and ceramic in materials science [22-24], improved the

overall productivity of crops [25,26], altered characteristics features of

microbes [27-29] and improved growth and anatomical characteristics

of medicinal plants [30,31].

Due to the clinical signicance of this organism and literature on

bioeld treatment, the present work was undertaken to evaluate the

impact of bioeld treatment on C. braakii in relation to antimicrobials

susceptibility and biotyping based on various biochemical characters

followed by 16S rDNA sequencing analysis.

C. braakii, American Type Culture Collection (ATCC 43162) strain

was procured from MicroBioLogics, Inc., USA and stored with proper

storage conditions until further use. All the tested antimicrobials and

biochemicals were procured from Sigma-Aldrich (MA, USA). e

antimicrobial susceptibility, biochemical reactions and biotype number

were estimated with the help of MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA, USA) using Negative Breakpoint Combo

30 (NBPC 30) panel with respect to control group (Gr.). e 16S rDNA

sequencing study was carried out using ultrapure genomic DNA prep

kit; Cat KT 83 (Bangalore Genei, India).

e impact of bioeld treatment on tested bacterium C. braakii was

Group I: ATCC strain was revived from lyophilized state and

considered as control. No treatment was given and analyzed for

antimicrobial sensitivity, biochemical reactions and biotype number as

per the standard protocol.

two parts named as Gr. IIA and Gr. IIB. Both the groups of ATCC

strain of C. braakii in lyophilized state were assigned to the Mr. Trivedi's

unique bioeld treatment (rst treatment). Gr. IIB sample was stored in

lyophilized state for 159 days at -70ºC. Gr. IIBwas further sub-divided

in two separate parts named as Gr. IIB - Study Iand Gr. IIB - Study II.

Aer 159 days, antimicrobial sensitivity, MIC, biochemical

reactions and biotyping were performed as per the standard protocol.

e stored strain was revived from -70ºC and the revived culture

was again provided to Mr. Trivedi's bioeld treatment (re-treatment)

on day 159. Aer bioeld retreatment, the sample was sub-cultured

into three separate tubes on 3 dierent days (Day 0, Day 5 and Day

10) and analysed keeping the main treated tube aside. Each sample was

analyzed aer 5 days of its sub-culturing.

Bioeld treatment strategy

e lyophilized (Gr. IIA) sample of C. braakii was subjected to Mr.

Trivedi's bioeld treatment (rst treatment) followed by retreatment

aer storing for 159 days in revived state (Gr. IIB, Study II). In details,

the treatment groups in sealed pack were handed over to Mr. Trivedi for

bioeld treatment under laboratory conditions. Mr. Trivedi provided

the treatment through his energy transmission process to the treated

groups without touching the samples. Aer rst treatment, the analysis

of Gr. IIA lyophilized sample was done on day 10 for antimicrobial

sensitivity along with minimum inhibitory concentration (MIC),

biochemical reactions with biotype number and 16S rDNA analysis as

per the standard protocol. While handing over these cultures to Mr.

Trivedi for retreatment purposes, optimum precautions were taken to

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of C. braakii was

carried out with the help of automated instrument, MicroScan Walk-

Away® using NBPC 30 panel. e panel can be stored at 2 to 25ºC for

analysis. e panel was allowed to equilibrate to room temperature

prior to rehydration. All opened panels were used on the same day.

e tests carried out on MicroScan were miniaturized of the broth

dilution susceptibility test that has been dehydrated. Briey, 0.1 mL

of the standardized suspension of C. braakii was pipetted into 25

mL of inoculum water using pluronic and inverted 8 to 10 times and

inoculated, rehydrated, and then subjected to incubation for 16 hours at

35°C. Rehydration and inoculation was performed using the RENOK®

system with inoculators-D (B1013-4). 25 mL of standardized inoculum

suspension was poured in to inoculum tray. e detailed experimental

procedure and conditions were followed as per the manufacturer's

instructions. e antimicrobial susceptibility pattern (S: Susceptible,

R: Resistant; and I: Intermediate) and MIC values were determined by

observing the lowest antimicrobial concentration showing inhibition

Biochemical reaction studies

Biochemical reactions of C. braakii were determined using

MicroScan Walk-Away®, system with NBPC 30 panel. Preparation of

NBPC 30 panel, inoculum followed by dehydration and rehydration

were performed in a similar way as mentioned in antimicrobial

susceptibility assay for analysis of biochemical reactions followed by

biotype number. e detailed experimental procedures and conditions

were followed as per the manufacturer's instructions [32].

Identication of organism by biotype number

e biotype number of C. braakii was determined on MicroScan

Walk-Away® processed panel data report with the help of biochemical

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from C. braakii cells (Gr. IIA, sample coded

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

as 4A) using genomic purication kit, according to the manufacturer

instructions. 16S rDNA gene (~ 1.5 kb) fragment was amplied with the

help of high-delity polymerase chain reaction (PCR) using universal

primers; forward primer (5'-AGAGTTTGATCCTGGCTCAG-3') and

reverse primer (3'-ACGGTCATACCTTGTTACGACTT-5'). Amplied

products were subjected to gel electrophoresis in 1.0% agarose gel,

stained with ethidium bromide and visualized under UV light in a gel

documentation unit (BioRad Laboratories, USA). e PCR amplied

fragment was puried from the agarose gel using a DNA gel extraction

kit. Sequencing of amplied product was done on commercial basis

from Bangalore Genei, India. e 16S rDNA sequences obtained were

aligned and compared with the sequences stored in GenBank database

available from National Center for Biotechnology Information (NCBI)

using the algorithm BLASTn program. Multiple sequence alignment/

phylogenetic tree were established using MEGA3.1 molecular soware

Antimicrobial susceptibility test

e results of C. braakii susceptibility pattern and MIC values of

tested antimicrobials aer bioeld treatment are presented in Table 1

and 2 respectively. e data were analyzed and compared with respect

to control (Gr. I). Antimicrobial susceptibility assay was carried out

using twenty-eight antimicrobials. Overall, the treated cells of C.

braakii showed 39.29% alteration in antimicrobial sensitivity pattern

as compared to control. e sensitivity pattern of tetracycline was

changed from resistance (R) to susceptible (S) and simultaneously

decreased MIC value by two folds (>8 to ≤ 4 µg/mL) in all the treated

groups as compared to control (Gr. I). is improvement with

respect to resistant pattern and MIC value could be due to bioeld

treatment. e eect was observed throughout the experiment. So,

it may assume that the eect of bioeld treatment was sustainable.

Moreover, the antibiogram pattern of certain antimicrobials viz.

aztreonam, cefotaxime, cefotetan, ceazidime, ceriaxone, cefuroxime,

piperacillin/tazobactam, piperacillin and ticarcillin/k-clavulanate were

changed from inducible β-lactamase (IB) to susceptible in lyophilized

treated (rst treatment) Gr. IIB, Study I on day 159 as well as in revived

treated (second treatment) Gr. IIB, Study II on day 5 as compared

to untreated sample (Gr. I). e microbe C. braakii has the ability to

produce chromosomal β-lactamases. us, the overproduction of

this enzymes lead to resistance in most of penem and cephems ring

containing antimicrobials [34].

However, aer bioeld treatment the above mentioned nine

antimicrobials were converted from IB to completely susceptible

in lyophilized treated (rst treatment) Gr. IIB, Study I on day 159 as

well as in revived treated (second treatment) Gr. IIB, Study II on day

5 as compared to control sample. However, the susceptibility pattern

of these nine antimicrobials did not show any alteration in rest of

the treated samples as compared to control. is alteration could be

due to exertion of bioeld energy to the treated samples at enzymatic

S. No.AntimicrobialGr. I (Control)Gr. IIA (Day 10)Gr. IIB (Study I; Day

Gr. IIB (Study II; Day 159)

3.Ampicillin/sulbactamI I I I I I

4.AmpicillinR R R R R R

8. CefotaximeIB IB S S IB IB

12. CeftriaxoneIBIBSSIBIB

15.Chloramphenicol S S S S S S

16. Ciprooxacin S S S S S S

18. GentamicinS S S S S S

20. Levooxacin S S S S S S

21.MeropenemS S S S S S

24.PiperacillinIB IB SSIB IB

25.TetracyclineR S S S S S

26. Ticarcillin/k-clavulanateIBIBSSIBIB

27.TobramycinS S S S S S

/sulphamethoxazole S S S S S S

R: Resistant; S: Susceptible; I: Intermediate; IB: Inducible β-lactamase; Gr.: Group

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii - PdfSR.com

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	
	
		Study of Biochemical Reaction on Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	
		Study of Biochemical Reaction on Citrobacter braakii
	

# data[].attributes.body
The determination of biochemical reactions of Citrobacter braakii shows the effects of biofield treatment. Get more information about this research.

# data[].attributes.attachment_link.keywords[]
16s rdna analysis of citrobacter braakii

# data[].attributes.attachment_link.title
"Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	
	
		Phenotyping and 16S rDNA Analysis of C. braakii
	

# data[].attributes.subject
	
	
	
	
	
		Phenotyping and 16S rDNA Analysis of C. braakii
	

# data[].attributes.body
Biofield energy treatment, also known as alternative therapy was used in this study to determine the impact on the phenotyping & 16S rDNA analysis of C. braakii.

# data[].attributes.headline
	
	
	
	
	16S rDNA Sequencing of Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	16S rDNA Sequencing of Citrobacter braakii
	

# data[].attributes.body
The 16S rDNA sequencing of Citrobacter braakii shows that the biofield treatment could be used as an alternate of existing drug therapy in future. Read here for more details.

# data[].attributes.headline
	
	
	
	
	
		 Antimicrobial susceptibility test on Citrobacter braakii
	

# data[].attributes.subject
	
	
	
	
	
		 Antimicrobial susceptibility test on Citrobacter braakii
	

# data[].attributes.body
In conclusion, the antimicrobial susceptibility pattern and MIC values showed 39.29% and 15.63% alteration, respectively of tested antimicrobials as compared to the control strain of C. braakii.

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for

# data[].attributes.headline
 Phenotyping Analysis of Citrobacter braakii after Biofield
	

# data[].attributes.subject
 Phenotyping Analysis of Citrobacter braakii after Biofield
	

# data[].attributes.body
Read here about the phenotyping analysis of Citrobacter braakii after biofield energy treatment.

# data[].attributes.headline
	
	
	
	
	
		Citrobacter braakii | Mr. Trivedi’s Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	
		Citrobacter braakii | Mr. Trivedi’s Biofield Treatment
	

# data[].attributes.body
The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, and biochemical characteristics.

# data[].attributes.headline
	
	
	
	
	
		Effect of Spiritual Energy on Yersinia enterocolitica
	

# data[].attributes.subject
	
	
	
	
	
		Effect of Spiritual Energy on Yersinia enterocolitica
	

# data[].attributes.body
Mr. Trivedi’s distinctive human energy has altered the Yersinia enterocolitica biochemical reactions along with the antibiotic susceptibility. Visit to get more information about the test and results.

# data[].attributes.headline
	
	
	
	
	
		Study of Yersinia enterocolitica [ATCC–23715]
	

# data[].attributes.subject
	
	
	
	
	
		Study of Yersinia enterocolitica [ATCC–23715]
	

# data[].attributes.body
The result indicates that Mahendra Trivedi‘s energy has changed the biochemical characteristics, susceptibility pattern & Biotype number of Yersinia enterocolitica.

# data[].attributes.attachment_link.title
Impact of an external energy on Yersinia enterocolitica [ATCC -23715] in relation to antibiotic susceptibility and biochemical reactions: An experimental study

# data[].attributes.attachment_link.description
While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC -23715], report

# data[].attributes.headline
	
	
	
	
	
		Biochemical Reaction- Yersinia enterocolitica
	

# data[].attributes.subject
	
	
	
	
	
		Biochemical Reaction- Yersinia enterocolitica
	

# data[].attributes.body
This research showed that Mr. Trivedi’s energy has altered the biochemical reactions along with the antibiotic susceptibility of Yersinia enterocolitica only within the period of 10 days.

# data[].attributes.headline
	
	
	
	
	
		16s rDNA Analysis of Yersinia enterocolitica
	

# data[].attributes.subject
	
	
	
	
	
		16s rDNA Analysis of Yersinia enterocolitica
	

# data[].attributes.body
In this experiment, the 16s rDNA tests show a change in the bacterial species. Follow the given link to know more.

# data[].attributes.headline
	
	
	
	
	
		 Yersinia enterocolitica | Impact of External Energy
	

# data[].attributes.subject
	
	
	
	
	
		 Yersinia enterocolitica | Impact of External Energy
	

# data[].attributes.body
The present experiment on Yersinia enterocolitica report the effects of such energy transmitted through a person, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.attachment_link.title
"Impact of an external energy on Yersinia enterocolitica [ATCC -23715] in relation to antibiotic susceptibility and biochemical reactions: An experimental study" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Background: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC -23715], report the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	Study of Antifungal Sensitivity of Fungi
	

# data[].attributes.subject
	
	
	
	
	Study of Antifungal Sensitivity of Fungi
	

# data[].attributes.body
The study evaluates the impact of biofield treatment on pathogenic species of Fungi. The results show the changes in antifungal sensitivity of fungi.

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Volume 5 * Issue 2 * 1000125

ISSN: 2165-8056 FGB, an open access journal

Fungal Genomics & Biology

Trivedi et al., Fungal Genom Biol 2015, 5:2

http://dx.doi.org/10.4172/2165-8056.1000125

Evaluation of Antifungal Sensitivity Assay of Biofield Energy

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052,USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

*Corresponding author:Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal- 462026, Madhya Pradesh, India; Tel no: +91-755-6660006; E-mail:

Evaluation of Antifungal Sensitivity Assay of Bioeld Energy Treated Fungi. Fungal

Genom Biol 5: 125. doi:10.4172/2165-8056.1000125

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated

the impact of bioeld treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern.

Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized).

Treatment groups received the bioeld treatment, and control group was remained as untreated. Mini-API ID32C

strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results

showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to

resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell

from S→I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs:

uconazole from S→I (T1 on day 10) and itraconazole S→I (T1 and T2 on all assessment days). The Cryptococcus

altered from S→I (T1) and S→R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8

folds, as compared to the control. Fungal identication data showed the signicant changes in species similarity of

few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10.

C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day

5) and 99.0% (T2). These ndings suggest that bioeld treatment could be applied to alter the susceptibility pattern

of antifungal drug therapy in future.

Keywords: Fungi; Amphotericine B; Fluconazole; Flucytosine;

e kingdom fungi includes yeast, mold, and mushrooms that have

multiple applications like fermentation of sugar and bear; productions

of antibiotics and enzymes; as biological pesticide; and also consumed

as food products like mushrooms, morels, and trues. Apart from

their benecial applications, the fungi are also responsible for several

topical and systemic diseases in living organisms. Some of the fungi

also produce toxic chemicals like acetaldehyde that promotes free

radicals generation in human body. is acetaldehyde also converted

into ethanol by liver, and people report feeling like a drunk or hangover

with debilitating fatigue [1,2]. C. albicans is an opportunistic fungus

and commonly situated in the intestinal tract. It causes infection during

the favorable condition and responsible for candidiasis in human.

It produces secretory enzymes like phospholipase, proteinase, etc.

that play a major role in the pathogenicity [3]. C. kefyr is uncommon

but considered as non-pathogenic fungus so far. However, due to

its continuous resistance towards the existing antifungal drugs, it

suddenly considered as an emerging pathogen, especially in patients

with risk factors like immune-compromised and AIDS patients [4].

C. krusei is budding yeast that is mainly used in chocolate production

to remove the bitter taste of cocoa butter. Apart from this, it is an

emerging nosocomial fungus and mainly found in the immune-

compromised patients. Currently, C. krusei has been recognized as a

potentially multidrug-resistant fungal pathogen, owing to its natural

uconazole resistance along with decreased susceptibility to ucytosine

and amphotericin B [5]. C. neoformans is unicellular opportunistic

yeast, and its infection in human called as cryptococcosis. Generally,

cryptococcosis occurs in lungs but fungal meningitis and encephalitis

also reported in some AIDS patients [6]. C. tropicalis is a yeast that

usually responsible for human diseases in tropical countries especially

in immune-compromised and immune-competent patients. Recently,

infection of C. tropicalis has increased considerably worldwide,

particularly against the recognized antifungal drug uconazole [7]. S.

cerevisiae is a type of yeast and plays a central role in the spoilage of

foods and beverages [8]. However, despite of their benecial eects in

food and beverage industries, these fungi are harmful also to human

being. Recently, bioeld treatment is reported to alter the sensitivity of

antimicrobials against several microbes [9,10].

Energy is the property of matter that neither can be created nor

destroyed, however it can be transmitted to other substances by changing

into dierent forms. According to the Einstein's equation (E=mc2) the

energy and mass are fundamentally related to each other. As like to

energy in solid matter in the form of vibratory atoms or molecule; the

human body also have the vibratory energy particles like neutrons,

protons, and electrons [11,12]. e famous scientist Willem Einthoven

developed an electrocardiography (ECG) in 1924 to measure the bioeld

of human body. Harold Saxton Burr gave the hypothesis that every

single process in the human body has an electrical impact. Recently, it

was reported that all the electrical process happening in body generates

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Bairwa K, et al. (2015) In Vitro Evaluation of Antifungal Sensitivity Assay of Bioeld Energy

Treated Fungi. Fungal Genom Biol 5: 125. doi:10.4172/2165-8056.1000125

Volume 5 * Issue 2 * 1000125

ISSN: 2165-8056 FGB, an open access journal

magnetic eld that can be accessed by using some medical technologies

such as electrocardiography (ECG), electromyography (EMG), and

electroencephalogram (EEG). erefore, the electromagnetic eld

generated from human body known as bioeld and energy associated

with this eld is known as bioeld energy [13-15]. Mr. Mahendra

Trivedi's bioeld treatment (e Trivedi Eect®) is known to alter

the crystalline, thermal, and atomic properties of various metals and

ceramics [16-18]. e growth and anatomical characteristics of some

herbs were also changed considerably aer bioeld treatment [19].

In agriculture, the bioeld treated crops have shown signicant eect

on growth, characteristics and yield [20]. Further, the changes in

antimicrobial susceptibility and biotype number of some microbes have

been reported aer bioeld treatment [9,10,21]. In the present work,

authors evaluated the eect of bioeld treatment on dierent species of

fungi in relation to antifungal drug sensitivity.

e lyophilized samples of fungi [Candida albicans (ATCC

66029), and Saccharomyces cerevisae (ATCC 9763)] were procured

from MicroBioLogics, Inc., USA. ese lyophilized samples were stored

as per the suggested storage conditions until further use. e antifungal

susceptibility study and minimum inhibitory concentration (MIC) of

all the fungal samples were carried out using mini API® ID32C strip.

e antifungal drugs: amphotericine B, uconazole, ucytosine, and

itraconazole were procured from Sigma-Aldrich, MA, USA.

e lyophilized fungi cells were divided into three groups: C, control;

T1, treatment (revived); T2 treatment (lyophilized). e control group

was remained as untreated; and treatment sample was handed over

in sealed pack to Mr. Trivedi for bioeld treatment under laboratory

conditions. Mr. Trivedi provided the bioeld treatment through his

energy transmission process to the treatment group without touching

the sample. Aer that, groups C and T1 were analyzed on day 5 and 10,

while group T2 was examined on day 10, for antifungal drug sensitivity.

Investigation of antifungal sensitivity and fungal strain

e antifungal drug susceptibility and fungi identication were

performed on mini API® ID 32C strips (ATB system) with MIC break

point by observing the lowest antifungal concentration showing

growth inhibition [22]. API® ID 32C strips a simple and standardized

method. It is widely accepted for identication of clinically important

food and water-associated microbes using standardized biochemical

tests. For every tested fungus, the biochemical reactions were analyzed

by colorimetric assay. Based on this colorimetric assay, the digital

analytical prole index (API) code was calculated. ese codes then

fed into the on-line ApiwebTM database, which gave the nal output

in the form of microbe identication [23]. e sensitivity study was

performed using four antifungal drugs: amphotericine B, uconazole,

ucytosine, and itraconazole.

e results of sensitivity pattern (resistant, R; intermediate, I; or

susceptible, S) and MIC values of antifungal drugs are summarized in

Table 1 and 2, respectively. e antifungal sensitivity results showed

Candida albicansFluconazole

Candida kefyrFluconazole

Candida kruseiFluconazole

Cryptococcus neoformansFluconazole

Candida tropicalisFluconazole

Saccharomyces cerevisiaeFluconazole

C- Control; T- Treated; I- Intermediate; S- Susceptible; R-Resistant.

The NCCLS recommended breakpoints (in mg/L) for Fungus species are as

following: Flucytosine: S (≤ 4), I

(8-16), R (≥ 32); Fluconazole: S (≤ 8), I (16-32), R (>64); Itraconazole: S (<0.125),

Table 1: Effect of bioeld treatment on fungal sensitivity.

Candida albicansAmphotericine B <0.5<0.5<0.5 <0.5

Flucytosine <0.5<0.5 <0.5 <0.5

Itraconazole <0.125 <0.125 <0.125

Itraconazole <0.125 <0.1250.5

C-Control; T-Treated; MIC data are presented in µg/mL.

The NCCLS recommended breakpoints (in mg/L) for Fungus species are as

following: Flucytosine: S (≤ 4), I (8-16), R (≥ 32); Fluconazole: S (≤ 8), I (16-32), R

(>64); Itraconazole: S (<0.125), I (0.25-0.5), R (≥ 1).

Table 2: Effect of biofeild treatment on minimum inhibitory concentration (MIC) of

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Bairwa K, et al. (2015) In Vitro Evaluation of Antifungal Sensitivity Assay of Bioeld Energy

Treated Fungi. Fungal Genom Biol 5: 125. doi:10.4172/2165-8056.1000125

Volume 5 * Issue 2 * 1000125

ISSN: 2165-8056 FGB, an open access journal

that susceptibility of C. albicans was changed from I→S and I→R against

itraconazole in T1 cells on day 5 and 10, respectively. However, it did not

show any alteration in susceptibility patterns against uconazole, and

ucytosine as compared to control. e sensitivity of C. kefyr converted

from S→I in T2 cell on day 10 against itraconazole only out of four tested

antifungal drugs. e C. krusei showed the alteration in sensitivity of two

antifungal drugs, i.e., uconazole from S→I (T1 on day 10) and itraconazole

S→I (T1 and T2 on all assessment days); however, the sensitivity to

ucytosine remained same as control. Likewise, the C. neoformans was

changed from S→I in T1 cell on day 5 and 10 against to itraconazole.

Further, the C. neoformans did not show alterations in sensitivity patterns

of remaining antifungal drugs with respect of control. e sensitivity of C.

tropicalis was altered from I→R against ucytosine in both group T1 and

T2 on all assessment days. Further, it showed the similar sensitivity pattern

for uconazole, and itraconazole as control. e sensitivity of S. cerevisiae

was altered from S→I and S→R against itraconazole in T1 and T2 cells,

respectively, on day 10; despite these, the sensitivity patterns against other

three tested antifungal drugs were remained same as control. e sensitivity

pattern of amphotericine B was not dened using mini API® method.

e MIC results of all tested antifungal drugs against six tested fungi

are reported in Table 2. C. albicans showed the 4-folds increase in MIC

value of uconazole and itraconazole in T1 cells on day 10, as compared

to control. It also showed the 2-folds increase in MIC value of uconazole

in T2 cell on day 10. e C. kefyr showed the 2-folds increase in MIC

value of itraconazole in T2 cells with respect to control. A signicant

decrease (2-folds) in MIC values of amphotericine B and uconazole was

observed in T2 cells of C. krusei as compared to control. However, it also

showed the 2-folds increase in MIC value of uconazole in T1 cells on

day 10 with respect to control. e C. neoformans showed the 2-folds

increase in MIC of ucytosine and itraconazole in T1 cells on day 5 and

10 as compared to the control; additionally, 4-folds increase in MIC value

of ucytosine was also observed in T2 cells of C. neoformans with respect

to control. e treated cells of C. tropicalis showed 2-folds increase in

MIC value of ucytosine in T2 group and 2-fold increase in MIC value of

itraconazole in both T1 and T2 groups, with respect to control. Similarly,

the treated cells of S. cerevisiae showed the increase in MIC of uconazole

(2 fold in T1 and T2 on day 10) and itraconazole (4 and 8 fold in T1 and

T2, respectively on day 10) as compared to control (Table 2).

Fungal strain identication

e data of fungal strain identication study revealed the signicant

change in the identication of fungi sample like the species similarity

of C. albicans was altered from 91% to 98.5% and 99.9% in T1 and T2,

respectively on day 10 as compared to control. C. krusei exhibited a

7 alteration in species similarity from 97.9% to 85.9% in group T2.

e species similarity of C. tropicalis was found to alter from 88.7% to

99.6% in group T1 on day 5, and 99.0% in group T2. Despite these, no

other fungi showed the signicant alterations in species similarity, as

compared to control (Table 3).

Over the past few decades, the persistent fungal infections have

been emerged as a prominent cause of morbidity and mortality in

immune-compromised patients. Unfortunately, huge consumption

of triazoles in prophylactic and empiric antifungal therapy caused to

selective drug-resistant against candida and aspergillus species [24].

However, the naturally occurring resistance in fungi is very rare and

slow as compared to the viral and bacterial resistance. e overall

resistance in candida species against uconazole and voriconazole is

around 3-6% and it remained constant over a decade. Similarly, triazole

resistance in A. fumigatus has been increasingly recognized about 6%

over the past few years [24-26]. erefore, severity of fungal infection

is increasing recently and become more crucial due to the emergence

of multidrug resistance against some antifungal drugs. Hence, in the

present study we aimed to evaluate the eect of bioeld treatment on

six dierent species of fungi and analyzed against antifungal sensitivity

and fungal strain identication.

All fungi including Candida and Saccharomyces are usually

susceptible to polyene and azole antifungal drugs [27,28]. e present

study also suggested the similar type of sensitivity pattern in the control

samples of tested fungi. e antifungal sensitivity results showed

that sensitivity of itraconazole against C. albicans was changed from

intermediate to susceptible in group T1 on day 5. at was further

converted to resistant on day 10 (Table 1). Likewise, the other tested

fungi except C. tropicalis have showed the altered sensitivity patterns to

itraconazole aer bioeld treatment (Table 1). e MIC values of tested

antifungal drugs were increased in the range of 2-8 folds as compared

to control (Table 2). e results of ATB identication method revealed

the change in species homology like C. albicans was identied as

91.9 % in control that was increased to 98.9 and 99.99% in group T1

tropicalis also showed the signicant change in the species homology

as compare to control (Table 3). Overall, these results revealed that the

changes might be occurred due to some alterations in the enzymatic

or metabolic pathway or changes at cellular level of tested fungi aer

bioeld treatment [29,30]. To the best of our knowledge, this is the rst

report describing the impact of bioeld on the antifungal sensitivity

and species homology of fungi cells.

Altogether, the present study concludes that antifungal sensitivity

and MIC values were altered aer bioeld treatment of tested six fungi.

e species homology of few tested fungi was also changed in treated

sample that may be due to some alteration at the genomic level or

metabolic pathway of fungi. ese observations suggested that bioled

treatment could be helpful to alter the susceptibility of fungi against the

existing antifungal drugs.

The authors would like to acknowledge the whole team of Hinduja Microbiology

Lab for their support. We would also like to thank Trivedi Master Wellness™, Trivedi

Science™, and Trivedi Testimonials for their consistent support during the work.

1. Mullar GM, Bills GF, Foster MS (2004) Biodiversity of fungi: Inventory and

monitoring method, Elsevier academic press, London, UK.

2. Deacon JW (2006) Fungal biology, Blackwell publishing, Massachusetts, USA.

3. Ito KCY, Lyon JP, Vidotto V, de Resende MA (2006) Virulence factors and

antifungal susceptibility of Candida albicans isolates from oral candidosis

patients and control individuals. Mycopathologia 161: 219-223.

Table 3: Fungal strain identication.

# data[].attributes.attachment_link.title
In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi - PdfSR.com

# data[].attributes.attachment_link.description
Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized).

# data[].attributes.headline
	
	
	
	
	
		Antifungal Sensitivity Assay of Human Energy Treated Fungi
	

# data[].attributes.subject
	
	
	
	
	
		Antifungal Sensitivity Assay of Human Energy Treated Fungi
	

# data[].attributes.body
The antifungal sensitivity assay suggests that the unique healing treatment could be helpful to alter the susceptibility of fungi against the existing antifungal drugs. For more details visit the link.

# data[].attributes.attachment_link.keywords[]
antifungal susceptibility testing for candida

# data[].attributes.attachment_link.title
"In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized).

# data[].attributes.headline
	
	
	
	
	
		Investigation of Antifungal Sensitivity of Fungi
	

# data[].attributes.subject
	
	
	
	
	
		Investigation of Antifungal Sensitivity of Fungi
	

# data[].attributes.body
The study was performed to investigate the antifungal sensitivity pattern of fungi, in the presence of spiritual energy treatment. Read here the results.

# data[].attributes.headline
	
	
	
	
	In Vitro Evaluation of Fungi for Antifungal Sensitivity
	

# data[].attributes.subject
	
	
	
	
	In Vitro Evaluation of Fungi for Antifungal Sensitivity
	

# data[].attributes.body
| The study represents the in-vitro evaluation of antifungal sensitivity assay of human energy treated fungi. Visit the link for more details.

# data[].attributes.attachment_link.title
In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi

# data[].attributes.attachment_link.description
Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample

# data[].attributes.headline
	
	
	
	
	
		External Energy Impact on Antifungal Sensitivity Assay
	

# data[].attributes.subject
	
	
	
	
	
		External Energy Impact on Antifungal Sensitivity Assay
	

# data[].attributes.body
Due to the influence of Mahendra Trivedi's unique energy healing, there were some significant alterations in antifungal sensitivity assay of treated fungi. Visit the link for more details.

# data[].attributes.headline
	
	
	
	
	Antifungal Susceptibility Testing for Candida
	

# data[].attributes.subject
	
	
	
	
	Antifungal Susceptibility Testing for Candida
	

# data[].attributes.body
The study of Antifungal Susceptibility for Candida fungi was performed under the influence of unique human energy treatment. Read here for more details.

# data[].attributes.headline
	
	
	
	
	
		Antibiotic Susceptibility of Staphylococcus Epidermis
	

# data[].attributes.subject
	
	
	
	
	
		Antibiotic Susceptibility of Staphylococcus Epidermis
	

# data[].attributes.body
The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of energy healing therapy transmitted through Mr Mahendra Trivedi, in related to antibiotic susceptibility and biochemical reactions.

# data[].attributes.attachment_link.title
Impact of an external energy on Staphylococcus epidermis [ATCC -13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study

# data[].attributes.attachment_link.description
Purpose: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC -13518], validate the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	Effect of Energy Treatment on S. Epidermis
	

# data[].attributes.subject
	
	
	
	
	Effect of Energy Treatment on S. Epidermis
	

# data[].attributes.body
Due to the effect of unique energy treatment of S. Epidermis, there was an alteration in antibiotic susceptibility and biochemical reaction pattern was observed in treated sample. For more information visit the link.

# data[].attributes.attachment_link.title
"Impact of an external energy on Staphylococcus epidermis [ATCC -13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Purpose: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC -13518], validate the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner.

# data[].attributes.headline
	
	
	
	
	
		 Staphylococcus Epidermis' Biofield Treatment
	

# data[].attributes.subject
	
	
	
	
	
		 Staphylococcus Epidermis' Biofield Treatment
	

# data[].attributes.body
In this research, the Staphylococcus Epidermis is treated with the biofield energy treatment by Mahendra Trivedi and analysed for the changes in various properties like susceptibility pattern and etc.

# data[].attributes.headline
	
	
	
	
	Biochemical Characteristics of Staphylococcus Epidermis
	

# data[].attributes.subject
	
	
	
	
	Biochemical Characteristics of Staphylococcus Epidermis
	

# data[].attributes.body
The study of biochemical characteristics shows the alterations along with a significant change in susceptibility pattern of treated sample after the influence of human energy treatment.

# data[].attributes.attachment_link.title
Impact of an external energy on Staphylococcus epidermis [ATCC -13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study

# data[].attributes.attachment_link.description
While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC -13518],

# data[].attributes.headline
	
	
	
	
	
		 Evaluation of Staphylococcus Epidermis Polymorphism
	

# data[].attributes.subject
	
	
	
	
	
		 Evaluation of Staphylococcus Epidermis Polymorphism
	

# data[].attributes.body
Read here the study which shows the impact of human energy treatment on Staphylococcus Epidermis polymorphism. Kindly visit to know more.

# data[].attributes.headline
	
	
	
	
	
		 Staphylococcus Epidermis Lyophilization
	

# data[].attributes.subject
	
	
	
	
	
		 Staphylococcus Epidermis Lyophilization
	

# data[].attributes.body
The Staphylococcus epidermis in the Lyophilized state shows the change in few characteristics of S. Epidermis. Follow this link for more information.

# data[].attributes.headline
	
	
	
	
	
		



	
	
	
	
	Staphylococcus Epidermis Antibiotic Susceptibility
	

# data[].attributes.subject
	
	
	
	
	
		



	
	
	
	
	Staphylococcus Epidermis Antibiotic Susceptibility
	

# data[].attributes.body
The study shows that due to the influence of spiritual energy treatment by Mr Trivedi, there was an alteration observed in antibiotic susceptibility pattern of Staphylococcus epidermis.

# data[].attributes.attachment_link.readable_text
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5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 1/10

JournalofAccordIntegrativeMedicine

Copyright©2008Allrightsreserved.Nopartofthispublicationmaybeusedorreproduced,storedina

retrievalsystem,ortransmittedinanyformsorbyanymeans,electronic,mechanical,photocopying,

recordingorotherwise,withoutwrittenpermission.

Nov.2008,Volume4,Number4

ImpactofanexternalenergyonStaphylococcus

epidermis[ATCC-13518]inrelationtoantibiotic

susceptibilityandbiochemicalreactions-An

Nov.2008,Volume4,Number4:230235

MahendrakumarTrivedi(B.E.Founder),ShrikantPatil(B.D.S.Associate)*

SocietyforDivineLife,A14,KanwalApartment,FourBungalows,Andheri(W.),

PinCode400058,Maharashtra-INDIA

MahendrakumarTrivedi:+919324871979

ShrikantPatil:+919869118769

Categoryofsubmission:InVitroStudy

Institutioninwhichtheworkwasperformed:

MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearch

Centre,Mumbai,India-accreditedbyTheCollegeofAmericanPathologists

5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 2/10

Keywords-Antibioticresistance,BiochemicalReactions,Lyophilization,

Polymorphism,Energy,Model

Whilespiritualandmentalenergiesareknowntoman,theirimpacthasneverbeen

scientificallymeasurableinthematerialworldandtheyremainoutsidethedomainof

science.ThepresentexperimentonStaphylococcusepidermis[ATCC-13518],

validatetheeffectsofsuchenergytransmittedthroughaperson,Mr.Mahendrakumar

Trivedi,whichhasproducedanimpactmeasurableinscientificallyrigorousmanner.

Staphylococcusepidermisstrainsinrevivedandlyophilizedstateweresubjectedto

spiritualenergytransmittedthroughthoughtinterventionand/orphysicaltouchofMr.

Triveditothesealedtubescontainingstrain,theprocesstakingabout3minutesand

wereanalyzedwithin10daysafterincubation.Alltestswereperformedwiththehelp

ofautomationontheMicroscanWalkawaySysteminMicrobiologyLaboratory

accreditedbyTheCollegeofAmericanPathologists.

TheresultsindicatedthatMr.Trivedi'senergyhaschanged7of27biochemical

characteristicsofStaphylococcusepidermisalongwithsignificantchangesin

susceptibilitypatternin8of29antibiotics.TheBiotypenumberhaschangedfromthe

originalcontrolstraingivingriseto2differentbiotypesintreatedsampleswhilethe

externalenergy/treatmentgivenwasthesameforalltreatedsamplessuggestiveof

randompolymorphismasanalyzedthroughtheautomatedmachine.

Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicateapotency

inMr.Trivedi'senergy,providingamodelforsciencetobeabletoinvestigatethe

impactofspiritualenergyinarigorousmanner.

Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.

Alllivingorganismscontainlargecomplexmoleculeswithinthecells.Someofthese

areproteinsandenzymesincludingDNA/RNA.Thenatureoftheorganismsis

dependentonthebiochemicalreactions,whichcanbecharacterizedandidentified.

5/14/2015 JournalofAccordIntegrativeMedicine

http://www.accordinstitute.org/2008_4_4_impact_of_an_external_energy_on_staphylo.htm 3/10

Antibioticresistance,regardlessofantibioticandbacteria,willoccurwithsufficient

timeanddruguse.Widespreadantibioticusecausesselectionpressure:resistantstrains

survivewhilesusceptibleonesareeliminated.Antibioticresistanceisprogressive,

increasingfromlowtointermediatetohighlevels[1].Bacterialstrainsarealso

characterizedbytheirrelativeresistancestoarangeofantibiotics.However,sucha

changedoesnottakeplacespontaneouslyinastrainovera10dayperiodofnormal

incubationwithoutcontinuousexposuretoanydrugs.

InthispaperwereporttheimpactofspiritualenergyonStaphylococcusepidermis ,in

revivedandlyophilizedstate,withrespecttoitsantibioticsusceptibilitypatternalong

withbiochemicalpropertiesanalyzedwithinaperiodof10days.Thesaidenergywas

transmittedthroughthoughtinterventionofanindividual,Mr.MahendrakumarTrivedi

whohasbeeninteractingwithalargenumberofpeopleasahealeroverthelastdecade.

Asresponsesbyhumanscanbeaccountedforbytheplaceboeffect,theseexperiments

onlowerorganismsweredesignedinordertodirectlytesttheimpactthroughscientific

studiestoruleouttheplaceboeffect.

Itiswidelyacceptedthatlyophilizationisthemethodmostcommonlyusedtostoreand

transportmicrobialculturesaschangeinthebiochemicalandenzymaticcharacteristics

ofanorganismcannotbecarriedoutinthisstate.

TwostrainsofStaphylococcusepidermis[ATCC-13518]wereprocuredfrom

MicroBioLogicsinsealedpacksbearingthesameATCCnumberandstoredaccording

totherecommendedstorageprotocolsuntilneededforexperiments.Thestudywas

groupedasperthefollowing.

OneofthetwosealedpacketswashandedovertoHindujaMicrobiologyLabandwas

revivedbythemintwoseparatetubes,ofwhichonewasthecontrol.Thecontroltube

wasanalyzedforidentification,antibioticsusceptibilityandbiochemicalreactionsas

perthestandardprotocolsofsampleprocessinginthemicrobiologylab.

Thesecondtube,(ATCC'A')havingviablebacterialculture,washandedoverto

Mr.Trivedifortreatment,aftersealingbyparafilm.Itwasassessedonthe5thand10th

Mr.Trivediheldthistubeinhishandunderambientconditionsforbetween0.5to3

# data[].attributes.attachment_link.title
Impact of an external energy on Staphylococcus epidermis [ATCC -13518] in relation to antibiotic susceptibility and biochemical reactions - An experimental study - PdfSR.com

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Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC -13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner.

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		Alternative Energy Treatment Analysis of M-Toluic Acid
	

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		Alternative Energy Treatment Analysis of M-Toluic Acid
	

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The impact of Human energy treatment is studied on m-Toluic Acid, the reactivity may be reduced slightly at the aromatic ring by a slight increase in the effective mass (μ) and the binding energy of the treated sample.

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American Journal of Applied Chemistry

Published online December 21, 2015 (http://www.sciencepublishinggroup.com/j/ajac)

doi: 10.11648/j.ajac.20150306.17

ISSN: 2330-8753 (Print); ISSN: 2330-8745 (Online)

Quantitative Determination of Isotopic Abundance Ratio of

13C, 2H, and 18O in Biofield Energy Treated Ortho and Meta

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal Madhya Pradesh,

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia Snehasis Jana. Quantitative Determination of Isotopic

O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers. American Journal of Applied Chemistry.

Vol. 3, No. 6, 2015, pp. 217-223. doi: 10.11648/j.ajac.20150306.17

O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic

intermediates, mostly used in medicines and pesticide s. The ai m of the study was to evaluate the impact of biofield energy

treatment on isotopic abundance ratios of

O, (PM+2)/PM, in toluic acid isomers using gas

chromatography-mass spectrometry (GC-MS). The OTA and MTA samples were divided into two parts: control and treated. The

control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The

treated group was subjected to biofield energy treatment. The GC-MS spectra of both the isomers showed five m/z peaks due to

the molecular ion peak and fragmented peaks of toluic acid derivatives. The isotopic abundance ratio of (PM+ 1)/PM and

(PM+2)/PM were calculated for both the isomers and found significant alteration in the treated isomers. The isotopic abundance

ratio of (PM+1)/PM in treated samples of OTA was decreased and then slightly increased upto 2.37% in T2, where the

(PM+2)/PM in treated OTA, significantly decreased by 55.3% in T3 sample. Similarly, in case of MTA, the isotopic abundance

ratio of (PM+1)/PM in the treated sample showed a slight increase the (PM+2)/PM was decreased by 11.95% in T2 as compared

to their respective control. GC-MS data suggests that the biofield energy treatment on toluic acid isomers had significantly

altered the isotopic abundance of

O in OTA and MTA as compared to the control.

Biofield Energy Treatment, o-Toluic Acid, m-Toluic Acid, Gas Chromatography-Mass Spectrometry

The distributio n of cont aminant source s of any molecule

on a native or global scale can be understood by determining

the isotopic abundance ratio, and character ization of

elementary reaction mechanisms [1]. The natural abundance

or any kinetic process that leads to the local d epletion or

enhancement of isotop es in organic molecules ca n be

successfully determined using gas chromatogra phy-mass

spectrometry (G C-MS) [2]. Moreover, the rate of chemical

reaction may vary with the mass of the nucleus with differ ent

isotopic subs titutions, whic h slightly affect the partitioning

of energy within the mo lecules. T hese deviations from

perfect chemica l equivalence are t ermed as isotope effect s.

The isotopic abundan ce ratio is commonly r eported in terms

of atom percent and determined by high reso lution mass

spectrometry (HRMS sp ectrometry) [3].

Two toluic acid derivatives, o-toluic acid (OTA) and

m-toluic acid (MTA) were taken for mass spectroscopy study.

Both of the toluic acid derivatives are widely used as a

chemical intermediate to synthesis the insect repellant in the

chemical industry, as plastic stabilizer in polymer industry and

as a starting material in pharmaceutical products [4, 5]. MTA

is used specifically as starting material in the production of

3-carboxybenzaldehyde, 3-methylbenzophenone,

3-benzoylphenylacetic acid, N,N-diethyl-3-methylbenzamide,

etc. [6]. They are mostly fou nd in crude oil and artificially

synthesized from toluidines and oxidation of ortho and meta

xylenes [7]. Besides, toluic acid isomers are biodegradable

218 Mahendra Kumar Trivedi et al.: Quantitative Determination of Isotopic Abundance Ratio of

O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers

products and its b ioaccumulation potential is very low as

compared to their homologs. This chemical is stable to

hydrolysis in water in abiotic conditions, however, indire ct

photo-oxidation may have occurr ed via hydroxy radicals in

the atmosphere. If xylene and toluene were taken with

contaminated food they are metabolized mainly to

eventually conjugated with glycine to produce methylhippuric

and hippuric acid, then excreted in the urine [8].

The photophysical stability as well as chemical reactivity of

toluic acid isomers is the primary concern and could be altered

by an alternative approach: Mr. Trivedi's biofield energy

treatment. The properties could be enhanced by utilizing The

Trivedi Effect®, a unique biofield energy treatment by Mr.

Trivedi which is alre ady known to alter the physical and

structural properties of various living and non-living

substances [9,10]. The National Center for Complementary

and Alternative Medicine (NCCAM) has recommended the

use of biofield therap y as a part of complementary and

alternative medical therapies (CAM) in the healthcare sector

[11]. CAM includes numerous energy-healing therapies, in

which the biofield therap y is a for m of putative energy

medicine that is being widely used world wide to improve the

overall health of human beings. Humans have the ability to

harness energy from the environment/universe and can be

transmitted to any object (living or non-livin g) around the

globe. This process is called biofield energy treatment. The

impact of the Trivedi Effect® has been studied in various

agricultural research [9,10,12-14]. Based on the outstanding

results achieved by the biofield energy treatments on ceramics,

microbiology, and agricultural research, an attempt was made

to evaluate the effect of biofield energy treatment on the

isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM in

2.1. Materials and Methods

Both, o-toluic acid (OTA) and m-toluic acid (MTA) were

procured from S D Fine Chemicals Pvt. Limited, India. Each

of the samples was distributed into two parts, where one part

of each sample was referred as control sample and the other

part was considered as treated. The treated sample was further

divided into four groups (i.e. T1, T2, T3, and T4) and handed

over to Trivedi for biofield energy treatment under standard

laboratory conditions. Trivedi provided the treatment through

his energy transmission process to the treated group without

touching the sample. T he control and treated samples were

characterized using gas chromatography-mass spectrometry

Elmer(USA)/auto system XL with Turbo mass, having

detection limit up to 1 picogram. The GC-MS spectra were

obtained in the form of % abundance vs. mass to charge ratio

(m/z). The iso topic abundance ratio 2H/1H or 13C/12C

(PM+1)/PM and 18O/16O (PM+2)/PM was expr essed by its

deviation in treated samples as compared to the control one.

The percentage change in isotopic ratio (PM+1)/PM and

(PM+2)/PM were calculated with the following formula:

Percent change in isotopic abundance ratio

Where, RTreated and RControl are the ratios of intensity at

(PM+1) to PM in mass spectra of treated and control samples,

3. Results and Discussion

Figure 1. GC-MS spectrum of control o-toluic acid sample.

The GC-MS spectra of control and treated (T1, T2, T3, and

T4) samples are presented in Figure 1 and 2, respecti vely.

Mass spectra of OTA is well matched with the reported

literature [15]. MS spectra showed five peaks including

molecular ion peak (PM) for both the control and treated

samples of OTA. Five major peaks at m/z 136, 118, 91, 65, and

39 were observed that corresponded to the following ions

ions. Peak at m/z 136 was the molecular ion peak and the other

peaks observed in bo th, control and all treated OTA samples

were due to the fragmentation of OTA inside mass spectrum.

American Journal of Applied Chemistry 2015; 3(6): 217-223 219

The base peak at m/z 91 was observed due to b reaking of

o-toluic acid to stable toluene molecule that showed the

highest intensity in the mass spectrum. Further, m/z 118, 65,

and 39 peaks were observed due to first reduction of the

molecular ion to aldehyde, which again fragmented to giv e

cyclopentene and propyne respectively. The intensity ratio and

calculated percentage isotopic abundance ratio of (PM+1)/PM

and (PM+2)/PM is presented in the Table 1. Moreover, the

isotopic abundance ratio (PM+1)/PM and (PM+2)/PM in

control and treated OTA was plotted in Figure 3.

Table 1. GC-MS isotopic abundance analysis result of o-toluic acid.

Peak Intensity Control Treated

Figure 2. GC-MS spectra of treated o-toluic acid samples T1, T2, T3, and T4.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Gunin Saikia Snehasis Jana

# data[].attributes.attachment_link.title
Quantitative Determination of Isotopic Abundance Ratio of 13C, 2H, and 18O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers - PdfSR.com

# data[].attributes.attachment_link.description
O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic intermediates, mostly used in medicines and pesticides. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, (PM+1)/PM and 18O/16O, (PM+2)/PM, in toluic acid isomers using gas chromatography-mass spectrometry (GC-MS).