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King George III drew a line on a map over the Appalachian mountains and said something along the lines of..
"I do declare that from this day hence though shan't go forth and pass east of thy mountains, I do declare." (He just came back from a trip to the south, well in his dreams)

This got the colonists mad since they just fought a war for this land, and now they wanted to colonize it.

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The British finally decided to enforce the previous taxes that they had placed. This caused the colonists to be upset since they actually had to pay taxes now, ending the period of 'Salutary Neglect.'

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1763 should be considered a turning point since after the events of the French and Indian war the British were like, "Yo we need to tax these fools" so they started to enforce their taxes to pay off their War Debt from both the Seven Years War. But after years of freedom from these taxes, the colonists were fueled with resentment towards the British since they suddenly started to tax common goods, and they were no longer able to trade with other nations (Navigation Acts). This with the fact that the colonists believed the British weren't allowing them go east of the Appalachian mountains since they had just fought for this land against the French. This caused tensions to be at a high point between the colonists and the Brits.