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Sarah Tovar Flores

Period 3

3/15/22

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DNA stands for Deoxyribonucleic Acid and is made up of nucleotides. Nucleotides are made up of phosphate, sugar and nitrogen base. The base pair rules are A=T and G=C. Bases are connected by special chemical bonds called hydrogen bonds. DNA looks like a backbone/sides of ladders that are made of phosphates and sugars.

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heavy duty ziploc bag
1 strawberry
goggles
10 mL DNA extraction buffer (soapy, salty water)
Cheesecloth
Funnel
20 mL test tube & test tube stand
Stir stick
10 mL ethanol (FREEZER)
Paper towels

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Step 1: Place strawberry inside the ziploc bag
Step 2: Smash up the strawberry
Step 3: Add the10 mL of extraction buffer (salt & soap solution) to the bag
Step 4: Mush the strawberry in the bag again for 1 minute
Step 5: Assemble your filtration apparatus as shown in the diagram
Step 6: Pour the strawberry slurry into the filtration apparatus & let it drip directly into your test tube up until your test tube is ½ full
Step 7: Slowly pour cold ethanol into the tube
Step 8:Dip the stir stick into the tube where the strawberry extract and ethanol layers come into contact with each other

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I smashed the strawberry to break open the cells. Afterwards, I mushed the strawberry with salty/soapy solution to break up proteins & dissolve cell membranes. Next, I filtered the strawberry slurry through cheesecloth to break up proteins & dissolve cell membranes. Lastly, I added an addition of ethanol to the filtered extract so that the DNA could precipitate from the solution.

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What we did in this lab is smash the strawberry in the ziploc bag, then poured it into a tube and from then poured ethanol to extract the strawberry's DNA. What I learned about DNA is that it is not invisible to the naked eye but if gathered together it becomes visible. What I would do differently in this experiment if given another chance is breaking down the strawberry a bit more. After extracting the DNA I would observe the DNA and throw away stuff that isn't needed anymore. Two traits that are expressed in a strawberry are its size and color.