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		XRD & TGA-DTG Analysis of Methyl 2-Naphthyl Ether
	

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		XRD & TGA-DTG Analysis of Methyl 2-Naphthyl Ether
	

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		Methyl 2-Naphthyl Ether Characterization & Energy Treatment
	

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		Methyl 2-Naphthyl Ether Characterization & Energy Treatment
	

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Environmental Analytical Chemistry

Trivedi et al., J Environ Anal Chem 2015, 2:5

http://dx.doi.org/10.4172/2380-2391.1000162

Research ArticleOpen Access

Volume 2 * Issue 5 * 1000162

ISSN: 2380-2391 JREAC, an open access journal

Keywords: Methyl-2-naphthyl ether; Bioeld energy; X-ray

diraction; Surface area analysis; Dierential scanning calorimetry;

ermogravimetric analysis

MNE: Methyl-2-Naphthyl Ether; NCCAM: National Center For

Complementary And Alternative Medicine; XRD: X-Ray Diraction;

DSC: Dierential Scanning Calorimetry; TGA: ermogravimetric

Analysis; DTA: Dierential ermal Analysis; DTG: Derivative

ermogravimetry; FT-IR: Fourier Transforms Infrared

Naphthalene has been described as new class of potent

antimicrobials against wide range of human pathogens. It occupies a

central place among biologically active compounds owing to its varied

and exciting antibiotic properties with less toxicity [1]. Numerous

naphthalene containing antimicrobial drugs are existing like naine,

nafacillin, terbinane, tolnaate, etc. that plays vital role against

microbial infections [2,3]. Further, the naphthalene derivatives like

naproxen, nabumetone were also studied in depth as the nonsteroidal

anti-inammatory drugs (NSAIDs). ey are mainly nonselective

inhibitor of two cyclooxygenase (COX) isoform i.e., COX-1 and COX-2

[4,5]. Moreover, the structurally relative naphthalene derivatives lower

the parathyroid level by binding to calcium receptor on the parathyroid

gland. us, it helps to regulate hyperparathyroidism especially in

kidney disease or parathyroid gland neoplasm [6]. Based on the

importance of naphthalene derivative as a main moiety for organic

synthesis of several pharmaceutical drugs, it is advantageous to nd

out the alternate approach that can enhance the physicochemical and

thermal properties of naphthalene derivative i.e., methyl-2-naphthyl

ether (MNE). Recently, an alternate treatment approach i.e.,healing

therapy or therapeutic touch, known as the bioeld energy treatment,

which was reported in several elds. e National Institute of Health/

National Center for Complementary and Alternative Medicine (NIH/

NCCAM) conceived the bioeld energy treatment in subcategory of

energy therapies (putative energy elds) [7,8]. e bioeld treatment is

Physical, Thermal, and Spectroscopic Characterization of Biofield Energy

Treated Methyl-2-Naphthyl Ether

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Khemraj Bairwa2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

being used in healing process to reduce pain, anxiety and to promote

the overall health of human being [9,10]. Bioeld is an electromagnetic

eld that permeates and surrounds living organisms. is biologically

produced electromagnetic and subtle energy eld regulates the various

physiological and communications functions within the human

organism [11]. Researchers have attempted dierent biological studies

and eects of bioeld on various biomolecules such as proteins,

antibiotics [12], bacterial cultures [13], and conformational change in

DNA [14]. Prakash et al. reported that various scientic instruments

such as Kirlian photography, resonance eld imaging (RFI) and

polycontrast interference photography (PIP) could be extensively used

to measure the bioeld of human body [15]. us, the human has the

ability to harness the energy from the environment or Universe and

transmit it to any living or nonliving object on the Globe. e object(s)

receive the energy and respond into the useful way; this process is termed

as bioeld treatment. Mr. Trivedi's unique bioeld energy treatment is

known as e Trivedi Eect®. Recently, bioeld energy treatment has

been described as an alternative method to alter the physicochemical

and thermal properties of several metals and ceramics [16-18]. It

has also reported to alter the spectroscopic properties of various

pharmaceutical drugs like paracetamol, piroxicam, metronidazole, and

tinidazole [19,20]. Moreover, the bioeld treatment has been studied in

Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of

several antimicrobial and anti-inammatory agents. This study was attempted to evaluate the impact of bioeld energy

treatment on the physical, thermal, and spectroscopic properties of MNE. The study was carried out in two groups i.e.,

samples of MNE were evaluated using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry

(DSC), thermogravimetric analysis-derivative thermogravimetric analysis (TGA-DTG), Fourier transform infrared (FT-

IR), and ultraviolet-visible (UV-Vis) spectroscopy. The XRD study exhibited the decrease in average crystallite size by

30.70%. The surface area analysis showed 5.32% decrease in surface area of the treated sample with respect to the

control. The DSC thermogram of treated MNE exhibited no signicant change in the melting temperature; however,

the latent heat of fusion was slightly increased (0.83%) after bioeld treatment as compared to the control sample.

The TGA analysis showed the onset temperature of thermal degradation at 158℃ in the control sample that was

reduced to 124℃ after bioeld treatment. The result showed about 21.52% decrease in onset temperature of thermal

degradation of treated MNE as compared to the control. Similarly, the end-set temperature of thermal degradation was

also reduced by 13.51% after bioeld treatment with respect to the control. The FT-IR and UV spectroscopic studies

did not show any changes in the wavenumber and wavelength, respectively in treated MNE with respect to the control.

Overall, the XRD, surface area and thermal analysis suggest that bioeld treatment has the impact on physical and

thermal properties of the treated MNE as compared to the control.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad

Rd., Bhopal-462026 Madhya Pradesh, India, Tel: 91-755-6660006; E-mail:

Received September 07, 2015; AcceptedSeptember14, 2015; Published

Citation:Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, et al.(2015)

Physical, Thermal, and Spectroscopic Characterization of Bioeld Energy

Treated Methyl-2-Naphthyl Ether. J Environ Anal Chem 2: 162. doi:10.4172/2380-

Copyright: © 2015 Trivedi MK , et al . This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Volume 2 * Issue 5 * 1000162

ISSN: 2380-2391 JREAC, an open access journal

Treated Methyl-2-Naphthyl Ether. J Environ Anal Chem 2: 162. doi:10.4172/2380-2391.1000162

a heating rate of 5ºC/min under air atmosphere. e onset temperature

of thermal degradation and percent change intemperature at which

maximum weight loss occur in samples were obtained from DTG

For the purpose of FT-IR and UV-Vis spectroscopic

characterization, the treated sample was divided into two groups i.e., T1

and T2. Both the treated groups were then analyzed for spectroscopic

characteristics employing FT-IR and UV-Vis spectroscopy and data

were compared with the respective spectrum of control sample.

FT-IR spectroscopic characterization

e samples for FT-IR spectroscopy were prepared by crushing

into ne powder and then mixing in spectroscopic grade KBr. Finally,

the mixture was pressed into pellets with a hydraulic press and then

used for analysis. e spectra were recorded from Shimadzu's Fourier

transform infrared spectrometer (Japan) with the frequency range of

4000-500 cm-1. e analysis was done to evaluate the impact of bioeld

energy treatment at the atomic levels like force constant, dipole

moment, and bond strength in chemical structure [27].

Uv-Vis spectroscopic analysis

e samples solutions for UV spectroscopy were prepared in

methanol. UV spectra of the control and treated samples of MNE were

acquired from Shimadzu UV-2400 PC series spectrophotometer with

quartz cuvette having slit widths of 2.0 nm. e wavelength was set

between 200-400 nm for analysis. e study was carried out to evaluate

the impact of bioeld energy treatment on the energy gap of highest

occupied molecular orbital and lowest unoccupied molecular orbital

XRD diractograms of both control and treated MNE are shown

in Figure 1. e control sample exhibited the XRD peaks at 2θ equal

to 10.27º, 15.44º, 19.07º, 20.57º, 21.21º, 24.76º, 25.33º, and 28.77º.

Likewise, the XRD diractogram of treated MNE showed the XRD

peaks at 2θ equal to 10.25º, 15.30º, 19.01º, 20.55º, 21.17º, 24.67º, 25.25º,

and 28.84º. e intensity of peak was also altered aer bioeld energy

treatment as compared to the control. XRD diractograms of both the

control and treated MNE showed the intense peaks, which suggest the

crystalline nature of MNE. e decrease of XRD peaks intensity in

treated sample might be attributed to the reduction in crystallinity and

decrease in long-range order of the molecules. e crystallite size was

calculated using Scherrer formula and the results are shown in Figure

2. e average crystallite size of the control MNE was observed as 84.55

nm that was decreased up to 58.59 nm in the treated sample. e result

indicated about 30.70% decrease in average crystallite size of treated

sample with respect to the control.

e increase in internal micro strain leads to decrease the

corresponding crystallite size of the material [28]. Moreover, Zhang et al.

reported that presence of strain and increased atomic displacement from

their ideal lattice positions lead to reduction in crystallite size [29]. Hence,

it is assumed that bioeld energy treatment probably induce the internal

strain in treated MNE sample. is might be responsible for the decrease

in crystallite size of the treated MNE as compared to the control.

e surface area of control and treated samples of MNE were

several elds like agriculture research [21,22], biotechnology research

[23], and microbiology research [24,25].

Based on the published literature and outstanding impact of

bioeld energy treatment on various living and nonliving things, the

present study was aimed to evaluate the impact of Mr. Trivedi's bioeld

energy treatment on physical, thermal and spectroscopic properties of

MNE using several analytical techniques.

e Methyl-2-Naphthyl Etherwas procured from Sisco Research

Laboratories, India. e study was performed in two groups i.e.,

control and treated. e control sample was remained as untreated,

and the treated group in sealed pack was handed over to Mr. Trivedi

for bioeld energy treatment under laboratory conditions. Mr.

Trivedi provided the bioeld energy treatment to the treated group

through his unique energy transmission process without touching the

sample. Subsequently, the control and treated samples of MNE were

analyzed using several analytical techniques like X-ray diraction

(XRD), surface area analysis, dierential scanning calorimetry (DSC),

thermogravimetric analysis (TGA), Fourier transform infrared (FT-

IR), and ultraviolet-visible (UV-Vis) spectroscopy.

e XRD analysis of the control and treated MNE was carried out

on Phillips, Holland PW 1710 X-ray diractometer with nickel lter

and copper anode. e wavelength used in XRD system was 1.54056 Å.

e XRD diractograms were obtained in the form of a chart of 2θ vs.

intensity. e crystallite size (G) and percent change in crystallite size

of MNE were calculated using the following equations [26].

Crystallite size (G) = kλ/(bCosθ)

Where, Gc and Gt are average crystallite size of control and treated

powder samples respectively.

e surface area of the control and treated MNE was measured

using the Brunauer-Emmett-Teller (BET) surface area analyzer, Smart

SORB 90. Percent change in surface area was calculated using following

%change in surface area=×100

Where, S Control and S Treated are the surface area of the control and

treated samples, respectively.

e control and treated samples of MNE were studied using a

Pyris-6 Perkin Elmer dierential scanning calorimeter on a heating

rate of 10ºC/min under air atmosphere with air ow rate of 5 mL/

min. An empty pan sealed with cover was used as a reference pan. e

melting temperature (Tm ) and latent heat of fusion (ΔH) were obtained

TGA-DTG analysis was conducted to investigate the thermal

stability of the control and treated MNE. e studies were carried out

on Mettler Toledo simultaneous TGA-DTG system. Both the control

and treated samples were heated from room temperature to 400ºC with

Volume 2 * Issue 5 * 1000162

ISSN: 2380-2391 JREAC, an open access journal

Treated Methyl-2-Naphthyl Ether. J Environ Anal Chem 2: 162. doi:10.4172/2380-2391.1000162

determined using BET surface area analyzer and data are presented

in Figure 3. e surface area of the control and treated sample was

observed as 0.4904 m2 /g and 0.4643 m2/g, respectively. e result

showed a considerable decrease in surface area i.e., 5.32% in the treated

MNE as compared to the control sample. It is assumed that bioeld

energy treatment possibly induced the disappearance of inter-particle

boundaries that may lead to the aggregation of particles and thus

increase in particle size [30]. Presumably, this increase in particle size

might lead to decrease in surface area of the treated sample.

DSC analysis was performed to determine the melting temperature

and latent heat of fusion (ΔH) of the control and treated MNE. DSC

thermograms (Figure 4) of MNE showed the melting temperature at

73.72ºC in the control and 73.66ºC in the treated sample (Table 1). e

result depicted no signicant change in melting temperature of treated

sample as compared to the control. e melting temperature of control

MNE was well supported by literature data [31]. DSC thermogram

exhibited the ΔHof 147.49 J/g in control sample and 148.72 J/g in

treated sample of MNE. e result showed about 0.83% increase in

latent heat of fusion of treated sample as compared to the control. It

is hypothesized that bioeld energy treatment may cause absorption

of energy during the phase transition from solid to liquid that might

lead to increase the latent heat of fusion of treated sample with respect

to the control. Previously, our group has reported that bioeld energy

treatment altered the value of latent heat of fusion in lead and tin

e TGA and DTG thermograms of control and treated samples of

MNE are shown in Figure 5 and data are reported in Table 1. e onset

temperature of thermal degradation was observed at 158°C and 124°C

for the control and treated samples, respectively. Whereas, the end-set

temperature of thermal degradation was found at 201°C and 178°C in

the control and treated sample, respectively. e result showed about

21.52% decrease in the onset temperature and 11.44% decrease in the

end-set temperature in treated sample with respect to the control. e

percent weight loss during thermal decomposition was 63.41% in the

control and 59.18% in the treated sample. It showed the decrease in

% weight loss during thermal decomposition aer bioeld treatment.

Based on this, it is presumed that bioeld treated MNE may be more

stable as compared to the control. e DTG thermogram exhibited the

Tmax (the temperature at which the sample lost its maximum weight) at

177.81°C in the control sample and at 153.78°C in the treated sample

of MNE. e result revealed about 13.51% decrease in Tmax of treated

sample with respect to the control. is decrease in Tmax in treated

sample might be due to increase in vaporization or volatilization [32]

of treated MNE molecules aer bioeld energy treatment. It might

be correlated with the alteration in internal energy through bioeld

energy treatment that results into earlier vaporization in treated sample

as compared to the control.

FT-IR spectroscopic analysis

FT-IR spectra of the control and treated samples of MNE (Figure

6) were inferred using the theoretically predicted wavenumber.e

aromatic =C-H stretching peak was assigned at 3007-3057 cm-1 in all

three samples i.e., the control and treated (T1 and T2). Likewise, the

-C-H (methyl) stretching was attributed to peak at 2962 cm-1 in all

three samples i.e., the control and treated (T1 and T2). e aromatic

C=C stretching of naphthyl moiety was appeared in the region of 1508-

Figure 1: XRD diffractograms of methyl-2-naphthyl ether.

Figure 2: Crystallite size of control and treated methyl-2-naphthyl ether.

Figure 3: Surface area analysis of control and treatedmethyl-2-naphthyl ether.

# data[].attributes.attachment_link.title
Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Methyl-2-Naphthyl Ether - PdfSR.com

# data[].attributes.attachment_link.description
Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of several antimicrobial and anti-inflammatory agents. This study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of MNE. The study was carried out in two groups i.e., control and treated.

# data[].attributes.headline
	
	
	
	




	
	
		Alterations in Methyl 2-Naphthyl Ether Physical Properties
	

# data[].attributes.subject
	
	
	
	




	
	
		Alterations in Methyl 2-Naphthyl Ether Physical Properties
	

# data[].attributes.body
| The XRD and surface area analysis data highlights the alterations in Methyl 2-Naphthyl Ether physical properties as a result of unique energy medicine.

# data[].attributes.attachment_link.keywords[]
methyl 2-naphthyl ether physical properties

# data[].attributes.attachment_link.title
"Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Methyl-2-Naphthyl Ether" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of several antimicrobial and anti-inflammatory agents. This study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of MNE. The study was carried out in two groups i.e., control and treated.

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		TGA Analysis of Methyl 2-Naphthyl Ether
	

# data[].attributes.subject
	
	
	
	




	
	
		TGA Analysis of Methyl 2-Naphthyl Ether
	

# data[].attributes.body
| TGA analysis of methyl 2-naphthyl was conducted to investigate the thermal stability of treated MNE after unique human energy treatment. Follow the link to get the more information.

# data[].attributes.headline
	
	
	
	




	
	
		Biofield Impact on Methyl 2-Naphthyl Ether Characterization
	

# data[].attributes.subject
	
	
	
	




	
	
		Biofield Impact on Methyl 2-Naphthyl Ether Characterization
	

# data[].attributes.body
| The present work shows the effects of biofield energy treatment on the physical, thermal, and spectroscopic characterization of Methyl-2-Naphthyl Ether. Visit to get more information.

# data[].attributes.attachment_link.title
Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Methyl-2-Naphthyl Ether

# data[].attributes.attachment_link.description
Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of several antimicrobial and anti-inflammatory agents. This study was attempted to evaluate the impact of biofield energy treatment on the physical,

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		Energy Medicine & Spectroscopic Properties of MNE
	

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		Energy Medicine & Spectroscopic Properties of MNE
	

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| Read here how energy treatment has altered the spectroscopic properties of MNE. The unique energy treatment has also resulted in changes of physical and thermal characteristics of MNE.

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		Methyl 2-Naphthyl Ether – Impact of Human Energy
	

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		Methyl 2-Naphthyl Ether – Impact of Human Energy
	

# data[].attributes.body
| This study was attempted to evaluate the impact of human energy treatment on Methyl 2-Naphthyl Ether. Read here for more details.

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Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Methyl-2-Naphthyl Ether (PDF Download Available)

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Full-text (PDF) | Methyl-2-naphthyl ether (MNE) is an organic compound and used as the primary moiety for the synthesis of several antimicrobial and anti-inflam...

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		Trivedi Effect Research | Researchgate
	

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		Trivedi Effect Research | Researchgate
	

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| Here you can find the research papers where the concept of The Trivedi Effect is used for bringing the positive changes on the tested organic or inorganic compounds and materials. Visit for the more information

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		Know about the Trivedi Effect of Google Plus
	

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		Know about the Trivedi Effect of Google Plus
	

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		Trivedi Effect | Energy Transformation
	

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		Trivedi Effect | Energy Transformation
	

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| Mahendra Trivedi is a founder of the Trivedi Effect & Trivedi Foundation. He has the unique abilities to harness the energy from the universe and transmit it to anywhere on earth.

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Trivedi Effect - Free downloads and reviews - CNET Download.com

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trivedi effect free download - Advanced Effect Maker Freeware Edition, Photoshop Manga Effect Plugin (32-bit), Paint.NET, and many more programs

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		Spectroscopic Test of 1,2,3-Trimethoxybenzene
	

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		Spectroscopic Test of 1,2,3-Trimethoxybenzene
	

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| Spectroscopical tests of 1,2,3- Trimethoxybenzene was conducted in this research using the FT-IR, UV-Vis analysis. Read here for more details.

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Volume 4 * Issue 4 * 1000136

ISSN: 2329-6631 JDD an open access journal

Research ArticleOpen Access

Journal of Developing Drugs

Trivedi et al.,J Develop Drugs 2015, 4:4

http://dx.doi.org/10.4172/2329-6631.1000136

Keywords: X-ray diraction; ermal analysis; Fourier transform

infrared (FT-IR) spectroscopy; Ultra violet-visible spectroscopy (UV-

Abbreviations: XRD: X-ray diraction; DSC: Dierential scanning

calorimetry; TGA: ermogravimetric analysis; FT-IR: Fourier

transform infrared; UV-Vis: Ultra Violet-Visible spectroscopy analysis;

CAM: Complementary and alternative medicine

Benzene-based compounds are used as an intermediate for

the synthesis of pesticides and other chemicals. Methoxybenzene

derivatives such as anisole (methoxybenzene) and veratrole

(1,2-dimethoxybenzene) are widely used in perfumes, insect

pheromones, pharmaceuticals [1] and synthesis of organic compounds

[2]. 1,2,3-Trimethoxybenzene is an organic compound with excellent

physical properties due to its interesting chemical structure [3]. It has

been used to study the eect of solvent on photo-induced electron

transfer reactions [4]. 1,2,3-Trimethoxybenzene was used as an

intermediate for the synthesis of 2,4-diamino-5-benzylpyrimidines

and analogues for antimicrobial applications [5]. However,

1,2,3-trimethoxybenzene based compounds are potentially toxic in

nature and causes enormous health problems. ese compounds are

found in many hazardous waste sites that have been proposed for

inclusion in the environmental protection agency (EPA) national

priority list [6]. e distribution and degradation of the chemicals

entirely depend on physicochemical properties of the chemicals. ese

properties are namely molecular weight, solubility, volatilization,

and polarity [7,8]. It was reported that by improving volatilization

of the chemicals it might accelerate the degradation by reacting with

photochemicaly-produced free radicals [9]. Chakraborty et al. used

anaerobic degradation of benzene derivatives by Dechloromonas

strain [10]. However, these methods are not cost eective. us,

some alternate strategies should be designed which can increase the

degradation of 1,2,3-Trimethoxybenzene, ultimately it might reduce

the health problems associated with this compound. Recently, bioeld

energy treatment was used as potential strategy to alter the physical,

chemical and thermal properties of metals [11,12], ceramic [13], organic

compounds [14,15], and organic products [16,17]. erefore, authors

planned to investigate the inuence of bioeld energy treatment on

physical, thermal and spectral properties of 1,2,3-trimethoxybenzene.

e National Centre for Complementary and Alternative Medicine

(NCCAM), which is a part of the National Institute of Health (NIH),

endorses the use of Complementary and Alternative Medicine (CAM)

therapies as an alternative in the healthcare sector, and about 36%

of Americans regularly uses some form of CAM [18]. CAM includes

numerous energy-healing therapies; bioeld therapy is one of the

energy medicine used worldwide to improve overall health.

Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and

pharmaceutical agents. The objective of this study was to investigate the inuence of bioeld energy treatment on the

physical, thermal and spectral properties of 1,2,3-trimethoxybenzene.

Methods: The study was performed by dividing the sample into two groups (control and treated). The control

group remained as untreated, while the treated group received Mr Trivedi's bioeld energy treatment. The control

and treated 1,2,3-trimethoxybenzene samples were then characterized by X-ray diffraction (XRD), differential scanning

calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-

visible spectroscopy (UV-Vis) analysis.

Results: XRD studies revealed the signicant increase in crystallite size of treated sample by 45.96% as compared

to the control sample. DSC analysis showed a decrease in melting temperature of the treated sample (45.93ºC) with

respect to control (46.58ºC). Additionally, the substantial change was evidenced in latent heat of fusion of treated

sample by 64.18% as compared to the control. TGA analysis indicated a decrease in maximum thermal decomposition

temperature (Tmax ) of treated sample (151.92ºC) as compared to the control sample (154.43ºC). This indicated the

decrease in thermal stability of the treated sample as compared to the control. FT-IR spectroscopic analysis showed

an increase in the frequency of C-O bond in treated sample (1105→1174 cm-1 ) as compared to the control sample.

However, UV analysis showed no changes in absorption peaks in treated sample as compared to the untreated sample.

Conclusion: Overall, the result indicated that bioeld energy treatment has altered the physical, thermal and

spectral properties of the treated sample as compared to control. Hence, the treated sample could be used as an

intermediate in the synthesis of organic compounds.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462

Received September 16, 2015; Accepted September 29, 2015; Published

Citation: Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015)

Physical, Thermal and Spectral Properties of Bioeld Treated 1,2,3-Trimethoxybenzene.

J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits un-

restricted use, distribution, and reproduction in any medium, provided the original

author and source are credited.

Physical, Thermal and Spectral Properties of Biofield Treated

Mahendra Kumar Trivedi1, Rama Mohan Tallapragada1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Rakesh Kumar Mishra2 and

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya Pradesh, India

Citation:Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Physical, Thermal and Spectral Properties of Bioeld Treated

1,2,3-Trimethoxybenzene. J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Volume 4 * Issue 4 * 1000135

ISSN: 2329-6631 JDD an open access journal

Researchers have shown that when atoms undergo any

translation, vibration, rotation and quantiable motion a measurable

electromagnetic radiation is generated which is evident by acoustic

or vibrational spectroscopy [19]. Likewise, the cells present in the

human body are consisting of the electron, proton, neutron and

fundamental particles that always remain in a state of vibratory motion

[19]. Additionally, neurons that are present in the human central

nervous system have the ability to transmit the information in the

form of electrical signals [20-23]. us, human bioeld is referred

as an energetic eld or matrix that surrounds the human body. is

energetic eld is identical to superhighway that allows DNA in our

cells to communicate faster than light and maintain coherent, holistic

intelligence in the organism [24]. erefore, it is envisaged that human

beings have the ability to harness the energy from the environment/

Universe and can transmit into any object (living or non-living)

around the Globe. e object(s) will always receive the energy and

responding in a useful manner that is called bioeld energy. Mr Trivedi

is known transform the characteristics of various living and non-living

things using his unique bioeld energy. is bioeld energy treatment

is also known as e Trivedi Eect®. It is known to alter phenotype

characteristics of microbes [25,26] and improved the growth and

anatomical characteristics of medicinal plants [27,28].

Aer considering the potential of bioeld energy treatment and

chemical properties of 1,2,3- trimethoxybenzene, this study was

conducted to investigate the inuence of this treatment on physical,

thermal and spectral properties of the compound.

1,2,3-Trimethoxybenzene was procured from S D Fine Chemicals

Ltd., India. e sample was divided into two parts; one was kept as a

control sample while the other was subjected to Mr Trivedi's unique

bioeld energy treatment and coded as treated sample. e treated

group was in sealed pack and handed over to Mr Trivedi for bioeld

energy treatment under standard laboratory conditions. Mr Trivedi has

given the energy treatment through his energy transmission process

to the treated samples without touching the sample. e control and

treated samples were characterized by X-ray diraction, Dierential

scanning calorimetry, ermogravimetric analysis, Fourier transform

infrared spectroscopy, and Ultra violet-visible analysis.

X-ray diraction (XRD) study: e XRD analysis of control

and treated 1,2,3-trimethoxybenzene were carried out using Phillips,

Holland PW 1710 X-ray diractometer system, which contains a

copper anode with nickel lter. e radiation of wavelength used by the

XRD system was 1.54056 Å. e XRD results obtained were presented

in the form of a chart of 2θ vs. intensity. e average crystallite size (G)

was calculated by using formula:

Here, λ is the wavelength of radiation used; b is full-width half-

maximum (FWHM) of peaks and k is the equipment constant (=0.94).

Percentage change in average crystallite size was calculated using

Percent change in average crystallite size=[(Gt -Gc)/Gc ] × 100

Where, Gc and Gt are the average crystallite size of control and

treated powder samples respectively.

Dierential scanning calorimetry (DSC): e control and treated

1,2,3-trimethoxybenzene samples were analysed using a Pyris-6 Perkin

Elmer DSC at a heating rate of 10ºC/min under air atmosphere, and

the air was ushed at a ow rate of 5 mL/min. Predetermined amount

of sample was kept in an aluminium pan and closed with a lid. A blank

aluminium pan was used as a reference. e percentage change in

latent heat of fusion was calculated using following equations:

% change in Latent heat of fusion 100

Where, ΔH Control and ΔH Treated are the latent heat of fusion of

control and treated samples, respectively.

ermogravimetric analysis-Dierential thermal analysis

(TGA-DTA): e thermal stability of control and treated

1,2,3-trimethoxybenzene were analysed by using Mettler Toledo

simultaneous TGA and Dierential thermal analyser (DTA). e

samples were heated from room temperature to 400ºC using a heating

rate of 5ºC/min under air atmosphere.

FT-IR spectroscopy: e FT-IR spectra were recorded on

Shimadzu's Fourier transform infrared spectrometer (Japan) with the

frequency range of 4000-500 cm-1 . e analysis was accomplished to

evaluate the eect of bioeld treatment at an atomic level like dipole

moment, force constant and bond strength in chemical structure [29].

e treated sample was divided into two parts T1 and T2 for FT-IR

UV-Vis spectroscopic analysis: UV spectra of the control and

treated 1,2,3-trimethoxybenzene samples were acquired on Shimadzu

UV-2400 PC series spectrophotometer with 1 cm quartz cell and a

slit width of 2.0 nm. Methanol was used as a solvent and the analysis

was carried out using wavelength in the range of 200-400 nm. e UV

spectra were analysed to determine the eect of bioeld treatment

on the energy gap of highest occupied molecular orbital and lowest

unoccupied molecular orbital (HOMO-LUMO gap) [29]. e treated

sample was divided in two parts T1 and T2 for the analysis.

e XRD was used to investigate the crystalline nature of control

and treated 1,2,3-trimethoxybenzene sample. e XRD diractogram

of control and treated 1,2,3-trimethoxybenzene are presented in Figure

1. XRD diractogram of control 1,2,3-trimethoxybenzene showed

intense crystalline peaks at 2θ equal to 13.05º, 13.29º, 13.54º, 19.54º,

21.67º, 22.75º, 25.55º, 25.77º, 26.78º and 27.20º. Whereas, the treated

1,2,3-trimethoxybenzene also showed XRD peaks at 2θ equal to 13.50º,

13.68º, 19.24º, 22.99º, 26.03º, 27.09º and 27.31º. e comparative

evaluation of the XRD diractograms showed the decrease in

intensity of XRD peaks of treated samples with respect to the control.

It is assumed that bioeld energy treatment might disturb the long-

range pattern of the 1,2,3-trimethoxybenzene molecules that led to a

reduction in crystallinity of the sample.

e crystallite size of control and treated 1,2,3-trimethoxybenzene

was computed using Scherrer formula, and data are depicted in

Figure 2. e crystallite size is known as a group of molecules or

atoms having orientation in the same plane. It was reported that

crystallite size directly inuences the materials properties, and it is

one of the crystallographic parameters linked with the formation of

dislocations and point defects in a crystal structure [30]. e crystallite

size of control 1,2,3- trimethoxybenzene was 67.23 nm, and it was

substantially increased up to 98.13 nm in the treated sample. e result

indicated 45.96% increase in the crystallite size of treated compound

as compared to the control sample. Pang and Bao reported that due

Citation:Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al. (2015) Physical, Thermal and Spectral Properties of Bioeld Treated

1,2,3-Trimethoxybenzene. J Develop Drugs 4: 136. doi:10.4172/2329-6631.1000136

Volume 4 * Issue 4 * 1000135

ISSN: 2329-6631 JDD an open access journal

to synthesis temperature and ripening on hydroxyapatite causes an

increase in crystallite size [31]. Rey et al. [32] and Drouet et al. [33]

reported an increase in crystal size with increase in temperature and

pH. Hence, it is presumed here that bioeld treatment might provide

thermal energy to the treated 1,2,3-trimethoxybenzene atoms or

molecules that lead to a reduction in dislocation density and increase

in crystallite size as compared to the control. Carballo et al. reported

that the rate of reaction can be signicantly improved by an increase

in crystallite size [34]. us, it is assumed that the increased crystallite

size of treated 1,2,3-trimethoxybenzene might accelerate its reaction

rate and percentage reaction yield during the synthesis of compounds.

Additionally, the increased crystallite size can be correlated with

increase in particle size and hence decrease solubility [35]. Hence, it

is assumed that this might increase the volatilization leading to faster

degradation of the treated sample as compared to control.

DSC study: DSC was used to investigate the latent heat of fusion and

melting nature of the control and treated samples. DSC thermogram

of control and treated 1,2,3-trimethoxybenzene are presented in

Figure 3. e DSC thermogram of control 1,2,3-trimethoxybenzene

showed a sharp endothermic inexion at 46.58ºC that was due

to melting temperature of the sample. However, the treated

1,2,3-trimethoxybenzene showed an endothermic peak at 45.93ºC.

is indicated the decrease in melting temperature of the treated

sample with respect to the control. It was reported that if the molecules

contributing to a lattice are rigid, compact and symmetrical they tend

to maximize the intermolecular interactions. us, a compound with

less compact molecular arrangement has a low melting temperature

than compactly arranged compound i.e., more energy is needed to

separate the molecules [36]. Hence, it is assumed that bioeld treated

sample might have a less compact arrangement that lead to the decrease

e latent heat of fusion was computed from the DSC thermogram

and data are presented in Table 1. e control compound showed a latent

heat of fusion of 104.28 J/g and it was increased signicantly to 171.21

J/g in the treated sample. e results indicated a signicant increase in

latent heat of fusion by 64.18% as compared to the control sample. e

latent heat of fusion is considered as the energy required to overcome

Figure 1: XRD diffractograms of control and treated 1,2,3-trimethoxybenzene.

Figure 3: DSC thermograms of control and treated 1,2,3-trimethoxybenzene.

Figure 2: Crystallite size of control and treated 1,2,3-trimethoxybenzene.

ParameterControl Treated

Latent heat of fusion ΔH (J/g)104.28171.21

Melting temperature (ºC)46.5845.93

Weight loss (%)53.9357.04

Table 1: Thermal analysis data of control and treated 1,2,3-trimethoxybenzene.

Tmax : Maximum thermal decomposition temperature.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene - PdfSR.com

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 1,2,3-trimethoxybenzene. Methods: The study was performed by dividing the sample into two groups (control and treated).

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		1,2,3 Trimethoxybenzene- Impact of Spiritual Energy
	

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		1,2,3 Trimethoxybenzene- Impact of Spiritual Energy
	

# data[].attributes.body
| Want to know how the physical, thermal and spectral properties of 1,2,3-Trimethoxybenzene can be altered? Visit here to get information about the science used to alter the properties and results.    | http://works.bepress.com/mahendra_trivedi/66/

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fourier transform infrared (ft-ir) spectroscopy

# data[].attributes.attachment_link.keywords[]
spectral properties of 1 2 3-trimethoxybenzene

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of 1,2,3-trimethoxybenzene. Methods: The study was performed by dividing the sample into two groups (control and treated).

# data[].attributes.headline
	
	
	
	




	
	
		DSC Analysis of 1,2,3 Trimethoxybenzene
	

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		DSC Analysis of 1,2,3 Trimethoxybenzene
	

# data[].attributes.body
| The study shows,  after the DSC analysis of human energy treated 1,2,3 Trimethoxybenzene, a significant alteration in melting temperature was recorded.

# data[].attributes.headline
	
	
	
	




	
	
		Alteration of Spectral Properties of 1,2,3-Trimethoxybenzene
	

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		Alteration of Spectral Properties of 1,2,3-Trimethoxybenzene
	

# data[].attributes.body
| The study speculates that human energy might be altered the spectral properties of 1,2,3-trimethoxybenzene by signiﬁcantly increases the latent heat.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Treated 1,2,3-Trimethoxybenzene

# data[].attributes.attachment_link.description
Study background: 1,2,3-Trimethoxybenzene is an important compound used for the synthesis of chemicals and pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and

# data[].attributes.headline
	
	
	
	




	
	
		Evaluation of 1,2,3-Trimethoxybenzene Properties
	

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		Evaluation of 1,2,3-Trimethoxybenzene Properties
	

# data[].attributes.body
| The study shows the alteration of 1,2,3 Trimethoxybenzene properties in physical, thermal and spectral aspects. For more details visit the link.

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		Human Energy Treatment of 1,2,3 Trimethoxybenzene
	

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		Human Energy Treatment of 1,2,3 Trimethoxybenzene
	

# data[].attributes.body
| The main objective of this study is to investigate the influence of unique human energy on the physical & thermal properties of 1,2,3-trimethoxybenzene.

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		Mahendra Kumar Trivedi - Master of Transformation
	

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		Mahendra Kumar Trivedi - Master of Transformation
	

# data[].attributes.body
| Mahendra Kumar Trivedi is the founder of Trivedi Foundation & Trivedi Science. He is also known as a Master of transformation because of his unique ability to use biofield energy treatment for the wellness of human being.

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		About Mr. Mahendra Kumar Trivedi
	

# data[].attributes.subject
	
	
	
	




	
	
		About Mr. Mahendra Kumar Trivedi
	

# data[].attributes.body
| Mr. Mahendra Kumar Trivedi has the unique ability to harness the energy from the universe and transmit it to anywhere on earth, infusing it into living and non-living materials to optimize their potential. Read here for the more details.

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		Mahendra Kumar Trivedi | Biography
	

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		Mahendra Kumar Trivedi | Biography
	

# data[].attributes.body
| Read here the biography of Mahendra Kumar Trivedi. He is known for his unique biofield energy called "The Trivedi Effect".

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Mahendra Kumar Trivedi | Madhya Pradesh University

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Mahendra Kumar Trivedi,Madhya Pradesh University,Advances in Automobile Engineering .

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		Mahendra Trivedi's Impact on P-Chloro Benzonitrile
	

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		Mahendra Trivedi's Impact on P-Chloro Benzonitrile
	

# data[].attributes.body
| P-Chloro Benzonitrile DSC analysis shows the change in melting temperature (50 deg.c to 350 deg.c) & latent heat of fusion. For more details visit the link.

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Science Journal of Chemistry

Published online October 15, 2015 (http://www.sciencepublishinggroup.com/j/sjc)

doi: 10.11648/j.sjc.20150306.11

ISSN: 2330-0981 (Print); ISSN: 2330-099X (Online)

Physical, Thermal and Spectroscopic Studies of Biofield

Treated p-Chlorobenzonitrile

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal, Madhya

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana. Physical, Thermal and Spectroscopic

Studies of Biofield Treated p-chlorobenzonitrile. Science Journal of Chemistry. Vol. 3, No. 6, 2015, pp. 84-90.

doi: 10.11648/j.sjc.20150306.11

Para-chlorobenzonitrile (p-CBN) is widely used as a chemical inter mediate in the manufacturing of dyes,

medicines, and pesticides, however; sometimes it may cause runaway reaction s at high temperatures. The current study was

designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

The analysis was d one by dividing the p-CBN samples into two groups that served as control and treated. The treated group

received Mr. Trivedi's biofield treatment. Subsequently, the control and treated samples were evaluated using various

analytical techniques such as X-ra y diffraction (XRD), surface area analyser, differential scanning calorimetry (DSC),

thermogravimetric a nalysis (T GA), Fourier-tr ansform infra red (FT-IR) and UV-visible (UV-Vis) spectroscopy. The XRD

results showed an increase in the crystallite size (66.18 nm) of the treated sample as compared to the control sample (53.63

nm). The surface area analysis of the treated sample also showed 14.19% decrease in the surface area as compared to control.

Furthermore, DSC analysis results showed that the latent heat of fusion of the treated p-CBN increased considerably by 5.94%

as compared to control. However, the melting temperature of the treated sample did not show any considerable change from

the control sample. Besides, TGA/DTG studies showed that T

(the temperature at which the sample lost its maximum

weight) was increased by 5.22% along with a n increase in its onset of thermal d ecomposition temperature i.e. 9 6.80°C in the

biofield treated p-CBN as compared to the control sample (84.65°C). This indicates that the thermal stability of treated p-CBN

sample might increase as compared to the control sample. Ho wever, no change was found in the FT-IR and UV-Vis

spectroscopic character of the tr eated p-CBN as compared to the control. These findings suggest that the biofield treatment

Biofield Energy Treatment, Para-Chlorobenzonitrile, X-ray Diffraction Study, Surface Area Analyzer,

Differential Scanning Calorimetry, Thermogravimetric Analysis

Aromatic nitriles have wide applications in the production

of dyes, pesticides and pharmaceuticals. They are used as

intermediates in the synthesis of various pharmacologically

relaxants, neuroleptics, etc. [1].They are also widely used in

the synthesis of HIV-1 non-nucleoside reverse transcriptase

inhibitors and the arylation of oxazoles and poly ether amides

[2-4]. Benzonitriles are of great interest in the field of organic

benzonitriles are al so used in the synthesis of various drugs

like chlorazepate, climazolam and loflazepate, etc. [5]. Many

derivatives of benzonitrile are used in salt form as urinary

antiseptics and in vapour form for disinfecting bronchial

arylation on benzimidazoles [6].

The stability profile of any chemical compound is the most

desired quality that determines its shelf life and purity to be

used as an intermediate. The stability can be correlated to the

physical, thermal or structural and bonding properties of a

compound [7]. Currently, the stability of chemical compounds

in pharmaceutical industries can be affected due to altering

temperatures and pH conditions [8]. Moreover, many reactions

in industries were carried out at high temperatures. Hence, the

chemical compounds. The thermal stability is also considered

in the processing, long-term storage or shipping of material.

When a chemical has high stability, it is more resistant to

breaking down or decomposition. The higher stability also

helps to avoid runaway reactions [9]. However, benzonitriles

are reported to cause runaway reactions during chlorination

and cyanation reactions [10, 11]. Thus, it is important to search

for an alternate strategy that can improve the stability of

chemical compounds by altering their physical, thermal or

structural and bonding properties.

In recent years, biofield energy has been known for its

impact on various living organisms and non-living materials.

A biofield is the electromagnetic field that permeates and

surrounds living organisms, and the energy associated with

this field is kno wn as biofield energy [12, 13]. According to

existing theories, this biofield constitutes a dynamic living

communicating information to a nd throughout the body. It is

believed that any imbalance in this biofield energy leads t o

disease. The health of living organisms can be affected by

balancing this energy from the environment through a natural

popular in holistic medicine health care systems, and are

included in the National Centre for Complementary and

National Institute o f Health ( NIH). NCCAM p laces biofield

therapy (putative energy fields) as the subcategory o f energy

medicine among complementary and alternative medic ines.

[16, 17]. These healing treatments suggest their mechanism

upon modulating patient-environmental energy fields. T hus,

the human has the ability to harness the energy from the

environment or universe and can transmit it to any living

organism or non-living object. This process is termed as

biofield treatment. Mr. Trivedi is well-known to possess a

unique biofield energy tr eatment (The Trivedi Effect

has been significantly studi ed in different fields such as

microbiology research [18-20], agriculture research [21,22],

and biotechnology research [23,24]. Recently, it was reported

that biofield treatment has changed the atomic, crystalline

alteration in ph ysical, thermal and chemical p roperties were

also reported in materials like aluminium and ceramic oxide

[27, 28]. Hence, based on above results the curre nt study was

designed to determine the impact of biofield treatment on

physical, thermal and spectral properties of p-CBN.

p-chlorobenzonitrile (p-CBN) was procured from S D Fine

Chemicals Pvt. Ltd., India. The sample was divided into two

parts; one was kept as the control while other was subjected

control and treated samples were further characterizations

using XRD, surface area analyser, DSC, TGA, FT-IR and

UV-Vis spectroscopic techniques.

2.1. Biofield Treatment Modalities

evaluating the impact of biofield treatment. The first set of

the sample was considered as control, and no treatment was

given to this part. The second set of t he sample was handed

over to Mr. Trivedi for biofield energy treatment under

standard laboratory conditions. Mr. Trivedi provided the

biofield treatment through his energy transmission process,

which includes bioenergy emission for 3-5 min. without

touching the sample. After treatment, the sample was handed

over in the same condition and stored at standard conditions

as per the sta ndard experimental protocol. The optimum

experiments. The differences in parameters before and after

the treatment were noted and compared.

2.2. X-ray Diffraction (XRD) Study

XRD analysis was carried out on Phillips, Holland PW

1710 X-ray diffractometer system. The X-ray generator was

equipped with a copper anode with nickel filter operating at

35kV and 20mA. The wavelength of radiation used by the

acquired over the 2θ range o f 10°-99.99° at 0.02° interval

with a measurement time of 0.5 seconds per 2θ intervals. T he

data obtained were in the form of a chart of 2θ vs. intensity

and a detailed table containing peak intensity counts, d val ue

), and relative intensity (%).

The average size of crystallite (G) was calculated from the

Scherrer equation (1) with the method based on the width of

Where, k is the equipment constant (0.94), λ is the X-ray

wavelength (0.154 nm), B in radians is the full-width at half

of the peaks and θ the corresponding Bragg angle.

Percent change in crystallite size was calculated using the

Percent change in crystallite size = [(G

are crystallite size o f control and treated

powder samples respectively.

2.3. Surface Area Analysis

analyser, Smart SORB 90 based on Br unauer-Emmett-Teller

(BET). Percent changes in surface area were calculated using

Science Journal of Chemistry 2015; 3(6): 84-90 86

 

are the surface area of control

and treated samples, respectively.

2.4. Differential Scanning Calorimetry (DSC) Study

temperature and latent hea t of fusion (∆ H). The DSC curve s

heating rate of 10°C/min in the temperature range of 50°C to

350°C. An empty pan sealed with cover pan was used as a

reference sample. Melting temperature and latent heat of

fusion were obtained from the DSC curve.

Percent change in latent heat of fusion was calculated

using following equations (4) to observe the difference in

thermal properties of treated p-CBN sample as compared to

 !!!""

are the latent heat of fusion

of control and treated samples, respectively.

2.5. Thermogravimetric Analysis/ Derivative

Thermogravimetry (TGA/DTG)

Thermal stability of control and treated sample of p-CBN

thermogravimetric analyser (TGA/DTG). The samples were

heated from room temperature to 400ºC with a heating rate of

(temperature at which sample start losing

weight) and from DTG curve, T

sample lost its maximum weight) were recorded.

Percent change intemperature at which maximum weight

loss occur in sample was calculated usi ng following equation

which maximum weight loss occurs in c ontrol and treated

Percent change inonset peak temperature was calculated

using following equation (6):

% change in onset peak temperature, T

temperature in control and treated sample, respectively.

2.6. Fourier Transform-Infrared (FT-IR) Spectroscopic

The samples were crushed into fine powder for anal ysis.

The powdered sample was mixed in spectroscopic grade KBr

in an agate mortar and pressed into pellets with a hydraulic

press. FT-IR spectra were recorded on Shimadzu's Fourier

transform infrared spectrometer (Japan). FT-IR spectra are

generated by the ab sorption of electromagnetic radiation in

the frequency range 4000-400 cm

. The FT-IR spectroscopic

analysis of p-CBN was carried out to evaluate the impact of

biofield treatment at atomic and molecular level like bond

strength, stability, rigidity of structure, etc. [29].

2.7. UV-Vis Spectroscopic Analysis

wavelength range of 200-400 nm with 1 cm quartz cell and a

slit width of 2.0 nm. This analysis was performed to evaluate

the effect of biofield trea tment on the structural property of

p-CBN sample. With UV-Vis spectroscopy, it is possible to

investigate electron transfers between orbitals or bands of

atoms, ions and molecules existing in the gaseous, liquid and

3. Results and Discussion

X-ray diffraction analysis was conducted to study the

crystalline nature of the control and treated sample of p-

CBN. XRD diffractogram of control and treated samples of

p-CBN are shown in Figure 1. The XRD diffractogram of

control p-CBN showed intense crystalline peaks at 2θ equal

to 1 4.11°, 15.27°, 19.61°, 25.26° and 28.39°. The intense

peaks indicated the crystalline nature of p-CBN. However,

crystalline peak at 2θ equal to 14.05°, 15.27°, 1 9.55°, 25.16°,

and 28.34°. Most of the treate d sample peaks showed similar

crystallinity of treated p-CBN sample was not changed as

compared to control. Besides, the crystallite size was found

respectively. The crystallite size was increased b y 23.4% i n

previously reported that cyano group has donor-acceptor

dimensional network of molecules [30]. It is presumed that

molecules that may lead to the formation of a supra molecular

structure of p-CBN due to the interaction of C≡ N---Cl. This

formation of supramolecular structure may lead to increase in

crystallite size of p-CBN after biofield treatment. T his type

of interactio n was also reported by D. Britton in 2-

chlorobenzonitriles, which l ead to the formation of four

crystallographic non-equivalent for ms of molecules [31]. As

the intermolecular bonding leads to stability of the

compound, hence it suggests that biofield treatment might

enhance the stability of treated p-CBN.

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ragini Singh, Snehasis Jana

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile - PdfSR.com

# data[].attributes.attachment_link.description
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

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		Spectroscopic Analysis of P-Chloro Benzonitrile: Impact of Human Energy
	

# data[].attributes.subject
	
	
	
	




	
	
		Spectroscopic Analysis of P-Chloro Benzonitrile: Impact of Human Energy
	

# data[].attributes.body
| The current studies of para chloro benzonitrile is designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic property.

# data[].attributes.attachment_link.keywords[]
thermal studies of para chloro benzonitrile

# data[].attributes.attachment_link.keywords[]
spectroscopic studies of p-chloro benzonitrile

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the impact of biofield energy treatment on the physical, thermal, and spectroscopic properties of p-CBN.

# data[].attributes.headline
	
	
	
	




	
	
		P-Chloro Benzonitrile DSC Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		P-Chloro Benzonitrile DSC Analysis
	

# data[].attributes.body
| According to p-Chloro Benzonitrile DSC analysis, there is an increase in latent heat of fusion by 5.94% as compared to control after biofield. For more details visit the link.

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		Thermal Studies of Para Chloro Benzonitrile: Biofield Impact
	

# data[].attributes.subject
	
	
	
	




	
	
		Thermal Studies of Para Chloro Benzonitrile: Biofield Impact
	

# data[].attributes.body
| Thermal study of Para Chloro Benzonitrile was carried out using TGA/DTG analysis methods to evaluate the impact of biofield energy on the p-CBN. Visit the link to read the complete paper and results.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectroscopic Studies of Biofield Treated p-Chlorobenzonitrile

# data[].attributes.attachment_link.description
Para-chlorobenzonitrile (p-CBN) is widely used as a chemical intermediate in the manufacturing of dyes, medicines, and pesticides, however; sometimes it may cause runaway reactions at high temperatures. The current study was designed to evaluate the

# data[].attributes.headline
	
	
	
	




	
	
		P-Chloro Benzonitrile XRD Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		P-Chloro Benzonitrile XRD Analysis
	

# data[].attributes.body
| P-Chloro Benzonitrile XRD Analysis was carried out to evaluate changes in its physical properties due to the application of unique human energy treatment.

# data[].attributes.headline
	
	
	
	




	
	
		Studies of Para Chloro Benzonitrile Characteristics
	

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		Studies of Para Chloro Benzonitrile Characteristics
	

# data[].attributes.body
| Read here about the effects of unique energy medicine on the physical and structural characteristics of Para Chloro Benzonitrile.

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		Mahendra Trivedi's Research on Antimicrobial Sensitivity of P. Fluorescens
	

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		Mahendra Trivedi's Research on Antimicrobial Sensitivity of P. Fluorescens
	

# data[].attributes.body
| The study shows that Mahendra Trivedi's Biofield Energy treatment has made a significant alteration in antimicrobial sensitivity pattern of P. Fluorescens.

# data[].attributes.attachment_link.title
Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment

# data[].attributes.attachment_link.description
Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this

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		Mahendra Trivedi on iScience
	

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		Mahendra Trivedi on iScience
	

# data[].attributes.body
| Mahendra Trivedi unconventional approach called Biofield energy treatment has the ability of transformation. Read more about the unique abilities of Mahendra Trivedi on iScience.

# data[].attributes.attachment_link.title
Scientists and Researchers Network - I am in Science - Member Profile - Mahendra Trivedi

# data[].attributes.attachment_link.description
Scientists' and researchers' network for interaction and content sharing. I am in Science. This is a member's profile.

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		Mahendra Trivedi - Founder of The Trivedi Effect®
	

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		Mahendra Trivedi - Founder of The Trivedi Effect®
	

# data[].attributes.body
| The "Trivedi Effect®" founded by Mr. Mahendra Trivedi, is a world-renowned, natural phenomenon of transforming living organisms and non-living materials to perform at the highest level.

# data[].attributes.attachment_link.description
The Trivedi Effect - Trivedi Effect - The One Energy That can Transform Any Thing - View company info, team members, fundraising and more

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		Dr. Deepak Chopra about Mahendra Trivedi
	

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		Dr. Deepak Chopra about Mahendra Trivedi
	

# data[].attributes.attachment_link.title
Dr. Deepak Chopra about Mahendra Trivedi

# data[].attributes.attachment_link.description
Dr. Deepak Chopra is reviewing on Mahendra Trivedi. Dr. Chopra says that the interview i am going to do now it is the one of the most unusual interview i have ever done in my life i have very unusual guest ( Mahendra Kumar Trivedi) this evening .Is Mahendra Trivedi a guru?

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		Biofield Impact on Biotype Number of R. Ornithinolytica
	

# data[].attributes.subject
	
	
	
	




	
	
		Biofield Impact on Biotype Number of R. Ornithinolytica
	

# data[].attributes.body
| The experimental result shows the change in biotype number of biofield treated R. Ornithinolytica from (77754370) at day 10 with respect to untreated samples (77755372). For more results view the link.

# data[].attributes.attachment_link.keywords[]
susceptibility pattern of r. ornithinolytica

# data[].attributes.attachment_link.title
"Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
	
	
	
	




	
	
		Improved Susceptibility Pattern of Raoultella Ornithinolytica
	

# data[].attributes.subject
	
	
	
	




	
	
		Improved Susceptibility Pattern of Raoultella Ornithinolytica
	

# data[].attributes.body
| Read here about the impact of a new approach known as the biofield energy treatment and its impact on improving the susceptibility pattern of R. Ornithinolytica.

# data[].attributes.headline
	
	
	
	




	
	
		Evaluation of Raoultella Ornithinolytica Antimicrobial Susceptibility
	

# data[].attributes.subject
	
	
	
	




	
	
		Evaluation of Raoultella Ornithinolytica Antimicrobial Susceptibility
	

# data[].attributes.body
| Biofield  treatment  could  be  used  as  complementary  and alternative treatment strategy against the multidrug-resistant strain of  R. Ornithinolytica in order to improve the antimicrobial sensitivity and reduce the MIC values.

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so present study aimed to evaluate the impact of Mr. Trivedi's biofield treatment on antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical study, and biotype number of multidrug resistant strain of R.

# data[].attributes.headline
	
	
	
	




	
	
		Change in Biotype Number of R. Ornithinolytica
	

# data[].attributes.subject
	
	
	
	




	
	
		Change in Biotype Number of R. Ornithinolytica
	

# data[].attributes.body
| The study suggests an alteration in biotype number of R. Ornithinolytica after the influence of biofield treatment. For more details visit the link.

# data[].attributes.attachment_link.title
Biofield Treatment: An Alternative Approach to Combat Multidrug-Resistant Susceptibility Pattern of Raoultella ornithinolytica

# data[].attributes.attachment_link.description
Raoultella ornithinolytica is belongs to the family of Enterobacteriaceae, a Gram-negative encapsulated aerobic bacillus associated with bacteremia and urinary tract infections. As biofield therapy is increasingly popular in biomedical heath care, so

# data[].attributes.headline
	
	
	
	




	
	
		Alteration in R. Ornithinolytica Biochemical Characteristics
	

# data[].attributes.subject
	
	
	
	




	
	
		Alteration in R. Ornithinolytica Biochemical Characteristics
	

# data[].attributes.body
| The study suggests the change in R. Ornithinolytica biochemical characteristics after biochemical treatment. Read here for more details.

# data[].attributes.headline
	
	
	
	




	
	
		Impact of Biofield in Multidrug Resistant of Raoultella Ornithinolytica
	

# data[].attributes.subject
	
	
	
	




	
	
		Impact of Biofield in Multidrug Resistant of Raoultella Ornithinolytica
	

# data[].attributes.body
| The recent study is based on the concept of unique energy treatment by Mr. Mahendra Trivedi. The impact of the same was eveluated on the multidrug resistant of Raoultella Ornithinolytica. Follow the link to get more information.

# data[].attributes.headline
	
	
	
	




	
	
		The Trivedi Effect - A New Scientific Approach
	

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		The Trivedi Effect - A New Scientific Approach
	

# data[].attributes.body
| The Trivedi Effect has proved impact on not only on the human wellness and health disorders but also in scientific researches and experiments, conducted in different fields of science.

# data[].attributes.attachment_link.description
The Trivedi Effect has proved its impact in not only human wellness and health disorders but also in scientific researches and experiments.

# data[].attributes.headline
	
	
	
	




	
	
		The Trivedi Effect | Characterization of Chromium (Vi) Oxide
	

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		The Trivedi Effect | Characterization of Chromium (Vi) Oxide
	

# data[].attributes.body
| The presentation posted here shows the importance of The Trivedi Effect for altering the characteristics of Chromium (Vi) Oxide.

# data[].attributes.attachment_link.description
Mahendra Kumar Trivedi earned his 5-Year Bachelor's degree in Mechanical Engineering in 1985. Mr. Mahendra Kumar Trivedi worked as an Engineer for 10 Years. In 1995, Mr. Trivedi discovered that he had the unique ability to harness the energy from the universe and transmit it to anywhere on the globe, infusing it into living organisms and nonliving materials, thus optimizing their potential.

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		Unique Energy Treatment | The Trivedi Effect
	

# data[].attributes.subject
	
	
	
	




	
	
		Unique Energy Treatment | The Trivedi Effect
	

# data[].attributes.body
| The Trivedi Effect is a natural phenomenon that transforms living organism and non-living materials through the energy transmissions by Mahendra Trivedi.

# data[].attributes.attachment_link.description
The Trivedi Effect®is a unique phenomenon that changes life forms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters™. This helpful...

# data[].attributes.headline
	
	
	
	




	
	
		The Trivedi Effect by Mr. Mahendra Kumar Trivedi
	

# data[].attributes.subject
	
	
	
	




	
	
		The Trivedi Effect by Mr. Mahendra Kumar Trivedi
	

# data[].attributes.body
| Many people have reported that the phenomenon of The Trivedi Effect has transformed their relationships, dramatically increased their happiness and helped them discover their life’s true purpose. This phenomenon is also used positively in hundreds of research papers.

# data[].attributes.attachment_link.readable_text
The Trivedi Effect® is a natural phenomenon that transforms living organisms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters ™. This beneficial transformation is accomplished by strengthening their connection to the source of all creation - Universal Intelligence, enabling them to function at a higher level and serve a greater purpose.

The phenomenon of the 21st Century

The world today is witnessing the emergence of an extraordinary phenomenon that offers a radical way to transform life on this planet for the benefit of all. This phenomenon - brought to the world by Mahendra Trivedi - is known as The Trivedi Effect®.

Mahendra Trivedi - and The Trivedi Masters ™, Dahryn Trivedi, Gopal Nayak and Alice Branton - possess the ability to create The Trivedi Effect® through the Energy Transmissions generated by their thoughts.

Benefits of The Trivedi Effect

The Trivedi Effect ® has the ability to transform all living organisms, including plants, seeds, crops, bacteria, viruses, fungi, animals, cancer cells, and human cells by strengthening their connection to the Universal Energy which is the source of all creation. It is the ONE thing that CAN transform ANY thing.

Tens of thousands of people throughout the world have experienced this phenomenon and are now enjoying the journey of their life.

Astounding the scientific community

The Trivedi Effect® has surprised and astounded the worldwide scientific community. Results from scientific experiments and the vastly growing body of human testimonials have challenged current scientific understanding, pushing scientific thought into new lines of enquiry. Scientific research has shown that The Trivedi Effect® is a natural phenomenon not yet understood by science and is unparalleled in the world as we know it. It is non-invasive in nature and beneficial to all life.

Extensive scientific research

The Trivedi Effect ® has been explored in a number of different subject areas, including agriculture, genetics, cancer, microbiology, material science and animal husbandry through the collaborative efforts of leading scientists from all over the world and the Trivedi Foundation™.

The impact of this phenomenon has been measured, validated and documented in over 4,000 studies at major research institutes throughout the world. Further, these empirical studies have resulted in the publication of numerous substantiating articles in leading international peer-reviewed journals.

Scientific studies performed on non-human and non-living materials subjected to Energy Transmissions have shown dramatic results, demonstrating the fact that the effects of this energy are real and not occurring as a result of a placebo effect. There is no doubt that The Trivedi Effect® has substantial power to change the capabilities of almost everything on this planet.

The Trivedi Effect has:

Increased the yield of crops by up to 500 percent and their immunity by up to 600 percent without the use of any chemicals, fertilizers or pesticides

Identified and killed, in a rigorous, U.S.-based laboratory setting, cancer cells while simultaneously sustaining and promoting greater viability in healthy cells

Reduced the viral load of HIV, Hepatitis B and Hepatitis C, in vitro by up to 99.8 percent

Strengthened the composition of metals and other non-living materials at the atomic level

The Trivedi Effect ® has also brought about health improvements as reported by tens of thousands of individuals - including those with chronic pain, debilitating arthritis, food sensitivities, heart disease, cancer, depression, low self-esteem and more.

Many people have even reported that The Trivedi Effect® has transformed their relationships, dramatically increased their happiness and helped them discover their life's true purpose.

Some benefits are experienced immediately, while others unfold over time. No matter how big or small the benefits seem to be, people everywhere have experienced remarkable and even life-changing results.

If The Trivedi Effect® can do all this, imagine what it could do for you!

Brain MRI

Thermal Imaging

Spine MRI

" The Augmentation and Aortic reflection show the amount of stiffness seen in a person 20-25 years younger"
-Dr. John Levin, Anti-Aging Specialist, Australia

" The pituitary fossa is the largest ever found in a healthy subject with fully functioning body having excellent health"
-Dr Shailendra Patil, India

# data[].attributes.attachment_link.title
The Trivedi Effect® | Mahendra Trivedi | Human Anatomy and Physiology

# data[].attributes.attachment_link.description
The Trivedi Effect ® is a natural phenomenon that transforms living organisms and non-living materials through the Energy Transmissions of Mahendra Trivedi and The Trivedi Masters ™. This beneficial transformation is accomplished by strengthening their connection to the source of all creation - Universal Intelligence, enabling them to function at a higher level and serve a greater purpose.

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		Citrobacter Braakii – Study of Biochemical Characteristics
	

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		Citrobacter Braakii – Study of Biochemical Characteristics
	

# data[].attributes.body
| Unique healing therapy has significantly affected the biochemical characteristics of Citrobacter Braakii.  Read more the detailed information about the changes brought by the unique healing therapy.

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		16s rDNA Sequencing of Human Energy Treated C. Braakii
	

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		16s rDNA Sequencing of Human Energy Treated C. Braakii
	

# data[].attributes.body
| 16S rDNA analysis of Human Energy Treated C. Braakii showed that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state. Visit for more information.

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

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		Study of Biochemical Characteristics of Citrobacter Braakii
	

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		Study of Biochemical Characteristics of Citrobacter Braakii
	

# data[].attributes.body
| C. braakii has possessed a high level of resistance to the penicillin and other antibiotics. Therefore, the present study was conducted to evaluate the impact of alternative energy therapy on biochemical characteristics of Citrobacter Braakii.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Research ArticleOpen Access

Trivedi et al., J Clin Med Genom 2015, 3:1

http://dx.doi.org/10.4172/jcmg.1000129

Research ArticleOpen Access

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

Journal of Clinical & Medical Genomics

Keywords: Citrobacter braakii; Antimicrobial susceptibility; Bioeld

treatment; Biochemical reaction; Biotype; 16S rDNA analysis; Gram-

negative bacteria; Enterobacteriaceae

Abbreviations: MDR: Multi-Drug Resistant; ATCC: American Type

Minimum Inhibitory Concentration;OTUs: Operational Taxonomic

Units;NCBI: National Center for Biotechnology Information;MEGA:

Molecular Evolutionary Genetics Analysis; PCR: Polymerase Chain

Reaction; RDP: Ribosomal Database Project; HBMEC: Human Brain

Microvascular Endothelial Cells

Citrobacter braakii (C. braakii) is a genus of Gram-negative, straight,

facultative anaerobic and motile bacilli bacterium widely distributed in

water, soil, and food in the environment. It is also commonly found in

urinary, intestinal, and respiratory tract of human and animals, belongs

to Enterobacteriaceae family. It has been associated with various

nosocomial and community acquired infections in humans [1]. Arens

et al. reported about 11 genetically distinct species within the genus

Citrobacter [2]. e main clinical manifestations have been reported

due to nosocomial infections of Citrobacter species such as bacteremias

[3], endocarditis [4], urinary tract infections [1], neonatal meningitis

[5], pneumonia [6] and brain abscess [7]. Based on literature it has

been demonstrated that 0.8% of Gram-negative infections caused by

causes nosocomial infection among all Enterobacteriaceae family

[9]. Although it has low virulence property responsible for host cell

invasion instead of it invade blood brain barrier (BBB) of human brain

microvascular endothelial cells (HBMEC) and causes meningitis.

Moreover, due to overproduction of chromosomal β-lactamase

enzyme leads to antimicrobials resistance [10]. Aminoglycosides,

uoroquinolones, carbapenems, new oral cephems and many third and

fourth-generation cephems, such as cefepime and cefpirome, are the

drugs of choice to treat C. braakii associated infections but it possess

high level of resistance against penicillin and other antibiotics[11,12].

erefore, an alternative strategy is needed to alter the antimicrobial

sensitivity prole against C. braakii strain. In recent years, bioeld

treatment was proved to be an alternative method which has impact on

various properties of living and non-living materials in a cost eective

manner. It is already demonstrated that energy can neither be created

nor be destroyed but it can be transferred through various processes such

as thermal, chemical, kinetic, nuclear, etc. [13-15]. Similarly, electrical

current exists inside the human body in the form of vibratory energy

particles like ions, protons, and electrons and they generate magnetic

eld in the human body [16,17]. Aerward, Harold Saxton Burr had

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research

Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad

Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91 755 666 0006; E-mail:

Received August 20, 2015; Accepted September 23, 2015; Published September

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, et al. (2015)

braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study

was attempted to investigate the effect of Mr. Trivedi's bioeld treatment on C. braakii in lyophilized as well as

revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized

vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was further

subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysed

on day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes.

First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters were

studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample

was carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobial

susceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treated

cells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant to

susceptible after bioeld treatment, with support of decreased MIC value (>8 to ≤ 4 µg/mL) by two-fold in all the

treated samples as compared to the control. Biochemical reactions also showed signicant (42.42%) alteration in

the treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA

(53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus)

on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with species

were substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and

(53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showed

that the identied microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. The

results suggested that bioeld treatment has a signicant impact on C. braakii in lyophilized as well as revived state.

Phenotyping and 16S rDNA Analysis after Biofield Treatment on

1Trivedi Global Inc., Eastern Avenue Suite A-969, Henderson, NV, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Chinar Fortune City, Hoshangabad Rd., Madhya Pradesh, India

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

performed the detailed studies on the correlation of electric current

with physiological process and concluded that every single process in

the human body had an electrical signicance [18]. Recently, it was

discovered that all electrical process happening in body have strong

relationship with magnetic eld as mentioned by Ampere's law, which

states that the moving charge produces magnetic elds in surrounding

space [19,20]. us, the human body emits the electromagnetic waves

in form of bio-photons, which surround the body and it is commonly

known as bioeld. erefore, the bioeld consists of electromagnetic

eld, being generated by moving electrically charged particles (ions,

cell, molecule, etc.) inside the human body. According to Rivera-Ruiz

et al., it was reported that electrocardiography has been extensively

used to measure the bioeld of human body [21]. us, human has the

ability to harness the energy from environment or universe and can

transmit into any living or nonliving object(s) around the Globe. e

objects always receive the energy and responding into useful way that

is called bioeld energy and the process is known as bioeld treatment.

Mr. Trivedi's unique bioeld treatment (e Trivedi eect®) has been

known to transform the structural, physical and thermal properties of

several metals and ceramic in materials science [22-24], improved the

overall productivity of crops [25,26], altered characteristics features of

microbes [27-29] and improved growth and anatomical characteristics

of medicinal plants [30,31].

Due to the clinical signicance of this organism and literature on

bioeld treatment, the present work was undertaken to evaluate the

impact of bioeld treatment on C. braakii in relation to antimicrobials

susceptibility and biotyping based on various biochemical characters

followed by 16S rDNA sequencing analysis.

C. braakii, American Type Culture Collection (ATCC 43162) strain

was procured from MicroBioLogics, Inc., USA and stored with proper

storage conditions until further use. All the tested antimicrobials and

biochemicals were procured from Sigma-Aldrich (MA, USA). e

antimicrobial susceptibility, biochemical reactions and biotype number

were estimated with the help of MicroScan Walk-Away® (Dade Behring

Inc., West Sacramento, CA, USA) using Negative Breakpoint Combo

30 (NBPC 30) panel with respect to control group (Gr.). e 16S rDNA

sequencing study was carried out using ultrapure genomic DNA prep

kit; Cat KT 83 (Bangalore Genei, India).

e impact of bioeld treatment on tested bacterium C. braakii was

Group I: ATCC strain was revived from lyophilized state and

considered as control. No treatment was given and analyzed for

antimicrobial sensitivity, biochemical reactions and biotype number as

per the standard protocol.

two parts named as Gr. IIA and Gr. IIB. Both the groups of ATCC

strain of C. braakii in lyophilized state were assigned to the Mr. Trivedi's

unique bioeld treatment (rst treatment). Gr. IIB sample was stored in

lyophilized state for 159 days at -70ºC. Gr. IIBwas further sub-divided

in two separate parts named as Gr. IIB - Study Iand Gr. IIB - Study II.

Aer 159 days, antimicrobial sensitivity, MIC, biochemical

reactions and biotyping were performed as per the standard protocol.

e stored strain was revived from -70ºC and the revived culture

was again provided to Mr. Trivedi's bioeld treatment (re-treatment)

on day 159. Aer bioeld retreatment, the sample was sub-cultured

into three separate tubes on 3 dierent days (Day 0, Day 5 and Day

10) and analysed keeping the main treated tube aside. Each sample was

analyzed aer 5 days of its sub-culturing.

Bioeld treatment strategy

e lyophilized (Gr. IIA) sample of C. braakii was subjected to Mr.

Trivedi's bioeld treatment (rst treatment) followed by retreatment

aer storing for 159 days in revived state (Gr. IIB, Study II). In details,

the treatment groups in sealed pack were handed over to Mr. Trivedi for

bioeld treatment under laboratory conditions. Mr. Trivedi provided

the treatment through his energy transmission process to the treated

groups without touching the samples. Aer rst treatment, the analysis

of Gr. IIA lyophilized sample was done on day 10 for antimicrobial

sensitivity along with minimum inhibitory concentration (MIC),

biochemical reactions with biotype number and 16S rDNA analysis as

per the standard protocol. While handing over these cultures to Mr.

Trivedi for retreatment purposes, optimum precautions were taken to

Antimicrobial susceptibility test

Investigation of antimicrobial susceptibility of C. braakii was

carried out with the help of automated instrument, MicroScan Walk-

Away® using NBPC 30 panel. e panel can be stored at 2 to 25ºC for

analysis. e panel was allowed to equilibrate to room temperature

prior to rehydration. All opened panels were used on the same day.

e tests carried out on MicroScan were miniaturized of the broth

dilution susceptibility test that has been dehydrated. Briey, 0.1 mL

of the standardized suspension of C. braakii was pipetted into 25

mL of inoculum water using pluronic and inverted 8 to 10 times and

inoculated, rehydrated, and then subjected to incubation for 16 hours at

35°C. Rehydration and inoculation was performed using the RENOK®

system with inoculators-D (B1013-4). 25 mL of standardized inoculum

suspension was poured in to inoculum tray. e detailed experimental

procedure and conditions were followed as per the manufacturer's

instructions. e antimicrobial susceptibility pattern (S: Susceptible,

R: Resistant; and I: Intermediate) and MIC values were determined by

observing the lowest antimicrobial concentration showing inhibition

Biochemical reaction studies

Biochemical reactions of C. braakii were determined using

MicroScan Walk-Away®, system with NBPC 30 panel. Preparation of

NBPC 30 panel, inoculum followed by dehydration and rehydration

were performed in a similar way as mentioned in antimicrobial

susceptibility assay for analysis of biochemical reactions followed by

biotype number. e detailed experimental procedures and conditions

were followed as per the manufacturer's instructions [32].

Identication of organism by biotype number

e biotype number of C. braakii was determined on MicroScan

Walk-Away® processed panel data report with the help of biochemical

Amplication and gene sequencing of 16S rDNA

Genomic DNA was isolated from C. braakii cells (Gr. IIA, sample coded

Citation: Trivedi MK, Branton A, Trivedi D , Nayak G, Charan S, et al. (2015) Phenotyping and 16S rDNA Analysis after Bioeld Treatment on

Citrobacter braakii: A Urinary Pathogen. J Clin Med Genom 3: 129. doi: 10.4172/jcmg.1000129

Volume 3 * Issue 1 * 1000129

ISSN: IJGM, an open access journal

as 4A) using genomic purication kit, according to the manufacturer

instructions. 16S rDNA gene (~ 1.5 kb) fragment was amplied with the

help of high-delity polymerase chain reaction (PCR) using universal

primers; forward primer (5'-AGAGTTTGATCCTGGCTCAG-3') and

reverse primer (3'-ACGGTCATACCTTGTTACGACTT-5'). Amplied

products were subjected to gel electrophoresis in 1.0% agarose gel,

stained with ethidium bromide and visualized under UV light in a gel

documentation unit (BioRad Laboratories, USA). e PCR amplied

fragment was puried from the agarose gel using a DNA gel extraction

kit. Sequencing of amplied product was done on commercial basis

from Bangalore Genei, India. e 16S rDNA sequences obtained were

aligned and compared with the sequences stored in GenBank database

available from National Center for Biotechnology Information (NCBI)

using the algorithm BLASTn program. Multiple sequence alignment/

phylogenetic tree were established using MEGA3.1 molecular soware

Antimicrobial susceptibility test

e results of C. braakii susceptibility pattern and MIC values of

tested antimicrobials aer bioeld treatment are presented in Table 1

and 2 respectively. e data were analyzed and compared with respect

to control (Gr. I). Antimicrobial susceptibility assay was carried out

using twenty-eight antimicrobials. Overall, the treated cells of C.

braakii showed 39.29% alteration in antimicrobial sensitivity pattern

as compared to control. e sensitivity pattern of tetracycline was

changed from resistance (R) to susceptible (S) and simultaneously

decreased MIC value by two folds (>8 to ≤ 4 µg/mL) in all the treated

groups as compared to control (Gr. I). is improvement with

respect to resistant pattern and MIC value could be due to bioeld

treatment. e eect was observed throughout the experiment. So,

it may assume that the eect of bioeld treatment was sustainable.

Moreover, the antibiogram pattern of certain antimicrobials viz.

aztreonam, cefotaxime, cefotetan, ceazidime, ceriaxone, cefuroxime,

piperacillin/tazobactam, piperacillin and ticarcillin/k-clavulanate were

changed from inducible β-lactamase (IB) to susceptible in lyophilized

treated (rst treatment) Gr. IIB, Study I on day 159 as well as in revived

treated (second treatment) Gr. IIB, Study II on day 5 as compared

to untreated sample (Gr. I). e microbe C. braakii has the ability to

produce chromosomal β-lactamases. us, the overproduction of

this enzymes lead to resistance in most of penem and cephems ring

containing antimicrobials [34].

However, aer bioeld treatment the above mentioned nine

antimicrobials were converted from IB to completely susceptible

in lyophilized treated (rst treatment) Gr. IIB, Study I on day 159 as

well as in revived treated (second treatment) Gr. IIB, Study II on day

5 as compared to control sample. However, the susceptibility pattern

of these nine antimicrobials did not show any alteration in rest of

the treated samples as compared to control. is alteration could be

due to exertion of bioeld energy to the treated samples at enzymatic

S. No.AntimicrobialGr. I (Control)Gr. IIA (Day 10)Gr. IIB (Study I; Day

Gr. IIB (Study II; Day 159)

3.Ampicillin/sulbactamI I I I I I

4.AmpicillinR R R R R R

8. CefotaximeIB IB S S IB IB

12. CeftriaxoneIBIBSSIBIB

15.Chloramphenicol S S S S S S

16. Ciprooxacin S S S S S S

18. GentamicinS S S S S S

20. Levooxacin S S S S S S

21.MeropenemS S S S S S

24.PiperacillinIB IB SSIB IB

25.TetracyclineR S S S S S

26. Ticarcillin/k-clavulanateIBIBSSIBIB

27.TobramycinS S S S S S

/sulphamethoxazole S S S S S S

R: Resistant; S: Susceptible; I: Intermediate; IB: Inducible β-lactamase; Gr.: Group

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii - PdfSR.com

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	




	
	
		Phenotyping Analysis of Citrobacter Braakii after Biofield Treatment
	

# data[].attributes.subject
	
	
	
	




	
	
		Phenotyping Analysis of Citrobacter Braakii after Biofield Treatment
	

# data[].attributes.body
| Read here the impact of the biofield treatment on phenotyping characteristic of Citrobacter braakii. The study present here was intended to alter the various biochemical properties of Citrobacter Braakii using the alternative energy treatment.

# data[].attributes.attachment_link.keywords[]
16s rdna analysis of citrobacter braakii

# data[].attributes.attachment_link.title
"Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number.

# data[].attributes.headline
	
	
	
	




	
	
		Citrobacter Braakii Antimicrobial Susceptibility Test
	

# data[].attributes.subject
	
	
	
	




	
	
		Citrobacter Braakii Antimicrobial Susceptibility Test
	

# data[].attributes.body
| The results of C. braakii antimicrobial susceptibility pattern and MIC values of tested antimicrobials after biofield treatment are presented in this study. Overall antimicrobial susceptibility pattern and MIC values showed positive alterations as a result of biofield energy.

# data[].attributes.headline
	
	
	
	




	
	
		16s rDNA Analysis of Citrobacter Braakii
	

# data[].attributes.subject
	
	
	
	




	
	
		16s rDNA Analysis of Citrobacter Braakii
	

# data[].attributes.body
| The present work was undertaken to evaluate the impact of unique energy treatment on C. Braakii in relation to antimicrobials susceptibility and biotyping based on various biochemical characters followed by 16S rDNA sequencing analysis.

# data[].attributes.attachment_link.title
Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

# data[].attributes.attachment_link.description
Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi's biofield treatment on C. braakii in lyophilized as well as revived state for

# data[].attributes.headline
	
	
	
	




	
	
		Antimicrobial Susceptibility Testing of Citrobacter Braakii
	

# data[].attributes.subject
	
	
	
	




	
	
		Antimicrobial Susceptibility Testing of Citrobacter Braakii
	

# data[].attributes.body
| The current study was attempted to investigate the effect of human energy treatment on C. Braakii. The results were obtained and analyzed using antimicrobial susceptibility testing and 16S rDNA analysis.

# data[].attributes.headline
	
	
	
	




	
	
		Phenotyping and 16S rDNA Analysis of Citrobacter Braakii
	

# data[].attributes.subject
	
	
	
	




	
	
		Phenotyping and 16S rDNA Analysis of Citrobacter Braakii
	

# data[].attributes.body
| Phenotyping and 16S rDNA Analysis of human energy treated Citrobacter Braakii shows significant alterations in antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Read here for more details.

# data[].attributes.headline
	
	
	
	




	
	
		Antimicrobial Susceptibility Testing of Burkholderia Cepacia
	

# data[].attributes.subject
	
	
	
	




	
	
		Antimicrobial Susceptibility Testing of Burkholderia Cepacia
	

# data[].attributes.body
| Burkholderia cepacia antimicrobial susceptibility testing was conducted using MicroScan Walk-Away®. The human energy treatment on MDR strain of B. cepacia showed a significant change in sensitivity pattern of different antimicrobials.

# data[].attributes.attachment_link.keywords[]
phenotypic characteristics of b. cepacia

# data[].attributes.attachment_link.title
"An Effect of Biofield Treatment on Multidrug-resistant Burkholderia cepacia: A Multihost Pathogen" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B.

# data[].attributes.headline
	
	
	
	




	
	
		Energy Healing Impact on Biotype Number of B. Cepacia
	

# data[].attributes.subject
	
	
	
	




	
	
		Energy Healing Impact on Biotype Number of B. Cepacia
	

# data[].attributes.body
| The present study was aimed to evaluate the impact of biofield treatment on antimicrobial susceptibility pattern, MIC values, biochemical reactions and biotype number of MDR strain of B. cepacia. Read here the complete study paper.

# data[].attributes.headline
	
	
	
	




	
	
		Burkholderia Cepacia Biochemical Characteristics
	

# data[].attributes.subject
	
	
	
	




	
	
		Burkholderia Cepacia Biochemical Characteristics
	

# data[].attributes.body
| The findings of the present study suggest that the unique energy treatment has a significant effect on the different biochemical characteristics of the multidrug-resistant strain of B. cepacia.

# data[].attributes.headline
	
	
	
	




	
	
		Burkholderia Cepacia Biochemical Reactions after Human Energy Treatment
	

# data[].attributes.subject
	
	
	
	




	
	
		Burkholderia Cepacia Biochemical Reactions after Human Energy Treatment
	

# data[].attributes.body
| Read here the impact of unique human energy treatment on biochemical reactions of Burkholderia cepacia. Overall results showed an alteration of 18.2% change in biochemical reaction after biofield treatment. Visit for more detailed information.

# data[].attributes.headline
	
	
	
	




	
	
		Burkholderia Cepacia Antimicrobial Susceptibility Testing
	

# data[].attributes.subject
	
	
	
	




	
	
		Burkholderia Cepacia Antimicrobial Susceptibility Testing
	

# data[].attributes.body
| Visit the link to know about the effects of unique human energy treatment on Burkholderia cepacia to the antimicrobial susceptibility. The result shows a significant change in sensitivity pattern of different antimicrobials.

# data[].attributes.headline
	
	
	
	




	
	
		Multidrug Resistant B. Cepacia - Impact of Unique Healing Energy
	

# data[].attributes.subject
	
	
	
	




	
	
		Multidrug Resistant B. Cepacia - Impact of Unique Healing Energy
	

# data[].attributes.body
| The impact of unique energy healing treatment on multidrug resistant B. cepacia was studied and evaluated the results as compared to the untreated samples.

# data[].attributes.headline
	
	
	
	




	
	
		Characterization of Physical and Structural Properties of Brass Powder
	

# data[].attributes.subject
	
	
	
	




	
	
		Characterization of Physical and Structural Properties of Brass Powder
	

# data[].attributes.body
| The unique human energy treatment of Brass Powder shows changes in its physical and structural properties. Visit the link for more details and information.

# data[].attributes.attachment_link.title
Characterization of Physical and Structural Properties of Brass Powder After Biofield Treatment | Mahendra Trivedi | Gopal Nayak | Shrikant Patil | Rama Mohan Tallapragada | Omprakash Latiyal | Academic Room

# data[].attributes.headline
	
	
	
	




	
	
		Human Energy Treatment of Brass Powder & X-Ray Diffraction Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		Human Energy Treatment of Brass Powder & X-Ray Diffraction Analysis
	

# data[].attributes.body
| The XRD analysis of human energy treated brass samples shows an increase in lattice parameter and unit cell volume up to 0.06% and 0.19%, respectively as compared to untreated samples. Read here for more information.

# data[].attributes.headline
	
	
	
	




	
	
		Study of Physical and Structural Properties of Brass Powder
	

# data[].attributes.subject
	
	
	
	




	
	
		Study of Physical and Structural Properties of Brass Powder
	

# data[].attributes.body
| Brass is one of the widely used alloys because of its properties. The present study was aimed to evaluate the impact of unique energy healing on the physical and structural properties of brass in order to further improve the properties.

# data[].attributes.headline
	
	
	
	




	
	
		Brass Powder Energy Healing Therapy & X-Ray Diffraction Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		Brass Powder Energy Healing Therapy & X-Ray Diffraction Analysis
	

# data[].attributes.body
| In this study, the impact of unique energy healing treatment was evaluated on structural and physical properties of the brass powder. X-Ray diffraction analysis of energy treated brass powder shows positive alterations in crystallite size.

# data[].attributes.attachment_link.keywords[]
x-ray diffraction analysis of brass powder

# data[].attributes.attachment_link.title
"Characterization of Physical and Structural Properties of Brass Powder After Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Brass, a copper-zinc (Cu-Zn) alloy has gained extensive attention in industries due to its high corrosion resistance, machinability and strength to weight ratio. The aim of present study was to evaluate the effect of biofield treatment on structural and physical properties of brass powder. The brass powder sample was divided into two parts: control and treated.

# data[].attributes.headline
	
	
	
	




	
	
		Characterization of Brass Powder Properties
	

# data[].attributes.subject
	
	
	
	




	
	
		Characterization of Brass Powder Properties
	

# data[].attributes.body
| The characterization of Brass powder after the unique energy healing treatment of brass samples shows some significant changes in the physical and Structural Properties of Brass. Follow the link for more information.

# data[].attributes.headline
	
	
	
	




	
	
		Brass Powder X-Ray Diffraction Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		Brass Powder X-Ray Diffraction Analysis
	

# data[].attributes.body
| In this experiment, X-Ray diffraction analysis of biofield treated brass powder shows a significant improvement in the crystallite size of brass sample. Visit to know more detailed information.

# data[].attributes.attachment_link.title
Characterization of Physical and Structural Properties of Brass Powder After Biofield Treatment

# data[].attributes.attachment_link.description
Brass, a copper-zinc (Cu-Zn) alloy has gained extensive attention in industries due to its high corrosion resistance, machinability and strength to weight ratio. The aim of present study was to evaluate the effect of biofield treatment on structural and physical properties of brass powder. The brass powder sample was divided into two parts: control and treated.

# data[].attributes.headline
	
	
	
	




	
	
		Changes in Physical and Structural Properties of Brass
	

# data[].attributes.subject
	
	
	
	




	
	
		Changes in Physical and Structural Properties of Brass
	

# data[].attributes.body
| Read here how unique energy healing has altered the physical and structural properties of the brass powder and improve its usability for automobile and transport equipment applications.

# data[].attributes.attachment_link.title
Characterization of Physical and Structural Properties of Brass Powder After Biofield Treatment

# data[].attributes.attachment_link.description
Brass, a copper-zinc (Cu-Zn) alloy has gained extensive attention in industries due to its high corrosion resistance, machinability and strength to weight ratio. The aim of present study was to evaluate the effect of biofield treatment on structural

# data[].attributes.headline
	
	
	
	




	
	
		Human Energy Impact on Structural Properties of Brass Powder
	

# data[].attributes.subject
	
	
	
	




	
	
		Human Energy Impact on Structural Properties of Brass Powder
	

# data[].attributes.body
| The study reports the influence of unique human energy treatment on the physical and structural properties of brass powders. Visit for more details.

# data[].attributes.headline
	
	
	
	




	
	
		X-Ray Diffraction Analysis of Brass Powder
	

# data[].attributes.subject
	
	
	
	




	
	
		X-Ray Diffraction Analysis of Brass Powder
	

# data[].attributes.body
| X-Ray Diffraction Analysis of Brass Powder was conducted in this study to evaluate the impact of biofield treatment on the crystallite size of Brass samples.

# data[].attributes.headline
	
	
	
	




	
	
		Evaluation of Physical Properties of Selenium
	

# data[].attributes.subject
	
	
	
	




	
	
		Evaluation of Physical Properties of Selenium
	

# data[].attributes.body
| The study evaluates the alteration in thermal & physical properties of Selenium after its treatment with the unique energy transmission method. Visit the link for more details.

# data[].attributes.attachment_link.readable_text
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Journal of Food and Nutrition Sciences

Published online November 9, 2015 (http://www.sciencepublishinggroup.com/j/jfns)

doi: 10.11648/j.jfns.20150306.14

ISSN: 2330-7285 (Print); ISSN: 2330-7293 (Online)

Evaluation of Biofield Energy Treatment on Physical and

Thermal Characteristics of Selenium Powder

, Rama Mohan Tallapragada

Trivedi Global Inc., Henderson, USA

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana.

Vol. 3, No. 6, 2015, pp. 223-228. doi: 10.11648/j.jfns.20150306.14

Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various

diseases, such as cancer and heart diseases. The ob jective of this study was to investigate the influence of biofield energy

treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts

treatment. Both control and treated selenium samples were characterized using x-ray diffraction (XRD) , differential scanning

calorimetry (DSC), thermogravimetric analysis - differential thermal analysis (TGA-DTA), and Fourier transform i nfrared

spectroscopy (FT-IR). The XRD data showed that biofield energy treatment has slightly altered the lattice parameter (0.07%),

unit cell volume (0.15%), density (-0.14%), atomic weight (0.15%), and nuclear charge per unit volume (-0.21%) in the treated

selenium powder as compared to the control. The crystallite size of the treated selenium powder was reduced considerably from

106.98 nm (control) to 47.55 nm. The thermal analysis study showed that the latent heat of fusion was 64.61 J/g in the control,

which changed to 68.98, 52.70, 49.71 and 72.47 J/g in the treated T1, T2, T3, and T4 samples respectively. However, the melting

temperature did not show any considerable change in the treated selenium samples as compared to the control. The FT-IR spectra

showed the absorption peak at 526 and 461 cm

corresponding to metal oxide bonding vibration in the control and treated

selenium powder respectively. Hence, overall data suggest that, the biofield energy treatment considerably altered the physical

and thermal properties of selenium powder. Therefore, biofield energy treatment could make selenium even more useful nutrient

Biofield Energy Treatment, Selenium Powder, X-ray Diffraction,

Thermogravimetric Analysis - Differential Thermal Analysis, Differential Scanning Calorimetry,

Fourier Transform Infrared

established, and i ts deficie ncy has caused serious diseases

such as cancer and heart disease [1]. Ar ound 25

selenoproteins contain seleniu m as important element, which

are commonly known a s selenocysteine [2 ]. In addition,

research on sel enium has gained significant atte ntion due to

its important role in antioxidant selenop roteins for protection

against oxidative stress initiated by excess reac tive nitrogen

species (RNS) and r eactive oxygen species (ROS) [1]. In

selenocysteins, selenium metal functions as redox centre in

various processes, which include s the reductio n of

nucleotides in DNA synthesis thro ugh selenoenzyme and

thioredoxin reductase; and p roduction o f active thyroid

hormone pre-cursor from sele nium-dependent iodothyro nine

deiodinases [3]. Furthermo re, the bioavailability of se lenium

plays a crucial role in its anticancer and antioxi dant activities.

toxicity as compared to pa rticles with large crystallite size

[4-6]. After considering th e vast impo rtance of selenium as

economically sa fe approach that could be benefici al to

Thermal Characteristics of Selenium Powder

modify the phys ical and the rmal properties of sele nium

It is well established that all atoms ar e in moti on, which

contain signifi cant amount of energ y. This energ y exists in

the for m of translatio nal, ro tation o r vibra tional, which is

evident from vibration al spectroscopy. Similarly, cells o f

human body cons ist of various vibratory par ticles including

electron, proton, ions etc. Further, d ue to vibrations of these

particles, electromagne tic radiation emitted a nd that form an

electromagnetic field around the bod y, known as biofield [7].

Thus, a human has the ability to harness the energy from the

environment/universe and can tra nsmit it to any object

(living or non-livin g) around the Globe. The object(s)

always receive the energy and responded it into useful way

that is called biofield energy. This energy healing proc ess is

considered this biofie ld treatment (therapy) in subcategory

of energy therapies [ 8]. Mr. Trivedi's unique biofield energy

is also known as The Trivedi Effect

energy trea tment is known to alter the physic al, struc tural

and atomic leve l in various metals [9 -11] and ceramics

[12,13] in mat erial science. In ad dition to tha t, impa ct of

biofield treatment has been sig nificantly studied in the field

of microbiology research [14], biotechnology resea rch [15],

and agriculture research [16] . Recently, it was reported that

biofield trea tment had enhanced the crystal lite size by two

fold in zinc powder [17]. I n addition, biof ield treatment ha s

significantly alte red the c rystallite size , unit cell parameter,

surface area of magnesium, which is an important

micronutrient for human body [18]. Based on the outstanding

ceramics, an attempt was made to evaluate the effect of

biofield treatment on physica l and thermal pr operties of

Thermogravimetric analysis - differential thermal analys is

(TGA-DTA), and Fourier transform infrared spectr oscopy

The selenium powder was purchased fro m Alpha Aesar,

Hyderabad, India. T he sample was equally divided into two

parts, considered as control and treatment. Control parts

remained as untreated, whereas treatment group was in sealed

pack and handed over to Mr. Trivedi for biofield treatment

under laboratory condition. Mr. Trivedi provide d the biofield

treatment through his energy transmission process to the

treated group without to uching. The control and treated

samples were characterized using XRD, DSC, TGA-DTA, and

2.1. X-ray Diffraction Study

XRD analysis of control and treated selenium powder was

carried out on Phillips, Holland PW 1710 X-ray diffractometer

system, which had a copper anode with nickel filter. The

1.54056Å. The data obtained from the XRD system were in

the form of a chart of 2θ vs. intensity a nd a detailed table

containing peak intensity counts, d value (Å), peak width (θ

relative inte nsity (%) etc . Ad ditionally, PowderX software

was used to calculate the lattice parameter a nd unit cell

volume of selenium powder samples.Total nuclear charge was

calculated as the number of protons multiplied by charge on a

C). Nuclear charge per unit volume was

Nuclearchargeperunitvolume

=Totalnuclearchargeinanatom

Further, the crystallite size (G) was calculated by using

Here, λ is the wavelength of radiation used, b is full width

half maximum (FWHM) and k is the equipment constant

parameter was calculated using following equation:

%changeinlatticeparameter

are the lattice parameter of

percent change in all other parameters such as unit cell volume,

density, atomic weight, nuclear charge per unit volume and

crystallite size were calculated.

Thermal analysis of control and treated selenium powder

were performed using TGA-DTA and DSC. For DSC analysis,

a Pyris-6 Perkin Elmer DSC at a heating rate of 10ºC/min under

air atmosphere was used. Predetermined amount of sample was

kept in an aluminum pan and closed with a lid. A blank

aluminum pan was used as a reference. From DSC melting

point and latent heat of fusion were recorded. For TGA-DTA

analysis, Mettler Toledo simultaneous TGA and Differential

thermal analyser (DTA) was used. The samples were heated

from room temperature to 400ºC with a heating rate of 5ºC/min

under air atmosphere. From TGA curve, percent weight loss

and treated selenium were recorded from DTA curve. The

percent change in melting point was computed using following

%changeinmeltngpoint =  T

are the melting point of control

and treated samples, respectively. Similarly, t he percent

change in the latent heat of fusion was computed.

Journal of Food and Nutrition Sciences 2015; 3(6): 223-228 225

2.3. Fourier Transform Infrared Spectroscopy (FT-IR)

FT-IR spectroscopic analysis was carried out to evaluate the

impact of biofield treatment at atomic and molecular level like

bond strength, stability, and rigidity of structure etc. FT-IR

performed on Shimadzu, Fourier transform infrared (FT-IR)

spectrometer with frequency range of 300-4000 cm

3. Results and Discussion

3.1. X-ray Diffraction (XRD)

XRD pattern of control and treated selenium samples are

shown in Figure 1a and 1b respectively. In XRD pattern of

control sample, peaks were observed at 2θ equal to 23.56°,

29.66°, 41.43°, 43.65°, 45.33°, 51.72°, and 56.20°. However,

XRD pattern of treated selenium sample showed peaks at 2θ

equal to 23.43°, 23.51°, 29.69°, 41.30°, 43.57°,45.43°, 51.73°

and 55.64°. The XRD pattern indicated the hexagonal crystal

structure (JCPDS file no. 86-2246) in control and treated

control and treated sample suggested the cr ystalline nature of

selenium powder. Crystal structure parameters were computed

using PowderX software such as lattice parameter, unit cell

volume, density, atomic weight, nuclear charge per unit

volume. The results a re presented in Table 1. Result showed

that lattice parameter and volume of unit cell were slightly

increased by 0.07 and 0.15% in treated selenium as compared

to control. The increase of unit cell volume may induce

internal strain in treated selenium powder. In addition, the

atomic weight of treated selenium was slightly increased by

0.15% as compared to control.

Fig 1. X-ray diffraction pattern of selenium powder (a) control (b) treated.

Table 1. X-ray diffraction analysis of control and treated selenium powder.

However, density and nucl ear charge per unit vol ume was

decreased b y 0.14 and 0.21%, respectivel y as c ompared to

formula was found as 106.98 nm in cont rol and it was

reduced to 47.55 nm in treated sele nium powder. It suggests

that crystallite size of treated selenium sample was

significantly reduced by 55.5 5% as compared to control.

structure [19]. Thus, it is assu med t hat bi ofield treatment

might ind uce ene rgy millin g in selenium powder and that

might be responsible for decrease in crystalli te size of treated

selenium powder. Rec ently, our group reported that b iofield

treatment had r educed the crystal lite size in aluminium [20]

and magnesium powder [17].

Moreover, selenium is a prim ary nutrient for human bo dy,

demonstrated that the rate of dissolu tion can be a ltered by

choosing a suitable polymorph of a comp ound i.e. the solid s

with low crystallini ty or high amorp hous phase exhibi ts

higher solubility [21]. Torrado et al. rep orted that solids with

small crysta llite size exhibits faster disso lution rate as

compared to soli ds with higher crystallite size [22]. Thus, it

is hypothesiz ed that biofield treated selenium p owder may

dissolution r ate may lead to inc rease bioava ilability of

selenium in human gast ric fluids.

Thermal analysis of s elenium powder was carried ou t

using TGA-DTA and DSC. Thermal a nalysis results of

control and treated s elenium powder samples using DSC are

# data[].attributes.attachment_link.authors[].name
Mahendra Kumar Trivedi, Rama Mohan Tallapragada, Alice Branton, Dahryn Trivedi, Gopal Nayak, Omprakash Latiyal, Snehasis Jana

# data[].attributes.attachment_link.title
Evaluation of Biofield Energy Treatment on Physical and Thermal Characteristics of Selenium Powder - PdfSR.com

# data[].attributes.attachment_link.description
Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder.

# data[].attributes.headline
	
	
	
	




	
	
		Selenium Powder Analysis after the Energy Treatment
	

# data[].attributes.subject
	
	
	
	




	
	
		Selenium Powder Analysis after the Energy Treatment
	

# data[].attributes.body
| In this study, the selenium powder analysis was conducted to observe the changes in its physical & thermal characteristics under the influence of unique energy treatment. Visit the link for more details.

# data[].attributes.headline
	
	
	
	




	
	
		Selenium XRD Analysis: Assessment of Physical Characteristics
	

# data[].attributes.subject
	
	
	
	




	
	
		Selenium XRD Analysis: Assessment of Physical Characteristics
	

# data[].attributes.body
| Selenium XRD analysis results show positive changes in lattice parameter, unit cell volume, density, atomic weight and nuclear charge per unit volume in treated selenium as compared to control after the influence of energy treatment.

# data[].attributes.attachment_link.title
Evaluation of Biofield Energy Treatment on Physical and Thermal Characteristics of Selenium Powder

# data[].attributes.attachment_link.description
Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment

# data[].attributes.headline
	
	
	
	




	
	
		Physical & Thermal Characteristics of Selenium
	

# data[].attributes.subject
	
	
	
	




	
	
		Physical & Thermal Characteristics of Selenium
	

# data[].attributes.body
| The main objective of this study was to investigate the influence of human energy treatment on the physical and thermal characteristics of the selenium powder. Visit for more information.

# data[].attributes.headline
	
	
	
	




	
	
		FT-IR Analysis of Biofield Treated Tetracycline
	

# data[].attributes.subject
	
	
	
	




	
	
		FT-IR Analysis of Biofield Treated Tetracycline
	

# data[].attributes.body
| The Tetracycline FT-IR analysis shows significant alteration in various spectral properties of this antimicrobial, under the influence of Biofield Energy. The same phenomenon was also applied on Chloramphenicol to study the biofield changes.

# data[].attributes.attachment_link.title
"Spectroscopic Characterization of Chloramphenicol and Tetracycline: An Impact of Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy.

# data[].attributes.headline
	
	
	
	




	
	
		Tetracycline UV Spectrum Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		Tetracycline UV Spectrum Analysis
	

# data[].attributes.body
| The UV-Vis spectroscopic analysis of Tetracycline & Chloramphenicol was conducted to evaluate the effect of unique energy treatment on structural properties of these antimicrobials.

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Chloramphenicol and Tetracycline: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy.

# data[].attributes.headline
	
	
	
	




	
	
		Chloramphenicol FT-IR Analysis -Study of Biofield Impact
	

# data[].attributes.subject
	
	
	
	




	
	
		Chloramphenicol FT-IR Analysis -Study of Biofield Impact
	

# data[].attributes.body
| The chloramphenicol FT-IR analysis of antibiotics shows decrease in wavenumber of NO2 from 1521 cm-1 to 1512 cm-1 and increase in wavenumber of C=O from 1681 cm-1 to 1694 cm-1. Read here the detailed analysis.

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Chloramphenicol and Tetracycline: An Impact of Biofield Treatment

# data[].attributes.attachment_link.description
Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed

# data[].attributes.headline
	
	
	
	




	
	
		Healing Impact on Spectroscopic Characterization of Tetracycline
	

# data[].attributes.subject
	
	
	
	




	
	
		Healing Impact on Spectroscopic Characterization of Tetracycline
	

# data[].attributes.body
| The analysis shows that Mahendra Trivedi's energy healing phenomena resulting change in spectroscopic characterization of Tetracycline. Visit the link for more details.

# data[].attributes.headline
	
	
	
	




	
	
		FT-IR Analysis of Chloramphenicol & Tetracycline
	

# data[].attributes.subject
	
	
	
	




	
	
		FT-IR Analysis of Chloramphenicol & Tetracycline
	

# data[].attributes.body
| The Tetracycline & Chloramphenicol were subjected to the FT-IR analysis to evaluate the impact of biofield treatment at the atomic level like bond strength, stability, and rigidity of the chemical structure.

# data[].attributes.headline
	
	
	
	




	
	
		Assessment in Spectroscopic Characterization of Chloramphenicol
	

# data[].attributes.subject
	
	
	
	




	
	
		Assessment in Spectroscopic Characterization of Chloramphenicol
	

# data[].attributes.body
| The main concept of this study is to overcome the microbial resistance of two antibiotics i.e. chloramphenicol and tetracycline, with the help of biofield energy treatment.

# data[].attributes.attachment_link.readable_text
Please enable JavaScript to view the comments powered by Disqus.

Trivedi et al., Pharm Anal Acta 2015, 6:7

http://dx.doi.org/10.4172/2153-2435.1000395

Volume 6 * Issue 7 * 1000395

ISSN: 2153-2435 PAA, an open access journal

Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety

of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline.

The present study was aimed to evaluate the impact of bioeld treatment for spectroscopic characterization of

chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy.

Methods: The study was performed in two groups (control and treatment) of each antibiotic. The control groups

remained as untreated, and bioeld treatment was given to treatment groups.

Results: FT-IR spectrum of treated chloramphenicol exhibited the decrease in wavenumber of NO2 from 1521

due to increase of conjugation effect in NO2 group, and increased force constant of C=O bond. As a result, stability of

both NO2 and C=O groups might be increased in treated sample as compared to control. FT-IR spectrum of treated

tetracycline showed the downstream shifting of aromatic C-H stretching from 3085-3024 cm-1 to 3064-3003 cm-1 and

It may be due to enhanced conjugation effect in tetracycline, and increased force constant of C-N (CH3) bond of

tetracycline as compared to control. The results indicated the enhanced stability of treated tetracycline as compared

to control. UV-Vis spectra of bioeld treated chloramphenicol and tetracycline showed the similar lambda max (λmax )

to their respective control. It revealed that the chromophore groups of both antibiotics remained same as control

after the bioeld treatment.

Conclusion: Based on FT-IR spectroscopic data, it is speculated that due to increase in bond strength and

conjugation effect after bioeld treatment, the chemical stability of both the drugs might be increased as compared

Spectroscopic Characterization of Chloramphenicol and Tetracycline: An

Impact of Biofield Treatment

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Keywords: Chloramphenicol; Tetracycline; Bioeld treatment;

Fourier transform infrared spectroscopy; Ultraviolet spectroscopy

Chloramphenicol and tetracycline are structurally dissimilar broad-

spectrum antibiotics that commonly act by inhibiting the protein synthesis

in microbes. ese are extensively used in several microbial infections

including Gram-negative, Gram-positive bacteria, chlamydiae, and

rickettsiae [1-3]. Chloramphenicol reversibly binds to 50S ribosomal

subunit of microbes and prevents the binding of aminoacyl tRNA to 50S

ribosomal subunit. us, it inhibits protein synthesis in bacteria, which

is essential for bacterial growth [3,4]. Despite of its broad spectrum

antimicrobial activities, it also has some adverse eects such as gray baby

syndrome, aplastic anemia, and bone marrow depression [3,5]. Nowadays,

chloramphenicol is not much eective due to development of resistance

in the variety of microbes. Microbial resistances against chloramphenicol

occur by several mechanisms like enzymatic (acetyltransferases and

phosphotransferases) inactivation; decreasing the membrane permeability,

mutation/modication in target site, and presence of eux pumps [6].

Tetracycline exerts both bacteriostatic and bactericidal mode of action

against the majority of aerobic, anaerobic, Gram-negative, and Gram-

positive bacteria. It binds with 30S ribosomal subunit of microbes and

block the binding of activated aminoacyl-tRNA to the A site of ribosome.

us, it blocks the insertion of new amino acids to the nascent peptide

chain [2,7,8]. Microbes acquire resistance to tetracycline by evolving

the eux pump and/or by ribosomal protection protein [9,10].

Antimicrobial resistance is now conceiving as global threat; as

a result need of new antimicrobial drugs is constantly increasing.

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,

Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006; Email:

Received June 10, 2015; Accepted July 21, 2015; Published July 24, 2015

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S, et al. (2015)

Spectroscopic Characterization of Chloramphenicol and Tetracycline: an Impact of

Bioeld. Pharm Anal Acta 6: 395. doi:10.4172/21532435.1000395

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

erefore, global scientic community has attempted to discover a

concept to overcome the microbial resistance i.e. reintroduction of

previously used antibiotics active against multi drug resistant (MDR)

bacteria. erefore, several antimicrobial agents like chloramphenicol,

tetracycline, etc are reemerging aer some modications as valuable

alternatives for the treatment of dicult-to-treat microbial infections

Stability of drug is of great importance for its ecacy. In addition,

drug degradation may lead to byproducts formation. Abachi et al.

showed that stability of chloramphenicol was independent of pH

between 4 to 6.2, and it was susceptible to hydrolysis in aqueous

media [13]. Lv et al. suggested that chloramphenicol was not stable in

suspension form [14]. Liang et al evaluated the stability of tetracycline

in methanol solution using UV-Visible spectroscopy, HPLC, and TLC

methods. e report showed that tetracycline decomposed quickly

Volume 6 * Issue 7 * 1000395

ISSN: 2153-2435 PAA, an open access journal

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana s, et al. (2015) Spectroscopic Characterization of Chloramphenicol and Tetracycline: an

Impact of Bioeld. Pharm Anal Acta 6: 395. doi:10.4172/21532435.1000395

with the inuence of light and atmospheric oxygen, and formed several

degradation products [15,16]. erefore, an alternative approach is needed

that can increase the shelf life of poorly stable drug.

Recently an alternative approach, bioeld treatment is recognized to

change the various physical and structural properties at the atomic level

of various living and non-living things. It is well established that electrical

current coexist along with the magnetic eld inside the human body in the

form of vibratory energy particles like ions, protons, and electrons [16,17].

Willem Einthoven discovered an electrocardiography in 1924 to measure

the human bioeld. Later on, Harold Saton Burr gave the hypothesis that

every dynamic process in the human body had an electrical signicance.

Recently, it conrmed that all the electrical process happening in human

body generates magnetic eld [18]. It can be observed using some

medical technologies such as electrocardiography, electromyography,

and electroencephalogram. e electromagnetic eld of the human body

is known as bioeld and energy linked with this eld is called bioeld

energy [19-21]. us, a human has the ability to harness the energy from

environment or universe and can transmit into any living or nonliving

object around this globe. e object(s) always receive the energy and

responding into useful way; this process is known as bioeld treatment

(e Trivedi Eect®). Mr. Trivedi's bioeld treatment has altered the

physicochemical and structural properties of metals and ceramics [22-24].

e growth and anatomical characteristics of medicinal plant also changed

aer bioeld treatment [25]. e bioeld treatment enhanced the yield and

quality of agriculture product [26]. Moreover, the changes in antimicrobial

susceptibility and biotype number of pathogenic microbe have been

reported aer bioeld treatment [27].

Conceiving the concept of antimicrobial reuses, the present study was

aimed to evaluate the impact of bioeld treatment on spectral properties of

two antibiotics i.e. chloramphenicol and tetracycline.

e chloramphenicol and tetracycline (Figure 1) samples were

procured from Sigma-Aldrich, MA, USA; and each antibiotic was

divided into two parts: control and treatment. e control samples

remained as untreated, and treatment samples were handed over in

sealed pack to Mr. Trivedi for bioeld treatment under laboratory

condition. Mr. Trivedi provided this treatment through his energy

transmission process to the treatment groups without touching the

objects. Aer that, the control and treated samples of each antibiotic

were analyzed using Fourier transform infrared (FT-IR) spectroscopy

and Ultraviolet-Visible (UV-Vis) spectroscopy.

FT-IR spectroscopic characterization

FT-IR spectra were recorded on Shimadzu's Fourier transform

infrared spectrometer (Japan) with frequency range of 4000-500 cm-1 .

e FT-IR spectral analysis of chloramphenicol and tetracycline were

carried out to evaluate the impact of bioeld treatment at atomic level

like bond strength, stability, and rigidity of chemical structure [28].

UV-Vis spectroscopic analysis

UV-Vis spectra of chloramphenicol and tetracycline were acquired

on a Shimadzu UV-2400 PC series spectrophotometer with 1 cm quartz

cell and a slit width of 2.0 nm. e analysis was carried out at wavelength

range of 200-400 nm. UV-Vis spectroscopic analysis was performed to

evaluate the eect of bioeld treatment on structural property of tested

antibiotics (chloramphenicol and tetracycline) [28].

FT-IR spectroscopic analysis

FT-IR spectra of control and treated chloramphenicol are shown

in Figure 2. FT-IR spectrum of control sample showed absorption

peaks at 3352-3246 cm-1 that were assigned to O-H and N-H stretching,

respectively. IR peaks at 3081 cm-1 were assigned to aromatic C-H

stretching. Vibrational peaks at 1681 and 1559 cm-1 were assigned to

C=O and C=C stretching, respectively. Further, the NO2 and C-Cl

stretching were observed at 1521 cm-1 and 662 cm-1, respectively; and

N-H bending was appeared at 1518 cm-1. e FT-IR data of control

chloramphenicol was well supported by the literature data [29].

e FT-IR spectrum of treated sample of chloramphenicol showed

IR absorption peaks for O-H and N-H stretching at 3243 cm-1 and

aromatic C-H stretching was appeared at 3079 cm-1. e vibrational

Figure 1: Chemical structure of (a) chloramphenicol and (b) tetracycline.

Figure 2: FT-IR spectra of chloramphenicol (control and treated).

Volume 6 * Issue 7 * 1000395

ISSN: 2153-2435 PAA, an open access journal

Citation: Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana s, et al. (2015) Spectroscopic Characterization of Chloramphenicol and Tetracycline: an

Impact of Bioeld. Pharm Anal Acta 6: 395. doi:10.4172/21532435.1000395

peak of carbonyl group (C=O) and C=C stretching were attribute

to IR peaks at 1694 and 1560 cm-1, respectively. Additionally, NO2

stretching and N-H bending peaks were appeared at 1512 and 1510 cm-

1, respectively; and C-Cl stretching was appeared at 667 cm-1.

Altogether, the result suggested that C-H and C=C stretching

peaks of aromatic ring were observed in similar frequency region in

both control and treated sample of chloramphenicol. It indicated that

stability and rigidity of phenyl ring was retained in treated sample as

like to control. e vibrational peaks for C=O stretching (acylamino

group) was observed towards higher frequency region i.e. from 1681

to 1694 cm-1 that may be due to an increase in force constant of C=O

group in treated chloramphenicol. Further, FT-IR spectra of treated

chloramphenicol showed decrease in wavenumbers of NO2 i.e. from

1521 to 1512 cm-1. It might be occurred due to increase of conjugation

eect between NO2 group and phenyl ring [30]. Force constant is directly

proportional to bond strength [28]. As the force constant of C=O was

increased due to bioeld treatment, the stability of acylamino moiety

in treated chloramphenicol should also be increased. Additionally, the

conjugation eect between NO2 group and phenyl ring may lead to

increase in chemical stability of treated chloramphenicol as compared

e FT-IR spectra of control and treated samples of tetracycline

are shown in Figure 3. FT-IR spectrum of control sample showed the

absorption peaks for N-H and O-H stretching at 3341-3329 cm-1 and

aromatic C-H stretching at 3085-3024 cm-1 . e vibrational peaks at

2995-2863 cm-1 and 1648-1582 cm-1 were assigned to CH3 stretching

and C=C stretching, respectively. Aromatic C-H bending was appeared

at 1458 cm-1 and CH3 bending was appeared at 1357 cm-1. e aromatic

in plane and out plane deformation peaks were appeared at 1247-1000

cm-1 and 567-501 cm-1 , respectively.Vibrational peak at 965 cm-1 was

assigned to C-N stretching. e FT-IR data of control sample was well

supported by reported data [31].

e FT-IR spectra of treated tetracycline showed the vibrational

peaks at 3342-3325 cm-1 that were attributed to N-H and O-H stretching.

Vibrational peaks at 3064-3003 cm-1 and 2955-2835 cm-1 were assigned

to C-H and CH3 (methyl) stretching, respectively. C=C stretching peaks

were appeared at 1622-1569 cm-1. Bending vibrational peaks for C-H

and CH3 groups were appeared at 1454 and 1357 cm-1, respectively.

Vibrational peaks at 1247-1000 cm-1, and 995 cm-1 were assigned to C-H

in plane deformation and C-N stretching, respectively. e out plane

ring deformation peaks were appeared 567-501 cm-1.

e FT-IR data of control and treated tetracycline showed that N-H

and O-H stretching peaks are observed in the similar frequency region

in both samples. is suggests no changes in the amide and hydroxyl

group of treated tetracycline as compared to control. IR absorption

peaks for C-H (aromatic)stretching were shied to lower frequency i.e.

from 3085-3024 cm-1 to 3064-3003 cm-1 and likely the CH3 stretching

cm-1 . IR absorption peak for C=C stretching was also appeared at

lower frequency region i.e. form 1648-1582 cm-1 to 1622-1569 cm-1 in

treated sample as compared to control. It is well known that resonance

or conjugation of C=C double bond or carbonyl group provides the

more single bond character to C=C bond that lowers the force constant

of respective bond [28,30]. Further, resonance and/or conjugation

eect also provide the stability to chemical compound. erefore, it is

speculated that bioeld treated tetracycline might be more chemically

stable due to enhanced conjugation eect in C and D ring, with respect

to control. is could be correlated to enhancement in stability of treated

tetracycline with respect of control. e C-N stretching peak in treated

Figure 3: FT-IR spectra of tetracycline (control and treated).

tetracycline was observed at higher wavenumber than control (i.e. from

965 to 995 cm-1). It could be due to increased bond strength of C-N

(CH3 ) group that might be increased stability of treated tetracycline as

UV spectra of control and treated samples of chloramphenicol

are shown in Figure 4. It showed no signicant change in the lambda

max (λmax) of treated sample (273.8 nm) as compared to control (272.2

nm), which indicated no changes in chromophore group of treated

chloramphenicol with respect to control. UV spectra of control and

treated sample of tetracycline are shown in Figure 5. Both spectra

showed three absorption peaks at 362.60, 268.80, and 220.60 nm in

control sample and 362.60, 268.80, and 221 nm in treated sample, which

indicated similar pattern of UV absorbance in both the samples. is

suggested no changes in chromophore group of treated tetracycline

with respect to control. Overall, the UV spectra of both the antibiotics

showed no signicant changes in λmax as compared to control. Based

on this, it could be concluded functional groups or their position did

not altered in treated sample aer bioeld treatment. To the best of

our knowledge, this is the rst report showing an impact of bioeld

treatment on spectral properties (force constant, dipole moment, and

bond strength) of chloramphenicol and tetracycline as compared to

control.

Altogether, the FT-IR data showed an alteration in the wavenumber

of some functional groups like C=O and NO2 in chloramphenicol and

C=C and C-N (CH3) in tetracycline with respect to control groups. is

could be observed due to some alteration at the atomic level of both

# data[].attributes.attachment_link.title
Spectroscopic Characterization of Chloramphenicol and Tetracycline: An Impact of Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy.

# data[].attributes.headline
	
	
	
	




	
	
		Characteristics of Klebsiella Pneumoniae: Impact of Biofield
	

# data[].attributes.subject
	
	
	
	




	
	
		Characteristics of Klebsiella Pneumoniae: Impact of Biofield
	

# data[].attributes.body
| The study shows that due to the influence of energy treatment there was a significant impact on antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug-resistant isolates of K. Pneumoniae.

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Mayank Gangwar | Snehasis Jana | Academic Room

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

# data[].attributes.headline
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Analysis
	

# data[].attributes.subject
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Analysis
	

# data[].attributes.body
| Klebsiella Pneumoniae Biochemical test results of both control and healing energy treated groups are summarized in the present research paper. Visit to know more about the research and results.

# data[].attributes.attachment_link.title
Peer Evaluation : Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

# data[].attributes.attachment_link.description
Abstract : Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae)and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat inpatients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatmenton four MDR clinical lab isolates (LS) of K.

# data[].attributes.headline
	
	
	
	




	
	
		Klebsiella Pneumoniae Characteristics | Effects of External Energy
	

# data[].attributes.subject
	
	
	
	




	
	
		Klebsiella Pneumoniae Characteristics | Effects of External Energy
	

# data[].attributes.body
| The result of the present work showed a significant impact of external energy treatment on antimicrobial susceptibility pattern, MIC values, biochemical reactions, and biotype number in all the four clinical MDR lab isolates of K. pneumoniae.

# data[].attributes.attachment_link.title
"Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

# data[].attributes.headline
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Characteristics Assessment
	

# data[].attributes.subject
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Characteristics Assessment
	

# data[].attributes.body
| The Klebsiella Pneumoniae biochemical characteristics were observed to assess the human energy effects on MDR of Klebsiella Pneumoniae and significant positive changes were noticed.

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		Klebsiella Pneumoniae Antibiogram Typing
	

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		Klebsiella Pneumoniae Antibiogram Typing
	

# data[].attributes.body
| A significant change in antibiogram typing of Klebsiella Pneumoniae was reported in all four clinical isolates of MDR K. pneumoniae after biofield treatment as compared to control group.

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care

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		A Research on Characteristics of Klebsiella Pneumoniae
	

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		A Research on Characteristics of Klebsiella Pneumoniae
	

# data[].attributes.body
| The aim of the present study was to evaluate the impact of unique human energy transmission on various biochemical characteristics of K. Pneumoniae. Read here for more details.

# data[].attributes.headline
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Tests
	

# data[].attributes.subject
	
	
	
	




	
	
		Klebsiella Pneumoniae Biochemical Tests
	

# data[].attributes.body
| Based on the biochemical test results of Klebsiella Pneumoniae, significant alteration in biotype numbers were observed in all the human energy treated lab isolates as compared to control.

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		Biofield Impact on Klebsiella Pneumoniae Characteristics
	

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		Biofield Impact on Klebsiella Pneumoniae Characteristics
	

# data[].attributes.body
| This study evaluates the impact of biofield treatment on biochemical characteristics of Klebsiella Pneumoniae characteristics such as biochemical reactions, biotype numbers and etc.

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Please enable JavaScript to view the comments powered by Disqus.

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

Research ArticleOpen Access

Trivedi et al.,J Trop Dis 2015, 3:4

http://dx.doi.org/10.4173/2329-891X.1000173

Research Article Open Access

Journal of Tropical Diseases

Mahendra Kumar Trivedi1 , Alice Branton1 , Dahryn Trivedi1, Harish Shettigar1, Mayank Gangwar2 and Snehasis Jana2*

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research Laboratory

Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-

Citation: Trivedi MK, Branton A, Trivedi D, Shettigar H, Gangwar M, et al. (2015)

Antibiogram Typing and Biochemical Characterization of Klebsiella Pneumonia

after Bioeld Treatment. J Trop Dis 3: 173. doi:10.4173/2329891X.1000173

Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed

under the terms of the Creative Commons Attribution License, which permits

unrestricted use, distribution, and reproduction in any medium, provided the

original author and source are credited.

Keywords: Klebsiella pneumoniae; Bioeld Treatment; Multidrug-

Resistant; Antimicrobial susceptibility; Biochemical Reaction;

shaped, facultative anaerobic, and nonmotile bacterium, belongs to

family Enterobacteriaceae . It is a common human pathogen associated

with nosocomial and community infections [1]. K. pneumoniae isolates

causes several infections such as pneumonia, septicemia, wound

infections, and urinary tract infections, which ultimately lead to

morbidity and mortality especially in immunocompromised patients,

and patients of intensive care units, pediatrics and surgical wards [2].

K. pneumoniae acquire resistance against existing antimicrobials by

multiple mechanism results in increased multidrug-resistant (MDR)

of K. pneumoniae thatleads to serious problem in hospital settings

and health concern. Emergence of resistance occurs not only in

MDR isolates but also exist in pan-drug resistant (PDR) isolates of K.

pneumoniae. PDR refers to the resistant strains those are specically

resistant to 7 antimicrobial agents such as cefepime, imipenem,

meropenem, ceazidime, ciprooxacin, piperacillin-tazobactam,

and levooxacin [3]. Apart from this, the extended-spectrum

β-lactamase (ESBL) producing Klebsiella from a patient has been

identied which causes serious threat worldwide [4,5]. Continuous

use of antibiotics leads to resistance in microorganisms via. dierent

pathways mediated by plasmids, transposons, and gene cassettes in

integrons [6,7]. Carbapenem is usually preferred for the infection

caused by MDRisolates of K. pneumoniae but recently carbapenem-

resistant K. pneumoniae was also reported [8]. Due to dramatically

increase in drug resistant in K. pneumoniae, very few treatment

options are available. Alternative approaches are available but altering

the sensitivity pattern of antimicrobials using bioeld is not available

against MDR microorganism, apart from existing allopathic system of

medicine. Bioeld treatment may be an alternative approach to alter

the susceptibility pattern of K. pneumoniae. Complementary and

alternative medicine (CAM) therapies are commonly practiced in

healthcare sector and about 36% of Americans regularly uses some form

of CAM [9]. CAM include numerous energy therapies, bioeld therapy,

is one of the energy medicine widely used worldwide to improve the

human health. e energy exists in various forms that can be produced

from dierent sources such as potential, electrical, kinetic, magnetic,

and nuclear energy. However, electromagnetic eld denes as when

electrical signals uctuate will generate magnetic eld with respect

to time. e cumulative eect of bio-magnetic and electric eld that

surrounds the human body is dened as bioeld. e bioeld energy can

be monitored by using electromyography (EMG), electrocardiography

(ECG) and electroencephalogram (EEG) [10]. According to Lucchetti

et al. bioeld energy has shown signicant eect on growth of bacterial

cultures [11]. Mr. Trivedi has the ability to harness the energy from

environment or universe and can transmit into any living or nonliving

object(s) around the Universe. e objects always receive the energy

and responding into useful way via bioeld energy and the process is

known as bioeld treatment. Mr. Trivedi's unique bioeld treatment is

extensively studied in dierent elds such as in material science [12,13],

agricultural science [14-16], and in biotechnology [17]. Further,

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae)

patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's bioeld treatment

on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into

two groups i.e. control and bioeld treated. Control and treated groups were analyzed for antimicrobial susceptibility

pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away®

system. The analysis was done on day 10 after bioeld treatment as compared with control group. Antimicrobial

sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of

treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was

reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in

biochemical reactions as compared to control. A signicant change in biotype numbers were reported in all four clinical

number after bioeld treatment, new organism was identied as Enterobacter aerogenes in LS 2 and LS 14. These

results suggest that bioeld treatment has a signicant effect on altering the antimicrobial sensitivity, MIC values,

biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

the bioeld treatment has considerably altered the susceptibility of

antimicrobials and biotype of microbes [18-20]. By considering the

above mentioned facts and literature reports on bioeld treatment,

the present work was undertaken to evaluate the impact of bioeld

treatment on antimicrobials susceptibility, biochemical reactions

pattern, and biotype of MDR isolates of K. pneumoniae.

Experimental design and bacterial isolates

MDR clinical lab isolates (i.e. LS 2, LS 6, LS 7 and LS 14) of K.

pneumoniae were obtained from stored stock cultures in Microbiology

Lab, Hinduja Hospital, Mumbai. Each MDR strains was divided

into two groups i.e. control and treatment. e acceptability of the

identication media and antimicrobial agents were checked prior to the

study on microorganisms. e antimicrobial susceptibility, biochemical

reactions, and biotype number were evaluated on MicroScan Walk-

Away® (Dade Behring Inc., West Sacramento, CA) using Negative

Breakpoint Combo 30 (NBPC 30) panel. e NBPC 30 panel was stored

at 2 to -25ºC. All antimicrobials and biochemicals were procured from

Bioeld treatment strategy

Treatment groups of each strain, in sealed pack were handed over

to Mr. Trivedi for bioeld treatment under laboratory conditions. Mr.

Trivedi provided the treatment through his energy transmission process

to the treated groups without touching the samples. e bioeld treated

samples were returned in the similar sealed condition and analyzed

on day 10 using the standard protocols. e study was conducted on

automated MicroScan Walk-Away® system (Dade Behring Inc., USA).

Evaluation of antimicrobial susceptibility assay

Antimicrobial susceptibility patterns of MDR lab isolates of K.

pneumoniae were studied using MicroScan Walk-Away® using Negative

Break Point Combo (NBPC 30) panel as per manufacturer's instructions.

e antimicrobial susceptibility pattern (S: Susceptible, I: Intermediate,

IB: Inducible β-lactamase; EBL: Suspected extended-spectrum β

-lactamases, and R: Resistant) and MIC values were determined by

observing the lowest antimicrobial concentration showing growth

inhibition [21]. e antimicrobials used in the susceptibility assay viz.

amikacin, amoxicillin/k-clavulanate, ampicillin/sulbactam, ampicillin,

aztreonam, cefazolin, cefepime, cefotaxime, cefotetan, cefoxitin,

ceazidime, ceriaxone, cefuroxime, cephalothin, chloramphenicol,

ciprooxacin, gatioxacin, gentamicin, imipenem, levooxacin,

meropenem, moxioxacin, noroxacin, nitrofurantoin piperacillin,

piperacillin/tazobactam, tetracycline, ticarcillin/K-clavulanate,

tobramycin, and trimethoprim/sulfamethoxazole

Biochemical studies of each MDR isolates of K. pneumoniae were

determined by MicroScan Walk-Away® using NBPC 30 panel system

in both control and treated groups. Biochemicals used in the study

are acetamide, adonitol, arabinose, arginine, cetrimide, cephalothin,

citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose, hydrogen

sulde, indole, inositol, kanamycin, lysine, malonate, melibiose, nitrate,

oxidation-fermentation, galactosidase, ornithine, oxidase, penicillin,

ranose, rhamnose, sorbitol, sucrose, tartarate, tryptophan deaminase,

tobramycin, urea, and Voges-Proskauer [21].

Identication by biotype number

e biotype number of each MDR isolates of K. pneumoniae in

control and treated sample were determined followed by identication

of microorganism by MicroScan Walk-Away® processed panel data

report with the help of biochemical reaction data [21].

Antimicrobial susceptibility study

Results of antimicrobial sensitivity pattern and MIC values of

control and treated MDR isolates of K. pneumoniae are summarized

in Tables 1 and 2, respectively. All these changes were observed on 10

days aer bioeld treatment as compared to control group. Overall,

46.42% of tested antimicrobials out of twenty eight, showed alteration

in antimicrobial sensitivity pattern against bioeld treated MDR

isolates of K. pneumoniae. All four MDR isolates, showed variations

in antimicrobial sensitivity assay viz. 32.14% in LS 2, 25% in LS 6,

17.85% in LS 7, and 28.57% in LS 14 against the tested antimicrobials

(Figure 1). Extended spectrum beta-lactamases (ESBLs) are rapidly

evolved group of beta-lactamases enzyme, which confer resistance to

most beta-lactam antibiotics, including penicillins, cephalosporins,

monobactam and aztreonam. Apart from beta-lactam antibiotics,

ESBLs are also resistant to other classes of non-penicillin antibiotics

[22]. Beta-lactamases are enzymes that inactivates the antibiotic and are

present in almost all Gram-negative bacilli. However, some pathogenic

production of β-lactamase which varies from low to high level. In

some species, exposure to β-lactams will induced the production level

of β-lactamase, commonly results in resistance to these agents. ese

inducible β-lactamases are frequently found in Enterobacter spp. [23].

Experimental results of antimicrobial sensitivity assay showed altered

sensitivity pattern in bioeld treated clinical isolates of K. pneumonia .

Aztreonam, cefotaxime, ceazidime, and ceriaxone sensitivity

changed from EBL → R in LS 2 and LS 14. Sensitivity of amoxicillin/k-

clavulanate changed from S → R in LS 7 and LS 14, while S → IB and

I → R in LS 2 and LS 6 respectively. Cefotetan and cefoxitin found

changed sensitivity pattern from S → R in LS 7, and while S → IB in

LS 2. Sensitivity of cefotetan changed from I → R in LS 6 while S →

IB in LS 14. Chloramphenicol and imipenem sensitivity changed from

S → R in LS 6. Although, in imipenem sensitivity changed from S →

I in LS 6. Meropenem sensitivity changed from I → R and S → R in

LS 6 and LS 7 respectively. An increase in sensitivity was reported in

piperacillin/tazobactam and piperacillin i.e. from R → I in bioeld

treated LS 6 as compared to control. Although, piperacillin/tazobactam

sensitivity changed from S → IB and S → I in LS 2 and LS 14 respectively.

Ticarcillin/K-clavulanate sensitivity altered from S → I and S → R in LS

2 and LS 14 respectively (Table 1). Rest of antimicrobials did not show

any change in sensitivity pattern aer bioeld treatment.

Determination of Minimum Inhibitory Concentration (MIC)

MIC values of all the clinical MDR isolates of control and bioeld

treated K. pneumoniae are summarized in Table 2. MIC values were

decreased in case of piperacillin/tazobactam and piperacillin in LS

6 isolate only, while in rest of the antimicrobials, MIC values were

increased as compared to control. Overall, MIC results showed an

alteration in 30% tested antimicrobials (i.e. nine out of thirty) aer

bioeld treatment in clinical isolates of K. pneumoniae (Table 2 and

Figure 1). A decreased in MIC values in piperacillin/tazobactam and

piperacillin were reported along with increases antimicrobial sensitivity

aer bioeld treatment (64 µg/ml) in LS 6. Current treatment strategy

Volume 3 * Issue 4 * 1000173

ISSN: 2329-891X JTD, an open access journal

S. No.AntimicrobialLS 2LS 6LS 7LS 14

8 CefotaximeEBLREBLEBL EBLEBLEBLR

C: Control; T: Treatment; R: Resistant; I: Intermediate; S: Susceptible; LS: Lab Isolate; EBL: Suspected extended-spectrum beta-lactamases

against K. pneumoniae infections preferably uses cefoperazone/

sulbactam, piperacillin/tazobactam, and imipenem antimicrobials

[24]. Although, piperacillin/tazobactam antimicrobial agent is useful

and preferred in neonatal infections caused due to K. pneumoniae [25].

Bioeld treatment in clinical isolate (LS 6) signicantly increased the

sensitivity and decreased the MIC values of piperacillin/tazobactam

and piperacillin. In Enterobacteriaceae family, the most prevalent

mechanism of acquired resistance in β-lactam antibiotics (piperacillin/

tazobactam and piperacillin) are the production of β-lactamases [26].

Bioeld treatment might act on enzymatic or genetic level which might

aects the β-lactamases production that may lead to alter the sensitivity

pattern of tested antimicrobials.

Biochemical and biotype number study

Biochemical study results of control and bioeld treated groups

are summarized in Table 3 and Figure 1. Results showed that overall

15.15% change in tested biochemical reactions among 4 treated MDR

clinical isolates of K. pneumoniae as compared to control. Cetrimide

changed from (-) negative to (+) positive reaction in LS 6, LS 7, and LS

14 as compared to control. Voges-Proskauer changed from (+) positive

to (-) negative reaction in LS 2, LS 6, and LS 7. Indole changed from

(+) positive to (-) negative reaction in LS 7. Ornithine changed from

negative (-) to positive (+) reaction in LS 14. Rest of biochemicals did

not show any alteration in their reaction aer bioeld treatment. Voges-

Proskauer, citrate, arabinose, lysine, glucose, sucrose, malonate are the

standard positive reaction biochemical tests of K. pneumoniae while

hydrogen sulde, indole, ornithine and cetrimide are the standard

negative reaction test. Biochemical reactions of control MDR isolates

of K. pneumoniae were well supported with literature data [27]. Based

on the biochemical results, signicant alteration in biotype numbers

were observed in all the four bioeld treated lab isolates i.e. LS 2, LS 6,

LS 7, and LS 14 as compared to control. New organism was identied

as Enterobacter aerogenes in LS 2 and LS 14 aer bioeld treatment

on day 10 with respect to control (Table 4). Bioeld treatment as

an alternate and complementary medicine, increasingly used in

biomedical health care system such as reduction in pain and anxiety

[28]. However, National Center for Complementary and Alternative

Medicine/National Institute of Health (NCCAM/NIH), now dened

bioeld therapies in subcategory of energy therapies as one of the

ve complementary medicine domain [29]. Mr. Trivedi's bioeld

treatment in pathogenic microbes were extensively studied and had

shown signicant alteration in the antimicrobial sensitivity pattern,

biochemical reactions, and biotype number [18,19]. Bioeld treatment

might be responsible to do alteration in microorganism at genetic level

and/or enzymatic level, which may act on receptor protein. While

altering receptor protein, ligand-receptor/protein interactions may

also alter that could lead to show dierent phenotypic characteristics.

Hence a cascade of intra-cellular signals may be initiated, accelerated or

inhibited [30]. e overall observations showed that, bioeld treatment

on MDR isolates of K. pneumoniae induced signicant alteration in

antimicrobial susceptibility pattern, MIC values, biochemical reactions,

# data[].attributes.attachment_link.title
Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment - PdfSR.com

# data[].attributes.attachment_link.description
Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi's biofield treatment on four MDR clinical lab isolates (LS) of K.

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		Menthol TGA Analysis- Study of Biofield Impact
	

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		Menthol TGA Analysis- Study of Biofield Impact
	

# data[].attributes.body
| Menthol' TGA analysis was conducted in the present study to determine the thermal degradation pattern of it after biofield. Visit the link for more details.

# data[].attributes.attachment_link.title
Structural and Physical Properties of Biofield Treated Thymol and Menthol | Mahendra Trivedi | Academic Room

# data[].attributes.headline
	
	
	
	




	
	
		Impact of Biofield on Thermal Stability of Thymol
	

# data[].attributes.subject
	
	
	
	




	
	
		Impact of Biofield on Thermal Stability of Thymol
	

# data[].attributes.body
The study evaluates the impact of biofield treatment on structural, crystalline and thermal stability of Thymol & Menthol (monoterpenes). Visit the link for more details.

# data[].attributes.headline
	
	
	
	




	
	
		DSC Study of Thymol - Analysis of Biofield Impact
	

# data[].attributes.subject
	
	
	
	




	
	
		DSC Study of Thymol - Analysis of Biofield Impact
	

# data[].attributes.body
The biofield treatment was studied on Themol and Menthol. The DSC analysis of Themol showed a minimal increase in melting temperature (45ºC) after the biofield energy treatment.

# data[].attributes.headline
	
	
	
	




	
	
		Menthol TGA Analysis - Evaluation of Energy Medicine
	

# data[].attributes.subject
	
	
	
	




	
	
		Menthol TGA Analysis - Evaluation of Energy Medicine
	

# data[].attributes.body
| To analyze the impact of unique energy treatment on the thermal stability of Thymol & Menthol, TGA analysis technique was used. Read here for more details.

# data[].attributes.attachment_link.title
"Structural and Physical Properties of Biofield Treated Thymol and Menthol" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Thymol and menthol are naturally occurring plant derived compounds, which have excellent pharmaceutical and antimicrobial applications. The aim of this work was to evaluate the impact of biofield energy on physical and structural characteristics of thymol and menthol.

# data[].attributes.headline
	
	
	
	




	
	
		DSC Study of Thymol & Menthol
	

# data[].attributes.subject
	
	
	
	




	
	
		DSC Study of Thymol & Menthol
	

# data[].attributes.body
| The DSC study of Thymol & Menthol was performed to evaluate the impact of human energy treatment on thermal properties of both the compounds.

# data[].attributes.headline
	
	
	
	




	
	
		Analysis of Thermal Stability of Thymol
	

# data[].attributes.subject
	
	
	
	




	
	
		Analysis of Thermal Stability of Thymol
	

# data[].attributes.body
| According to the experimental analysis after biofield energy treatment there is an increase in thermal stability of Thymol. For more details visit the link.

# data[].attributes.attachment_link.title
Structural and Physical Properties of Biofield Treated Thymol and Menthol

# data[].attributes.attachment_link.description
Thymol and menthol are naturally occurring plant derived compounds, which have excellent pharmaceutical and antimicrobial applications. The aim of this work was to evaluate the impact of biofield energy on physical and structural characteristics of thymol and menthol.

# data[].attributes.headline
	
	
	
	




	
	
		Biofield Impact on Structural Properties of Thymol
	

# data[].attributes.subject
	
	
	
	




	
	
		Biofield Impact on Structural Properties of Thymol
	

# data[].attributes.body
| It has been observed that due to the influence of Mahendra Trivedi's biofield energy treatment there is an impact on structural properties of thymol.

# data[].attributes.headline
	
	
	
	




	
	
		Energy Medicine Impact on Physical Properties of Menthol
	

# data[].attributes.subject
	
	
	
	




	
	
		Energy Medicine Impact on Physical Properties of Menthol
	

# data[].attributes.body
| The main aim of this study was to evaluate the impact of human energy treatment on structural & physical properties of menthol & thymol.

# data[].attributes.headline
	
	
	
	




	
	
		Mahendra Trivedi – Master of Transformation | Yelp
	

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		Mahendra Trivedi – Master of Transformation | Yelp
	

# data[].attributes.body
| Mahendra Trivedi has the ability to transform living and non-living objects to a higher level of potential through his Divine Energy. Know more about Mr. Trivedi by reading reviews of millions of customers on yelp.

# data[].attributes.headline
	
	
	
	




	
	
		Follow Mahendra Trivedi on LinkedIN
	

# data[].attributes.subject
	
	
	
	




	
	
		Follow Mahendra Trivedi on LinkedIN
	

# data[].attributes.body
| View Mahendra Trivedi professional profile on LinkedIn. Linkedin is the world's largest business network, helping professionals like Mahendra Trivedi. Join LinkedIn & access Mahendra Trivedi's full profile.

# data[].attributes.attachment_link.title
Mahendra Trivedi | Professional Profile | LinkedIn

# data[].attributes.attachment_link.description
View Mahendra Trivedi's profile on LinkedIn, the world's largest professional community. Mahendra has 2 jobs listed on their profile. See the complete profile on LinkedIn and discover Mahendra's connections and jobs at similar companies.

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		TGA Analysis of Biofield Treated 2,4-Dihydroxybenzophenone
	

# data[].attributes.subject
	
	
	
	




	
	
		TGA Analysis of Biofield Treated 2,4-Dihydroxybenzophenone
	

# data[].attributes.body
| The TGA analysis technique was used to evaluate the impact of Biofield energy treatment on the Thermal properties of 2,4-Dihydroxybenzophenone. Read here for more details and results.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Energy Treated 2,4-Dihydroxybenzophenone | Mahendra Trivedi | Alice Branton | Dahryn Trivedi | Gopal Nayak | Academic Room

# data[].attributes.attachment_link.description
Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of DHBP. The study was performed in two groups (control and treated).

# data[].attributes.headline
	
	
	
	




	
	
		2, 4-Dihydroxyacetophenone XRD Study - Evaluation of Biofield
	

# data[].attributes.subject
	
	
	
	




	
	
		2, 4-Dihydroxyacetophenone XRD Study - Evaluation of Biofield
	

# data[].attributes.body
| The 2, 4-Dihydroxyacetophenone XRD study showed a decrease in the volume of the unit cell and molecular weight of treated DHBP as compared to the control. Read here for more details.

# data[].attributes.attachment_link.readable_text
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Research ArticleOpen Access

Trivedi et al., Clin Pharmacol Biopharm 2015, 4:4

http://dx.doi.org/10.4172/2167-065X.1000145

Volume 4 * Issue 4 * 1000145

ISSN: 2167-065X CPB, an open access journal

Physical, Thermal and Spectral Properties of Biofield Energy Treated

2,4-Dihydroxybenzophenone

Mahendra Kumar Trivedi1, Rama Mohan Tallapragada1 , Alice Branton1 , Dahryn Trivedi1, Gopal Nayak1, Rakesh Kumar Mishra2 and Snehasis

1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA

2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India

Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of

pharmaceutical agents. The objective of this study was to investigate the inuence of bioeld energy treatment on the

physical, thermal and spectral properties of DHBP. The study was performed in two groups (control and treated). The

control group remained as untreated, and the treated group received Mr. Trivedi's bioeld energy treatment.

Methods: The control and treated DHBP samples were further characterized by X-ray diffraction (XRD), differential

scanning calorimetry (DSC), thermogravimetric analysis (TGA), laser particle size analyser, surface area analyser,

Fourier transform infrared (FT-IR) spectroscopy, and ultra violet-visible spectroscopy (UV-vis) analysis.

Results: The XRD study indicated a slight decrease in the volume of the unit cell and molecular weight of treated

DHBP as compared to the control sample. However, XRD study revealed an increase in average crystallite size of the

treated DHBP by 32.73% as compared to the control sample. The DSC characterization showed no signicant change in

the melting temperature of treated sample. The latent heat of fusion of the treated DHBP was substantially increased by

11.67% as compared to the control. However, TGA analysis showed a decrease in the maximum thermal decomposition

temperature (Tmax ) of the treated DHBP (257.66ºC) as compared to the control sample (260.93ºC). The particle size

analysis showed a substantial increase in particle size (d50 and d99) of the treated DHBP by 41% and 15.8% as compared

to the control sample. Additionally, the surface area analysis showed a decrease in surface area by 9.5% in the treated

DHBP, which was supported by the particle size results. Nevertheless, FT-IR analysis showed a downward shift of

methyl group stretch (2885→2835 cm-1 ) in the treated sample as compared to the control. The UV analysis showed a

blue shift of absorption peak 323→318 nm in the treated sample (T1) as compared to the control.

Conclusion: Altogether, the results showed signicant changes in the physical, thermal and spectral properties of

treated DHBP as compared to the control.

Keywords: 2,4-Dihydroxybenzophenone; X-ray diraction; ermal

analysis; Laser particle size analyser; Surface area analyser; Fourier

transform infrared spectroscopy; Ultra violet-visible spectroscopy.

Abbreviations: XRD: X-Ray Diraction; DSC: Dierential

Scanning Calorimetry; TGA: ermo Gravimetric Analysis; FTIR:

Fourier Transform Infrared Spectroscopy, UV-Vis: Ultra Violet-

Visible Spectroscopy Analysis; CAM: Complementary and Alternative

Benzophenone an aromatic ketone is an important class of organic

compounds used in perfumes and photochemicals. Benzophenones are

used as an intermediate for the synthesis of dyes, pesticides and drugs

[1]. ese compounds are widely used for the synthesis of various

drugs having anxiolytic, hypnotic and antihistaminic activities [2].

2,4-dihydroxybenzophenone (DHBP) is used as UV-light absorber in

resinous and polymer compositions such as polystyrene, acrylonitrile

polymer and other copolymers [3]. Moreover, these UV light absorbers

are also used in the preparation of sunscreen agents for cosmetic

applications. DHBP has been used as promising sunscreen agent that

reduces the skin damage by blocking the ultra violet light [2].

e chemical and physical stability of the pharmaceutical

compounds are most desired quality attributes that directly aect its

safety, ecacy, and shelf life [4]. Hence, it is required to explore some

new alternate approaches that could alter the physical and chemical

properties of the compounds such as DHBP. Recently bioeld energy

treatment has been used as a plausible approach for physicochemical

modication of metals [5,6], ceramic [7], organic products [8]

and pharmaceutical drugs [9]. erefore, aer considering the

pharmaceutical applications of DHBP authors planned to investigate

the inuence of bioeld energy treatment on physical, spectral and

thermal properties of DHBP.

e National Centre for Complementary and Alternative

Medicine (NCCAM), a part of the National Institute of Health (NIH),

recommends the use of Complementary and Alternative Medicine

(CAM) therapies as an alternative in the healthcare sector, and about

36% of Americans regularly uses some form of CAM [10]. CAM

includes numerous energy-healing therapies; bioeld therapy is one of

the energy medicine used worldwide to improve the health.

Fritz, has rst proposed the law of mass-energy interconversion

and aer that Einstein derived the well-known equation E=mc2 for

light and mass [11,12]. ough, conversion of mass into energy is fully

*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research Laboratory Pvt.

Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026,

Citation: Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, et al.

(2015) Physical, Thermal and Spectral Properties of Bioeld Energy Treated

2,4-Dihydroxybenzophenone. Clin Pharmacol Biopharm 4: 145. doi:10.4172/2167-

the terms of the Creative Commons Attribution License, which permits unrestricted

use, distribution, and reproduction in any medium, provided the original author and

Treated 2,4-Dihydroxybenzophenone. Clin Pharmacol Biopharm 4: 145. doi:10.4172/2167-065X.1000145

Volume 4 * Issue 4 * 1000145

ISSN: 2167-065X CPB, an open access journal

all atoms in a molecule multiplied by the Avogadro number (6.023

×1023 ).e percent change in molecular weight was calculated using

Percent change in molecular weight = [(Mt -Mc)/Mc]×100

powder sample respectively.

Percentage change in average crystallite size was calculated using

treated powder samples respectively.

Dierential scanning calorimetry (DSC)

DSC was used to investigate the melting temperature and latent

heat of fusion (∆H) of samples. e control and treated DHBP samples

were analysed using a Pyris-6 Perkin Elmer DSC at a heating rate of

10ºC/min under air atmosphere and the air was ushed at a ow rate of

5 mL/min. Predetermined amount of sample was kept in an aluminum

pan and closed with a lid. A blank aluminum pan was used as a

reference. e percentage change in latent heat of fusion was calculated

using following equations:

% change in Latent heat of fusion 100

Where, ΔH Control and ΔH Treated are the latent heat of fusion of

control and treated samples, respectively.

ermogravimetric analysis-dierential thermal analysis

e thermal stability of control and treated DHBP were analyzed

by using Mettler Toledo simultaneous TGA and Dierential thermal

analyser (DTA). e samples were heated from room temperature to

400º C with a heating rate of 5ºC/min under air atmosphere.

e average particle size and particle size distribution were

analyzed by using Sympetac Helos-BF Laser Particle Size Analyser with

a detection range of 0.1 to 875 micrometer. Average particle size d50 and

d99 (size exhibited by 99% of powder particles) were computed from

laser diraction data table. e percentage changes in d50 and d99 values

were calculated by the following formula:

Percentage Change in d99 size = 100× (d99 treated- d99 control)/ d99

e surface area of control and treated DHBP were characterized

by surface area analyser, SMART SORB 90 Brunauer-Emmett-Teller

(BET) using ASTM D 5604 method that had a detection range of

0.2-1000 m2 /g. Percent change in surface area was calculated using

% change in surface area 100

Where, S Control and S Treated are the surface area of control and treated

validated, but the inverse of this relation, i.e. energy into mass is not

yet veried scientically. Additionally, it was stated that energy exist in

various forms such as kinetic, potential, electrical, magnetic, nuclear,

etc. which have been generated from dierent sources. Similarly,

neurons that are present in the human central nervous system have the

ability to transmit the information in the form of electrical signals [13-

16]. Hence, bioeld is dened as a bioenergetic eld that permeates and

surrounds living organisms. Recently Prakash et al. reported that this

inherent biomagnetic eld around the human body can be measured

by few medical techniques such as Kirlian photography, polycontrast

interference photography and resonance eld imaging [17].

erefore, it is envisaged that human beings have the ability to

harness the energy from the environment/Universe and can transmit

into any object (living or non-living) around the Globe. e object(s)

will always receive the energy and responding in a useful manner

that is called bioeld energy. Mr. Trivedi's unique bioeld treatment

is also known as e Trivedi Eect® . It is known to transform the

characteristics of various living and non-living things. Moreover, the

bioeld treatment has caused signicant aect in dierent elds such

as agriculture [18-20] and microbiology [21-22].

e present work is focused to study the impact of Mr. Trivedi'

bioeld energy treatment on physical, thermal and spectral properties

of DHBP and characterized by XRD, DSC, TGA, particle size, surface

area, FT-IR and UV-visible spectroscopic analysis.

2,4-Dihydroxybenzophenone (DHBP) was procured from S D Fine

Chemicals Ltd, India. e sample was divided into two parts; one was

kept as a control sample while the other was subjected to Mr. Trivedi's

unique bioeld treatment and coded as treated sample. e treated

group was in sealed pack and handed over to Mr. Trivedi for bioeld

energy treatment under standard laboratory conditions. Mr. Trivedi

provided the energy treatment through his energy transmission

process to the treated group without touching the sample. e control

and treated samples were characterized by XRD, DSC, TGA, particle

size, surface area, FT-IR, and UV-visible analysis.

X-ray diraction (XRD) study

e XRD analysis of control and treated DHBP was carried out on

Phillips, Holland PW 1710 X-ray diractometer system, which had a

copper anode with nickel lter. e radiation of wavelength used by

the XRD system was 1.54056 Å. e data obtained from this XRD were

in the form of a chart of 2θ vs. intensity and a detailed table containing

peak intensity counts, d value (Å), peak width (θ°), relative intensity

(%) etc. e average crystallite size (G) was calculated by using formula:

Here, λ is the wavelength of radiation used, b is full width half-

maximum (FWHM) of peaks and k is the equipment constant (=0.94).

e molecular weight of atom was calculated using following

Molecular weight = number of protons x weight of a proton +

number of neutrons x weight of a neutron + number of electrons x

weight of an electron.

Molecular weight in g/Mol was calculated from the weights of

Treated 2,4-Dihydroxybenzophenone. Clin Pharmacol Biopharm 4: 145. doi:10.4172/2167-065X.1000145

Volume 4 * Issue 4 * 1000145

ISSN: 2167-065X CPB, an open access journal

e FT-IR spectra were recorded on Shimadzu's Fourier transform

infrared spectrometer (Japan) with the frequency range of 4000-500

cm-1 . e analysis was accomplished to evaluate the eect of bioeld

treatment at an atomic level like dipole moment, force constant and

bond strength in chemical structure [23]. e treated sample was

divided into two parts T1 and T2 for FT-IR analysis.

UV-Vis spectroscopic analysis

UV spectra of the control and treated DHBP samples were recorded

on Shimadzu UV-2400 PC series spectrophotometer with 1 cm quartz

cell and a slit width of 2.0 nm. e analysis was carried out using

wavelength in the range of 200-400 nm and methanol was used as a

solvent. e UV spectra was analysed to determine the eect of bioeld

treatment on the energy gap of highest occupied molecular orbital and

lowest unoccupied molecular orbital (HOMO-LUMO gap) [23]. e

treated sample was divided in two parts T1 and T2 for the analysis.

XRD was used to investigate the crystalline nature of the control

and treated DHBP. Figure 1 shows the XRD diractogram of the

control and treated DHBP. XRD diractogram of the control DHBP

º, 18.13 º, 18.52 º, 25.64 º, 27.93 º, 34.85 º, 37.57 º and 44.34 º. However,

15.23 º , 18.13 º, 18.50 º, 25.63 º, 27.94 º, 34.75 º, 34.85 º, 37.56 º, and 44.34 º .

e intensity of XRD peaks present at Brags angle 2θ equal to 18.13 º ,

as compared to the control. is showed an increase in crystallinity of

the treated DHBP with respect to the control sample. It is proposed

that bioeld energy treatment might increase the long-range order of

the DHBP molecules that lead to the formation of the symmetrical

crystalline pattern as compared to the untreated sample [24]. Based

on XRD peaks, control and treated samples were indexed with the

monoclinic crystal structure.

e unit cell volume, crystallite size and change in molecular

weight were computed from the XRD diractogram, and results

are presented in Table 1. e unit cell volume of control DHBP was

1063.70 10-24 × cm3 and it was minimally decreased to 1063.20 10-24 ×

cm3 in the treated sample. e treated DHBP showed a decrease in unit

cell volume by 0.05% as compared to the control sample. e molecular

weight (number of proton and neutrons) in control DHBP was 214.95

g/mol and it was minimally decreased up to 214.84 g/mol in the treated

sample. e treated sample showed 0.048 % change in molecular

weight with respect to control. It is hypothesized that bioeld energy

may be acted on the treated DHBP crystals at nuclear level and altered

the number of proton and neutrons as compared to the control, which

may lead to change in its molecular weight [25].

e crystallite size is known as a group of molecules or atoms

having the same orientation in one plane. Moreover, the crystallite

size is one of the crystallographic factors associated with the formation

of dislocations and point defects in the crystalline structure, which

directly inuences the material properties [26]. e crystallite size of the

control sample on the plane corresponding to most intense XRD peak

(i.e. 2θ = 27.9) was 78.08 nm, and it was remained unchanged in treated

DHBP sample (78.08 nm). Nevertheless, the average crystallite size was

also calculated from the XRD diractograms and data are presented

in Table 1. e average crystallite size of control DHBP was 62.19 nm,

and it was signicantly increased to 82.55 nm, in the treated sample.

e result showed an increase in average crystallite size by 32.73% in

treated sample as compared to the control. Caruntu et al. reported that

dielectric properties of barium powder could be varied by modulating

the crystallite size through an annealing process [27]. Vucinic-Vasic

et al. during their studies on zinc ferrite nanoparticles revealed that

crystallite size increases with elevation in annealing temperature [28].

Additionally, recently it was showed that introduction of ultrasound

to materials leads to substantial increase in crystallite size [29]. Hence,

it is assumed that bioeld treatment may provide waves similar like

ultrasound or thermal energy to treated DHBP atoms that led to a

decrease in dislocation densities and increase in crystallite size with

DSC study: DSC was used to investigate the melting point and

latent heat of fusion of control and treated DHBP. DSC thermogram

of the control and treated DHBP are presented in Figure 2. DSC

thermogram of the control DHBP showed a sharp melting endothermic

peak at 146.39 ºC, and it indicated the crystalline nature of the control

DHBP. However, the treated DHBP showed a minimal decrease in

melting endothermic peak and it was observed at 146.21º C.

e latent heat of fusion of control and treated samples were

obtained from respective thermograms and data are presented in

Figure 1: XRD diffractogram of control and treated 2,4-dihydroxybenzophenone.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Energy Treated 2,4-Dihydroxybenzophenone - PdfSR.com

# data[].attributes.attachment_link.description
Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of DHBP. The study was performed in two groups (control and treated).

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		Thermal Properties of DHBP after the Human Energy Treatment
	

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		Thermal Properties of DHBP after the Human Energy Treatment
	

# data[].attributes.body
| Know about how unique human energy treatment has altered the thermal properties of DHBP. DSC analysis showed an increase in the latent heat of fusion of energy treated DHBP by 11.67% as compared to the control sample.

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physical properties of 2 4-dihydroxybenzophenone

# data[].attributes.attachment_link.title
"Physical, Thermal and Spectral Properties of Biofield Energy Treated 2,4-Dihydroxybenzophenone" by Mahendra Kumar Trivedi

# data[].attributes.attachment_link.description
Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral properties of DHBP. The study was performed in two groups (control and treated).

# data[].attributes.headline
	
	
	
	




	
	
		Physical Properties of 2,4-Dihydroxybenzophenone
	

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		Physical Properties of 2,4-Dihydroxybenzophenone
	

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| The present study is based on the concept of human energy treatment. In this research work the impact of the same was studied on the Thermal, Spectral & Physical Properties of 2,4-Dihydroxybenzophenone.

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		Spectral Properties of DHBP - Human Energy Impact
	

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		Spectral Properties of DHBP - Human Energy Impact
	

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| Read here the impact of Mr. Trivedi’ biofield energy treatment on physical, thermal and spectral properties of DHBP. For more details visit the link.

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		2,4 Dihydroxybenzophenone TGA Analysis
	

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		2,4 Dihydroxybenzophenone TGA Analysis
	

# data[].attributes.body
| The 2,4 Dihydroxybenzophenone TGA analysis data shows a decrease in  maximum thermal decomposition temperature (257.66ºC) of human energy treated samples of DHBP as compared to the untreated samples.

# data[].attributes.attachment_link.title
Physical, Thermal and Spectral Properties of Biofield Energy Treated 2,4-Dihydroxybenzophenone

# data[].attributes.attachment_link.description
Study background: 2,4-Dihydroxybenzophenone (DHBP) is an organic compound used for the synthesis of pharmaceutical agents. The objective of this study was to investigate the influence of biofield energy treatment on the physical, thermal and spectral

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		2,4-Dihydroxyacetophenone XRD Study
	

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		2,4-Dihydroxyacetophenone XRD Study
	

# data[].attributes.body
| The 2 4-dihydroxyacetophenone XRD study shows increase in average crystallite size by 32.73% in human energy treated samples of DHBP.

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		Human Energy Impact on Thermal Properties of DHBP
	

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		Human Energy Impact on Thermal Properties of DHBP
	

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| Read here the thermal properties of 2,4-Dihydroxybenzophenone (DHBP) after the unique Human energy treatment.