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The peak at lower wavelengths is caused by absorbance of peptide and carboxylic acid moieties in the compounds.

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tryp 415

tyrosine : 1642
7560 total

2057 phenol

27.2%  Is phenol

do DNA binding proteins usually have a higher percentage of phenol amino acids?

what percentage of the complex is DNA?

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How phenol affects absorbance ratios

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260 is for DNA, 280 is for proteins

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Paper E uses fungus Chaetomium thermophilum and has higher definition

Paper A uses human complex

Paper E has acces to way more protein, and you need to average the data from all the protein, therefore higher resolution

Fungus is easier to purify, therefore will have to go through fewer steps of purification, and will be better quality

Also its heat stable, and recombinant protein with conserved subunits between yeast and humans

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thermophilum is more stable
paper E - model building and refinement was done iteratively from already known high resolution homology models

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Lydia's answer:
"ADP.BeF₃is an ATP analog, the binding of ADP.BeF₃to ATPase receiver domain to ensure ATPase remains in the ground state and cannot undergo hydrolysis. MST is microscale thermophoresis which monitors changes in the hydration shell in conditions close to that in the cell. The data suggest it is useful to demonstrate the similarity of cooperativity in ADP.BeF₃being almost the same as that of the apo enzyme with both values being 1.6. However ADP.BeF₃has a significantly higher binding affinity than the apoenzyme as it requires a lower concentration, approximately 42nM less, for ½ of the substrate to bind suggesting that whilst ADP.BeF₃bound INO80 is less active than INO80 it still is more reactive that the apoenzyme.

https://doi.org/10.1016/j.str.2007.02.007

https://www.biozentrum.unibas.ch/uploads/media/Microscale_Thermophoresis_Intro.pdf
"

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706439/

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Dissociation constant: when half the protein in bound

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ADP.BeF3 tightens nucleosome binding.

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2 DNA chains and 10 protein chains