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Third Murderer: There's but one down. The son is fled.

Banquo (Max) is murdered by an assassin (Sailor) hired by Macbeth, while his son, Fleance (Ellie), escapes (photo cred Mary).

This is a crucial turning point in the play because it leads to  confirmation of the Witches' earlier prophecy of Banquo's heirs becoming kings.

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Banquo: "O treachery! Fly, good Fleance, fly, fly, fly!
Thou mayst revenge--O slave! (25-26)
This scene complicates Macbeth's plan because it ensures that Banquo has an issue who can be king. Macbeth had intended to kill both Banquo and Fleance but Fleance escapes.
Players:
Murderer: Shelby
Banquo: Emily
Fleance: Stacey

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"fly, good Fleance, fly, fly, Fly!

Fleance (Hanna)  is fleeing the murderer (Andrew) who is gloating over killing Banquo (Jenna).

It is significant because it allows Fleance to take revenge/become the king.  Also it's the only death in the act.