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Reliable, consistent, and sustainable synthetic cells help to standardize your research.

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Fluorescence experiment design platform — Search. Find. Design. Our tools simplify complex experiment design, save time, & reduce errors.

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Aurora Training Video (University of Chicago)

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Aurora Training Video (University of Chicago)

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Cytek Aurora Training Part 1: Introduction to Full Spectrum Flow Cytometry

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2 minute video on Compensation (part II)

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2 minute video on Compensation (part II)

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The most complete fluorescent labeling and detection reference available, The Molecular Probes Handbook-A Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 reagents and kits representing a wide range of Invitrogen Molecular Probes labeling and detection products. The significantly revised 11th edition features extensive references, reorganized content, and new technical notes and product highlights.

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guide to fluorescent probes labeling technologies

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The Molecular Probes Handbook | Thermo Fisher Scientific - US

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The most complete fluorescent labeling and detection reference available, The Molecular Probes Handbook-A Guide to Fluorescent Probes and Labeling Technologies contains over 3,000 reagents and kits representing a wide range of Invitrogen Molecular Probes labeling and detection products. The significantly revised 11th edition features extensive references, reorganized content, and new technical notes and product highlights.

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Impact of adjusting voltages on spillover and compensation

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Impact of adjusting voltages on spillover and compensation

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The impact of adjusting PMT voltages on spillover and compensation

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Measurement of Extracellular Vesicles and Other Submicron Size Particles by Flow Cytometry: Cytometry Part A: Vol 89, No 2

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Flow Cytometry Introduction - Malte Paulsen (EMBL)

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Imaging Flow Cytometry: A Brief Overview - Andrew Filby (Newcastle U.)

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Cookies are disabled for this browser. Wiley Online Library requires cookies for authentication and use of other site features; therefore, cookies must be enabled to browse the site. Detailed information on how Wiley uses cookies can be found in our Privacy Policy.

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Cookies are disabled for this browser. Wiley Online Library requires cookies for authentication and use of other site features; therefore, cookies must be enabled to browse the site. Detailed information on how Wiley uses cookies can be found in our Privacy Policy.

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Chromocyte: Antibody research tool, panel design and education center

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Chromocyte: Antibody research tool, panel design and education center

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Welcome to Chromocyte, the website by flow cytometrists, for flow cytometrists. Chromocyte is a free to use flow cytometry resource for scientists. Within this website is everything flow cytometry related, from beginners guides, , instruments, conferences and flow cytometry training courses to core facility resources and . Whether just launching a career in flow cytometry or an experienced user looking for a new challenge, our flow cytometry jobs website is the only website dedicated to positions in this area.

Designing multiplex flow cytometry assays is an extremely complex task, our panel builder guides you step-by-step along this complicated process. Not only ensuring compatibility with your instrument and lasers, but also suggesting compatible fluorochromes and sourcing antibody suppliers. In addition to the panel builder, a searchable antibody database helps you locate antibody or isotype control suppliers. Got a flow cytometry question? Access our online research community to get the answers or share your knowledge.

For the latest innovations in flow cytometry see our news and blog sections, and access our articles, videos and webinars. Plus, don't forget to subscribe to our E-Newsletter or our regularly updated Flow Cytometry News.

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Flow Cytometry Antibodies & Reagents, Antibody Search

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Welcome to Chromocyte, the website by flow cytometrists, for flow cytometrists. Chromocyte is a free to use flow cytometry resource for scientists. Within this website is everything flow cytometry related, from beginners guides , , instruments, conferences and flow cytometry training courses to core facility resources and .

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International Society for the Advancement of Cytometry

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International Society for the Advancement of Cytometry

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International Society for Advancement of Cytometry

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Students are key members of our Society. ISAC offers support for attendance at congresses, and supports a variety of associated meetings and courses. We also encourage oral and poster presentations at CYTO, our annual congress. You can be sure to receive a quality discussion on your presentation - our members care for the next generation of leaders.

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Welcome to the Purdue PUCL Cytometry discussion. Established in 1989, the list has been continuously available for free access to all scientists. The current membership is approximately 4500 scientists.

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Welcome to the Purdue PUCL Cytometry discussion. Established in 1989, the list has been continuously available for free access to all scientists. The current membership is approximately 4500 scientists.

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Information about individual fluorophores

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At BioLegend, we've spent nearly two decades crafting new fluorophores for flow cytometry. With each new option, researchers and colleagues have come to trust the performance of our products, as evidenced by this CiteAb report indicating we are the most cited company for flow cytometry antibodies.

Our diverse array of fluorophores allows you to expand the limits of flow cytometry and develop larger multicolor panels. High level panels (>22 colors) can be built using cytometers capable of spectral detection or the ability to unmix a fluorescent spectrum across an array of emission detectors such as the Cytek™ Aurora or Northern Lights. Some fluorophores, like the Spark dyes, are specifically designed to be used on these cytometers.

Learn more about the unique advantages offered by each member among the various families of fluorophores and how to best use them in multicolor panels:

Protein-based fluors: PE, APC, PerCP, and their tandems

Simple organic fluors: FITC, Alexa Fluor® dyes, Spark Fluors, etc.

Organic polymers: Brilliant Violet™ Dyes

KIRAVIA multimers: KIRAVIA Dyes™, a distinct class of multimers with unique fluorescent chemistry.

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At BioLegend, we've spent nearly two decades crafting new fluorophores for flow cytometry. With each new option, researchers and colleagues have come to trust the performance of our products, as evidenced by this CiteAb report indicating we are the most cited company for flow cytometry antibodies.

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OMIPs: Optimised Multicolour Immunofluorescence Panel

If you have spent months thoroughly optimising you antibody panel and its unique features would be beneficial to your field, consider submitting your work as a peer-reviewed publication as an OMIP. As well as having your work recognized you also help facilitate coherence within your field of research by publishing your panel. Here are some links for how you might publish your Optimised Multicolour Immunofluorescence Panel 1 2

List of available OMIPs:
OMIP‐001: Quality and phenotype of Ag‐responsive human T‐cells OMIP‐002: Phenotypic analysis of specific human CD8+ T‐cells using peptide‐MHC class I multimers for any of four epitopes OMIP‐003: Phenotypic analysis of human memory B cells OMIP‐004: In‐depth characterization of human T regulatory cells OMIP‐005: Quality and phenotype of antigen‐responsive rhesus macaque T cells OMIP‐006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry OMIP‐007: Phenotypic analysis of human natural killer cells OMIP‐008: Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells OMIP‐009: Characterization of antigen‐specific human T‐cells OMIP‐010: A new 10‐color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples OMIP‐011: Characterization of circulating endothelial cells (CECs) in peripheral blood OMIP‐012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice OMIP-013 Differentiation of Human T cells OMIP‐014: Validated multifunctional characterization of antigen‐specific human T cells by intracellular cytokine staining OMIP‐015: Human regulatory and activated T‐cells without intracellular staining OMIP‐016: Characterization of antigen‐responsive macaque and human T‐cells OMIP‐017: Human CD4+ helper T‐cell subsets including follicular helper cells OMIP-018 Chemokine receptor expression on human T helper cells OMIP-019 Human gamma delta T cells, iNKT cells, Hematopoietic Precursors OMIP-020 Phenotypic characterisation of human gamma-delta T-cells by multicolor flow cytometry OMIP-021 Simultaneous quantification of human conventional and innate-like T-cell subsets OMIP-022 Comprehensive Assessment of Antigen-Specific Human T-Cell Functionality and Memory OMIP-023 10‐Color, 13 antibody panel for in‐depth phenotyping of human peripheral blood leukocytes OMIP-024 Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples OMIP-025: Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining OMIP-026: Phenotypic analysis of B and plasma cells in rhesus macaques OMIP-027: Functional analysis of human natural killer cells OMIP-028: Activation panel for Rhesus macaque NK cell subsets OMIP-029: Human NK-cell phenotypization OMIP-030: Characterization of human T cell subsets via surface markers OMIP-031: Immunologic checkpoint expression on murine effector and memory T-cell subsets OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland OMIP-033: A comprehensive single step staining protocol for human T- and B-cell subsets OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies OMIP-035: Functional analysis of natural killer cell subsets in macaques OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets OMIP-038: Innate immune assessment with a 14 color flow cytometry panel OMIP-039: Detection and analysis of human adaptive NKG2C+ natural killer cells OMIP-040: Optimized gating of human prostate cellular subpopulations OMIP-041: Optimized multicolor immunofluorescence panel rat microglial staining protocol OMIP-042: 21‐color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood OMIP-043: Identification of human antibody secreting cell subsets OMIP-044: 28‐color immunophenotyping of the human dendritic cell compartment OMIP-045: Characterizing human head and neck tumors and cancer cell lines with mass cytometry OMIP-046: Characterization of invariant T cell subset activation in humans OMIP-047: High‐Dimensional phenotypic characterization of B cells OMIP-048: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms OMIP‐050: A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules OMIP‐051 - 28‐color flow cytometry panel to characterize B cells and myeloid cells OMIP‐052: An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques OMIP‐053: Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg Subsets OMIP‐054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry OMIP‐055: Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood OMIP‐056: Evaluation of Human Conventional T Cells, Donor‐Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining OMIP‐057: Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry Panel OMIP‐058: 30‐Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells OMIP‐059: Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining Panel OMIP‐060-30‐Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T Cells OMIP‐061: 20‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting Cells

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OMIPs: Optimised Multicolour Immunofluorescence Panel If you have spent months thoroughly optimising you antibody panel and its unique features would be beneficial to your field, consider submitting your work as a peer-reviewed publication as an OMIP. As well as having your work recognized you also help facilitate coherence within your field of research by publishing your panel.

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Don't see a fluorochrome you use in the list above, or have other suggestions? Let us know.

cFluor is a trademark of Cytek Biosciences. APC/Fire, PE/Dazzle, Spark Blue, Zombie Aqua, Zombie Green, Zombie Red, Zombie Violet, Zombie Yellow, Zombie NIR, and PE/Fire are trademarks and property of BioLegend, Inc. "BV" stands for Brilliant Violet™, which is a trademark of Sirigen Group Ltd. BV480, BB515, BB700 and APC-R700 are part of the BD Horizon™ brand, which is a trademark of BD Biosciences. BUV395, BUV496, BUV563, BUV661, BUV737, BUV805, and PE-CF594 are part of the BD OptiBuild™ and BD Horizon™ Brands, which are registered trademarks of BD Biosciences. APC-H7 is part of the BD Pharmingen™ brand, which is a trademark of BD Biosciences. Alexa Fluor, eFluor, LIVE/DEAD, DyLight, CellTrace, CellTracker, MitoSOX, and MitoTracker are trademarks of Thermo Fisher Scientific. Qdot is a registered trademark of Thermo Fisher Scientific. Cy and CyDye are registered trademarks of GE Healthcare. Vio is a registered trademark of Miltenyi Biotec GmbH. DRAQ5 and DRAQ7 are registered trademarks of Biostatus Limited. CF, Live-or-Dye, and NucView are registered trademarks of Biotium, Inc. Ghost Dye, redFluor, and violetFluor are trademarks of Tonbo Biosciences. NovaBlue and NovaYellow are registered trademarks of Phitonex, Inc. Krome Orange is a trademark of Beckman Coulter. Northern Lights is a registered trademark of R&D Systems. Janelia Fluor is a registered trademark of Tocris Bioscience. KIRAVIA Dyes™ and KIRAVIA Blue 520™ are trademarks of SONY Corporation. Trademarks are the property of their respective owners. Allophycocyanin (APC) conjugates: US Patent No. 5,714,386 PE-Cy7: US Patent No. 4,542,104 APC-Cy7: US Patent No. 5,714,386. DsRed and Fruit Fluorescent Proteins (e.g. mCherry): U.S. Patent No. 7,005,511; 7,157,566; 7,393,923 and 7,250,298.

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Practical Flow Cytometry - Beckman Coulter

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Beckman Coulter Life Sciences is taking actions in the best interests of our associates, customers, and business partners as we navigate the growing threats of the 2019 Novel Coronavirus disease (COVID-19). Learn more Dismiss Beckman Coulter is proud to make available Howard M. Shapiro, M.D., P.C.'s Practical Flow Cytometry, 4th Ed.