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How should I reference the lab book? What should I put for the author, year etc.

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How should I reference the lab book? What should I put for the author, year etc.

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Whats the point of the equation if we just follow the video to calculate IC50. Can we just use the equation and would x be 50?

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Whats the point of the equation if we just follow the video to calculate IC50. Can we just use the equation and would x be 50?

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Do we need to describe the process of preparing agarose gel in method?

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in the introduction do we mention the 3 cell death pathways but then state that it was this specific pathway that was discovered?

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in the introduction do we mention the 3 cell death pathways but then state that it was this specific pathway that was discovered?

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I’m a bit confused by what IC75 means. Is it the drug concentration where cell viability is 75% or when 75% of cells have died (i.e. cell viability is 25%)?

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I’m a bit confused by what IC75 means. Is it the drug concentration where cell viability is 75% or when 75% of cells have died (i.e. cell viability is 25%)?

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Should the whole paper be written in past tense passive voice?

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is it ok to reference scientific articles that are from the 2000s or 2010s or are those too old?

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is it ok to reference scientific articles that are from the 2000s or 2010s or are those too old?

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The Guidance on the canvas does not tell me the volume of IC50 of the drug diluted in growth media and how to make the negative control (untreated cells). How can I figure out them?

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Do I need to reference the methods

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how do i reference the handbook??

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Is it okay to use sources that arent scientific articles like NHS websites or articles written by people on websites?

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Does the electrophoresis NOT tell you if its necrosis or necroptosis? I know it isnt apoptosis

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Does the electrophoresis NOT tell you if its necrosis or necroptosis? I know it isnt apoptosis

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What are the units for IC50

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What are the units for the drug used

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Do we use the cell viability graph to work out the ic50 or the OD graph?

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For the OD data plot are we doing the average of each column or finding the average for every student group

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which dataset do we use to calculate IC50?

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how do we include standard deviation bars in our graphs when were just plotting the singular mean values against cell viability?

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What does KH61220 refer to? Is this the derivative of Naphthyridine?

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Would I link The cell count viability experiment we did under the microscope with the MTT assay ?

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Would I link The cell count viability experiment we did under the microscope with the MTT assay ?

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What figure do we do for this?

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How do they all link together?

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Can we add a picture of the actual electrophoresis gel 

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Can we add a picture of the actual electrophoresis gel 

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Should we include 3 different subheadings for each test in the methods and results session? 

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Should we include 3 different subheadings for each test in the methods and results session? 

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Do we have to do a methods for MTT Assay, im uno fluorescence test, electrophoresis AND our cell count viability under the microscope?

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I don’t understand what the data is that we have been given, what are the numbers and where have they been calculated from 

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I don’t understand what the data is that we have been given, what are the numbers and where have they been calculated from 

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SG58008 - PBS OD is 0.71 and the OD for 0.063mM is 0.718 so the % viability is greater than 100%

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SG58008 - PBS OD is 0.71 and the OD for 0.063mM is 0.718 so the % viability is greater than 100%

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Should we include 3 different subheadings for each test in the methods and results session? 

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Should we include 3 different subheadings for each test in the methods and results session? 

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do we have to go into detail on the methods of this?

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do we have to go into detail on the methods of this?

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Do we use statistical tests such as correlation coefficient to further our conclusion or not?

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For the % cell viability, do we keep values over 100 in?

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For the % cell viability, do we keep values over 100 in?

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What is the protein?

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when explaining with what concentration we have treated the 2 DNA samples should we use IC50 and IC75 or should we put specific values

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i know the FAQ said we don’t have to include a hypothesis if we’ve expanded on the aim of the study but if we do include hypotheses should we stick to one? or could we expand and come up with two for example

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why do we only get till monday to do this assignment it seems like a lot to do in such a small amount of time?

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This is probably a silly question as I know methods aren't referenced however are we expected to reference the lab book as this is where we "got" the methods from? Asking because we're expected to reference R if using it is mentioned in the methods :)

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do we add a picture of our gel to the lab report? or just desribe/analyse

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do we add a picture of our gel to the lab report? or just desribe/analyse

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The lab book said purple formazan was quantified at 550-570 nm but the MTT assay sheet says something about OD readings (data prior to normalisation) being read at 570nm. could you please explain this?

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I can see the MTT data and MLKL data on the excel sheet but don’t understand which part of the spreadsheet is the electrophoresis dataset

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What kind of graphs do we need for mtt assay

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I don’t know how to write a method on the immunofluorescence part when it’s not what we did in the lab. Even with the help material

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do we have to reference the manual

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Is the mean being calculated for each drug concentration columns or is it for each group’s drug concentrations?

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What are MLKL Clusters where can i find more about them

What does this protein do

How do we used this to determine the pathway to focus our lab report about ?

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How many graphs do we need

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At what point in the method should the chamber be removed from the slide?

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Are we plotting the mean for each drug concentration for each lab group or means including all of the groups results

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Are we plotting the mean for each drug concentration for each lab group or means including all of the groups results

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Do we need to perform statistical analysis on this data too or just immunofluoroesnce?

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Around how many words should the total method section be?

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What are we actually investigating

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I've calculated the SDs for the data but on google sheets i cant add custom standard deviations

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How do you add error bars to a graph that is showing % viability? Do you have to calculate the standard deviation into a percentage?

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What’s the hypothesis?

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Do we need to perform a stats test on these results??

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how many statistical analysis are  recommended ? 

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how many statistical analysis are  recommended ? 

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Does normalising the data mean to just compare it to the control?

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Does normalising the data mean to just compare it to the control?

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Do we need to calculate IC50

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What do you mean by plotting the data as raw OD values?

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What do you mean by plotting the data as raw OD values?

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Why does the IC50 lane shows a more intensive smear than IC75? Does IC75 even produce a smear and if not why?