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THE CENTRIFUGE

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TOUCH this image to discover its story. Image tagging powered by ThingLink

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Gel electrophoresis

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A restriction enzyme is added to 4 DNA samples (one took from the hypothetical crime scene and three from the suspects).
Image by Tom Mooring
LAB REPORT : GEL ELECTROPHORESIS
ELECTROPHORESIS
AGAROSE GEL PREPARATION
DIGESTION
Samples of DNA with restriction enzyme are put in a warm bath at 37° (the correct temperature for the digestion) for 45 minutes.
The enzyme cuts the DNA into smaller fragments.
(video)
To prepare an agarose gel we have to:

dilute 10 ml of TBE solution (which is concentrated five times more than needed) with 40 ml of water;

weigh 0.4g of agarose using a scales;

dissolve the agarose in the buffer putting them into the microwave oven for two minutes;

pour the solution in a gel tray (where is located a comb to create the wells), making sure there aren't air bubbles;

let it thicken for 40 minutes.

The gel tray is placed in an electrophoresis chamber filled with buffer. This allows the electric current to flow through the gel.
DNA samples are mixed with a 'loading dye' (composed by glycerol, bromophenol blue and xylen cyanol) to make the fragments visible.
The wells of the gel tray are filled with the DNA samples mixed up with the loading dye.
(video)
The negative and the positive electrodes of the electrophoreris chamber are attached to a power supply.
The DNA fragments move through the gel towards the positive electrode: the larger ones move slower than the smaller ones, so bigger fragments remain near to wells.
RESULTS & COMPARISON
C 1 2 3
The pattern equal to the sample C is culprit's : in this case it's #2

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A restriction enzyme is added to 4 DNA samples (one took from the hypothetical crime scene and three from the suspects). Image by Tom Mooring LAB REPORT : GEL ELECTROPHORESIS ELECTROPHORESISAGAROSE GEL PREPARATION DIGESTION Samples of DNA with restriction enzyme are put in a warm bath at 37° (the correct temperature for the digestion) for 45 minutes.

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Short Tandem Repeats & Restriction Fragment Length Polymorphism