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Assalamualaikum Dr. , maafkan kami sebab lambat hantar kerana terdapat masalah untuk menghantar. 

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Assalamualaikum Dr. , maafkan kami sebab lambat hantar kerana terdapat masalah untuk menghantar. 

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BMS424 THE SIMILIRATIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES.

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Dr., we are very sorry for sending late.

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Dr., we are very sorry for sending late.

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we have trouble to find this page

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Blended learning 27March AS2442A

Sorry dr, a bit late. Had some problem uploading it. Thankyou.

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BMS424 ASSIGNMENT BLANDED LEARNING

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BLENDED LEARNING: DIFFERENCES AND SIMILARITIES IN REPLICATION AND TRANSCRIPTIOO

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BLENDED LEARNING: DIFFERENCES AND SIMILARITIES IN REPLICATION AND TRANSCRIPTIOO

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Members:
NURIN YASMIN BINTI MOHD KHAIRUDIN (2018802242)
NUR AZLIN BINTI HELME  (2018440072)
NUR AFIFAH BINTI SOFIAN (2018659588)
NURUL ATIQAH BINTI KHAIROL ANUAR (2018439024)

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BMS424 BLENDED LEARNING AS2442A

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The similarities and differences between transcription and translation in prokaryotes

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AS2442A
BMS424 ASSIGNMENT 1

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NUR SYIFA AUNI MHD NAIM 2019554967
NUR NINA ARINA BT AHMAD AZMI 2019554715
SITI NOOR LAILI BT AB WAHID 2019317221

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Similarities and Differences of Replication and Transcription of Prokaryotes

Members:
1. Muhammad Zafri bin Zamri (2018654202)
2. Muhammad Nor Akmal bin Otsman (2018414594)
3. Amirul Akmal bin Zulkifli (2018272936)
4. Muhammad Amirul Amin bin Che Mohd Husin (2018695784)

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Assignment BMS424
AS2442A

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Transcription and Replication of Prokaryote

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Blended learning AS2442A (DNA replication and transcription)

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BLENDED LEARNING  (AS2442A) 27/3/19 WED

1. RAJINA BT SHAHMIR SIVARAJ 2018276304

2. NURUL ATIKAH BINTI BADRUL HISHAM
2018410802

3. DEFIZZATI SOFIA BINTI DEFRIJAL
2018679792

4 NURUL IZZATI BINTI AHMAD FADZULI 2018435378

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1)      Muhammad Amirularif Bin Mohd Akhirudin (2019313529)

2)      Muhammad Shamsuzzaman Bin Ilham Fitri (2019570651)

3)      Muhammd Soleh Bin Kamarulzaman (2018292358)

4)      Muhammad Luqmanulhakim Bin Aidil (2019705045)

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similarities and differences between transcription and replication in prokaryote.

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similarities and differences between transcription and replication in prokaryote.

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1. SITI NUR MAHIRAH BT MAZNAAN (2019707317)
2. SITI NURHANISAH BT MOHD PAKARI (2019583851)
3. IZZATTUL AFEEQAH BT ABDUL RAZAK (2019351143)
4. NIK NUR AZWARINA BT R.AZMI (2019329003)

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AS2442B
1)AININ SOFFIYA BINTI MUHAMMAD SABRI
2)NOR IFFAH SYAHIRAH BINTI SAHARU

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BMS424 Similarities and Differences between Replication and Transcription in Prokaryote

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AS2442B

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AUFA AMATULLAH BINTI MOHD YAZID
AIN NAJIHAH BINTI SOFIAN
SITI HAJAR BINTI MOHD RASADI
RIDZHA IZZUANI BINTI MUHAMMAD RIDZUAN NG

# data[].attributes.body
AISYAH BINTI ISMAIL (2019728237)
NUR ARINA IZZATI BINTI ARMAN (2019314513)

NOR KHAIRIYAH BINTI ZAMRI (2019336647)
NUR FATIHAH NAJIHA BINTI AZHAR (2019345893)

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SIMILARITIES AND DIFFERENCES BETWEEN REPLICATION AND TRANSCRIPTION PROCESS IN PROKARYOTE

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES 

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES 

# data[].attributes.body
1. NUR ALIFAH ILYANA BT MOHD NAIM (2019722637)
2. NUR AFIQAH BT CHE AZIZ (2019324645)
3. NURFARHANA BT KAMARUDIN (2019361629)
4. FELICIA VANOOSTEN ANAK STEPHEN JINUN (2019316987)

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES

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SIMILARITIES AND DIFFERENCES OF TRANSCRIPTION AND REPLICATION IN PROKARYOTES

# data[].attributes.body
-          NUR ATHIRAH NABILAH BINTI MOHD KHAIRI (2019582371)

-          NUR AQILAH BINTI MOHAMAD RADZI (2019707221)

-          NURUL HAFIZA BINTI HJ. MOHD NAJIB (2019726035)

-          SYAHIDATUL FARAHUSNA BINTI MAZLAN (2019163229)

# data[].attributes.body
Khairina Nabihah Binti Mah Yudin (2019707613)
Fathihah Athasya Binti Zainol Abidin (2019725947)
Nur Liyana Binti Razali (2019725331)
Nor Atikah Binti Hussain (2019725487)
Muhammad Afif Bin Mah Hassan (2019717819)

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Awak

# data[].attributes.body
Farah Nurafiqah bt Mohamad Faizal (2019304021)
Ainin Sofiya bt Azrulhisham (2019994129)
Siti Halawati bt Kamarudin (2019764413

# data[].attributes.body
Nuramira Syafiqa(2017318287)
Nurul Hamiemah(2017377683)
Nurun Nusa'(2017522799)
Lutfia ilsahaida(2017593611)

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1. Muhamad Syazwan B. Fazale (2017726081)

2.Fauzan Haziq B. Mardin (2017148657)

3. Muhammad Hasni B. Rosli (2017134357)

4.Ika Cemalia Bt. Ahmad Rohimin (2017501647)

# data[].attributes.body
SYED ZAIN BIN SYED IDROS
(2017327219)
MUHAMAD DANIAL BIN MOHAMED
(2017773721)
FADHIL BIN AHMAD
(2017119147)
Ahmad Hilmi Haziq bin Abd Hamid
(2017711963)

# data[].attributes.body
1. HAZEEYAH NADHEERAH BINTI ZAKARIA (2017948271)
2. NURFARAHAIN BINTI MOHAMAD FAUZI (2017369837)
3. NURIN NAZIRAH BINTI MOKHTAR (2017978745)
4. NURUL NADIA BINTI KAIRUDDIN (2017134235)
5. SITI NUR AISYA BINTI IBRAHIM
(2017197343)

# data[].attributes.body
NUR FATIN AFIQAH BINTI NOR RAHIM (2017917491)
NORHIDAYU BINTI ROSLI (2017951841)
WAN NURIN AFIQEEN BINTI WAN MAIZUL KHAR (2017702491)
MICHEAL ALLEN ANAK MARY (2017988489)

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Amal Nurnadia Razali (2017447918)
Anis Syahirah Saifor Adzuan (2017447914)
Nurul Amirah Binti Rosdi (2017656792)
Ratu Mas Kinaz Binti Norlan (2017656794)

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1. Asmaa' Zuhaira Binti Asri
(2017447902)
2. Nurzahra Binti Amin Nasir
(2017447898)
3. Anis Ilyani Binti Kamarruddin
(2017656758)
4. Muhammad Faris Shazwan Bin Shariff
(2017447908)

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1. Abdul Haziq Ikhwan bin Abdul Halid Han (2017583241)
2. Wan Alif Afiq bin Wan Nor Ruddin (2017776423)
3. Aiman Haiqal bin Azhari (2017381755)
4. Khairul Anwar bin Ahmad (2017119683)

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Watch the video: BMS by haziqrock on Vyond.

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1. WAN NUR SYAMIMI BINTI RAHIMI (2017987915)
2. ZULAIKHA HAZIQAH BINTI MOHAMAD ZULKIFLI (2017947093)
3. SITI NOR SUHAILAH BINTI MAT SAAT (20179)
72081)
4. NOR SYAFIKAH BINTI MOHD NASIR (2017982441)

Don't forget to vote for us! xoxo :))

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1. KAMALEA AMALEENA FARHANA BINTI KAMAL RAMLEE (2017533503)
2. NIK FATIN FARISYA BINTI HARUN (2017981947)
3.PRISCILLIA DURRIE ANAK DASAN (2017921981)
4. SITI ZULAIKHA BINTI MAKHDAR (2017706727)

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1. Wan Nurhazirah Fatini bt Wan Hassan
2017656796
2. Nur izyan 'izzati bt zahran
2017656772
3. Nurain Sidiiqin Binti Mohamad Arbi
2017656806
4. Nurul Syuhada Binti Yushaza
2017447906
5. Muhammad Firas Hidayat Bin Mohd Yusoff
2017656802

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Fun Facts About Genetics
1. Nurul Amirah (2017921391)
2. Nur Atikah Hazirah (2017745514)
3. Siti Nur Aminara (2017153275)
4. Rohadatul Aisy (2017172273)

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1. Ainnur shafirah binti che ishak (2017173693)
2.Noor fahanna binti zaidan (2017787463)
3. Raja fairuz shafizah bt raja mohamad aznan (2017997131)
4.Intan Nazyra Farhana Binti Abdul Rashid (2017105275)
5.Sofia Zaireen Bt Shahrizman ( 2017160771)

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SIGENES ( FUN FACTS ABOUT GENETICS)

1.  SITI ZULAIKHA BINTI ZULKEFELI
-2017656804
2. NAJWATUL ALIYA BINTI SABRAN
-2017656788
3. AMIRAH HANANI BINTI ABDUL ZAKI
-2017447916
4. NUR NAJIHAH BINTI NASUHA
-2017447912
5. RAJA NUR FAQIHAH BINTI RAJA SHARIN
-2017447894

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NUR SHAFIQAH BT MAT ROFII (2017132381)
NISA' MARINI BT ANDUL JAMIL (2017337959)
NURUL NADIAH BT ISMAIL (2017143103)
AZYAN ANISAH BT RAMLI (2017998411)

2 FUN FACTS ABOUT GENETICS.

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1. Ain Nur Najihah Bt Muhd Juhari
(2017301321)
2. Nurul Farhana Bt Md Noor
(2017507575)
3. Farah Hani Filzah Bt Fajim Juffa
(2017970591)
4. Nur Azila Suhana Bt Mat Yunos
(2017726587)
5. Nurhidayatul Nazuha Bt Zamri
(2017735175)

# data[].attributes.body
NUR SHAFIQAH BT MAT ROFII (2017132381)
NISA' MARINI BT ABDUL JAMIL (2017337959)
NURUL NADIAH BT ISMAIL (2017143103)
AZYAN ANISAH BT RAMLI (2017998411)

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Types of ribosomes

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Michael Allen 2017988489
Wan Nurin Afiqeen Bt Wan Maizul Khar 2017702491
Nur Fatin Afiqah Bt Nor Rahim 2017917491
Norhidayu bt Rosl2017951841

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Muhammad Hasni
Fauzan Haziq
Muhamad Syazwan
Haziq
Alif
Khai
Aiman

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Class Attendance 4 A
 Class Attendance 4 April 2018


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Assignment 1:
Assignment 1:
1. Nur Shafiqah bt Mat Rofii 
(2017132381)
2. Azyan Anisah Binti Ramli (2017998411)
3. Nurul Nadiah Binti Ismail (2017143103)
5. Nisa' Marini binti Abdul Jamil (2017337959)


1) PRIMER sequence: 5’ to 3’ and 3’ to 5’ sequence ( A sequence that complementary to the template) 
2) Type of DNA sample: Bacterial DNA
3) Melting Temperature: 95 degree celcius.
4) Commercial name for DNA polymerase used: Taq DNA Polymerase
5) How many cycle of replication: can be up to 40 cycle. 

 

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ASSIGNMENT 1
ASSIGNMENT 1 
ABDUL HAZIQ IKHWAN BIN ABD HALID HAN- 2017583241
KHAIRUL ANWAR BIN AHMAD-2017119683
AIMAN HAIQAL BIN AZHARI-2017381755
WAN ALIF AFIQ BIN WAN NOR RUDDIN-2017776423

PCR
1. Forward primer and reverse primer, 5’ to 3’
2.  DNA of honey bee and mantis 
3. 54.5-59.5 °C
4. Taq polymerase
5.  30 cycles 
 

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ASSIGNMENT 1
ASSIGNMENT 1
WAN NUR SYAMIMI 2017987915
ZULAIKHA HAZIQAH BINTI MOHD ZULKIFLI 2017947093
NOR SYAFIKAH BINTI MOHD NASIR 2017982441SITI NOR SUHAILAH BINTI MAT SAAT 2017972081

1. DNA primer sequence - refer Table 1.
2. DNA sample - Genes in Gossypium hirusutum
3. Commercial name - Platinum Taq DNA polymerase (Invitrogen)
4. Replication sample - 40 cycle of 15s at 94Celcius, 10s at 60Celcius and 15s at 72 Celcius
5. Melting point - 60 Celcius
 

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Nur atikah hazirah bt zarimi (2017745517) 
Rohadatul aisy binti zolmi (2017172273) 
Siti nur aminara binti mohd azlan (2017153275) 
Nurul amirah binti mohamad farook (2017921391) 
 
 
1)      DNA primer sequence- Standard primer PCR reactions were performed using the c and f primers (5′-GCTCAGCTCCGTTTCGGTTTCACTTCCGGT-3′; 5′-AGCCCCGCACTTCCACCACCAGCTCCTCCA-3′, respectively) 
 
2)      Type of DNA sample- 1% of the DNA from a single dried blood spot. 
 
3)      Melting temperature- After denaturation (boiling for 15 minutes), the probe was blocked with Cot1 DNA (Invitrogen) for 20 minutes at 65°C. 
 
4)      Commercial name for DNA polymerase use- Chimeric PCR primer 
 
5)      How many cycle of replication- The PCR cycling profile was as follows: denaturation at 98°C for 10 minutes, 10 cycles at 97°C for 35 seconds, 64°C for 35 seconds, and 68°C for 4 minutes; 25 cycles at 97°C for 35 seconds, 64°C for 35 seconds, 68°C for 4 minutes plus a 20-second increment for each cycle; and a final extension at 68°C for 10 minutes. 
 

 

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ASSIGMENT 1
ASSIGMENT 1
 NURAMIRA SYAFIQA BT MOHD ZAHIR HUSSIN  (2017318287)

1.Primer sequence:  5' TACAATGTA CACATGGAAT 3' (sense), 5' GCAGTCTAGCGAAGAAGA 3' (sense), 5' CTTCTC CAATTGTCCCTCATA 3' (antisense), and 5' CGCCATAGTG CTTCCTGCTGCT 3' (antisense)

2.Type of DNA sample: Cloned HIV DNA (HXB2)

3.Melting temperature: 95°C

4.Commercial name for DNA polymerase used: Thermus aquaticus (Taq) DNA polymerase and Thermotoga maritima (ULTMA™) DNA polymerase

5. How many cycle of replication: 35 cycles

 

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ASSIGNMENT 1
ASSIGNMENT 1 
FARAH HANI FILZAH BINTI FAJIM JUFFA (2017970591)
AIN NUR NAJIHAH BT MUHD JUHARI (2017301321)
NURHIDAYATUL NAZUHA BT ZAMRI (2017735175) 
NOOR FAHANNA BT ZAIDAN (2017787463)
  a) Cyt bForward (FW) and Cyt b-Reverse (REV) oligonucleotide
b) cytochrome b gene
c)95  °C 
d) 
e) 35 cycles
 

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Assigm
Assigm
 

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Assignment 1.
Assignment 1. 
Nisa' Marini bt Abdul Jamil (2017337959)
Nur Shafiqah bt Mat Rofii (2017132381)
Nurul Nadiah bt Ismail (2017143103)
Azyan Anisah bt Ramli (2017998411

 

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Assignment 1
Assignment 1
Puteri Nur Syafiqah Bt Mohd Sukhimi (2017299224)
Norazira Bt Mohd Yusof (2017552451)
 
 
Primer sequences - forward primer and reverse primer, 5’ to 3’
Types of DNA sample - blood
Melting temperature - 95 degree celcius
Commercial name for DNA polymerase used - Taq polymerase
How many cycle of replication - 30 cycles
 

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1. Raja Nur Faqihah binti Raja Sharin (2017447894
1. Raja Nur Faqihah binti Raja Sharin (2017447894
2. Amirah Hanani Binti Abdul Zaki (2017447916)
3. Nur Najihah Binti Nasuha (2017447912)
4. Nur Syamim Athirah Binti Kamarul Bahrin (2017656762


ANSWER
1. 4 (G+C) + 2 (A+T)
2. MELTING TEMPERATURE IS 57°C AND ABOVE
3.SALMON SPERM DNA
4. TAQ POLYMERASE
5. 36 CYCLES



 

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Assignment
Assignment 
1. Nurul Athilah Binti Mohd Ariffin (2017656778)
2. Najwatul Aliya Bt Sabran (2017656788)
3. Nurul Shafiqa binti Zamri (2017656798)
4. SitiZulaikha binti Zulkefeli (2017656804)

PCR
1. Primer sequence
albu:CGGCGGCGGGCGGCGCGGGCTGGGCGGaaatgctgcacagaatccttg
albd:GCCCGGCTCCCGCCGgaaaagcatggtcgcctgtt
2.Type of DNA sample
Blood sample
3. Melting temperature
84 degree celcius
4. Commercialname of DNA
Amplitaq GOld DNA polymerase
5. How many cycle of replication
30
 

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Assignment 1 (PC
Assignment 1 (PC
1.Muhammad Firas Hidayat Bin Mohd Yusoff
(2017656802)
2.Muhammad Ammar Zulqarnain Bin Abdul Rahaman
(2017656766)

Answer:
1. Primer Sequence : miR-31-3p
2.Type of DNA sample : 2.7 microLitre complementary DNART product
3. Melting temperature : 59 degree celcius
4. DNA polymerase used : TaqMan Universal Master Mix
5. Number of cycle of replication : 40 cycles
 

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ASSIGNMENT 1
ASSIGNMENT 1
Amal Nurnadia Razali (2017447918)
Anis Syahirah Saifor Adzuan (2017447914)
Nurul Amirah Binti Rosdi (2017656792)
Ratu Mas Kinaz Binti Norlan (2017656794)
 

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Assignment
Assignment
Hazeeyah Nadheerah bt Zakaria 
-2017948271
Siti Nur Aisya bt Ibrahim  
-2017197343
Nurul Nadia bt Kairuddin 
-2017134235
Raja Fairuz Shafizah bt Raja Mohamad Aznan
-2017997131

Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis

1. Primer sequence 

- CYT b FW 5′-CCA TCC AAC ATC TCA GCA TGA TGA AA-3′

CYT b REV 5′-GCC CCT CAG AAT GAT ATT TGT CCT CA-3′

2. Type of DNA sample
     
- Meatball

3. Melting temperature - Tm

- 94 C

4. Commersial name of DNA polymerase used

- Mega-mix royal (optimized mixture of Taq polymerase, anti-Taq polymerase monoclonal antibodies in 2×reaction buffer (6 mM MgCl2 with 400 μM dNTPs

5. How many cycle of replication 

- 35 cycles
 

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PCR assignment 1
PCR assignment 1
Muhammad Hasni b. Rosli
-2017134357
Fauzan Haziq b. Mardin
-2017148657
Muhamad Syazwan b. Fazale
-2017726081
Ika Cemalia bt. Ahmad Rohimin
-2017501647

 Primer Sequence=  A forward primer, HLB-65 (5′- TCCTGAGAATTACACACAAAC), and a reverse primer, HLB66 (5′-TCTAAGTCTATCCTGTAACCC).
 Type of DNA sample= HLB-infected tissue.
 Melting Temperature(Tm)=  94°C for 2 min; followed by 40 cycles at 94°C for 20 s.
 Commercial Name for DNA Polymerase used=   SpeedSTAR HS DNA polymerase and  Go Taq Flexi DNA polymerase.
 Cycles of Replication= 40 cycles.
 

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ASSIGNMENT 1
ASSIGNMENT 1
Hazeeyah Nadheerah bt Zakaria 
-2017948271
Siti Nur Aisya bt Ibrahim  
-2017197343
Nurul Nadia bt Kairuddin 
-
Raja Fairuz Shafizah bt Raja Mohamad Aznan
-2017997131
 

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Polymerase Chain Reaction Assigment
Polymerase Chain Reaction Assigment
NURAIN SIDIIQIN BINTI MOHAMAD ARBI (2017656806)
NUR IZYAN ‘IZZATI BT ZAHRAN (2017656772)
NURUL SYUHADA BINTI YUSHAZA (2017447906)
WAN NURHAZIRAH FATINI BT WAN HASSAN (2017656796)

1. Primer sequence- Oligonucleotide primer
2. Type of DNA sample- linear strand DNA obtained from tissues including, blood films and body fluid
3. Melting temperature - Tm
  - Denaturation : 94-95 degree celcius
4. Commercial name for DNA polymerasa used
  - Taq denaturation
5. How many cycle of replication
  - Enzyme can withstand for 30 or more cycles without being damages.
  - In a cycle it involved 3 steps
i) Denaturation
ii) Annealing
iii) Extension
 

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Assigment 1
Assigment  1
Nurzahra Binti Amin Nasir (2017447898), Asmaa’ Zuhaira Binti Asri (2017447902), Anis Ilyani Binti Kamarruddin (2017656758), Nurfarina Afza Binti Aminuddin (2017447922)

1)primer sequence – refer table 1
2) type of DNA sample - Escherichia coli
3)melting temperature- Tm - annealing at 55C for 20 s
4)commercial name for DNA polymerase used - Taq polymerase
5) how many cycle of replication - 30 cycles

 

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ASSIGNMENT 1
ASSIGNMENT 1 
Farah Hani Filzah bt Fajim Juffa (2017970591)
Ain Nur Najihah bt Muhd Juhari (2017301321)
Nurhidayatul Nazuha bt Zamri (2017735175)
Noor Fahanna bt Zaidan (2017787463)

a) primer sequence : Cyt bForward (FW) and Cyt b-Reverse (REV) oligonucleotide
b) target DNA sample : cytochrome b gene
c) melting temperature : 95  °C 
d) commercial name for DNA polymerase used :
e) cycle of replication: 35 cycle  
 

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Assignmen
Assignmen
 

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Assignment 1
Assignment 1
1.Sofia Zaireen Bt Shahrizman (2017160771)
2.Nur Azila Suhana Bt Mat Yunos (2017726587)
3.Nurul Farhana Bt Md Noor (2017507575)
A)primer sequences: primer 3 software and Minder software.refer table 1
B)type of DNA sample: Gossypium hirsutum
C)melting temperature: 60-+1°c
D)commericial name for DNA polymerase used:Platinum Taq DNA polymerase
E)cycle replication:40 cycle

 

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5 elements of PCR reflecting the replication process
5 elements of PCR reflecting the replication process
name , id 
1. Priscillia Durrie Anak Dasan , 2017921981
2. Nurin Nazirah Binti Mokhtar, 2017978745
3. Nurfarahain Binti Mohamad Fauzi, 2017369837


answers :
1. primer sequence - 5' to 3' , 3' to 5
2. type of dna sample - E.coli dna polymerase
3. melting temperature - 90 until 97 °C
4. commercial name for DNA polymerase used = Taq polymerase (Thermus Aquaticus) 
5. how many cycle in replication - 30 cycles 

2 comments

Farizan Aris 2d
Where's ur group member?

Farizan Aris 2d
Wheres the primer sequence?


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ASSIGNEMENT 1 (PCR METHOD-THE 5 ELEMETS)
ASSIGNEMENT 1 (PCR METHOD-THE 5 ELEMETS)
AHMAD HILMI HAZIQ BVINA BD HAMID ()
FADHIL BIN AHMAD
SYED ZAIN BIN SYED
DANIAL
 

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Assignment 1
Assignment 1
NUR FATIN AFIQAH BINTI NOR RAHIM (2017917491)
NORHIDAYU BINTI ROSLI (2017951841)
WAN NURIN AFIQEEN BINTI WAN MAIZUL KHAR (2017702491)
MICHEAL ALLEN ANAK MARY (2017988489)


The answerwas highlighted and commented in the pdf document. One of the primer sequence is:
5’-TGATTGACCTTTCCAGAGCAAG-3’ 
(Based on table S1)
 

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Assignment 1
Assignment 1
1. Primer sequence: refer table 3 page 230
2. Type of DNA sample : Lactobacillus strains of casei group (L.casei, L.paracasei, L.rhamnosus) and prophage
3. Melting temperature : 50, 55 or 60°C for 2 minutes
4. Commercial name for DNA Polymerase used : DNA taq Polymerase
6. No of cycle of replication : 35 cycle
1. Nurul Hamiemah bt Mohamad (2017377683)
2. Nurun Nisa' Nardalina bt Sa'ad (2017522799)
3. Lutfia Ilsahaida bt Abdul Malik (2017593611)
 

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ASSIGNMENT 1(PCR METHOD-The 5 elements)
ASSIGNMENT 1(PCR METHOD-The 5 elements)
1.Fadhil bin Ahmad 
(2017119147)
2.Ahmad Hilmi Haziq bin Abd Hamid
(2017711963)
3.Muhamad Danial Bin Mohamed
(2017773721)
4.Syed Zain Bin Syed Idros
(2017327219).

1-Primer sequences of sample selected- 
5' -cagatgaagaagaatgaggcg-3' 
2-Type of DNA Sample - DNA selected from a chicken meat
3-Melting temperature - 95 Celcius
4- Commercial name for DNA Polymerase - Takara Taq DNA Polymerase
5- DNA Cycles-  25 cycles
 

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Assignment
Assignment 
1. KAMALEA AMALEENA FARHANA BINTI KAMAL RAMLEE (2017533503)
2. NIK FATIN FARISYA BINTI HARUN (2017981947)
3.SITI ZULAIKHA MAKHDAR (2017706727)

1) primer sequence : P20 and P21, P38 and P39
2) type of DNA sample : islet cells from Human tissues, pancreata from chosen mice.
3)melting temperature- Tm : 95°C in 15 min
4)commercial name for DNA polymerase used :P20 and P21 polymerase, P38 and P39 polymerase
5) how many cycle of replication : 15 cycles for 1 step, 40 cycles for step 2.
 

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PCR method :)
PCR method :)
AINNUR SHAFIRAH (2017173693)
INTAN NAZYRA (2017105275)
RUHULLATIF (2017157579)
1) Primer sequence = pol genes
2) Type of DNA sample = Ecoli from soil
3) Melting temperature = 90C
4) Commercial name =  ULTIMA DNA polymerase 
5) How many cycle of replication = 3 cycle
1 comment

Farizan Aris 2d
3 cycles ONLY?


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PCR
PCR 
 

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TOPIC 2
 TOPIC 2
 

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DNA STRUCTURE AFTER SHAKING
DNA STRUCTURE AFTER SHAKING
Nurul Hamiemah bt Mohamad, AS244
 

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Melting, renaturation or reanneling
Melting, renaturation or reanneling
Nur Fatin Afiqah
AS2442B
 

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ASIGNMENT 1
 ASIGNMENT 1
 

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PADLET CODE
PADLET CODE
 

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TOPIC 1
TOPIC 1
Organisation, structure and function of genetic material
 

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📎 Video


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📎 Video


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NOW EV
 NOW EVERYONE CAN TALK!
 

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# data[].attributes.body
1. Nur Shafiqah bt Mat Rofii

(2017132381)

2. Azyan Anisah Binti Ramli (2017998411)

3. Nurul Nadiah Binti Ismail (2017143103)

5. Nisa' Marini binti Abdul Jamil (2017337959)

1) PRIMER sequence: 5’ to 3’ and 3’ to 5’ sequence ( A sequence that complementary to the template)
2) Type of DNA sample: Bacterial DNA
3) Melting Temperature: 95 degree celcius.
4) Commercial name for DNA polymerase used: Taq DNA Polymerase
5) How many cycle of replication: can be up to 40 cycle.

# data[].attributes.body
ABDUL HAZIQ IKHWAN BIN ABD HALID HAN- 2017583241
KHAIRUL ANWAR BIN AHMAD-2017119683
AIMAN HAIQAL BIN AZHARI-2017381755
WAN ALIF AFIQ BIN WAN NOR RUDDIN-2017776423

PCR
1. Forward primer and reverse primer, 5’ to 3’
2.  DNA of honey bee and mantis
3. 54.5-59.5 °C
4. Taq polymerase
5.  30 cycles

# data[].attributes.attachment_link.title
Polymerase chain reaction with nearby primers.pdf

# data[].attributes.body
WAN NUR SYAMIMI 2017987915
ZULAIKHA HAZIQAH BINTI MOHD ZULKIFLI 2017947093
NOR SYAFIKAH BINTI MOHD NASIR 2017982441SITI NOR SUHAILAH BINTI MAT SAAT 2017972081

1. DNA primer sequence - refer Table 1.
2. DNA sample - Genes in Gossypium hirusutum
3. Commercial name - Platinum Taq DNA polymerase (Invitrogen)
4. Replication sample - 40 cycle of 15s at 94Celcius, 10s at 60Celcius and 15s at 72 Celcius
5. Melting point - 60 Celcius

# data[].attributes.body
Nur atikah hazirah bt zarimi (2017745517)

Rohadatul aisy binti zolmi (2017172273)

Siti nur aminara binti mohd azlan (2017153275)

Nurul amirah binti mohamad farook (2017921391)

1)      DNA primer sequence- Standard primer PCR reactions were performed using the c and f primers (5′-GCTCAGCTCCGTTTCGGTTTCACTTCCGGT-3′; 5′-AGCCCCGCACTTCCACCACCAGCTCCTCCA-3′, respectively)

2)      Type of DNA sample- 1% of the DNA from a single dried blood spot.

3)      Melting temperature- After denaturation (boiling for 15 minutes), the probe was blocked with Cot1 DNA (Invitrogen) for 20 minutes at 65°C.

4)      Commercial name for DNA polymerase use- Chimeric PCR primer

5)      How many cycle of replication- The PCR cycling profile was as follows: denaturation at 98°C for 10 minutes, 10 cycles at 97°C for 35 seconds, 64°C for 35 seconds, and 68°C for 4 minutes; 25 cycles at 97°C for 35 seconds, 64°C for 35 seconds, 68°C for 4 minutes plus a 20-second increment for each cycle; and a final extension at 68°C for 10 minutes.

# data[].attributes.body
NURAMIRA SYAFIQA BT MOHD ZAHIR HUSSIN (2017318287)

1.Primer sequence:  5' TACAATGTA CACATGGAAT 3' (sense), 5' GCAGTCTAGCGAAGAAGA 3' (sense), 5' CTTCTC CAATTGTCCCTCATA 3' (antisense), and 5' CGCCATAGTG CTTCCTGCTGCT 3' (antisense)

2.Type of DNA sample: Cloned HIV DNA (HXB2)

3.Melting temperature: 95°C

4.Commercial name for DNA polymerase used: Thermus aquaticus (Taq) DNA polymerase and Thermotoga maritima (ULTMA™) DNA polymerase

5. How many cycle of replication: 35 cycles

# data[].attributes.body
FARAH HANI FILZAH BINTI FAJIM JUFFA (2017970591)
AIN NUR NAJIHAH BT MUHD JUHARI (2017301321)
NURHIDAYATUL NAZUHA BT ZAMRI (2017735175)
NOOR FAHANNA BT ZAIDAN (2017787463)
a) Cyt bForward (FW) and Cyt b-Reverse (REV) oligonucleotide
b) cytochrome b gene
c)95  °C
d)
e) 35 cycles

# data[].attributes.attachment_link.title
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis.pdf

# data[].attributes.body
Nisa' Marini bt Abdul Jamil (2017337959)
Nur Shafiqah bt Mat Rofii (2017132381)
Nurul Nadiah bt Ismail (2017143103)
Azyan Anisah bt Ramli (2017998411

# data[].attributes.body
Puteri Nur Syafiqah Bt Mohd Sukhimi (2017299224)

Norazira Bt Mohd Yusof (2017552451)

Primer sequences - forward primer and reverse primer, 5’ to 3’

Types of DNA sample - blood

Melting temperature - 95 degree celcius

Commercial name for DNA polymerase used - Taq polymerase

How many cycle of replication - 30 cycles

# data[].attributes.headline
1. Raja Nur Faqihah binti Raja Sharin (2017447894

# data[].attributes.subject
1. Raja Nur Faqihah binti Raja Sharin (2017447894

# data[].attributes.body
2. Amirah Hanani Binti Abdul Zaki (2017447916)
3. Nur Najihah Binti Nasuha (2017447912)
4. Nur Syamim Athirah Binti Kamarul Bahrin (2017656762

ANSWER
1. 4 (G+C) + 2 (A+T)
2. MELTING TEMPERATURE IS 57°C AND ABOVE
3.SALMON SPERM DNA
4. TAQ POLYMERASE
5. 36 CYCLES

# data[].attributes.body
1. Nurul Athilah Binti Mohd Ariffin (2017656778)
2. Najwatul Aliya Bt Sabran (2017656788)
3. Nurul Shafiqa binti Zamri (2017656798)
4. SitiZulaikha binti Zulkefeli (2017656804)

PCR
1. Primer sequence
albu:CGGCGGCGGGCGGCGCGGGCTGGGCGGaaatgctgcacagaatccttg
albd:GCCCGGCTCCCGCCGgaaaagcatggtcgcctgtt
2.Type of DNA sample
Blood sample
3. Melting temperature
84 degree celcius
4. Commercialname of DNA
Amplitaq GOld DNA polymerase
5. How many cycle of replication
30

# data[].attributes.body
1.Muhammad Firas Hidayat Bin Mohd Yusoff
(2017656802)
2.Muhammad Ammar Zulqarnain Bin Abdul Rahaman
(2017656766)

Answer:
1. Primer Sequence : miR-31-3p
2.Type of DNA sample : 2.7 microLitre complementary DNART product
3. Melting temperature : 59 degree celcius
4. DNA polymerase used : TaqMan Universal Master Mix
5. Number of cycle of replication : 40 cycles

# data[].attributes.body
Amal Nurnadia Razali (2017447918)
Anis Syahirah Saifor Adzuan (2017447914)
Nurul Amirah Binti Rosdi (2017656792)
Ratu Mas Kinaz Binti Norlan (2017656794)

Answer :

(1) primer sequence

-foward and reverse primer starts from 5’ to 3’

(2) type of dna sample

-blood

-solitary of hair

(3) melting temperature

-95 celcius

(4)commercial name dna polymerase

-Taq Dna Polymerase

(5)how many cycle replications

-20 to 40 cycles

# data[].attributes.body
Hazeeyah Nadheerah bt Zakaria
-2017948271
Siti Nur Aisya bt Ibrahim
-2017197343
Nurul Nadia bt Kairuddin
-2017134235
Raja Fairuz Shafizah bt Raja Mohamad Aznan
-2017997131

Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis

1. Primer sequence

- CYT b FW 5′-CCA TCC AAC ATC TCA GCA TGA TGA AA-3′

CYT b REV 5′-GCC CCT CAG AAT GAT ATT TGT CCT CA-3′

2. Type of DNA sample

- Meatball

3. Melting temperature - Tm

- 94 C

4. Commersial name of DNA polymerase used

- Mega-mix royal (optimized mixture of Taq polymerase, anti-Taq polymerase monoclonal antibodies in 2×reaction buffer (6 mM MgCl2 with 400 μM dNTPs

5. How many cycle of replication

- 35 cycles

# data[].attributes.body
Muhammad Hasni b. Rosli
-2017134357
Fauzan Haziq b. Mardin
-2017148657
Muhamad Syazwan b. Fazale
-2017726081
Ika Cemalia bt. Ahmad Rohimin
-2017501647

1.  Primer Sequence=  A forward primer, HLB-65 (5′- TCCTGAGAATTACACACAAAC), and a reverse primer, HLB66 (5′-TCTAAGTCTATCCTGTAACCC).

2.  Type of DNA sample= HLB-infected tissue.

3.  Melting Temperature(Tm)=  94°C for 2 min; followed by 40 cycles at 94°C for 20 s.

4.  Commercial Name for DNA Polymerase used=   SpeedSTAR HS DNA polymerase and  Go Taq Flexi DNA polymerase.

5.  Cycles of Replication= 40 cycles.

# data[].attributes.attachment_link.title
In Planta Distribution of ‘Candidatus Liberibacter asiaticus’  as Revealed by Polymerase Chain Reaction (PCR)  and Real-Time PCR.pdf

# data[].attributes.body
Hazeeyah Nadheerah bt Zakaria

-2017948271

Siti Nur Aisya bt Ibrahim
-2017197343
Nurul Nadia bt Kairuddin
-
Raja Fairuz Shafizah bt Raja Mohamad Aznan

-2017997131

# data[].attributes.body
NURAIN SIDIIQIN BINTI MOHAMAD ARBI (2017656806)
NUR IZYAN ‘IZZATI BT ZAHRAN (2017656772)
NURUL SYUHADA BINTI YUSHAZA (2017447906)
WAN NURHAZIRAH FATINI BT WAN HASSAN (2017656796)

1. Primer sequence- Oligonucleotide primer
2. Type of DNA sample- linear strand DNA obtained from tissues including, blood films and body fluid
3. Melting temperature - Tm
- Denaturation : 94-95 degree celcius
4. Commercial name for DNA polymerasa used
- Taq denaturation
5. How many cycle of replication
- Enzyme can withstand for 30 or more cycles without being damages.
- In a cycle it involved 3 steps
i) Denaturation
ii) Annealing
iii) Extension

# data[].attributes.body
Nurzahra Binti Amin Nasir (2017447898), Asmaa’ Zuhaira Binti Asri (2017447902), Anis Ilyani Binti Kamarruddin (2017656758), Nurfarina Afza Binti Aminuddin (2017447922)

1)primer sequence – refer table 1

2) type of DNA sample - Escherichia coli

3)melting temperature- Tm - annealing at 55C for 20 s

4)commercial name for DNA polymerase used - Taq polymerase

5) how many cycle of replication - 30 cycles

# data[].attributes.body
Farah Hani Filzah bt Fajim Juffa (2017970591)
Ain Nur Najihah bt Muhd Juhari (2017301321)
Nurhidayatul Nazuha bt Zamri (2017735175)
Noor Fahanna bt Zaidan (2017787463)

a) primer sequence : Cyt bForward (FW) and Cyt b-Reverse (REV) oligonucleotide
b) target DNA sample : cytochrome b gene
c) melting temperature : 95  °C
d) commercial name for DNA polymerase used :
e) cycle of replication: 35 cycle

# data[].attributes.attachment_link.title
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis.pdf

# data[].attributes.body
1.Sofia Zaireen Bt Shahrizman (2017160771)
2.Nur Azila Suhana Bt Mat Yunos (2017726587)
3.Nurul Farhana Bt Md Noor (2017507575)
A)primer sequences: primer 3 software and Minder software.refer table 1
B)type of DNA sample: Gossypium hirsutum
C)melting temperature: 60-+1°c
D)commericial name for DNA polymerase used:Platinum Taq DNA polymerase
E)cycle replication:40 cycle

# data[].attributes.headline
5 elements of PCR reflecting the replication process

# data[].attributes.subject
5 elements of PCR reflecting the replication process

# data[].attributes.body
name , id
1. Priscillia Durrie Anak Dasan , 2017921981
2. Nurin Nazirah Binti Mokhtar, 2017978745
3. Nurfarahain Binti Mohamad Fauzi, 2017369837

answers :
1. primer sequence - 5' to 3' , 3' to 5
2. type of dna sample - E.coli dna polymerase
3. melting temperature - 90 until 97 °C
4. commercial name for DNA polymerase used = Taq polymerase (Thermus Aquaticus)
5. how many cycle in replication - 30 cycles

# data[].attributes.headline
ASSIGNEMENT 1 (PCR METHOD-THE 5 ELEMETS)

# data[].attributes.subject
ASSIGNEMENT 1 (PCR METHOD-THE 5 ELEMETS)

# data[].attributes.body
AHMAD HILMI HAZIQ BVINA BD HAMID ()
FADHIL BIN AHMAD
SYED ZAIN BIN SYED
DANIAL

# data[].attributes.body
NUR FATIN AFIQAH BINTI NOR RAHIM (2017917491)
NORHIDAYU BINTI ROSLI (2017951841)
WAN NURIN AFIQEEN BINTI WAN MAIZUL KHAR (2017702491)
MICHEAL ALLEN ANAK MARY (2017988489)

The answerwas highlighted and commented in the pdf document. One of the primer sequence is:
5’-TGATTGACCTTTCCAGAGCAAG-3’
(Based on table S1)

# data[].attributes.body
1. Primer sequence: refer table 3 page 230
2. Type of DNA sample : Lactobacillus strains of casei group (L.casei, L.paracasei, L.rhamnosus) and prophage
3. Melting temperature : 50, 55 or 60°C for 2 minutes
4. Commercial name for DNA Polymerase used : DNA taq Polymerase
6. No of cycle of replication : 35 cycle
1. Nurul Hamiemah bt Mohamad (2017377683)
2. Nurun Nisa' Nardalina bt Sa'ad (2017522799)
3. Lutfia Ilsahaida bt Abdul Malik (2017593611)

# data[].attributes.body
1.Fadhil bin Ahmad
(2017119147)
2.Ahmad Hilmi Haziq bin Abd Hamid
(2017711963)
3.Muhamad Danial Bin Mohamed
(2017773721)
4.Syed Zain Bin Syed Idros
(2017327219).

1-Primer sequences of sample selected-
5' -cagatgaagaagaatgaggcg-3'
2-Type of DNA Sample - DNA selected from a chicken meat
3-Melting temperature - 95 Celcius
4- Commercial name for DNA Polymerase - Takara Taq DNA Polymerase
5- DNA Cycles- 25 cycles

# data[].attributes.body
1. KAMALEA AMALEENA FARHANA BINTI KAMAL RAMLEE (2017533503)
2. NIK FATIN FARISYA BINTI HARUN (2017981947)
3.SITI ZULAIKHA MAKHDAR (2017706727)

1) primer sequence : P20 and P21, P38 and P39
2) type of DNA sample : islet cells from Human tissues, pancreata from chosen mice.
3)melting temperature- Tm : 95°C in 15 min
4)commercial name for DNA polymerase used :P20 and P21 polymerase, P38 and P39 polymerase
5) how many cycle of replication : 15 cycles for 1 step, 40 cycles for step 2.

# data[].attributes.body
AINNUR SHAFIRAH (2017173693)
INTAN NAZYRA (2017105275)
RUHULLATIF (2017157579)
1) Primer sequence = pol genes
2) Type of DNA sample = Ecoli from soil
3) Melting temperature = 90C
4) Commercial name =  ULTIMA DNA polymerase
5) How many cycle of replication = 3 cycle

# data[].attributes.attachment_link.title
DNA polymerases as useful reagents for biotechnology – the history of developmental research in the field.pdf